CN109363029A - Bamboo leaf-flavored soda water and preparation method thereof - Google Patents
Bamboo leaf-flavored soda water and preparation method thereof Download PDFInfo
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- CN109363029A CN109363029A CN201811293072.4A CN201811293072A CN109363029A CN 109363029 A CN109363029 A CN 109363029A CN 201811293072 A CN201811293072 A CN 201811293072A CN 109363029 A CN109363029 A CN 109363029A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 65
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 title claims abstract description 43
- 235000017166 Bambusa arundinacea Nutrition 0.000 title claims abstract description 36
- 235000017491 Bambusa tulda Nutrition 0.000 title claims abstract description 36
- 241001330002 Bambuseae Species 0.000 title claims abstract description 36
- 235000015334 Phyllostachys viridis Nutrition 0.000 title claims abstract description 36
- 239000011425 bamboo Substances 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 239000000284 extract Substances 0.000 claims abstract description 93
- 235000019640 taste Nutrition 0.000 claims abstract description 34
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 15
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 7
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 7
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 61
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 57
- 235000019441 ethanol Nutrition 0.000 claims description 47
- 238000000605 extraction Methods 0.000 claims description 45
- 239000007787 solid Substances 0.000 claims description 36
- 230000000694 effects Effects 0.000 claims description 34
- 230000004044 response Effects 0.000 claims description 27
- 238000002137 ultrasound extraction Methods 0.000 claims description 23
- 238000004458 analytical method Methods 0.000 claims description 15
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 14
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- 238000005457 optimization Methods 0.000 claims description 9
- 239000013558 reference substance Substances 0.000 claims description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 8
- 238000002604 ultrasonography Methods 0.000 claims description 8
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 claims description 7
- 239000005711 Benzoic acid Substances 0.000 claims description 7
- 235000010233 benzoic acid Nutrition 0.000 claims description 7
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims description 7
- 235000005487 catechin Nutrition 0.000 claims description 7
- 229950001002 cianidanol Drugs 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 7
- 238000009835 boiling Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000011156 evaluation Methods 0.000 claims description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 101000588476 Homo sapiens [heparan sulfate]-glucosamine N-sulfotransferase NDST3 Proteins 0.000 claims description 3
- 102100031395 [heparan sulfate]-glucosamine N-sulfotransferase NDST3 Human genes 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000012982 microporous membrane Substances 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000004704 ultra performance liquid chromatography Methods 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 239000011344 liquid material Substances 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 210000004556 brain Anatomy 0.000 abstract description 3
- 238000003786 synthesis reaction Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 230000003860 sleep quality Effects 0.000 abstract description 2
- LDCYZAJDBXYCGN-VIFPVBQESA-N 5-hydroxy-L-tryptophan Chemical group C1=C(O)C=C2C(C[C@H](N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-VIFPVBQESA-N 0.000 abstract 1
- 229940000681 5-hydroxytryptophan Drugs 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 abstract 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 abstract 1
- 229960003987 melatonin Drugs 0.000 abstract 1
- LDCYZAJDBXYCGN-UHFFFAOYSA-N oxitriptan Natural products C1=C(O)C=C2C(CC(N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052700 potassium Inorganic materials 0.000 abstract 1
- 239000011591 potassium Substances 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 238000013461 design Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 239000013256 coordination polymer Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000005979 Citrus limon Nutrition 0.000 description 3
- 244000131522 Citrus pyriformis Species 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 240000002547 Rosa roxburghii Species 0.000 description 2
- 235000000640 Rosa roxburghii Nutrition 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006385 ozonation reaction Methods 0.000 description 2
- BITYAPCSNKJESK-UHFFFAOYSA-N potassiosodium Chemical compound [Na].[K] BITYAPCSNKJESK-UHFFFAOYSA-N 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000011449 Rosa Nutrition 0.000 description 1
- 241000482268 Zea mays subsp. mays Species 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 210000000476 body water Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/78—Treatment of water, waste water, or sewage by oxidation with ozone
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Environmental & Geological Engineering (AREA)
- Organic Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Botany (AREA)
- Mycology (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
本发明属于食品加工技术领域，公开了一种竹叶口味苏打水及其制备方法。按照质量百分数由碳酸氢钠0.75％、水98.81％、竹叶香精0.14％、非洲加纳籽提取物0.30％组成。本发明制备工艺简单，造价低廉，再制备过程中所用的设备都进行臭氧消毒，确保了水质的纯净与安全，苏打水为弱碱性水，有效维持身体钠钾平衡，在配方中添加了非洲加纳籽提取物，非洲加纳籽提取物中的主要活性成分为5‑羟基色氨酸，能提高脑部5‑HT浓度、抑制忧郁、促进褪黑素合成、改善睡眠质量的功效，添加了竹叶香精，具有竹叶口味。
The invention belongs to the technical field of food processing, and discloses a bamboo leaf-flavored soda water and a preparation method thereof. According to mass percentage, it is composed of sodium bicarbonate 0.75%, water 98.81%, bamboo leaf essence 0.14%, and African Ghana seed extract 0.30%. The preparation process of the invention is simple and the cost is low, and the equipment used in the preparation process is sterilized by ozone, which ensures the purity and safety of the water quality. The soda water is weakly alkaline water, which effectively maintains the balance of sodium and potassium in the body. Ghana seed extract, the main active ingredient in African Ghana seed extract is 5-hydroxytryptophan, which can increase the concentration of 5-HT in the brain, inhibit depression, promote melatonin synthesis, and improve sleep quality. Leaf essence, with the taste of bamboo leaves.
Description
Technical field
The invention belongs to food processing technology fields more particularly to a kind of leaf of bamboo taste soda water and preparation method thereof.
Background technique
Currently, emerging beverage of the soda water as recent years, is held in both hands extensively by public heat, to the status of soda water
And Analysis on Prospect, Learning Studies are carried out to the production technology of soda water in practice in production.There are people more to soda water
Cognition, improve that people's health is horizontal, the development of regional economy is promoted to have important meaning.With market monitor enhancing,
The soda water in China will be fast-developing towards the direction of health.At present only find a kind of Rosa roxburghii Tratt soda water of CN104256797A and
Preparation method is added in carbonated water, the Rosa roxburghii Tratt taste soda water of preparation using Rosa roxburghi Juice；Patent (CN201510970416.0)
A kind of Limon pulp soda water is disclosed, lemon taste is reached by the Limon pulp for adding fresh；Patent
(CN107348278A) a kind of corn soda water is disclosed, the taste of corn is reached by adding sweet corn kernel, is revived at present
Taste of fetching water is single, is mostly lemon soda water, and taste and mouthfeel are not able to satisfy public demand.
Response surface design is a kind of effectively statistics and optimization method, with traditional uniform design compared with orthogonal design,
Response phase method has many advantages, such as that test accuracy is high, test number (TN) is few, mathematical model prediction is good, and can reflect each factor and response
Relationship between value.Therefore the present invention using in chromatogram share peak total peak area as response, with Design-Expert software into
Row Box-Behnken response surface experimental design, optimizes African Ghana's seed extract extraction process, is overall merit Africa
Ghana's seed extract quality provides reliable method.
In conclusion problem of the existing technology is:
Soda water taste is single at present, is mostly lemon soda water, and taste and mouthfeel are not able to satisfy public
Demand.
African Ghana's seed extract extraction effect of the prior art is poor, and the purity of extraction is low.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of leaf of bamboo taste soda water and preparation method thereof.
The invention is realized in this way a kind of leaf of bamboo taste soda water, the leaf of bamboo taste soda water is according to quality percentage
Number is made of sodium bicarbonate 0.75%, water 98.81%, leaf of bamboo essence 0.14%, African Ghana seed extract 0.30%.
Another object of the present invention is to provide a kind of preparation method of leaf of bamboo taste soda water, the leaf of bamboo tastes
Soda water preparation method includes:
Step 1: equipment cleaning: the material-compound tank used in preparation process, pipeline, finished pot are cleaned with Ozone Water, disappeared
Poison, it is spare；
Step 2: ingredient: the sodium bicarbonate of certain mass and African Ghana seed extract are placed in material-compound tank and are added
Dissolved in purified water stirs evenly；
Step 3: by the solution stirred evenly filtering in finished pot, the leaf of bamboo that corrresponding quality is added into finished pot is fragrant
Essence stirs evenly；
Step 4: into step 3 solution be added corrresponding quality pure water, be uniformly mixed to get.
Further, African Ghana's seed extract extracting method includes:
Using the total peak area for sharing peak in liquid chromatogram as index, it is dense that ultrasonic time, ethyl alcohol are investigated by single_factor method
Degree, influence of the liquid-to-solid ratio to African Ghana's seed extract extraction effect, and optimize African Ghana seed extract with response phase method
Ultrasonic extraction process；Response phase method optimize African Ghana's seed extract ultrasonic extraction process optimum condition are as follows: when ultrasonic
Between 20.06min, concentration of alcohol 72.04%, liquid-to-solid ratio 53.38mLg-1。
Further, African Ghana's seed extract extracting method further comprises:
Reference substance solution preparation: precision weighs catechin, African Ghana seed extract, benzoic acid reference substance, adds methanol system
Contain 5.49 μ g of catechin respectively at every 1mL, African 11.79 μ g of Ghana's seed extract, the mixing reference substance of 7.87 μ g of benzoic acid is molten
Liquid shakes up, 4 DEG C of storages, spare.
Further, African Ghana's seed extract extracting method further comprises: the UPLC analysis of African Ghana's seed extract
Condition:
ACQUITYUPLC HSST3 chromatographic column (2.1mm × 100mm, 1.8 μm)；- 0.05% phosphoric acid of mobile phase acetonitrile (A)
Aqueous solution (B), gradient: 0~0.5min, 7%~14%A；0.5~3min, 14%A；3~5.5min, 14%~18%
A；5.5~6min, 18%~20%A；6~9min, 20%~22%A；9~9.5min, 22%~40%A；9.5~11min,
40%~50%A；11~11.5min, 50%~100%A；11.5~12min, 100%A；12~12.5min, 100%~
7%A；12.5~14min, 7%A；Detection wavelength 230nm；30 DEG C of column temperature；Flow velocity 0.4mLmin-1；2 μ L of sample volume.
Further, African Ghana's seed extract extracting method further comprises: African Ghana's seed extract extracting method
Preliminary screening:
0.5g10 parts of African Ghana's seed of 50 meshes were weighed, were placed in boiling flask, it is each that 50% ethyl alcohol 50mL is added, claim
Determine quality, wherein 5 parts of samples distinguish heating and refluxing extraction 0.5,1,1.5,2,2.5h, another 5 parts of samples distinguish ultrasonic extraction 10,
30,50,70,90min；Standing is extracted to room temperature, weighed to supply quality, 0.22 μm of filtering with microporous membrane takes subsequent filtrate, sample introduction
Detection；The unit crude drug for selecting 11 shared peaks of S/N > 20 in chromatogram as evaluation index, and being handled with ultrasonic 30min
Peak area is 100%, calculates the shared peak peak area recovery rate of different disposal sample.
Further, African Ghana's seed extract extracting method further comprises: African Ghana's seed extract ultrasonic extraction
It specifically includes:
Negated continent Ghana seed crushes, and crosses 50 meshes, weighs, set in boiling flask, the appropriate amount of ethanol of respective concentration is added, and claims
Fixed, ultrasonic extraction, 40kHz, 150W take out and stand to room temperature, and weighed quality of supplying is to get African Ghana's seed extract.
Further, the ultrasonic extraction time includes: to the impact analysis method of extraction effect
Fixed liquid-to-solid ratio is 100: 1 (mLg- 1), concentration of alcohol 50%, selection ultrasound 10,20,30,40,50min into
Row test；With the extension of ultrasonic time, shared peak total peak area is gradually increased；After ultrasonic 20min, shared peak total peak area becomes
In steady, extraction tends to be complete.
Further, concentration of alcohol includes: to the impact analysis method of extraction effect
Fixed liquid-to-solid ratio is 100: 1 (mLg-1), ultrasonic time 20min, selects 20%, 35%, 50%, 65%,
80% concentration of alcohol extracts test to African Ghana's seed extract；When concentration of alcohol is 65%, the face Zong Feng at peak is shared
Product reaches peak value；When concentration of alcohol is 80%, extraction effect is weakened, therefore is selected 65% for optimal concentration of alcohol；
Liquid-to-solid ratio includes: to the impact analysis method of extraction effect
Fixed ultrasonic time is 20min, concentration of alcohol 50%, selects liquid-to-solid ratio 50: 1,100: 1,250: 1,500: 1,
800∶1(mL·g- 1) tested；With the increase of liquid-to-solid ratio, the total peak area at shared peak is gradually increased；When liquid-to-solid ratio is greater than
100∶1(mL·g- 1), the growth trend of shared peak total peak area slows down, and extraction tends to be complete；Select 100: 1 (mLg- 1) it is more excellent
Liquid-to-solid ratio.
Further, during African Ghana's seed extract extracts, using Design-Expert8.0.6 software to experimental data into
Row variance analysis and quadratic polynomial regression fit, regression model P are 0.0032, and model is extremely significant；Losing quasi- item P is 0.2360,
It is not significant；The coefficient of determination is 0.9677, and models fitting degree is good；Factor A, B, B2, C2 is to significantly affect factor, is added to Africa
Seed extract extraction effect of receiving is affected；Model is optimized manually, on the basis of original fit equation, is removed not significant
After item BC and A2, optimization gained actual value equation:
R=2.2806 × 107+7.8125×104A+4.7447×108B+1.5284×108C-1.3857 × 103AB-
1.6157×103AC-3.1633 × 104B2-3.0159 × 104C2。
In conclusion advantages of the present invention and good effect are as follows:
Leaf of bamboo taste soda water provided by the invention and preparation method thereof, soda water preparation process is simple, cheap,
Equipment used in preparation process all carries out ozonization again, it is ensured that water quality it is pure with it is safe, soda water is alkalescent water,
Body sodium-potassium balance is effectively maintained, is added to African Ghana's seed extract in formula, it is main in African Ghana's seed extract
Active constituent is 5HTP, can improve brain 5-HT concentration, inhibit melancholy, promote epiphysin synthesis, improve sleep matter
The effect of amount, is added to leaf of bamboo essence, increases the taste of soda water, no matter from taste or mouthfeel all than on the market other
Soda water is even better.
The present invention selects ultrasonic method to study African Ghana's seed extract extraction process.To be shared in liquid chromatogram
The total peak area at peak is index, investigates ultrasonic time, concentration of alcohol, liquid-to-solid ratio to African Ghana's seed extract by single_factor method
The influence of extraction effect, and with the ultrasonic extraction process of the African Ghana's seed extract of response phase method optimization.The experimental results showed that
Influence of the concentration of alcohol to African Ghana's seed extract extraction effect is maximum, followed by liquid-to-solid ratio and ultrasonic time.Response phase method
Optimize African Ghana's seed extract ultrasonic extraction process optimum condition are as follows: ultrasonic time 20.06min, concentration of alcohol
72.04%, liquid-to-solid ratio 53.38mLg-1；The model predication value that peak total peak area is shared in chromatogram is 2.1608 × 108.It is logical
Cross confirmatory experiment discovery: under this optimum extraction condition, in chromatogram share peak total peak area actual value be 2.1422 ×
108, close with predicted value, the two relative deviation is 0.86%, illustrates that the resulting African Ghana seed extract of response surface optimization mentions
Taking technique is stable, feasible.From African Ghana's seed extract extraction effect completely, sufficiently from the point of view of, the present invention gained technique compared with
" Chinese Pharmacopoeia " contained " African Ghana's seed extract " extracting method has more advantage, can be used for opening for African Ghana's seed extract
Hair, utilization and quality overall evaluation
Detailed description of the invention
Fig. 1 is the flow diagram of the preparation method of leaf of bamboo taste soda water provided in an embodiment of the present invention；
Fig. 2 is influence diagram of the ultrasonic time provided in an embodiment of the present invention to African Ghana's seed extract extraction effect.
Fig. 3 is influence diagram of the concentration of alcohol provided in an embodiment of the present invention to African Ghana's seed extract extraction effect.
Fig. 4 is influence diagram of the liquid-to-solid ratio provided in an embodiment of the present invention to African Ghana's seed extract extraction effect.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
Structure of the invention is explained in detail with reference to the accompanying drawing.
Leaf of bamboo taste soda water provided in an embodiment of the present invention is according to mass percent by sodium bicarbonate 0.75%, water
98.81%, leaf of bamboo essence 0.14%, African Ghana seed extract 0.30% form；Wherein, sodium bicarbonate 0.34g/L.
As shown in Figure 1, the preparation method of leaf of bamboo taste soda water provided in an embodiment of the present invention the following steps are included:
S101: equipment cleaning: the material-compound tank used in preparation process, pipeline, finished pot being cleaned with Ozone Water, sterilized,
It is spare；
S102: ingredient: the sodium bicarbonate of certain mass and African Ghana's seed extract are placed in material-compound tank be added it is pure
Water purification dissolution, stirs evenly；
S103: by the solution stirred evenly filtering in finished pot, the leaf of bamboo essence of corrresponding quality being added into finished pot,
It stirs evenly；
S104: into S103 solution be added corrresponding quality pure water, be uniformly mixed to get.
Below with reference to effect, the invention will be further described.
Preparation process of the present invention is simple, cheap, then equipment used in preparation process all carries out ozonization, it is ensured that
Water quality it is pure with it is safe, soda water is alkalescent water, effectively maintains body sodium-potassium balance, be in formula added to Africa plus
Receive seed extract, the main active in African Ghana's seed extract is 5HTP, can improve brain 5-HT concentration,
Inhibit melancholy, promote epiphysin synthesis, improve the effect of sleep quality, is added to leaf of bamboo essence, there is leaf of bamboo taste.
Africa Ghana seed extract extracting method provided in an embodiment of the present invention includes:
Using the total peak area for sharing peak in liquid chromatogram as index, it is dense that ultrasonic time, ethyl alcohol are investigated by single_factor method
Degree, influence of the liquid-to-solid ratio to African Ghana's seed extract extraction effect, and optimize African Ghana seed extract with response phase method
Ultrasonic extraction process；Response phase method optimize African Ghana's seed extract ultrasonic extraction process optimum condition are as follows: when ultrasonic
Between 20.06min, concentration of alcohol 72.04%, liquid-to-solid ratio 53.38mLg-1。
Below with reference to concrete analysis, the invention will be further described.
1, acetonitrile, methanol are chromatographically pure (German Merck company)；Phosphoric acid be analyze it is pure (Tianjin recover fine chemistry industry grind
Study carefully institute)；95% ethyl alcohol is medicinal alcohol (Yuan Hong medicinal alcohol Co., Ltd of Beijing).
Catechin (purity 97.2%, lot number 110877-201203), benzoic acid (purity 100%, lot number 100419-
201312)；
ACQUITYUPLCTM liquid chromatograph, including binary solvent management system, sample introduction management system, column management system,
Generation of tunable UV detector and Empower3.0 chromatographic work station (Wa-ters company of the U.S.)；KQ3200E type ultrasonic cleaner
(Kunshan Ultrasonic Instruments Co., Ltd.)；XS204 type analysis balance (Met-tlerToledo company of the U.S.)；JJ1000 type electronics
Balance (Shuan Jie test equipment factory of Changshu City).
2 methods and result
The preparation of 2.1 reference substance solutions:
It is appropriate that precision weighs catechin, African Ghana's seed extract, benzoic acid reference substance, adds methanol that every 1mL difference is made
Containing 5.49 μ g of catechin, African 11.79 μ g of Ghana's seed extract, the mixed reference substance solution of 7.87 μ g of benzoic acid is shaken up, 4 DEG C
Storage, it is spare.
The UPLC analysis condition of 2.2 African Ghana seed extracts
ACQUITYUPLC HSST3 chromatographic column (2.1mm × 100mm, 1.8 μm)；- 0.05% phosphoric acid of mobile phase acetonitrile (A)
Aqueous solution (B), gradient: 0~0.5min, 7%~14%A；0.5~3min, 14%A；3~5.5min, 14%~18%
A；5.5~6min, 18%~20%A；6~9min, 20%~22%A；9~9.5min, 22%~40%A；9.5~11min,
40%~50%A；11~11.5min, 50%~100%A；11.5~12min, 100%A；12~12.5min, 100%~
7%A；12.5~14min, 7%A.Detection wavelength 230nm；30 DEG C of column temperature；Flow velocity 0.4mLmin-1；2 μ L of sample volume.
The preliminary screening of 2.3 African Ghana seed extract extracting methods
Precision weighs 0.5g10 parts of African Ghana's seed (crossing 50 meshes), is placed in boiling flask, each that 50% ethyl alcohol is added
50mL, weighed quality, wherein 5 parts of samples distinguish heating and refluxing extraction 0.5,1,1.5,2,2.5h, ultrasound mentions another 5 parts of samples respectively
Take 10,30,50,70,90min.Standing is extracted to room temperature, weighed to supply quality, 0.22 μm of filtering with microporous membrane takes continuous filter
Liquid, sample detection.The list for selecting 11 shared peaks of S/N > 20 in chromatogram as evaluation index, and being handled with ultrasonic 30min
Position crude drug peak area be 100%, calculate different disposal sample shared peak peak area recovery rate, be compared, by with compare
Product map corresponds to retention time can initial guess；Comprehensively consider the factors such as chemical composition stability, operation convenience, selection ultrasound
Method is the extracting method of African Ghana seed extract.
The process flow of 2.4 African Ghana seed extract ultrasonic extractions
Negated continent Ghana seed crushes, and crosses 50 meshes, and precision is weighed in right amount, set in boiling flask, and the second of respective concentration is added
Appropriate alcohol, weighed, ultrasonic extraction (40kHz, 150W) are taken out and are stood to room temperature, it is weighed supply quality to get.
The single factor experiment of 2.5 African Ghana seed extract ultrasonic extraction process parameters
Using 11 of S/N > 20 in chromatogram shared peak total peak areas as evaluation index, different ultrasounds is investigated respectively and is mentioned
Take time (10,20,30,40,50min), concentration of alcohol (20%, 35%, 50%, 65%, 80%) and liquid-to-solid ratio (50: 1,100
: 1,250: 1,500: 1,800: 1mLg-1) influence to African Ghana's seed extract extraction efficiency.
2.5.1 influence fixer of the ultrasonic extraction time to extraction effect
Material is than being 100: 1 (mLg- 1), concentration of alcohol 50%, selection ultrasound 10,20,30,40,50min is tried
It tests, as a result sees Fig. 2.With the extension of ultrasonic time, shared peak total peak area is gradually increased；But after ultrasound 20min, it is total to share peak
Peak area tends to be steady, and extraction tends to be complete.To prevent ultrasonic time too long, Extracting temperature is caused to increase, African Ghana's seed mentions
It takes effective component in object to degrade, therefore selects 20min for optimal ultrasonic time.
2.5.2 the fixed liquid-to-solid ratio of influence of the concentration of alcohol to extraction effect is 100: 1 (mLg-1), and ultrasonic time is
20min selects 20%, 35%, 50%, 65%, and 80% concentration of alcohol extracts test to African Ghana's seed extract, as a result
See Fig. 3.When concentration of alcohol is 65%, the total peak area at shared peak reaches peak value；When concentration of alcohol is 80%, extraction effect
Weakened again, therefore is selected 65% for optimal concentration of alcohol.
2.5.3 influence of the liquid-to-solid ratio to extraction effect
Fixed ultrasonic time is 20min, concentration of alcohol 50%, selects liquid-to-solid ratio 50: 1,100: 1,250: 1,500: 1,
800∶1(mL·g- 1) tested, as a result see Fig. 4.With the increase of liquid-to-solid ratio, the total peak area at shared peak is gradually increased, because
Constantly increase for Extraction solvent volume, the contact surface of medicinal powder and solvent molecule also constantly increases, and extracted amount is caused constantly to increase
It is more.When liquid-to-solid ratio is greater than 100: 1 (mLg- 1), the growth trend of shared peak total peak area slows down, and extraction tends to be complete；But it considers
Being significantly increased for liquid-to-solid ratio may generate certain influence to extracting solution pH, make sensitive to pH value in African Ghana's seed extract
The content of material such as aromatic acid change, therefore select 100: 1 (mLg- 1) it is more excellent liquid-to-solid ratio.
2.6 response phase methods optimize African Ghana's seed extract ultrasonic extraction process test
On the basis of single factor experiment, the level value of 3 liquid-to-solid ratio, concentration of alcohol and ultrasonic time factors is determined, use
Design-Expert8.0.6 software carries out the design of Box-Behnken response surface and data analysis.Specific experiment factor and each water
Level values are shown in Table 1.
1 response surface experiments factor of table and level
According to the Combination Design principle of Box-Behnken, using 3 factor of De-sign-Expert8.0.6 software design, 3 water
Totally 15 experiments are put down, wherein central point 3 repeated experiments；The total peak area at peak is shared as response using S/N > 20 in chromatogram
(R), specific experiment design scheme and 2 be the results are shown in Table.
2 response surface experimental result of table
Variance analysis and quadratic polynomial regression fit are carried out to experimental data using Design-Expert8.0.6 software,
Regression model P is 0.0032, illustrates that model is extremely significant；Losing quasi- item P is 0.2360, not significantly；The coefficient of determination is 0.9677, explanation
The models fitting degree is good；Factor A, B, B2, C2 is to significantly affect factor, is illustrated to African Ghana's seed extract extraction effect
It is affected.For reduced equation, model is optimized manually, on the basis of original fit equation, remove not significant item BC with
After A2, the results of analysis of variance is shown in Table 3, optimizes resulting actual value equation:
R=2.2806 × 107+7.8125×104A+4.7447×108B+1.5284×108C-1.3857 × 103AB-
1.6157×103AC-3.1633 × 104B2-3.0159 × 104C2。
Analysis of variance table after table 3 optimizes
。
Response surface curved surface can more intuitively reflect each factor and influence of the reciprocation of factor to response two-by-two.Africa
In the contour map and response surface 3D map of Ghana's seed extract ultrasonic extraction.Concentration of alcohol extracts African Ghana's seed extract
Influential effect is maximum, followed by liquid-to-solid ratio, and influence of the ultrasonic time to African Ghana's seed extract extraction effect is minimum.Liquid-to-solid ratio
It is affected with the reciprocation of concentration of alcohol to African Ghana's seed extract extraction effect, is secondly liquid-to-solid ratio and ultrasonic time
Reciprocation, the reciprocation of concentration of alcohol and ultrasonic time is least significant.
With the resulting African Ghana seed extract ultrasonic extraction optimised process of Design-Expert 8.0.6 software optimization
Are as follows: liquid-to-solid ratio 53.38mLg- 1, concentration of alcohol 72.04%, ultrasonic time 20.06min, with this condition share the face peak Zong Feng
Long-pending predicted value is 2.160 8 × 108。
2.7 verification test
For the reliability of auth response face method acquired results, the process conditions of model optimization are verified.In view of reality
The feasibility and convenience tested select technological parameter: solid-liquid ratio 53mLg- 1, concentration of alcohol 72%, ultrasonic time 20min into
Row experiment 3 times, the results are shown in Table 4, illustrate equation and the fitting of actual experimental situation preferably, the process of optimization, feasible.Compare
Each the mentioning by contained content assaying method and Optimized Extraction Process of African Ghana's seed extract in " Chinese Pharmacopoeia " (version in 2010)
Take effect.Test solution 3 is prepared by CP method (liquid-to-solid ratio 500mLg-1, concentration of alcohol 50%, ultrasonic time 30min)
Part, sample detection records chromatogram, is compared with confirmatory experiment result.As a result, it has been found that: it is resulting that response phase method optimizes technique
Shared peak total peak area is high compared with CP method by 10.84%；The resulting African Ghana's seed extract peak area of response surface optimization technique compared with
CP method low 1.30%, no significant difference, and liquid material is bigger in CP method lead in extracting solution each ingredient response relatively
Low, peak number is less in chromatogram.Therefore, from the point of view of African Ghana's seed extract quality thoroughly evaluating, response phase method optimization
Resulting Africa Ghana seed extract ultrasonic extraction process has more advantage compared with pharmacopeia extraction method.
Table 4
。
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (10)
1. a kind of leaf of bamboo taste soda water, which is characterized in that the leaf of bamboo taste soda water is according to mass percent by bicarbonate
Sodium 0.75%, water 98.81%, leaf of bamboo essence 0.14%, African Ghana seed extract 0.30% form.
2. a kind of preparation method of leaf of bamboo taste soda water as described in claim 1, which is characterized in that the leaf of bamboo taste soda
Water preparation method includes:
Step 1: equipment cleaning: the material-compound tank used in preparation process, pipeline, finished pot being cleaned with Ozone Water, sterilized, standby
With；
Step 2: ingredient: the sodium bicarbonate of certain mass and African Ghana's seed extract are placed in material-compound tank be added it is pure
Water dissolution, stirs evenly；
Step 3: by the solution stirred evenly filtering in finished pot, the leaf of bamboo essence of corrresponding quality is added into finished pot, stirs
It mixes uniformly；
Step 4: into step 3 solution be added corrresponding quality pure water, be uniformly mixed to get.
3. the preparation method of leaf of bamboo taste soda water as claimed in claim 2, which is characterized in that African Ghana's seed extract mentions
The method is taken to include:
Using the total peak area for sharing peak in liquid chromatogram as index, ultrasonic time, concentration of alcohol, liquid are investigated by single_factor method
Material compares the influence of African Ghana seed extract extraction effect, and optimizes the ultrasound of African Ghana seed extract with response phase method
Extraction process；Response phase method optimize African Ghana's seed extract ultrasonic extraction process optimum condition are as follows: ultrasonic time
20.06min, concentration of alcohol 72.04%, liquid-to-solid ratio 53.38mLg-1。
4. the preparation method of leaf of bamboo taste soda water as claimed in claim 3, which is characterized in that African Ghana's seed extract mentions
The method is taken to further comprise:
Reference substance solution preparation: precision weighs catechin, African Ghana seed extract, benzoic acid reference substance, and methanol is added to be made often
1mL contains 5.49 μ g of catechin, African 11.79 μ g of Ghana's seed extract respectively, and the mixed reference substance solution of 7.87 μ g of benzoic acid is shaken
Even, 4 DEG C of storages are spare.
5. the preparation method of leaf of bamboo taste soda water as claimed in claim 3, which is characterized in that African Ghana's seed extract mentions
The method is taken to further comprise: the UPLC analysis condition of African Ghana's seed extract:
ACQUITYUPLC HSST3 chromatographic column (2.1mm × 100mm, 1.8 μm)；Mobile phase acetonitrile (A) -0.05% phosphoric acid is water-soluble
Liquid (B), gradient: 0~0.5min, 7%~14%A；0.5~3min, 14%A；3~5.5min, 14%~18%A；5.5
~6min, 18%~20%A；6~9min, 20%~22%A；9~9.5min, 22%~40%A；9.5~11min, 40%
~50%A；11~11.5min, 50%~100%A；11.5~12min, 100%A；12~12.5min, 100%~7%A；
12.5~14min, 7%A；Detection wavelength 230nm；30 DEG C of column temperature；Flow velocity 0.4mLmin-1；2 μ L of sample volume.
6. the preparation method of leaf of bamboo taste soda water as claimed in claim 3, which is characterized in that African Ghana's seed extract mentions
The method is taken to further comprise: the preliminary screening of African Ghana's seed extract extracting method:
0.5g10 parts of African Ghana's seed of 50 meshes were weighed, were placed in boiling flask, it is each that 50% ethyl alcohol 50mL, weighed matter is added
Amount, wherein 5 parts of samples difference heating and refluxing extractions 0.5,1,1.5,2,2.5h, another 5 parts of samples difference ultrasonic extraction 10,30,50,
70,90min；Standing is extracted to room temperature, weighed to supply quality, 0.22 μm of filtering with microporous membrane takes subsequent filtrate, sample detection；
The unit crude drug peak area for selecting 11 shared peaks of S/N > 20 in chromatogram as evaluation index, and being handled with ultrasonic 30min
It is 100%, calculates the shared peak peak area recovery rate of different disposal sample.
7. the preparation method of leaf of bamboo taste soda water as claimed in claim 3, which is characterized in that African Ghana's seed extract mentions
Take the method to further comprise: African Ghana's seed extract ultrasonic extraction specifically includes:
Negated continent Ghana seed crushes, and crosses 50 meshes, weighs, set in boiling flask, the appropriate amount of ethanol of respective concentration is added, weighed,
Ultrasonic extraction, 40kHz, 150W take out and stand to room temperature, and weighed quality of supplying is to get African Ghana's seed extract.
8. the preparation method of leaf of bamboo taste soda water as claimed in claim 7, which is characterized in that the ultrasonic extraction time is to extraction
The impact analysis method of effect includes:
Fixed liquid-to-solid ratio is 100: 1 (mLg- 1), concentration of alcohol 50%, selection ultrasound 10,20,30,40,50min is tried
It tests；With the extension of ultrasonic time, shared peak total peak area is gradually increased；After ultrasonic 20min, shared peak total peak area tends to be flat
Surely, it extracts and tends to be complete.
9. the preparation method of leaf of bamboo taste soda water as claimed in claim 7, which is characterized in that concentration of alcohol is to extraction effect
Impact analysis method include:
Fixed liquid-to-solid ratio is 100: 1 (mLg-1), ultrasonic time 20min, selects 20%, 35%, 50%, 65%, 80%
Concentration of alcohol extracts test to African Ghana's seed extract；When concentration of alcohol is 65%, the total peak area at shared peak reaches
To peak value；When concentration of alcohol is 80%, extraction effect is weakened, therefore is selected 65% for optimal concentration of alcohol；
Liquid-to-solid ratio includes: to the impact analysis method of extraction effect
Fixed ultrasonic time is 20min, and concentration of alcohol 50% selects liquid-to-solid ratio 50: 1,100: 1,250: 1,500: 1,800: 1
(mL·g- 1) tested；With the increase of liquid-to-solid ratio, the total peak area at shared peak is gradually increased；When liquid-to-solid ratio is greater than 100: 1
(mL·g- 1), the growth trend of shared peak total peak area slows down, and extraction tends to be complete；Select 100: 1 (mLg- 1) it is more excellent liquid material
Than.
10. the preparation method of leaf of bamboo taste soda water as claimed in claim 7, which is characterized in that African Ghana's seed extract
In extraction, variance analysis and quadratic polynomial regression fit are carried out to experimental data using Design-Expert8.0.6 software,
Regression model P is 0.0032, and model is extremely significant；Losing quasi- item P is 0.2360, not significantly；The coefficient of determination is 0.9677, models fitting
Degree is good；Factor A, B, B2, C2 is to significantly affect factor, is affected to African Ghana's seed extract extraction effect；To mould
Type is optimized manually, on the basis of original fit equation, after removing not significant item BC and A2, and optimization gained actual value equation:
R=2.2806 × 107+7.8125×104A+4.7447×108B+1.5284×108C-1.3857 × 103AB-
1.6157×103AC-3.1633 × 104B2-3.0159 × 104C2。
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