CN108732229B - Replaceable capillary electrophoresis device - Google Patents
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- CN108732229B CN108732229B CN201810503694.9A CN201810503694A CN108732229B CN 108732229 B CN108732229 B CN 108732229B CN 201810503694 A CN201810503694 A CN 201810503694A CN 108732229 B CN108732229 B CN 108732229B
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- 238000005251 capillar electrophoresis Methods 0.000 title claims abstract description 176
- 238000001514 detection method Methods 0.000 claims abstract description 231
- 239000007853 buffer solution Substances 0.000 claims abstract description 68
- 238000000926 separation method Methods 0.000 claims abstract description 20
- 230000007246 mechanism Effects 0.000 claims description 19
- 239000000126 substance Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 abstract description 18
- 238000012958 reprocessing Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 8
- 230000009471 action Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000004913 activation Effects 0.000 description 3
- 230000005684 electric field Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229910052805 deuterium Inorganic materials 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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Abstract
The embodiment of the application discloses a replaceable capillary electrophoresis device, which comprises a capillary, a fixed structure and a switching structure. The capillary is pre-filled with a separation medium, so that the capillary is in a usable state, the sample can be directly detected without reprocessing, and the capillary is fixedly connected with the switching structure through the fixing structure; the inlet end of the capillary tube is connected to the connecting port of the switching structure, and the outlet end of the capillary tube is positioned in the buffer solution; the switching structure comprises a buffer solution space and a detection sample space, wherein the buffer solution space is communicated with the inlet end through a connecting port when the switching structure is in a first state, and the detection sample space is communicated with the inlet end through the connecting port when the switching structure is in a second state; the switching structure is in a first state prior to detection using the capillary electrophoresis device. After the capillary electrophoresis device completes one-time detection, the used capillary electrophoresis device can be directly replaced by a new capillary electrophoresis device without cleaning, so that the interval time between two detections is shortened.
Description
Technical Field
The application relates to the technical field of detection, in particular to a replaceable capillary electrophoresis device.
Background
Capillary electrophoresis (CAPILLARY ELECTROPHORESIS, CE) technology is a novel liquid phase separation technology using capillary as separation channel and high voltage DC electric field as driving force. The capillary electrophoresis generally adopts a quartz capillary column, and under the action of a high-voltage electric field outside the capillary, a separating medium at one side moves to the negative electrode direction due to positive charge. Meanwhile, in the separation medium, the charged biomolecules move at different speeds in the direction of the electrode opposite to the charged polarity of the charged biomolecules under the action of a high-voltage electric field outside the capillary, so that electrophoresis is formed.
The conventional capillary electrophoresis detection system is internally and fixedly provided with a capillary for detecting a sample, and the type of substances injected into the capillary, such as a buffer solution, a detection sample, a cleaning solution and the like, can be adjusted through a conversion device of the capillary electrophoresis detection system. When detecting a substance contained in a sample in a capillary electrophoresis detection system, the detected sample and the like pollute detection components such as capillaries and sample tanks, and in order to be able to detect the sample next time, the detection components possibly polluted by the previous detection in the capillary electrophoresis detection system need to be thoroughly cleaned. In the cleaning process, various cleaning liquids are sequentially injected into the detection parts such as the capillary tube along with the conversion device, and after the cleaning is finished, a series of treatments such as injection of a separation medium and the like are required to be carried out on the capillary tube again for the next detection.
Therefore, in the capillary electrophoresis detection system, complex procedures of cleaning detection components such as capillaries are required in the two detection processes, and the capillaries are reprocessed to meet the requirement of continuously detecting subsequent samples, so that the interval between the two detection processes is too long, a patient is required to wait for a long time, and the user experience is poor.
Disclosure of Invention
The application solves the technical problem of providing a replaceable capillary electrophoresis device, so that after one-time detection is finished, the used capillary electrophoresis device can be directly replaced by a new capillary electrophoresis device without cleaning, the interval time between two detections is shortened, and the user experience is improved.
Therefore, the technical scheme for solving the technical problems is as follows:
An embodiment of the present application provides an alternative capillary electrophoresis device, comprising: capillary, fixed structure and switching structure:
The capillary is pre-filled with a separation medium, and is fixedly connected with the switching structure through the fixing structure; the inlet end of the capillary tube for receiving the detection sample is connected to the connection port of the switching structure, and the outlet end of the capillary tube is positioned in the buffer solution;
The switching structure comprises a buffer solution space and a detection sample space, wherein the buffer solution space is pre-stored with a buffer solution, the buffer solution space is communicated with the inlet end through the connecting port when the switching structure is in a first state, and the detection sample space is communicated with the inlet end through the connecting port when the switching structure is in a second state; wherein the switching structure is in the first state prior to detection of a detection sample by the capillary electrophoresis device.
Optionally, the switching structure further includes a sample inlet, and the sample inlet is used for injecting a detection sample into the detection sample space; when the capillary electrophoresis device is inserted into the capillary electrophoresis detection system, the sample inlet is positioned outside the capillary electrophoresis detection system.
Optionally, the switching structure comprises a control mechanism, the switching structure is switched between the first state and the second state by triggering the control mechanism, and the control mechanism is located outside the capillary electrophoresis detection system when the capillary electrophoresis device is inserted into the capillary electrophoresis detection system.
Optionally, if the sample is injected into the sample space through the sample inlet, when the capillary electrophoresis detection system detects the sample through the capillary electrophoresis device, the switching structure is switched to the second state so as to inject the sample into the capillary.
Optionally, after the detection sample is injected into the capillary, the switching structure is switched to the first state, so that the detection sample is driven to move in the capillary by using the buffer solution in the buffer solution space.
Optionally, the device further comprises electrodes, wherein the electrodes are arranged at two ends of the capillary, and the electrodes are used for being connected with a power supply in the capillary electrophoresis detection system, so that the power supply can provide voltage to push the detection sample to move in the capillary.
Optionally, the device further comprises a detection window disposed at a proximal outlet end of the capillary tube for detecting a substance in the capillary tube in the detection window through the detection window.
Optionally, the detection window is in a through hole shape or a transparent area on the fixed structure.
Optionally, the fixing structure is card-shaped, and the capillary tube is fixed inside the fixing structure.
Optionally, the capillary tube is U-shaped.
According to the technical scheme, the application has the following beneficial effects:
The application provides a replaceable capillary electrophoresis device, which comprises a capillary, a fixed structure and a switching structure. The switching structure comprises a buffer solution space and a detection sample space, wherein the buffer solution space is pre-stored with a buffer solution, the buffer solution space is communicated with the inlet end through a connecting port when the switching structure is in a first state, and the detection sample space is communicated with the inlet end through the connecting port when the switching structure is in a second state. Before the detection of the detection sample by the device, the switching structure is in the first state, so that the switching structure is connected with the inlet end of the capillary tube for receiving the detection sample, the outlet end of the capillary tube is in the buffer solution, and the inlet end and the outlet end of the capillary tube can be both in the buffer solution, so that the separation medium pre-filled in the capillary tube is hermetically stored, the capillary electrophoresis device can exist independently of the capillary electrophoresis detection system, and in addition, the separation medium is pre-filled in the capillary tube, so that the capillary tube is in a usable state, and the sample can be directly detected without pretreatment, activation and the like. Because the device is provided with the switching structure, and the switching structure is provided with the buffer solution space and the detection sample space, the device can independently realize the switching of the switching structure between the first state and the second state, so that when the capillary electrophoresis device is placed into the capillary electrophoresis detection system for sample detection, as separating media, buffer solution and detection samples are all arranged in the capillary electrophoresis device, when one detection is finished, in order to prevent a new detection sample from being polluted by a previous detection sample or a buffer solution used before, the capillary electrophoresis device is only required to be replaced entirely, and a new capillary electrophoresis device can be directly placed into the capillary electrophoresis detection system for starting the next sample detection for the new detection sample without cleaning the capillary, even the buffer solution space and the detection sample space. Therefore, the capillary electrophoresis device is provided with the capillary, the fixing structure and the switching structure, so that the capillary electrophoresis device can well store the capillary and the separation medium therein when being independent of the capillary electrophoresis detection system, and can switch the buffer solution and the detection sample, so that the capillary electrophoresis device is convenient to reasonably match with the capillary electrophoresis system and realize replaceability, that is, after one detection is finished, the used capillary electrophoresis device can be replaced by a new capillary electrophoresis device directly without cleaning, the interval duration between two detections is shortened, and the user experience is improved.
Drawings
In order to more clearly illustrate the embodiments of the application or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block diagram of an alternative capillary electrophoresis device according to an embodiment of the present application;
FIG. 2 is a block diagram of an alternative capillary electrophoresis device according to an embodiment of the present application;
fig. 3 is a block diagram of a switching structure according to an embodiment of the present application;
Fig. 4 is a block diagram of a switching structure according to an embodiment of the present application;
FIG. 5 is a block diagram of an alternative capillary electrophoresis device according to an embodiment of the present application;
FIG. 6 is a block diagram of an alternative capillary electrophoresis device according to an embodiment of the present application;
fig. 7 is a block diagram of an alternative capillary electrophoresis device according to an embodiment of the present application.
Detailed Description
In order to make the present application better understood by those skilled in the art, the following description will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the accompanying drawings, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The traditional capillary electrophoresis detection system has the defects that the cleaning process is complex, the cleaning time is long, the interval between the two detection is too long, and the user experience is poor; in addition, the cleaning machine used for cleaning the detection member such as the capillary tube is expensive, which results in an excessive cost for detecting the substance contained in the detection sample using the conventional capillary electrophoresis detection system.
Therefore, the embodiment of the application provides a replaceable capillary electrophoresis device, which combines the capillary, the fixed structure, the switching structure and other components to form the capillary electrophoresis device, and the capillary electrophoresis device is independently separated from a capillary electrophoresis detection system to realize the replacement of the capillary electrophoresis device. The device may include a capillary tube, a fixed structure, and a switching structure. Wherein, the capillary is pre-filled with separating medium, and is fixedly connected with the switching structure through the fixing structure; the inlet end of the capillary tube for receiving the detection sample is connected to the connection port of the switching structure, and the outlet end of the capillary tube is positioned in the buffer solution so as to carry out sealing preservation on the capillary tube. The switching structure comprises a buffer solution space and a detection sample space, wherein the buffer solution space is pre-stored with the buffer solution, the buffer solution space is communicated with the inlet end through a connecting port when the switching structure is in a first state, and the detection sample space is communicated with the inlet end through the connecting port when the switching structure is in a second state, so that the switching structure can be switched between the first state and the second state, and corresponding solution is injected into the capillary according to different states of the switching structure, wherein the switching structure is in the first state before the detection sample is detected through the capillary electrophoresis device.
According to the capillary electrophoresis detection device, part of components in the original capillary electrophoresis detection system are improved in a targeted manner and selected for combination, and some components are added together to form the functional entity of the capillary electrophoresis detection device provided by the embodiment of the application, so that the capillary electrophoresis detection device can independently exist outside the capillary electrophoresis detection system and can be independently stored and transported, and meanwhile, the capillary electrophoresis detection device can be simply and conveniently matched with the capillary electrophoresis detection system.
The device provided by the embodiment of the application can well store the capillary and the separation medium therein and can switch the buffer solution and the detection sample when being independent of the capillary electrophoresis detection system; the device is inserted into a capillary electrophoresis detection system, so that a detection sample can be detected by the device. Therefore, when the device is applied to the capillary electrophoresis detection system, the device can be replaced, namely after one detection is finished, the used capillary electrophoresis device can be directly replaced by a new capillary electrophoresis device without cleaning, so that the device can be used in cooperation with the capillary electrophoresis detection device, the interval time between two detections of the electrophoresis detection system can be shortened, the user experience is improved, the structural complexity of the capillary electrophoresis detection system can be greatly simplified, for example, the capillary electrophoresis detection system used in cooperation with the capillary electrophoresis detection device can be used without setting a cleaning component, a component for reprocessing the capillary, and the like, and the manufacturing cost of the capillary electrophoresis detection system is effectively reduced.
It should be noted that the separation medium mentioned in the embodiments of the present application may be various gels, such as polyacrylamide, agarose, dextran, and other gels of different types, and may also be various buffer solutions.
Fig. 1 is a schematic structural diagram of an alternative capillary electrophoresis device according to the present application, which may include: capillary 100, fixed structure 200, and switching structure 300.
In this embodiment, the replaceable capillary electrophoresis device may be inserted into a capillary electrophoresis detection system to detect substances contained in a test sample using the replaceable capillary electrophoresis device.
Wherein, the capillary 100 is pre-filled with a separation medium, and the capillary 100 is fixedly connected with the switching structure 300 through the fixing structure 200; the inlet end of the capillary 100 for receiving the detection sample is connected to the connection port of the switching structure 300, and the outlet end of the capillary 100 is in the buffer solution. Taking fig. 1 as an example, the left end of the capillary tube 100 may be an inlet end, the right end of the capillary tube 100 may be an outlet end, and the outlet end is located in the storage space 400 pre-storing the buffer solution, so that the outlet end is located in the buffer solution, and isolation of the separation medium in the capillary tube from the outside is achieved.
The switching structure 300 may include a buffer solution space 301 in which a buffer solution is pre-stored and a detection sample space 302. The switching structure 300 may include two states, and when the switching structure 300 is in the first state, the buffer solution space 301 communicates with the inlet end of the capillary 100 through the connection port, as shown in fig. 1; when the switching structure 300 is in the second state, the detection sample space 302 communicates with the inlet end of the capillary 100 through the connection port, as shown in fig. 2. The switching structure 300 is in the first state before the detection of the detection sample by the capillary electrophoresis device.
Before the detection of the detection sample by the capillary electrophoresis device, the switching structure 300 is in the first state, that is, the inlet end of the capillary 100 is communicated with the buffer solution space 301 through the connection port, at this time, the inlet end is in the buffer solution, and the outlet end is in the buffer solution, so that the capillary 100 can be sealed and preserved, and the separation medium in the capillary 100 is ensured not to be polluted.
It should be noted that, in the present embodiment, the buffer solution space 301 and the detection sample space 302 in the switching structure 300 may be located at the left side of the detection sample space 302 as shown in fig. 1-2, and of course, the buffer solution space 301 may also be located at the right side of the detection sample space 302.
In addition to the buffer solution space 301 and the detection sample space 302, the switching structure 300 may further comprise other spaces, in which other spaces other substances possibly used in the detection may be stored.
The capillary electrophoresis device provided in this embodiment includes a capillary, a fixing structure, and a switching structure, and the capillary, the fixing structure, and the switching structure have characteristics, so that the capillary electrophoresis device is replaceable and can exist independently of the capillary electrophoresis detection system. When the capillary electrophoresis device is needed to detect a detection sample, the capillary electrophoresis device can be inserted into the capillary electrophoresis detection system, and when the detection is completed, the capillary electrophoresis device can be pulled out of the capillary electrophoresis detection system, and a new capillary electrophoresis device can be replaced, so that the next detection can be immediately performed.
The application provides a replaceable capillary electrophoresis device, which comprises a capillary, a fixed structure and a switching structure. The switching structure comprises a buffer solution space and a detection sample space, wherein the buffer solution space is pre-stored with a buffer solution, the buffer solution space is communicated with the inlet end through a connecting port when the switching structure is in a first state, and the detection sample space is communicated with the inlet end through the connecting port when the switching structure is in a second state. Before the detection of the detection sample by the device, the switching structure is in the first state, so that the switching structure is connected with the inlet end of the capillary tube for receiving the detection sample, the outlet end of the capillary tube is in the buffer solution, and the inlet end and the outlet end of the capillary tube can be both in the buffer solution, so that the separation medium pre-filled in the capillary tube is hermetically preserved, the capillary electrophoresis device can exist independently of the capillary electrophoresis detection system, and the separation medium is pre-filled in the capillary tube, so that the capillary tube is in a usable state without pretreatment, activation and the like. Because the device is provided with the switching structure, and the switching structure is provided with the buffer solution space and the detection sample space, the device can independently realize the switching of the switching structure between the first state and the second state, so that when the capillary electrophoresis device is placed into the capillary electrophoresis detection system for sample detection, as separating media, buffer solution and detection samples are all arranged in the capillary electrophoresis device, when one detection is finished, in order to prevent a new detection sample from being polluted by a previous detection sample or a buffer solution used before, the capillary electrophoresis device is only required to be replaced entirely, and a new capillary electrophoresis device can be directly placed into the capillary electrophoresis detection system for starting the next sample detection for the new detection sample without cleaning the capillary, even the buffer solution space and the detection sample space. Therefore, the capillary electrophoresis device is provided with the capillary, the fixing structure and the switching structure, so that the capillary electrophoresis device can well store the capillary and the separation medium therein when being independent of the capillary electrophoresis detection system, and can switch the buffer solution and the detection sample, so that the capillary electrophoresis device is convenient to reasonably match with the capillary electrophoresis system and realize replaceability, that is, after one detection is finished, the used capillary electrophoresis device can be replaced by a new capillary electrophoresis device directly without cleaning, the interval duration between two detections is shortened, and the user experience is improved.
When the capillary electrophoresis detection system detects a substance contained in a detection sample by using the capillary electrophoresis device, the detection sample needs to be injected into a detection sample space, and then the detection sample is pushed into the capillary by a voltage provided by a power supply of the capillary electrophoresis detection system. In some embodiments, the switching structure 300 further includes a sample inlet 303, as shown in fig. 3. The sample inlet 303 is used to inject the detection sample into the detection sample space 302, and the sample inlet 303 should be aligned with the detection sample space 302.
When the capillary electrophoresis device is inserted into the capillary electrophoresis detection system, the sample inlet 303 may be located outside the capillary electrophoresis detection system for convenience of a user to inject the detection sample into the detection sample space 302 through the sample inlet 303.
When the capillary electrophoresis detection system detects the detection sample through the capillary electrophoresis device, if the detection sample has been injected into the detection sample space 302 through the sample inlet 303, the switching structure 300 is switched to the second state so as to inject the detection sample into the capillary 100.
After the test sample is injected into the capillary 100, the switching structure 300 may be switched to the first state so as to move the test sample in the capillary 100 by using the buffer solution.
To implement switching the switching structure 300 between the first state and the second state, in one implementation, referring to fig. 4, the switching structure 300 further includes a control mechanism 304, where the switching structure 300 may be switched between the first state and the second state by triggering the control mechanism 304, and when the capillary electrophoresis device is inserted into the capillary electrophoresis detection system, the control mechanism 304 may be triggered by a user for convenience, and the control mechanism 304 may be located outside the capillary electrophoresis detection system.
The manner in which the user activates the control mechanism 304 may be that the user presses the control mechanism 304, and the switching structure 300 performs a switching between the first state and the second state every time the user presses.
In this embodiment, whether the detection sample is injected into the capillary 100 or the buffer solution is used to drive the detection sample to move in the capillary 100, the power supply of the capillary electrophoresis detection system may be used, and the power supply may provide a voltage to push the detection sample to move in the capillary 100 or push the detection sample to enter the capillary 100.
The power supply of the capillary electrophoresis detection system can apply voltage to two ends of the capillary 100 on the capillary electrophoresis device through matching with the position of the capillary electrophoresis device, so as to provide voltage for the capillary electrophoresis device, so that a detection sample is injected into the capillary 100 under the action of the voltage, or a buffer solution drives the detection sample to move in the capillary 100 under the action of the voltage.
However, this method requires that the capillary electrophoresis device is inserted into the capillary electrophoresis detection system, and then both ends of the capillary 100 are matched with the position where the voltage is applied by the power supply, and thus the precision of the capillary electrophoresis device and the capillary electrophoresis detection system is required to be high when the capillary electrophoresis device is manufactured.
For this purpose, in one implementation of the present embodiment, electrodes may be provided on the capillary electrophoresis device, and electrodes 500 may be provided at both ends of the capillary 100, as shown in fig. 5, and the electrodes 500 are used to connect to a power supply in the capillary electrophoresis detection system, so that the power supply may provide a voltage to push the detection sample to move in the capillary 100, or push the detection sample into the capillary 100. The power supply of the capillary electrophoresis detection system can be directly connected with the electrodes at the two ends by arranging the electrodes at the two ends of the capillary, thereby providing voltage for the capillary electrophoresis device. Therefore, when the capillary electrophoresis device is manufactured, the matching of the two ends of the capillary on the capillary electrophoresis device and the position of the power supply is not considered too much, the matching precision of the capillary electrophoresis device and the capillary electrophoresis detection system is reduced, the applicability is improved, and the difficulty in manufacturing the capillary electrophoresis device is further reduced.
In this embodiment, the voltage provided by the power source may be different according to the power source. If it is desired to push the test sample to move in the capillary 100, the voltage provided by the power supply may be a first type of voltage; if it is desired to push the test sample into the capillary 100, the voltage provided by the power supply may be of the second type. In some embodiments, the first type of voltage and the second type of voltage may each be high voltages of different voltage ranges. In some embodiments, the second type of voltage may be higher than the first type of voltage.
In some implementations of the present example, referring to fig. 6, the apparatus may further include a detection window 600, the detection window 600 may be disposed at a proximal outlet end of the capillary tube so that the substance in the capillary tube 100 in the detection window 600 is detected through the detection window 600.
Because at near-outlet end, buffer solution can drive the sample to be detected to fully move in the capillary, so that each substance contained in the sample to be detected can be fully separated, and thus, the detection window is arranged at the near-outlet end to detect the sample to be detected, and the detection accuracy can be higher.
Since the substance in the capillary 100 in the detection window 600 is detected through the detection window 600, the capillary 100 is generally irradiated on one side of the capillary electrophoresis device by using detection light, wherein the detection light may be a laser, an LED, a deuterium lamp, or the like; on the other side of the capillary electrophoresis device a detector is used for receiving. Therefore, the detection window 600 should be light transmissive. In one implementation of this embodiment, the detection window may be a through-hole or a transparent region on the fixed structure 200.
The detection window is either through-hole-like or transparent in the fixed structure 200, which may be determined by the material of the fixed structure 200 itself. It will be appreciated that if the fixing structure 200 is made of a non-light-transmitting material, then, in order to detect the substance in the capillary 100 located in the detection window 600, a hole penetrating the fixing structure 200 may be provided in the fixing structure 200, and the penetrating hole may be used as the detection window.
In some cases, since the capillary is fragile and is easily damaged by external force, in order to avoid the capillary from being damaged by external force when contacting with the outside through the through hole, two ends of the through hole may be covered with a transparent material, such as glass, to protect the capillary.
It will be appreciated that if the fixing structure 200 is made of a light-transmitting material, such as glass, the detection window may be a light-transmitting region that remains in the proximal outlet end region of the capillary after the fixing structure 200 is covered with a non-transparent material except for the proximal outlet end region.
In some embodiments, the securing structure 200 may be card-like, and the capillary tube 100 may be secured inside the securing structure 200.
The fixing structure 200 is in a card shape, so that the volume of the capillary electrophoresis device can be reduced, and the capillary electrophoresis device is convenient to carry. Fixing the capillary tube 100 inside the fixing structure 200 can protect the capillary tube 100 from damage due to external force.
In the present embodiment, the capillary tube 100 may be fixed by the fixing structure 100 in an arbitrary shape, such as a linear type. In one implementation of this embodiment, the capillary 100 may be U-shaped in shape. By setting the shape of the capillary 100 to be U-shaped, the area occupied by the capillary on the fixing structure 200 can be reduced, the size of the fixing structure 200 can be reduced, and the size of the capillary electrophoresis device can be further reduced, so that the capillary electrophoresis device is more portable.
Of course, the shape of the capillary 100 may be S-shaped, M-shaped, or the like, and the shape of the capillary 100 is not limited in this embodiment.
Based on the corresponding embodiments of fig. 1-6, the present embodiment provides a capillary electrophoresis device, referring to fig. 7, comprising a capillary 100, a fixing structure 200, a switching structure 300, a storage space 400 pre-storing a buffer solution, an electrode 500, and a detection window 600. Wherein, the capillary 100 is pre-filled with a separation medium, and the capillary 100 is fixedly connected with the switching structure 300 through the fixing structure 200; the inlet end of the capillary 100 for receiving the detection sample is connected to the connection port of the switching structure 300, and the outlet end of the capillary 100 is in the buffer solution in the storage space 400. The switching structure 300 comprises a buffer solution space 301, a detection sample space 302, a sample inlet 303 and a control mechanism 304, wherein the control mechanism 304 is a key. Electrodes 500 are disposed at both ends of the capillary 100. A detection window 600 is provided near the outlet end of the capillary tube 100.
The switching structure 300 is in a first state prior to detection of a detection sample by the capillary electrophoresis device. When the capillary electrophoresis device is required to detect a detection sample, the capillary electrophoresis device can be inserted into a capillary electrophoresis detection system, and the electrode 500 of the capillary electrophoresis device can be connected with a power supply of the capillary electrophoresis detection system. Then, the control mechanism 304 is pressed to switch the switching structure 300 to the second state, at this time, the detection sample may be injected into the detection sample space 302 through the sample inlet 303, and the detection sample is pushed into the capillary 100 by using a voltage provided by the power supply, where the voltage may be the second type of voltage. Then, the control mechanism 304 is pressed to switch the switching structure 300 to the first state, and the buffer solution drives the detection sample to move in the capillary 100 by using the voltage provided by the power supply, wherein the voltage may be the first type of voltage. The capillary electrophoresis detection system detects the detection sample in the capillary 100 at the detection window 600 to obtain the substance contained in the detection sample. When the detection is completed, the capillary electrophoresis device can be pulled out from the capillary electrophoresis detection system, and a new capillary electrophoresis device can be inserted to immediately perform the next detection.
Therefore, the capillary electrophoresis device has the capillary, the fixed structure and the switching structure, so that the capillary electrophoresis device can well store the capillary and the separation medium therein when being independent of the capillary electrophoresis detection system, and can switch the buffer solution and the detection sample, so that the capillary electrophoresis device is convenient to reasonably match with the capillary electrophoresis system, and the replaceability is realized; in addition, the capillary is pre-filled with the separation medium, so that the capillary can be in a usable state, and pretreatment, activation and the like are not needed. That is, after one detection is completed, the used capillary electrophoresis device can be directly replaced by a new capillary electrophoresis device, cleaning is not needed, the interval time between two detections is shortened, and the user experience is improved.
The foregoing is merely a preferred embodiment of the present application and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present application, which are intended to be comprehended within the scope of the present application.
Claims (8)
1. A replaceable capillary electrophoresis device comprising a capillary tube, a fixed structure, and a switching structure:
The capillary tube is filled with a separation medium in advance so that the capillary tube is in a usable state, and the capillary tube is fixedly connected with the switching structure through the fixing structure; the inlet end of the capillary tube for receiving the detection sample is connected to the connection port of the switching structure, and the outlet end of the capillary tube is positioned in the buffer solution;
The switching structure comprises a buffer solution space and a detection sample space, wherein the buffer solution space is pre-stored with a buffer solution, the buffer solution space is communicated with the inlet end through the connecting port when the switching structure is in a first state, and the detection sample space is communicated with the inlet end through the connecting port when the switching structure is in a second state; wherein the switching structure is in the first state prior to detection of a detection sample by the capillary electrophoresis device; after detecting the detection sample by the capillary electrophoresis device, pulling out the capillary electrophoresis device from the capillary electrophoresis detection system, and replacing a new capillary electrophoresis device to immediately perform the next detection;
the switching structure further comprises a sample inlet, wherein the sample inlet is used for injecting a detection sample into the detection sample space; when the capillary electrophoresis device is inserted into a capillary electrophoresis detection system, the sample inlet is positioned outside the capillary electrophoresis detection system;
The switching structure comprises a control mechanism, the switching structure is switched between the first state and the second state by triggering the control mechanism, the control mechanism is positioned outside the capillary electrophoresis detection system when the capillary electrophoresis device is inserted into the capillary electrophoresis detection system, and the process of switching the switching structure between the first state and the second state by triggering the control mechanism comprises the following steps: the switching structure switches between the first state and the second state once every time the control mechanism is pressed.
2. The apparatus of claim 1, wherein the switching mechanism is switched to the second state for injecting the test sample into the capillary when the capillary electrophoresis detection system detects the test sample by the capillary electrophoresis device if the test sample is injected into the test sample space through the sample inlet.
3. The device of claim 2, wherein the switching structure is switched to the first state after the detection sample is injected into the capillary tube so as to move the detection sample in the capillary tube by using the buffer solution in the buffer solution space.
4. The device of claim 1, further comprising electrodes disposed at both ends of the capillary, the electrodes for connection to a power source in the capillary electrophoresis detection system such that the power source provides a voltage to push the detection sample to move in the capillary.
5. The device of claim 1, further comprising a detection window disposed at a proximal outlet end of the capillary tube for detecting substances in the capillary tube in the detection window through the detection window.
6. The device of claim 5, wherein the detection window is a through-hole or a transparent region on the fixed structure.
7. The device of claim 1, wherein the securing structure is card-like and the capillary tube is secured within the securing structure.
8. The device of claim 1, wherein the capillary tube is U-shaped in shape.
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| DE202005009960U1 (en) * | 2005-06-24 | 2005-09-01 | Forschungszentrum Karlsruhe Gmbh | Measurement unit, for capillary electrophoresis, has a capillary array in a micro-fluid chip within a box with conductive walls, a high tension supply and a high frequency transmitter/receiver for non-contact conductivity detection |
| JP4825731B2 (en) * | 2007-06-01 | 2011-11-30 | 株式会社日立ハイテクノロジーズ | Capillary electrophoresis device |
| JP2012058039A (en) * | 2010-09-07 | 2012-03-22 | Arkray Inc | Analytical method of sample by capillary electrophoretic method, capillary electrophoretic apparatus and chip for capillary electrophoresis |
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