Summary of the invention
The present invention is in order to solve the defective of extremely toxic substance serious harm health, contaminated environment in the existing coating agent for seed, and a kind of nuisance free coating agent for seeds and preparation method thereof is provided.Nuisance free coating agent for seeds is that volumetric concentration is that 0.1~3% shitosan and/or oligopolymerization chitosan sugar juice are made by 0.001~31% additive and surplus by mass percentage; Wherein additive is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.Nuisance free coating agent for seeds is prepared by following step: (one) puts into volumetric concentration by 1: 32.3~999 volume ratio with shitosan and/or chitosan oligomer is 0.1~5% acid solution, makes shitosan and/or oligopolymerization chitosan sugar juice; (2) add the additive that accounts for nuisance free coating agent for seeds solution gross mass 0.001~31%, stir, promptly obtain nuisance free coating agent for seeds; Wherein the additive in the step (two) is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.
Composition in the additive of the present invention extracts in animal, plant or microbial body, and no extremely toxic substance and heavy metal in the raw material are natural, renewable resources, and raw material sources extensively, be easy to get.Technology of the present invention is simple, and is workable, and the comprehensive utilization cost is low; The coating agent for seed of preparing has no adverse effects to people and animals, environment, is a kind of nuisanceless, Green Product.The coating agent for seed that the present invention prepares is easy to use, can directly spray or soak pending seed, just can use after drying; Nuisance free coating agent for seeds of the present invention also can be used for spraying or soaking root, stem and the leaf of vegetables except that spraying or soak the seed, and effect is desirable equally; But also can earlier seed be put into concentration is that the herb liquid that 0.01~1g/mL has a disease and insect resistance effect soaks 10min~4h, and seed dries the back and sprays or soak nuisance free coating agent for seeds of the present invention.Use seed of the present invention and common blank sample seed compares experiment under equivalent environment, using plant of the present invention damage by disease and insect in process of growth reduces more than 60%, growth rate speeds 5~10%, branches and leaves are luxuriant, completely filled fruit, beautiful in colour, mouthfeel good, quality is apparently higher than control group, output also improves 10~15%.
Embodiment
Embodiment one: the present embodiment nuisance free coating agent for seeds is that volumetric concentration is that 0.1~3% shitosan and/or oligopolymerization chitosan sugar juice are made by 0.001~31% additive and surplus by mass percentage; Wherein additive is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.
Shitosan in the present embodiment nuisance free coating agent for seeds and chitosan oligomer can with the heavy metal element chelating in soil or the water, alleviate the absorption of plant to heavy metal element, can avoid a large amount of depositions of heavy metal in human body indirectly.
Embodiment two: the difference of present embodiment and embodiment one is: additive is that the catalase of 500~3000U/mg is formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8% and 0.0025~3%.Other is identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is: additive is the adenylate that accounts for nuisance free coating agent for seeds gross mass 0.002~5%.Other is identical with embodiment one.
The adenylate that present embodiment adds can be regulated plant growth rate, promotes volume increase and the precocity of crops.
Embodiment four: the difference of present embodiment and embodiment one is: additive is that the enzyme work that accounts for nuisance free coating agent for seeds gross mass 0.0025~3% is the catalase of 500~3000U/mg.Other is identical with embodiment one.
The adding catalase helps removing harmful substance and the additional soil nutrient in the growing process in the present embodiment.
Embodiment five: the difference of present embodiment and embodiment one is: additive is the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%.Other is identical with embodiment one.
The insect antimicrobial peptide that adds in the present embodiment helps promoting plant growing and prevention and elimination of disease and pests (comprising bacterium, virus and harmful fungoid), and long-time use also is difficult for developing immunity to drugs, and is that small molecular protein is easy to decompose, absorb.
Embodiment six: the difference of present embodiment and embodiment one is: additive is made up of the adenylate of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8% and 0.002~5%.Other is identical with embodiment one.
Embodiment seven: the difference of present embodiment and embodiment one is: additive is that the catalase of 500~3000U/mg and 0.002~5% adenylate are formed by the enzyme work that accounts for nuisance free coating agent for seeds gross mass 0.0025~3%.Other is identical with embodiment one.
Embodiment eight: the difference of present embodiment and embodiment one is: additive is the rhizobium that account for nuisance free coating agent for seeds gross mass 0.1~15%.Other is identical with embodiment one.
Add rhizobium in the present embodiment and help strengthening the nodulation and nitrogen fixation performance, reduce the nitrogenous fertilizer usage amount, the fertile soil of supporting strengthens disease-resistant crops, resistance, improves crops quality, increases output more than 10%.
Embodiment nine: the difference of present embodiment and embodiment one is: additive is made up of the rhizobium that account for nuisance free coating agent for seeds gross mass 0.1~15%, 0.001~8% insect antimicrobial peptide and 0.002~5% adenylate.Other is identical with embodiment one.
Embodiment ten: the difference of present embodiment and embodiment one is: additive is that the catalase of 500~3000U/mg is formed by the enzyme work of the rhizobium that account for nuisance free coating agent for seeds gross mass 0.1~15% and 0.0025~3%.Other is identical with embodiment one.
Embodiment 11: the difference of present embodiment and embodiment one is: rhizobium are rhizobium of bean, peanut rhizobium, rhizobium melioti or herbage rhizobium; Effective bacterium number in every gram rhizobium 〉=200,000,000.Other is identical with embodiment one.
Soak the beans crop seed with the nuisance free coating agent for seeds that adds rhizobium of bean in the present embodiment, soak peanut seed with the nuisance free coating agent for seeds that adds peanut rhizobium in the present embodiment, soak alfalfa seed with the nuisance free coating agent for seeds that adds rhizobium melioti in the present embodiment, soak grass seed, crop yield 20~60% with the nuisance free coating agent for seeds that adds the herbage rhizobium in the present embodiment.
Embodiment 12: the difference of present embodiment and embodiment one is: additive is that catalase, 0.001~8% insect antimicrobial peptide and 0.002~5% the adenylate of 500~3000U/mg formed by the enzyme work that accounts for nuisance free coating agent for seeds gross mass 0.0025~3%.Other is identical with embodiment one.
Embodiment 13: the difference of present embodiment and embodiment one is: nuisance free coating agent for seeds is that volumetric concentration is that 1~2.5% chitosan solution is made by 0.1~25% additive and surplus by mass percentage.Other is identical with embodiment one.
Select for use chitosan solution to be of value to the release of antimicrobial component in the seed coating medicine in the present embodiment, have antibiotic preferably, antivirus action.Use its antibiotic, that the anti-virus ability ratio uses common coating agent for seed plant raising 25~35% of plant of present embodiment.
Embodiment 14: the difference of present embodiment and embodiment one is: nuisance free coating agent for seeds is that volumetric concentration is that 1~2.5% oligopolymerization chitosan sugar juice is made by 1~25% additive and surplus by mass percentage.Other is identical with embodiment one.
Select for use the oligopolymerization chitosan sugar juice to help the slow release of each composition in the seed coating medicine in the present embodiment, long duration of action, its additive effective time is than common coating agent for seed long 20~30%.
Embodiment 15: the difference of present embodiment and embodiment one is: nuisance free coating agent for seeds is that volumetric concentration is that 1~2.5% shitosan and chitosan oligomer mixed solution are made by 4~20% additive and surplus by mass percentage.Other is identical with embodiment one.
Select for use shitosan and chitosan oligomer mixed solution can improve antibiotic, the antivirus action of seed coating medicine in the present embodiment, can slowly discharge the composition in the seed coating medicine again.
Embodiment 16: the difference of present embodiment and embodiment one is: the deacetylation of shitosan is 70~90%.Other is identical with embodiment one.
Embodiment 17: present embodiment is carried out the preparation of nuisance free coating agent for seeds by following step: (one) puts into volumetric concentration by 1: 32.3~999 volume ratio with shitosan and/or chitosan oligomer is 0.1~5% acid solution, makes shitosan and/or oligopolymerization chitosan sugar juice; (2) add the additive that accounts for nuisance free coating agent for seeds solution gross mass 0.001~31%, stir, promptly obtain nuisance free coating agent for seeds; Wherein the additive in the step (two) is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.
The nuisance free coating agent for seeds that present embodiment makes soaked the field crop seed 10 minutes~7 days, can sow after the field crop seed dries.The nuisance free coating agent for seeds that present embodiment makes soaked the vegetables seed 10 minutes~7 days, can sow after the vegetables seed dries.
Embodiment 18: the difference of present embodiment and embodiment 17 is: the acid solution in the step () is organic acid soln or inorganic acid solution.Other is identical with embodiment 17.
Embodiment 19: the difference of present embodiment and embodiment 17 is: the acid solution in the step () is a glacial acetic acid solution.Other is identical with embodiment 17.