CN108971220B - Preparation method of pyrite microorganism combined permeable reaction wall - Google Patents
Preparation method of pyrite microorganism combined permeable reaction wall Download PDFInfo
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- 229910052683 pyrite Inorganic materials 0.000 title claims abstract description 35
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 239000011028 pyrite Substances 0.000 title claims abstract description 35
- 244000005700 microbiome Species 0.000 title claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 15
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 15
- 239000003337 fertilizer Substances 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 12
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000004888 barrier function Effects 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
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- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
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- 238000000227 grinding Methods 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 230000032770 biofilm formation Effects 0.000 claims description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 abstract description 7
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 6
- 150000003071 polychlorinated biphenyls Chemical class 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000035699 permeability Effects 0.000 abstract description 3
- 241001388028 Shewanella decolorationis Species 0.000 abstract description 2
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 abstract 2
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 238000006722 reduction reaction Methods 0.000 abstract 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 26
- 239000011651 chromium Substances 0.000 description 14
- 230000001603 reducing effect Effects 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 6
- 229910052742 iron Inorganic materials 0.000 description 6
- 239000012429 reaction media Substances 0.000 description 6
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 5
- 229910052804 chromium Inorganic materials 0.000 description 5
- 238000007873 sieving Methods 0.000 description 5
- 230000009467 reduction Effects 0.000 description 4
- HLQDGCWIOSOMDP-UHFFFAOYSA-N 2,3,4,5-tetrachlorobiphenyl Chemical group ClC1=C(Cl)C(Cl)=CC(C=2C=CC=CC=2)=C1Cl HLQDGCWIOSOMDP-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 239000000460 chlorine Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 239000000945 filler Substances 0.000 description 3
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- 239000002689 soil Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- UQMGJOKDKOLIDP-UHFFFAOYSA-N 3,3',4,4'-tetrachlorobiphenyl Chemical group C1=C(Cl)C(Cl)=CC=C1C1=CC=C(Cl)C(Cl)=C1 UQMGJOKDKOLIDP-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical group C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 238000000643 oven drying Methods 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000005067 remediation Methods 0.000 description 2
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- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010028400 Mutagenic effect Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 231100000693 bioaccumulation Toxicity 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 150000001805 chlorine compounds Chemical class 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
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- 238000009713 electroplating Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 229940095991 ferrous disulfide Drugs 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052595 hematite Inorganic materials 0.000 description 1
- 239000011019 hematite Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
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- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- LIKBJVNGSGBSGK-UHFFFAOYSA-N iron(3+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[Fe+3].[Fe+3] LIKBJVNGSGBSGK-UHFFFAOYSA-N 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
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- 231100000243 mutagenic effect Toxicity 0.000 description 1
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- 239000005416 organic matter Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920001021 polysulfide Polymers 0.000 description 1
- 239000005077 polysulfide Substances 0.000 description 1
- 150000008117 polysulfides Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
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- 238000001179 sorption measurement Methods 0.000 description 1
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/002—Reclamation of contaminated soil involving in-situ ground water treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Fertilizers (AREA)
Abstract
本发明公开了一种黄铁矿微生物联合渗透性反应墙的制备方法。该反应墙由黄铁矿，缓释性营养肥料和生物炭固定化微生物按质量比4:1:4均匀组成。缓释性肥料为市售好康多缓释肥，生物炭由椰子壳在300℃下缺氧灼烧而成。微生物是Shewanella decolorationis。本发明还公布了生物炭固定化微生物的方法。本发明黄铁矿微生物联合渗透性反应墙利用了黄铁矿中Fe(II)还原反应迅速的优点，同时微生物不断繁殖、将生成的Fe(III)还原为Fe(II)保证反应持续进行。该方法操作简单、成本低廉，制得的反应墙对重金属和多氯联苯类有机物均有较高去除率，且使用寿命长。
The invention discloses a preparation method of a pyrite microorganism combined permeability reaction wall. The reaction wall is evenly composed of pyrite, slow-release nutrient fertilizer and biochar immobilized microorganisms in a mass ratio of 4:1:4. The slow-release fertilizer is the commercially available Haokando slow-release fertilizer, and the biochar is made from coconut shells burned in anoxic conditions at 300°C. The microorganism is Shewanella decolorationis. The invention also discloses a method for immobilizing microorganisms with biochar. The pyrite microorganism combined permeability reaction wall of the invention utilizes the advantages of rapid reduction reaction of Fe(II) in pyrite, and at the same time, the microorganisms reproduce continuously, and the generated Fe(III) is reduced to Fe(II) to ensure the continuous progress of the reaction. The method is simple in operation and low in cost, and the prepared reaction wall has a high removal rate for heavy metals and polychlorinated biphenyls, and has a long service life.
Description
Technical Field
The invention relates to a preparation method of a pyrite microorganism combined permeable reaction wall, belonging to the technical field of environmental protection.
Background
The Permeable Reactive Barrier (PRB) technology is a kind of emerging soil and underground water in-situ remediation technology in recent years, has become a main technology for remediating foreign heavy metal pollution at present, and gradually becomes a research focus in the field of remediating soil and underground water in China. The reaction medium is the key of the repair effect of the PRB technology. Mainly comprises redox reaction medium, adsorption reaction medium, precipitation reaction medium and mixed reaction medium.
The zero-valent iron has become one of the most effective reaction media in the in-situ remediation of the water body due to the advantages of high reaction speed, high reduction potential, low price, easy obtainment and the like, is also one of the most common media in the PRB technology, and can effectively reduce and remediate the water body polluted by various organic matters and heavy metals.
A large amount of experiment and field operation data show that the removal capability of zero-valent iron-PRB is reduced along with the lapse of time, and the repair effect is deteriorated. Mainly because the zero-valent iron is oxidized into Fe (III) and Fe (II) in the process of reducing pollutants and hydroxide is generated to cover the surface, and the active sites on the surface of the zero-valent iron are reduced. Meanwhile, the precipitated product is easy to block the pore channels of the wall material, and the permeability of PRB is reduced.
In addition to zero-valent iron, Fe (II) also has reducing properties. Methods for removing organic and heavy metals from water using fe (ii) minerals such as pyrite, hematite, siderite are becoming increasingly important.
Pyrite is a sulfide mineral with the widest distribution in the earth crust, and the main component is ferrous disulfide (FeS)2) Containing Fe having reducibility2+Ions and polysulfides (S)2 2-). Heavy metals and organic matter can be reduced. However, Fe (II) has a reduction potential lower than that of zero-valent iron and is therefore more easily consumed, and if used as a PRB material, has a short service life.
In the beginning of the 20 th century, microbial dissimilarity of Fe (III) reduction has been recognized. The dissimilatory iron reducing microorganisms can oxidize organic matters by taking Fe (III) as an exogenous electron acceptor in the metabolic process, and reduce Fe (III) into Fe (II) in the process. PRB repair is mostly in an anaerobic environment, and if Fe (III) on the surface of the pyrite can be reduced by using dissimilatory iron reducing bacteria to generate Fe (II) with reducibility, the problem of reduction of PRB long-term treatment capacity caused by consumption of Fe (II) in the pyrite can be effectively solved.
However, the specific surface area of the iron ore is not large, so that the growth of microorganisms is not facilitated, and an iron ore-microorganism removing system is difficult to construct.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a preparation method of a pyrite microorganism combined permeable reaction wall. The pollutants in the groundwater and the soil are treated by utilizing the synergistic effect of the pyrite and the dissimilatory iron reducing bacteria. The problem of traditional PRB filler is easily passivated, and the service life is low is solved.
A pyrite-microorganism combined permeable reaction wall is composed of pyrite, slow-release nutrient fertilizer and biochar immobilized microorganism.
Crushing the pyrite by a ball mill to obtain the particle size of 20-40 meshes.
The nutrient fertilizer is a commercial slow-release fertilizer Haokadou.
Burning coconut shell of 2-3cm at 300 deg.C under oxygen deficiency for 2 hr, soaking in 0.1mol/L hydrochloric acid for 12 hr to remove ash, washing with deionized water until pH is constant, and oven drying at 105 deg.C. Grinding to particle size of 20-40 mesh.
The microorganism is Shewanella Decolorationis (MCCC 1A11454), purchased from China center for culture Collection of marine microorganisms, activated and stored on LB slant.
The biochar immobilized microorganism is prepared by the following steps: preparing 10L of sterile culture medium, adding 500g of coconut shell biochar sterilized at 120 ℃ for 20min, inoculating 100mL of S.decoloniones bacterial suspension, standing and culturing for 48h, filtering the coconut shell biochar under the sterile condition, transferring the coconut shell biochar to 10L of fresh sterile culture medium, inoculating 100mL of S.decoloniones bacterial suspension, and standing and culturing for 48 h. And finishing the film formation after ten times of circulation.
The culture medium for preparing the bacterial suspension consists of the following components: 10g/L of glucose; NH (NH)4Cl 1g/L；KH2PO40.5 g/L; 5g/L of yeast powder.
PRB is formed by completely and uniformly mixing pyrite, slow-release nutrient fertilizer, nutrient elements and biochar immobilized microorganisms according to a ratio of 4:1: 4.
Compared with the prior art, the invention has the beneficial effects that:
(1) the method selects pyrite with rich natural content as the reducing filler of PRB, and is cheaper and more easily obtained than zero-valent iron.
(2) The method adopts a pyrite-dissimilatory iron reducing bacteria synergistic system, utilizes the advantage of rapid reaction by a chemical method, simultaneously continuously breeds microorganisms, reduces the generated Fe (III) into Fe (II), ensures that the reaction is continuously carried out, and prolongs the service life of PRB.
(3) The method enriches microorganisms in the biochar through an immobilization technology and then mixes the biochar with the pyrite powder, solves the problems of small specific surface area and difficult film formation of the pyrite, and successfully constructs a pyrite-microorganism reaction system.
(4) The activated carbon is fired by using agricultural waste coconut shells as a raw material, a carbon source is provided for microbial growth, and a nitrogen source and a phosphorus source are provided by using common fertilizers in agriculture. Low cost and environmental protection.
(5) The permeable reactive barrier material disclosed by the invention can be used for treating heavy metals and organic matters, and has a wide application prospect.
Drawings
FIG. 1 is a permeable reaction wall experimental set-up.
FIG. 2 is a graph of the removal rate of the permeable reactive barrier of the present invention within 30 days of treatment at 100mg/LCr (VI).
FIG. 3 shows the removal rate of 3,3 ', 4, 4' -tetrachlorobiphenyl of 10mg/L in 30 days after the treatment of the permeable reaction wall of the present invention.
A permeable reaction wall experimental device 10 and a water inlet area 11; a coarse sand packing region 12; permeable reaction walls 13.
Detailed Description
The permeable reactive barrier experimental device 10 is composed of a water inlet area 11, a coarse sand filling area 12 and a permeable reactive barrier 13. The invention is further described below with reference to the accompanying drawings.
Example 1: preparation of the permeable reactive barrier 13 filler:
purchasing commercial pyrite, crushing and sieving the pyrite by a ball mill, sieving the pyrite by a 20-mesh sieve, sieving the pyrite by a 40-mesh sieve again, and taking the pyrite on the pyrite by a 40-mesh sieve.
Cutting coconut shell to 2-3cm with a cutting machine, placing in a muffle furnace, performing anoxic ignition at 300 deg.C for 2h, soaking to 0.1mol/L hydrochloric acid for 12h, washing with deionized water to remove ash, washing until pH value is constant, and oven drying at 105 deg.C. Grinding, sieving with 20 mesh sieve, sieving with 40 mesh sieve to obtain the oversize product, i.e. charcoal.
LB medium 100mL was prepared, S.deconiorationis stored on a slant was inoculated, and the mixture was cultured for 24 hours.
Weighing 500g of coconut shell biochar, and sterilizing at 121 ℃ for 20 min.
10L of the strain suspension culture medium is prepared. The formula is as follows: 10g/L of glucose; NH (NH)4Cl 1g/L；KH2PO40.5 g/L; 5g/L of yeast powder. Sterilizing at 121 deg.C for 20 min. 500g of sterilized coconut shell charcoal was added and 100mL [0031 ] of the resulting mixture was inoculated]The s.decoloration seed solution obtained in (1). And standing and culturing for 48 hours.
Filtering out coconut shell biochar under aseptic conditions. Transfer to 10L fresh sterile medium, inoculate 100mL fresh S.decoloresoniis bacterial suspension, and culture for 48 h.
Repeating the steps for ten times to obtain the biochar with successful biofilm formation.
The pyrite and the purchased slow release fertilizer are well-mixed, and the biochar immobilized microorganisms are completely and uniformly mixed according to the ratio of 4:1: 4. The permeable reaction wall 13 shown in fig. 1 is installed.
Example 2: the prepared permeable reactive barrier 13 treats wastewater containing Cr (VI).
In recent years, chromium and compounds thereof are widely applied to industries such as metal processing, metallurgy, electroplating, leather making, coating, paint, fertilizer, printing and dyeing and the like, and chromium-containing waste water and waste residues can be generated in the industrial production process. According to incomplete statistics, the chromium slag is discharged by about 60 ten thousand tons every year in China, wherein the discharge or the comprehensive utilization rate after treatment is less than 17 percent. Chromium in water exists mainly in two forms of Cr (VI) and Cr (III). Cr (III) is stable in property, is easy to generate hydroxide precipitate, is an important micronutrient element of a human body, but Cr (VI) has strong mobility and toxicity, can induce cancer, has potential teratogenic and mutagenic effects, and has 100 times higher toxicity to the human body than Cr (III). The water-soluble Cr (VI) is listed as one of 8 chemical substances with the greatest harm to human bodies and is also one of 3 internationally recognized carcinogenic metal substances. Therefore, how to reduce and remove chromium pollution in the environment, especially the very water-soluble cr (vi), becomes a difficult problem and a hotspot in the environmental field.
Preparing raw water containing 100mg/LCr (VI). The concentration of Cr (VI) in the water was measured by spectrophotometry with a peristaltic pump at 10rpm for 30d (FIG. 1) every 24 h. The removal rate was calculated and the measurement results are shown in FIG. 2.
The pyrite microorganism combined permeable reaction wall 13 has a better removal rate to Cr (VI), and the removal rate is always kept above 95% within 30 days of the experiment.
Example 3: the permeable reactive barrier 13 thus produced treats polychlorinated biphenyl.
Polychlorinated biphenyls (PCBs) are artificially synthesized organic compounds, and are chlorides formed by substituting chlorine for hydrogen atoms on a biphenyl ring. Due to its persistence, bioaccumulation, long-range migration and biotoxicity. The first list of controlled pollutants, which may be enriched by the food chain and pose a significant threat to biological and human health, has been listed in the stockholm convention on persistent organic pollutants. The wide industrial use of polychlorinated biphenyls has caused a problem of global environmental pollution.
Preparing raw water containing 10mg/L of 3,3 ', 4, 4' -tetrachlorobiphenyl. Feeding water at a speed of 10rpm for 30d (figure 1) by using a peristaltic pump, sampling every 24h, extracting by using n-hexane, and measuring the concentration of residual tetrachlorobiphenyl in the water by using a high performance liquid chromatography. The removal rate of the PRB to tetrachlorobiphenyl is calculated, and the result is shown in figure 3.
The removal rate of the pyrite microorganism combined permeable reaction wall 13 to tetrachlorobiphenyl is maintained to be about 82% at the initial stage of operation, the effect of microorganisms at the later stage is more and more important, and the removal rate reaches 97% in 30 days.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.
Claims (4)
1. The preparation method of the pyrite microorganism combined permeable reaction wall is characterized by comprising the following components in percentage by mass:
pyrite 4
Slow-release nutrient fertilizer 1
Biochar immobilized microorganism 4;
the biochar is prepared by cutting coconut shells of 2-3cm into 2-3cm, performing anoxic ignition at 300 ℃ for 2h, soaking in 0.1mol/L hydrochloric acid for 12h, washing with deionized water to be neutral, drying at 105 ℃, and grinding to the particle size of 20-40 meshes;
the biochar immobilized microorganism is prepared by the following steps of preparing 10L of sterile culture medium, wherein: glucose 10g/L, NH4Cl 1 g/L，KH2PO40.5g/L, 5g/L yeast powder; adding 500g of coconut shell biochar sterilized at 121 ℃ for 20min, and inoculating 100mLShewanelladecolorationisAnd (3) filtering coconut shell biochar under an aseptic condition after standing and culturing the coconut shell biochar in the bacterial suspension for 48 hours, transferring the coconut shell biochar to 10L of fresh aseptic culture medium, inoculating 100mL of bacterial suspension, standing and culturing for 48 hours, and completing biofilm formation after circulating for ten times.
2. The method for preparing the pyrite microorganism-associated permeable reactive barrier according to claim 1, wherein the microorganism isShewanella decolorationisMCCC 1A11454 is purchased from China marine microorganism culture preservation management center, and is preserved on LB inclined plane after being activated.
3. The method for preparing the pyrite-microorganism combined permeable reactive barrier according to claim 1, wherein the slow-release nutrient fertilizer is a commercially available good kangdao slow-release fertilizer.
4. The method for preparing the pyrite microorganism-associated permeable reaction wall according to claim 1, wherein the pyrite is commercially available pyrite, and the pyrite is ground by a ball mill to have a particle size of 20-40 meshes.
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