CN101445496A - Method for preparing fucoxanthine by using supercritical CO2 technology - Google Patents
Method for preparing fucoxanthine by using supercritical CO2 technology Download PDFInfo
- Publication number
- CN101445496A CN101445496A CNA2008101682835A CN200810168283A CN101445496A CN 101445496 A CN101445496 A CN 101445496A CN A2008101682835 A CNA2008101682835 A CN A2008101682835A CN 200810168283 A CN200810168283 A CN 200810168283A CN 101445496 A CN101445496 A CN 101445496A
- Authority
- CN
- China
- Prior art keywords
- fucoxanthine
- supercritical
- tangle
- extract
- sea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000005516 engineering process Methods 0.000 title abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000284 extract Substances 0.000 claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229930002868 chlorophyll a Natural products 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- -1 carotenoid compounds Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 125000001033 ether group Chemical group 0.000 description 1
- SJWWTRQNNRNTPU-ABBNZJFMSA-N fucoxanthin Chemical compound C[C@@]1(O)C[C@@H](OC(=O)C)CC(C)(C)C1=C=C\C(C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)C(=O)C[C@]1(C(C[C@H](O)C2)(C)C)[C@]2(C)O1 SJWWTRQNNRNTPU-ABBNZJFMSA-N 0.000 description 1
- AQLRNQCFQNNMJA-UHFFFAOYSA-N fucoxanthin Natural products CC(=O)OC1CC(C)(C)C(=C=CC(=CC=CC(=CC=CC=C(/C)C=CC=C(/C)C(=O)CC23OC2(C)CC(O)CC3(C)C)C)CO)C(C)(O)C1 AQLRNQCFQNNMJA-UHFFFAOYSA-N 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a method for preparing fucoxanthine by using supercritical CO2 extractive technology. The fucoxanthine in the method takes sea tangle and rhizoid of the sea tangle as raw materials and ethanol as entrainer, and is prepared by the method of supercritical CO2 extraction. The fucoxanthine extracted by the method has high product purity, low production cost and little organic solvent dosage, and is more environment-friendly and safer.
Description
Technical field:
The present invention relates to a kind of supercritical CO of using
2Technology is extracted the method for fucoxanthine.
Technical background:
Fucoxanthine (fucoxanthin) is a kind of carotenoid compounds that exists in brown alga.It has effects such as anticancer, fat-reducing, anti-oxidant, protection cellular free radical damage, has no side effect.
Fucoxanthine is present in the brown alga, and sea-tangle is an a kind of important economy plantation brown alga, and world's annual production reaches 3,000,000 tons, and the output of China has surpassed half, and sea-tangle is a kind of source of fabulous fucoxanthine.
The preparation method of traditional fucoxanthine uses the solvent extraction marine alga, and common solvent is ether, ethyl acetate, acetone, dimethyl sulfoxide (DMSO) etc. or its mixture, but expensive higher because of solvent consumption, and the dissolvent residual problem is arranged.
Summary of the invention
The purpose of this invention is to provide a kind of method of extracting fucoxanthine, reduce production costs, and improve its purity.The fucoxanthine that is extracted not only can be applicable to protective foods, also can reach the requirement of producing pharmaceutical prod.
Adopt supercritical CO
2Technology can realize purpose of the present invention, and its technological process is as follows:
A) preheating: powdery sea-tangle, sea-tangle rhizoid are added in the extractor, be preheating to 30-50 ℃;
B) extract: with the CO of 10-25L/h
2Flow, the ethanol entrainment agent of adding 5-25% (weight ratio), at 30-50 ℃, about 25Mpa pressure extracts 2-3h down;
C) separate: extracting solution separates in separator under 20-40 ℃ of conditions at about 5Mpa, reclaims CO
2
D) extracting solution adds vacuum tank, and at 40-60 ℃, 0.04-0.1Mpa reclaims ethanol down, obtains 5-20% fucoxanthine.
E) said extracted thing (fucoxanthine) is put into vacuum drying oven, dry under 0.02-0.08Mpa condition at 40-60 ℃, dried extract packing is finished product.
With supercritical CO of the present invention
2The fucoxanthine that technology is produced has following advantage:
A) product purity height is functional strong;
B) not only can produce protective foods, also can be applicable to the exploitation of medical supplies;
C) production process CO
2Nontoxic, recyclable utilization reduces the use of organic solvent, more Environmental Safety.
Embodiment
Embodiment 1
Get 1000g sea-tangle powder (40 orders, moisture 25%), add in the preheating can, be preheated to 40 ℃, CO
2Mix with 5% (weight ratio) ethanol entrainment agent and to be forced into about 25Mpa, CO
2Flow 10L/h, by extractor, extracting solution in separating tank 30 ℃, Separation and Recovery CO under about 5Mpa condition
2Extract 3h continuously.Extracting solution adds in the vacuum tank, at 40 ℃, reclaims solvent under the 0.04Mpa condition, gets the fucoxanthine extract, purity 20% (containing chlorophyll a, Chlorofucsin, marine alga lipid).The said extracted thing is put into inherent 40 ℃ of vacuum drying oven, and 0.02Mpa is dry down, and dry back extract is finished product.
Embodiment 2
Get 1000g sea-tangle rhizoid powder (40 orders, moisture 20%), add in the preheating can, be preheated to 50 ℃, CO
2Mix with 20% (weight ratio) ethanol entrainment agent and to be forced into about 25Mpa, CO
2Flow 15L/h, by extractor, extracting solution in separating tank 40 ℃, Separation and Recovery CO under about 5Mpa condition
2Extract 3h continuously.Extracting solution adds in the vacuum tank, at 60 ℃, reclaims solvent under the 0.1Mpa condition, gets the fucoxanthine extract, purity 5% (containing chlorophyll a, Chlorofucsin, marine alga lipid).The said extracted thing is put into inherent 60 ℃ of vacuum drying oven, and 0.07Mpa is dry down, and dry back extract is finished product.
Claims (2)
1. use supercritical CO for one kind
2Add the method that entrainment agent extracts fucoxanthine, it is characterized in that described method comprises following step:
A) preheating: powdery sea-tangle, sea-tangle rhizoid are added in the extractor, be preheating to 30-50 ℃;
B) extract: with the CO of 10-25L/h
2Flow, the ethanol entrainment agent of adding 5-25% (weight ratio), at 30-50 ℃, about 25Mpa pressure extracts 2-3h down;
C) separate: extracting solution separates in separator under 20-40 ℃ of conditions at about 5Mpa, reclaims CO
2
D) extracting solution adds vacuum tank, and at 40-60 ℃, 0.04-0.1Mpa reclaims ethanol down, obtains 5-20% fucoxanthine;
2. method according to claim 1 is characterized in that and described extract (fucoxanthine) can be put into vacuum drying oven, and is at 40-60 ℃, dry under 0.02-0.08Mpa condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101682835A CN101445496B (en) | 2008-10-10 | 2008-10-10 | Method for preparing fucoxanthine by using supercritical CO2 technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101682835A CN101445496B (en) | 2008-10-10 | 2008-10-10 | Method for preparing fucoxanthine by using supercritical CO2 technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101445496A true CN101445496A (en) | 2009-06-03 |
| CN101445496B CN101445496B (en) | 2011-06-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101682835A Active CN101445496B (en) | 2008-10-10 | 2008-10-10 | Method for preparing fucoxanthine by using supercritical CO2 technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101445496B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102336725A (en) * | 2011-06-10 | 2012-02-01 | 秦皇岛大惠生物技术有限公司 | Method for extracting fucoxanthine-containing concentrate, product obtained through method, and application of product |
| US8591825B2 (en) | 2011-05-18 | 2013-11-26 | Industrial Technology Research Institute | Extraction apparatus |
| CN105001184A (en) * | 2015-06-17 | 2015-10-28 | 宁波大学 | Pretreatment method of brown alga samples for fucoxanthin extraction |
| CN106983777A (en) * | 2017-04-21 | 2017-07-28 | 上海衡志贸易有限公司 | Brown alga pomegranate seed nutriment and preparation method thereof |
-
2008
- 2008-10-10 CN CN2008101682835A patent/CN101445496B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8591825B2 (en) | 2011-05-18 | 2013-11-26 | Industrial Technology Research Institute | Extraction apparatus |
| CN102336725A (en) * | 2011-06-10 | 2012-02-01 | 秦皇岛大惠生物技术有限公司 | Method for extracting fucoxanthine-containing concentrate, product obtained through method, and application of product |
| CN102336725B (en) * | 2011-06-10 | 2014-09-10 | 秦皇岛大惠生物技术有限公司 | Method for extracting fucoxanthine-containing concentrate, product obtained through method, and application of product |
| CN105001184A (en) * | 2015-06-17 | 2015-10-28 | 宁波大学 | Pretreatment method of brown alga samples for fucoxanthin extraction |
| CN105001184B (en) * | 2015-06-17 | 2017-07-21 | 宁波大学 | A kind of pre-treating method for extracting fucoxanthin brown alga sample |
| CN106983777A (en) * | 2017-04-21 | 2017-07-28 | 上海衡志贸易有限公司 | Brown alga pomegranate seed nutriment and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101445496B (en) | 2011-06-08 |
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Effective date of registration: 20110524 Address after: 100081 Room 518, South Building, building 10, a South Street, Zhongguancun, Beijing, Haidian District, Yinhai Applicant after: Beijing Leili United Ocean Biological Technology Co.,Ltd. Address before: 100081, South Building, No. 10, South Main Street, Beijing, Haidian District, Yinhai, 519, South District, Zhongguancun Applicant before: Tang Jie |