CN101700026A - A kind of bactericidal composition containing cyproconazole - Google Patents

Abstract

The invention discloses a bactericidal composition containing cyproconazole, wherein the weight ratio of cyproconazole to tricyclazole/isoprothiolane is 5: 1-1: 20, and the composition can be prepared into water dispersible granules, wettable powder, suspending agents, dispersible oil suspending agents, micro-emulsions or missible oil. The invention is used for preventing and treating rice blast, sheath blight and false smut of rice, has reasonable components, prevention and treatment effects, good bactericidal effect, enlarged prevention and treatment spectrum, can prevent and treat three diseases of rice blast, sheath blight and false smut of rice by one-time application, reduces the application times, has low application cost, has obvious synergistic effect on the activity and bactericidal effect of the rice blast, is not the simple superposition of the activities of the components, slows down the generation of resistance, has good safety on crops and meets the safety requirement of pesticide preparations.

Description

A kind of bactericidal composition that contains Cyproconazole

Technical field

The present invention relates to a kind of bactericidal composition, the bactericidal composition that particularly a kind of effective active composition is made up of Cyproconazole and tricyclazole or Isoprothiolane belongs to technical field of compounded pesticide.

Background technology

The English common name cyproconazole of Cyproconazole, molecular formula C ₁₅H ₁₈ClN ₃O, chemical name 2-(4-chlorphenyl)-3-cyclopropyl-1-(1H-1,2, the 4-triazol-1-yl) fourth-2-alcohol, belong to azoles fungicide, the mechanism of action is for suppressing the sterol demethylation, be used to prevent and treat cereal and coffee rust, cereal, fruit tree and grape powdery mildew, peanut, the beet tops pinta, apple scab and peanut white rot, have prevention and therapeutic action, to cereal crop (using 80g/ha), coffee (20-50g/ha), beet (40-60g/ha), Erysiphales on fruit tree and the grape (10g/ha), Uredinales, belong to the mould genus of spore, Rhynchosporium spp, Septoria, the Venturia bacterium is all effective, and it is efficient to rice sheath blight disease and rice green smut, and is active general to rice Pyricularia oryzae Cav bacterium, although Cyproconazole is as the outstanding kind newly developed in the triazole type agricultural chemicals, but pathogen is also very fast to the speed that triazole bactericidal agent produces resistance, and it is increasing to use dosage separately, and the dosage conference suppresses plant growth.Continuous use surpasses three times, cause probably that crop leaf diminishes, hardening, become fragile, poisoning situation such as blackening, will be the development need of science, the rational use of medicines therefore with the bactericide compounded of Cyproconazole and other mechanisms of action.

The English common name tricyclazole of tricyclazole, molecular formula C ₉H ₇N ₃S, chemical name 5-methyl isophthalic acid, 2, the 4-triazol (3,4-b) benzothiazole.Be a kind of stronger protectiveness triazole bactericidal agent of interior absorption that has, can absorbed, and be transported to each one of rice strain by rice root, stem, leaf rapidly.Tricyclazole antiscour power is strong, and the spray medicine is met the rain medicine that need not fill spray after one hour.Mainly be to suppress spore germination and adhere to spore to form; thereby stop germ to invade and reduce the generation of magnaporthe grisea spore effectively; can suppress appresorium formation effectively on rice strain surface; stop the mycelia people to invade; has the good preventing effect; but in rice strain body, the growth of mycelia is not had inhibitory action, but can suppress infecting again of germ; be used to prevent and treat rice blast; lasting period is long, and efficacy stability is to rice sheath blight disease; the false smut preventive effect is relatively poor; as protective fungicide; no therapeutic action, long-term a large amount of the use causes Pyricularia oryzae to rise serious to the resistance of tricyclazole.

The English common name isoprothiolane of Isoprothiolane, molecular formula C ₁₂H ₁₈O ₄S ₂, chemical name diisopropyl-1,3-dithiolane-a-phenyl malonic ester.Be a kind of efficient, systemic fungicide, rice blast is had special efficacy, relatively poor to rice sheath blight disease and false smut preventive effect.Rice plant accumulates on leaf texture after absorbing medicament, concentrates on cob and branch stalk especially, invades thereby suppress germ, hinders the germ lipid-metabolism, suppresses the germ growth, plays prevention and treatment.But national rice district still uses its main medicament as control rice blast so far widely for a long time after 1981, and the Pyricularia oryzae field resistance is serious day by day, causes the unit are consumption progressively strengthening, and the control cost constantly rises.

Rice blast, banded sclerotial blight, false smut are paddy rice common frdquently encountered disease evils, and it usually becomes crushing disaster.In actual use, Cyproconazole has very high bactericidal activity to rice sheath blight disease, false smut, but resistance rise year by year and dosage is big or control easily produces the defective of poisoning often.Tricyclazole and Isoprothiolane be as the rice blast efficient germicide, but have the serious day by day of resistance, and control efficiency descends, shortcomings such as use cost rising.

Summary of the invention

The object of the present invention is to provide a kind of component reasonable, control efficiency is remarkable, has synergistic effect, control is composed extensively, can be reduced spraying times and dosage, slow down the resistance generation of germ, reduce the control cost, the rice field bactericidal composition of crop safety to Cyproconazole, tricyclazole or Isoprothiolane.

Another object of the present invention is to provide above-mentioned composition to be used to prevent and treat the purposes of rice blast, banded sclerotial blight and false smut.

In order to overcome the defective of existing unitary agent, technical scheme of the present invention is to solve like this:

A kind of bactericidal composition that contains Cyproconazole comprises the first active component Cyproconazole, second active component tricyclazole or the Isoprothiolane; The weight ratio of first active component and second active component 5: 1～1: 20.The cumulant of first active component and second active component is 5%～90% of a described composition total weight, is preferably 20%～50%.

According to method known in those skilled in the art, the present invention can be mixed with agricultural when containing the rice field bactericidal composition practical application of Cyproconazole and go up any formulation that allows, but preparation formulation is water dispersible granules, wetting powder, suspending agent dispersed oil suspending agent, microemulsion or missible oil.

For water dispersible granules, those skilled in the art are familiar with the use corresponding adjuvants and finish the present invention very much.In dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; In disintegrant such as ammonium sulfate, urea, sucrose, the glucose one or more; In binding agent such as diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family one or more; In filler such as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, the potter's clay one or more.

To wetting powder, spendable auxiliary agent has: in dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; Filler such as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.

To suspending agent, spendable auxiliary agent has: among dispersant such as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate (dispersing agent NNO), the TERSPERSE 2425 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt) one or more; Alkyl phenol formaldehyde resin polyoxyethylene ether), farming breast 2201, this dish-60# (common name: Span60), Tween-60 # (common name: the polyoxyethylene sorbitan stearate), agricultural newborn 1601# (common name: triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), among the TERSPERSE 4894 (U.S. Huntsman Corporation product) one or more emulsifier such as Nongru-700 # (common name:; Among wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, the TERSPERSE 2500 (U.S. Huntsman Corporation product) one or more; In thickener such as xanthans, polyvinyl alcohol, bentonite, the aluminium-magnesium silicate one or more; In preservative such as formaldehyde, benzoic acid, the Sodium Benzoate one or more; In stabilizing agent such as epoxidized soybean oil, epoxychloropropane, the triphenyl phosphate one or more; Defoamer such as organic silicon defoamer; In antifreezing agent such as ethylene glycol, propane diols, glycerine, urea, inorganic salts such as the sodium chloride one or more.

But to the dispersed oil suspending agent, spendable auxiliary agent has: one or more in wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy base ether phosphate, lauryl alcohol polyethylene glycol oxide base ether sodium sulfate, neopelex, alkylnaphthalene sulfonate, lauryl sodium sulfate, alkylphenol polyoxyethylene formaldehyde condensation products succinate sodium sulfonate, the alkylphenol polyoxyethylene sulfosuccinate disodium; Among dispersant such as sodium lignin sulfonate, calcium lignosulfonate, alkyl naphthalene formaldehyde condensate sulfonates, polycarboxylate (TERSPERSE2700 that U.S. Huntsman Corporation produces), the TERSPERSE 2425 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt) one or more; In emulsifier such as calcium dodecyl benzene sulfonate, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene formaldehyde condensation products, alkylphenol-polyethenoxy polyethenoxy ether, APEO oleoyl carboxylic ester, alkyl phenol formaldehyde resin polyoxyethylene ether, Span60, polyoxyethylene sorbitan stearate, the triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer one or more; In thickener such as xanthans, carboxymethyl cellulose, white carbon, polyvinyl alcohol, bentonite, the aluminium-magnesium silicate one or more; In stabilizing agent such as epoxychloropropane, tributyl phosphate, triphenyl phosphite, epoxidized soybean oil, Sodium Benzoate, sodium sorbate, the formaldehyde one or more; Antifreezing agent such as ethylene glycol, propane diols, glycerine, urea, inorganic salts such as sodium chloride a kind of; Defoamer is the dimethicone defoamer, organic silicon modified by polyether defoamer or polyethers defoamer; Non-water system dispersion medium such as soybean oil, corn oil, cottonseed oil, palm oil, linseed oil, one or more in turpentine oil, rapeseed oil, corn oil, methylated vegetable oil such as the methyl oleate.

To microemulsion, spendable auxiliary agent has: emulsifier such as calcium dodecyl benzene sulfonate (agricultural newborn 500#), Nongru-700 # (common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agricultural newborn 2201#, this dish-60# (common name: Span60), Tween-60 # (common name: the polyoxyethylene sorbitan stearate), TX-10 (common name: octyl phenol polyoxy ethene (10) ether), farming breast 1601 (common name: triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), among agricultural newborn 600#, the agricultural newborn 400# one or more; In assistant for emulsifying agent such as methyl alcohol, isopropyl alcohol, n-butanol, the ethanol one or more; Solvent such as acetone, cyclohexanone, N-Methyl pyrrolidone, dimethyl formamide, dimethyl sulfoxide (DMSO), dimethylbenzene, toluene, solvent naphtha (trade mark: S-150, S-180, S-200) one or more; In stabilizing agent such as triphenyl phosphite, the epoxychloropropane one or more; Water is deionized water.

For missible oil, spendable auxiliary agent has: emulsifier such as calcium dodecyl benzene sulfonate (agricultural newborn 500#), Nongru-700 # (common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agricultural newborn 2201#, this dish-60# (common name: Span60), Tween-60 # (common name: the polyoxyethylene sorbitan stearate), TX-10 (common name: octyl phenol polyoxy ethene (10) ether), agricultural newborn 1601# (common name: triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), among agricultural newborn 600#, the agricultural newborn 400# one or more; In solvent such as dimethylbenzene, toluene, solvent naphtha (S-150, S-180, S-200), toluene, methyl alcohol, dimethyl formamide, N-Methyl pyrrolidone, cyclohexanone, acetone, the dimethyl sulfoxide (DMSO) one or more.

Component of the present invention is reasonable, control rice blast effect is remarkable, has synergistic effect, and a dispenser control rice blast, banded sclerotial blight and three kinds of diseases of false smut have improved the control efficiency of prevention and treatment, enlarge fungicidal spectrum, reduce spraying times and dosage, slow down the resistance generation of germ to Cyproconazole, tricyclazole and Isoprothiolane, reduce the effect of control cost,, meet the security requirement of pesticidal preparations the crop safety height.The present invention has the better prevention effect to rice blast, banded sclerotial blight and false smut.

Embodiment

In order to prevent and treat the diseases such as rice blast, banded sclerotial blight and false smut in the agricultural production, the inventor has carried out mutual composite synergy research with compositions such as Cyproconazole, tricyclazole, Isoprothiolanes, and concrete grammar is:

1) the former medicine of rice blast fungus (pyricularia oryzae) test medicine Cyproconazole adopts the former medicine that Shangyu, Zhejiang Province city chemical industry Co., Ltd of silver nation produces, the former medicine that the former medicine of Isoprothiolane adopts Sichuan Prov. Chemical Industry Research ﹠ Design Academy to produce, the former medicine that the former medicine of tricyclazole adopts Jiangsu Changqing Agricultural Chemistry Co., Ltd. to produce.Test method is with reference to " the agricultural industry criteria NY/T1156.1-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy are provided with following 5 variable concentrations gradients respectively: Cyproconazole is 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml; Tricyclazole, Isoprothiolane are; 1.25 μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml; Cyproconazole and tricyclazole or the Isoprothiolane binary mixture concentration gradient that is mixed is 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 40 μ g/ml, establishes the clear water contrast; Repeat 3 times.Adopt spore suspension to sprout method, with deionized water wash-out, filtration from the medium, centrifugal (1000r/min) 5min removes supernatant, adds deionized water, and is centrifugal again with cultured pathogen spore.At last with deionized water with the spore resuspension to every milliliter 1 * 10 ⁵Individual～1 * 10 ⁷Individual spore, and add 0.5% glucose solution.

, from the low concentration to the high concentration, draw soup 0.5mL successively and add respectively in the small test tube with pipette, draw the spore suspension 0.5mL for preparing then, soup and spore suspension mixed in equal amounts are shaken up; Getting above-mentioned mixed liquor with micro sample adding appliance drips on the concave slide, frame is put in the culture dish that has shoaling layer then, adding a cover preserves moisture is incubated in the incubator of preference temperature, when the blank spore germination rate reaches 90%, check the spore germination situation of respectively handling, calculate and suppress preventive effect relatively, obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

2) Rhizoctonia solani Kuhn (Rhizoctonia solani) test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy are provided with following 5 variable concentrations gradients respectively: Cyproconazole is 0.625 μ g/ml, 1.25 μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml; Tricyclazole, Isoprothiolane are 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 40 μ g/ml, 80 μ g/ml; Cyproconazole and tricyclazole/Isoprothiolane binary mixture concentration gradient that is mixed is 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 40 μ g/ml, establishes the clear water contrast; Repeat 3 times.Adopting the mycelial growth rate method, with cultured pathogen, is the card punch of 5cm with diameter under aseptic condition, cuts the bacterium cake from colony edge, with transfer needle the bacterium cake is connected on the culture medium flat plate of different drug concentrations, puts in 26 ℃ of incubators and cultivates 72h; Check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, the EC of each medicament is obtained in the linear regression analysis between the probit value by inhibiting rate and the logarithm value of series concentration ₅₀Value, and calculate relative coefficient of synergism.

3) rice green smut bacterium (Ustilaginoidea virens) test method is with reference to " the agricultural industry criteria NY/T1156.1-2006 of the People's Republic of China (PRC) ".At first Cyproconazole and tricyclazole, the agent of Isoprothiolane list and each Mixed Pharmacy all are provided with following 5 variable concentrations gradients: Cyproconazole is 0.1875 μ g/ml, 0.375 μ g/ml, 0.75 μ g/ml, 1.5 μ g/ml, 3 μ g/ml; Tricyclazole, Isoprothiolane are 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml; Cyproconazole and tricyclazole or the Isoprothiolane binary mixture concentration gradient that is mixed is 0.25 μ g/ml, 0.5 μ g/ml, 1 μ g/ml, 2 μ g/ml, 4 μ g/ml, establishes the clear water contrast; Repeat 3 times.

The Wadley method: estimate the synergistic effect of pharmacy mix according to coefficient of synergism (SR), both SR＜0.5 was an antagonism, and 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect.

The coefficient of synergism of mixture (SR value) calculates by following formula:

$X_1=\frac{P_A+P_B}{P_A/A+P_B/B}\×100$

In the formula: X ₁--the EC of-mixture ₅₀Theoretical value, unit be the milligram every liter (mg/L)

P _A--the percentage composition of A in the-mixture, unit are percentage (%)

P _B--the percentage composition of B in the-mixture, unit are percentage (%)

The EC of A in the A---mixture ₅₀Value, unit is every liter (mg/L) of milligram

The EC of B in the B---mixture ₅₀Value, unit is every liter (mg/L) of milligram

$\mathrm{SR}=\frac{X_1}{X_2}$ …………………………

In the formula:

The coefficient of synergism of SR---mixture;

X ₁---mixture EC ₅₀Theoretical value, unit is every liter (mg/L) of milligram;

X ₂---mixture EC ₅₀Measured value, unit are every liter (mg/L) of milligram.

Table 1 Cyproconazole and tricyclazole or the Isoprothiolane Toxicity Determination result of composition that be mixed to rice blast fungus

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						Cyproconazole (A)	??Y＝1.4968+2.0518X	??0.9984	??50.97	??35.0568～??80.3451	??——
Tricyclazole (B)	??Y＝3.6788+2.0439X	??0.9956	??4.43	??3.3664～??6.3661	??——
						Isoprothiolane (C)	??Y＝3.3128+1.8100X	??0.9993	??8.55	??7.3680～??10.1167	??——
??A+B??(10∶1)	??Y＝2.5096+1.7686X	??0.9990	??25.59	??19.7164～??34.7237	??1.02
						??A+B??(5∶1)	??Y＝3.0423+1.8135X	??0.9957	??12.01	??8.0078～??20.9992	??1.54
??A+B??(2.5∶1)	??Y＝3.1032+2.0827X	??0.9980	??8.14	??6.4070～??10.9112	??1.57
						??A+B??(1∶1)	??Y＝3.6138+1.9747X	??0.9992	??5.03	??4.3979～??5.8679	??1.62
??A+B??(1∶2.5)	??Y＝3.8208+2.1738X	??0.9961	??3.49	??2.7811～??4.6395	??1.72
						??A+B??(1∶5)	??Y＝4.1117+1.8932X	??0.9994	??2.95	??2.6921～??3.2450	??1.77

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						??A+B??(1∶10)	??Y＝4.0617+1.9496X	??0.9992	??3.03	??2.7281～??3.4007	??1.59
??A+B??(1∶20)	??Y＝4.0557+1.9587X	??0.9982	??3.03	??2.5949～??3.6446	??1.53
						??A+B??(1∶40)	??Y＝3.8218+1.9688X	??0.9995	??3.97	??3.6423～??4.3515	??1.14
??A+C??(10∶1)	??Y＝3.0553+1.3016X	??0.9984	??31.20	??21.7895～??48.5859	??1.13
						??A+C??(5∶1)	??Y＝2.6434+1.9069X	??0.9958	??17.21	??11.0358～??31.7219	??1.62
??A+C??(2.5∶1)	??Y＝3.0988+1.7249X	??0.9946	??12.65	??8.0161～??24.4097	??1.67
						??A+C??(1∶1)	??Y＝2.6945+2.4616X	??0.9984	??8.65	??7.0068～??11.0766	??1.68
??A+C??(1∶2.5)	??Y＝3.4343+1.9030X	??0.9976	??6.65	??5.2089～??9.0026	??1.69
						??A+C??(1∶5)	??Y＝3.5531+1.9358X	??0.9996	??5.59	??5.0928～??6.1849	??1.78
??A+C??(1∶10)	??Y＝3.5436+1.9769X	??0.9996	??5.45	??4.9703～??6.0313	??1.70

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						??A+C??(1∶20)	??Y＝3.5226+1.9561X	??0.9998	??5.69	??5.3220～??6.1129	??1.56
??A+C??(1∶40)	??Y＝3.0297+2.2289X	??0.9971	??7.66	??5.9061～??10.5870	??1.14

Table 2 Cyproconazole and tricyclazole or the Isoprothiolane Toxicity Determination result of composition that be mixed to Rhizoctonia solani Kuhn

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						Cyproconazole (A)	??Y＝4.3858+2.3224X	??0.9986	??1.84	??1.6573～??2.0590	??——
Tricyclazole (B)	??Y＝2.7082+1.4199X	??0.9984	??41.11	??28.7499～??63.5088	??——
						Isoprothiolane (C)	??Y＝1.8314+1.8894X	??0.9901	??47.54	??20.5485～??193.8347	??——
??A+B??(10∶1)	??Y＝3.6955+2.1476X	??0.9965	??4.05	??3.2064～??5.4429	??0.50
						??A+B??(5∶1)	??Y＝3.7769+2.1535X	??0.9943	??3.70	??2.7980～??5.3769	??0.59
??A+B??(2.5∶1)	??Y＝3.8109+2.1460X	??0.9949	??3.58	??2.7627～??5.0373	??0.71

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						??A+B??(1∶1)	??Y＝3.8577+1.9755X	??0.9981	??3.79	??3.1916～??4.6362	??0.93
??A+B??(1∶2.5)	??Y＝3.8158+1.8477X	??0.9928	??4.37	??3.8760～??7.2406	??1.32
						??A+B??(1∶5)	??Y＝3.6344+1.7761X	??0.9991	??5.87	??5.0347～??7.0081	??1.54
??A+B??(1∶10)	??Y＝3.1044+2.0362X	??0.9979	??8.53	??6.6117～??11.6845	??1.64
						??A+B??(1∶20)	??Y＝2.3982+2.1872X	??0.9989	??15.47	??12.5018～??19.7968	??1.32
??A+B??(1∶40)	??Y＝2.9389+1.5293X	??0.9892	??22.27	??10.5831～??81.8377	??1.21
						??A+C??(10∶1)	??Y＝3.6779+2.3796X	??0.9984	??3.59	??3.1018～??4.2623	??0.56
??A+C??(5∶1)	??Y＝3.8214+2.2677X	??0.9958	??3.31	??2.6537～??4.3674	??0.66
						??A+C??(2.5∶1)	??Y＝4.0966+1.7770X	??0.9951	??3.22	??2.4868～??4.5382	??0.79
??A+C??(1∶1)	??Y＝4.0394+1.8092X	??0.9981	??3.40	??2.8646～??4.1553	??1.04

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						??A+C??(1∶2.5)	??Y＝3.9002+1.8273X	??0.9847	??4.00	??2.5144～??8.8752	??1.47
??A+C??(1∶5)	??Y＝3.5764+1.8144X	??0.9979	??6.09	??4.8553～??8.0425	??1.52
						??A+C??(1∶10)	??Y＝3.0810+1.9882X	??0.9941	??9.23	??6.0874～??16.7769	??1.58
??A+C??(1∶20)	??Y＝2.4959+2.0885X	??0.9970	??15.81	??11.2316～??24.4504	??1.38
						??A+C??(1∶40)	??Y＝2.1866+2.0045X	??0.9978	??25.32	??17.5737～??40.0700	??1.17

Table 3 Cyproconazole and tricyclazole or Isoprothiolane are mixed combination to the Toxicity Determination result of rice green smut bacterium

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						Cyproconazole (A)	??Y＝5.1723+2.0268X	??0.9972	??0.82	??0.7058～??0.9509	??——
Tricyclazole (B)	??Y＝1.9085+1.8723X	??0.9991	??44.78	??34.1482～??61.2064	??——
						Isoprothiolane (C)	??Y＝2.1482+1.5526X	??0.9969	??68.68	??41.5793～??131.0130	??——

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						??A+B??(10∶1)	??Y＝4.2637+2.1887X	??0.9967	??2.17	??1.8266～??2.6581	??0.41
??A+B??(5∶1)	??Y＝4.9226+2.1887X	??0.9966	??1.08	??0.9334～??1.2651	??0.91
						??A+B??(2.5∶1)	??Y＝5.0540+1.8506X	??0.9998	??0.93	??0.9078～??0.9629	??1.23
??A+B??(1∶1)	??Y＝4.8219+1.8464X	??0.9982	??1.25	??1.1106～??1.4115	??1.29
						??A+B??(1∶2.5)	??Y＝4.3980+2.1327X	??0.9983	??1.91	??1.6931～??2.1974	??1.43
??A+B??(1∶5)	??Y＝4.2067+1.7602X	??0.9939	??2.82	??2.1576～??4.0397	??1.60
						??A+B??(1∶10)	??Y＝3.5298+1.9938X	??0.9985	??5.46	??4.5574～??6.7640	??1.40
??A+B??(1∶20)	??Y＝3.0245+2.0190X	??0.9991	??9.52	??8.0178～??11.5688	??1.32
						??A+B??(1∶40)	??Y＝2.2697+2.0655X	??0.9995	??20.98	??17.8838～??25.0252	??0.93
??A+C??(10∶1)	??Y＝4.5753+1.8506X	??0.9958	??1.69	??1.4029～??2.1153	??0.53

Medicament	Virulence regression equation	Correlation (r)	??EC 50、??(μg/ml)	??EC 5095% confidence interval	Coefficient of synergism SR)
						??A+C??(5∶1)	??Y＝4.8159+1.9265X	??0.9956	??1.26	??1.0378～??1.5162	??0.78
??A+C??(2.5∶1)	??Y＝4.8662+1.9858X	??0.9950	??1.17	??0.9616～??1.4330	??0.97
						??A+C??(1∶1)	??Y＝4.7988+1.9763X	??0.9971	??1.26	??1.0897～??1.4801	??1.29
??A+C??(1∶2.5)	??Y＝4.3343+2.3743X	??0.9942	??1.90	??1.5572～??2.4325	??1.47
						??A+C??(1∶5)	??Y＝4.1344+1.8213X	??0.9914	??2.99	??2.1560～??4.7088	??1.55
??A+C??(1∶10)	??Y＝3.8180+1.8641X	??0.9986	??4.31	??3.6826～??5.1638	??1.87
						??A+C??(1∶20)	??Y＝3.2569+1.8641X	??0.9987	??8.61	??7.0094～??10.9829	??1.61
??A+C??(1∶40)	??Y＝1.8809+2.3419X	??0.9985	??21.47	??16.2091～??30.0491	??1.06

From table 1-3 as can be seen, Cyproconazole and tricyclazole or Isoprothiolane are composite, and weight ratio is 5: 1～1: 20, and rice blast fungus is had synergistic effect, is part synergy, part summation action to Rhizoctonia solani Kuhn and rice false smut.

Below in conjunction with embodiment content of the present invention is described further.

Embodiment 1:

Take by weighing 20% Cyproconazole, 40% tricyclazole, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 3% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% diatomite, 5% glucose, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 60% Cyproconazole tricyclazole water dispersible granule.This prescription is used to prevent and treat rice blast, and by 3500 times of dilute with water sprayings, 15 days preventive effect is 95.4% behind the medicine.10% Cyproconazole suspending agent is pressed 2000 times by 1000 times and 75% tricyclazole wettable powder, the use that uses the same method, and 14 days preventive effect is respectively to be 73.2% and 85.6% behind the medicine.

Embodiment 2

Take by weighing 10% Cyproconazole, 50% tricyclazole, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 1%K-12 (lauryl sodium sulfate), 1% carboxymethyl cellulose, 3% urea, diatomite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 60% Cyproconazole tricyclazole water dispersible granule.This prescription is used to prevent and treat rice blast, and by 3000 times of dilute with water sprayings, 14 days preventive effect is 96.1% behind the medicine.10% Cyproconazole suspending agent is pressed 2000 times by 1000 times and 75% tricyclazole wettable powder, the use that uses the same method, and 14 days preventive effect is respectively to be 68.5% and 84.8% behind the medicine.

Embodiment 3:

Take by weighing 5% Cyproconazole, 75% tricyclazole, 4%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% corn starch, 5% sulphur ammonium, attapulgite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 80% Cyproconazole tricyclazole water dispersible granule.This prescription is used to prevent and treat rice blast, and by 3000 times of dilute with water sprayings, 14 days preventive effect is 93.6% behind the medicine.10% Cyproconazole suspending agent is pressed 2000 times by 1000 times and 75% tricyclazole wettable powder, the use that uses the same method, and 14 days preventive effect is respectively to be 72.8% and 84.2% behind the medicine.

Embodiment 4:

Take by weighing 4% Cyproconazole, 40% Isoprothiolane, 4%Morwet D-425 (alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% corn starch, 5% sucrose, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 44% Cyproconazole 'Daowenling ' water dispersible granule.This prescription is used to prevent and treat rice blast, and by 1500 times of dilute with water sprayings, 14 days preventive effect is 92.8% behind the medicine.10% Cyproconazole suspending agent is pressed 800 times by 1000 times and 40% Isoprothiolane wetting powder, the use that uses the same method, and 14 days preventive effect is respectively to be 69.2% and 82.6% behind the medicine.

Embodiment 5

Take by weighing 10% Cyproconazole, 25% Isoprothiolane, 5% calcium lignosulfonate, 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 5% white carbon black, kaolin and add to 100% weight portion.Above-mentioned raw materials makes 35% Cyproconazole Isoprothiolane wetting powder through mixing behind the comminution by gas stream.This prescription is used to prevent and treat rice blast, and by 2000 times of dilute with water sprayings, 14 days preventive effect is 91.6% behind the medicine.10% Cyproconazole suspending agent is pressed 800 times by 1000 times and 40% Isoprothiolane wetting powder, the use that uses the same method, and 14 days preventive effect is respectively to be 71.6% and 85.3% behind the medicine.

Embodiment 6

Take by weighing 2% Cyproconazole, 40% Isoprothiolane, 4% calcium lignosulfonate, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% white carbon black, fine particle calcium carbonate adds to 100% weight portion.Above-mentioned raw materials makes 42% Cyproconazole Isoprothiolane wetting powder through mixing behind the comminution by gas stream.This prescription is used to prevent and treat rice blast, and by 1000 times of dilute with water sprayings, 7 days preventive effect is 92.5% behind the medicine.10% Cyproconazole suspending agent is pressed 800 times by 1000 times and 40% Isoprothiolane missible oil, the use that uses the same method, and 14 days preventive effect is respectively to be 68.2% and 84.2% behind the medicine.

Embodiment 7

Take by weighing 10% Cyproconazole, 25% tricyclazole, 3%TERSPERSE 4894 (U.S. Huntsman Corporation product), 1%TERSPERSE 2500 (U.S. Huntsman Corporation product), 2% dispersing agent NNO, 0.3% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), 1% epoxychloropropane, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with the Cyproconazole tricyclazole suspending agent that makes 35% behind the sand mill sand milling.This prescription is used to prevent and treat rice blast, false smut, and by 2000 times of dilute with water sprayings, 14 days rice blast, false smut preventive effect are respectively 90.2%, 91.34% behind the medicine.10% Cyproconazole suspending agent is pressed 1000 times by 1000 times and 40% tricyclazole suspending agent, use uses the same method, 14 days preventive effect behind the medicine, 10% Cyproconazole suspending agent to rice blast and false smut be respectively for 64.3% and 88.8%, 40% tricyclazole suspending agent be respectively to be 84.3% and 63.8% to rice blast and false smut.Control rice blast synergistic effect is obvious after Cyproconazole and the tricyclazole compounded combination, and preventive effect obviously is better than single agent, and a drug is prevented and treated multiple diseases, and effective ingredient consumption obviously reduces, to crop safety.

Embodiment 8

Take by weighing 10% Cyproconazole, 20% tricyclazole, 4%TERSPERSE 4894 (U.S. Huntsman Corporation product), 1.5%TERSPERSE 2500 (U.S. Huntsman Corporation product), 2%TERSPERSE 2425 (U.S. Huntsman Corporation product), 0.2% aluminium-magnesium silicate, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), 2% triphenyl phosphate, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with the Cyproconazole tricyclazole suspending agent that makes 30% behind the sand mill sand milling.This prescription is used to prevent and treat rice sheath blight disease, rice blast, and by 1700 times of dilute with water sprayings, 14 days rice sheath blight disease, rice blast preventive effect are respectively 95.53%, 91.34% behind the medicine.10% Cyproconazole suspending agent is pressed 1000 times by 1000 times and 40% tricyclazole suspending agent, use uses the same method, 14 days preventive effect behind the medicine, 10% Cyproconazole suspending agent to banded sclerotial blight and rice blast be respectively for 92.3% and 64.5%, 40% tricyclazole suspending agent be respectively to be 62.3% and 83.8% to banded sclerotial blight and rice blast.

Embodiment 9

Take by weighing 4% Cyproconazole, 28% Isoprothiolane, 5%TERSPERSE 4894 (U.S. Huntsman Corporation product), 2%TERSPERSE 2500 (U.S. Huntsman Corporation product), 3%TERSPERSE2425 (U.S. Huntsman Corporation product), 0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), 1% epoxychloropropane, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with the Cyproconazole Isoprothiolane suspending agent that makes 32% behind the sand mill sand milling.This prescription is used to prevent and treat rice green smut, rice blast, and by 2000 times of dilute with water sprayings, 14 days rice green smut, rice blast preventive effect are respectively 91.52%, 93.38% behind the medicine.10% Cyproconazole suspending agent is pressed 1000 times by 1000 times and 40% tricyclazole suspending agent, use uses the same method, 14 days preventive effect behind the medicine, 10% Cyproconazole suspending agent to false smut and rice blast be respectively for 87.3% and 65.7%, 40% Isoprothiolane missible oil be respectively to be 67.3% and 84.4% to false smut and rice blast.

Embodiment 10

Take by weighing 5% cyproconazole, 25% tricyclazole, dispersant 3% alkylnaphthalene sulfonate (TERSPERSE2425), emulsifier 2% Span60 (this dish-60#), 2% polyoxyethylene sorbitan stearate (Tween-60 #), wetting agent 1% lauryl alcohol polyethylene glycol oxide base ether sodium sulfate, thickener 2% bentonite, antifreezing agent 5% ethylene glycol, defoamer 0.2% dimethicone defoamer, stabilizing agent 1% epoxychloropropane, non-water system dispersion medium soybean oil adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, but promptly makes 30% cyproconazole tricyclazole dispersed oil suspending agent with sand mill sand milling to particle diameter less than 5 microns.This prescription is used to prevent and treat rice blast, hold concurrently anti-rice sheath blight disease, false smut, by 1500 times of dilute with waters sprayings, behind the medicine 14 days be 92.4% to the rice blast preventive effect, the preventive effect of hold concurrently anti-banded sclerotial blight, false smut is respectively 89.36%, 86.65%.10% Cyproconazole suspending agent is pressed 1000 times by 1000 times and 40% tricyclazole suspending agent, use uses the same method, behind the medicine 14 days the rice blast preventive effect is respectively 61.6% and 83.2%, the anti-rice sheath blight disease preventive effect of holding concurrently is respectively 86.62%, 67.22%, the anti-false smut preventive effect of holding concurrently is respectively 82.48%, 59.63%.

Embodiment 11

Take by weighing 20% cyproconazole, 10% Isoprothiolane, dispersant 2% polycarboxylate (TERSPERSE 2700 that U.S. Huntsman Corporation produces), emulsifier 2% Span60 (this dish-60#), 3% castor oil polyoxyethylene ether (BY-125), wetting agent 1% alkylphenol polyoxyethylene sulfosuccinate disodium, thickener 2% aluminium-magnesium silicate, antifreezing agent 4% propane diols, defoamer 0.2% organic silicon modified by polyether defoamer (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), stabilizing agent 10% epoxidized soybean oil, non-water system dispersion medium methyl oleate adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 20min, but promptly makes 30% cyproconazole Isoprothiolane dispersed oil suspending agent with sand mill sand milling to particle diameter less than 5 microns.This prescription is used to prevent and treat rice blast, and by 2500 times of dilute with water sprayings, 14 days preventive effect is 94.6% behind the medicine.10% Cyproconazole suspending agent is pressed 800 times by 1000 times and 40% Isoprothiolane missible oil, the use that uses the same method, and 14 days preventive effect is respectively to be 65.5% and 84.3% behind the medicine.

Embodiment 12

Take by weighing 10% Cyproconazole, 10% tricyclazole, 4%TX-10,5% Nongru-700 #, 6% agricultural newborn 500#, 4% agricultural newborn 1601#, 20% cyclohexanone, 15% dimethylbenzene, 5% n-butanol, 2% triphenyl phosphite, through dissolving fully and mixing, deionized water adds to 100% weight portion, makes 20% Cyproconazole tricyclazole microemulsion after the stirring.This prescription is used to prevent and treat rice blast, and by 1250 times of dilute with water sprayings, 14 days preventive effect is 94.2% behind the medicine.10% Cyproconazole suspending agent is pressed 2000 times by 1000 times and 75% tricyclazole wettable powder, the use that uses the same method, and 14 days preventive effect is respectively to be 65.7% and 85.5% behind the medicine.

Embodiment 13

Take by weighing 10% Cyproconazole, 30% Isoprothiolane, 5% Nongru-700 #, 6% agricultural newborn 500#, 6% agricultural newborn 600#, 20% dimethylbenzene, 10% methyl alcohol, 2% triphenyl phosphite, through dissolving fully and mixing, deionized water adds to 100% weight portion, makes 40% Cyproconazole Isoprothiolane microemulsion after the stirring.This prescription is used to prevent and treat rice blast, and by 10000 times of dilute with water sprayings, 14 days preventive effect is 93.8% behind the medicine.10% Cyproconazole suspending agent is pressed 800 times by 1000 times and 40% Isoprothiolane missible oil, the use that uses the same method, and 14 days preventive effect is respectively to be 67.5% and 84.7% behind the medicine.

Embodiment 14

Take by weighing 5% Cyproconazole, 15% tricyclazole, 4% agricultural newborn 500#, 2% agricultural newborn 600#, 3% agricultural newborn 2201#, 3% triphenyl phosphite, 20% dimethyl sulfoxide (DMSO), dimethylbenzene add to 100% weight portion.Above-mentioned raw materials makes 20% Cyproconazole tricyclazole missible oil after mix, stirring and dissolving is complete.This prescription is used to prevent and treat rice blast, and by 1000 times of dilute with water sprayings, 14 days preventive effect is 96.3% behind the medicine.10% Cyproconazole suspending agent is pressed 1000 times by 1000 times and 40% tricyclazole suspending agent, the use that uses the same method, and 14 days preventive effect is respectively to be 69.5% and 85.4% behind the medicine.

Embodiment 15:

Take by weighing 5% Cyproconazole, 40% Isoprothiolane, 5% agricultural newborn 500#, 2% agricultural newborn 400#, 3% Nongru-700 #, 1% epoxychloropropane, 5%N-methyl pyrrolidone, 5% cyclohexanone, dimethylbenzene add to 100% weight portion.Above-mentioned raw materials makes 45% Cyproconazole Isoprothiolane missible oil after mix, stirring and dissolving is complete.This prescription is used to prevent and treat rice blast, and by 1500 times of dilute with water sprayings, 14 days preventive effect is 95.3% behind the medicine.10% Cyproconazole suspending agent is pressed 800 times by 1000 times and 40% Isoprothiolane missible oil, the use that uses the same method, and 14 days preventive effect is respectively to be 64.3% and 85.6% behind the medicine.

From last embodiment as can be seen, after Cyproconazole and tricyclazole or Isoprothiolane are mixed according to a certain percentage, the control rice blast has significant synergistic effect, under certain application dosage, rice sheath blight disease and false smut had the better double effect of controlling, testing all processing does not all have obvious harmful effect to the growth of crop, and safety is better.

Comprehensively above-mentioned, the present invention is used to prevent and treat rice blast, banded sclerotial blight, false smut, component is reasonable, prevention adds therapeutic action, bactericidal effect is good, the expanding prevention spectrum, a dispenser control rice blast, banded sclerotial blight and three kinds of diseases of false smut, reduce the medication number of times, drug cost is low, and its activity and bactericidal effect to rice blast is not the simple superposition of each composition activity, but significant synergistic effect is arranged, slow down the generation of resistance, good to crop safety, meet the security requirement of pesticidal preparations.

Claims (6)

1. A bactericidal composition containing cyproconazole, characterized in that comprising: A) the first active ingredient cyproconazole; B) the second active ingredient tricyclazole or rice blastin; The weight ratio of the first active ingredient to the second active ingredient is 5:1˜1:20. 2. The fungicidal composition containing cyproconazole according to claim 1, characterized in that: the cumulative amount of the first active ingredient and the second active ingredient is 5%-90% of the total weight of the composition. 3. The fungicidal composition containing cyproconazole according to claim 2, characterized in that: the cumulative amount of the first active ingredient and the second active ingredient is 20%-50% of the total weight of the composition. 4. The fungicidal composition containing cyproconazole according to claim 1, characterized in that: said composition can be formulated into any dosage form allowed in agriculture. 5. the bactericidal composition containing cyproconazole according to claim 4, is characterized in that: said composition is water dispersible granule, wettable powder, suspending agent, dispersible oil suspending agent, microemulsion or emulsifiable concentrate dosage form. 6. the application of the bactericidal composition containing cyproconazole described in claim 1 in the control of rice blast, sheath blight, rice false smut of rice.