CN103210897A - Method of utilizing liquid detergent to trap and kill mycetophilidae in bemisia tabaci population - Google Patents

Abstract

The invention relates to a method for trapping and killing mosquitoes in whitefly populations by using detergent. The steps are as follows: (1) After mixing detergent and water at a volume ratio of 1: (99-199), the detergent is prepared diluent; (2) adding sucrose to the detergent diluent prepared in step (1) at a ratio of 2% to 10% by mass to obtain the bacteria and mosquito trap; (3) making the The fungus mosquito trap is added into a wide-mouthed container, and then placed in the population cage for raising Bemisia tabaci, and the fungus mosquito trap is replaced every 4 to 7 days. The method of the invention can not only trap and kill bacterial mosquitoes but also trap and kill a small amount of thrips, but has no effect on the growth and reproduction of whitefly.

Description

A method of trapping and killing bacterial mosquitoes in whitefly populations by using detergent

technical field

The invention relates to a method for trapping and killing bacterial mosquitoes in whitefly populations by using detergent, and belongs to the field of agricultural biotechnology.

Background technique

The whitefly Bemisia tabaci (Gennadius) is a worldwide agricultural pest. Due to its wide host spectrum, strong explosiveness and great harm, it is listed as one of the 100 most harmful invasive species in the world by the International Union for Conservation of Nature (IUCN). At the same time, it is also the only insect that has been dubbed the title of "super pest" in the history of the international scientific and technological circles. In recent years, with the spread of the geminivirus Tomato Yellow Leaf Curl Virus (TYLCV) transmitted by Bemisia tabaci, the harm of Bemisia tabaci has become more and more serious, and even led to the extinction of tomato production in some areas such as Jiangsu, Henan, and Shandong. Therefore, studying the invasion mechanism and control methods of Bemisia tabaci is a hot issue in the world today. In order to prevent and control Bemisia tabaci, it is first necessary to raise the population of Bemisia tabaci. The breeding of Bemisia tabaci is carried out with cotton in northern areas. In cotton planting, common The main pests are spider mites, cotton bollworms, thrips, etc. During the planting of cotton seedlings in the laboratory, fungus mosquitoes also occur from time to time. The fungus mosquitoes belong to the class Insecta, Diptera, Diptera, Lycoriella fanestralis, Lycoriella fanestralis. The appearance of A. acupunctatus directly affected the cultivation of cotton seedlings and hindered the reproduction of whitefly populations.

The main hazards of Acupunctatus are edible and medicinal fungi such as oyster mushrooms, shiitake mushrooms, and white fungus. In recent years, it has been found that the hazards of Acupoints also occur on orchids and some flowers. The hosts of Acrimony mosquitoes are extensive, and their feeding habits are miscellaneous. Its harm to plants mainly has the following aspects. One is that the larvae eat the roots of cotton seedlings and promote the death of cotton seedlings. The second is that the fungal mosquito is mainly saprophytic, which accelerates the decomposition of organic matter in the pot; the third is that the adult insects fly around in the population cage of Bemisia tabaci and spread cotton viruses. Seriously affect the growth of cotton.

The main reasons for the large number of A. tabaci in the population cages where Bemisia tabaci are raised are as follows: First, because the room temperature for cultivating Bemisia tabaci and cotton seedlings in the greenhouse is about 27°C, and the sunshine time is set to 12L: 12D; Mycetoma acridida has many generations in one year, and there is no overwintering phenomenon in the southern region; the conditions in the laboratory are conducive to the large-scale reproduction of A. Secondly, in order to ensure the reproduction of Bemisia tabaci, the laboratory will take measures to prevent the entry of foreign insects, which not only reduces the natural enemies of Bemisia tabaci, but also reduces the natural enemies of A. In addition, the eggs of A. acupunctatus are laid on the surface or crevices of the compost, which makes the control of A. acupoints very difficult. The most important point is that there are physical control and chemical control for the prevention and control of the sharp-eyed fungus mosquito. Phosphorous EC or malathion, deltafluthrin EC, permethrin EC, chlorpyrifos-methyl and other treatment media, but this will inevitably come into contact with the leaves, or be absorbed by cotton seedlings, causing bemisia tabaci Death, so neither chemical control nor physical control is suitable in the population of whitefly. In general, because the temperature and humidity are suitable for the growth of A. tabaci, the lack of natural enemies and the inability to effectively control the A. tabaci make the A. tabaci populations multiply in large numbers.

Contents of the invention

The invention aims at the deficiencies of the prior art, and provides a method for trapping and killing the bacterial mosquitoes in the Bemisia tabaci population by using detergent.

A method for trapping and killing bacterial mosquitoes in whitefly populations by using detergent, the steps are as follows:

(1) After mixing detergent and water in a volume ratio of 1: (99-199), the detergent dilution is prepared;

(2) Adding sucrose to the detergent diluent prepared in step (1) at a ratio of 2% to 10% by mass to obtain a mosquito trap;

(3) Add the mosquito trap prepared in step (2) into a wide-mouthed container, then place it in a population cage for raising Bemisia tabaci, and replace the mosquito trap every 4 to 7 days.

Preferably according to the present invention, in the step (1), the detergent and water are mixed in a ratio of 1:99 by volume.

Preferably according to the present invention, the mass percentage of sucrose added to the detergent dilution in the step (2) is 6%.

Preferably according to the present invention, the volume of the wide-mouth container described in the step (3) is 80-120ml, and the diameter of the opening is 5-8cm;

More preferably, the volume of the wide-mouth container in the step (3) is 100ml, and the diameter of the opening is 6.5cm.

Preferably according to the present invention, in the step (3), the amount of the bacterial and mosquito trap is 40-60% of the volume of the wide-mouthed container.

More preferably, in the step (3), the amount of the bacterial and mosquito trap is 50% of the volume of the wide-mouthed container.

Preferably according to the present invention, 4 wide-mouthed containers containing bacteria and mosquito traps are placed in the population cage in the step (3).

The above reagents were all commercially available products unless otherwise specified.

Beneficial effect

1. The method of the present invention can effectively trap and kill bacteria mosquitoes, and the control effect is remarkable;

2. The method of the present invention uses dishwashing liquid and sucrose to prepare the fungus and mosquito trap, the method is simple, and the implementation cost is low;

3. The method of the present invention can not only trap and kill bacterial mosquitoes but also trap and kill a small amount of thrips, but it does not affect the growth and reproduction of whitefly.

Detailed ways

The technical solutions of the present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

The detergent described in Example 1 is Liby detergent, the detergent described in Example 2 is Diaopai detergent, and the detergent described in Example 3 is White Cat detergent; sell products.

Example 1

A method for trapping and killing bacterial mosquitoes in whitefly populations by using detergent, the steps are as follows:

(1) After mixing detergent and water at a volume ratio of 1:199, a diluted detergent liquid is obtained;

(2) adding sucrose to the liquid detergent diluent obtained in step (1) at a ratio of 2% by mass ratio to obtain a mosquito trap;

(3) adding the antibacterial mosquito trap prepared in step (2) into a container with a volume of 80ml and an opening diameter of 8cm, the amount of the mosquito trap is 60% of the volume of the wide-mouth container, and then Place them in four cage corners in the population cages for raising Bemisia tabaci respectively, and replace the bacterial mosquito traps every 4 days.

Example 2

A method for trapping and killing bacterial mosquitoes in whitefly populations by using detergent, the steps are as follows:

(1) After mixing detergent and water at a volume ratio of 1:150, a diluted detergent liquid is prepared;

(2) adding sucrose to the liquid detergent diluent prepared in step (1) at a ratio of 10% by mass to obtain a fungus and mosquito trap;

(3) adding the antibacterial mosquito trap prepared in step (2) into a container with a volume of 120ml and an opening diameter of 5cm, the amount of the antibacterial mosquito trap is 40% of the volume of the wide-mouth container, and then Place them in four cage corners in the population cages for raising Bemisia tabaci respectively, and replace the fungus mosquito traps every 7 days.

Example 3

A method for trapping and killing bacterial mosquitoes in whitefly populations by using detergent, the steps are as follows:

(1) After mixing detergent and water at a volume ratio of 1:99, a diluted detergent liquid is obtained;

(2) adding sucrose to the liquid detergent diluent prepared in step (1) at a ratio of 6% by mass to obtain a mosquito trap;

(3) adding the antibacterial mosquito trap prepared in step (2) into a container with a volume of 100ml and an opening with a diameter of 6.5cm, the amount of the antibacterial mosquito trap is 50% of the volume of the wide-mouth container, Then place respectively at four cage corners in the population cage of raising Bemisia tabaci, and change the bacterium mosquito trap every 6 days.

Comparative example 1

(1) After mixing detergent and water at a volume ratio of 1:99, a diluted detergent liquid is obtained;

(2) Add the detergent diluent prepared in step (1) into a container with a volume of 120ml and an opening with a diameter of 5cm and a wide mouth. The amount of detergent diluent added is 40% of the volume of the wide-mouth container , and then placed at the four cage corners of the population cage that raised Bemisia tabaci, and the detergent dilution was changed every 6 days.

Comparative example 2

Adding water into a container with a volume of 100ml and an opening with a diameter of 6.5 cm, the amount of water added is 50% of the volume of the container with a wide mouth, and then placed in the four cage corners of the population cage for raising Bemisia tabaci place, replace the bacteria and mosquito traps every 6 days.

Comparative example 3

(1) Prepare a sucrose solution at a ratio of 6% by mass;

(2) Add the sucrose solution prepared in step (1) into a container with a volume of 100ml and an opening with a diameter of 6.5cm. The amount of the sucrose solution added is 50% of the volume of the container with a wide mouth. The sucrose solution was replaced every 6 days at the corners of the four cages in the population cage of Bemisia tabaci.

Result analysis

As can be seen from the above table, the total number of mosquitoes that can be trapped and killed in Example 1 reaches 152, and 22 heads of thrips are trapped. Trapped and killed 430 mosquitoes and 174 thrips. And in comparative example 2 and comparative example 3, the number of trapping and killing bacteria mosquitoes is 2 and 10 respectively, and the quantity of trapping and killing thrips is all 0, so it can be seen that this comparative example 2 and comparative example 3 are almost equal to bacteria mosquitoes and thrips. There is no trapping and killing effect, and the number of trapping and killing bacteria mosquitoes and thrips is respectively 75 and 10 for the trapping agent of the 100-fold diluted detergent semen in comparative example 1, indicating that a single detergent dilution is effective for bacteria mosquitoes and thrips. Thrips trapping has a certain effect, but not significant. The numbers of whiteflies trapped and killed in the examples and the comparative examples are all relatively close. Such a conclusion can be drawn from the Examples and Comparative Examples. Example 3 has a good trapping and killing effect on bacterial mosquitoes, and can trap and kill some thrips, but has no obvious effect on the growth of Bemisia tabaci.

Claims (8)

1. method of utilizing bacterium mosquito in the liquid detergent trapping Bemisia tabaci population is characterized in that step is as follows:

(1) with liquid detergent and water 1:(99～199 by volume) mix after, make the liquid detergent dilution;

(2) sucrose of 2%～10% ratio adding by mass percentage in the liquid detergent dilution that step (1) makes makes bacterium mosquito attractant;

(3) the bacterium mosquito attractant that step (2) is made adds in the container of wide mouth, places the population cage of raising Bemisia tabaci then, bacterium mosquito attractant of replacing in per 4～7 days.

2. the method for claim 1 is characterized in that, in the described step (1) liquid detergent and water by volume the ratio of 1:99 mix.

3. the method for claim 1 is characterized in that, the mass percent that adds sucrose in the described step (2) in the liquid detergent dilution is 6%.

4. the method for claim 1 is characterized in that, the vessel volume of wide mouthful described in the described step (3) is 80～120ml, and the diameter of opening is 5～8cm.

5. method as claimed in claim 4 is characterized in that, wide mouthful vessel volume is 100ml in the described step (3), and the diameter of opening is 6.5cm.

6. the method for claim 1 is characterized in that, in the described step (3) addition of bacterium mosquito attractant be wide mouthful vessel volume 40～60%.

7. method as claimed in claim 6 is characterized in that, in the described step (3) addition of bacterium mosquito attractant be wide mouthful vessel volume 50%.

8. the method for claim 1 is characterized in that, places 4 wide mouthful of containers that bacterium mosquito attractant is housed in the population cage in the described step (3).