CN114149919A

CN114149919A - A kind of cultivation device for cassava genetic information transformation

Info

Publication number: CN114149919A
Application number: CN202111461752.4A
Authority: CN
Inventors: 王力敏; 张洪霞; 李蓓; 赵立子; 孙焕喜; 房凤艳; 郑松峰; 宋炫晨
Original assignee: Ludong University
Current assignee: Ludong University
Priority date: 2021-12-02
Filing date: 2021-12-02
Publication date: 2022-03-08
Anticipated expiration: 2041-12-02
Also published as: CN114149919B

Abstract

The invention discloses a culture device for cassava genetic information transformation, which comprises: the box body is provided with a first motor on the outer surface of a left panel of the box body; the U-shaped plate is connected with the first motor through a rotating shaft and is connected with the loading frame through an upright post; the loading frame is connected with mounting seats at two ends; the gear is sleeved on the transmission shaft and meshed with the outer teeth, and one end of the transmission shaft is connected with the second motor; the sliding block is arranged on the sliding rail, and a side plate is fixed on the top surface of the sliding block; the culture bottle, the bottleneck of culture bottle stretches into the mount pad. This a culture apparatus for cassava genetic information conversion utilizes two blake bottles about the mount pad intercommunication, makes the position of two blake bottles reverse through the upset load carrier, and the liquid in the upside blake bottle flows into the downside blake bottle, conveniently changes the solution that soaks the cassava, avoids taking out the cassava of blake bottle, reduces the cassava and receives external disturbance's risk.

Description

A culture apparatus for cassava genetic information conversion

Technical Field

The invention relates to the related technical field of genetic engineering, in particular to a culture device for cassava genetic information transformation.

Background

Cassava is a plant of the genus cassava of the family euphorbiaceae, is drought-tolerant and barren-resistant, is one of three tuber crops, is the sixth grain crop in the world, is called the king of starch, and is a grain for nearly six hundred million people in the world.

The conventional cassava genetic information transformation is to infect cassava callus by agrobacterium, the cassava callus needs to be added into an invasive dye solution to be soaked and shaken for 20 minutes in the process, then the cassava callus is taken out to be cultured in a dark room for 48 hours, then the cassava callus is added into sterile water to be subjected to sterilization treatment, and finally the cassava callus is added into a screening culture medium to be cultured in the dark room for one month.

Disclosure of Invention

The invention provides a culture device for cassava genetic information transformation in order to make up for market blank.

The invention aims to provide a culture device for cassava genetic information transformation, which solves the problems that the time required by cassava genetic transformation is increased and the risk that the cassava callus is interfered by external bacteria is increased when the cassava callus needs to be transferred back and forth in the prior art proposed in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: a culture device for cassava genetic information transformation, comprising:

the motor-driven box comprises a box body, wherein a first motor is installed on the outer surface of a left panel of the box body, and a sliding rail is arranged on the inner wall of a bottom plate of the box body;

the U-shaped plate is connected with the first motor through a rotating shaft and is connected with the loading frame through an upright post;

the device comprises a loading frame, wherein two ends of the loading frame are both connected with mounting seats, heating rings, partition plates and grid rods are mounted in the mounting seats, and screw holes are formed in the upper end and the lower end of each mounting seat;

the gear is sleeved on the transmission shaft and meshed with the outer teeth, and one end of the transmission shaft is connected with the second motor;

the sliding block is arranged on the sliding rail, a side plate is fixed on the top surface of the sliding block, and a heating wire and a lamp bead are embedded in the side plate;

the culture bottle, the bottleneck of culture bottle stretches into the mount pad, install the breather plug on the lateral wall of culture bottle.

Further, the pivot runs through the left panel of box, the both ends of pivot are connected respectively the U-shaped board with first motor, first motor is used for the drive the U-shaped board rotates, the interval between two boards about the U-shaped board is greater than the thickness of loading frame.

Further, the bottom mounting of stand is in on the bottom plate of U-shaped board, the stand is worn out the roof of U-shaped board is connected the roof, the roof with the transmission shaft links to each other, the bottom of transmission shaft runs through gear connection the second motor, the second motor is fixed on the bottom plate of U-shaped board, the second motor is used for the drive gear revolve.

Furthermore, the outer teeth are arranged on the outer arc surface of the loading frame, a convex block is arranged in the middle of the inner arc surface of the loading frame, a jack is formed in the convex block, and the upright post is inserted in the jack.

Furthermore, a partition plate is horizontally fixed in the middle of the inner wall of the mounting seat, the grid rods are installed at the circle centers of the partition plate, the upper ends and the lower ends of the grid rods respectively extend into the corresponding screw holes, and heating rings are symmetrically arranged in the mounting seat on the upper side and the lower side of the partition plate.

Furthermore, the radius of the inner ring of the heating ring is larger than that of the mounting seat, an electrode column is installed on the heating ring and extends into the limiting groove, an electrode plate is installed in the limiting groove, and the electrode column is matched with the electrode plate.

Furthermore, the bottom of the slider is provided with a sliding groove, a through hole is formed in a top plate of the slider, a fan is installed in the through hole, the slider is symmetrically provided with the side plates on the front side and the rear side of the through hole, and the distance between the opposite surfaces of the side plates is larger than the diameter of the culture bottle.

Further, the heater strip is used for providing and cultivates the temperature, the lamp pearl is used for providing and cultivates the illumination, the heater strip with lamp pearl evenly distributed is in on the curb plate.

Furthermore, the vent plug is close to the bottle mouth, and the bottle mouth is matched with the screw hole.

Compared with the prior art, the invention has the beneficial effects that: the culture device for cassava genetic information conversion is reasonable in structure arrangement, the upper culture bottle and the lower culture bottle are communicated by the mounting seat, the positions of the two culture bottles are reversed by turning over the loading frame, liquid in the upper culture bottle flows into the lower culture bottle, cassava soaking solution is convenient to replace, cassava in the culture bottles is prevented from being taken out, the risk that the cassava is interfered by the outside is reduced, the bottle openings of the culture bottles loaded each time are disinfected by the heating rings, the burden of operators is reduced, the side plates are driven by the sliding blocks to move, and a proper culture environment is provided for the culture bottles;

1. the mounting seat is provided with screw holes which are communicated up and down, so that two culture bottles on the same mounting seat can be conveniently communicated with each other, and the mounting seat is internally provided with a partition plate and a grid rod, so that liquid in the culture bottles can be transferred through the mounting seat, and solid is retained in the culture bottles, thereby reducing the exposure times of cassava;

2. the gear is meshed with the outer teeth, and the second motor drives the gear to rotate so that the loading frame shakes back and forth, so that a shaking effect is realized, and the transfer of the culture bottles to shaking table equipment is avoided;

3. the side plate and the fan on the sliding block move along the sliding rail along with the sliding block, so that the side plate and the fan move to the periphery of the culture bottle, the temperature and the illumination condition around the culture bottle are adjusted, and the culture bottle is prevented from being transferred to darkroom equipment;

4. the upper and lower both sides at the baffle are provided with the heating ring, and when not installing the blake bottle in the downside screw, the heating ring of downside leaves the baffle under the action of gravity, and electrode post on the heating ring gos deep into spacing groove connection electrode piece, and the heating ring switches on the power and heaies up, packs the screw into the bottleneck this moment, and the sterilizing effect is realized to the heating ring of bottleneck contact high temperature, and the bottleneck upwards jack-up heating ring makes electrode post and electrode piece separation, accomplishes the outage effect simultaneously.

Drawings

FIG. 1 is a schematic top cross-sectional view of a structure of the present invention;

FIG. 2 is a schematic side sectional view of the structure of the present invention;

FIG. 3 is an enlarged cross-sectional view taken at A in FIG. 2 of the structure of the present invention;

FIG. 4 is a schematic sectional elevation view of the structure of the present invention;

FIG. 5 is a schematic view of a carrier of the present invention;

FIG. 6 is a schematic view of a slider of the present invention;

FIG. 7 is a schematic view of a heating ring of the present invention construction.

In the figure: 1. a box body; 2. a U-shaped plate; 3. a loading frame; 4. a gear; 5. a slider; 6. a culture bottle; 21. A rotating shaft; 22. a first motor; 23. a column; 31. an outer tooth; 32. a mounting seat; 33. a bump; 331. A jack; 321. a screw hole; 322. a heating ring; 323. a partition plate; 324. a grid rod; 325. a limiting groove; 326. an electrode sheet; 327. an electrode column; 41. a top plate; 42. a second motor; 43. a drive shaft; 51. A side plate; 52. a fan; 53. a slide rail; 54. a through hole; 55. heating wires; 56. a lamp bead; 57. a chute; 61. a vent plug; 62. and (5) opening the bottle.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The first embodiment is as follows: referring to fig. 1-7, the present invention provides a technical solution: a culture device for cassava genetic information transformation, comprising:

the motor-driven box comprises a box body 1, wherein a first motor 22 is arranged on the outer surface of a left panel of the box body 1, and a sliding rail 53 is arranged on the inner wall of a bottom plate of the box body 1;

the U-shaped plate 2 is connected with a first motor 22 through a rotating shaft 21, and the U-shaped plate 2 is connected with the loading frame 3 through an upright post 23;

the loading frame 3 is characterized in that two ends of the loading frame 3 are connected with mounting seats 32, heating rings 322, partition plates 323 and grid rods 324 are mounted in the mounting seats 32, and screw holes 321 are formed in the upper end and the lower end of each mounting seat 32;

the gear 4 is sleeved on the transmission shaft 43, the gear 4 is meshed with the external teeth 31, and one end of the transmission shaft 43 is connected with the second motor 42;

the sliding block 5 is arranged on the sliding rail 53, a side plate 51 is fixed on the top surface of the sliding block 5, and a heating wire 55 and a lamp bead 56 are embedded in the side plate 51;

the culture bottle 6, the bottle mouth 62 of the culture bottle 6 extends into the mounting seat 32, and the side wall of the culture bottle 6 is provided with a vent plug 61.

When the culture device for cassava genetic information transformation is used, cassava callus is added into a culture bottle 6, a box body 1 is opened, a bottle opening 62 of the culture bottle 6 is in threaded connection with a mounting seat 32, a first motor 22 is started to drive a rotating shaft 21 to rotate, a U-shaped plate 2 and a loading frame 3 are turned over for 180 degrees, the culture bottle 6 filled with the infection liquid is in threaded connection with the same mounting seat 32, the U-shaped plate 2 and the loading frame 3 are turned over again, the infection liquid flows into the cassava culture bottle 6 through a grid rod 324, a second motor 42 is started to drive a transmission shaft 43 to rotate, the transmission shaft 43 drives a gear 4 to rotate, the gear 4 drives the loading frame 3 to rotate around an upright post 23, the effect of shaking the culture bottle 6 is realized, after infection, the loading frame 3 is turned over, the infection liquid is separated from cassava, the culture bottle 6 with the infection liquid is taken down, the culture bottle 6 with sterile water is loaded to turn over after a period of culture, and (3) allowing sterile water to enter the cassava culture bottle 6, repeating the operation, performing sterilization treatment, taking down the sterile water culture bottle 6, then loading the culture bottle 6 with culture solution, allowing the culture solution to enter the cassava culture bottle 6, and moving the sliding block 5 along the sliding rail 53 to enable the cassava culture bottle 6 to be in a proper culture environment.

The second embodiment is as follows: the present embodiment is further limited by the first embodiment, as shown in fig. 1 and 4, the rotating shaft 21 penetrates through the left panel of the box body 1, two ends of the rotating shaft 21 are respectively connected to the U-shaped plate 2 and the first motor 22, the first motor 22 is used for driving the U-shaped plate 2 to rotate, the distance between the upper and lower two plates of the U-shaped plate 2 is greater than the thickness of the loading frame 3, the U-shaped plate 2 is hinged to the loading frame 3, and when the first motor 22 drives the U-shaped plate 2 to turn, the loading frame 3 also turns.

The third concrete implementation mode: as shown in fig. 1, 4 and 5, the bottom end of the upright 23 is fixed on the bottom plate of the U-shaped plate 2, the upright 23 penetrates through the top plate 41 of the U-shaped plate 2 to connect with the top plate 41, the top plate 41 is connected with the transmission shaft 43, the bottom end of the transmission shaft 43 penetrates through the gear 4 to connect with the second motor 42, the second motor 42 is fixed on the bottom plate of the U-shaped plate 2, the second motor 42 is used for driving the gear 4 to rotate, when the second motor 42 drives the gear 4 to rotate, the loading frame 3 rotates around the upright 23, the shaking effect is achieved, and the substances in the culture bottles 6 can be mixed uniformly.

The fourth concrete implementation mode: as shown in fig. 1 and 5, the outer arc surface of the loading frame 3 is provided with outer teeth 31, the middle part of the inner arc surface of the loading frame 3 is provided with a convex block 33, the convex block 33 is provided with a jack 331, and the upright column 23 is inserted into the jack 331.

The fifth concrete implementation mode: the embodiment is further limited by the first specific embodiment, as shown in fig. 2 and 3, a partition 323 is horizontally fixed in the middle of the inner wall of the mounting seat 32, a grid rod 324 is installed at the center of the circle of the partition 323, the upper end and the lower end of the grid rod 324 respectively extend into the corresponding screw holes 321, heating rings 322 are symmetrically arranged in the mounting seat 32 at the upper side and the lower side of the partition 323, and the grid rod 324 not only has a filtering effect, but also can accelerate the liquid flow, and has a guiding function to prevent the liquid from flowing away.

The sixth specific implementation mode: the fifth embodiment is further limited by the fifth embodiment, the radius of the inner ring of the heating ring 322 is larger than the radius of the mounting seat 32, the electrode post 327 is mounted on the heating ring 322, the electrode post 327 extends into the limiting groove 325, the electrode plate 326 is mounted in the limiting groove 325, the electrode post 327 is matched with the electrode plate 326, the heating ring 322 falls downward under the action of gravity, the electrode post 327 on the heating ring 322 is in contact with the electrode plate 326, so that the heating ring 322 is electrified for sterilization, when the flask mouth 62 jacks the heating ring 322 when the culture flask 6 is mounted, the electrode post 327 is separated from the electrode plate 326, and the heating ring 322 stops heating.

The seventh embodiment: this embodiment is the further restriction of embodiment one, and spout 57 has been seted up to the bottom of slider 5, has seted up through-hole 54 on the roof 41 of slider 5, installs fan 52 in the through-hole 54, and the symmetry is installed side plate 51 on the slider 5 of both sides around the through-hole 54, and the interval between the opposite face of side plate 51 is greater than the diameter of blake bottle 6, sets up slider 5 and slide rail 53 and avoids sheltering from the upset of blake bottle 6, only moves around blake bottle 6 when needs.

The specific implementation mode is eight: the embodiment is further limited by the first specific embodiment, as shown in fig. 1 and 6, the heating wire 55 is used for providing the culture temperature, the lamp beads 56 are used for providing the culture illumination, the heating wire 55 and the lamp beads 56 are uniformly distributed on the side plate 51, the requirements on the environment in different culture stages are different, and the environmental conditions are flexibly adjusted through the heating wire 55 and the lamp beads 56, so that the growth requirements of cassava are met.

The specific implementation method nine: as shown in fig. 2 and 3, the vent plug 61 is close to the bottle opening 62, the bottle opening 62 is matched with the screw hole 321, the vent plug 61 is convenient to release gas or provide oxygen according to requirements during the culture process, a suitable culture environment is constructed, and the screw hole 321 is provided to facilitate the assembly and disassembly of the culture bottle 6.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. a culture device for cassava genetic information transformation, is characterized in that, comprises:

A box body (1), a first motor (22) is installed on the outer surface of the left panel of the box body (1), and a slide rail (53) is arranged on the inner wall of the bottom plate of the box body (1);

U-shaped plate (2), the U-shaped plate (2) is connected to the first motor (22) through a rotating shaft (21), and the U-shaped plate (2) is connected to the loading frame (3) through a column (23);

A loading rack (3), both ends of the loading rack (3) are connected with mounting seats (32), and a heating ring (322), a partition plate (323) and a grid rod are installed in the mounting seat (32) (324), the upper and lower ends of the mounting seat (32) are provided with screw holes (321);

A gear (4) is sleeved on the transmission shaft (43), the gear (4) is meshed with the external teeth (31), and one end of the transmission shaft (43) is connected to the second motor (42);

The sliding block (5) is mounted on the sliding rail (53), a side plate (51) is fixed on the top surface of the sliding block (5), and a heating wire (51) is embedded on the side plate (51). 55) and lamp beads (56);

A culture bottle (6), the bottle mouth (62) of the culture bottle (6) extends into the mounting seat (32), and a vent plug (61) is installed on the side wall of the culture bottle (6).

2. a kind of cultivating device for cassava genetic information transformation according to claim 1, is characterized in that: described rotating shaft (21) runs through the left panel of described box (1), and described rotating shaft (21) Both ends are respectively connected to the U-shaped plate (2) and the first motor (22), the first motor (22) is used to drive the U-shaped plate (2) to rotate, and the U-shaped plate (2) ) The distance between the upper and lower plates is greater than the thickness of the loading frame (3).

3. a kind of cultivation device for cassava genetic information transformation according to claim 1, is characterized in that: the bottom end of described upright (23) is fixed on the bottom plate of described U-shaped plate (2), described The upright column (23) penetrates through the top plate of the U-shaped plate (2) to connect to the top plate (41), the top plate (41) is connected with the transmission shaft (43), and the bottom end of the transmission shaft (43) penetrates through the top plate (41). The gear (4) is connected to the second motor (42), the second motor (42) is fixed on the bottom plate of the U-shaped plate (2), and the second motor (42) is used to drive the Gear (4) turns.

4. a kind of cultivation device for cassava genetic information transformation according to claim 2, is characterized in that: the outer arc surface of described loading rack (3) is provided with described outer tooth (31), described loading A convex block (33) is provided in the middle of the inner arc surface of the frame (3), an insertion hole (331) is opened on the convex block (33), and the upright column (23) is inserted into the insertion hole (331). .

5. a kind of cultivating device for cassava genetic information transformation according to claim 1, is characterized in that: the inner wall middle part of described mounting seat (32) is horizontally fixed with dividing plate (323), described dividing plate (323) The grid rod (324) is installed at the center of the circle of the A heating ring (322) is symmetrically arranged inside the mounting seat (32) on the side.

6. a kind of cultivation device for cassava genetic information transformation according to claim 5 is characterized in that: the inner ring radius of described heating ring (322) is greater than the radius of described mounting seat (32), and described heating An electrode column (327) is installed on the ring (322), the electrode column (327) extends into the limiting groove (325), and an electrode sheet (326) is installed in the limiting groove (325), and the The electrode column (327) is adapted to the electrode sheet (326).

7. a kind of cultivating device for cassava genetic information transformation according to claim 1, is characterized in that: the bottom of described slider (5) is provided with chute (57), and the bottom of described slider (5) A through hole (54) is opened on the top plate, a fan (52) is installed in the through hole (54), and the sliding block (5) on the front and rear sides of the through hole (54) is symmetrically installed with the side The plate (51), the distance between the opposite surfaces of the side plate (51) is larger than the diameter of the culture flask (6).

8. a kind of cultivation device for cassava genetic information transformation according to claim 1, is characterized in that: described heating wire (55) is used for providing cultivation temperature, and described lamp bead (56) is used for providing cultivation illumination , the heating wire (55) and the lamp beads (56) are evenly distributed on the side plate (51).

9. a kind of cultivation device for cassava genetic information transformation according to claim 1, is characterized in that: described vent plug (61) is close to described bottle mouth (62), described bottle mouth (62) and all The screw holes (321) are suitable.