CN114350487A - A manipulator for single-cell sampling and quick needle change - Google Patents

Abstract

The invention provides an operating instrument for single cell sampling and quick needle replacement. The invention includes a workbench, a support structure and a single-cell sampling device fixed on the support structure, the single-cell sampling device and the support structure have a downwardly inclined angle of 45°, and the single-cell sampling device includes a moving slide rail and a sliding A moving sample feeding mechanism connected to the moving slide rail, the moving sample feeding mechanism includes a moving slide block and a hollow connecting rod fixedly connected above it, the front end of the hollow connecting rod is detachably connected by a clip needle locking member There is a sample injection needle, and the two ends of the moving slide rail are provided with limit blocks for restricting the displacement of the mobile sample introduction mechanism. The limit block located at the bottom is the sampling state positioning block, and the limit block located above is the sampling state. positioning block. The single-cell sampling needle tip of the invention is convenient to replace, the sampling probe can be quickly replaced after one sampling, and at the same time, the slider is moved to lock and fix at an arbitrary position, which is helpful for single-cell high-throughput detection.

Description

A manipulator for single-cell sampling and quick needle change

technical field

The invention relates to the technical field of single-cell sampling, in particular to an operating instrument for single-cell sampling and rapid needle replacement.

Background technique

Single-cell analysis technology is a technology that detects trace metabolites in a single cell by means of high sensitivity and high specificity. Existing single-cell sampling methods applied to fresh animal and plant tissues mainly absorb cell solutions by inserting extremely thin capillary probes into single cells. For example, "A Single Cell Sampling Device and Sampling Method Based on Porous Metal Enrichment Probes" disclosed in the Bulletin No. CN105713829B, which consists of a base, a support rod, a clamping device, a micrometer head and a fixed seat, and the sampling method is simple and fast. ,cheap. Announcement No. CN105575752B, "A Dual Function Equal Inner Diameter Skin Flow Mass Spectrometer Spray Needle and Preparation Method", discloses the preparation method of the mass spectrometer spray needle, and also discloses the sampling method of the mass spectrometer spray needle. The prior art lacks high-precision control of the position of the sampling needle tip, lacks operability in the process of replacing the ionization needle, and only provides a method for applying the position of the nanoliter electrospray needle, which is inconvenient for the repeated replacement of the nanoliter spray needle. Replacement is not easy enough. In this regard, it is not conducive to the rapid and high-throughput detection of single cells.

SUMMARY OF THE INVENTION

According to the technical problems raised above, an operating instrument for single-cell sampling and rapid needle replacement is provided. The technical means adopted in the present invention are as follows:

An operating instrument for single-cell sampling and rapid replacement of spray needles, comprising a workbench, a support structure and a single-cell sampling device fixed on the support structure, the single-cell sampling device and the support structure have a downwardly inclined angle, The single-cell sampling device comprises a moving slide rail and a moving sample feeding mechanism slidably connected to the moving slide rail, the moving sample feeding mechanism comprises a moving slide block and a hollow connecting rod fixedly connected above it, the hollow connecting rod A sample injection needle is detachably connected to the front end of the sliding rail through a clip needle locking member, and the two ends of the moving slide rail are provided with limit blocks for restricting the displacement of the mobile sample introduction mechanism, and the limit blocks located below are positioned in the sampling state. The upper limit block is the injection state positioning block.

Further, the support structure is a three-dimensional adjustment frame, and the bottom of the three-dimensional adjustment frame is connected to the bottom support connecting plate through the bottom support connecting rod.

Further, the hollow connecting rod is fixed on the bottom end fixing member of the hollow connecting rod through the front end locking member of the hollow connecting rod and the rear end locking member of the hollow connecting rod, and the bottom end fixing member of the hollow connecting rod is provided with on the mobile slider.

Further, one side wall of the moving slider is provided with a hole, and the slider stop locking member is screwed on the hole, which is used to fix the moving slider at the preset position of the slide rail.

In the present invention, a manual or electronically controlled three-dimensional adjustment frame is used to facilitate the rapid movement of the sampling needle after sampling to a preset position, and the sampling needle tip is connected by a device with rapid extraction and self-locking locking, which can realize the detection of single cells. The rapid replacement of the sampling needle tip enables efficient ionization of single-cell samples and facilitates single-cell high-throughput detection. At the same time, during the moving process of the moving slide rail with the slider, when the needle moves to a specific position, if you want to keep the needle tip in position, you can rotate the slider to stop the locking piece to fix the slider in a specific position, and you can replace it at this time. Sampling needle, easy to operate.

Description of drawings

In order to illustrate the embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description These are some embodiments of the present invention, and for those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.

FIG. 1 is a schematic diagram of the main structure of the present invention.

Figure 2 is a front view of the present invention in the state of replacing the needle.

Fig. 3 is a front view of the cell sampling state of the present invention.

Figure 4 is a side view of the present invention.

In the figure: 1. Bottom support connecting plate; 2. Bottom support connecting rod; 3. X-axis displacement platform connecting piece; 11. X-axis displacement rail; 12, Y-axis transmission motor; 13, Y-axis displacement rail; 14 , Y-axis moving slide rail; 15. Y-axis and Z-axis connecting support plate; 16. Z-axis displacement rail; 17. X-axis moving slide rail; 18. Z-axis transmission motor; 21. Single cell sampling device; 22. Move the sampling mechanism; 23. The Z-axis moves the slide rail.

Detailed ways

In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments These are some embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

As shown in Figures 1 to 4, the present invention discloses an operating instrument for single-cell sampling and rapid needle replacement, including a workbench, a support structure, and a single-cell sampling device 21 fixed on the support structure. The cell sampling device and the support structure have a downward inclination angle, which is 45 degrees in this embodiment. The single-cell sampling device includes a Z-axis moving slide rail and a mobile sampling mechanism slidably connected to the Z-axis moving slide rail 23 22. The moving sample feeding mechanism includes a moving slider and a hollow connecting rod fixedly connected above it, and the front end of the hollow connecting rod is detachably connected with a sample injection needle through a needle clamp locking member. The cell sampling device has two general positions. The first position is that the slider with the needle tip is moved to the bottom of the slide rail, which is used for the sampling process of single cells. The second position is to move the slider with the needle tip to the top of the slide rail. At this time, this position is used to remove the ionization needle and replace the sampling needle to realize the next single-cell sampling. Therefore, the two ends of the Z-axis moving slide rail are provided with limit blocks for restricting the displacement of the moving sample feeding mechanism. The limit blocks located at the bottom are the sampling state positioning blocks, and the limit blocks located above are the sampling state positioning blocks.

The support structure is a three-dimensional adjustment frame, and the bottom of the three-dimensional adjustment frame is connected to the bottom support connecting plate 1 through the bottom support connecting rod 2 . Specifically, in this embodiment, the X-axis displacement fixed rail 11 is connected to the upper surface of the bottom support connecting plate through the X-axis displacement platform connector 3, the X-axis moving slide rail 17 can slide on the X-axis displacement fixed rail 11, and the Y The axis displacement rail 13 is connected to the upper surface of the X-axis moving rail 17, the Y-axis moving rail 14 can slide on the Y-axis displacement rail 13, and the Y-axis moving rail 14 and the Z-axis displacement rail 16 pass 90 The Y-axis is connected with the Z-axis connecting support plate 15, and the Z-axis moving slide rail can slide on the Z-axis displacement fixed rail 16. In this embodiment, the three-dimensional adjustment frame is electronically controlled, which is convenient for accurate displacement, so it is also equipped with a The Y-axis transmission motor 12, the Z-axis transmission motor 18, and the like.

The hollow connecting rod is fixed on the bottom end fixing piece of the hollow connecting rod through the front end locking piece of the hollow connecting rod and the rear end locking piece of the hollow connecting rod, and the bottom fixing piece of the hollow connecting rod is arranged on the moving slide. on the block.

One of the side walls of the moving slider is provided with a hole, and the slider stop locking member is screwed on the hole, which is used to fix the moving slider at the preset position of the slide rail. During the moving process of the moving slide rail with the slider, when the needle moves to a specific position, if you want to keep the needle tip position indefinitely, you can connect the slider with the slider to stop the locking piece and rotate clockwise. At this time, the slider connected with the spray needle will be fixed at a specific position, and the sampling needle can be replaced at this time, which is more convenient to operate.

The operating instrument of the present invention is divided into two operating processes. One is single-cell sampling. In this mode, the moving slider with the sampling needle tip is placed at the bottom of the slide rail, and the slider is prevented from falling down by the slider block. Manual or electronic control enables the three-dimensional adjustment frame to drive the sampling needle tip. Displacement, realize the micro-manipulation sampling of single cells under microscope observation, and can sample specific parts of single cells. When placed in the sampling position, the micro-nano manipulation needle tip is used to realize the micro-manipulation of single-cell samples, and the cells are extracted by negative pressure. internal samples. The second is the quick needle replacement mode. First, adjust the three-dimensional micro-manipulation slide rail. At this time, let the slide rail move to the reference position. The sampling needle tip has been slightly lifted from the cell culture solution. By moving the slider, the position of the sampling needle tip is placed. on the top of the slide rail, and then fix the slide block somewhere on the slide rail with the top wire. The slider with the needle tip will not slide down to the bottom due to the effect of gravity, which facilitates the removal of the sampling needle, and the replacement of a new needle to continue the next round of operation. The sampling needle tip of the present invention is connected by a device with rapid extraction and self-locking locking, so that the single-cell sampling needle tip can be quickly replaced and the single-cell sample can be effectively ionized.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments can still be modified, or some or all of the technical features thereof can be equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the embodiments of the present invention. scope.

Claims (4)

1. The utility model provides an operation appearance for unicellular sample and spray needle quick replacement, a serial communication port, include workstation, bearing structure and fix the unicellular sampling device on bearing structure, unicellular sampling device has the angle of downward sloping with bearing structure, unicellular sampling device includes that moving slide rail and sliding connection move on moving slide rail and advance a kind mechanism, it includes the hollow connecting rod of removal slider and fixed connection in its top to move a kind mechanism, the front end of hollow connecting rod is connected with a kind spray needle through double-layered needle retaining member detachably, the both ends of moving slide rail are equipped with the stopper that is used for the restriction to remove a kind mechanism displacement, and the stopper that is located the below is the sampling state locating piece, and the stopper that is located the top is the sampling state locating piece.

2. The manipulator of claim 1, wherein the support structure is a three-dimensional adjusting frame, and the bottom of the three-dimensional adjusting frame is connected to the bottom support connection plate through a bottom support connection rod.

3. The manipulator according to claim 1 or 2, wherein said hollow connecting rod is fixed to a hollow connecting rod bottom end fixture by a hollow connecting rod front end fixture and a hollow connecting rod rear end fixture, said hollow connecting rod bottom end fixture being disposed on the movable slide.

4. The manipulator for single cell sampling and rapid needle change according to claim 1 or 2, wherein one of the side walls of the movable slide is provided with a hole, and a slide stop lock is screwed at the hole and used for fixing the movable slide at a preset position of the slide rail.

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