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CN101049109A - Nuisance free coating agent for seeds and preparation method - Google Patents

Nuisance free coating agent for seeds and preparation method Download PDF

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Publication number
CN101049109A
CN101049109A CN200610009904.6A CN200610009904A CN101049109A CN 101049109 A CN101049109 A CN 101049109A CN 200610009904 A CN200610009904 A CN 200610009904A CN 101049109 A CN101049109 A CN 101049109A
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coating agent
seed coating
pollution
free seed
chitosan
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CN200610009904.6A
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Chinese (zh)
Inventor
曲琪环
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Northeast Agricultural University
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Northeast Agricultural University
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Application filed by Northeast Agricultural University filed Critical Northeast Agricultural University
Priority to CN200610009904.6A priority Critical patent/CN101049109A/en
Publication of CN101049109A publication Critical patent/CN101049109A/en
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  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

一种无公害种子包衣剂及其制备方法,它涉及一种种子包衣剂及其制备方法。它解决了现有种子包衣剂中剧毒物质严重危害人体健康、污染环境的缺陷。无公害种子包衣剂按质量百分比由0.001~31%的添加剂和余量是体积浓度为0.1~3%的壳聚糖和/或低聚壳聚糖溶液制成;其中添加剂由昆虫抗菌肽、腺苷酸、根瘤菌或过氧化氢酶中的一种或几种组成。其制备方法:(一)将壳聚糖和/或低聚壳聚糖放入酸溶液中,制成壳聚糖和/或低聚壳聚糖溶液;(二)加入添加剂,搅拌均匀,即得到无公害种子包衣剂。本发明原料为天然、可再生资源,综合利用成本低;制备出的种子包衣剂对人畜、环境无不良影响。应用本发明的植物在生长过程中病虫害减少60%以上,生长速度增快5~10%,产量提高10~15%。A pollution-free seed coating agent and a preparation method thereof, which relate to a seed coating agent and a preparation method thereof. It solves the defects that the highly toxic substances in the existing seed coating agents seriously endanger human health and pollute the environment. The pollution-free seed coating agent is made of 0.001-31% of additives and the balance is 0.1-3% volume concentration of chitosan and/or chitosan oligosaccharide solution according to the mass percentage; wherein the additives are insect antibacterial peptides, One or more of adenosine, rhizobia or catalase. Its preparation method: (1) putting chitosan and/or chitosan oligosaccharide into an acid solution to make chitosan and/or chitosan oligosaccharide solution; (2) adding additives and stirring evenly, namely Obtain the pollution-free seed coating agent. The raw materials of the invention are natural and renewable resources, and the comprehensive utilization cost is low; the prepared seed coating agent has no adverse effects on humans, animals and the environment. During the growth process of the plants applied with the invention, the pests and diseases are reduced by more than 60%, the growth speed is increased by 5-10%, and the yield is increased by 10-15%.

Description

A kind of nuisance free coating agent for seeds and preparation method thereof
Technical field
The present invention relates to a kind of coating agent for seed and preparation method thereof.
Background technology
Existing coating agent for seed is because of reaching the purpose of prevention and elimination of disease and pests, often make by the material that severe toxicity is arranged, its residue is very harmful to people and animals, and use this type of coating agent for seed for a long time and cause the drug resistance of damage by disease and insect to strengthen, also contaminated soil and underground water source have also destroyed the normal flora in the soil simultaneously.
Summary of the invention
The present invention is in order to solve the defective of extremely toxic substance serious harm health, contaminated environment in the existing coating agent for seed, and a kind of nuisance free coating agent for seeds and preparation method thereof is provided.Nuisance free coating agent for seeds is that volumetric concentration is that 0.1~3% shitosan and/or oligopolymerization chitosan sugar juice are made by 0.001~31% additive and surplus by mass percentage; Wherein additive is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.Nuisance free coating agent for seeds is prepared by following step: (one) puts into volumetric concentration by 1: 32.3~999 volume ratio with shitosan and/or chitosan oligomer is 0.1~5% acid solution, makes shitosan and/or oligopolymerization chitosan sugar juice; (2) add the additive that accounts for nuisance free coating agent for seeds solution gross mass 0.001~31%, stir, promptly obtain nuisance free coating agent for seeds; Wherein the additive in the step (two) is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.
Composition in the additive of the present invention extracts in animal, plant or microbial body, and no extremely toxic substance and heavy metal in the raw material are natural, renewable resources, and raw material sources extensively, be easy to get.Technology of the present invention is simple, and is workable, and the comprehensive utilization cost is low; The coating agent for seed of preparing has no adverse effects to people and animals, environment, is a kind of nuisanceless, Green Product.The coating agent for seed that the present invention prepares is easy to use, can directly spray or soak pending seed, just can use after drying; Nuisance free coating agent for seeds of the present invention also can be used for spraying or soaking root, stem and the leaf of vegetables except that spraying or soak the seed, and effect is desirable equally; But also can earlier seed be put into concentration is that the herb liquid that 0.01~1g/mL has a disease and insect resistance effect soaks 10min~4h, and seed dries the back and sprays or soak nuisance free coating agent for seeds of the present invention.Use seed of the present invention and common blank sample seed compares experiment under equivalent environment, using plant of the present invention damage by disease and insect in process of growth reduces more than 60%, growth rate speeds 5~10%, branches and leaves are luxuriant, completely filled fruit, beautiful in colour, mouthfeel good, quality is apparently higher than control group, output also improves 10~15%.
Embodiment
Embodiment one: the present embodiment nuisance free coating agent for seeds is that volumetric concentration is that 0.1~3% shitosan and/or oligopolymerization chitosan sugar juice are made by 0.001~31% additive and surplus by mass percentage; Wherein additive is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.
Shitosan in the present embodiment nuisance free coating agent for seeds and chitosan oligomer can with the heavy metal element chelating in soil or the water, alleviate the absorption of plant to heavy metal element, can avoid a large amount of depositions of heavy metal in human body indirectly.
Embodiment two: the difference of present embodiment and embodiment one is: additive is that the catalase of 500~3000U/mg is formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8% and 0.0025~3%.Other is identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is: additive is the adenylate that accounts for nuisance free coating agent for seeds gross mass 0.002~5%.Other is identical with embodiment one.
The adenylate that present embodiment adds can be regulated plant growth rate, promotes volume increase and the precocity of crops.
Embodiment four: the difference of present embodiment and embodiment one is: additive is that the enzyme work that accounts for nuisance free coating agent for seeds gross mass 0.0025~3% is the catalase of 500~3000U/mg.Other is identical with embodiment one.
The adding catalase helps removing harmful substance and the additional soil nutrient in the growing process in the present embodiment.
Embodiment five: the difference of present embodiment and embodiment one is: additive is the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%.Other is identical with embodiment one.
The insect antimicrobial peptide that adds in the present embodiment helps promoting plant growing and prevention and elimination of disease and pests (comprising bacterium, virus and harmful fungoid), and long-time use also is difficult for developing immunity to drugs, and is that small molecular protein is easy to decompose, absorb.
Embodiment six: the difference of present embodiment and embodiment one is: additive is made up of the adenylate of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8% and 0.002~5%.Other is identical with embodiment one.
Embodiment seven: the difference of present embodiment and embodiment one is: additive is that the catalase of 500~3000U/mg and 0.002~5% adenylate are formed by the enzyme work that accounts for nuisance free coating agent for seeds gross mass 0.0025~3%.Other is identical with embodiment one.
Embodiment eight: the difference of present embodiment and embodiment one is: additive is the rhizobium that account for nuisance free coating agent for seeds gross mass 0.1~15%.Other is identical with embodiment one.
Add rhizobium in the present embodiment and help strengthening the nodulation and nitrogen fixation performance, reduce the nitrogenous fertilizer usage amount, the fertile soil of supporting strengthens disease-resistant crops, resistance, improves crops quality, increases output more than 10%.
Embodiment nine: the difference of present embodiment and embodiment one is: additive is made up of the rhizobium that account for nuisance free coating agent for seeds gross mass 0.1~15%, 0.001~8% insect antimicrobial peptide and 0.002~5% adenylate.Other is identical with embodiment one.
Embodiment ten: the difference of present embodiment and embodiment one is: additive is that the catalase of 500~3000U/mg is formed by the enzyme work of the rhizobium that account for nuisance free coating agent for seeds gross mass 0.1~15% and 0.0025~3%.Other is identical with embodiment one.
Embodiment 11: the difference of present embodiment and embodiment one is: rhizobium are rhizobium of bean, peanut rhizobium, rhizobium melioti or herbage rhizobium; Effective bacterium number in every gram rhizobium 〉=200,000,000.Other is identical with embodiment one.
Soak the beans crop seed with the nuisance free coating agent for seeds that adds rhizobium of bean in the present embodiment, soak peanut seed with the nuisance free coating agent for seeds that adds peanut rhizobium in the present embodiment, soak alfalfa seed with the nuisance free coating agent for seeds that adds rhizobium melioti in the present embodiment, soak grass seed, crop yield 20~60% with the nuisance free coating agent for seeds that adds the herbage rhizobium in the present embodiment.
Embodiment 12: the difference of present embodiment and embodiment one is: additive is that catalase, 0.001~8% insect antimicrobial peptide and 0.002~5% the adenylate of 500~3000U/mg formed by the enzyme work that accounts for nuisance free coating agent for seeds gross mass 0.0025~3%.Other is identical with embodiment one.
Embodiment 13: the difference of present embodiment and embodiment one is: nuisance free coating agent for seeds is that volumetric concentration is that 1~2.5% chitosan solution is made by 0.1~25% additive and surplus by mass percentage.Other is identical with embodiment one.
Select for use chitosan solution to be of value to the release of antimicrobial component in the seed coating medicine in the present embodiment, have antibiotic preferably, antivirus action.Use its antibiotic, that the anti-virus ability ratio uses common coating agent for seed plant raising 25~35% of plant of present embodiment.
Embodiment 14: the difference of present embodiment and embodiment one is: nuisance free coating agent for seeds is that volumetric concentration is that 1~2.5% oligopolymerization chitosan sugar juice is made by 1~25% additive and surplus by mass percentage.Other is identical with embodiment one.
Select for use the oligopolymerization chitosan sugar juice to help the slow release of each composition in the seed coating medicine in the present embodiment, long duration of action, its additive effective time is than common coating agent for seed long 20~30%.
Embodiment 15: the difference of present embodiment and embodiment one is: nuisance free coating agent for seeds is that volumetric concentration is that 1~2.5% shitosan and chitosan oligomer mixed solution are made by 4~20% additive and surplus by mass percentage.Other is identical with embodiment one.
Select for use shitosan and chitosan oligomer mixed solution can improve antibiotic, the antivirus action of seed coating medicine in the present embodiment, can slowly discharge the composition in the seed coating medicine again.
Embodiment 16: the difference of present embodiment and embodiment one is: the deacetylation of shitosan is 70~90%.Other is identical with embodiment one.
Embodiment 17: present embodiment is carried out the preparation of nuisance free coating agent for seeds by following step: (one) puts into volumetric concentration by 1: 32.3~999 volume ratio with shitosan and/or chitosan oligomer is 0.1~5% acid solution, makes shitosan and/or oligopolymerization chitosan sugar juice; (2) add the additive that accounts for nuisance free coating agent for seeds solution gross mass 0.001~31%, stir, promptly obtain nuisance free coating agent for seeds; Wherein the additive in the step (two) is that in the catalase of 500~3000U/mg one or more are formed by the enzyme work of the insect antimicrobial peptide that accounts for nuisance free coating agent for seeds gross mass 0.001~8%, 0.002~5% adenylate, 0.1~15% rhizobium or 0.0025~3%.
The nuisance free coating agent for seeds that present embodiment makes soaked the field crop seed 10 minutes~7 days, can sow after the field crop seed dries.The nuisance free coating agent for seeds that present embodiment makes soaked the vegetables seed 10 minutes~7 days, can sow after the vegetables seed dries.
Embodiment 18: the difference of present embodiment and embodiment 17 is: the acid solution in the step () is organic acid soln or inorganic acid solution.Other is identical with embodiment 17.
Embodiment 19: the difference of present embodiment and embodiment 17 is: the acid solution in the step () is a glacial acetic acid solution.Other is identical with embodiment 17.

Claims (10)

1、一种无公害种子包衣剂,其特征在于无公害种子包衣剂按质量百分比由0.001~31%的添加剂和余量是体积浓度为0.1~3%的壳聚糖和/或低聚壳聚糖溶液制成;其中添加剂由占无公害种子包衣剂总质量0.001~8%的昆虫抗菌肽、0.002~5%的腺苷酸、0.1~15%的根瘤菌或0.0025~3%的酶活为500~3000U/mg的过氧化氢酶中的一种或几种组成。1. A pollution-free seed coating agent is characterized in that the pollution-free seed coating agent is composed of 0.001 to 31% additives and the balance by volume concentration of 0.1 to 3% chitosan and/or oligomeric Made from chitosan solution; wherein the additives consist of 0.001 to 8% of insect antimicrobial peptides, 0.002 to 5% of adenosine, 0.1 to 15% of rhizobium or 0.0025 to 3% of the total mass of the pollution-free seed coating agent One or several kinds of catalase with an enzyme activity of 500-3000U/mg. 2、根据权利要求1所述的一种无公害种子包衣剂,其特征在于添加剂由占无公害种子包衣剂总质量0.001~8%的昆虫抗菌肽和0.0025~3%的酶活为500~3000U/mg的过氧化氢酶组成。2. A pollution-free seed coating agent according to claim 1, characterized in that the additive consists of 0.001-8% insect antimicrobial peptide and 0.0025-3% of the total mass of the pollution-free seed coating agent. ~3000U/mg catalase composition. 3、根据权利要求1所述的一种无公害种子包衣剂,其特征在于添加剂为占无公害种子包衣剂总质量0.002~5%的腺苷酸。3. The pollution-free seed coating agent according to claim 1, characterized in that the additive is adenosine acid accounting for 0.002-5% of the total mass of the pollution-free seed coating agent. 4、根据权利要求1所述的一种无公害种子包衣剂,其特征在于添加剂为占无公害种子包衣剂总质量0.0025~3%的酶活为500~3000U/mg的过氧化氢酶。4. A pollution-free seed coating agent according to claim 1, characterized in that the additive is catalase with an enzyme activity of 500-3000 U/mg accounting for 0.0025-3% of the total mass of the pollution-free seed coating agent . 5、根据权利要求1所述的一种无公害种子包衣剂,其特征在于添加剂由占无公害种子包衣剂总质量0.1~15%的根瘤菌、0.001~8%的昆虫抗菌肽和0.002~5%的腺苷酸组成。5. A pollution-free seed coating agent according to claim 1, characterized in that the additives consist of 0.1-15% rhizobium, 0.001-8% insect antimicrobial peptide and 0.002% of the total mass of the pollution-free seed coating agent. ~5% adenylic acid composition. 6、根据权利要求1所述的一种无公害种子包衣剂,其特征在于壳聚糖的脱乙酰度为70~90%。6. A pollution-free seed coating agent according to claim 1, characterized in that the degree of deacetylation of chitosan is 70-90%. 7、根据权利要求1所述的一种无公害种子包衣剂,其特征在于根瘤菌为大豆根瘤菌、花生根瘤菌、苜蓿根瘤菌或牧草根瘤菌;每克根瘤菌中有效菌数≥2亿个。7. A pollution-free seed coating agent according to claim 1, characterized in that the rhizobia are soybean rhizobia, peanut rhizobia, alfalfa rhizobium or forage rhizobia; the number of effective bacteria per gram of rhizobia is ≥2 billion. 8、一种无公害种子包衣剂的制备方法,其特征在于无公害种子包衣剂按下述步骤进行制备:(一)按1∶32.3~999的体积比将壳聚糖和/或低聚壳聚糖放入体积浓度为0.1~5%的酸溶液中,制成壳聚糖和/或低聚壳聚糖溶液;(二)加入占无公害种子包衣剂溶液总质量0.001~31%的添加剂,搅拌均匀,即得到无公害种子包衣剂;其中步骤(二)中的添加剂由占无公害种子包衣剂总质量0.001~8%的昆虫抗菌肽、0.002~5%的腺苷酸、0.1~15%的根瘤菌或0.0025~3%的酶活为500~3000U/mg的过氧化氢酶中的一种或几种组成。8. A preparation method of a pollution-free seed coating agent, characterized in that the pollution-free seed coating agent is prepared according to the following steps: (1) chitosan and/or low Polychitosan is put into the acid solution that volume concentration is 0.1~5%, makes chitosan and/or chitosan oligosaccharide solution; % additives, stirred evenly, promptly obtains the pollution-free seed coating agent; Wherein the additive in the step (two) is by accounting for the insect antimicrobial peptide of 0.001~8% of the total mass of the pollution-free seed coating agent, 0.002~5% adenosine acid, 0.1-15% rhizobia or 0.0025-3% catalase with an enzyme activity of 500-3000 U/mg or more. 9、根据权利要求8所述的一种无公害种子包衣剂的制备方法,其特征在于步骤(一)中的酸溶液为有机酸溶液或无机酸溶液。9. A method for preparing a pollution-free seed coating agent according to claim 8, characterized in that the acid solution in step (1) is an organic acid solution or an inorganic acid solution. 10、根据权利要求8所述的一种无公害种子包衣剂的制备方法,其特征在于步骤(一)中的酸溶液为冰醋酸溶液。10. The method for preparing a pollution-free seed coating agent according to claim 8, characterized in that the acid solution in step (1) is a glacial acetic acid solution.
CN200610009904.6A 2006-04-05 2006-04-05 Nuisance free coating agent for seeds and preparation method Pending CN101049109A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102070368A (en) * 2010-08-23 2011-05-25 中国农业大学 Fulvic acid and rhizobium biological type leguminous plant seed coating agent and preparation method thereof
CN101591203B (en) * 2008-05-30 2013-01-09 山东银香伟业生物工程有限公司 Organic immunofertilizer preparation method
CN104016815A (en) * 2014-06-20 2014-09-03 山东省花生研究所 Peanut seed coating agent
CN104839222A (en) * 2015-04-10 2015-08-19 韦永梁 Cowpea seed treatment agent and treatment method thereof
CN105191991A (en) * 2014-06-27 2015-12-30 哈尔滨归复记生物科技有限公司 Biological seed and root soaking preparation and preparation method thereof
CN106211857A (en) * 2011-09-08 2016-12-14 诺维信生物农业公司 Method for treating seeds and compositions
CN109673683A (en) * 2018-12-28 2019-04-26 北京城市之光生态环境有限公司 Antibacterial sustained release root-growing agent of one kind and preparation method thereof
CN112655825A (en) * 2020-12-23 2021-04-16 安徽希普生物科技有限公司 Feather protein peptide particle composition and preparation method thereof

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101591203B (en) * 2008-05-30 2013-01-09 山东银香伟业生物工程有限公司 Organic immunofertilizer preparation method
CN102070368A (en) * 2010-08-23 2011-05-25 中国农业大学 Fulvic acid and rhizobium biological type leguminous plant seed coating agent and preparation method thereof
CN106211857A (en) * 2011-09-08 2016-12-14 诺维信生物农业公司 Method for treating seeds and compositions
CN104016815A (en) * 2014-06-20 2014-09-03 山东省花生研究所 Peanut seed coating agent
CN105191991A (en) * 2014-06-27 2015-12-30 哈尔滨归复记生物科技有限公司 Biological seed and root soaking preparation and preparation method thereof
CN104839222A (en) * 2015-04-10 2015-08-19 韦永梁 Cowpea seed treatment agent and treatment method thereof
CN109673683A (en) * 2018-12-28 2019-04-26 北京城市之光生态环境有限公司 Antibacterial sustained release root-growing agent of one kind and preparation method thereof
CN112655825A (en) * 2020-12-23 2021-04-16 安徽希普生物科技有限公司 Feather protein peptide particle composition and preparation method thereof

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