CN108715808A - A kind of device for growth of tumour cell Inhibition test - Google Patents
A kind of device for growth of tumour cell Inhibition test Download PDFInfo
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- 230000005764 inhibitory process Effects 0.000 title claims abstract description 17
- 210000004881 tumor cell Anatomy 0.000 title description 9
- 230000012010 growth Effects 0.000 title description 4
- 238000002474 experimental method Methods 0.000 claims abstract description 24
- 230000004565 tumor cell growth Effects 0.000 claims abstract description 18
- 238000004321 preservation Methods 0.000 claims abstract description 14
- 238000005192 partition Methods 0.000 claims abstract description 13
- 238000005485 electric heating Methods 0.000 claims abstract description 11
- 239000011521 glass Substances 0.000 claims abstract description 10
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- 229910052918 calcium silicate Inorganic materials 0.000 claims description 2
- 239000000378 calcium silicate Substances 0.000 claims description 2
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 10
- 238000010438 heat treatment Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 6
- 230000005760 tumorsuppression Effects 0.000 abstract description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 28
- 229910002092 carbon dioxide Inorganic materials 0.000 description 14
- 239000001569 carbon dioxide Substances 0.000 description 14
- 229940079593 drug Drugs 0.000 description 8
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- 230000009946 DNA mutation Effects 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- JHXCINJSAAFBDH-UHFFFAOYSA-N [Ca].O[Si](O)(O)O Chemical compound [Ca].O[Si](O)(O)O JHXCINJSAAFBDH-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 230000002113 chemopreventative effect Effects 0.000 description 1
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Abstract
本发明公开的属于肿瘤抑制实验技术领域，具体为一种用于肿瘤细胞生长抑制实验的装置，包括箱体，所述箱体的内腔均匀纵向插接有三个隔板，所述隔板与箱体的内腔通过螺钉固定连接，三个所述隔板将箱体分隔为四个单元实验室，所述单元实验室的前侧铰接有取放门，所述取放门的前壁中部横向开设有通槽，且通槽内部嵌入有观察玻璃窗，所述单元实验室的顶端嵌入有顶盖，通过控制电流的大小控制电加热管的功率，能够适用于多种不同的温度加热保温方式；通过进气流量可调的方式能够改变进气量；通过橡胶塞的设置，能够通过注射器实时向实验器皿注射实验药剂；通过观察玻璃窗的设置，能够实时观察内部的实验情况，且不影响其正常实验。
The invention disclosed by the invention belongs to the technical field of tumor suppression experiments, and specifically relates to a device for tumor cell growth inhibition experiments, which includes a box body, and three partitions are evenly and longitudinally inserted into the inner cavity of the box body, and the partition boards are connected with the The inner cavity of the box is fixedly connected by screws, and the three partitions divide the box into four unit laboratories. The front side of the unit laboratory is hinged with a pick-and-place door, and the middle part of the front wall of the pick-and-place door is A through groove is opened horizontally, and an observation glass window is embedded inside the through groove, and a top cover is embedded on the top of the unit laboratory, and the power of the electric heating tube is controlled by controlling the size of the current, which can be applied to heating and heat preservation at various temperatures method; the air intake volume can be changed by adjusting the air intake flow rate; through the setting of the rubber stopper, the experimental medicine can be injected into the experimental vessel in real time through the syringe; through the setting of the observation glass window, the internal experimental situation can be observed in real time without Affect its normal experiment.
Description
技术领域technical field
本发明涉及肿瘤抑制实验技术领域，具体为一种用于肿瘤细胞生长抑制实验的装置。The invention relates to the technical field of tumor suppression experiments, in particular to a device for tumor cell growth suppression experiments.
背景技术Background technique
在抗肿瘤研究中，人们提出了肿瘤多步骤、DNA突变、细胞凋亡、细胞周期核心机制、细胞周期启动及多条信号转导途径参与等许多理论和学说。In anti-tumor research, many theories and theories have been put forward, such as multi-step tumor, DNA mutation, apoptosis, core mechanism of cell cycle, initiation of cell cycle and participation of multiple signal transduction pathways.
抗肿瘤药物的研究开发随着理论的更新和技术的进步，已从以核酸等为靶点的杀伤型细胞毒药转向对肿瘤新靶点的研究。包括化学预防药、分化诱导剂、凋亡诱导剂、信号传导阻滞剂、血管生成抑制剂、生物调节剂等。The research and development of anti-tumor drugs has shifted from killing cytotoxic drugs targeting nucleic acid and the like to the research on new tumor targets with the updating of the theory and the advancement of technology. Including chemopreventive drugs, differentiation inducers, apoptosis inducers, signal transduction blockers, angiogenesis inhibitors, biological regulators, etc.
在抗肿瘤开发研究当中，检测药物对肿瘤细胞生长抑制的实验研究是一项重要的研究项目。现有的通常采用染料排斥法检测药物对肿瘤细胞生长抑制。In the anti-tumor development research, the experimental research of detecting the inhibition of tumor cell growth by drugs is an important research project. Existing methods usually use the dye exclusion method to detect the inhibition of tumor cell growth by drugs.
现有的实验仪器当中，没有专门整体的对于肿瘤细胞生长抑制实验配套的装置，在进行实验时，无法有效地控制二氧化碳浓度以及温度，使得肿瘤细胞在受药培养时不能达到预期的效果，进而影响整个实验的结果评定，从而导致实验精准度低下。Among the existing experimental instruments, there is no dedicated overall device for tumor cell growth inhibition experiments. During the experiment, the concentration of carbon dioxide and temperature cannot be effectively controlled, so that the tumor cells cannot achieve the expected effect when they are cultured with drugs. Affect the evaluation of the results of the entire experiment, resulting in low accuracy of the experiment.
发明内容Contents of the invention
本发明的目的在于提供一种用于肿瘤细胞生长抑制实验的装置，以解决上述背景技术中提出的现有的实验仪器当中，没有专门整体的对于肿瘤细胞生长抑制实验配套的装置，在进行实验时，无法有效地控制二氧化碳浓度以及温度，使得肿瘤细胞在受药培养时不能达到预期的效果的问题。The object of the present invention is to provide a kind of device that is used for tumor cell growth inhibition experiment, in order to solve in the existing experimental apparatus that proposes in the above-mentioned background technology, there is no special integral device for tumor cell growth inhibition experiment matching, when carrying out experiment At the same time, the carbon dioxide concentration and temperature cannot be effectively controlled, so that the tumor cells cannot achieve the expected effect when they are cultured with drugs.
为实现上述目的，本发明提供如下技术方案：一种用于肿瘤细胞生长抑制实验的装置，包括箱体，所述箱体的内腔均匀纵向插接有三个隔板，所述隔板与箱体的内腔通过螺钉固定连接，三个所述隔板将箱体分隔为四个单元实验室，所述单元实验室的前侧铰接有取放门，所述取放门的前壁中部横向开设有通槽，且通槽内部嵌入有观察玻璃窗，所述单元实验室的顶端嵌入有顶盖，所述顶盖的中部嵌入有橡胶塞，所述顶盖的左侧纵向插接有进气管，所述进气管的管路上安装有流量控制阀，所述流量控制阀位于顶盖的上侧，所述顶盖的右侧纵向插接有出气管，所述橡胶塞位于进气管、出气管之间，所述单元实验室的底部卡接有电流调控器，所述单元实验室的内壁通过粘接剂粘接有保温腔，所述保温腔的内壁通过卡扣环向卡接有电加热管，所述电加热管与电流调控器电性连接，所述保温腔的内腔底部中部通过螺钉连接有立柱，所述立柱的顶端焊接有载料盘，且载料盘的顶部支撑有实验器皿。In order to achieve the above object, the present invention provides the following technical solutions: a device for tumor cell growth inhibition experiments, including a box, the inner cavity of the box is evenly and longitudinally inserted with three partitions, and the partitions are connected to the box. The inner cavity of the body is fixedly connected by screws, and the three partitions divide the box body into four unit laboratories. The front side of the unit laboratory is hinged with a pick-and-place door, and the middle part of the front wall of the pick-and-place door is horizontal A through groove is opened, and an observation glass window is embedded inside the through groove, a top cover is embedded in the top of the unit laboratory, a rubber plug is embedded in the middle of the top cover, and an inlet is inserted longitudinally on the left side of the top cover. An air pipe, a flow control valve is installed on the pipeline of the air intake pipe, the flow control valve is located on the upper side of the top cover, the right side of the top cover is longitudinally inserted with an air outlet pipe, and the rubber plug is located on the air intake pipe, outlet Between the trachea, the bottom of the unit laboratory is clamped with a current regulator, the inner wall of the unit laboratory is bonded with a heat preservation cavity through an adhesive, and the inner wall of the heat preservation cavity is clamped with an electric current regulator through a buckle ring. Heating tube, the electric heating tube is electrically connected with the current regulator, the middle part of the bottom of the inner cavity of the heat preservation chamber is connected with a column by screws, the top of the column is welded with a loading tray, and the top of the loading tray is supported by a Labware.
优选的，所述观察玻璃窗的高度与实验器皿的高度相同。Preferably, the height of the observation glass window is the same as that of the experimental vessel.
优选的，所述顶盖与橡胶塞的之间的尺寸配合方式为过盈配合。Preferably, the dimensional fit between the top cover and the rubber stopper is an interference fit.
优选的，所述进气管的底端伸入至实验器皿的内腔中部。Preferably, the bottom end of the air inlet pipe extends into the middle of the inner cavity of the experimental vessel.
优选的，所述出气管的底端连接有扩口集气罩。Preferably, the bottom end of the air outlet pipe is connected with a flaring air collecting hood.
优选的，所述出气管的内腔上侧嵌入有防尘海绵塞。Preferably, a dust-proof sponge plug is embedded in the upper side of the inner cavity of the outlet pipe.
优选的，所述电流调控器为滑动变阻式电流调控器。Preferably, the current regulator is a sliding rheostat current regulator.
优选的，所述保温腔为硅酸钙保温腔。Preferably, the insulation chamber is a calcium silicate insulation chamber.
与现有技术相比，本发明的有益效果是：Compared with prior art, the beneficial effect of the present invention is:
(1)通过控制电流的大小控制电加热管的功率，能够适用于多种不同的温度加热保温方式；(1) By controlling the power of the electric heating tube by controlling the size of the current, it can be applied to a variety of different temperature heating and heat preservation methods;
(2)通过进气流量可调的方式能够改变进气量；(2) The air intake volume can be changed by adjusting the air intake flow rate;
(3)通过橡胶塞的设置，能够通过注射器实时向实验器皿注射实验药剂；(3) Through the setting of the rubber stopper, the experimental medicine can be injected into the experimental vessel in real time through the syringe;
(4)通过观察玻璃窗的设置，能够实时观察内部的实验情况，且不影响其正常实验。(4) Through the setting of the observation glass window, the internal experimental situation can be observed in real time without affecting its normal experiment.
附图说明Description of drawings
图1为本发明结构示意图；Fig. 1 is a structural representation of the present invention;
图2为本发明图1的剖视结构示意图。FIG. 2 is a schematic cross-sectional structure diagram of FIG. 1 in the present invention.
图中：1箱体、2隔板、3取放门、4观察玻璃窗、5顶盖、6橡胶塞、7进气管、8流量控制阀、9出气管、10电流调控器、11电加热管、12保温腔、13立柱、14实验器皿。In the figure: 1 box body, 2 partitions, 3 access door, 4 observation glass window, 5 top cover, 6 rubber plug, 7 air inlet pipe, 8 flow control valve, 9 air outlet pipe, 10 current regulator, 11 electric heating Tube, 12 insulation chambers, 13 columns, 14 experimental vessels.
具体实施方式Detailed ways
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
请参阅图1-2，本发明提供一种技术方案：一种用于肿瘤细胞生长抑制实验的装置，包括箱体1，箱体1的内腔均匀纵向插接有三个隔板2，隔板2与箱体1的内腔通过螺钉固定连接，三个隔板2将箱体1分隔为四个单元实验室，单元实验室的前侧铰接有取放门3，取放门3的前壁中部横向开设有通槽，且通槽内部嵌入有观察玻璃窗4，单元实验室的顶端嵌入有顶盖5，顶盖5的中部嵌入有橡胶塞6，顶盖5的左侧纵向插接有进气管7，进气管7的管路上安装有流量控制阀8，流量控制阀8位于顶盖5的上侧，顶盖5的右侧纵向插接有出气管9，橡胶塞6位于进气管7、出气管9之间，单元实验室的底部卡接有电流调控器10，单元实验室的内壁通过粘接剂粘接有保温腔12，保温腔12的内壁通过卡扣环向卡接有电加热管11，电加热管11与电流调控器10电性连接，保温腔12的内腔底部中部通过螺钉连接有立柱13，立柱13的顶端焊接有载料盘，且载料盘的顶部支撑有实验器皿14。Please refer to Figures 1-2, the present invention provides a technical solution: a device for tumor cell growth inhibition experiments, including a box 1, the inner cavity of the box 1 is evenly inserted with three partitions 2 longitudinally, the partitions 2 and the inner cavity of the box body 1 are fixedly connected by screws, and the box body 1 is divided into four unit laboratories by three partitions 2. The front side of the unit laboratory is hinged with a pick-and-place door 3, and the front wall of the pick-and-place door 3 A through groove is opened horizontally in the middle, and an observation glass window 4 is embedded inside the through groove, a top cover 5 is embedded in the top of the unit laboratory, a rubber plug 6 is embedded in the middle of the top cover 5, and a Intake pipe 7, a flow control valve 8 is installed on the pipeline of the air inlet pipe 7, the flow control valve 8 is located on the upper side of the top cover 5, the right side of the top cover 5 is longitudinally inserted with an air outlet pipe 9, and the rubber plug 6 is located on the air intake pipe 7 , between the air outlet pipes 9, the bottom of the unit laboratory is clamped with a current regulator 10, the inner wall of the unit laboratory is bonded with a heat preservation chamber 12 through an adhesive, and the inner wall of the heat preservation chamber 12 is connected with an electric current controller through a buckle ring. The heating tube 11, the electric heating tube 11 is electrically connected with the current regulator 10, the middle part of the inner cavity bottom of the heat preservation chamber 12 is connected with a column 13 by screws, the top of the column 13 is welded with a loading tray, and the top of the loading tray is supported by a Labware14.
其中，观察玻璃窗4的高度与实验器皿14的高度相同，顶盖5与橡胶塞6的之间的尺寸配合方式为过盈配合，进气管7的底端伸入至实验器皿14的内腔中部，出气管9的底端连接有扩口集气罩，出气管9的内腔上侧嵌入有防尘海绵塞，电流调控器10为滑动变阻式电流调控器，保温腔12为硅酸钙保温腔。Wherein, the height of the observation glass window 4 is the same as that of the experimental vessel 14, the size fit between the top cover 5 and the rubber stopper 6 is an interference fit, and the bottom end of the air inlet pipe 7 extends into the inner cavity of the experimental vessel 14 In the middle, the bottom end of the air outlet pipe 9 is connected with a flared gas collection cover, the upper side of the inner cavity of the air outlet pipe 9 is embedded with a dust-proof sponge plug, the current controller 10 is a sliding rheostat current controller, and the heat preservation chamber 12 is made of silicic acid Calcium insulation cavity.
本方案的主要机理为控制变量法，通过不同条件下的同种反应物的变化情况对反应物的生活条件判定，本方案的变量主要为温度、气体的成分及含量、化学试剂；The main mechanism of this program is the control variable method, which determines the living conditions of the reactants through the change of the same reactant under different conditions. The variables of this program are mainly temperature, gas composition and content, and chemical reagents;
四个单元实验室为独立的个体，通过流量控制阀8调控进入相应单元实验室的二氧化碳的浓度，电加热管11采用电阻丝加热方式，电流调控器10与电加热管11串联，通过改变电流调控器10的阻值而改变电加热管11电路上的总阻值，从而改变电加热管11的加热功率；The four unit laboratories are independent individuals, and the concentration of carbon dioxide entering the corresponding unit laboratory is regulated through the flow control valve 8. The electric heating tube 11 adopts a resistance wire heating method, and the current regulator 10 is connected in series with the electric heating tube 11. By changing the current The resistance value of the regulator 10 changes the total resistance value on the circuit of the electric heating tube 11, thereby changing the heating power of the electric heating tube 11;
四个单元实验室从左到右分别为对照组、第一实验组、第二实验组和第三实验组，肿瘤细胞受药后在一定的二氧化碳浓度以及温度条件下进行培养，培养后，将培养后的肿瘤细胞等分为生长状态类似的肿瘤细胞群，分为以下实验方式：The four unit laboratories from left to right are the control group, the first experimental group, the second experimental group and the third experimental group. After receiving the drug, the tumor cells are cultured under certain carbon dioxide concentration and temperature conditions. After culture, the The cultured tumor cells were equally divided into tumor cell groups with similar growth status, which were divided into the following experimental methods:
1、四个单元实验室均不添加化学药剂，对照组中的温度、二氧化碳浓度与原先培养的量保持一致。第一实验组、第二实验组和第三实验组中的温度值相同，且二氧化碳浓度由高到低，第一实验组的二氧化碳浓度高于对照组中的二氧化碳浓度，第三实验组中的二氧化碳浓度低于对照组中的二氧化碳浓度，且二氧化碳的最高浓度与最低浓度之差值为0.6％，培养同样的时间后，通过以上的实验结果画出在温度条件相同的情况下，二氧化碳浓度对肿瘤细胞生长的影响；1. None of the four unit laboratories added chemical agents, and the temperature and carbon dioxide concentration in the control group were kept the same as those previously cultivated. The temperature values in the first experimental group, the second experimental group and the third experimental group are the same, and the carbon dioxide concentration is from high to low, the carbon dioxide concentration in the first experimental group is higher than that in the control group, and the carbon dioxide concentration in the third experimental group The carbon dioxide concentration is lower than the carbon dioxide concentration in the control group, and the difference between the highest concentration and the lowest concentration of carbon dioxide is 0.6%. Effects on tumor cell growth;
2、四个单元实验室均不添加化学药剂，对照组中的温度、二氧化碳浓度与原先培养的量保持一致。第一实验组、第二实验组和第三实验组中的二氧化碳浓度值相同，且温度由高到低，第一实验组的温度高于对照组中的温度，第三实验组中的温度低于对照组中的温度，且温度最大值与最小值之差为10℃，培养同样的时间后，通过以上的实验结果得出温度条件相同的情况下，温度对肿瘤细胞生长的影响；2. None of the four unit laboratories added chemical agents, and the temperature and carbon dioxide concentration in the control group were kept the same as those previously cultivated. The carbon dioxide concentration values in the first experimental group, the second experimental group and the third experimental group are the same, and the temperature is from high to low, the temperature in the first experimental group is higher than that in the control group, and the temperature in the third experimental group is lower The temperature in the control group, and the difference between the maximum value and the minimum value of the temperature is 10°C. After culturing for the same time, the effect of temperature on the growth of tumor cells can be obtained under the same temperature conditions through the above experimental results;
3、对照组、第一实验组、第二实验组和第三实验组温度、二氧化碳浓度与原先培养的量保持一致，对照组中不添加化学药剂，第一实验组、第二实验组和第三实验组中分别注射不同的针对肿瘤细胞的化学药剂，注射量相同，培养同样的时间后观察生长情况。3. The temperature and carbon dioxide concentration of the control group, the first experimental group, the second experimental group and the third experimental group were kept the same as the original cultured amount, no chemical agents were added to the control group, the first experimental group, the second experimental group and the third experimental group The three experimental groups were injected with different chemical agents targeting tumor cells in the same amount, and observed the growth after culturing for the same time.
通过以上三组的控制变量的方式能够判断肿瘤细胞的生长环境以及药物作用对肿瘤细胞的生长的影响。The growth environment of tumor cells and the influence of drug action on the growth of tumor cells can be judged by means of the above three groups of control variables.
尽管已经示出和描述了本发明的实施例，对于本领域的普通技术人员而言，可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型，本发明的范围由所附权利要求及其等同物限定。Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes, modifications and substitutions can be made to these embodiments without departing from the principle and spirit of the present invention. and modifications, the scope of the invention is defined by the appended claims and their equivalents.
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