CN103204949A - Extraction method of low-acyl non-clear type gellan gum - Google Patents
Extraction method of low-acyl non-clear type gellan gum Download PDFInfo
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- CN103204949A CN103204949A CN2013101346892A CN201310134689A CN103204949A CN 103204949 A CN103204949 A CN 103204949A CN 2013101346892 A CN2013101346892 A CN 2013101346892A CN 201310134689 A CN201310134689 A CN 201310134689A CN 103204949 A CN103204949 A CN 103204949A
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- gellan gum
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- 229920002148 Gellan gum Polymers 0.000 title claims abstract description 50
- 235000010492 gellan gum Nutrition 0.000 title claims abstract description 49
- 239000000216 gellan gum Substances 0.000 title claims abstract description 49
- 238000000605 extraction Methods 0.000 title abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 48
- 125000002252 acyl group Chemical group 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000000926 separation method Methods 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 13
- 238000005406 washing Methods 0.000 claims abstract description 9
- 229910052783 alkali metal Inorganic materials 0.000 claims abstract description 7
- -1 alkali metal salt Chemical class 0.000 claims abstract description 7
- 239000002585 base Substances 0.000 claims description 12
- 238000010792 warming Methods 0.000 claims description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 5
- 239000000693 micelle Substances 0.000 claims description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 4
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- 208000006558 Dental Calculus Diseases 0.000 claims description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 2
- 230000002378 acidificating effect Effects 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 claims description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 2
- 235000011152 sodium sulphate Nutrition 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 238000001914 filtration Methods 0.000 abstract description 3
- 238000004090 dissolution Methods 0.000 abstract 3
- 230000001105 regulatory effect Effects 0.000 abstract 2
- 230000003113 alkalizing effect Effects 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 239000008187 granular material Substances 0.000 abstract 1
- 238000007781 pre-processing Methods 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005453 pelletization Methods 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000001117 sulphuric acid Substances 0.000 description 2
- 235000011149 sulphuric acid Nutrition 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 241000736110 Sphingomonas paucimobilis Species 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008291 lyophilic colloid Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003408 sphingolipids Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Jellies, Jams, And Syrups (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses an extraction method of low-acyl non-clear type gellan gum, and the extraction method comprises the following steps of: (1) raising the temperature of fermentation liquor to 70-100 DEG C, alkalizing, and regulating the pH value to 9.0-11.5; (2) keeping for 5-20 minutes, removing acyl to obtain a gellan gum dissolution solution with the acyl removed; (3) adding alkali metal salt which accounts for 0.5-2.0 percent of the weight of the gellan gum dissolution solution to the gellan gum dissolution solution, regulating the pH value to subacidity to form gel, and carrying out solid-liquid separation to obtain a nontransparent gellan gum sheet; (4) cutting the nontransparent gellan gum sheet into gum granules, washing by using lower alcohol, and carrying out solid-liquid separation again to obtain gellan gum powder; and (5) drying and smashing to obtain a low-acyl non-clear type gellan gum finished product. The extraction method disclosed by the invention directly carries out acyl removing treatment on the fermentation liquor by modifying the preprocessing process of the low-acyl gellan gum and omits the filtering and defecating process, thereby decreasing the processing link, reducing the production cost of the gellan gum and ensuring the excellent solubility property of the low-acyl gellan gum product.
Description
Technical field
The present invention relates to the microbial fermentation field, particularly relate to the extracting method of the non-clean-type gellan gum of a kind of low acyl group.
Background technology
Gelling gum (Gellan gum) be the U.S. triumphant can (KELCO) company a kind of lyophilic colloid of exploitation, it is to be zymophyte with moving sphingolipid Zymomonas mobilis (Sphingomonas paucimobilis) less, through several grades of fermentation culture, fermented liquid is produced by a series of later stage extraction processes again.
Gelling gum can be described as one of the most excellent thickening material of present performance, stablizer, and has good gel property.The gelling gum gel have be easy to use, have good local flavor release property, higher thermostability, in mouth, easily melt, time of gel and temperature adjustable, gel are not subject to the pH influence, various advantages such as matter structure are stablized, had to product.
At present, gelling gum mainly contains two kinds of low-acyl clean-type gellan gum and the non-clean-type gellan gums of low acyl group.The low-acyl clean-type gellan gum product has been removed most of insoluble substance owing to adopt filtration process in the production process, and therefore the gel clear that forms has very high transparence.And contain partial protein (mass percentage content is about 17%) and other impurity in the low non-clean-type gellan gum product of acyl group, therefore the gel that forms is opaque.At present; in fields such as foodstuffs industry; the application of low-acyl clean-type gellan gum is more common; and the application of the low non-clean-type gellan gum product of acyl group does not obtain due understanding, so each gelling gum manufacturer does not all develop the concrete production technique that is suitable for the low non-clean-type gellan gum of acyl group at present.In fact; the low non-clean-type gellan gum of acyl group has nearly all advantage of low-acyl clean-type gellan gum; such as; can substitute carrageenin in the meat product Application Areas; in baking series products filling material, bring into play splendid thermal stability and water conservation in the gel-like pet food; strengthen product special flavour release property etc., therefore be particularly suitable for being used in the product that transparency is not required.
Summary of the invention
The technical problem to be solved in the present invention provides the extracting method of the non-clean-type gellan gum of a kind of low acyl group, and its operating process is simple, and cost is low, is fit to large-scale industrial production.
For solving the problems of the technologies described above, the extracting method of the non-clean-type gellan gum of low acyl group of the present invention comprises following processing step:
1) gellan gum fermentation liquid is warming up to 70～100 ℃, in fermented liquid, adds alkali, regulate pH value to 9.0～11.5;
2) fermented liquid that step 1) is obtained was kept 5～20 minutes under the temperature of step 1), pH condition, obtained removing the gelling gum sol solutions of acyl group;
3) to step 2) add an alkali metal salt that accounts for sol solutions quality 0.5～2.0% in the gelling gum sol solutions that obtains, regulate the pH value to slightly acidic, solid-liquid separation behind the gel obtains opaque gelling gum film;
4) the gelling gum film that step 3) is obtained is cut into micelle, and with the lower alcohol washing, solid-liquid separation obtains the gelling gum powder;
5) the gelling gum powder for drying that step 4) is obtained is also pulverized, and obtains the low non-clean-type gellan gum finished product of acyl group.
In the described step 1), the temperature of gellan gum fermentation liquid is preferable between 80～95 ℃, and the pH value is preferable between 9.5～10.5.
Described step 2) in, generally keeps 8～15 minutes deacylated tRNA bases.
In the described step 3), an alkali metal salt can use one or more the combination in Repone K, sodium-chlor, vitriolate of tartar, the sodium sulfate, and the consumption of an alkali metal salt is the 0.8～1.2% preferable of gelling gum sol solutions quality; Regulate the pH value and can use one or more combination in hydrochloric acid, acetic acid, citric acid, xitix, the lactic acid, generally the pH value is adjusted to 4.0～5.5 preferable.
In the described step 4), lower alcohol can use one or more the combination in ethanol, n-propyl alcohol, Virahol, propyl carbinol, the isopropylcarbinol, and the consumption of described lower alcohol is good with 0.3～0.6 times of gellan gum fermentation liquid original volume.
In the described step 5), drying temperature is generally 90～120 ℃, and be 1～2 hour time of drying.
Compare with the conventional production process of low-acyl gellan gum, the extracting method of the non-clean-type gellan gum of low acyl group of the present invention has the following advantages and beneficial effect:
1. the direct deacylated tRNA base of fermented liquid is handled, and without filtering clarification process, has therefore reduced processing link, has significantly reduced the production cost of gelling gum;
2. can not introduce divalent-metal ion in the production process, guarantee the solubility property of low-acyl gellan gum product fine;
3. in the end link is used lower alcohol washing gelling gum material, easy handling not only, and reduced the consumption of lower alcohol to greatest extent, further reduced production cost.
Embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
It is as follows that present embodiment extracts the concrete processing step of non-clear type low-acyl gellan gum:
(1) intensification of fermented liquid and pH regulator: gellan gum fermentation liquid directly is warming up to 85 ℃, adds mass percent concentration in the fermented liquid and be 10% potassium hydroxide solution, regulate pH value to 10.0;
(2) the deacylated tRNA base of fermented liquid is handled: the fermented liquid that step (1) is obtained was kept 15 minutes under 85 ℃, pH value 10.0 conditions, removed fully to guarantee the gelling gum acyl group, obtained the gelling gum sol solutions behind the deacylated tRNA base;
(3) gel of sol solutions and solid-liquid separation: add the sodium-chlor that accounts for sol solutions quality 1.2% in the gelling gum sol solutions behind step (2) the gained deacylated tRNA base, and the adding concentration of volume percent is 10% hydrochloric acid soln, regulate pH value to 4.5, use the chamber-type press filter solid-liquid separation behind the gel, obtain opaque low-acyl gellan gum film;
(4) pelletizing of gelling gum film, washing and solid-liquid separation: the film that step (3) is obtained is cut into to the micelle of 1cm * 1cm size, add fermented liquid original volume 0.3 times of amount, concentration of volume percent then and be 90% washed with isopropyl alcohol, after the whizzer solid-liquid separation, break up and obtain fluffy powder;
(5) drying of gelling gum powder, pulverizing: with the gelling gum powder that step (4) obtains, by boiling drying equipment, drying is 1.5 hours under 100 ℃, namely obtains non-clear type low-acyl gellan gum finished product after the pulverizing.
Embodiment 2
It is as follows that present embodiment extracts the concrete processing step in side of non-clear type low-acyl gellan gum:
(1) intensification of fermented liquid and pH regulator: gellan gum fermentation liquid directly is warming up to 90 ℃, adds mass percent concentration in the fermented liquid and be 10% sodium hydroxide solution, regulate pH value to 10.5;
(2) the deacylated tRNA base of fermented liquid is handled: the fermented liquid that step (1) is obtained was kept 8 minutes under 90 ℃, pH value 10.5 conditions, removed fully to guarantee the gelling gum acyl group, obtained the gelling gum sol solutions behind the deacylated tRNA base;
(3) gel of sol solutions and solid-liquid separation: add the Repone K that accounts for sol solutions quality 1.0% in the gelling gum sol solutions behind step (2) the gained deacylated tRNA base, and the adding concentration of volume percent is 10% sulphuric acid soln, regulate pH value to 5.0, use the chamber-type press filter solid-liquid separation behind the gel, obtain opaque low-acyl gellan gum film;
(4) pelletizing of gelling gum film, washing and solid-liquid separation: the film that step (3) is obtained is cut into the micelle of 1.5cm * 1.5cm size, add fermented liquid original volume 0.4 times of amount, concentration of volume percent then and be 90% washing with alcohol, after the whizzer solid-liquid separation, break up and obtain fluffy powder;
(5) drying of gelling gum powder, pulverizing: with the gelling gum powder that step (4) obtains, by vacuum dryer, drying is 1.5 hours under 90 ℃, namely obtains non-clear type low-acyl gellan gum finished product after the pulverizing.
Embodiment 3
It is as follows that present embodiment extracts the concrete processing step of non-clear type low-acyl gellan gum:
(1) intensification of fermented liquid and pH regulator: gellan gum fermentation liquid directly is warming up to 95 ℃, adds mass percent concentration in the fermented liquid and be 10% potassium hydroxide solution, regulate pH value to 10.5;
(2) the deacylated tRNA base of fermented liquid is handled: the fermented liquid that step (1) is obtained was kept 8 minutes under pH value 10.5 conditions at 80 ℃, removed fully to guarantee the gelling gum acyl group, obtained the gelling gum sol solutions behind the deacylated tRNA base;
(3) gel of sol solutions and solid-liquid separation: add the Repone K that accounts for sol solutions quality 1.2% in the gelling gum sol solutions behind step (2) the gained deacylated tRNA base, and the adding concentration of volume percent is 10% sulphuric acid soln, regulate pH value to 5.5, use the chamber-type press filter solid-liquid separation behind the gel, obtain opaque low-acyl gellan gum film;
(4) pelletizing of gelling gum film, washing and solid-liquid separation: the film that step (3) is obtained is cut into the micelle of 1cm * 1.5cm size, add fermented liquid original volume 0.5 times of amount, concentration of volume percent then and be 90% washing with alcohol, after the screw press solid-liquid separation, break up and obtain fluffy powder;
(5) drying of gelling gum powder, pulverizing: with the gelling gum powder that step (4) obtains, by vacuum dryer, drying is 2.0 hours under 90 ℃, namely obtains non-clear type low-acyl gellan gum finished product after the pulverizing.
Claims (10)
1. the extracting method of the low non-clean-type gellan gum of acyl group is characterized in that, comprises following processing step:
1) gellan gum fermentation liquid is warming up to 70～100 ℃, in fermented liquid, adds alkali, regulate pH value to 9.0～11.5;
2) fermented liquid that step 1) is obtained was kept 5～20 minutes under the temperature of step 1), pH condition, obtained removing the gelling gum sol solutions of acyl group;
3) to step 2) add an alkali metal salt that accounts for sol solutions quality 0.5～2.0% in the gelling gum sol solutions that obtains, regulate the pH value to slightly acidic, solid-liquid separation behind the gel obtains opaque gelling gum film;
4) the gelling gum film that step 3) is obtained is cut into micelle, and with the lower alcohol washing, solid-liquid separation obtains the gelling gum powder;
5) the gelling gum powder for drying that step 4) is obtained is also pulverized, and obtains the low non-clean-type gellan gum finished product of acyl group.
2. method according to claim 1 is characterized in that, step 1) is warming up to 80～95 ℃ with gellan gum fermentation liquid.
3. method according to claim 1 is characterized in that, step 1) is adjusted to 9.5～10.5 with the pH value.
4. method according to claim 1 is characterized in that step 2), the deacylated tRNA base time is 8～15 minutes.
5. method according to claim 1 is characterized in that, step 3), and the consumption of described an alkali metal salt is 0.8～1.2% of described gelling gum sol solutions quality.
6. method according to claim 1 or 5 is characterized in that, described an alkali metal salt comprises one or more the combination in Repone K, sodium-chlor, vitriolate of tartar, the sodium sulfate.
7. method according to claim 1 is characterized in that, step 3), the scope of described pH value are 4.0～5.5.
8. method according to claim 1 is characterized in that, step 4), and described lower alcohol comprises one or more the combination in ethanol, n-propyl alcohol, Virahol, propyl carbinol, the isopropylcarbinol.
9. according to claim 1 or 8 described methods, it is characterized in that the consumption of described lower alcohol is 0.3～0.6 times of gellan gum fermentation liquid original volume.
10. method according to claim 1 is characterized in that, step 5), and drying temperature is 90～120 ℃, be 1～2 hour time of drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101346892A CN103204949A (en) | 2013-04-18 | 2013-04-18 | Extraction method of low-acyl non-clear type gellan gum |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013101346892A CN103204949A (en) | 2013-04-18 | 2013-04-18 | Extraction method of low-acyl non-clear type gellan gum |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862354A (en) * | 2014-02-24 | 2015-08-26 | 杨凤英 | Production method of low-acyl gellan gum used for oil and gas exploitation |
| CN106350554A (en) * | 2016-08-30 | 2017-01-25 | 新疆阜丰生物科技有限公司 | Extraction method of non-transparent type low-acyl gellan gum |
| EP3385390A1 (en) | 2017-04-07 | 2018-10-10 | DSM IP Assets B.V. | A method for preparing high transparent low acyl gellan gum |
| CN116135886A (en) * | 2023-04-18 | 2023-05-19 | 广州市乾相生物科技有限公司 | Extraction method of transparent low-acyl gellan gum |
| CN116655821A (en) * | 2023-06-13 | 2023-08-29 | 嘉保生物科技(保山)有限公司 | Post-extraction method of low-acyl transparent gellan gum |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862354A (en) * | 2014-02-24 | 2015-08-26 | 杨凤英 | Production method of low-acyl gellan gum used for oil and gas exploitation |
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| EP3385390A1 (en) | 2017-04-07 | 2018-10-10 | DSM IP Assets B.V. | A method for preparing high transparent low acyl gellan gum |
| CN116135886A (en) * | 2023-04-18 | 2023-05-19 | 广州市乾相生物科技有限公司 | Extraction method of transparent low-acyl gellan gum |
| CN116655821A (en) * | 2023-06-13 | 2023-08-29 | 嘉保生物科技(保山)有限公司 | Post-extraction method of low-acyl transparent gellan gum |
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Application publication date: 20130717 |