CN103875448B - The cultural method of Ganoderma lucidum mycelium - Google Patents
The cultural method of Ganoderma lucidum mycelium Download PDFInfo
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- CN103875448B CN103875448B CN201410095636.9A CN201410095636A CN103875448B CN 103875448 B CN103875448 B CN 103875448B CN 201410095636 A CN201410095636 A CN 201410095636A CN 103875448 B CN103875448 B CN 103875448B
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- ganoderma lucidum
- millet
- rice
- corn
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 33
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 18
- 244000062793 Sorghum vulgare Species 0.000 claims abstract description 30
- 235000019713 millet Nutrition 0.000 claims abstract description 30
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 25
- 235000015097 nutrients Nutrition 0.000 claims abstract description 25
- 235000009566 rice Nutrition 0.000 claims abstract description 25
- 240000008042 Zea mays Species 0.000 claims abstract description 24
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 24
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 24
- 235000005822 corn Nutrition 0.000 claims abstract description 24
- 235000021307 Triticum Nutrition 0.000 claims abstract description 23
- 240000001417 Vigna umbellata Species 0.000 claims abstract description 23
- 235000011453 Vigna umbellata Nutrition 0.000 claims abstract description 23
- 240000004922 Vigna radiata Species 0.000 claims abstract description 22
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims abstract description 22
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims abstract description 22
- 238000000855 fermentation Methods 0.000 claims abstract description 16
- 230000004151 fermentation Effects 0.000 claims abstract description 16
- 241000222336 Ganoderma Species 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 5
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 239000012530 fluid Substances 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims abstract 8
- 241000209140 Triticum Species 0.000 claims description 22
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims 3
- 244000000231 Sesamum indicum Species 0.000 claims 1
- 235000003434 Sesamum indicum Nutrition 0.000 claims 1
- 244000013123 dwarf bean Species 0.000 claims 1
- 235000021331 green beans Nutrition 0.000 claims 1
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 231100000614 poison Toxicity 0.000 abstract description 3
- 230000007096 poisonous effect Effects 0.000 abstract description 3
- 244000098338 Triticum aestivum Species 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 17
- 235000013339 cereals Nutrition 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000007199 Panicum miliaceum Nutrition 0.000 description 1
- 244000115721 Pennisetum typhoides Species 0.000 description 1
- 235000007195 Pennisetum typhoides Nutrition 0.000 description 1
- 235000008515 Setaria glauca Nutrition 0.000 description 1
- 240000005498 Setaria italica Species 0.000 description 1
- 235000007226 Setaria italica Nutrition 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 244000022185 broomcorn panic Species 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the cultural method of Ganoderma lucidum mycelium, comprise the following steps：1）Configure nutrient solution and sterilized；2）To foregoing Solution cooling to less than 30 DEG C, then lucid ganoderma stock culture is inoculated with aseptic condition；3）The nutrient solution for being inoculated with lucid ganoderma stock culture is put into the rotatable fermentation tank that design temperature is 25 DEG C to 28 DEG C and stood；4）Fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium, the nutrient solution is natural nutrient fluid, formed by the configuration of mung bean, red bean, white sesameseed, corn, wheat, millet and rice, then the material of whole break into pulverizer to the powder of 300 500 mesh, then by the pulvis of mixing and water with（50‑70）：（30‑50）Proportional arrangement form.Cost of the present invention is low, and nutritive value is high, environment-protecting and non-poisonous harmless.
Description
Technical field
The present invention relates to Ganoderma lucidum mycelium culture technique field, specifically a kind of technology of the cultural method of Ganoderma lucidum mycelium.
Background technology
Ganoderma lucidum, there is preventing and curing diseases, promote longevity, wherein GL-B is that ganoderma lucidum strengthens the body resistance to consolidate the constitution, nourished by force
The main component strengthen, promoted longevity.Ganoderma lucidum, can be with medicine-food two-purpose due to having no toxic side effect.Nowadays Wild ganoderma resource is not yet
Disconnected reduction.At present, the ganoderma lucidum product of in the market uses artificial cultivation mostly, but artificial cultivation is due to agricultural chemicals to be used and chemistry
Method cultivates strain, agricultural chemicals is used during cultivation management to control germ insect pest, therefore bring chemistry to ganoderma lucidum fruitbody
Pollution.And the biological transformation ratio of ganoderma lucidum is all very low always, the cycle length of Ganoderma Lucidum, plus production technology and managed
Journey it is complicated and it is cumbersome make production cost cross height cause inefficiencies.On the other hand, the various cultures that people have carried out Ganoderma lucidum mycelium are ground
Study carefully.
The content of the invention
It is an object of the invention to provide a kind of cost is low, nutritive value is high, the culture of environment-protecting and non-poisonous harmless Ganoderma lucidum mycelium
Method.
Realized below by following technical scheme：
A kind of cultural method of Ganoderma lucidum mycelium, it is characterised in that the cultural method of the Ganoderma lucidum mycelium comprises the following steps：
1）Configure nutrient solution and sterilized；
2）To foregoing Solution cooling to less than 30 DEG C, then lucid ganoderma stock culture is inoculated with aseptic condition；
3）The nutrient solution for being inoculated with lucid ganoderma stock culture is put into the rotatable fermentation tank that design temperature is 25 DEG C to 28 DEG C
Stand；
4）Fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium.
The nutrient solution is natural nutrient fluid, by the configuration of mung bean, red bean, white sesameseed, corn, wheat, millet and rice
Into, then the material of whole breaks into pulverizer to the powder of 300-500 mesh, then by the pulvis of mixing and water with（50-70）：
（30-50）Proportional arrangement form.
The step 3）Middle time of repose at least 30 hours, step 4）In should make the nutrient solution and air of inoculation lucid ganoderma stock culture
Contact is abundant.
The mung bean, red bean, white sesameseed, corn, wheat, millet and rice are configured by following parts by weight：Mung bean 8-12,
Red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16.
Specific proportioning has following scheme：
13 parts of 8 parts of the mung bean, 5 parts of red bean, 3 parts of white sesameseed, 23 parts of corn, 23 parts of wheat, 13 parts of millet and rice.
16 parts of 12 parts of the mung bean, 7 parts of red bean, 4 parts of white sesameseed, 27 parts of corn, 27 parts of wheat, 16 parts of millet and rice.
15 parts of 8 parts of the mung bean, 5 parts of red bean, 3 parts of white sesameseed, 26 parts of corn, 26 parts of wheat, 15 parts of millet and rice.
13 parts of 9 parts of the mung bean, 6 parts of red bean, 3 parts of white sesameseed, 26 parts of corn, 26 parts of wheat, 16 parts of millet and rice.
The present invention has advantages below compared with prior art.
The present invention is due to the natural nutrition formed using mung bean, red bean, white sesameseed, corn, wheat, millet and rice configuration
Liquid is as culture medium so that the nutrient source of the Ganoderma lucidum mycelium of culture is more abundant with, being advantageous to mycelia absorption and breeding comprehensively, keeps away
Exempt to carry out cultivating caused defect using single corn.The present invention does not have source of heavy metal pollution, and cost is low, nutritive value
Height, it is environment-protecting and non-poisonous harmless.Fermented and cultured is rotated especially with fermentation tank, sterile oxygen is fully utilized, and is connected to ganoderma lucidum mother
The nutrient solution of kind is more abundant with air contact, substantially increases the biological transformation ratio of ganoderma lucidum, shortens cultivation cycle, makes production work
Skill and management process become simple.The configuration raw material of nutrient solution of the present invention is extensive, and cost is low, is advantageous to the popularization of technology, and
With the ganoderma lucidum natural health of this natural grain culture.
Embodiment
The present invention is described in further detail below.
The cultural method of Ganoderma lucidum mycelium of the present invention, comprises the following steps：
1）Configure nutrient solution and sterilized；
2）To foregoing Solution cooling to less than 30 DEG C, then lucid ganoderma stock culture is inoculated with aseptic condition；
3）The nutrient solution for being inoculated with lucid ganoderma stock culture is put into the rotatable fermentation tank that design temperature is 25 DEG C to 28 DEG C
Stand；
4）Fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium.
Abovementioned steps 3）Middle time of repose at least 30 hours, step 4）In should make the nutrient solution and air of inoculation lucid ganoderma stock culture
Contact is abundant.Wherein nutrient solution is natural nutrient fluid, by the configuration of mung bean, red bean, white sesameseed, corn, wheat, millet and rice
Into, then the material of whole breaks into pulverizer to the powder of 300-500 mesh, then by the pulvis of mixing and water with（50-70）：
（30-50）Proportional arrangement form.Wherein rice can be thick rice.Millet in the present invention, its scientific name：Setaria
Italica, China is ancient to claim millet or grain.Grain made of shelling, because it is small, the millimeter of diameter 2 is therefore named.Originate in China
The northern Huanghe valley, the staple food crop of ancient Chinese, so Xia dynasty and Shang dynasty belong to " grain culture ".Grain grows drought-enduring, product
Kind is various, is commonly called as " grain has the five colours ", the millet for having white, red, yellow, black, orange, purple a variety of colors, also toughness millet.It is Chinese earliest
Wine be also to be brewageed with millet.Grain is adapted to the area growth for lacking irrigation in arid.Its stem, leaf are harder, can raise
Material, typically only ox can digest.Grain is commonly called as millet in north of China.Western language is typically to grain, broomcorn millet, cattailmillet and some other grain
Small coarse cereals have general designation, and non-agricultural expert is typically regardless of as English claims " millet ".
The present invention describes in more detail：Preparing natural nutrient solution first, then sterilized under 1.5 kilograms of pressure 2 hours with
On, less than 30 degree are cooled to when reaching sterility requirements, then inoculation method conventional under aseptic condition access lucid ganoderma stock culture.It will connect
It is in 25 to the 28 rotatable fermentation tanks spent that kind, which has the design temperature that is put into of the nutrient solution of parent species, stands 30 hours after fermentation tanks
Start slowly to rotate so that object exchanges and adds oxygen up and down in tank, sterile oxygen is fully utilized during rotation, connects
Have that nutrient solution and the air contact of female bacterium are more abundant, so fermentation 7 to ten days, a large amount of ripe Ganoderma lucidum myceliums are had in fermentation tank.
The new fresh thalli of harvest can be used for producing after centrifuge is overanxious.
Mung bean, red bean, white sesameseed, corn, wheat, millet and rice are configured by following parts by weight in the present invention：Mung bean
8-12, red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16.
Specific proportioning has following scheme：Can by kilogram in units of configured.Specifically how much matched somebody with somebody according to aequum
Put.
13 parts of 8 parts of mung bean, 5 parts of red bean, 3 parts of white sesameseed, 23 parts of corn, 23 parts of wheat, 13 parts of millet and rice；
16 parts of 12 parts of mung bean, 7 parts of red bean, 4 parts of white sesameseed, 27 parts of corn, 27 parts of wheat, 16 parts of millet and rice；
15 parts of 8 parts of mung bean, 5 parts of red bean, 3 parts of white sesameseed, 26 parts of corn, 26 parts of wheat, 15 parts of millet and rice；
13 parts of 9 parts of mung bean, 6 parts of red bean, 3 parts of white sesameseed, 26 parts of corn, 26 parts of wheat, 16 parts of millet and rice.
Claims (6)
1. a kind of cultural method of Ganoderma lucidum mycelium, it is characterised in that the cultural method of the Ganoderma lucidum mycelium comprises the following steps：
1) configure nutrient solution and sterilized；
2) then lucid ganoderma stock culture is inoculated with aseptic condition to 30 DEG C below to foregoing Solution cooling；
3) nutrient solution for being inoculated with lucid ganoderma stock culture is put into the rotatable fermentation tank that design temperature is 25 DEG C to 28 DEG C and carried out
Stand；
4) the rotatable fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium；
The nutrient solution is natural nutrient fluid, is formed by the configuration of mung bean, red bean, white sesameseed, corn, wheat, millet and rice, so
The material of whole is broken into the powder of 300-500 mesh with pulverizer afterwards, then by the pulvis of mixing and water with (50-70)：(30-50)
Proportional arrangement form, the mung bean, red bean, white sesameseed, corn, wheat, millet and rice by following parts by weight configure：It is green
Beans 8-12, red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16,
It should make that the nutrient solution of inoculation lucid ganoderma stock culture and air contact are abundant, and its measure is stood by step 3) in step 4)
The rotatable fermentation tank just starts slowly to rotate so that object exchanges increase oxygen again, so fermentation up and down in tank afterwards.
2. the cultural method of Ganoderma lucidum mycelium according to claim 1, it is characterised in that：Time of repose is at least in the step 3)
30 hours, such fermentation time was seven to ten days in step 4).
3. the cultural method of Ganoderma lucidum mycelium according to claim 1, it is characterised in that：8 parts of the mung bean, 5 parts of red bean, Bai Zhi
13 parts of 3 parts of fiber crops, 23 parts of corn, 23 parts of wheat, 13 parts of millet and rice.
4. the cultural method of Ganoderma lucidum mycelium according to claim 1, it is characterised in that：It is 12 parts of the mung bean, 7 parts of red bean, white
16 parts of 4 parts of sesame, 27 parts of corn, 27 parts of wheat, 16 parts of millet and rice.
5. the cultural method of Ganoderma lucidum mycelium according to claim 1, it is characterised in that：8 parts of the mung bean, 5 parts of red bean, Bai Zhi
15 parts of 3 parts of fiber crops, 26 parts of corn, 26 parts of wheat, 15 parts of millet and rice.
6. the cultural method of Ganoderma lucidum mycelium according to claim 1, it is characterised in that：9 parts of the mung bean, 6 parts of red bean, Bai Zhi
13 parts of 3 parts of fiber crops, 26 parts of corn, 26 parts of wheat, 16 parts of millet and rice.
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| CN201410095636.9A CN103875448B (en) | 2014-03-17 | 2014-03-17 | The cultural method of Ganoderma lucidum mycelium |
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| CN103875448B true CN103875448B (en) | 2017-12-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105309197A (en) * | 2014-07-26 | 2016-02-10 | 雷色香 | Preparation method of lucid ganoderma rice |
| CN104957514A (en) * | 2015-07-09 | 2015-10-07 | 淮阴师范学院 | Method for preparing lucid ganoderma rice meal by utilizing ganoderma lucidum mycelia to ferment rice |
| CN107047061B (en) * | 2017-04-21 | 2020-10-27 | 广西壮族自治区农业科学院微生物研究所 | Efficient method for cultivating lucid ganoderma and culture medium thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059457A (en) * | 1991-10-22 | 1992-03-18 | 北京市营养源研究所 | The manufacture method of nutriment from " Xianggu mushroom |
| CN1105208A (en) * | 1994-09-28 | 1995-07-19 | 湖南省益阳伊嘉生物工程公司 | Health beverage of glossy ganoderma series |
| CN1188801A (en) * | 1998-01-14 | 1998-07-29 | 李宝健 | Ganoderma lucidum 10 ton tank fermentation technology |
| CN101875588A (en) * | 2009-04-30 | 2010-11-03 | 吴继高 | Glossy ganoderma culture material |
| CN103300283A (en) * | 2012-03-15 | 2013-09-18 | 李声涛 | Production technique of biological fungi food |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002176968A (en) * | 2000-10-06 | 2002-06-25 | Kabaiotekku Co Ltd | Process for cultivating mycelium of mushrooms using grains, products and uses thereof |
| KR100380945B1 (en) * | 2000-10-06 | 2003-04-26 | 송재만 | Process for mycelial culture using washing water of the grain and product thereof |
-
2014
- 2014-03-17 CN CN201410095636.9A patent/CN103875448B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059457A (en) * | 1991-10-22 | 1992-03-18 | 北京市营养源研究所 | The manufacture method of nutriment from " Xianggu mushroom |
| CN1105208A (en) * | 1994-09-28 | 1995-07-19 | 湖南省益阳伊嘉生物工程公司 | Health beverage of glossy ganoderma series |
| CN1188801A (en) * | 1998-01-14 | 1998-07-29 | 李宝健 | Ganoderma lucidum 10 ton tank fermentation technology |
| CN101875588A (en) * | 2009-04-30 | 2010-11-03 | 吴继高 | Glossy ganoderma culture material |
| CN103300283A (en) * | 2012-03-15 | 2013-09-18 | 李声涛 | Production technique of biological fungi food |
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