CN108844855A - A kind of spore granule density and partial size monitoring analysis system - Google Patents
A kind of spore granule density and partial size monitoring analysis system Download PDFInfo
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- CN108844855A CN108844855A CN201810324021.7A CN201810324021A CN108844855A CN 108844855 A CN108844855 A CN 108844855A CN 201810324021 A CN201810324021 A CN 201810324021A CN 108844855 A CN108844855 A CN 108844855A
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- collecting chamber
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- feeding pipe
- light collecting
- branch pipe
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- 239000008187 granular material Substances 0.000 title claims abstract description 18
- 238000004458 analytical method Methods 0.000 title claims abstract description 15
- 238000012544 monitoring process Methods 0.000 title claims abstract description 10
- 239000004065 semiconductor Substances 0.000 claims description 7
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000005192 partition Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 13
- 241000233866 Fungi Species 0.000 abstract description 12
- 238000001514 detection method Methods 0.000 abstract description 4
- 230000002860 competitive effect Effects 0.000 abstract description 3
- 239000000356 contaminant Substances 0.000 abstract description 2
- 239000002245 particle Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 230000002902 bimodal effect Effects 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 241000221960 Neurospora Species 0.000 description 1
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
- 241000207961 Sesamum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N9/00—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity
- G01N9/24—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity by observing the transmission of wave or particle radiation through the material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
- G01N15/0211—Investigating a scatter or diffraction pattern
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
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- Microbiology (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
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- Dispersion Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A kind of spore granule density and partial size monitoring analysis system, including sample feeding pipe are equipped with inner tube in the sample feeding pipe, and sample introduction bottom of the tube be connected to scattering light collecting chamber, is equipped with ellipsoid in scattering light collecting chamber and is focused border, scatters light collecting chamber two sides equipped with opposite opening；The sample feeding pipe side is equipped with outer tube, and there are two filters and sheath stream to pump for dress on outer tube, and filter rear portion outer tube is parallel with branch pipe one and branch pipe two, and branch pipe one is equipped with sheath flow pressure sensor and total flow pressure sensor；Two lower end of branch pipe is connected to sample feeding pipe lower part respectively, branch pipe one is connected to, and the outlet of sample feeding pipe tail portion is equipped with filter and total stream pump；Advantages of the present invention is using spore granule density and granularmetric analysis and quickly; it is detected to air miospore granule density during scale Edible Fungi and Size; fit the granule density of contained miscellaneous bacteria spore in total spore count in air; quickly analysis Edible Fungi environment is realized the detection that Edible Fungi is coerced by competitive miscellaneous bacteria by living contaminants security level.
Description
Technical field
The present invention relates to field of biotechnology, more particularly, to a kind of spore granule density and partial size monitoring analysis system.
Background technique
China is one of the country for recognizing in the world and utilizing edible mushroom earliest, B.C. 475 ~ 221 year the Warring States Period
《Column》One book just has the record of " on rotten earth, there is bacterium sesame person "；In recent years, edible fungus industrial large-scale production is emerging in China
It rises, edible mushroom yield is with the speed increase in 30%/year, and by 2013, the total output of China edible mushroom accounted for the 65% of Gross World Product
More than, it ranks first in the world.Exactly because the extension of production-scale continuous expansion and planting time, edible fungus damage increase simultaneously year by year
Aggravate, every year because of disease to mushroom grower caused by largely lose, seriously restrict edible fungus industrial production development.Cause disease
The main reason for be to be infected by competitive miscellaneous bacteria during Edible Fungi（Including Neurospora, aspergillus, mould, Paecilomyces varioti, hair
Mould, head mold, trichoderma and bacterium class）；These are very big to Influence of production, gently the then underproduction, heavy then have no harvest, and seriously affect the yield of fresh mushroom
And quality；Polluting main cause is：1. raw material is stale, sterilizing is not thorough in addition, and miscellaneous bacteria spreads rapidly in the short time；2. strain
Miscellaneous bacteria is carried, is mainly shown as around strain after inoculation and infects miscellaneous bacteria, 3. miscellaneous bacteria radix is big in culturing room, in cultivating in a fungus bag mistake
Cheng Zhong, miscellaneous bacteria spore flow into bacterium bag with air, pollute.For example horizontal slide method of currently used Detection method,
Serial dilution, the vertical or inclination microbe colonies cultivation such as slide method or vertical cylindrical body method need 2~3 day time；And
The spore seizing devices methods such as constant volume type spore seizing device method, mobile spore seizing device method need a few hours by 1 day, it is also necessary to people
Microscopically observation is carried out to the microorganism on pull-in range.Not only it will take a lot of manpower and time for these methods, but also
Can not field quick detection, be far from satisfying the demand of actual production.
Summary of the invention
In order to overcome the problems referred above, the technical solution adopted by the present invention is as follows：
A kind of spore granule density and partial size monitoring analysis system, including sample feeding pipe are equipped with inner tube, sample feeding pipe in the sample feeding pipe
Partition is equipped between inner tube, sample introduction bottom of the tube is connected to scattering light collecting chamber, and ellipsoid, which is equipped with, in scattering light collecting chamber focuses border,
It scatters light collecting chamber two sides and is equipped with opposite opening, scattering light collecting chamber side is equipped with semiconductor laser and light beam formation group
Part, the scattering light collecting chamber other side are equipped with optical beam dump, and opening, semiconductor laser, light beam form component and beam collection
Device is located along the same line；The sample feeding pipe side is equipped with outer tube, and there are two filters and sheath stream to pump for dress on outer tube, after filter
Portion's outer tube is parallel with branch pipe one and branch pipe two, and branch pipe one is equipped with sheath flow pressure sensor and total flow pressure sensor；Branch pipe two
Lower end is connected to sample feeding pipe lower part respectively, branch pipe one is connected to；Branch pipe one is connected to the sample feeding pipe by scattering light collecting chamber to be converged,
The outlet of sample feeding pipe tail portion is equipped with filter and total stream pump.
Preferably, the inner tube in the sample feeding pipe enters scattering light collecting chamber through partition.
Preferably, said inner tube end forms nozzle in scattering light collecting chamber, accelerates sample flowing velocity.
Preferably, the quantity of the filter is two, and sheath stream pump is arranged between two filters.
Advantages of the present invention：Using spore granule density and granularmetric analysis and quickly, to scale Edible Fungi process
Middle air miospore granule density and Size detection, fit the granule density of contained miscellaneous bacteria spore in total spore count in air, fastly
Speed analysis Edible Fungi environment is realized the inspection that Edible Fungi is coerced by competitive miscellaneous bacteria by living contaminants security level
It surveys.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of invention.
Fig. 2 ellipsoid of the present invention focuses the structural schematic diagram in border.
Specific embodiment
As shown in Figure 1 and Figure 2, a kind of spore granule density and partial size monitoring analysis system, including sample feeding pipe 1, the sample introduction
It is equipped with inner tube 2 in pipe 1, partition is equipped between sample feeding pipe 1 and inner tube 2,1 bottom of sample feeding pipe is connected to scattering light collecting chamber 3, is scattered
Be equipped with ellipsoid in light collecting chamber 3 and focus border 4, ellipsoid focuses border 4 and is equipped with photomultiplier tube, measure the bimodal signal of scattering and by
Electronics processing system completes real-time particle flight time measurement and particle counting, and scattering 3 two sides of light collecting chamber are equipped with opposite
Opening 5, scattering 3 side of light collecting chamber is equipped with semiconductor laser 6 and light beam forms component 7, and scattering 3 other side of light collecting chamber is set
There is optical beam dump 8, opening 5, semiconductor laser 6, light beam form component 7 and optical beam dump 8 is located along the same line；Institute
It states 1 side of sample feeding pipe and is equipped with outer tube 9, dress is inhaled in inner tube 2 there are two filter 10 and sheath stream pump 11 for filtering, pumping on outer tube 9
Sample, 10 rear portion outer tube 9 of filter is parallel with branch pipe 1 and branch pipe 2 13, and branch pipe 1 is equipped with sheath flow pressure sensor
14 and total flow pressure sensor 15, for measuring pressure；2 13 lower end of branch pipe is connected to 1 lower part of sample feeding pipe respectively, branch pipe 1
Connection；Branch pipe 1 be connected to and converges with the sample feeding pipe 1 by scattering light collecting chamber 3, the outlet of 1 tail portion of sample feeding pipe equipped with filter with
Total stream pump, total stream pump extract two strands of samples from scattering light collecting chamber 3 out.
Inner tube 2 in sample feeding pipe 1 enters scattering light collecting chamber 3 through partition, is used to form two strands of sample flows.
2 end of inner tube forms nozzle in scattering light collecting chamber 3, accelerates sample flowing velocity.
The quantity of filter 10 is two, and sheath stream pump 11 is arranged between two filters 10, ensure that filter effect.
Working principle：
A part of sample flow enters the scattering progress particle flight time measurement of light collecting chamber 3 by nozzle by inner tube 1, and in addition one
Part sample then passes through filter 10 and sheath stream pump 11 is flowed through from outer tube 9, and two strands of samples are taken out through total stream pump from scattering light collecting chamber 3
Out；When single spore particle is by nozzle accelerating region, into measurement zone, spore particle passes through semiconductor laser 6 and light beam shape
At the scattering light that component 7 generates, opening 5 is scattered light through, 5 other sides of opening are equipped with optical beam dump 8, focus border by ellipsoid
4 collect scattering light and converge to photomultiplier tube, measure the bimodal signal of scattering and complete real-time grain by electronics processing system
Sub- flight time measurement and particle counting；Spore scattered light signal photoelectric conversion, flight time are realized using electron controls technology
Precise measurement, spore are by the functions such as particle size classification storage and the storage of spore number size distribution；By spore granule density and
The Particle density distribution inversion of granularmetric analysis algorithm completion different-grain diameter fine particle.According to spore granule density and the scattered signal of partial size
Bimodal interval and air-flow velocity etc. obtain particle diameter distribution；Since contained various spores are micro- lifes in the air of Edible Fungi environment
Object living body obtains the overall particle size of the gross density of various spores and various partial size spores in air and composes.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Within the technical scope disclosed by the invention, any changes or substitutions that can be easily thought of by those skilled in the art, should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be to protect model defined by claims
Subject to enclosing.
Claims (4)
1. a kind of spore granule density and partial size monitoring analysis system, including sample feeding pipe（1）, it is characterised in that：The sample feeding pipe
（1）It is interior to be equipped with inner tube（2）, sample feeding pipe（1）With inner tube（2）Between be equipped with partition, sample feeding pipe（1）Bottom and scattering light collecting chamber
（3）Connection scatters light collecting chamber（3）The interior ellipsoid that is equipped with focuses border（4）, scatter light collecting chamber（3）Two sides are equipped with opposite opening
（5）, scatter light collecting chamber（3）Side is equipped with semiconductor laser（6）Component is formed with light beam（7）, scatter light collecting chamber（3）Separately
Side is equipped with optical beam dump（8）, opening（5）, semiconductor laser（6）, light beam formed component（7）And optical beam dump（8）
It is located along the same line；The sample feeding pipe（1）Side is equipped with outer tube（9）, outer tube（9）There are two filters for upper dress（10）And sheath stream
Pump（11）, filter（10）Rear portion outer tube（9）It is parallel with branch pipe one（12）With branch pipe two（13）, branch pipe one（12）It is equipped with sheath stream
Pressure sensor（14）And total flow pressure sensor（15）；Branch pipe two（13）Lower end respectively with sample feeding pipe（1）Lower part connection, branch pipe
One（12）Connection；Branch pipe one（12）Light collecting chamber is scattered with passing through（3）Sample feeding pipe（1）Connection converges, sample feeding pipe（1）Tail portion goes out
Mouth is pumped equipped with filter and total stream.
2. a kind of spore granule density according to claim 1 and partial size monitoring analysis system, it is characterised in that：The sample introduction
Pipe（1）Interior inner tube（2）Enter scattering light collecting chamber through partition（3）.
3. a kind of spore granule density according to claim 1 and partial size monitoring analysis system, it is characterised in that：Said inner tube
（2）End is in scattering light collecting chamber（3）Form nozzle.
4. a kind of spore granule density according to claim 1 and partial size monitoring analysis system, it is characterised in that：The filtering
Device（10）Quantity be two, sheath stream pump（11）It is arranged in two filters（10）Between.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810324021.7A CN108844855B (en) | 2018-04-12 | 2018-04-12 | Spore particle density and particle size monitoring and analyzing system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810324021.7A CN108844855B (en) | 2018-04-12 | 2018-04-12 | Spore particle density and particle size monitoring and analyzing system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108844855A true CN108844855A (en) | 2018-11-20 |
| CN108844855B CN108844855B (en) | 2021-07-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810324021.7A Expired - Fee Related CN108844855B (en) | 2018-04-12 | 2018-04-12 | Spore particle density and particle size monitoring and analyzing system |
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| Country | Link |
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| CN (1) | CN108844855B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111413258A (en) * | 2020-04-28 | 2020-07-14 | 重庆飞扬测控技术研究院有限公司 | A kind of aerosol particle size spectrometer analysis device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1979127A (en) * | 2006-12-18 | 2007-06-13 | 中国矿业大学(北京) | Improved method for quick detection of Ramaria root spore density |
| CN102507508B (en) * | 2011-09-28 | 2014-03-12 | 天津大学 | Flow measurement system for detecting tumor cells and analysis and monitoring method |
| US8773661B2 (en) * | 2009-01-23 | 2014-07-08 | University Of Washington | Virtual core flow cytometry |
| WO2015124942A2 (en) * | 2014-02-20 | 2015-08-27 | Malvern Instruments Limited | Heterogeneous fluid sample characterization |
| CN105008895A (en) * | 2012-10-15 | 2015-10-28 | 纳诺赛莱克特生物医药股份有限公司 | Systems, devices and methods for particle sorting |
| CN105181535A (en) * | 2015-09-15 | 2015-12-23 | 上海友助机械有限公司 | Aerosol particle size screening equipment |
-
2018
- 2018-04-12 CN CN201810324021.7A patent/CN108844855B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1979127A (en) * | 2006-12-18 | 2007-06-13 | 中国矿业大学(北京) | Improved method for quick detection of Ramaria root spore density |
| US8773661B2 (en) * | 2009-01-23 | 2014-07-08 | University Of Washington | Virtual core flow cytometry |
| CN102507508B (en) * | 2011-09-28 | 2014-03-12 | 天津大学 | Flow measurement system for detecting tumor cells and analysis and monitoring method |
| CN105008895A (en) * | 2012-10-15 | 2015-10-28 | 纳诺赛莱克特生物医药股份有限公司 | Systems, devices and methods for particle sorting |
| WO2015124942A2 (en) * | 2014-02-20 | 2015-08-27 | Malvern Instruments Limited | Heterogeneous fluid sample characterization |
| CN105181535A (en) * | 2015-09-15 | 2015-12-23 | 上海友助机械有限公司 | Aerosol particle size screening equipment |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111413258A (en) * | 2020-04-28 | 2020-07-14 | 重庆飞扬测控技术研究院有限公司 | A kind of aerosol particle size spectrometer analysis device |
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| CN108844855B (en) | 2021-07-13 |
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