CN103392506A - Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method - Google Patents
Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method Download PDFInfo
- Publication number
- CN103392506A CN103392506A CN2013103392607A CN201310339260A CN103392506A CN 103392506 A CN103392506 A CN 103392506A CN 2013103392607 A CN2013103392607 A CN 2013103392607A CN 201310339260 A CN201310339260 A CN 201310339260A CN 103392506 A CN103392506 A CN 103392506A
- Authority
- CN
- China
- Prior art keywords
- liquid
- mycelium
- ganoderma lucidum
- fermentation
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000000855 fermentation Methods 0.000 title claims abstract description 27
- 230000004151 fermentation Effects 0.000 title claims abstract description 27
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 23
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 22
- VQWZVMRSWMNBDB-UHFFFAOYSA-N [B].[Se].[Zn].[Mn].[Cr] Chemical compound [B].[Se].[Zn].[Mn].[Cr] VQWZVMRSWMNBDB-UHFFFAOYSA-N 0.000 title abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims abstract description 6
- 239000000725 suspension Substances 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 3
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 3
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 13
- 239000011573 trace mineral Substances 0.000 claims description 13
- 235000013619 trace mineral Nutrition 0.000 claims description 13
- 229910052804 chromium Inorganic materials 0.000 claims description 12
- 239000011651 chromium Substances 0.000 claims description 12
- FXQVXDWQPZPZNA-UHFFFAOYSA-N selanylideneboron Chemical compound [Se]=[B] FXQVXDWQPZPZNA-UHFFFAOYSA-N 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 8
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 239000011701 zinc Substances 0.000 claims description 6
- 229910052725 zinc Inorganic materials 0.000 claims description 6
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 239000011669 selenium Substances 0.000 claims description 5
- 229910052711 selenium Inorganic materials 0.000 claims description 5
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 4
- 229910052796 boron Inorganic materials 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 239000011572 manganese Substances 0.000 claims 1
- 229910052748 manganese Inorganic materials 0.000 claims 1
- 230000004913 activation Effects 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 238000012807 shake-flask culturing Methods 0.000 abstract 2
- 239000008213 purified water Substances 0.000 abstract 1
- 241000222336 Ganoderma Species 0.000 description 15
- 241000207961 Sesamum Species 0.000 description 5
- 235000003434 Sesamum indicum Nutrition 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 150000003648 triterpenes Chemical class 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 3
- 230000000144 pharmacologic effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 2
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 2
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 2
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 229910017052 cobalt Inorganic materials 0.000 description 2
- 239000010941 cobalt Substances 0.000 description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 2
- 239000011737 fluorine Substances 0.000 description 2
- 229910052731 fluorine Inorganic materials 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 150000002596 lactones Chemical class 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
- 229910052750 molybdenum Inorganic materials 0.000 description 2
- 239000011733 molybdenum Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001603 reducing effect Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- GOLORTLGFDVFDW-UHFFFAOYSA-N 3-(1h-benzimidazol-2-yl)-7-(diethylamino)chromen-2-one Chemical compound C1=CC=C2NC(C3=CC4=CC=C(C=C4OC3=O)N(CC)CC)=NC2=C1 GOLORTLGFDVFDW-UHFFFAOYSA-N 0.000 description 1
- 108091005508 Acid proteases Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001489091 Ganoderma sinense Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FBPFZTCFMRRESA-BXKVDMCESA-N L-mannitol Chemical compound OC[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)CO FBPFZTCFMRRESA-BXKVDMCESA-N 0.000 description 1
- 229930182842 L-mannitol Natural products 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 240000001307 Myosotis scorpioides Species 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000009697 arginine Nutrition 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007012 clinical effect Effects 0.000 description 1
- 229960000956 coumarin Drugs 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 150000002240 furans Chemical class 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical class [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000002443 hepatoprotective effect Effects 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 235000005772 leucine Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000011707 mineral Chemical class 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 235000008729 phenylalanine Nutrition 0.000 description 1
- 229960005190 phenylalanine Drugs 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000011127 radiochemotherapy Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- WBHHMMIMDMUBKC-QJWNTBNXSA-N ricinoleic acid Chemical compound CCCCCC[C@@H](O)C\C=C/CCCCCCCC(O)=O WBHHMMIMDMUBKC-QJWNTBNXSA-N 0.000 description 1
- 229960003656 ricinoleic acid Drugs 0.000 description 1
- 229910052701 rubidium Inorganic materials 0.000 description 1
- IGLNJRXAVVLDKE-UHFFFAOYSA-N rubidium atom Chemical compound [Rb] IGLNJRXAVVLDKE-UHFFFAOYSA-N 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- RXHIKAIVEMAPRU-JRIGQVHBSA-N sequiterpene Natural products C1=C(C)[C@@H](OC(C)=O)[C@H](O)[C@@]2(O)[C@H](C)CC[C@@H](C(C)=C)[C@H]21 RXHIKAIVEMAPRU-JRIGQVHBSA-N 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 229910052718 tin Inorganic materials 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium atom Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method. The chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method comprises the following steps that a bacterial strain is transferred to a slope medium to carry out cultivation and activation, and a slope strain is obtained; the slope strain is manufactured into spore suspension to be received into a liquid medium, shake-flask culture is conducted, and a liquid strain is obtained; high-temperature sterilization is conducted on a fermentation medium and then cooling is conducted on the fermentation medium, the liquid strain is received, shake-flask culture is conducted, and fermentation broth of the mycelium is obtained; the mycelium and the fermentation broth are separated through filtering, the mycelium is washed by purified water, the medium is washed away, the mycelium is collected and is baked to be in a constant weight, and a dry mycelium body is obtained. The chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method is characterized in that microelements are added into the fermentation medium, can improve the content of microelements in the ganoderma lucidum mycelium, and is easy to operate, low in cost and suitable for industrial production.
Description
Technical field
The present invention relates to the fungi fermentation engineering, in particular to a kind of production method of rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation.
Background technology
Glossy ganoderma claims again polyporus lucidus, refreshing sesame, sesame grass, celestial grass, auspicious grass, is the complete stool of the red sesame of Polyporaceae plant or purple sesame.Take the Ganoderma Sinense drug effect as best, glossy ganoderma originates in east Asia, distribution in China the widest in Jiangxi, glossy ganoderma is as the China's tradition valuable ingredient of traditional Chinese medicine that has thousands of years medicinal histories, possesses very high medical value, modern pharmacology through the scientific research institution many decades studies confirm that, glossy ganoderma, for strengthening body immunity, is regulated blood sugar, control blood pressure, auxiliary tumor chemoradiotherapy, liver protecting, the aspects such as promotion sleep all have significant curative effect.
People have carried out a large amount of research to the pharmacological action of glossy ganoderma, confirm that it has pharmacological activity and clinical effect widely.Through a large amount of studies have shown that, the mycelium that Ganoderma lucidum submerged fermentation is produced and the composition fairly similar of natural glossy ganoderma, and proved that it has similar pharmacological action and clinical effectiveness to natural glossy ganoderma,
The glossy ganoderma chemical composition mainly contains ergosterol 0.3%～0.4%, fungal lysozyme and acid protease, L-mannitol.In water-soluble extracting liquid, contain water soluble protein, several amino acids, polypeptide and the polysaccharoses such as asparatate, glutamic acid, arginine, lysine, leucine, alanine, tryptophan, threonine, proline, methionine, phenyl alanine, serine.Still resinous, lactone, coumarin etc.
Purple sesame contains ergosterol, and organic acid is ricinic acid, fumaric acid.In addition, and contain amino acid, glucose, polysaccharose, resin and mannitol.General glossy ganoderma has the reactions such as alkaloid, sterol, lactone, coumarin, acidic resins, amino acid, grease, reducing substances.
The chemical composition of Ganoderma is comparatively complicated, and because of bacterial classification used, the bacterial classification place of production, cultivation method, extraction process, formulation method different different.In the fruit body of Ganoderma, mycelium and spore, contain polysaccharose, ucleosides, furan derivative, steroid ferment class, alkaloids, protein, polypeptide, amino acids, triterpenes, sequiterpene, organic germanium, mineral salt etc.GL-B is one of main active ingredient of glossy ganoderma, has antitumor, immunological regulation, hypoglycemic, anti-oxidant, reducing blood lipid and anti-aging effects.Not lower over one hundred kind of the contained triterpenes of glossy ganoderma, wherein take tetracyclic triterpenes as main, the bitter taste of glossy ganoderma is relevant with contained triterpenes.Triterpenes is also one of active ingredient of glossy ganoderma, and human liver cancer cell is had to cytotoxicity, also can suppress the release of histamine, has hepatoprotective effect and has anti-allergic effects etc.
Human total weight's ten thousand/following element, as zinc, copper, manganese, chromium, selenium, molybdenum, cobalt, fluorine etc., be called trace element.The content of trace element in human body is really very little, as zinc, only accounts for human total weight's 1,000,000/33.
Up to the present, being identified the essential trace element relevant with health and life has 18 kinds, and iron, copper, zinc, cobalt, manganese, chromium, selenium, iodine, nickel, fluorine, molybdenum, vanadium, tin, silicon, strontium, boron, rubidium, arsenic etc. are namely arranged.This every kind trace element has its special physiological function.Although they are minimum at people's in-vivo content, they are very necessary to some conclusive metabolism of remaining in human body.In case lacked these essential trace elements, human body just there will be disease, even threat to life.
It is imperative that thereby the improvement by production technology increases substantially content and the kind of trace element in ganoderma lucidum mycelium.
Summary of the invention
The object of the present invention is to provide a kind of method of rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation, to overcome the low and single defect of micronutrient levels in the prior art ganoderma lucidum mycelium.
After systematic research and test, proposed to improve by adding trace element the production method of the output of Ganoderma lucidum submerged fermentation Chinese caterpillar fungus hypha body weight trace element, method comprises:
(1) bacterial strain is transferred and on slant medium, cultivate, activate, obtain slant strains;
(2) slant strains is made to spore suspension, be connected in liquid nutrient medium, shaking flask is cultivated, and obtains liquid spawn;
(3) cooling after the fermentation medium high-temperature sterilization, then by the liquid spawn access, shaking flask is cultivated, and obtains containing mycelial zymotic fluid;
(4) by filtering mycelium and separation of fermentative broth, the purified rinse water mycelia, wash away medium, collects mycelium, dries to constant weight, and makes dried mycelium.
In the second step of described method, liquid spawn culture medium formula used is micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
In the second step of described method, process conditions used are: the liquid spawn culture medium liquid amount is the 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ° of C, rotating speed 160r/min.
In the 3rd step of described method, culture medium prescription used is micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
In the 3rd step of described method, process conditions used are: liquid amount 200mL/500mL triangular flask, and adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, under 23 ℃, shake the 150r/min rotating speed and cultivate 10d.
The ratio of described trace elements of selenium element, chromium element, zinc element, manganese element and boron element is: 10: 10: 3: 3: 1.Key technology of the present invention is in fermentation medium, adds simultaneously selenium element, chromium element, zinc element, manganese element and boron element.
The present invention has improved the content of trace element in the ganoderma lucidum mycelium, and then improves the drug effect of ganoderma lucidum mycelium, and significant, the mycelia of production can be used as functional food, health products, the raw material of medicine; This method is easy to operate, and cost is low, is suitable for industrial production.
Embodiment
Embodiment 1: bacterial strain is transferred and under on the PDA slant medium 25 ℃, cultivated 7d, activation 2 times, obtain slant strains; Scrape gently and wash slant strains and it is made to spore suspension, then use sterile gauze (6 layers) to filter out the mycelia in suspension, every bottle of moderate Reishi sporule of liquid spawn access concentration, make the mycelium pellet in the liquid of making tiny and even, the zymotic fluid clear.The liquid spawn culture medium formula is: micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8, liquid spawn culture medium liquid amount are the 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ° of C, rotating speed 160r/min, obtain liquid spawn.
Use optimized fermentation medium: micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.Process conditions used are: liquid amount 200mL/500mL triangular flask, and adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, under 23 ℃, shake the 150r/min rotating speed and cultivate 10d.Obtain the zymotic fluid that most of mycelium morphology is mycelium pellet; After filtration by mycelium and separation of fermentative broth, then use the purified rinse water mycelia, after washing away medium, collect mycelium, dry to constant weight.
Claims (6)
1. the method for a rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation, it is characterized in that: the method comprises following four steps: (1) is transferred bacterial strain and is cultivated on slant medium, activates, and obtains slant strains; (2) slant strains is made to spore suspension, be connected in liquid nutrient medium, shaking flask is cultivated, and obtains liquid spawn; (3) cooling after the fermentation medium high-temperature sterilization, then by the liquid spawn access, shaking flask is cultivated, and obtains containing mycelial zymotic fluid; (4) by filtering mycelium and separation of fermentative broth, the purified rinse water mycelia, wash away medium, collects mycelium, dries to constant weight, and makes dried mycelium.Key problem in technology is in fermentation medium, to add trace element.We's Faxian can improve the content of trace element in ganoderma lucidum mycelium.
2. the method for fermenting according to rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid claimed in claim 1, it is characterized in that: in the second step of described method, liquid spawn culture medium formula used is: micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
3. according to the method for rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation claimed in claim 1, it is characterized in that: in the second step of described method, process conditions used are: the liquid spawn culture medium liquid amount is the 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ℃, rotating speed 160r/min.
4. according to the method for rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation claimed in claim 1, it is characterized in that: in the 3rd step of described method, culture medium prescription used is micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
5. the method for fermenting according to rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid claimed in claim 1, it is characterized in that: in the 3rd step of described method, process conditions used are: liquid amount 200mL/500mL triangular flask, adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, under 23 ℃, shake the 150r/min rotating speed and cultivate 10d.
6. according to the method for rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation claimed in claim 1, it is characterized in that: the ratio of described trace elements of selenium, chromium, zinc, manganese and boron is: 10: 10: 3: 3: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103392607A CN103392506A (en) | 2013-08-02 | 2013-08-02 | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103392607A CN103392506A (en) | 2013-08-02 | 2013-08-02 | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103392506A true CN103392506A (en) | 2013-11-20 |
Family
ID=49556421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103392607A Pending CN103392506A (en) | 2013-08-02 | 2013-08-02 | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103392506A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834138A (en) * | 2017-02-16 | 2017-06-13 | 江苏中祥高科技实业有限公司 | A kind of production method of zinc-rich ganoderma lucidum mycelium |
| CN108522145A (en) * | 2018-05-11 | 2018-09-14 | 福建省农业科学院农业质量标准与检测技术研究所 | A kind of white fungus culture medium and cultivating white fungus method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358413A (en) * | 2000-10-06 | 2002-07-17 | 宋在澫 | Method for preparing mushroom mycelium and use |
| CN101755615A (en) * | 2009-12-31 | 2010-06-30 | 张寿禄 | Cultivation method of high trace element edible and medical fungi |
| CN102475033A (en) * | 2010-11-24 | 2012-05-30 | 金勇� | Method for producing ganoderma lucidum mycelium rich in selenium |
| CN102972211A (en) * | 2012-12-21 | 2013-03-20 | 徐州工程学院 | Method for increasing growth speed of ganoderma lucidum mycelia and liquid fermentation biomass |
-
2013
- 2013-08-02 CN CN2013103392607A patent/CN103392506A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358413A (en) * | 2000-10-06 | 2002-07-17 | 宋在澫 | Method for preparing mushroom mycelium and use |
| CN101755615A (en) * | 2009-12-31 | 2010-06-30 | 张寿禄 | Cultivation method of high trace element edible and medical fungi |
| CN102475033A (en) * | 2010-11-24 | 2012-05-30 | 金勇� | Method for producing ganoderma lucidum mycelium rich in selenium |
| CN102972211A (en) * | 2012-12-21 | 2013-03-20 | 徐州工程学院 | Method for increasing growth speed of ganoderma lucidum mycelia and liquid fermentation biomass |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834138A (en) * | 2017-02-16 | 2017-06-13 | 江苏中祥高科技实业有限公司 | A kind of production method of zinc-rich ganoderma lucidum mycelium |
| CN108522145A (en) * | 2018-05-11 | 2018-09-14 | 福建省农业科学院农业质量标准与检测技术研究所 | A kind of white fungus culture medium and cultivating white fungus method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103263448B (en) | Fermentation bacteria used for fermentation pretreatment to improve extraction of Ginkgo biloba L. leaf flavone and application | |
| CN102936609B (en) | Preparation method of swift moth paecilomyces varioti extracellular acid glycoprotein | |
| CN103073651B (en) | Ganoderan extraction method and ganoderan use | |
| CN101143002A (en) | Deep liquid fermentation method for preparing selenium-rich gold needle mushroom crude polysaccharide powder | |
| CN103316058A (en) | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation | |
| CN103130909A (en) | Preparation method of selenium-rich Morchella polysaccharide | |
| CN103509091B (en) | A kind of Grifola frondosa mycelium anti-tumor glycoprotein and preparation method | |
| CN103073648A (en) | Method for increasing antioxidation activity of flammulina velutipes polysaccharide | |
| CN105193905A (en) | Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation | |
| CN105724047B (en) | A kind of cultural method of high yield and high quality selenium-rich Cordceps militaris | |
| CN105925635A (en) | Production process of icariside II | |
| CN112791108B (en) | A kind of preparation method of Phellinus linteus active ingredient and its application in auxiliary hypoglycemic drugs | |
| CN105368895A (en) | Method for preparing dinghu scale toadstool intracellular and extracellular polysaccharide with antioxidant activity | |
| CN102925527A (en) | Method for mixing and fermenting flammulina velutipes and lucid ganoderma | |
| AU2020102037A4 (en) | A method of efficiently increasing the alpha-glucosidase inhibitor content in fresh mulberry leaves by the solid-state fermentation | |
| CN102191183A (en) | Morchella submerged fermentation production process | |
| KR20130011511A (en) | Fermented ginseng food manufacturing method | |
| CN103404855B (en) | Grifola frondosa health care oral solution and preparation method thereof | |
| CN106148200A (en) | The low lead of a kind of selenium-rich, the culture medium of Cordyceps mycelium of arsenic and inoculation method thereof | |
| Zhang et al. | Enhancement of diosgenin production in Dioscorea zingiberensis cell culture by oligosaccharide elicitor from its endophytic fungus Fusarium oxysporum Dzf17 | |
| CN105213461A (en) | Utilize the two-way fermentation of Ganoderma-Radix Codonopsis to improve the biotechnology of its drug effect | |
| CN103392506A (en) | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method | |
| CN103650920A (en) | Method for fermenting ganoderma lucidum mycelia liquid rich in microelements | |
| CN104278070A (en) | Method for improving content of ergosterol in liquid fermentation products of phellinus igniarius | |
| CN102533900A (en) | Method for extracting lentinan |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131120 |