CN108858309B - Cutting device of pathogen offset coated by pulsed field gel electrophoresis - Google Patents
Cutting device of pathogen offset coated by pulsed field gel electrophoresis Download PDFInfo
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- CN108858309B CN108858309B CN201810645313.0A CN201810645313A CN108858309B CN 108858309 B CN108858309 B CN 108858309B CN 201810645313 A CN201810645313 A CN 201810645313A CN 108858309 B CN108858309 B CN 108858309B
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- gel
- glue
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- cutting
- piece
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- 230000001717 pathogenic effect Effects 0.000 title claims abstract description 19
- 244000052769 pathogen Species 0.000 title claims abstract description 18
- 238000003906 pulsed field gel electrophoresis Methods 0.000 title claims abstract description 16
- 239000003292 glue Substances 0.000 claims abstract description 96
- 238000001914 filtration Methods 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000005192 partition Methods 0.000 claims abstract description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 27
- 238000003825 pressing Methods 0.000 claims description 22
- 229910052742 iron Inorganic materials 0.000 claims description 13
- 239000000872 buffer Substances 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 210000001503 joint Anatomy 0.000 claims 1
- 239000007984 Tris EDTA buffer Substances 0.000 abstract description 9
- 239000000243 solution Substances 0.000 abstract description 5
- 230000000712 assembly Effects 0.000 abstract description 2
- 238000000429 assembly Methods 0.000 abstract description 2
- 239000000853 adhesive Substances 0.000 description 5
- 230000001070 adhesive effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 239000004836 Glue Stick Substances 0.000 description 3
- 239000002390 adhesive tape Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D1/00—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor
- B26D1/01—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work
- B26D1/04—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work having a linearly-movable cutting member
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/01—Means for holding or positioning work
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/20—Cutting beds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/26—Means for mounting or adjusting the cutting member; Means for adjusting the stroke of the cutting member
- B26D7/2614—Means for mounting the cutting member
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/27—Means for performing other operations combined with cutting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Coating Apparatus (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a device for cutting a pathogen gel block coated by pulsed field gel electrophoresis, which comprises a gel cutting platform, a cutter head and a gel carrying piece, wherein the cutter head is provided with blade assemblies with a distance of 2mm, manual alignment is not needed, and the accuracy of gel cutting is ensured; the glue cutting platform is provided with a water filtering structure, so that the glue blocks to be cut and TE buffer solution can be conveniently separated and recycled, the glue carrying piece is matched with the glue cutting platform for use, the cut small glue blocks are conveniently taken out, and the glue carrying piece and the glue cutting platform are further led into the boss of the partition to form a plurality of independent glue cutting areas, so that a plurality of glue blocks can be cut simultaneously, and the glue cutting platform is efficient and rapid. The invention thoroughly changes the current situation that the error of the current manual cutting glue block is large and the speed is low.
Description
Technical Field
The invention belongs to the technical field of pathogenic biology, and particularly relates to a gel cutting device for a pulse field gel electrophoresis coated pathogen gel block.
Background
At present, when the pulse field gel electrophoresis experiments are carried out in pathogen molecular typing laboratories of disease prevention and control centers in China and various levels according to standard operation procedures, small gel blocks embedded with pathogen are required to be cleaned and then cut into adhesive tapes with the width of 2mm so as to facilitate the next enzyme cutting. The existing experimental technology is that glue blocks and TE buffer solution in a preservation pipe are poured onto a plate, the plate is rocked to separate the glue blocks from the TE buffer solution, then the TE buffer solution is poured back into the preservation pipe for recycling, only pathogenic glue blocks are reserved on the plate, an experimental operator uses a blade to cut off glue strips about 2mm wide, the glue strips are transferred into an EP pipe, and the residual glue blocks are put back into the preservation pipe.
The method has the following disadvantages: 1. the whole process is required to be operated one by one, which is tedious and time-consuming, has higher requirements on the technical level and the proficiency of experimental operators, and has large workload especially when the sample size is large, and the experimental operators are easy to fatigue operation, so that the glue cutting effect is poor; 2. the equipment required by the experiment is self-made, and each laboratory has no unified standard; 3. the standard subjectivity of the width of the cut adhesive tape is too strong, the width of the cut adhesive tape is different from person to person, the difference is large, and the electrophoresis separation effect is affected.
Chinese patent CN201724854U discloses a glue cutting die, which controls the width of glue cut by drawing a mark line, but it is necessary to manually align the glue block with the mark line, and if the mark line is not aligned or slipping occurs during cutting, the glue strip with accurate width cannot be obtained.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide a gel cutting device for coating pathogen gel blocks by pulse field gel electrophoresis. The device need not artificial correction width, guarantees the accuracy of size and the stability of cutting, can cut a plurality of glue pieces simultaneously moreover, has improved surely gluey efficiency greatly.
The technical scheme adopted by the invention is as follows:
The utility model provides a cut gluey device of pulse field gel electrophoresis coating pathogen offset, its characterized in that includes cuts gluey platform, tool bit and carries gluey piece, cut gluey platform and include the offset place platform 7 that has base recess 6, set up the tool bit stopper 5 that has spacing groove 4 on the offset place platform 7; the cutter head comprises a pressing piece 9 and a blade assembly 1 fixedly connected with the bottom of the pressing piece 9 through a fixed iron piece 10, wherein the blade assembly 1 consists of 2 parallel blades with the interval of 2mm, the cutter head also comprises a cutter head seat 8, the cutter head seat 8 is sleeved outside the fixed iron piece 10 through a clamping groove 21 penetrating up and down, a first spring is arranged between the pressing piece 9 and the cutter head seat 8, and the fixed iron piece 10 and the blade assembly 1 can move up and down in the clamping groove relative to the cutter head seat 8 through compressing the first spring; the tool bit seat 8 is also provided with a limit post 2 matched with the limit groove 4; the glue carrying piece comprises a handle 11 and a glue carrying rod 12 fixedly connected with the handle, the glue carrying rod 12 extends into the base groove 6 from one side, and the top end of the glue carrying rod is in the same plane with the glue block placing platform 7 and corresponds to the blade assembly 1; when cutting glue, the glue block is placed on the glue block placing platform 7, the limiting column 2 is clamped into the limiting groove 4, the pressing piece 9 is pressed, the first spring is compressed, the blade assembly 1 stretches out of the bottom of the tool bit seat 8 to cut the glue block, the cut glue tape with the width of 2mm is left on the glue carrying rod 12, and the glue carrying piece is pulled out to obtain the glue tape with the width of 2 mm.
In one embodiment of the present invention, two cutter head limiting blocks 5 are located at two ends of the base groove 6, and one end of the base groove 6 penetrates through the cutter head limiting blocks 5 on the same side, so that the glue carrying rod 12 extends into the two cutter head limiting blocks. In order to strengthen the structure and prolong the service life and reduce friction, the clamping groove 21 is constructed by iron pieces, and other structures of the tool bit seat can be made of plastics.
The glue cutting device ensures the glue cutting width through a group of blade assemblies, does not need to be compared with a width marking line, and greatly reduces human errors.
As a preferable scheme, the bottom of the glue carrying rod 12 is connected with the glue carrying base 19 to form an integral structure with a 'convex' shape in cross section, and the cavity of the base groove 6 is matched with the 'convex' integral structure. The arrangement of this structure provides a stable base for the glue stick 12 and further secures the glue stick 12 together with the recess 6, avoiding sliding of the glue stick 12.
As the preferable scheme, the thickness of the glue carrying rod 12 is more than 0mm and less than 2mm, the glue carrying rod 12 and the glue block placing platform 7 are respectively divided into a plurality of glue cutting areas by a plurality of first bosses 3 and a plurality of second bosses 13, and the first bosses 3 correspond to the second bosses 13. The boss design enables the glue cutting device to realize the function of simultaneously cutting a plurality of glue.
As a more preferable scheme, the glue carrying rod 12 and the glue block placing platform 7 are divided into 4-6 glue cutting areas.
As a further preferable scheme, the protruding height of the first boss 3 and the second boss 13 is 1mm, and the thickness of the glue carrying rod 12 is 1.5mm.
As a preferable scheme, the outer side of the bottom of the pressing piece 9 is provided with a protruding piece 15, the cutter head cover 14 is sleeved outside the pressing piece 9, the cover top is clamped on the protruding piece 15, the cutter head cover 14 is fixedly connected with the cutter head seat 8, and when the pressing piece 9 is not pressed, the first spring is in a free state. The design of the cutter head cover covers the connecting structure of the first spring on one hand, so that the glue cutting device is more attractive, and on the other hand, when the first spring rebounds from compression, the control pressing piece 9 does not pop out of the top of the cutter head cover, and the phenomenon that the pressing piece 9 cannot accurately and rapidly penetrate into the cutter head cover when being pressed again is avoided.
As a preferable scheme, the bottom of the cutter head seat 8 is fixedly connected with the rubber block placing platform 7 through a second spring. And the second spring keeps an inherent gap between the cutter head and the rubber block placing platform 7 in a free state, so that the rubber block is conveniently plugged in, and the cutter head does not need to be taken down.
As the preferable scheme, cut gluey platform still includes through buffer platform 16 and gluey piece place the filtration inclined plane 17 that platform 7 is connected, has a plurality of filtration pore on the filtration inclined plane 17, has the basin under the inclined plane, the external apopore 18 of basin.
Preferably, the filtering inclined plane 17 and the water tank under the inclined plane are divided into a plurality of water filtering areas by a plurality of partitions 20, and each area corresponds to one water outlet hole 18.
Preferably, the filtering inclined surface 17 and the water tank positioned under the inclined surface are divided into 4-6 filtering areas.
The term "several" as used herein refers to 1 or more than 1, selected and designed according to the actual needs.
When the glue block storage device is used, glue blocks and TE buffer liquid in a plurality of storage pipes are respectively poured into a water filtering area directly, the TE buffer liquid is recycled from a water outlet, the glue blocks are respectively placed on a glue block placing platform to be cut, and then a plurality of cut small glue blocks are simultaneously taken down from a glue carrying rod, so that the glue block storage device is simple, quick and accurate.
Drawings
FIG. 1 is a diagram of a glue cutting platform of a glue cutting device according to an embodiment of the invention;
FIG. 2 is a block diagram of a button-fixed iron-cutter assembly of a cutter head portion of the glue cutting apparatus of the present invention;
FIG. 3 is a front view of the structure of a cutter head cover of the glue cutting device of the invention;
FIG. 4 is a bottom view of the structure of FIG. 3;
FIG. 5 is a view showing the structure of a cutter head seat of the glue cutting device of the invention;
FIG. 6 is a bottom view of the structure of FIG. 5;
FIG. 7 is a block diagram of a glue carrier of the glue cutting apparatus of the present invention;
the cutter blade comprises a cutter blade assembly 1, a limiting column 2, a first boss 3, a limiting groove 4, a cutter head limiting block 5, a groove 6, a rubber block placing platform 7, a cutter head seat 8, a pressing piece 9, a fixed iron piece 10, a handle 11, a rubber carrying rod 12, a second boss 13, a cutter head cover 14, a protruding piece 15, a buffering platform 16, a filtering inclined plane 17, a water outlet hole 18, a rubber carrying base 19, a partition 20 and a clamping groove 21.
Detailed Description
The following are only preferred embodiments of the present invention, and the scope of the above-mentioned subject matter should not be construed as limited to the following examples, and the various features of the present invention can be arbitrarily combined without contradiction, which are all within the scope of the present invention.
Examples
See fig. 1-7.
Fig. 1 is a block diagram of a preferred glue cutting platform of a glue cutting device, the platform is provided with a glue block placing platform 7, and the glue block placing platform 7 is longitudinally provided with a base groove 6. Two ends of the rubber block placing platform 7 are provided with cutter head limiting blocks 5 with limiting grooves 4, and the limiting grooves 4 are used for being matched with limiting structures of cutter heads to guide cutting positions of the cutter heads; the base groove 6 penetrates through the cutter head limiting block 5 on one side. The purpose of the penetration is to facilitate the insertion of the glue carrier rod 12 of the glue carrier therein. The glue block placing platform 7 is also evenly divided by a plurality of second bosses with the height of 1mm, and the width is not smaller than the width of the glue block to be cut. Connected with the rubber block placing platform 7 is a buffer platform 16, the other side of the buffer platform 16 is connected with a filtering inclined plane 17, the filtering inclined plane 17 is provided with a plurality of filtering holes, a water tank is arranged right below the inclined plane, and the water tank is externally connected with a water outlet hole 18. The filtering inclined surface 17 and the corresponding water tank are separated into a plurality of water filtering areas by a plurality of partitions 20, and each area can independently separate and filter the gel block to be cut and the TE buffer. The cavity of the base groove 6 is matched with the glue carrying rod of the glue carrying piece in shape.
Fig. 2 shows a part of the structure of the cutter head, and the pressing piece 9, the fixed iron piece 10 and the blade assembly 1 are fixedly connected from top to bottom. The blade assembly 1 consists of two parallel aligned and indirectly 2mm blades. The structure can move up and down relative to the cutter head cover and the cutter head seat along with the compression of the spring so as to realize the cutting of the rubber block. The outer side of the bottom of the push piece 9 is provided with a projection 15 which is intended to be snapped into the head cover and which limits the detachment of the part of the structure to the top from the head cover.
Fig. 3 and 4 show the head cover 14 of the head, which has a hollow structure for the push-piece 9 to pass through and a cover top for the catching projection 15. The bottom of the cutter head cover is provided with a bolt hole connected with the cutter head seat through a bolt.
Fig. 5 and 6 show the structure of the tool bit holder 8, in which a clamping groove 21 surrounded by iron pieces is provided in the middle vertical direction, and the clamping groove 21 mainly limits and supports the up-and-down movement of the fixed iron piece 10 and the blade assembly 1. The other parts of the tool bit seat 8 are of plastic structures fixedly connected with the iron parts, and bolt holes are also arranged at positions corresponding to the bolt holes of the tool bit cover. And two ends of the tool bit seat 8 are provided with limiting columns 2, and the limiting columns 2 are inserted into the limiting grooves 4 to enable the tool bit to be accurately abutted with the rubber cutting platform. The upper end of the tool bit holder 8 is fixedly connected with the pressing piece 9 by a spring, preferably with a protruding piece 15 of the pressing piece 9. The pressing piece 9 is pressed, the whole structure shown in fig. 2 moves downwards relative to the cutter head cover 14 and the cutter head seat 8, and the cutter blade assembly 1 extends out of the bottom of the cutter head seat 8 to cut the rubber block. According to the requirement of cutting the adhesive, when the pressing piece 9 is fully pressed, the length of the blade assembly 1 exceeding the bottom surface of the tool bit seat 8 is not lower than the thickness of the adhesive block.
Fig. 7 shows a structure of a glue carrying piece, which comprises a handle 11 and a glue carrying rod 12, wherein the top surface of the glue carrying rod 12 is provided with a first boss 3, the height of the first boss is the same as that of a second boss 13, the bottom surface of the first boss is connected with a glue carrying base 19 to form a whole, the cross section of the bottom surface of the glue carrying rod is in a convex shape, and the thickness of the glue carrying rod 12 is preferably 1.5mm.
The preferred adhesive cutting device can cut a plurality of adhesive blocks at the same time and cut the adhesive blocks into pieces at one time. During the use, with the intraductal glue piece of a plurality of saving and TE buffer respectively directly pour in each drainage region, TE buffer is followed apopore recycle, and the glue piece moves through buffer platform and puts on the glue piece place the platform, passes through spacing post with the tool bit and agrees with the spacing groove, presses the piece 9 to cut gluey, mentions the tool bit, and a plurality of width accuracy that cut are 2 mm's glue piece bearing and carry gluey pole top surface, pull out and carry gluey piece, take off a plurality of little glue pieces that cut. Simple, quick, accurate and efficient.
When the spring is connected between the bottom of the cutter head seat and the rubber cutting platform in a more preferable mode, the step of taking down and installing the cutter head can be further omitted, the spring compresses the rubber cutting, and the rubber is taken or placed in a free state.
The above are only some specific examples of the present invention, and the technical solution of the present invention is not limited thereto, and each technical feature may be replaced and adjusted equivalently, which falls within the scope of protection of the present invention.
Claims (10)
1. The gel cutting device for the pathogen gel block coated by the pulse field gel electrophoresis is characterized by comprising a gel cutting platform, a cutter head and a gel carrying piece;
The glue cutting platform comprises a cutter head limiting block (5) with a limiting groove (4) and a glue block placing platform (7) with a base groove (6);
The cutter head comprises a pressing piece (9) and a blade assembly (1) fixedly connected with the bottom end of the pressing piece (9) through a fixed iron piece (10), wherein the blade assembly (1) is composed of 2 parallel blades with the interval of 2 mm;
The cutter head also comprises a cutter head seat (8) and a cutter head cover (14), wherein a clamping groove (21) surrounded by iron pieces is formed in the middle of the cutter head seat (8) in the vertical direction, and the clamping groove (21) is mainly used for limiting and supporting the up-and-down movement of the fixed iron pieces (10) and the cutter blade assembly (1); a first spring is arranged between the pressing piece (9) and the cutter head seat (8), the fixed iron piece (10) and the cutter blade assembly (1) can move up and down relative to the cutter head seat (8) in the clamping groove (21) by compressing the first spring, a limit column (2) matched with the limit groove (4) is further arranged on the cutter head seat (8), and the limit column (2) is inserted into the limit groove (4) to enable the cutter head to be in butt joint with the rubber cutting platform; the tool bit seat (8) also comprises a plastic structure fixedly connected with the fixed iron piece (10);
The tool bit cover (14) is provided with a hollowed-out structure for the pressing piece (9) to pass through and a cover top for the main clamping protruding piece (15), and the bottom of the tool bit cover (14) is provided with a bolt hole connected with the tool bit seat (8) through a bolt;
The glue carrying piece comprises a handle (11) and a glue carrying rod (12) which is fixedly connected, the glue carrying rod (12) stretches into the base groove (6) from one side, and the top end of the glue carrying rod is in the same plane with the glue block placing platform (7) and corresponds to the blade assembly (1).
2. The device for cutting the gel block of the pulse field gel electrophoresis coated pathogen according to claim 1, wherein the bottom of the gel carrying rod (12) is connected with the gel carrying base (19) to form an integral structure with a convex cross section, and the cavity of the base groove (6) is matched with the integral structure with the convex cross section.
3. The device for cutting the gel block coated with the pathogen in the pulsed field gel electrophoresis according to claim 1, wherein the thickness of the carrier rod (12) is more than 0mm and less than 2mm, the carrier rod (12) and the gel block placing platform (7) are divided into a plurality of gel cutting areas through a plurality of first bosses (3) and a plurality of second bosses (13) respectively, and the first bosses (3) correspond to the second bosses (13).
4. A device for cutting gel blocks for coating pathogens according to claim 3, wherein the glue carrying rod (12) and the gel block placement platform (7) are divided into 4-6 glue cutting areas.
5. A cutting device for a pulsed field gel electrophoresis coated pathogen gel block according to claim 3, wherein the protruding height of the first boss (3) and the second boss (13) is 1mm, and the thickness of the gel carrying rod (12) is 1.5mm.
6. The gel cutting device for the pulse field gel electrophoresis coated pathogen gel block according to claim 1, wherein a protruding piece (15) is arranged on the outer side of the bottom of the pressing piece (9), the cutter head cover (14) is sleeved outside the pressing piece (9), the cover top is clamped on the protruding piece (15), the cutter head cover (14) is fixedly connected with the cutter head seat (8), and when the pressing piece (9) is not pressed, the first spring is in a free state.
7. The device for cutting the gel block coated with the pathogen by pulsed field gel electrophoresis according to claim 1, wherein the bottom of the tool bit seat (8) is fixedly connected with the gel block placing platform (7) through a second spring.
8. The gel cutting device for the pulsed field gel electrophoresis coated pathogen gel block according to claim 1, wherein the gel cutting platform further comprises a filtering inclined plane (17) connected with the gel block placing platform (7) through a buffer platform (16), a plurality of filtering holes are formed in the filtering inclined plane (17), a water tank is arranged under the inclined plane, and a water outlet hole (18) is externally connected with the water tank.
9. The gel cutting device for the pulsed field gel electrophoresis coated pathogen gel block according to claim 8, wherein the filtering inclined surface (17) and the water tank right below the inclined surface are divided into a plurality of water filtering areas by a plurality of partitions (20), and each area corresponds to one water outlet hole (18).
10. The gel cutting device for pulsed field gel electrophoresis coated pathogen gel blocks according to claim 9, wherein the filtration bevel (17) and the water trough directly under the bevel are divided into 4-6 water filtration areas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810645313.0A CN108858309B (en) | 2018-06-21 | 2018-06-21 | Cutting device of pathogen offset coated by pulsed field gel electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810645313.0A CN108858309B (en) | 2018-06-21 | 2018-06-21 | Cutting device of pathogen offset coated by pulsed field gel electrophoresis |
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| Publication Number | Publication Date |
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| CN108858309A CN108858309A (en) | 2018-11-23 |
| CN108858309B true CN108858309B (en) | 2024-06-21 |
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| CN201810645313.0A Active CN108858309B (en) | 2018-06-21 | 2018-06-21 | Cutting device of pathogen offset coated by pulsed field gel electrophoresis |
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| CN (1) | CN108858309B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116766315B (en) * | 2023-08-18 | 2023-11-10 | 中国医学科学院基础医学研究所 | An automatic rubber block crushing device |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208468453U (en) * | 2018-06-21 | 2019-02-05 | 四川省疾病预防控制中心 | A kind of pulsed field gel electrophoresis coating pathogen blob of viscose cuts adhesive dispenser |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6342143B1 (en) * | 2000-01-06 | 2002-01-29 | Carnegie Mellon University | Cutting tool for multiple sample retrieval from gelatinous material |
| US6606929B2 (en) * | 2001-06-25 | 2003-08-19 | Joseph H. Evreniadis | Apparatus and method for cutting gel and other sheet materials |
| WO2008053916A1 (en) * | 2006-11-01 | 2008-05-08 | Kurume University | Cutting guide device for preparing texture section, texture section preparing device, and method for preparing texture section |
| US8635934B2 (en) * | 2009-09-15 | 2014-01-28 | Jian-Qiang Kong | Microtome |
| CN201724854U (en) * | 2010-08-18 | 2011-01-26 | 中国检验检疫科学研究院 | Mold for pulsed field gel electrophoresis coating pathogen blob of viscose |
| CN204183606U (en) * | 2014-09-03 | 2015-03-04 | 山东鲁润阿胶药业有限公司 | A kind of donkey-hide gelatin dicer |
| CN204604382U (en) * | 2015-05-15 | 2015-09-02 | 四川农业大学 | Adjustable gel cutting cutter |
| CN206154360U (en) * | 2016-07-05 | 2017-05-10 | 广西师范大学 | Ware is surely glued to running gel of adjustable blade interval |
| CN207448558U (en) * | 2017-10-30 | 2018-06-05 | 云南省第一人民医院 | A kind of semi-automatic boxlike cuts adhesive dispenser |
| CN207402841U (en) * | 2017-11-17 | 2018-05-25 | 淮安市疾病预防控制中心 | Pulsed field gel electrophoresis DNA of bacteria embeds the cutter device of blob of viscose |
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2018
- 2018-06-21 CN CN201810645313.0A patent/CN108858309B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208468453U (en) * | 2018-06-21 | 2019-02-05 | 四川省疾病预防控制中心 | A kind of pulsed field gel electrophoresis coating pathogen blob of viscose cuts adhesive dispenser |
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