CN102809542A - Determination method of polysaccharide content of rhodiola rosea injection - Google Patents
Determination method of polysaccharide content of rhodiola rosea injection Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 150000004676 glycans Chemical class 0.000 title claims abstract description 20
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 20
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 20
- 239000007924 injection Substances 0.000 title claims abstract description 8
- 238000002347 injection Methods 0.000 title claims abstract description 8
- 244000042430 Rhodiola rosea Species 0.000 title abstract 2
- 235000003713 Rhodiola rosea Nutrition 0.000 title abstract 2
- 239000000243 solution Substances 0.000 claims abstract description 61
- 238000012360 testing method Methods 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000013558 reference substance Substances 0.000 claims abstract description 15
- 238000005303 weighing Methods 0.000 claims abstract description 6
- 238000002835 absorbance Methods 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 14
- 238000009835 boiling Methods 0.000 claims description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 239000012085 test solution Substances 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 7
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 claims description 7
- 229910052708 sodium Inorganic materials 0.000 claims description 7
- 239000011734 sodium Substances 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical class [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000012937 correction Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 2
- 238000011084 recovery Methods 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 2
- 229940089206 anhydrous dextrose Drugs 0.000 abstract 2
- 238000003908 quality control method Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 239000000523 sample Substances 0.000 description 9
- 229940090044 injection Drugs 0.000 description 5
- YCCILVSKPBXVIP-UHFFFAOYSA-N 2-(4-hydroxyphenyl)ethanol Chemical compound OCCC1=CC=C(O)C=C1 YCCILVSKPBXVIP-UHFFFAOYSA-N 0.000 description 4
- 241001170115 Rhodiola kirilowii Species 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
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- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 2
- 206010002383 Angina Pectoris Diseases 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- DBLDQZASZZMNSL-QMMMGPOBSA-N L-tyrosinol Natural products OC[C@@H](N)CC1=CC=C(O)C=C1 DBLDQZASZZMNSL-QMMMGPOBSA-N 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
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- 235000004330 tyrosol Nutrition 0.000 description 2
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- 206010033557 Palpitations Diseases 0.000 description 1
- 241000590428 Panacea Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940093181 glucose injection Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000008354 sodium chloride injection Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000012430 stability testing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
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Abstract
The invention relates to a quality control method of a Chinese medicament, in particular to a determination method of polysaccharide content of rhodiola rosea injection. The determination method comprises the following steps of: (1) preparing a reference substance solution, i.e., precisely weighing 24.74 mg of D-anhydrous dextrose which is dried to constant weight at 105 DEG C, filling in a 25 ml measuring flask, adding water to dissolve and dilute to a graduation, and uniformly shaking to obtain the reference substance solution; (2) preparing a test object solution, i.e., precisely weighing 5 ml of the test object solution, filling in a 50 ml measuring flask, adding water to dissolve and dilute to a graduation and uniformly shaking, and precisely weighing 5 ml of the test object solution, filling in a 25 ml measuring flask, adding water to dilute to a graduation and uniformly shaking to obtain the test object solution; (3) preparing a standard curve; and (4) measuring the test object solution, wherein every 1 ml of test object solution contains greater than or equal to 6.0 mg of polysaccharide based on D-anhydrous dextrose (C6H10O6). The measuring method has the characteristics of accurate result and good reproducibility, recovery rate, sample recovery rate and stability.
Description
Technical field
The present invention relates to a kind of quality method of Chinese medicine preparation, specifically is gadol injection determination of polysaccharide method.
Background technology
In prior art, the rhodiola kirilowii Regel parenteral solution is the patented product that Tonghua Yusheng Medicine Co., Ltd. produces without competition, the accurate word Z20060361 of traditional Chinese medicines.Proterties: these article are faint yellow clear liquid to pale brown look.Composition: rhodiola kirilowii Regel.Adapt to symptom: promoting blood circulation and removing blood stasis.Coronary heart disease stable type fatigue property angina pectoris.Disease is seen: stabbing pain over the chest, and angina is fixed and is not moved, and pain is drawn the shoulder back of the body and arm inboard, and is uncomfortable in chest, palpitation and uneasiness, the words purple is dark, thready and hesitant pulse.Usage and dosage: drip-feed.One time 10ml adds in 250ml sodium chloride injection or 5% glucose injection, and once-a-day, 10 days is a course of treatment.
The preparation method: get rhodiola kirilowii Regel 1670g, boiling three times adds 6 times in water at every turn, decocts 2 hours, and collecting decoction filters; Filtrating is concentrated into the clear cream that relative density is 1.15～1.20 (50 ℃), adds ethanol and makes and contain alcohol amount and reach 70%, stirs, and refrigerate 24 hours (5～10 ℃), filtration; Deposition with a small amount of 70% washing with alcohol of 5～10 ℃ once filters, and merging filtrate reclaims ethanol and also is concentrated into the clear cream that relative density is 1.15～1.20 (50 ℃), adds ethanol again and makes and contain the alcohol amount and reach 85%; Stir, refrigerate 24 hours (5～10 ℃), filter, deposition with a small amount of 85% washing with alcohol of 5～10 ℃ once filters; Merging filtrate, adjust pH to 8.0 refrigerates 12 hours (5～10 ℃), filters, and filtrate recycling ethanol also is concentrated into the clear cream that relative density is 1.20～1.30 (50 ℃); Add water for injection 1000ml, refrigerate 12 hours (5～10 ℃), filter, filtrating adjust pH to 7.0, heated and boiled 30 minutes; Refrigerate 72 hours (5～10 ℃), filter, filtrating is 10000 ultrafiltration post ultrafiltration with molecular cut off, and ultrafiltrate concentrates, and freeze drying gets dry powder; Add the parenteral solution water and be mixed with 1000ml, filtering with microporous membrane, embedding, sterilization promptly gets.
Content Determination of Polysaccharide in the proper mass standard; After adopting the colour developing of sulfuric acid phenol; Measure with UV-VIS spectrophotometry, this method complex operation, time-consuming, and be difficult to obtain parallel result; And average recovery can only reach about 30%, so content Determination of Polysaccharide in the primary standard is revised.
Summary of the invention
The objective of the invention is to be directed against above-mentioned deficiency and a kind of gadol injection determination of polysaccharide method of checking effect that improves is provided.
Technical solution of the present invention is: gadol injection determination of polysaccharide method, and step is following:
(1) preparation of reference substance solution: precision takes by weighing the D-DEXTROSE ANHYDROUS 24.74mg that is dried to constant weight through 105 ℃, puts in the 25ml measuring bottle, is dissolved in water and is diluted to scale, shakes up, and promptly gets;
(2) preparation of need testing solution: precision is measured these article 5ml, puts in the 50ml measuring bottle, and thin up shakes up to scale, and precision is measured these article 5ml, puts in the 25ml measuring bottle, and thin up shakes up to scale, promptly gets.
(3) preparation of typical curve: precision is measured reference substance solution 0,0.3,0.4,0.5,0.6,0.7,0.8ml, puts respectively in the 25ml measuring bottle, and precision adds water to 2ml, accurate respectively adding 3; 5-dinitrosalicylic acid test solution 1.5ml, mixing, heating is 5 minutes in boiling water bath, takes out; Be cooled to room temperature immediately, thin up shakes up to scale, places 10 minutes; With first be blank, according to UV-VIS spectrophotometry, measure absorbance in the 488nm wavelength; With the absorbance is ordinate, and concentration is horizontal ordinate, the drawing standard curve;
(4) mensuration of need testing solution: precision is measured need testing solution 1ml, puts in the 25ml measuring bottle, adds 3mol/L hydrochloric acid solution 10ml, puts in the boiling water bath and heats 30 minutes; Take out, add 1 of instructions phenolphthalein solution after the cooling, transfer to saturated sodium hydroxide solution near when pink, dropping sodium solution 0.1mol/L again; Make pinkiness, put and be chilled to room temperature, add water to scale, shake up; Precision is measured this solution and each 1ml of correcting fluid, puts respectively in the 25ml measuring bottle, and precision adds water 1ml, and the method under the sighting target directrix curve item rises from " accurate respectively adding 3; 5-dinitrosalicylic acid test solution 1.5ml, mixing ", measures absorbance in accordance with the law, deducts blank correction; From the weight that typical curve is read D-DEXTROSE ANHYDROUS the test sample, calculate, promptly get; The every 1ml of these article contains polysaccharide with D-DEXTROSE ANHYDROUS (C
6H
10O
6) meter,>=6.0mg.
Advantage of the present invention is: 1, this method behaviour do easy, quick, highly sensitive, impurity disturb little, testing result accurately, reappearance, the recovery, average recovery, stability better.2, reducing substances and 3 such as pigment in this method, the method for measured value after the hydrolysis of hydrolysis before measurement setting examination and rectification is adopted in the colour developing of 5-dinitrosalicylic acid, simple and effective eliminating the interference of impurity such as pigment.
To combine embodiment that embodiment of the present invention is described in further detail below.
Description of drawings
Fig. 1 is the polysaccharide canonical plotting.
Fig. 2 is polysaccharide UV scanning figure in the test sample.
Fig. 3 is glucose reference substance UV scanning figure.
Embodiment
Gadol injection determination of polysaccharide method, its step is following:
1 method
1.1 instrument and reagent: UV-2550 ultraviolet-visible pectrophotometer; D-DEXTROSE ANHYDROUS reference substance is provided lot number 110833-200503 by Nat'l Pharmaceutical & Biological Products Control Institute.The sampling of Tonghua beautiful panacea industry company limited, lot number: 20080401,20080402,20080702,20090901, it is pure that reagent is analysis.
1.2 the preparation of reference substance solution: precision takes by weighing the about 24.74mg of D-DEXTROSE ANHYDROUS that is dried to constant weight through 105 ℃, puts in the 25ml measuring bottle, is dissolved in water and is diluted to scale, shakes up, and promptly gets (containing anhydrous grape 1mg among every 1ml).
1.3 the preparation of need testing solution: precision is measured these article 5ml, puts in the 50ml measuring bottle, and thin up shakes up to scale, and precision is measured these article 5ml, puts in the 25ml measuring bottle, and thin up shakes up to scale, promptly gets.
1.4 the preparation of correcting fluid: precision is measured need testing solution 1ml, puts in the 25ml measuring bottle, adds water 10ml, adds 1 of instructions phenolphthalein solution, drips dropping sodium solution (0.1mol/L) and makes pinkiness, and thin up shakes up to scale, promptly gets.
2 methodological studies
2.1 the preparation of typical curve: precision is measured reference substance solution 0,0.3,0.4,0.5,0.6,0.7,0.8ml, puts respectively in the 25ml measuring bottle, and precision adds water to 2ml, accurate respectively adding 3; 5-dinitrosalicylic acid test solution 1.5ml, mixing, heating is 5 minutes in boiling water bath, takes out; Be cooled to room temperature immediately, thin up shakes up to scale, places 10 minutes; With first be blank, according to UV-VIS spectrophotometry (an appendix V of Chinese Pharmacopoeia version in 2005 A), measure absorbance in the 488nm wavelength, be ordinate with the absorbance; Concentration is horizontal ordinate, the drawing standard curve, and the result sees table 1 (referring to Fig. 1).
Table 1 polysaccharide typical curve data
2.2 the mensuration of need testing solution: precision is measured need testing solution 1ml, puts in the 25ml measuring bottle, adds 3mol/L hydrochloric acid solution 10ml, puts in the boiling water bath and heats 30 minutes; Take out, add 1 of instructions phenolphthalein solution after the cooling, when transferring near pink with saturated sodium hydroxide solution, dropping sodium solution (0.1mol/L) makes pinkiness again; Put and be chilled to room temperature, add water to scale, shake up, precision is measured this solution and each 1ml of correcting fluid; Put respectively in the 25ml measuring bottle, precision adds water 1ml, and the method under the sighting target directrix curve item rises from " accurate respectively adding 3,5-dinitrosalicylic acid test solution 1.5ml; mixing ", measures absorbance in accordance with the law, deducts blank correction; From the weight that typical curve is read D-DEXTROSE ANHYDROUS the test sample, calculate, promptly get.
2.3 measure the selection of wavelength: get reference substance solution and need testing solution after the colour developing; In 200～600nm wavelength coverage, scan; Reference substance solution has absorption maximum in the 488nm wavelength as a result; Need testing solution has absorption maximum in the 486nm wavelength, so 488nm is for measuring wavelength (referring to Fig. 2,3).
2.4 the investigation in reaction time: get 3 parts of same need testing solutions (lot number 200800702); Operation in accordance with the law; The chromogenic reaction time was respectively 5,10,15 minutes; Measure absorbance in the 488nm wavelength and be respectively 0.612,0.614,0.610, see no significant difference, heated 5 minutes so the reaction time is decided to be in the boiling water bath from measuring the result.
2.5 the interference test of known composition rhodioside and tyrosol: get rhodioside reference substance solution (0.4848mg/ml) 2ml and tyrosol reference substance solution (0.218mg/ml) 2ml,, add 3mol/L hydrochloric acid solution 10ml, put in the boiling water bath and heated 30 minutes with putting in the 25ml measuring bottle; Take out, add 1 of instructions phenolphthalein solution after the cooling, when transferring near pink with saturated sodium hydroxide solution, dropping sodium solution (0.1mol/L) makes pinkiness again; Put and be chilled to room temperature, add water to scale, shake up, precision is measured 2ml; Put in the 25ml measuring bottle accurate adding 3,5-dinitrosalicylic acid test solution 1.5ml, mixing; Heating is 5 minutes in boiling water bath, takes out, and is cooled to room temperature immediately, and thin up is to scale; Shake up, measure absorbance in the 488nm wavelength immediately, its value is 0.212, and showing has interference to polysaccharide determination; Placing 5 minutes, is 0.025 measuring its absorbance, and placing and measuring its absorbance after 10 minutes is 0.004, almost noiseless to measuring; The sugar that produces after the rhodioside hydrolysis is described, though can be with 3, the reaction of 5-dinitrosalicylic acid test solution; But the compound that generates is unstable, thereby definite noiseless to polysaccharide determination after 10 minutes, measures so the regulation reacted solution was placed after 10 minutes.
2.6 replica test: the sample (lot number 20080702) of getting same lot number; 6 parts of independent as stated above mensuration; Polyoses content (mg/ml) is respectively as a result: 16.45,16.00,15.96,16.28,15.83,16.20, and average content is 16.12mg/ml, RSD is 1.41%.The result shows that repeatability better.
2.7 accuracy test: sample solution (lot number 20080702, the content 16.12mg/ml) 0.25ml that the preparation precision of need testing solution is measured known content,, put respectively in the 25ml measuring bottle totally by 9 parts; Accurate D-DEXTROSE ANHYDROUS reference substance solution (concentration 1.0316mg/ml) 2.5,3.0, the 3.5ml of adding, each 3 parts, thin up shakes up to scale; Precision is measured 1ml, puts in the 25ml measuring bottle, adds 3mol/L hydrochloric acid solution 10ml; Put in the boiling water bath and heated 30 minutes, take out, add 1 of instructions phenolphthalein solution after the cooling; When transferring near pink with saturated sodium hydroxide solution, dropping sodium solution (0.1mol/L) makes pinkiness again, puts and is chilled to room temperature; Add water to scale, shake up, promptly get.
The preparation of correcting fluid: precision is measured sample solution (lot number 20080702) 0.25ml, puts in the 25ml measuring bottle, and thin up is to scale; Shake up, precision is measured 1ml, puts in the 25ml measuring bottle; Add 1 of instructions phenolphthalein solution, dropping sodium solution (0.1mol/L) makes pinkiness, and thin up is to scale; Shake up, promptly get.
Determination method: precision is measured above-mentioned need testing solution and each 1ml of correcting fluid, puts respectively in the 25ml measuring bottle, and the method under the sighting target directrix curve item rises from " accurate respectively adding 3; 5-dinitrosalicylic acid test solution 1.5ml, mixing ", measures absorbance in accordance with the law; Deduct blank correction; From the weight that typical curve is read D-DEXTROSE ANHYDROUS the test sample, calculate, promptly get.The result sees table 2.
Table 2 polysaccharide accuracy test result
The result shows that average recovery is better.
2.8 stability test:
2.8.1 need testing solution in acid hydrolysis alkali with after the polyoses content that records when placing 24 hours of solution no change almost.
2.8.2 the need testing solution after the colour developing is measured absorbance 1 time at regular intervals, the result sees table 3.
Solution stability testing result after table 3 colour developing
| Time (minute) | 10 | 20 | 40 | 60 ? 90 120 ? RSD（%） |
| Absorbance | 0.643 | 0.642 | 0.643 | 0.643 ?0.642? 0.642? ?0.085 |
The result shows that the solution after the colour developing of these article is stable in 2 hours.
3 sample determination result and limits
Measure 4 lot sample article according to the text method, the result sees table 4.
Table 44 lot sample article assay results (mg/ml)
| Lot number | The content average content |
| 20080401 | 13.47 13.93 ? 13.7 |
| 20080402 | 14.60 14.78 ? 14.7 |
| 20080702 | 16.00 16.28 ?16.1 |
| 20090901 | 10.88 10.49 ? 10.7 |
According to the mensuration result to above 4 lot sample article, and the assay limit in the primary standard, the content limit of these article is decided to be: the every 1ml of these article contains polysaccharide with D-DEXTROSE ANHYDROUS (C
6H
10O
6) meter,>=6.0mg.
Claims (1)
1. gadol injection determination of polysaccharide method is characterized in that step is following:
(1) preparation of reference substance solution: precision takes by weighing the D-DEXTROSE ANHYDROUS 24.74mg that is dried to constant weight through 105 ℃, puts in the 25ml measuring bottle, is dissolved in water and is diluted to scale, shakes up, and promptly gets;
(2) preparation of need testing solution: precision is measured these article 5ml, puts in the 50ml measuring bottle, and thin up shakes up to scale, and precision is measured these article 5ml, puts in the 25ml measuring bottle, and thin up shakes up to scale, promptly gets;
(3) preparation of typical curve: precision is measured reference substance solution 0,0.3,0.4,0.5,0.6,0.7,0.8ml, puts respectively in the 25ml measuring bottle, and precision adds water to 2ml, accurate respectively adding 3; 5-dinitrosalicylic acid test solution 1.5ml, mixing, heating is 5 minutes in boiling water bath, takes out; Be cooled to room temperature immediately, thin up shakes up to scale, places 10 minutes; With first be blank, according to UV-VIS spectrophotometry, measure absorbance in the 488nm wavelength; With the absorbance is ordinate, and concentration is horizontal ordinate, the drawing standard curve;
(4) mensuration of need testing solution: precision is measured need testing solution 1ml, puts in the 25ml measuring bottle, adds 3mol/L hydrochloric acid solution 10ml, puts in the boiling water bath and heats 30 minutes; Take out, add 1 of instructions phenolphthalein solution after the cooling, transfer to saturated sodium hydroxide solution near when pink, dropping sodium solution 0.1mol/L again; Make pinkiness, put and be chilled to room temperature, add water to scale, shake up; Precision is measured this solution and each 1ml of correcting fluid, puts respectively in the 25ml measuring bottle, and precision adds water 1ml, and the method under the sighting target directrix curve item rises from " accurate respectively adding 3; 5-dinitrosalicylic acid test solution 1.5ml, mixing ", measures absorbance in accordance with the law, deducts blank correction; From the weight that typical curve is read D-DEXTROSE ANHYDROUS the test sample, calculate, promptly get; The every 1ml of these article contains polysaccharide with D-DEXTROSE ANHYDROUS (C
6H
10O
6) meter,>=6.0mg.
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