CN102357500B - Method for treating crop straw - Google Patents
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Abstract
本发明涉及一种农作物秸秆的处理方法。一种处理农作物秸秆的方法,其特征在于它包括如下步骤:1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成;2)秸秆原料的粉碎;3)发酵菌液A的配制;4)接种菌液A发酵:将发酵菌液A均匀喷散在粉碎后的秸秆上,堆成料堆,粉碎后的秸秆与发酵菌液A的质量比为1∶1~1.5;保持料堆的温度在25~35℃之间,持续发酵4~7天,得到已接种菌液A发酵了4~7天的秸秆;5)发酵菌液B的配制;6)接种菌液B发酵:将已接种菌液A发酵了4~7天的秸秆翻动并与发酵菌液B按质量比20~30∶1混匀;保持料堆温度在25~35℃之间,持续发酵5~8天后,整个发酵过程结束。该方法具有生产成本低、降解率高的特点。The invention relates to a method for processing crop stalks. A method for treating crop stalks, characterized in that it comprises the following steps: 1) preparation of a compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent; 2) crushing straw raw materials; 3) Preparation of fermentation liquid A; 4) Fermentation of inoculation liquid A: evenly spray the fermentation liquid A on the crushed stalks, and pile them into piles, and the mass ratio of the crushed straw to the fermentation liquid A is 1:1 ~1.5; keep the temperature of the stockpile between 25~35°C, continue to ferment for 4~7 days, and obtain the stalks that have been inoculated with bacterial solution A and fermented for 4~7 days; 5) preparation of fermented bacterial solution B; 6) inoculation Fermentation of Bacterial Liquid B: Stir the stalks that have been inoculated with Bacterial Liquid A and fermented for 4 to 7 days and mix them with the fermented Bacterial Liquid B at a mass ratio of 20 to 30:1; keep the stockpile temperature between 25 and 35°C, After 5-8 days of fermentation, the whole fermentation process ends. The method has the characteristics of low production cost and high degradation rate.
Description
技术领域 technical field
本发明涉及一种农作物秸秆的处理方法,具体涉及一种利用微生物高效降解农作物秸秆的方法。The invention relates to a method for processing crop straws, in particular to a method for efficiently degrading crop straws by using microorganisms.
背景技术 Background technique
我国是一个农业大国,据粗略统计,每年约产农作物秸秆7亿多吨,其中有89%尚未被利用或未被合理利用(如直接焚烧)。目前全世界被开发利用的农林纤维副产物不足2%,我国约有50%以上的农林废弃物在田间地头被白白烧掉。每年因农林废弃物焚烧不仅造成直接经济损失达数亿元,而且由于焚烧而产生的滚滚浓烟及排放的大量有害气体严重影响了公路、航空的安全,污染了环境,对气候、生态等也造成了严重的影响。同时随着全球经济的飞速发展,地球上石油、煤炭的储量正以惊人的速度减少,能源危机成了世界大多数国家所面临的一个严峻问题。另外由于对资源的破坏性开采和利用,人类赖以生存的环境正在不断恶化,对可再生资源利用的研究与开发的可持续发展战略已在世界各国逐步展开,植物纤维素资源的开发利用对解决粮食和资源短缺以及环境污染问题有极其深远的意义。my country is a large agricultural country. According to rough statistics, more than 700 million tons of crop straw are produced every year, 89% of which have not been utilized or rationally utilized (such as direct incineration). At present, less than 2% of agricultural and forestry fiber by-products are developed and utilized in the world, and more than 50% of agricultural and forestry wastes in my country are burned in vain in the fields. Every year, the incineration of agricultural and forestry waste not only causes direct economic losses of hundreds of millions of yuan, but also produces billowing smoke and a large amount of harmful gases due to incineration, which seriously affects the safety of roads and aviation, pollutes the environment, and affects climate and ecology. had a serious impact. At the same time, with the rapid development of the global economy, the reserves of oil and coal on the earth are decreasing at an alarming rate, and the energy crisis has become a serious problem faced by most countries in the world. In addition, due to the destructive exploitation and utilization of resources, the environment on which human beings depend is deteriorating. The sustainable development strategy for the research and development of renewable resource utilization has been gradually carried out in countries all over the world. The development and utilization of plant cellulose resources has a great impact on It has far-reaching significance to solve the problems of food and resource shortage and environmental pollution.
目前,秸秆的主要出路有秸秆还田;饲料化利用;秸秆气化、固化及炭化;作生产食用菌的培养基料;工业应用等。秸秆还田包括就地还田、快速沤肥和堆肥等。其中秸秆直接还田可增加土壤有机质含量、培肥土壤,对农业的可持续发展有重要意义,但还有很多问题尚未解决。首先,秸秆的C/N比高,不仅不利于土壤微生物降解,而且还可能导致作物缺氮而生长不良;其次,秸秆还可能传播部分病害,导致作物减产等。快速沤肥和堆肥则存在木质纤维素的降解问题。饲料化技术主要包括青贮氨化、发酵法以及生产单细胞蛋白。在我国可用作饲料的农作物秸秆有多种,但具有共同的营养特点:蛋白质、可溶性碳水化合物、矿物质和胡萝卜素含量低,而粗纤维含量高,因此消化率低,适口性差。要改善适口性,提高消化率,关键在于木质纤维素的降解。工业应用包括造纸,生物降解生产淀粉、酒精、醋酸等,易造成严重的环境污染,而且同样存在木质纤维素的降解问题。At present, the main outlets of straw include straw returning to the field; feed utilization; straw gasification, solidification and carbonization; as a culture material for the production of edible fungi; industrial applications, etc. Straw returning includes on-site returning, rapid retting and composting. Among them, returning straw directly to the field can increase the content of soil organic matter and fertilize the soil, which is of great significance to the sustainable development of agriculture, but there are still many problems that have not been resolved. First of all, the high C/N ratio of straw is not conducive to the degradation of soil microorganisms, but may also cause nitrogen deficiency and poor growth of crops; secondly, straw may also spread some diseases and cause crop yield reduction. Rapid retting and composting have the problem of degradation of lignocellulose. Feed technologies mainly include ammonification of silage, fermentation and production of single-cell protein. There are many kinds of crop straws that can be used as feed in our country, but they have common nutritional characteristics: low content of protein, soluble carbohydrates, minerals and carotene, and high content of crude fiber, so the digestibility is low and the palatability is poor. To improve palatability and increase digestibility, the key lies in the degradation of lignocellulose. Industrial applications include papermaking, biodegradable production of starch, alcohol, acetic acid, etc., which are likely to cause serious environmental pollution, and also have the problem of degradation of lignocellulose.
由于木质纤维素自身分子量大,木质素、半纤维素及纤维素相互缠结,结构复杂,性质稳定,所以目前普遍使用的物理、化学降解方法均不能有效降解,并带来成本和环境问题。生物方法具有环保无污染,成本低,使用条件温和等特点,在快速有效降解木质纤维素类物质方面有巨大潜力。发明专利(CN101948753A)公开了“一种新型降解农作物秸秆的复合菌剂”,复合菌剂用于秸秆降解时,需要于30℃左右发酵30天,木质素的降解率较低仅为27.8%,而且由于缺乏降解半纤维素的菌株,因此,该复合菌剂在降解秸秆时存在各菌株协同作用较少,发酵时间长,木质纤维素整体降解率低的问题。发明专利(CN101058792A)公开了“一种高效秸秆分解复合菌群”,菌群由包含真菌、细菌和放线菌等十四种菌种组合到一起,然而该发明所述的菌群中康宁木霉与菌群中的其它菌株如黄孢原毛平革菌、枯草芽袍杆菌和假丝酵母等菌有强烈的营养竞争和拮抗作用,简单的组合在一起会引起菌群效率下降,相互消耗,导致极大的浪费,增加生产成本。Due to the large molecular weight of lignocellulose, lignin, hemicellulose and cellulose are entangled with each other, the structure is complex, and the properties are stable. Therefore, the commonly used physical and chemical degradation methods cannot be effectively degraded, and bring cost and environmental problems. Biological methods are environmentally friendly, non-polluting, low-cost, and mild in use, and have great potential in rapidly and effectively degrading lignocellulosic substances. Invention patent (CN101948753A) discloses "a new type of composite bacterial agent for degrading crop straw". When the composite bacterial agent is used to degrade straw, it needs to be fermented at about 30°C for 30 days, and the degradation rate of lignin is as low as 27.8%. Moreover, due to the lack of strains that degrade hemicellulose, the composite microbial agent has the problems of less synergistic effect of each strain when degrading straw, long fermentation time, and low degradation rate of lignocellulose as a whole. Invention patent (CN101058792A) discloses "a high-efficiency straw decomposition composite flora". The flora is composed of fourteen strains including fungi, bacteria and actinomycetes. Mold and other strains in the flora, such as Phanerochaete chrysosporium, Bacillus subtilis and Candida, have strong nutritional competition and antagonism. Simple combination will cause the efficiency of the flora to decrease and consume each other. Cause great waste and increase production cost.
发明内容 Contents of the invention
本发明的目的在于提供一种处理农作物秸秆的方法,该方法生产成本低、降解率高。The object of the present invention is to provide a method for processing crop straws, which has low production cost and high degradation rate.
为实现上述目的,本发明所采用的技术方案是:一种处理农作物秸秆的方法,其特征在于它包括如下步骤:In order to achieve the above object, the technical solution adopted in the present invention is: a method for processing crop stalks, characterized in that it comprises the following steps:
1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成(两组单独分装的菌剂构成);1) Preparation of compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent (two groups of separately packed bacterial agents);
①按各菌种所占质量百分数为:黄孢原毛平革菌(Phaerochaete chrysosporium)28%~38%、变色栓菌(Trametes versicolor)5%~10%、平菇菌(Pleurorus ostreatus)6%~12%、克鲁斯酵母菌(Canadian krusei)7~14%、枯草芽胞杆菌(Bacillus Subtilis)6%~11%和地衣芽胞杆菌(Bacillus Licheniformis)15~25%,选取黄孢原毛平革菌(Phaerochaete chrysosporium)、变色栓菌(Trametes versicolor)、平菇菌(Pleurorus ostreatus)、克鲁斯酵母菌(Canadian krusei)、枯草芽胞杆菌(Bacillus Subtilis)和地衣芽胞杆菌(Bacillus Licheniformis),混合,得到A菌剂;①According to the mass percentage of each bacterial species: Phaerochaete chrysosporium 28%~38%, Trametes versicolor 5%~10%, Pleurorus otreatus 6%~ 12%, Canadian krusei 7-14%, Bacillus Subtilis 6%-11%, and Bacillus Licheniformis 15-25%, select Phanerochaete chrysosporium ( Phaerochaete chrysosporium), Trametes versicolor, Pleurorus otreatus, Canadian krusei, Bacillus Subtilis and Bacillus Licheniformis, mixed to obtain A Bacteria;
②按各菌种所占质量百分数为:绿色木霉(Trichoderma Viride)30%~40%和康氏木霉(Trichoderma koningii)60%~70%,选取绿色木霉(Trichoderma Viride)和康氏木霉(Trichoderma koningii),混合,得到B菌剂;②According to the mass percentages of various strains: Trichoderma Viride (Trichoderma Viride) 30% to 40% and Trichoderma koningii (Trichoderma koningii) 60% to 70%, select Trichoderma Viride (Trichoderma Viride) and Koningii Mildew (Trichoderma koningii), mixed, obtains B bacterial agent;
2)秸秆原料的粉碎:将农作物秸秆放入粉碎机中,粉碎成1cm~3cm的小段,得到粉碎后的秸秆;2) Crushing of straw raw materials: putting crop straws into a pulverizer, pulverizing them into small pieces of 1 cm to 3 cm, and obtaining pulverized straws;
3)发酵菌液A的配制:将A菌剂取出与水按质量比1∶100~200混合搅拌(使A菌剂均匀分散到水中),得到菌液;然后在菌液中加入碳酸氢铵,搅拌(使碳酸氢铵充分溶解),得到发酵菌液A;其中,碳酸氢铵的加入量为菌液质量的2~3%;3) Preparation of fermented bacterial liquid A: Take out the bacterial agent A and mix it with water at a mass ratio of 1:100-200 (so that the bacterial agent A is evenly dispersed in the water) to obtain the bacterial liquid; then add ammonium bicarbonate to the bacterial liquid , stirring (ammonium bicarbonate is fully dissolved) to obtain fermentation broth A; wherein, the addition of ammonium bicarbonate is 2 to 3% of the quality of the bacterial fluid;
4)接种菌液A发酵:将发酵菌液A均匀喷散在粉碎后的秸秆上,堆成料堆,粉碎后的秸秆与发酵菌液A的质量比为1∶1~1.5;保持料堆的温度在25~35℃之间(如果在冬天气温较低时,将料堆用薄膜覆盖),持续发酵4~7天,得到已接种菌液A发酵了4~7天的秸秆;4) Fermentation of the inoculated bacterial liquid A: Spray the fermented bacterial liquid A evenly on the crushed stalks and pile them into a stockpile. The mass ratio of the crushed straw to the fermented bacterial liquid A is 1:1 to 1.5; The temperature is between 25-35°C (if the temperature is low in winter, the stockpile is covered with a film), and the fermentation is continued for 4-7 days to obtain straw that has been inoculated with bacterial solution A and fermented for 4-7 days;
5)发酵菌液B的配制:将B菌剂取出与水按质量比1∶30~40混合搅拌(使B菌剂均匀分散到水中),得到发酵菌液B;5) Preparation of fermented bacterial liquid B: take out bacterial agent B and mix with water at a mass ratio of 1:30-40 (so that bacterial agent B is evenly dispersed in water) to obtain fermented bacterial liquid B;
6)接种菌液B发酵:将已接种菌液A发酵了4~7天的秸秆充分翻动并与发酵菌液B按质量比19~30∶1混匀;保持料堆温度在25~35℃之间,持续发酵5~8天后,整个发酵过程结束。6) Fermentation of inoculated bacterial solution B: Fully turn the straw that has been inoculated with bacterial solution A and fermented for 4 to 7 days and mix it with fermented bacterial solution B at a mass ratio of 19 to 30:1; keep the stockpile temperature at 25 to 35°C After continuous fermentation for 5-8 days, the whole fermentation process ends.
按上述方案,所述的农作物秸秆为油菜秸秆、棉花秸秆、芝麻秸秆、花生秸秆、稻草秸秆、麦秸秆、玉米秸秆、豆秸秆、高粱秸秆等秸秆中的任意一种或任意二种以上(含任意二种)的混合,任意二种以上(含任意二种)混合时为任意配比。According to the above scheme, the crop straw is any one or any two or more kinds (including Any two kinds of) mixing, any two or more kinds (including any two kinds) are mixed in any proportion.
本发明的有益效果是:The beneficial effects of the present invention are:
1、本发明中所涉及的菌株、药剂和设备在市场上方便易得,生产成本低。1. The bacterial strains, medicaments and equipment involved in the present invention are easily available in the market, and the production cost is low.
2、本发明所述的方法,操作简便,条件温合,容易控制,实现无难度。2. The method of the present invention has the advantages of simple and convenient operation, mild conditions, easy control and no difficulty in realization.
3、本发明所述之复配菌剂A菌剂中各菌株相互之间都进行过兼容性实验,不存在拮抗作用和明显营养竞争情况,因此,可相互协同,发酵时间短,降解效率高;B菌剂也同样如此。3. The strains in the compound microbial agent A bacterial agent of the present invention have been tested for compatibility with each other, and there is no antagonism and obvious nutritional competition. Therefore, they can cooperate with each other, the fermentation time is short, and the degradation efficiency is high. ; B bacteria agent is the same.
4、本发明所述之复配菌剂,包含有纤维素、半纤维素、木质素、蛋白质、和果胶等降解菌,品种丰富,搭配合理,可全面降解秸秆中各种有机物质。4. The compound bacterial agent of the present invention contains degrading bacteria such as cellulose, hemicellulose, lignin, protein, pectin, etc. It is rich in varieties and reasonable in combination, and can fully degrade various organic substances in straw.
5、本发明所述的方法,通过选接种A菌剂发酵一段时间后,再接种B菌剂,即可充分发挥各菌剂的功效,又可有效避免和缓解A与B菌剂之间存在的营养竞争作用,因此整体效率较高。5. In the method of the present invention, after inoculation of A bacterial agent and fermentation for a period of time, and then inoculation of B bacterial agent, the effects of each bacterial agent can be fully brought into play, and the presence of bacteria between A and B bacterial agents can be effectively avoided and alleviated. nutrient competition, so the overall efficiency is higher.
具体实施方式 Detailed ways
下面来描述本发明的优选方案;它包含在本发明保护的范围之内,但不限制本发明。The preferred version of the present invention is described below; it is included in the protection scope of the present invention, but does not limit the present invention.
实施例1:Example 1:
一种处理农作物秸秆的方法,它包括如下步骤:A method for processing crop stalks, comprising the steps of:
1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成(两组单独分装的菌剂构成);1) Preparation of compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent (two groups of separately packed bacterial agents);
①按各菌种所占质量百分数为:黄孢原毛平革菌(Phaerochaete chrysosporium)28%、变色栓菌(Trametes versicolor)10%、平菇菌(Pleurorus ostreatus)12%、克鲁斯酵母菌(Canadiankrusei)14%、枯草芽胞杆菌(Bacillus Subtilis)11%和地衣芽胞杆菌(Bacillus Licheniformis)25%,选取黄孢原毛平革菌(Phaerochaete chrysosporium)、变色栓菌(Trametes versicolor)、平菇菌(Pleurorus ostreatus)、克鲁斯酵母菌(Canadian krusei)、枯草芽胞杆菌(Bacillus Subtilis)和地衣芽胞杆菌(Bacillus Licheniformis),混合,得到A菌剂;①According to the mass percentage of each strain: Phaerochaete chrysosporium 28%, Trametes versicolor 10%, Pleurorus otreatus 12%, Krussia saccharomyces ( Canadiankrusei) 14%, Bacillus Subtilis 11% and Bacillus Licheniformis 25%, select Phaerochaete chrysosporium, Trametes versicolor, Pleurorus otreatus), Krusei saccharomyces (Canadian krusei), Bacillus subtilis (Bacillus Subtilis) and Bacillus licheniformis (Bacillus Licheniformis), mixed to obtain A microbial agent;
②按各菌种所占质量百分数为:绿色木霉(Trichoderma Viride)30%和康氏木霉(Trichoderma koningii)70%,选取绿色木霉(Trichoderma Viride)和康氏木霉(Trichodermakoningii),混合,得到B菌剂;2. account for the mass percent by each strain: Trichoderma Viride (Trichoderma Viride) 30% and Trichoderma koningii (Trichoderma koningii) 70%, choose Trichoderma Viride (Trichoderma Viride) and Kangshi Trichoderma (Trichoderma koningii), mix , to obtain B bacterial agent;
2)秸秆原料的粉碎:将100kg油菜秸秆放入粉碎机中,粉碎成2cm的小段,得到粉碎后的秸秆;2) Crushing of straw raw materials: put 100kg of rapeseed straw into a pulverizer, pulverize into small sections of 2 cm, and obtain pulverized straw;
3)发酵菌液A的配制:称取复配菌剂中的A菌剂0.5kg与100kg水混合搅拌(使A菌剂均匀分散到水中),得到菌液;然后在菌液中加入2.5kg碳酸氢铵(碳酸氢铵的加入量为菌液质量的2.49%);搅拌(使碳酸氢铵充分溶解),得到发酵菌液A;3) Preparation of fermented bacterial liquid A: Weigh 0.5kg of bacterial agent A in the compound bacterial agent and mix with 100kg of water (so that the bacterial agent A is evenly dispersed in water) to obtain the bacterial liquid; then add 2.5kg of bacterial liquid to the bacterial liquid Ammonium bicarbonate (the addition of ammonium bicarbonate is 2.49% of the quality of the bacterial liquid); stirring (to fully dissolve the ammonium bicarbonate) to obtain the fermentation liquid A;
4)接种菌液A发酵:将发酵菌液A均匀喷散在100kg粉碎后的秸秆上,堆成料堆;保持料堆的温度在30℃左右(如果在冬天气温较低时,将料堆用薄膜覆盖),持续发酵6天,得到已接种菌液A发酵了6天的秸秆;4) Fermentation of the inoculated bacterial liquid A: Spray the fermented bacterial liquid A evenly on 100kg of crushed stalks to form a pile; keep the temperature of the pile at about 30°C (if the temperature is low in winter, use the pile of film covering), continued fermentation for 6 days, and obtained stalks that had been inoculated with bacterial solution A and fermented for 6 days;
5)发酵菌液B的配制:取复配菌剂中的B菌剂0.25kg与10kg水混合搅拌(使B菌剂均匀分散到水中),得到发酵菌液B;5) Preparation of fermented bacterial liquid B: take 0.25 kg of B bacterial agent in the compound bacterial agent and mix and stir with 10 kg of water (so that B bacterial agent is evenly dispersed in water) to obtain fermented bacterial liquid B;
6)接种菌液B发酵:将已接种菌液A发酵了6天的秸秆充分翻动并与发酵菌液B按质量比20~30∶1混匀;保持料堆温度在30℃左右,持续发酵7天后,整个发酵过程结束。6) Fermentation of inoculated bacterial solution B: fully turn over the straw that has been inoculated with bacterial solution A and fermented for 6 days, and mix it with fermented bacterial solution B at a mass ratio of 20-30:1; keep the stockpile temperature at about 30°C, and continue to ferment After 7 days, the entire fermentation process is over.
测得发酵后的油菜秸秆中纤维素、半纤维素和木质素的降解率分别为46%、38%和42%,说明本发明降解率高。It is measured that the degradation rates of cellulose, hemicellulose and lignin in the fermented rape stalks are 46%, 38% and 42% respectively, which shows that the degradation rate of the present invention is high.
实施例2:Example 2:
一种处理农作物秸秆的方法,它包括如下步骤:A method for processing crop stalks, comprising the steps of:
1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成(两组单独分装的菌剂构成);1) Preparation of compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent (two groups of separately packed bacterial agents);
①按各菌种所占质量百分数为:黄孢原毛平革菌(Phaerochaete chrysosporium)38%、变色栓菌(Trametes versicolor)10%、平菇菌(Pleurorus ostreatus)12%、克鲁斯酵母菌(Canadiankrusei)14%、枯草芽胞杆菌(Bacillus Subtilis)11%和地衣芽胞杆菌(Bacillus Licheniformis)15%,选取黄孢原毛平革菌(Phaerochaete chrysosporium)、变色栓菌(Trametes versicolor)、平菇菌(Pleurorus ostreatus)、克鲁斯酵母菌(Canadian krusei)、枯草芽胞杆菌(Bacillus Subtilis)和地衣芽胞杆菌(Bacillus Licheniformis),混合,得到A菌剂;①According to the mass percentage of each strain: Phaerochaete chrysosporium 38%, Trametes versicolor 10%, Pleurorus otreatus 12%, Krussia saccharomyces ( Canadiankrusei) 14%, Bacillus Subtilis 11% and Bacillus Licheniformis 15%, select Phaerochaete chrysosporium, Trametes versicolor, Pleurorus otreatus), Krusei saccharomyces (Canadian krusei), Bacillus subtilis (Bacillus Subtilis) and Bacillus licheniformis (Bacillus Licheniformis), mixed to obtain A microbial agent;
②按各菌种所占质量百分数为:绿色木霉(Trichoderma Viride)40%和康氏木霉(Trichoderma koningii)60%,选取绿色木霉(Trichoderma Viride)和康氏木霉(Trichodermakoningii),混合,得到B菌剂;2. account for the mass percent by each strain: Trichoderma Viride (Trichoderma Viride) 40% and Trichoderma koningii (Trichoderma koningii) 60%, choose Trichoderma Viride (Trichoderma Viride) and Kangshi Trichoderma (Trichoderma koningii), mix , to obtain B bacterial agent;
2)秸秆原料的粉碎:将100kg稻草秸秆放入粉碎机中,粉碎成3cm的小段,得到粉碎后的秸秆;2) Crushing of straw raw materials: 100 kg of rice straw is put into a pulverizer, and pulverized into small sections of 3 cm to obtain pulverized straw;
3)发酵菌液A的配制:称取复配菌剂中的A菌剂0.6kg与120kg水混合搅拌(使A菌剂均匀分散到水中),得到菌液;然后在菌液中加入2.42kg的碳酸氢铵,搅拌(使碳酸氢铵充分溶解),得到发酵菌液A;3) Preparation of fermented bacterial liquid A: Weigh 0.6kg of bacterial agent A in the compound bacterial agent and mix and stir with 120kg of water (so that the bacterial agent A is evenly dispersed in water) to obtain the bacterial liquid; then add 2.42kg of bacterial liquid to the bacterial liquid ammonium bicarbonate, stirred (ammonium bicarbonate is fully dissolved), to obtain fermentation broth A;
4)接种菌液A发酵:将发酵菌液A均匀喷散在100kg粉碎后的秸秆上,堆成料堆;保持料堆的温度在28℃左右(如果在冬天气温较低时,将料堆用薄膜覆盖),持续发酵5天,得到已接种菌液A发酵了5天的秸秆;4) Fermentation of inoculated bacterial liquid A: Spray the fermented bacterial liquid A evenly on 100kg of crushed stalks to form a pile; keep the temperature of the pile at about 28°C (if the temperature is low in winter, use the pile of film covering), continued fermentation for 5 days, and obtained stalks that had been inoculated with bacterial solution A and fermented for 5 days;
5)发酵菌液B的配制:取复配菌剂中的B菌剂0.3kg与9kg水混合搅拌(使B菌剂均匀分散到水中),得到发酵菌液B;5) Preparation of fermentation bacterial liquid B: take 0.3 kg of B bacterial agent in the compound microbial agent and mix and stir with 9 kg of water (so that B bacterial agent is evenly dispersed into the water) to obtain fermentation bacterial liquid B;
6)接种菌液B发酵:将已接种菌液A发酵了5天的秸秆充分翻动并与发酵菌液B混匀;保持料堆温度在28℃左右,持续发酵6天后,整个发酵过程结束。6) Fermentation of inoculated bacterial solution B: Fully turn the straw that has been inoculated with bacterial solution A and fermented for 5 days and mix it with fermented bacterial solution B; keep the temperature of the stockpile at about 28°C, continue fermentation for 6 days, and the entire fermentation process ends.
测得发酵后的稻草秸秆中纤维素、半纤维素和木质素的降解率分别为45%、39%和43%,说明本发明降解率高。It is measured that the degradation rates of cellulose, hemicellulose and lignin in the fermented rice straw are 45%, 39% and 43% respectively, which shows that the degradation rate of the present invention is high.
实施例3:Example 3:
一种处理农作物秸秆的方法,它包括如下步骤:A method for processing crop stalks, comprising the steps of:
1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成(两组单独分装的菌剂构成);1) Preparation of compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent (two groups of separately packed bacterial agents);
①按各菌种所占质量百分数为:黄孢原毛平革菌(Phaerochaete chrysosporium)38%、变色栓菌(Trametes versicolor)5%、平菇菌(Pleurorus ostreatus)7%、克鲁斯酵母菌(Canadiankrusei)14%、枯草芽胞杆菌(Bacillus Subtilis)11%和地衣芽胞杆菌(Bacillus Licheniformis)25%,选取黄孢原毛平革菌(Phaerochaete chrysosporium)、变色栓菌(Trametes versicolor)、平菇菌(Pleurorus ostreatus)、克鲁斯酵母菌(Canadian krusei)、枯草芽胞杆菌(Bacillus Subtilis)和地衣芽胞杆菌(Bacillus Licheniformis),混合,得到A菌剂;①According to the mass percentage of each strain: Phaerochaete chrysosporium 38%, Trametes versicolor 5%, Pleurorus otreatus 7%, Krussia saccharomyces ( Canadiankrusei) 14%, Bacillus Subtilis 11% and Bacillus Licheniformis 25%, select Phaerochaete chrysosporium, Trametes versicolor, Pleurorus otreatus), Krusei saccharomyces (Canadian krusei), Bacillus subtilis (Bacillus Subtilis) and Bacillus licheniformis (Bacillus Licheniformis), mixed to obtain A microbial agent;
②按各菌种所占质量百分数为:绿色木霉(Trichoderma Viride)35%和康氏木霉(Trichoderma koningii)65%,选取绿色木霉(Trichoderma Viride)和康氏木霉(Trichodermakoningii),混合,得到B菌剂;2. account for the mass percent by each strain: Trichoderma Viride (Trichoderma Viride) 35% and Trichoderma koningii (Trichoderma koningii) 65%, choose Trichoderma Viride (Trichoderma Viride) and Kangshi Trichoderma (Trichoderma koningii), mix , to obtain B bacterial agent;
2)秸秆原料的粉碎:将100kg芝麻秸秆放入粉碎机中,粉碎成3cm的小段,得到粉碎后的秸秆;2) Crushing of straw raw materials: put 100kg of sesame straw into a pulverizer, pulverize into 3cm segments, and obtain pulverized straw;
3)发酵菌液A的配制:称取复配菌剂中的A菌剂0.6kg与100kg水混合搅拌(使A菌剂均匀分散到水中),得到菌液;然后在菌液中加入2.6kg的碳酸氢铵,搅拌(使碳酸氢铵充分溶解),得到发酵菌液A;3) Preparation of fermented bacterial liquid A: Weigh 0.6kg of bacterial agent A in the compound bacterial agent and mix and stir with 100kg of water (so that the bacterial agent A is evenly dispersed in water) to obtain the bacterial liquid; then add 2.6kg of bacterial liquid to the bacterial liquid ammonium bicarbonate, stirred (ammonium bicarbonate is fully dissolved), to obtain fermentation broth A;
4)接种菌液A发酵:将发酵菌液A均匀喷散在100kg粉碎后的秸秆上,堆成料堆;保持料堆的温度在30℃左右(如果在冬天气温较低时,将料堆用薄膜覆盖),持续发酵6天,得到已接种菌液A发酵了6天的秸秆;4) Fermentation of the inoculated bacterial liquid A: Spray the fermented bacterial liquid A evenly on 100kg of crushed stalks to form a pile; keep the temperature of the pile at about 30°C (if the temperature is low in winter, use the pile of film covering), continued fermentation for 6 days, and obtained stalks that had been inoculated with bacterial solution A and fermented for 6 days;
5)发酵菌液B的配制:取复配菌剂中的B菌剂0.3kg与10kg水混合搅拌(使B菌剂均匀分散到水中),得到发酵菌液B;5) Preparation of fermented bacterial liquid B: take 0.3 kg of B bacterial agent in the compound bacterial agent and mix and stir with 10 kg of water (so that B bacterial agent is evenly dispersed in water) to obtain fermented bacterial liquid B;
6)接种菌液B发酵:将已接种菌液A发酵了6天的秸秆充分翻动并与发酵菌液B混匀;保持料堆温度在30℃左右,持续发酵6天后,整个发酵过程结束。6) Fermentation of inoculated bacterial solution B: fully turn the straw that has been inoculated with bacterial solution A and fermented for 6 days and mix it with fermented bacterial solution B; keep the temperature of the stockpile at about 30°C, continue fermentation for 6 days, and the entire fermentation process ends.
测得发酵后的芝麻秸秆中纤维素、半纤维素和木质素的降解率分别为42%、37%和40%,说明本发明降解率高。The measured degradation rates of cellulose, hemicellulose and lignin in the fermented sesame stalks are 42%, 37% and 40% respectively, indicating that the degradation rate of the present invention is high.
实施例4:Example 4:
一种处理农作物秸秆的方法,其特征在于它包括如下步骤:A method for processing crop stalks, characterized in that it comprises the following steps:
1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成(两组单独分装的菌剂构成);1) Preparation of compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent (two groups of separately packed bacterial agents);
①按各菌种所占质量百分数为:黄孢原毛平革菌(Phaerochaete chrysosporium)38%、变色栓菌(Trametes versicolor)6%、平菇菌(Pleurorus ostreatus)6%、克鲁斯酵母菌(Canadiankrusei)14%、枯草芽胞杆菌(Bacillus Subtilis)11%和地衣芽胞杆菌(Bacillus Licheniformis)25%,选取黄孢原毛平革菌(Phaerochaete chrysosporium)、变色栓菌(Trametes versicolor)、平菇菌(Pleurorus ostreatus)、克鲁斯酵母菌(Canadian krusei)、枯草芽胞杆菌(Bacillus Subtilis)和地衣芽胞杆菌(Bacillus Licheniformis),混合,得到A菌剂;①According to the mass percentage of each bacterial species: Phaerochaete chrysosporium (Phaerochaete chrysosporium) 38%, Trametes versicolor (Trametes versicolor) 6%, Pleurorus otreatus (Pleurorus ostreatus) 6%, Krussia saccharomyces ( Canadiankrusei) 14%, Bacillus Subtilis 11% and Bacillus Licheniformis 25%, select Phaerochaete chrysosporium, Trametes versicolor, Pleurorus otreatus), Krusei saccharomyces (Canadian krusei), Bacillus subtilis (Bacillus Subtilis) and Bacillus licheniformis (Bacillus Licheniformis), mixed to obtain A microbial agent;
②按各菌种所占质量百分数为:绿色木霉(Trichoderma Viride)32%和康氏木霉(Trichoderma koningii)68%,选取绿色木霉(Trichoderma Viride)和康氏木霉(Trichodermakoningii),混合,得到B菌剂;2. account for the mass percent by each strain: Trichoderma Viride (Trichoderma Viride) 32% and Trichoderma koningii (Trichoderma koningii) 68%, choose Trichoderma Viride (Trichoderma Viride) and Kangshi Trichoderma (Trichoderma koningii), mix , to obtain B bacterial agent;
2)秸秆原料的粉碎:将100kg花生秸秆放入粉碎机中,粉碎成1cm的小段,得到粉碎后的秸秆;2) Crushing of straw raw materials: put 100kg of peanut straw into a pulverizer, pulverize into small pieces of 1 cm, and obtain pulverized straw;
3)发酵菌液A的配制:称取复配菌剂中的A菌剂0.6kg与120kg水混合搅拌(使A菌剂均匀分散到水中),得到菌液;然后在菌液中加入2.7kg的碳酸氢铵,搅拌(使碳酸氢铵充分溶解),得到发酵菌液A;3) Preparation of fermented bacterial liquid A: Weigh 0.6kg of bacterial agent A in the compound bacterial agent and mix and stir with 120kg of water (so that the bacterial agent A is evenly dispersed in water) to obtain the bacterial liquid; then add 2.7kg of bacterial liquid to the bacterial liquid ammonium bicarbonate, stirred (ammonium bicarbonate is fully dissolved), to obtain fermentation broth A;
4)接种菌液A发酵:将发酵菌液A均匀喷散在100kg粉碎后的秸秆上,堆成料堆;保持料堆的温度在25~35℃之间(如果在冬天气温较低时,将料堆用薄膜覆盖),持续发酵7天,得到已接种菌液A发酵了7天的秸秆;4) Fermentation of the inoculated bacterial liquid A: evenly spray the fermented bacterial liquid A on 100 kg of crushed stalks, and pile them into a stockpile; keep the temperature of the stockpile between 25 and 35°C (if the temperature is low in winter, put The stockpile is covered with a film), and the fermentation is continued for 7 days to obtain the stalk that has been inoculated with the bacterial solution A and fermented for 7 days;
5)发酵菌液B的配制:取复配菌剂中的B菌剂0.25kg与10kg水混合搅拌(使B菌剂均匀分散到水中),得到发酵菌液B;5) Preparation of fermented bacterial liquid B: take 0.25 kg of B bacterial agent in the compound bacterial agent and mix and stir with 10 kg of water (so that B bacterial agent is evenly dispersed in water) to obtain fermented bacterial liquid B;
6)接种菌液B发酵:将已接种菌液A发酵了7天的秸秆充分翻动并与发酵菌液B混匀;保持料堆温度在31℃左右,持续发酵8天后,整个发酵过程结束。6) Fermentation of inoculated bacterial solution B: Fully turn the straw that has been inoculated with bacterial solution A and fermented for 7 days and mix it with fermented bacterial solution B; keep the temperature of the stockpile at about 31°C, continue fermentation for 8 days, and the entire fermentation process ends.
测得发酵后的花生秸秆中纤维素、半纤维素和木质素的降解率分别为49%、41%和45%,说明本发明降解率高。It is measured that the degradation rates of cellulose, hemicellulose and lignin in the fermented peanut straw are 49%, 41% and 45% respectively, which shows that the degradation rate of the present invention is high.
实施例5:Example 5:
一种处理农作物秸秆的方法,它包括如下步骤:A method for processing crop stalks, comprising the steps of:
1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成(两组单独分装的菌剂构成);1) Preparation of compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent (two groups of separately packed bacterial agents);
①按各菌种所占质量百分数为:黄孢原毛平革菌(Phaerochaete chrysosporium)38%、变色栓菌(Trametes versicolor)10%、平菇菌(Pleurorus ostreatus)12%、克鲁斯酵母菌(Canadiankrusei)7%、枯草芽胞杆菌(Bacillus Subtilis)11%和地衣芽胞杆菌(Bacillus Licheniformis)22%,选取黄孢原毛平革菌(Phaerochaete chrysosporium)、变色栓菌(Trametes versicolor)、平菇菌(Pleurorus ostreatus)、克鲁斯酵母菌(Canadian krusei)、枯草芽胞杆菌(Bacillus Subtilis)和地衣芽胞杆菌(Bacillus Licheniformis),混合,得到A菌剂;①According to the mass percentage of each strain: Phaerochaete chrysosporium 38%, Trametes versicolor 10%, Pleurorus otreatus 12%, Krussia saccharomyces ( Canadiankrusei) 7%, Bacillus Subtilis 11% and Bacillus Licheniformis 22%, select Phaerochaete chrysosporium, Trametes versicolor, Pleurorus otreatus), Krusei saccharomyces (Canadian krusei), Bacillus subtilis (Bacillus Subtilis) and Bacillus licheniformis (Bacillus Licheniformis), mixed to obtain A microbial agent;
②按各菌种所占质量百分数为:绿色木霉(Trichoderma Viride)31%和康氏木霉(Trichoderma koningii)69%,选取绿色木霉(Trichoderma Viride)和康氏木霉(Trichodermakoningii),混合,得到B菌剂;2. account for the mass percent by each strain: Trichoderma Viride (Trichoderma Viride) 31% and Trichoderma Koningii (Trichoderma koningii) 69%, choose Trichoderma Viride (Trichoderma Viride) and Kangshi Trichoderma (Trichoderma Koningii), mix , to obtain B bacterial agent;
2)秸秆原料的粉碎:将农作物秸秆放入粉碎机中,粉碎成1cm的小段,得到粉碎后的秸秆;所述的农作物秸秆为棉花秸秆和麦秸秆,棉花秸秆和麦秸秆的质量各占50%;2) Crushing of straw raw materials: put crop straws into a pulverizer and crush them into 1cm segments to obtain crushed straws; the crop straws are cotton straw and wheat straw, and the quality of cotton straw and wheat straw is 50% each. %;
3)发酵菌液A的配制:将A菌剂取出与水按质量比1∶100混合搅拌(使A菌剂均匀分散到水中),得到菌液;然后在菌液中加入碳酸氢铵,搅拌(使碳酸氢铵充分溶解),得到发酵菌液A;其中,碳酸氢铵的加入量为菌液质量的2%;3) Preparation of fermentation broth A: Take out bacterial agent A and mix with water at a mass ratio of 1:100 (so that microbial agent A is evenly dispersed in water) to obtain bacterial liquid; then add ammonium bicarbonate to the bacterial liquid, stir (make ammonium bicarbonate fully dissolved), obtain fermented bacterium liquid A; Wherein, the add-on of ammonium bicarbonate is 2% of the bacterium liquid quality;
4)接种菌液A发酵:将发酵菌液A均匀喷散在粉碎后的秸秆上,堆成料堆,粉碎后的秸秆与发酵菌液A的质量比为1∶1;保持料堆的温度在25℃(如果在冬天气温较低时,将料堆用薄膜覆盖),持续发酵4天,得到已接种菌液A发酵了4天的秸秆;4) Fermentation of the inoculated bacterial liquid A: Spray the fermented bacterial liquid A evenly on the crushed stalks and pile them into a stockpile. The mass ratio of the crushed straw to the fermented bacterial liquid A is 1:1; keep the temperature of the stockpile at 25°C (if the temperature is low in winter, cover the stockpile with a film), continue fermentation for 4 days, and obtain stalks that have been inoculated with bacterial solution A and fermented for 4 days;
5)发酵菌液B的配制:将B菌剂取出与水按质量比1∶30混合搅拌(使B菌剂均匀分散到水中),得到发酵菌液B;5) Preparation of the fermentation liquid B: take out the bacterial agent B and mix it with water at a mass ratio of 1:30 (so that the bacterial agent B is evenly dispersed in the water) to obtain the fermentation liquid B;
6)接种菌液B发酵:将已接种菌液A发酵了4天的秸秆充分翻动并与发酵菌液B按质量比19∶1混匀;保持料堆温度在25℃,持续发酵5天后,整个发酵过程结束。6) Fermentation of inoculated bacterial solution B: fully turn the straw that has been inoculated with bacterial solution A and fermented for 4 days and mix it with fermented bacterial solution B at a mass ratio of 19:1; keep the stockpile temperature at 25°C, and continue to ferment for 5 days. The whole fermentation process is over.
测得发酵后的花生秸秆中纤维素、半纤维素和木质素的降解率分别为42%、39%和41%,说明本发明降解率高。The measured degradation rates of cellulose, hemicellulose and lignin in the fermented peanut stalks are 42%, 39% and 41% respectively, indicating that the degradation rate of the present invention is high.
实施例6:Embodiment 6:
一种处理农作物秸秆的方法,它包括如下步骤:A method for processing crop stalks, comprising the steps of:
1)复配菌剂的制备:该复配菌剂是由A菌剂和B菌剂组成(两组单独分装的菌剂构成);1) Preparation of compound bacterial agent: the compound bacterial agent is composed of A bacterial agent and B bacterial agent (two groups of separately packed bacterial agents);
①按各菌种所占质量百分数为:黄孢原毛平革菌(Phaerochaete chrysosporium)38%、变色栓菌(Trametes versicolor)10%、平菇菌(Pleurorus ostreatus)12%、克鲁斯酵母菌(Canadiankrusei)14%、枯草芽胞杆菌(Bacillus Subtilis)6%和地衣芽胞杆菌(Bacillus Licheniformis)20%,选取黄孢原毛平革菌(Phaerochaete chrysosporium)、变色栓菌(Trametes versicolor)、平菇菌(Pleurorus ostreatus)、克鲁斯酵母菌(Canadian krusei)、枯草芽胞杆菌(Bacillus Subtilis)和地衣芽胞杆菌(Bacillus Licheniformis),混合,得到A菌剂;①According to the mass percentage of each strain: Phaerochaete chrysosporium 38%, Trametes versicolor 10%, Pleurorus otreatus 12%, Krussia saccharomyces ( Canadiankrusei) 14%, Bacillus Subtilis 6% and Bacillus Licheniformis 20%, select Phaerochaete chrysosporium, Trametes versicolor, Pleurorus otreatus), Krusei saccharomyces (Canadian krusei), Bacillus subtilis (Bacillus Subtilis) and Bacillus licheniformis (Bacillus Licheniformis), mixed to obtain A microbial agent;
②按各菌种所占质量百分数为:绿色木霉(Trichoderma Viride)39%和康氏木霉(Trichoderma koningii)61%,选取绿色木霉(Trichoderma Viride)和康氏木霉(Trichodermakoningii),混合,得到B菌剂;2. account for the mass percent by each strain: Trichoderma Viride (Trichoderma Viride) 39% and Trichoderma Koningii (Trichoderma koningii) 61%, choose Trichoderma Viride (Trichoderma Viride) and Kangshi Trichoderma (Trichoderma Koningii), mix , to obtain B bacterial agent;
2)秸秆原料的粉碎:将农作物秸秆放入粉碎机中,粉碎成3cm的小段,得到粉碎后的秸秆;所述的农作物秸秆为豆秸秆和高粱秸秆,豆秸秆和高梁秸秆的质量各占50%;2) Crushing of straw raw materials: put crop straws into a pulverizer and crush them into small sections of 3 cm to obtain crushed straws; the crop straws are bean straw and sorghum straw, and the quality of bean straw and sorghum straw is 50% each. %;
3)发酵菌液A的配制:将A菌剂取出与水按质量比1∶200混合搅拌(使A菌剂均匀分散到水中),得到菌液;然后在菌液中加入碳酸氢铵,搅拌(使碳酸氢铵充分溶解),得到发酵菌液A;其中,碳酸氢铵的加入量为菌液质量的3%;3) Preparation of the fermentation liquid A: Take out the bacterial agent A and mix it with water at a mass ratio of 1:200 (so that the bacterial agent A is evenly dispersed in the water) to obtain the bacterial liquid; then add ammonium bicarbonate to the bacterial liquid, stir (make ammonium bicarbonate fully dissolved), obtain fermented bacterium liquid A; Wherein, the add-on of ammonium bicarbonate is 3% of the bacterium liquid quality;
4)接种菌液A发酵:将发酵菌液A均匀喷散在粉碎后的秸秆上,堆成料堆,粉碎后的秸秆与发酵菌液A的质量比为1∶1.5;保持料堆的温度在35℃之间(如果在冬天气温较低时,将料堆用薄膜覆盖),持续发酵7天,得到已接种菌液A发酵了7天的秸秆;4) Fermentation of inoculated bacterial liquid A: Spray the fermented bacterial liquid A evenly on the crushed stalks and pile them up into a stockpile. The mass ratio of the crushed straw to the fermented bacterial liquid A is 1:1.5; keep the temperature of the stockpile at Between 35°C (if the temperature is low in winter, cover the stockpile with a film), continue fermentation for 7 days, and obtain stalks that have been inoculated with bacterial solution A and fermented for 7 days;
5)发酵菌液B的配制:将B菌剂取出与水按质量比1∶40混合搅拌(使B菌剂均匀分散到水中),得到发酵菌液B;5) Preparation of the fermentation broth B: take out the bacterial agent B and mix it with water at a mass ratio of 1:40 (so that the bacterial agent B is evenly dispersed in the water) to obtain the fermentation broth B;
6)接种菌液B发酵:将已接种菌液A发酵了7天的秸秆充分翻动并与发酵菌液B按质量比30∶1混匀;保持料堆温度在35℃之间,持续发酵8天后,整个发酵过程结束。6) Fermentation of inoculated bacterial solution B: fully turn over the straw that has been inoculated with bacterial solution A and fermented for 7 days and mix it with fermented bacterial solution B at a mass ratio of 30:1; keep the stockpile temperature between 35°C and continue fermentation for 8 days Days later, the entire fermentation process is over.
测得发酵后的花生秸秆中纤维素、半纤维素和木质素的降解率分别为50%、43%和47%,说明本发明降解率高。It is measured that the degradation rates of cellulose, hemicellulose and lignin in the fermented peanut straw are 50%, 43% and 47% respectively, which shows that the degradation rate of the present invention is high.
本发明所列举的各原料,以及本发明各原料的上下限、区间取值,以及工艺参数(如温度、时间等)的上下限、区间取值都能实现本发明,在此不一一列举实施例。Each raw material enumerated in the present invention, and the upper and lower limits of each raw material of the present invention, the interval value, and the upper and lower limits of process parameters (such as temperature, time, etc.), the interval value can realize the present invention, do not enumerate one by one here Example.
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| CN110353087A (en) * | 2019-07-11 | 2019-10-22 | 南京农业大学 | The preparation method and fermented feed of rape stalk fermented feed |
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