CN103074202B - In vitro array photo-stimulation system - Google Patents

Abstract

An in vitro array photostimulation system, used to provide photostimulation to a petri dish for experimental research on photosensitive cells, including a control system, a drive circuit electrically connected to the control system, and a printed circuit board connected to the drive circuit Electrically connected LED arrays, the control system sends control electrical signals to the drive circuit to drive the LED arrays to group and work at regular intervals; the LED arrays are opposite to the culture dish, and the LED arrays include at least two An LED lamp group of LED lamps, the LED lamps are evenly distributed. The control system sends control electrical signals to the drive circuit to drive the LED arrays composed of evenly distributed LED lamps to work at regular intervals, and the evenly distributed LED lamp arrays realize uniform illumination of light intensity.

Description

In vitro array photostimulation system

【Technical field】

The invention relates to the field of optoelectronic technology, in particular to an in vitro array light stimulation system.

【Background technique】

The "Optogenetic Technology System" technology (Optogenetic Technology) is a brand-new biotechnology that was first developed in the United States and Germany in the last three years and integrates genetic engineering, electrophysiology, optics and electronic engineering. This technology is based on the method of gene therapy, using a virus vector to introduce a gene (ChR2) that can encode a light-sensitive cation channel protein cloned from the green alga Chlamydomonas reinhardtii into a specific cell subpopulation for expression. When stimulated by blue light (472nm), Na ⁺ ions will enter the cell, causing depolarization of the cell membrane and exciting the cell. In addition, there is another light-regulated chloride ion pump protein gene NpHR extracted from green algae, which is sensitive to yellow light (593nm wavelength). After it is cloned into a certain cell and expressed, the chloride ion Channel proteins allow ^Cl- to enter the cell to repolarize the cell membrane, thereby returning the cell to quiescence, so that cell activity can be controlled at the level of specific cell subpopulations using alternating blue or yellow light. This technology has cell-specificity in space and millisecond-level precision in time, so it has great application potential in both scientific research and clinical drug development.

At present, there is no readily available device for experimental research on light stimulation, which greatly limits the application of the "optogenetic technology system" in the study of cell function in vitro. At present, there are high-power LEDs on the market that can be used for light stabbing research, but there are the following problems: the light intensity of a single LED is not enough, or it is difficult to meet the requirements with low-heat LEDs, and high-power LEDs generate a lot of heat and are difficult to dissipate heat. , the light intensity is difficult to evenly irradiate and other problems.

【Content of invention】

Based on this, it is necessary to provide an in vitro array photostimulation system capable of achieving average light intensity irradiation.

An in vitro array photostimulation system, which is used to provide photostimulation to a petri dish for photosensitive cell experimental research, is characterized in that it includes a control system, a drive circuit electrically connected to the control system, and a printed circuit board with The drive circuit is electrically connected to the LED array, and the control system sends control electrical signals to the drive circuit to drive the LED array into groups and work regularly; the LED array is opposite to the culture dish, and the LED array includes at least Two LED lamp groups with several LED lamps evenly distributed.

Preferably, it further includes a heat dissipation device electrically connected to the driving circuit, and the heat dissipation device is fixed on the other side of the printed circuit board opposite to the LED array.

Preferably, the heat dissipation device includes heat dissipation fins arranged in a matrix and a fan located on one side of the heat dissipation fins, and the heat dissipation fins are arranged in a matrix at intervals.

Preferably, the LED lamp group includes a first LED lamp group and a second LED lamp group; the second LED lamp group surrounds the first LED lamp group.

Preferably, the LED lamps in the first LED lamp group are distributed in a regular triangle, and the LED lamps in the second LED lamp group are distributed in a regular hexagon; the regular hexagon coincides with the center of the regular triangle.

Preferably, the first LED lamp group includes three LED lamps arranged at three vertices of a regular triangle, and the second LED lamp group includes six LED lamps arranged at six vertices of a regular hexagon.

Preferably, the LED array includes LED lamps emitting light of different wavelengths, such as 470 nm and 590 nm; the LED lamps emitting light of the same wavelength are connected in series.

Preferably, the control system includes an induction unit, a lower computer unit, an upper computer unit, a display unit, and a power supply unit, wherein: the induction unit communicates with the lower computer unit to detect parameter information of the culture dish, and send the parameter information to the lower computer unit; the lower computer unit communicates with the upper computer unit, the sensing unit and the display unit, and sends a control electric signal to the drive circuit according to the parameter information to drive the The LED array works; the lower computer unit also sends the parameter information to the upper computer unit and the display unit; the upper computer unit receives and displays the parameter information and changes the control parameters of the lower computer unit; The power supply unit is electrically connected to the lower computer unit and supplies power to the lower computer unit; the display unit receives and displays the parameter information.

Preferably, both the upper computer unit and the lower computer unit include a wireless module, and the upper computer unit and the lower computer unit communicate through the wireless module.

Preferably, the parameter information includes light intensity information, temperature information, pH value information and light duration information.

The above-mentioned in vitro array light stimulation system sends control electrical signals to the drive circuit through the control system to drive the LED arrays composed of evenly distributed LED lamps to work in groups and at regular intervals, and the evenly distributed LED lamp arrays realize uniform illumination of light intensity.

【Description of drawings】

Fig. 1 is a structural schematic diagram of an in vitro array photostimulation system in an embodiment;

Fig. 2 is a schematic structural view of an LED array in an embodiment;

Fig. 3 is a schematic structural view of an LED array in an embodiment;

FIG. 4 is a schematic diagram of a heat dissipation device in an embodiment.

【Detailed ways】

In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

As shown in Figure 1, an in vitro array light stimulation system is used to provide light stimulation to a culture dish for experimental research on light-sensitive cells. The external array photostimulation system includes a control system 200, a drive circuit 300, an LED array 400, a printed circuit board 500 (as shown in FIG. 4), a cooling device 600 and supporting feet 700 (as shown in FIG. 4).

The control system 200 is electrically connected to the driving circuit 300, and the driving circuit 300 is electrically connected to the LED array 400 and the cooling device 600. The control system 200 sends control electrical signals to the driving circuit 300 to drive the LED array 400 to group and work regularly, and at the same time sends control electrical signals. The signal is sent to the driving circuit 300 to drive the cooling device 600 to work.

As shown in FIG. 2 , the LED array includes three LED arrays 400 arranged uniformly. In other embodiments, the LED array can be provided with multiple LED arrays 400 according to the needs of the current situation.

The LED array 400 includes LED lamps emitting light of different wavelengths, and the emitted light of the LED lamps includes blue light with a wavelength of 470 nanometers and yellow light with a wavelength of 590 nanometers. LED lamps that emit the same wavelength of light are connected in series as a group. In this embodiment, every three LED lamps emitting the same wavelength are connected in series as a group.

The LED array 400 is set to emit LED lamps of different wavelengths to meet the requirements of different wavelengths of light for experimental research on light-sensitive cells. Under the control of the control system 200, LED lamps emitting the same wavelength of light are connected in series to form a group, which can realize grouping and timing control. Multiple LED lamps in the LED array 400 can achieve the required light intensity through the superposition of light spots. In other embodiments, according to different light-sensitive cell experimental studies on different light wavelength requirements for light stimulation, the in vitro array light stimulation system can be equipped with LEDs that emit different light wavelengths, and are not limited to two wavelengths of 470 nanometers and 590 nanometers. kind. In addition, according to different experimental studies of light-sensitive cells, LED lights emitting the same wavelength of light can be connected in parallel, or LED lights emitting different wavelengths of light can be connected in series.

As shown in FIG. 3 , the LED array 400 includes at least two LED lamp groups 420 with several LED lamps evenly distributed. The uniform distribution of LED lights can provide light-intensity average irradiation for experimental research on light-sensitive cells. In one embodiment, the LED light group 420 includes a first LED light group 422 and a second LED light group 424 , and the second LED light group 424 surrounds the first LED light group 422 . Under the condition that the average light intensity can be provided, it is not limited to two groups of LED lamp groups 420 provided in the LED array 400. In other embodiments, it can be one group, three groups, four groups or even More LED light sets 420. The arrangement and grouping of the LED lamp groups 420 here is not contradictory to the above-mentioned series connection of the same LED lamps. In other words, LED lamps emitting the same wavelength of light in different LED lamp groups 420 can be connected in series through an electrical connection.

In one embodiment, the LED lamps in the first LED lamp group 422 are distributed in a regular triangle, and the LED lamps in the second LED lamp group 424 are distributed in a regular hexagon, and the regular hexagon coincides with the center of the regular triangle. The first LED lamp group 422 includes three LED lamps arranged at three vertices of a regular triangle, and the second LED lamp group 424 includes six LED lamps arranged at six vertices of a regular hexagon. Referring to Fig. 2, the LED array 400 is composed of nine LED lamps, and the central connection line of the six peripheral LED lamps forms a regular hexagon, and the six peripheral LED lamps respectively fall on the six vertices of the regular hexagon; The central connection line of the three LDE lamps forms an equilateral triangle, and the three LED lamps inside fall on the three vertices of the equilateral triangle respectively. In other embodiments, the LED lamps in the LED lamp group 420 may be distributed in other shapes, such as rectangles, circles, rhombuses, etc., under the condition of providing uniform illumination with light intensity.

As shown in FIG. 4 , an LED array composed of several LED arrays 400 is provided on one side of the printed circuit board 500 , and a heat sink 600 is provided on the other side of the LED array 400 opposite to the LED array. When the in vitro array light stimulation system is used to provide light stimulation for experimental research on light-sensitive cells, the LED array composed of the LED array 400 is disposed under the printed circuit board 500 and faces the culture dish relative to the heat sink 600 . In a preferred embodiment, the printed circuit board 500 is made of an aluminum-based material, so that the in vitro array light stimulation system can be firmly arranged on the printed circuit board 500, and the aluminum-based material is conducive to the work of the LED array composed of the LED array 400. The generated heat energy is conducted to the cooling device 600, so that the heat can be dissipated efficiently, and the heat dissipation performance of the in vitro array photostimulation system can be maintained to meet the requirements of research work.

The heat sink 600 includes a heat sink 620 and a fan 640 . In one embodiment, on the aluminum-based printed circuit board, the number and position of LED arrays on the other side opposite to the side provided with the cooling device 600 correspond to the number and position of the LED arrays. It is oppositely arranged on one side of the three heat sinks 620 . In other embodiments, the required number of cooling fins 620 and fans 640 is set according to the requirements of experimental research on light-sensitive cells or corresponding to the number and position of LED arrays.

In a preferred embodiment, the cooling fins 620 are arranged in a matrix, and gaps are left in the rows and columns of the matrix arrangement. The gaps that are relatively wide are defined as columns, and the gaps that are relatively narrow are defined as rows. Arranging the cooling fins 620 in a matrix can increase the contact area between the cooling fins 620 and the circulating air, so that heat can be dissipated faster.

In a preferred embodiment, the fan 640 is located on one side of the heat sink 620 , and the columns of the heat sink 620 arranged in a matrix are opposite to the fans 640 . The fan 640 can blow to the upper and lower sides of the aluminum-based printed circuit board 500 at the same time, which enhances the effect of heat dissipation. At the same time, the fan 640 can also blow to the lower computer unit 220 in the control system 200 and the circuit layer composed of the drive circuit 300 (not shown in the figure). shown) to dissipate heat from the circuit layer. Since the gaps left by the arrangement of the columns of the cooling fins 620 are wider than the rows, it is beneficial to the circulation of the air, and it is beneficial for the fan 640 to blow off the heat on the cooling fins 620 .

In a preferred embodiment, the supporting feet 700 are fixed on the printed circuit board 500 for supporting the printed circuit board 500 and the devices on it can be erected above the culture dish. When the light-sensitive cell experiment of the external array light stimulation system is carried out, the LED array 400 on the external array light stimulation system can be irradiated on the culture dish at a good angle.

As shown in FIG. 1 , the control system 200 includes a sensing unit 210 , a lower computer unit 220 , an upper computer unit 230 , a display unit 240 and a power supply unit 250 .

The sensing unit 210 communicates with the lower computer unit 220 for detecting parameter information of the culture dish and sending the parameter information to the lower computer unit 220 . The sensing unit 210 includes a photosensitive sensor, a temperature sensitive sensor, and a pH value sensor, so the detected parameter information of the petri dish includes light intensity information, temperature information, pH value information, and light duration information.

The lower computer unit 220 is located above the heat sink 600 and is disposed in the circuit layer. The lower computer unit 220 communicates with the sensing unit 210 , the upper computer unit 230 and the display unit 240 . The lower computer unit 220 sends control electrical signals to the driving circuit 300 to drive the LED array 400 to work according to the parameter information, and sends the parameter information to the upper computer unit 230 and the display unit 240 . The lower computer unit 220 sends control electrical signals to the driving circuit 300 to drive the LED array 400 to realize the light intensity, flashing frequency, duty cycle and group control of the LED lights. In this embodiment, the lower computer unit 220 includes a single chip microcomputer, a wireless transmitting module and a wireless receiving module.

Both the upper computer unit 230 and the lower computer unit 220 include a wireless module 225 , and the upper computer unit 230 and the lower computer unit 220 communicate through the wireless module 225 . The upper computer unit 230 receives and displays parameter information, and changes the control parameters of the lower computer unit 230 . In this embodiment, the upper computer unit 230 is a computer terminal, and the computer terminal receives the uploaded by the lower computer unit 220 and displays and records parameter information including light intensity, temperature, pH value, and illumination duration, etc. The operating state of the system. In other embodiments, the upper computer unit 230 and the lower computer unit 220 can use serial communication.

The power supply unit 240 is a battery pack, which is electrically connected to the lower computer unit 220 and supplies power to the lower computer unit 220 . It can be understood that when the upper computer unit 230 and the lower computer unit 220 communicate through the serial port, the power supply unit 240 can be removed, and the power required by the lower computer unit 220 can be provided by the upper computer unit 230 through the serial port.

The display unit 250 receives and displays parameter information including light intensity, temperature, pH value and light duration.

The driving circuit 300 is disposed on the circuit layer, and the circuit layer is disposed above the heat sink. In one embodiment, the driving circuit 300 has several identical groups, including the NUD4001 chip and its peripheral circuit driving, and the signal is sent by the control system 200 .

The above-mentioned embodiments only express several implementation modes of the present invention, and the description thereof is relatively specific and detailed, but should not be construed as limiting the patent scope of the present invention. It should be pointed out that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

Claims (8)

1. an external array light stimulus system, be used to the culture dish of photaesthesia cell experiment research that light stimulus is provided, it is characterized in that, comprise Controlling System, the driving circuit being electrically connected to described Controlling System and be located at the LED battle array being electrically connected to described driving circuit on printed circuit board (PCB), also comprise the heat abstractor being electrically connected to driving circuit, described heat abstractor is fixed on the another side that described printed circuit board (PCB) is relative with being provided with described LED array, described printed circuit board (PCB) is made by aluminium base material, described Controlling System sends control electrical signal and drives described LED battle array grouping to described driving circuit, timing working, described LED battle array is relative with described culture dish, and described LED battle array comprises at least two LED lamp groups that have several LED lamps, and described LED lamp is uniformly distributed,

Described Controlling System comprises sensing unit, lower computer unit, upper computer unit, display unit and power supply unit, wherein:

Described sensing unit and described lower computer unit communications, for detection of the parameter information of described culture dish, and send to described lower computer unit by described parameter information; Sensing unit comprises photosensor, temp-sensitive sensor, pH value sensor;

Described lower computer unit and described upper computer unit, sensing unit and display unit communication, according to described parameter information send control electrical signal to described driving circuit to drive the work of described LED battle array; Described lower computer unit also sends described parameter information to described upper computer unit and display unit;

The control parameter that described upper computer unit receives and shows described parameter information and change described lower computer unit;

Described power supply unit is electrically connected to described lower computer unit and is the power supply of described lower computer unit;

Described display unit receives and shows described parameter information.

2. external array light stimulus system according to claim 1, is characterized in that, described heat abstractor comprises into the matrix radiator element of arranging and the fan that is positioned at a side of described radiator element, and described radiator element becomes matrix to be intervally arranged.

3. external array light stimulus system according to claim 1, is characterized in that, described LED lamp group comprises a LED lamp group and the 2nd LED lamp group; Described the 2nd LED lamp group is surrounded a described LED lamp group.

4. external array light stimulus system according to claim 3, is characterized in that, the LED lamp in a described LED lamp group is equilateral triangle and distributes, and the LED lamp in described the 2nd LED lamp group is regular hexagon and distributes; Described regular hexagon and described equilateral triangle center superposition.

5. external array light stimulus system according to claim 4, is characterized in that, a described LED lamp group comprises three LED lamp of being located at three summits of equilateral triangle, and described the 2nd LED lamp group comprises six LED lamp of being located at six summits of regular hexagon.

6. the external array light stimulus system described in claim 1 or 5, is characterized in that, described LED battle array comprises the LED lamp of launching different wavelengths of light, and described wavelength comprises 470 nanometers, 590 nanometers; The identical described LED lamp series connection of optical wavelength of transmitting.

7. the external array light stimulus system of stating according to claim 1, is characterized in that, described upper computer unit and lower computer unit include wireless module, and described upper computer unit and lower computer unit are by described wireless module communication.

8. the external array light stimulus system that claim 7 is stated, is characterized in that, described parameter information comprises intensity signal, temperature information, pH value information and light irradiation time information.