CN103750563A - Preparation method and radical reduction and harm reduction application of squalene and tea polyphenol composition liquid proliposome - Google Patents

Abstract

The invention discloses a preparation method and radical reduction and harm reduction application of squalene and tea polyphenol composition liquid proliposome. The liquid proliposome is prepared from 7-10 parts of hydrogenated phospholipid, 8-12 parts of stabilizer, 10-15 parts of surfactant, 25-45 parts of squalene and tea polyphenol composition and the balance water. The liquid proliposome is filled into a hollow cylinder with waterproof dustproof gas-permeable membranes at two ends, and the hollow cylinder is disposed inside a cigarette filter in the forming process of a cigarette filter rod to obtain the cigarette filter capable of reducing cigarette harm. When a consumer smokes, smoke passes the internally-disposed liquid proliposome, an antioxidant and main stream smoke are in full contact since the liquid proliposome is large in surface area, gas-phase free radicals in the main stream smoke can be effectively eliminated, and strong technical support is provided for solving the problem about smoking and health.

Description

Preparation method of squalene and tea polyphenols composition liquid proliposome and its application in base reduction and harm reduction

technical field

The invention relates to the field of chemical technology, more specifically, to a liquid proliposome containing a composition of squalene and tea polyphenols, a preparation method thereof, and an application in reducing bases and harms of hookah-type filters.

Background technique

Thousands of compounds produced by smoking and the harm caused by high concentration of free radicals to the human body have attracted widespread attention. Free radicals have the strongest effect on the body and are the most harmful. They can cause lung disease, heart disease and cancer. These free radicals can cause serious damage to smokers. Smoking produces high concentrations of free radicals, including relatively long-lived solid-phase free radicals, quinones and semiquinones, polycyclic aromatic hydrocarbons free radicals, and reactive oxygen free radicals produced by their reactions with oxygen such as superoxide anion (O ₂ ^- ), hydrogen peroxide (H ₂ O ₂ ), hydroxyl radicals (·OH), singlet oxygen ( ¹ O ₂ ) and lipid peroxidation radicals (ROO·) and gas phase free radicals with a relatively short existence time radicals, alkoxy radicals (RO·), alkyl radicals (R·), nitrogen oxide radicals, etc.

At present, there are not many researches on the scavenging of free radicals in flue gas, mainly from the addition of natural antioxidants, vitamins, amino acids, proteins, etc., as well as biological enzyme free radical scavengers, trace elements, and natural minerals to reduce free radicals in flue gas human injury. In addition, some areas of our country have the habit of smoking hookah, which means that the burned smoke passes through the water in the hookah and then inhales into the mouth. It can not only remove harmful chemicals dissolved in water, but also remove some gaseous free radicals. Reduced the harm of smoking to the human body. And it can reduce the probability of human body getting angry due to smoking. Luo Fuzhong (2013) disclosed a utility model patent with the publication number CN 203182005 U to disclose a hookah-type filter tip, adding surfactants and/or extracts of Chinese herbal medicines such as mint and licorice into the water, the purpose of which is to significantly reduce tar, Nicotine content, will not get angry, and will not affect the taste of tobacco. improve the design. Jiang Huaguo (1998) disclosed a portable water pipe in his utility model patent application, which simplifies the structure of the water pipe; Huang Hui (2005) disclosed a water pipe using For the method of decoking and comprehensive harm reduction of cigarettes, the liquid preparation formulated for decoking and comprehensive harm reduction is studied. Among the above-mentioned hookah-type filters, there is no research on scientifically adding suitable antioxidants into the hookah-type cigarette holder to remove gas-phase free radicals in the smoke.

Squalene is a lipid unsaponifiable substance, a polyunsaturated olefin (tricosahexaene), originally discovered from shark liver oil, named Squalene in 1914, and its chemical name is 2, 6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, which is an open-chain triterpene, also known as cod liver oil terpene, participates in the synthesis of cholesterol in the human body and can It promotes metabolism, has many physiological functions such as increasing the activity of superoxide dismutase (SOD) in the body, enhancing the body's immunity, anti-aging, anti-fatigue, anti-tumor, etc. It is a non-toxic marine organism with the effect of preventing and curing diseases active substance. Squalene is widely distributed in plants, but the content is not high, mostly less than 5% of the unsaponifiable matter in vegetable oil, and only a few have more content, such as olive oil containing squalene 150-700mg/100g, rice bran oil containing The content is 332mg/100g.

Tea polyphenols are the general term for catechins, acetones, phenolic acids and anthocyanins in tea. It is a fused-ring aromatic hydrocarbon, which can be divided into flavanols, hydroxy-[4]-flavanols, anthocyanins, flavonoids, flavonols and phenolic acids. Among them, catechins are the most important, accounting for about 60% to 80% of the total polyphenols; catechins are mainly composed of several monomers such as EGC, DLC, EC, EGCG, GCG, and ECG. The content of tea polyphenols in tea is generally 20-35%. Among the components of tea polyphenols, flavanols are the main components, and flavanols are mainly catechins. The content of catechins accounts for about 70% of the total amount of tea polyphenols. It has a strong effect of eliminating harmful free radicals, anti-aging effect, anti-radiation effect, inhibitory effect on cancer cells, antibacterial and bactericidal effects, and inhibitory effect on HIV. Existing studies have proved that tea polyphenols are also good for smokers , can clean the lungs of nicotine.

In order to overcome the shortcomings of common antioxidants and achieve the purpose of simultaneously removing long-lived solid-phase free radicals and relatively short-lived gas-phase free radicals, the applicant has devoted himself to related research for a long time and obtained related patents. In the application with the patent number ZL201010529711.X, the composition of squalene and tea polyphenols and its application in cigarettes are disclosed. The synergistic composition of squalene and tea polyphenols is directly added to shredded tobacco to obtain better application effect. However, further studies have found that squalene and tea polyphenols are prone to oxidative deterioration in the air, and the composition is unstable during application, which is inconvenient for actual production.

Liposome is an artificial membrane. When amphiphilic molecules such as phospholipids and sphingolipids are dispersed in the aqueous phase, the hydrophobic tails of the molecules tend to gather together and avoid the aqueous phase, while the hydrophilic heads are exposed to the aqueous phase, forming closed vesicles with a bilayer structure , called liposomes. According to structural classification, liposomes are divided into unilamellar liposomes, multilamellar liposomes and liquid proliposomes. Liquid proliposome is a kind of liposome with sustained release effect, composed of many non-concentric ventricular connections, with a particle size of 5-50 μm, which has a larger encapsulation volume than ordinary liposomes, and is especially suitable for water-soluble Encapsulation of sexual drugs. The application of liposome is mainly in medicine at present.

The research of processing these free radical scavengers into liquid proliposomes and scientifically placing them in hookah cigarette holders to scavenge gaseous free radicals (reduced radicals) has not been reported at present, let alone studies based on the enhancement of squalene and tea polyphenols. Preparation technology and application report of liquid proliposomes with effective composition.

Contents of the invention

The purpose of the present invention is to overcome the deficiency in the application of existing liquid proliposome technology in cigarettes, and the technical deficiency in the application of squalene and tea polyphenol synergistic composition in cigarettes, and provide a kind of Liquid proliposomes of tea polyphenol compositions.

Another object of the present invention is to provide a method for preparing the liquid proliposome.

Still another object of the present invention is to provide the application of the liquid proliposome containing squalene and tea polyphenols composition in hookah filter.

The purpose of the present invention is achieved through the following technical solutions:

A liquid proliposome containing squalene and tea polyphenol composition is provided, and the liquid proliposome is prepared from the following components in parts by mass:

Hydrogenated phospholipids: 7 to 10 parts;

Stabilizer: 8 to 12 parts;

Surfactant: 10-15 parts;

Composition of squalene and tea polyphenols: 25-45 parts;

remaining water;

The sum of the mass parts of the above-mentioned components is 100 parts;

Preferably, the liquid proliposome containing squalene and tea polyphenol composition is prepared from the following components in parts by mass:

Hydrogenated phospholipids: 8-9 parts;

Stabilizer: 9 to 11 parts;

Surfactant: 12 to 14 parts;

Composition of squalene and tea polyphenols: 30-35 parts;

remaining water;

The sum of the parts by mass of the above-mentioned components is 100 parts.

The liquid proliposome containing the composition of squalene and tea polyphenol can be prepared by using the above-mentioned components by double emulsion method.

The phospholipids are one or more of lecithin, soybean lecithin, and hydrogenated soybean lecithin; a variety of phospholipids are used, and the mixing ratio of various phospholipids is not strictly limited.

The stabilizer is preferably hydroxystarch, more preferably, the hydroxystarch is one or more of hydroxymethyl starch, hydroxyethyl starch or hydroxypropyl starch. When using multiple types, the mixing ratio is not strictly limited.

The surfactant is glycerol.

The mass ratio of squalene and tea polyphenols in the squalene and tea polyphenols composition is 1:8-8:1, and the more optimal ratio is: 2:7-7:2.

The squalene can be an extract extracted from plants, animals or microorganisms or a purified product, and commercially available products can be used.

The tea polyphenols can be tea polyphenol extracts extracted from tea leaves or purified pure products, and commercially available products can be used.

The present invention also provides the preparation method of the liquid proliposome containing squalene and tea polyphenols composition, scientifically compatible raw materials, accurate process conditions, and overcomes the incompatibility of squalene and tea polyphenols in water solubility.

High disadvantages, the successful preparation of liquid proliposomes containing squalene and tea polyphenols mixed synergistic composition can well achieve the purpose of applying it to hookah filters. The preparation method comprises the following steps:

S1. Dissolving the composition of phospholipids, squalene and tea polyphenols in glycerol to obtain a mixed system;

S2. Homogenize the mixed system obtained in S1 at high speed to make it fully mixed to obtain a homogenized mixed system;

S3. Mix the stabilizer and water to obtain an aqueous solution of the stabilizer, add the aqueous solution of the stabilizer to the homogenized mixing system obtained in S2 and stir evenly to form a stable particle suspension to obtain the composition containing squalene and tea polyphenols liquid proliposomes.

Preferably, the process conditions of the high-speed homogenization described in S2 are: 2000 r/min, stirring for 45min.

The invention provides the liquid proliposome containing squalene and tea polyphenols prepared by the above method.

The liquid proliposome containing squalene and tea polyphenol composition of the present invention is very well applied in cigarettes, and is particularly applied to a hookah filter tip, and the liquid proliposome is packed into In a hollow cylinder with waterproof, dustproof and air-permeable membranes at both ends, it is built into a cigarette filter tip of acetate fiber during the molding process of a cigarette filter rod.

The preferred application method is: the liquid proliposome is packed into a hollow cylinder with waterproof, dustproof and air-permeable membranes at both ends, and 1 gram of the liquid proliposome is packed into each capsule, and placed in the cigarette filter In the rod forming process, it is built into the acetate fiber cigarette filter. Each cigarette filter is built with 1 capsule, and the cigarette filter that can reduce the harm of cigarettes is obtained. When consumers smoke, the smoke passes through the built-in liquid precursor liposome, due to the liquid The proliposome has a large surface area, and the antioxidant is in full contact with the mainstream smoke, which can effectively remove the gas-phase free radicals in the mainstream smoke, and provide strong technical support for solving the problems of smoking and health.

Beneficial effects of the present invention:

The present invention applies liposome technology, especially liquid proliposome technology, to cigarettes for the first time, and successfully combines squalene and tea polyphenol synergistic composition, sums up suitable compatibility aids and compatibility ratio, simple and easy The liquid proliposomes containing squalene and tea polyphenols synergistic composition were successfully prepared, which provided strong technical support for the further development and application of liposomes. The obtained liquid proliposomes were highly dispersed micro-liquids Drops, high dispersion, large specific surface area, can be directly applied to hookah filter.

The invention successfully embeds the synergistic composition in the liquid proliposome, overcomes the shortcoming that squalene and tea polyphenols are easy to oxidize and fail in air, and simultaneously overcomes the synergistic composition of squalene and tea polyphenols The defect of being insoluble in water, by microencapsulating the compounded synergistic composition of squalene and tea polyphenols, the stability of the compounded synergistic composition of squalene and tea polyphenols can be increased, and it can be applied to cigarette filters liquid proliposomes.

The invention precisely defines the raw material compatibility and preparation process conditions, and the processed liquid proliposome has strong air permeability and large surface area, which is suitable for the application requirements of cigarette filters, can be directly applied to hookah filters, and the antioxidant can be fully mixed with mainstream smoke. Contact, to achieve the purpose of removing free radicals in mainstream smoke, and provide strong technical support for solving the problems of smoking and health.

Detailed ways

The present invention will be further described in detail below in conjunction with specific examples. The test methods used in the following examples, unless otherwise specified, are conventional methods; the materials, reagents, etc. used, unless otherwise specified, are reagents and materials that can be obtained from conventional commercial channels.

Example 1

Prepare each component according to the following parts by mass: 10 grams (g) of hydrogenated lecithin; squalene (Shanghai Xinran Biotechnology Co., Ltd., with a content greater than 96.0%, the same as the following examples. But this does not limit the present invention, Those skilled in the art can use other sources of squalene) 5 grams (g); tea polyphenols (Ningbo Youjie Chemical Co., Ltd., content greater than 98.0%, the following examples are the same. But it does not therefore limit the present invention, the present invention Those skilled in the art can use 20 grams (g) of tea polyphenols from other sources; 10 grams (g) of glycerol; 8 grams (g) of hydroxymethyl starch, and 47 grams (g) of water.

The preparation steps are:

S1. Dissolving the composition of hydrogenated lecithin, squalene and tea polyphenols in glycerol to obtain a mixed system;

S2. Homogenize the mixed system obtained in S1 at high speed to make it fully mixed to obtain a homogenized mixed system;

S3. Mix the stabilizer and water to obtain an aqueous solution of the stabilizer, add the aqueous solution of the stabilizer to the homogenized mixing system obtained in S2 and stir evenly to form a stable particle suspension to obtain the composition containing squalene and tea polyphenols liquid proliposomes.

Example 2

The preparation steps are the same as in Example 1. Weigh 10 grams of hydrogenated soybean lecithin, 10 grams of squalene, 15 grams of tea polyphenols dissolved in 10 grams of glycerol, homogenize at high speed to make it fully mixed, then add 8 grams of hydroxyethyl starch dissolved in 47 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 3

The preparation steps are the same as in Example 1. Weigh 10 grams of hydrogenated soybean lecithin, 15 grams of squalene, 10 grams of tea polyphenols dissolved in 10 grams of glycerol, homogenize at high speed to make it fully mixed, then add 8 grams of hydroxymethyl starch dissolved in 47 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 4

The preparation steps are the same as in Example 1. Weigh 10 grams of hydrogenated soybean lecithin, 5 grams of squalene, 20 grams of tea polyphenols dissolved in 10 grams of glycerin, homogenize at high speed to make it fully mixed, then add 8 grams of hydroxypropyl starch dissolved in 47 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 5

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated lecithin, 5 grams of squalene, and dissolve 25 grams of tea polyphenols in 12 grams of glycerol, homogenize at a high speed to fully mix, then add 10 grams of hydroxyethyl starch and dissolve in 40 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 6

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean lecithin, 10 grams of squalene, 20 grams of tea polyphenols dissolved in 12 grams of glycerin, homogenize at a high speed to fully mix, then add 10 grams of hydroxypropyl starch dissolved in 40 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 7

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean lecithin, 15 grams of squalene, and 15 grams of tea polyphenols dissolved in 12 grams of glycerin, homogenize at a high speed to fully mix, then add 10 grams of hydroxymethyl starch dissolved in 40 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 8

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean phospholipids, 20 grams of squalene, 10 grams of tea polyphenols dissolved in 12 grams of glycerin, homogenize at a high speed to fully mix, then add 10 grams of hydroxyethyl starch dissolved in 40 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 9

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean lecithin, 25 grams of squalene, 5 grams of tea polyphenols dissolved in 12 grams of glycerin, homogenize at high speed to make it fully mixed, then add 10 grams of hydroxypropyl starch dissolved in 40 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 10

The preparation steps are the same as in Example 1. Weigh 7 grams of hydrogenated soybean lecithin, 5 grams of squalene, and dissolve 35 grams of tea polyphenols in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 12 grams of hydroxypropyl starch dissolved in 26 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 11

The preparation steps are the same as in Example 1. Weigh 7 grams of hydrogenated lecithin, 10 grams of squalene, and dissolve 30 grams of tea polyphenols in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 12 grams of hydroxyethyl starch dissolved in 26 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 12

The preparation steps are the same as in Example 1. Weigh 7 grams of hydrogenated lecithin, 15 grams of squalene, and dissolve 25 grams of tea polyphenols in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 12 grams of hydroxymethyl starch and dissolve in 26 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 13

The preparation steps are the same as in Example 1. Weigh 7 grams of hydrogenated lecithin, 20 grams of squalene, and 20 grams of tea polyphenols dissolved in 15 grams of glycerin, homogenize at a high speed to fully mix, then add 12 grams of hydroxymethyl starch dissolved in 26 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 14

The preparation steps are the same as in Example 1. Weigh 7 grams of hydrogenated soybean lecithin, 30 grams of squalene, 10 grams of tea polyphenols dissolved in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 12 grams of hydroxyethyl starch dissolved in 26 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 15

The preparation steps are the same as in Example 1. Weigh 7 grams of hydrogenated lecithin, 35 grams of squalene, 5 grams of tea polyphenols dissolved in 15 grams of glycerin, homogenize at a high speed to fully mix, then add 12 grams of hydroxypropyl starch dissolved in 26 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 16

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean lecithin, 5 grams of squalene, 40 grams of tea polyphenols dissolved in 15 grams of glycerin, homogenize at high speed to make it fully mixed, then add 10 grams of hydroxymethyl starch dissolved in 22 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 17

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean phospholipids, 10 grams of squalene, 35 grams of tea polyphenols dissolved in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 10 grams of hydroxyethyl starch dissolved in 22 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 18

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean lecithin, 20 grams of squalene, and 25 grams of tea polyphenols dissolved in 15 grams of glycerin, homogenize at a high speed to fully mix, then add 10 grams of hydroxypropyl starch dissolved in 22 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 19

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated lecithin, 30 grams of squalene, and dissolve 15 grams of tea polyphenols in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 10 grams of hydroxymethyl starch and dissolve in 22 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 20

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated soybean phospholipids, 35 grams of squalene, 10 grams of tea polyphenols dissolved in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 10 grams of hydroxymethyl starch dissolved in 22 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

Example 21

The preparation steps are the same as in Example 1. Weigh 8 grams of hydrogenated lecithin, 40 grams of squalene, 5 grams of tea polyphenols dissolved in 15 grams of glycerol, homogenize at a high speed to fully mix, then add 10 grams of hydroxymethyl starch dissolved in 22 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained.

The detection results of the liquid proliposomes prepared in the above-mentioned Examples 1-21 are as follows: detection by laser light scattering method, the particle size of the liquid proliposomes prepared in Examples 1-21 is 65-100 nm, and the distribution is uniform. It is normal and the span is small.

The free squalene in the solution was separated from tea polyphenols and liposomes by dialysis separation method, and the encapsulation efficiency was measured and calculated respectively. The encapsulation efficiency was calculated according to (mass of squalene and tea polyphenol composition in liposome/total mass of squalene and tea polyphenol composition in liposome)×100%. The encapsulation efficiency of the liquid proliposomes prepared in Examples 1-21 of the present invention is above 88%.

Comparative example 1

The preparation steps are the same as in Example 1. Weigh 5 grams of hydrogenated lecithin, 40 grams of squalene, 5 grams of tea polyphenols dissolved in 20 grams of glycerol, homogenize at a high speed to fully mix, then add 5 grams of hydroxymethyl starch dissolved in 25 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained. The particle size of the prepared liquid proliposome is 190-370 nm, and the distribution is uneven. The encapsulation efficiency was 57%.

Comparative example 2

Weigh 7 grams of cephalin, 35 grams of squalene, and dissolve 5 grams of tea polyphenols in 15 grams of propylene glycol, homogenize at a high speed to fully mix, then add 12 grams of hydroxypropyl methylcellulose dissolved in 26 grams of water The aqueous solution is stirred evenly to form a stable particle suspension, and the liquid proliposome containing the antioxidant squalene and tea polyphenol composition is obtained. The particle size of the prepared liquid proliposome is 310-580 nm, and the distribution is uneven. The encapsulation efficiency was 41%.

Example 22 Application experiment

The liquid proliposomes of the above-mentioned Examples 1-21 are packed into hollow cylinders with waterproof, dustproof and gas-permeable membranes at both ends, and each capsule is filled with 1 gram. During the forming process of the cigarette filter tip, it is placed in the cigarette filter tip of acetate fiber, and one piece is built in each cigarette filter tip to obtain a hookah-type filter tip capable of reducing the damage of free radicals in the gas phase of the cigarette. A 100% liquid proliposome without squalene or tea polyphenols was used as a control.

Detection of free radical scavenging effect in cigarette smoke: free radicals in smoke gas phase were captured by spin trapping agent PBN (2-phenyl-tert-butylnitrone) and detected.

The conventional gas-phase free radical detection method is adopted: glassware is used to collect smoke free radicals. Add a certain concentration of carbon tetrachloride solution of the free radical scavenger PBN to the collector, connect the collector to the smoking machine, and use a standard smoking with a suction volume of 35.0mL, a suction time of 2s, and a suction interval of 60s. Smoking rate for smoking cigarettes. After smoking, the smoke free radical solution was collected at a constant volume, and then frozen in liquid nitrogen. The assay volume was 200 μL.

By detecting the content of free radicals produced by cigarettes added with different contents and different ratios of natural antioxidant synergistic composition liquid proliposomes, compared with the control, the effect of the composition on gas phase free radicals in cigarette smoke is calculated. Clear effect. The experimental results are shown in Table 1:

Table 1 :

Experimental conclusion: From the above examples on the scavenging effect of cigarette gas phase free radicals, it can be seen that when the amount of natural antioxidants contained in the liquid proliposome is about 25-45%, it has a better scavenging effect on gas phase free radicals , especially when the proportioning ratio of squalene and tea polyphenols is between 1:8～8:1, especially between 2:7～7:2, the composition has obvious effect on gas phase free radical scavenging The effect is that its ability to scavenge free radicals is enhanced with the increase of the amount of antioxidants. According to actual production, squalene and tea polyphenols are contained in liquid proliposomes of the natural antioxidant composition used in cigarette filters at about 30% to 45%, and the ratio of squalene and tea polyphenols is The synergistic effect on the scavenging ability of gas phase free radicals is obvious between 2:7 and 7:2.

Claims (10)

1. a liquid proliposome containing squalene and tea polyphenol composition, is characterized in that, described liquid proliposome is prepared by each component of following mass parts: Hydrogenated phospholipids: 7 to 10 parts; Stabilizer: 8 to 12 parts; Surfactant: 10-15 parts; Composition of squalene and tea polyphenols: 25-45 parts; remaining water; The sum of the mass parts of the above-mentioned components is 100 parts; The hydrogenated phospholipid is one or both of hydrogenated lecithin or hydrogenated soybean lecithin; Described stabilizing agent is hydroxyl starch; The surfactant is glycerol. 2. the liquid proliposome containing squalene and tea polyphenol composition according to claim 1, is characterized in that, is prepared by each component of following mass parts: Hydrogenated phospholipids: 8-9 parts; Stabilizer: 9 to 11 parts; Surfactant: 12 to 14 parts; Composition of squalene and tea polyphenols: 30-35 parts; remaining water; The sum of the parts by mass of the above-mentioned components is 100 parts. 3. the liquid proliposome containing squalene and tea polyphenol composition according to claim 1, is characterized in that, described hydroxystarch is in hydroxymethyl starch, hydroxyethyl starch or hydroxypropyl starch one or more of . 4. the liquid proliposome containing squalene and tea polyphenols composition according to claim 1, is characterized in that, in described squalene and tea polyphenols composition, squalene and tea polyphenols The mass ratio is 1:8～8:1. 5. The application of the liquid proliposome containing squalene and tea polyphenol composition according to any one of claims 1 to 4, characterized in that, the liquid proliposome is applied to the hookah filter tip, and the liquid proliposome The plastid is packed into a hollow cylinder with waterproof, dustproof and air-permeable membranes at both ends, and is built into the cigarette filter tip of acetate fiber during the forming process of the cigarette filter rod. 6. The application according to claim 5, characterized in that 0.5-1 gram of the liquid proliposome containing squalene and tea polyphenols composition is built in each cigarette filter tip. 7. The method for preparing the liquid proliposome containing squalene and tea polyphenol composition according to any one of claims 1 to 4, characterized in that it comprises the following steps: S1. Dissolving the composition of phospholipids, squalene and tea polyphenols in glycerol to obtain a mixed system; S2. Homogenize the mixed system obtained in S1 at high speed to make it fully mixed to obtain a homogenized mixed system; S3. Mix the stabilizer and water to obtain an aqueous solution of the stabilizer, add the aqueous solution of the stabilizer to the homogenized mixing system obtained in S2 and stir evenly to form a stable particle suspension to obtain the composition containing squalene and tea polyphenols liquid proliposomes. 8. according to the preparation method of the liquid proliposome containing squalene and tea polyphenol composition described in claim 7, it is characterized in that, the process condition of high-speed homogenization described in S2 is: 2000 r/min, stirring 45min. 9. The liquid proliposome containing squalene and tea polyphenol composition that the method described in claim 7 prepares. 10. the application of the liquid proliposome containing squalene and tea polyphenol composition described in claim 9 is characterized in that, it is characterized in that, is applied to hookah type filter tip, is described liquid prolipid The body is packed into a hollow cylinder with waterproof, dustproof and air-permeable membranes at both ends, and is built into the cigarette filter tip of acetate fiber during the forming process of the cigarette filter tip rod.