CN114518462A - Sample analyzer and sample analyzing method - Google Patents

Abstract

A sample analyzer and sample analysis method, the sample analyzer includes the substrate and dispenses the structure, measuring mechanism, ultrasonic apparatus and controller, the substrate dispenses the structure and is used for pouring into the substrate to hold in the reaction liquid in the cup, the measuring mechanism is used for determining the reaction liquid, the ultrasonic apparatus is used for producing the ultrasonic vibration and forming the supersonic wave; the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the reaction liquid filled with the substrate in the containing cup. Because the sample analyzer is equipped with the ultrasonic device, the ultrasonic device is arranged in launching the ultrasonic wave to the reaction liquid of the injection substrate that holds the cup, and the ultrasonic wave can be with the homogeneous dispersion of the agglomeration material in the reaction liquid for the substrate can fully mark the reaction complex in the reaction liquid, avoids leaking the mark, and then can improve the accuracy of detecting.

Description

Sample analyzer and sample analysis method

technical field

The invention relates to in vitro detection equipment, in particular to a sample analyzer and a sample analysis method.

Background technique

Immunoanalyzer is a kind of high-sensitivity and high-specificity analytical instrument, which is often used to detect various analytical indicators of blood, urine or other body fluids in clinical laboratories. Traditional immunoassay analyzers have a variety of working principles, including chemiluminescence, electrochemiluminescence, and so on. Taking the heterogeneous chemiluminescence immunoassay analyzer as an example, its main working principle is: when a certain component in the sample needs to be measured, the corresponding antibody/antigen can be coated on the magnetic beads to form a magnetic bead reagent, and the specific marker can be coated. The label forms the labeling reagent on the antibody. During the test, the sample to be tested is mixed with magnetic bead reagents, labeling reagents and other reagents to form a reaction solution, and incubated under certain conditions to form a reaction complex, and then the labels bound to the magnetic bead reagent are extracted by magnetic separation technology. Finally, the substrate is added, and the substrate reacts with the label on the reaction complex to emit light, and then the detection result is obtained by photometry. However, in the process of testing, agglomerates are sometimes generated, which affect the labeling luminescence results of the substrates, resulting in inaccurate final detection results.

SUMMARY OF THE INVENTION

In one embodiment, a sample analyzer is provided, comprising:

Substrate dispensing structure for injecting the substrate into the reaction solution in the holding cup;

The measuring mechanism is used to measure the reaction solution;

an ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and

A controller is connected to the ultrasonic device, and the controller is used to control the ultrasonic device to emit ultrasonic waves into the reaction solution injected into the substrate in the holding cup.

In one embodiment, the controller is configured to acquire test item parameters, and to match an ultrasonic mode from a plurality of preset ultrasonic modes according to the test item parameters to perform an ultrasonic mixing operation on the reaction solution injected into the substrate.

In one embodiment, the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively.

In an embodiment, the ultrasonic device includes an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, the transmission member The first end is connected with the ultrasonic transducer, the outer diameter of the second end of the transmission member is smaller than the inner diameter of the accommodating cup; the moving device is connected with the ultrasonic transducer, and the moving device is used to drive the The ultrasonic transducer and the transmission member move relative to the accommodating cup, and the second end of the transmission member can be inserted into the reaction liquid injected into the substrate in the accommodating cup to vibrate the ultrasonic vibration generated by the ultrasonic transducer. It is transferred to the reaction solution injected into the substrate in the holding cup.

In one embodiment, the ultrasonic device includes an ultrasonic transducer and a transmission member, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, and the first end of the transmission member is The end is connected with the ultrasonic transducer; the second end of the transmission member is used to abut on the outer wall of the holding cup, and the part where the outer wall of the holding cup contacts the transmission member is a part surrounding the reaction liquid injected into the substrate part, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the reaction solution injected into the substrate in the holding cup.

In one embodiment, the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or decreases in steps from the first end to the second end.

In an embodiment, the sample analyzer further includes a reaction mechanism, the reaction mechanism is used to provide an incubation place for the reaction solution injected with the substrate in the holding cup, and the controller is used to control the injection substrate to be incubated by the ultrasonic device. Perform ultrasonic mixing of the reaction solution and/or the reaction solution injected with the substrate after incubation.

In one embodiment, the controller is configured to control the ultrasonic device to perform an ultrasonic mixing operation in the reaction mechanism for the substrate-infused reaction solution to be incubated and/or the incubated substrate-infused reaction solution.

In one embodiment, the sample analyzer further includes a magnetic separation mechanism and a transfer mechanism, the magnetic separation mechanism is used to perform a magnetic separation operation on the reaction liquid, and the substrate dispensing structure is used to transfer the magnetic separation mechanism to the magnetic separation mechanism. A substrate is injected into the magnetically separated reaction solution, and the transfer mechanism is used to transfer the holding cup between the magnetic separation mechanism and the reaction mechanism;

The controller is used to control the ultrasonic device to perform ultrasonic mixing on the reaction solution of the substrate injected to be incubated on the magnetic separation mechanism, and/or to perform ultrasonic mixing on the reaction mechanism after the incubation. operate.

In one embodiment, the sample analyzer further includes a magnetic separation mechanism and a transfer mechanism, the magnetic separation mechanism is used to perform a magnetic separation operation on the reaction liquid, and the substrate dispensing structure is used to transfer the magnetic separation mechanism to the magnetic separation mechanism. Substrate is injected into the reaction solution after magnetic separation, the reaction mechanism and the magnetic separation mechanism are provided with a mixing station, and the transfer mechanism is used for the reaction mechanism, the magnetic separation mechanism and the mixing station. Transfer holding cups between positions;

The ultrasonic device is used for performing an ultrasonic mixing operation on the reaction solution injected with the substrate to be incubated and/or the reaction solution injected with the substrate after incubation at the mixing position.

In one embodiment, a sample analysis method is provided, comprising the following steps:

The sample dispensing mechanism and the reagent dispensing mechanism respectively inject samples and reagents into the holding cup to form a reaction solution;

The reaction solution is incubated in the reaction mechanism;

The magnetic separation mechanism performs a magnetic separation operation on the incubated reaction solution;

The substrate dispensing structure injects the substrate into the magnetically separated reaction solution in the holding cup;

The reaction solution injected with the substrate is incubated in the reaction mechanism;

The ultrasonic device emits ultrasonic waves into the reaction solution injected into the substrate;

The measuring mechanism performs optical measurement on the reaction solution.

In one embodiment, the ultrasonic mixing operation of the ultrasonic device is controlled by the following steps:

Get test project parameters;

According to the test item, the parameters match one ultrasonic mode from a plurality of preset ultrasonic modes to perform ultrasonic mixing operation on the reaction solution injected into the substrate.

In one embodiment, the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively.

In one embodiment, the ultrasonic device performs an ultrasonic mixing operation on the substrate-infused reaction solution to be incubated and/or the incubated substrate-infused reaction solution.

In one embodiment, the ultrasonic device performs an ultrasonic mixing operation in the reaction mechanism for the substrate-infused reaction solution to be incubated and/or the incubated substrate-infused reaction solution.

In one embodiment, the ultrasonic device performs ultrasonic mixing on the magnetic separation mechanism for the substrate-injected reaction solution to be incubated, and/or on the reaction mechanism for the incubated substrate-injected reaction solution. operate.

In an embodiment, between the step of injecting the substrate into the magnetically separated reaction solution in the holding cup by the substrate dispensing structure and the incubation of the substrate-injected reaction solution in the reaction mechanism, the method further includes: Follow the steps below:

The transfer mechanism transfers the holding cup containing the reaction solution injected into the substrate from the magnetic separation mechanism to the mixing position;

The ultrasonic device performs an ultrasonic mixing operation on the reaction solution injected into the substrate on the mixing position;

The transfer mechanism transfers the accommodating cup containing the sonicated substrate-infused reaction solution from the mixing station to the reaction mechanism.

In an embodiment, the step between the incubation of the reaction solution injected with the substrate in the reaction mechanism and the optical measurement of the reaction solution by the assay mechanism in the step further includes the following steps:

The transfer mechanism transfers the accommodating cup containing the incubated reaction solution injected with the substrate from the reaction mechanism to the mixing position;

The ultrasonic device performs an ultrasonic mixing operation on the incubated reaction solution injected with the substrate on the mixing position;

The transfer mechanism transfers the accommodating cup containing the sonicated substrate-infused reaction solution from the mixing station to the reaction mechanism.

According to the sample analyzer and the sample analysis method of the above-mentioned embodiments, since the sample analyzer is provided with an ultrasonic device, the ultrasonic device is used to transmit ultrasonic waves into the reaction solution injected into the substrate in the holding cup, and the ultrasonic waves can agglomerate the aggregates in the reaction solution. The substances are uniformly dispersed, so that the substrate can fully label the reaction complex in the reaction solution, so as to avoid missing labels, thereby improving the detection accuracy.

Description of drawings

1 is a schematic structural diagram of an immunoluminescence analyzer in an embodiment;

Fig. 2 is a structural block diagram of the control part of the immunoluminescence analyzer in an embodiment;

3 is a schematic structural diagram of a vortex mixing device in an embodiment;

4 is a schematic structural diagram of a contact ultrasonic device in an embodiment;

FIG. 5 is a structural view of a transmission member in an embodiment;

FIG. 6 is a structural view of a transmission member in an embodiment;

7 is a schematic diagram of a mobile device in an embodiment;

8 is a schematic diagram of ultrasonic mixing in an embodiment;

9 is a schematic structural diagram of a non-contact ultrasonic device in an embodiment;

10 is a schematic structural diagram of a non-contact ultrasonic device in an embodiment;

Figure 11 is a side view of a gripping device in one embodiment;

FIG. 12 is a top view of a gripping device in one embodiment;

13 is a sequence diagram of a sample analysis method in an embodiment;

14 is a flowchart of a sample analysis method in one embodiment;

Figure 15 is a comparison diagram of the test results of ultrasonic mixing and non-ultrasonic mixing in the sample analysis process in one embodiment;

16 is a flowchart of a sample analysis method in one embodiment;

Figure 17 is a flow diagram of a sample analysis method in one embodiment.

Detailed ways

The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments have been associated with similar element numbers. In the following embodiments, many details are described so that the present application can be better understood. However, those skilled in the art will readily recognize that some of the features may be omitted under different circumstances, or may be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application from being overwhelmed by excessive description, and for those skilled in the art, these are described in detail. Correlation operations are not necessary, and they can be completely de-correlated operations based on descriptions in the specification and general technical knowledge in the field.

Additionally, the features, acts, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can also be exchanged or adjusted in order in a manner obvious to those skilled in the art. Accordingly, the various sequences in the specification and drawings are merely for the purpose of clearly describing a certain embodiment and are not meant to be a required order unless otherwise stated where a certain order must be followed.

The serial numbers themselves, such as "first", "second", etc., for the components herein are only used to distinguish the described objects, and do not have any order or technical meaning. The "connection" and "connection" mentioned in this application, unless otherwise specified, include both direct and indirect connections (connections).

For the immunoluminescence analyzer, the one-step test item in the present invention means that one test item only needs to be incubated in one step; correspondingly, the multi-step test item means that one test item needs to be incubated with multiple steps For example, a two-step test item means that the test item requires two-step incubation. First, add the reagents required for the first-step incubation to the sample, and then perform the first-step incubation. After the first-step incubation time is reached, then Add the reagents required for the second step of incubation, and then perform the second step of incubation. After the second step of incubation time, perform another magnetic separation, and then perform the measurement. Generally speaking, in a multi-step test project, magnetic separation needs to be performed after the last step of incubation before the measurement can be performed; while in a multi-step test project, in addition to the last step of incubation, whether magnetic separation is required after other steps of incubation The separation depends on factors such as the type of test items. For example, in a two-step test item, if magnetic separation is required after incubation in the first step of the test, the two-step test item can be called a two-step, two-separation test item. Magnetic separation is not required after incubation, so the two-step test item can be called a two-step-one-separation test item.

In a one-step test item or a multi-step test item, for each step of incubation or each incubation, the types of reagents that need to be added can be one or more, which are determined according to factors such as the type of test items When there are one-step or multi-step tests in a one-step test item or a multi-step test item, and there are multiple types of reagents to be added for incubation, this test item can be called a multi-component test project.

In one embodiment, a sample analyzer is provided. The sample analyzer is provided with an ultrasonic device, and the reaction solution is ultrasonically mixed by the ultrasonic device, so as to improve the accuracy of item detection. The sample analyzer may be a biochemical analyzer or an immunological analyzer, and this embodiment takes an immunoluminescence analyzer as an example for description.

1 and 2, the immunoluminescence analyzer mainly includes an ultrasonic device 10, a sample carrying mechanism 21, a sample dispensing mechanism 22, a reagent carrying mechanism 31, a reagent dispensing mechanism 32, a reaction mechanism 40, a magnetic separation mechanism 50 and a control device 60. The sample carrying mechanism 21 , the sample dispensing mechanism 22 , the reagent carrying mechanism 31 , the reagent dispensing mechanism 32 , the reaction mechanism 40 , the magnetic separation mechanism 50 and the ultrasonic device 10 are all installed on the base 100 , and the controller 60 is installed on the base 100 The controller 60 can also be installed on the base 100. The above-mentioned magnetic separation mechanism is not included in the biochemical analyzer.

The immunoluminescence analyzer also includes a cup-upper mechanism 71 , a cup-throwing position 72 , a first transfer mechanism 81 , a second transfer mechanism 82 and a measurement mechanism 90 installed on the base 100 .

The reaction mechanism 40 is arranged in the middle, and the reagent carrying mechanism 31 , the magnetic separation mechanism 50 , the ultrasonic device 10 , the cup upper mechanism 71 , the cup throwing position 72 and the measuring mechanism 90 are respectively arranged around the reaction mechanism 40 .

The cup-upper mechanism 71 is used to store a new accommodating cup 103 that has not been used, and the accommodating cup 103 is also called a reaction cup. The cup-upper mechanism 71 itself also has a cup-shifting function, which can transfer the accommodating cup 103 from the storage position to the position to be grasped.

The first transfer mechanism 81 is arranged between the cup-upper mechanism 71 and the reaction mechanism 40 . The first transfer mechanism 81 is a cup grabbing mechanism, and the first transfer mechanism 81 is used to transfer the new accommodating cup 103 on the cup-upper mechanism 71 to a position close to the reaction mechanism. on the sample adding position 101 of the mechanism 40 , and transfer the accommodating cup 103 on the sample adding position 101 into the reaction mechanism 40 .

The cup throwing position 72 is located within the moving range of the first transfer mechanism 81 , the cup throwing position 72 is connected to the recovery box, and the cup throwing position 72 is used to recover the used accommodating cup 103 . The first transfer mechanism 81 is also used for transferring the accommodating cup 103 detected on the reaction mechanism 40 to the cup throwing position 72 .

The sample carrying mechanism 21 is used to carry the sample. In some examples, the sample carrying mechanism 21 may include a sample distribution module (SDM, Sample Delivery Module); in other examples, the sample carrying mechanism 21 may also be a sample tray, and the sample tray includes a plurality of sample positions such as sample tubes. By rotating the disk-like structure of the disk, the sample can be dispatched to a corresponding position, such as a position for the sample dispensing mechanism 22 to draw the sample.

The sample dispensing mechanism 22 includes a sampling needle, a moving mechanism and a driving pump. The moving mechanism is used to drive the sampling needle to move two-dimensionally or three-dimensionally between the sample carrying mechanism 21 and the sample adding position 101, and the driving pump is used to provide suction for the sampling needle. Kind and spit motivation. The sample dispensing mechanism 22 is used for sucking the sample in the sample tube on the sample carrying mechanism 21 , and for filling the sucked sample into the accommodating cup 103 on the sample filling position 101 .

The reagent carrying mechanism 31 is used for carrying reagents. In one embodiment, the reagent carrying mechanism 31 may be a reagent disk, the reagent disk is arranged in a disc-like structure and has multiple positions for carrying the reagent containers, and the reagent carrying mechanism 31 can rotate and drive the reagent containers it carries to rotate, It is used to rotate the reagent container to a specific position, for example, the position where the reagent is sucked by the reagent dispensing mechanism 32 . The number of reagent parts 13 may be one or more.

The reagent dispensing mechanism 32 includes a reagent needle, a moving mechanism and a driving pump. The moving mechanism is used to drive the reagent needle to move two-dimensionally or three-dimensionally between the reagent carrying mechanism 31 and the reaction mechanism 40, and the driving pump is used to provide the reagent needle with suction reagents And the power to spit reagents. The reagent dispensing mechanism 32 is used for absorbing the reagent in the reagent tube on the reagent carrying mechanism 31, and for filling the sucked reagent into the accommodating cup 103 containing the sample on the reaction mechanism 40, and the sample in the accommodating cup 103 and the reagent are mixed and reacted A reaction solution is formed.

The reaction mechanism 40 is used to provide an incubation place for the reaction liquid. The reaction mechanism 40 can be a reaction disk, which is arranged in a disc-shaped structure and has one or more placement positions for placing the reaction cup. The reaction disk can rotate and drive the reaction cup. The cuvette in the placement position is rotated for scheduling the cuvette in the reaction tray and incubating the reaction solution in the cuvette.

The magnetic separation mechanism 50 includes a cleaning liquid dispensing structure, a magnetic suction structure, a liquid suction structure and a substrate dispensing mechanism. The cleaning liquid dispensing structure is used to inject the cleaning liquid into the reaction solution after incubation, and the cleaning liquid is used to inject the cleaning liquid into the incubated reaction liquid. The free substances in the reaction solution after incubation are separated; the magnetic suction structure is used to perform the magnetic suction operation on the reaction solution filled with the cleaning solution, and the magnetic suction structure is used to adsorb the reaction complexes bound to the magnetic beads; the suction structure is used for The other components except the reaction complex bound to the magnetic beads are discharged from the holding cup 103; the substrate dispensing mechanism is used to add the substrate into the reaction liquid in the holding cup 103, and the reaction between the substrate and the reaction liquid The complex reacts, and the substrate labels the reaction complex luminescently.

Two magnetic separation mechanisms 50 are provided, and the two magnetic separation mechanisms 50 can work independently of each other, so as to improve the efficiency of the whole machine test.

The second transfer mechanism 82 is installed between the reaction mechanism 40 and the magnetic separation mechanism 50, and a mixing position 102 is provided at a position close to the reaction mechanism 40 and the magnetic separation mechanism 50. Both the mixing position 102 and the sample adding position 101 are provided with For the cup holder on which the accommodating cup 103 is placed, the second transfer mechanism 82 is used to transfer the accommodating cup 103 among the reaction mechanism 40 , the magnetic separation mechanism 50 and the mixing station 102 .

The measurement mechanism 90 is used to perform optical measurement on the reaction solution after incubation to obtain reaction data of the sample. For example, the measuring mechanism 90 detects the luminescence intensity of the reaction solution to be measured, and calculates the concentration of the component to be measured in the sample based on the calibration curve.

The machine base 100 is further provided with a cleaning mechanism and a waste liquid suction mechanism, the cleaning mechanism is used for cleaning the sampling needle and the reagent needle, and the waste liquid suction mechanism is used for suctioning the detected reaction liquid.

The ultrasonic device 10 is installed near the mixing position 102 , and the ultrasonic device 10 is used to perform ultrasonic mixing operation on the reaction solution located in the holding cup 103 on the mixing position 102 .

In this embodiment, the second transfer mechanism 82 is used to transfer the holding cup 103 containing the reaction solution injected into the substrate from the magnetic separation mechanism 50 to the mixing position 102 , and the ultrasonic device 10 is used to transfer the bottom of the mixing position 102 to the mixing position 102 . The reaction solution of the substrate is subjected to ultrasonic mixing operation, and the second transfer mechanism 82 is also used to transfer the holding cup 103 containing the reaction solution injected into the substrate after ultrasonic mixing from the mixing position 102 to the reaction mechanism 40 .

In one embodiment, the second transfer mechanism 82 is used to transfer the holding cup 103 containing the reaction solution injected with the substrate from the magnetic separation mechanism 50 to the reaction mechanism 40, and the second transfer mechanism 82 is also used to transfer the container containing the incubation The accommodating cup 103 of the substrate-injected reaction solution is transferred from the reaction mechanism 40 to the mixing station 102 , and the ultrasonic device 10 is used to perform ultrasonic mixing on the substrate-injected reaction solution incubated on the mixing station 102 .

In one embodiment, the second transfer mechanism 82 is used to transfer the holding cup 103 containing the reaction solution injected into the substrate from the magnetic separation mechanism 50 to the mixing position 102 , and the ultrasonic device 10 is used to inject the reaction liquid on the mixing position 102 . The reaction solution of the substrate is subjected to ultrasonic mixing operation, and the second transfer mechanism 82 is also used to transfer the holding cup 103 containing the reaction solution injected into the substrate after ultrasonic mixing from the mixing position 102 to the reaction mechanism 40; The second transfer mechanism 82 is also used to transfer the accommodating cup 103 containing the reaction solution injected into the substrate after incubation from the reaction mechanism 40 to the mixing station 102 , and the ultrasonic device 10 is used to re-incubate the injection on the mixing station 102 after incubation. The reaction solution of the substrate is subjected to ultrasonic mixing operation, and the second transfer mechanism 82 is also used to transfer the holding cup 103 containing the reaction solution injected into the substrate after ultrasonic mixing again from the mixing position 102 to the reaction mechanism 40 .

In an embodiment, the ultrasonic device 10 is arranged close to the magnetic separation mechanism 50, or the ultrasonic device 10 is arranged in the magnetic separation mechanism 50, and the ultrasonic device 10 is used to perform an ultrasonic mixing operation on the reaction solution injected into the substrate in the magnetic separation mechanism 50. .

In one embodiment, the ultrasonic device 10 is disposed close to the reaction mechanism 40, or the ultrasonic device 10 is disposed in the reaction mechanism 40, and the ultrasonic device 10 is used to perform an ultrasonic mixing operation on the reaction solution injected into the substrate to be incubated in the reaction mechanism 40. .

In one embodiment, the ultrasonic device 10 is disposed close to the reaction mechanism 40, or the ultrasonic device 10 is disposed in the reaction mechanism 40, and the ultrasonic device 10 is used to perform an ultrasonic mixing operation on the reaction solution injected into the substrate after incubation in the reaction mechanism 40. .

In one embodiment, the ultrasonic device 10 is disposed close to the reaction mechanism 40, or the ultrasonic device 10 is disposed in the reaction mechanism 40, and the ultrasonic device 10 is used to perform an ultrasonic mixing operation on the reaction solution injected into the substrate to be incubated in the reaction mechanism 40. , the ultrasonic device 10 is also used for performing ultrasonic mixing operation on the reaction solution injected into the substrate after incubation in the reaction mechanism 40 .

In one embodiment, the ultrasonic device 10 is installed between the reaction mechanism 40 and the magnetic separation mechanism 50, and the ultrasonic device 10 can be moved into the magnetic separation mechanism 50 to perform ultrasonic mixing on the reaction solution injected into the substrate; and the ultrasonic device 10 It can be moved into the reaction mechanism 40 to perform ultrasonic mixing operation on the reaction solution injected with the substrate after incubation.

In the above embodiment, the ultrasonic device 10 is mainly used to perform ultrasonic mixing operation on the reaction solution injected into the substrate before and/or after incubation. Wherein, the ultrasonic device 10 performs an ultrasonic mixing operation on the reaction solution injected into the substrate before incubation, and the ultrasonic device 10 can evenly separate the components in the reaction solution, so that the substrate can fully label the reaction complex in the reaction solution, Thus, the detection accuracy is improved. The ultrasonic device 10 performs an ultrasonic mixing operation on the reaction solution injected into the substrate after incubation. The ultrasonic device 10 can uniformly disperse the aggregated substances produced in the reaction solution during the incubation process, thereby improving the accuracy of subsequent optical measurements.

In one embodiment, there are at least two mixing positions 102, and the placement positions of the accommodating cups 103 are increased, and one ultrasonic device 10 corresponds to at least two mixing positions 102, that is, one ultrasonic device 10 can respectively The reaction solution 104 in the holding cup 103 is subjected to ultrasonic mixing. During the ultrasonic mixing of one holding cup 103 , the holding cups 103 on the other mixing positions 102 can be transferred or other operations. The accommodating cups 103 realize alternate mixing to improve detection efficiency.

In one embodiment, the ultrasonic device 10 may also be set in a one-to-one correspondence with the mixing positions 102 .

Referring to FIG. 3, in an embodiment, a vortex mixing device 200 is also installed at the mixing position 102. The vortex mixing device 200 includes a driving motor 201, a transmission belt 202, an eccentric rotating shaft 203 and a mounting seat 204, etc. The driving motor 201 is fixedly installed on the mounting seat 204, the output shaft of the driving motor 201 is arranged downward, the eccentric rotating shaft 203 is rotatably installed on the mounting seat 204 through a bearing, the eccentric rotating shaft 203 is vertically arranged, and the eccentric rotating shaft 203 has a non-collinear first. One section and the second section, the first section is located at the lower position, the second section is located at the upper position, and the first and second sections of the eccentric shaft 203 are both parallel to the output shaft of the drive motor 201 . Pulleys are installed on the output shaft of the drive motor 201 and on the first section of the eccentric shaft 203 respectively. The drive belt 202 is connected to the pulley of the drive motor 201 and the eccentric shaft 203. The drive motor 201 drives the eccentric shaft 203 to rotate through the drive belt 202. The eccentric rotating shaft 203 is installed with a cup holder 205 for placing the holding cup 103 , and the eccentric rotating shaft 203 can drive the holding cup 103 located on the cup holder 205 to rotate eccentrically, and perform a vortex mixing operation on the reaction liquid in the holding cup 103 . The drive motor 201 is connected to the controller 60, and the controller 60 controls the output power and output duration of the drive motor 201 to realize various vortex mixing modes with different intensities and times.

In this embodiment, the controller 60 is respectively connected with the ultrasonic device 10 , the sample dispensing mechanism 22 , the reagent dispensing mechanism 32 , the reaction mechanism 40 , the magnetic separation mechanism 50 , the first transfer mechanism 81 and the second transfer mechanism 82 . 60 is used to control the test sequence of the entire sample analyzer.

When the controller 60 controls the ultrasonic device 10, the controller 60 obtains the test items input or selected by the doctor, and obtains the test item parameters corresponding to the test items, and matches an ultrasonic mode response from a plurality of ultrasonic modes according to the test item parameters. The liquid was mixed by ultrasonic.

Different ultrasonic modes have different ultrasonic mixing intensities or different ultrasonic mixing times. The mixing intensity is controlled by the input power. It can be set to include at least three ultrasonic mixing intensities: strong, medium and weak, and can be set to include at least 1s. and 2s two ultrasonic mixing times.

Ultrasonic mixing modes include at least the following:

In the first mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is medium, and the ultrasonic mixing time is 1 s;

In the second mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is weak, and the ultrasonic mixing time is 2s;

In the third mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is strong, and the ultrasonic mixing time is 1 s.

The test item parameters include numbers, letters, or a combination of the two. For example, the test item parameter of the TNI (troponin) item is 2, and the test item parameter of the E2 (estradiol) item includes 0 and 1. The controller 60 pre-stores item test parameters corresponding to different test items, and each item test parameter corresponds to a mixing mode. If the test item parameter 0 corresponds to the first mixing mode, when the test item parameter obtained by the controller 60 is 0, the ultrasonic device 10 is driven to perform ultrasonic mixing with a medium intensity and a time of 1 s on the reaction solution; test Item parameter 1 corresponds to the second mixing mode. When the test item parameter obtained by the controller 60 is 1, the ultrasonic device 10 is driven to perform ultrasonic mixing with weak intensity and 2s time on the reaction solution; the test item parameter 2 corresponds to the third mixing mode. When the parameter of the test item obtained by the controller 60 is operation 2, the ultrasonic device 10 is driven to perform ultrasonic mixing with a strong intensity and a time of 1 s on the reaction solution.

Different ultrasonic mixing modes can be set according to specific test items, so that the ultrasonic device 10 can effectively ultrasonically mix the reaction solution in different test items.

In one embodiment, there are multiple ultrasonic devices 10 , and one ultrasonic device 10 is disposed close to the sample adding position 101 , so that the ultrasonic device 10 can perform ultrasonic mixing on the sample, reagent or reaction solution in the holding cup on the sample adding position 101 . uniform.

In one embodiment, there are multiple ultrasonic devices 10 , and one ultrasonic device 10 is disposed close to the reagent carrying mechanism 31 , so that the ultrasonic device 10 can ultrasonically mix the reagents in the reagent carrying mechanism 31 .

Referring to FIG. 4 , in this embodiment, the ultrasonic device 10 is a device independent of other mechanisms, that is, the ultrasonic device 10 can operate independently. For example, the ultrasonic device 10 can operate independently of the sample dispensing mechanism 22 . Run synchronously or asynchronously to improve the efficiency of item detection.

The ultrasonic device 10 is a contact ultrasonic device. The ultrasonic device 10 includes an ultrasonic transducer 11, a transmission member 12 and a moving device 13. The ultrasonic transducer 11 includes a backing layer, a piezoelectric layer and a matching layer that are connected in sequence. The layer is a piezoelectric crystal, and the piezoelectric crystal produces compression and expansion in the thickness direction through the inverse piezoelectric effect under the action of the driving electrical signal. The frequency of this deformation reaches the ultrasonic frequency, forming ultrasonic vibration.

Please refer to FIG. 4 and FIG. 5 , the transmission member 12 is a solid rod-shaped structure, and the transmission member 12 has a first end and a second end, wherein the first end is the upper end, the second end is the lower end, and the first end of the transmission member 12 There is an external thread, the lower end of the ultrasonic transducer 11 is provided with an internal thread, and the transmission member 12 is installed on the lower end of the ultrasonic transducer 11 by means of screw connection. device 11 is connected. The transmission member 12 is a resonant rod, the transmission member 12 is connected with the matching layer of the ultrasonic transducer 11 , and the transmission member 12 is used for transmitting ultrasonic vibration. Compared with the transmission member 12 with a hollow structure, the solid transmission member 12 is conducive to the transmission of axial vibration, and when the outer diameter of the transmission member 12 decreases along the direction of ultrasonic vibration transmission, the solid transmission member 12 is conducive to the convergence of energy , in order to achieve better ultrasonic mixing effect.

The outer diameter of the transmission member 12 gradually decreases or decreases in steps from the first end to the second end, and the transmission member 12 has the function of concentrating energy. When the ultrasonic vibration is transmitted from the first end to the second end, the second end is opposite to the first end. The axial cross-sectional area of one end is reduced, and the ultrasonic vibration is more concentrated at the second end relative to the first end, so that the second end of the transmission member 12 amplifies the amplitude of the outgoing ultrasonic vibration relative to the first end, thereby increasing the outgoing ultrasonic energy. .

Specifically, the transmission member 12 includes a first end 121 , a middle section 122 and a second end 123 , wherein the first end 121 is a connection end with an external thread, the second end 123 is a needle bar structure, and the outer end of the second end 123 The diameter is smaller than the inner diameter of the receiving cup 103 so that the second end 123 of the transmission member 12 can be inserted into the receiving cup 103 . The middle section 122 is a horn-shaped structure, the end of the middle section 122 connected with the first end 121 is the big end of the horn, the end of the middle section 122 connected with the second end 123 is the small end of the horn, and the middle section 122 is from the big end of the horn to the small end of the horn. The shaft diameter of the end gradually decreases.

The middle section 122 can also be composed of one or any combination of cylindrical rods and conical rods. Please refer to FIG. 6 , the middle section 122 of structure a includes two sections of cylindrical rods with different diameters; the middle section 122 of structure b includes four sections of cylindrical rods with different diameters; the middle section 122 of structure c includes a section of conical rods; the middle section 122 of structure c includes a section of conical rods; The middle section 122 includes two sections of cylindrical rods of different diameters and a section of conical rods. The above five structures of the transmission member 12 are all structures that gradually decrease or stepwise decrease from the first end to the second end, and can play a role of amplifying the amplitude.

Referring to FIG. 7 , the mobile device 13 includes a mounting base 131 , a swing arm assembly 132 , a first moving assembly 133 and a second moving assembly 134 .

The swing arm assembly 132 includes a swing arm 1321 and a lift rod 1322, the lift rod 1322 is vertically movably liftable and rotatable mounted on the mounting seat 131, the swing arm 1321 is arranged horizontally, one end of the swing arm 1321 is connected to the lift rod 1322, and the ultrasonic The transducer 11 is mounted on one end of the swing arm 1321 away from the lift rod 1322 . The swing arm assembly 132 is used to drive the vertical lift and horizontal rotation of the ultrasonic transducer 11 and the transmission member 12 . In one embodiment, the swing arm 1321 and the lift rod 1322 can also be an integrated structure.

The first moving assembly 133 is a lifting assembly. The first moving assembly 133 includes a lifting motor 1331 and a lifting transmission assembly 1332. The lifting motor 1331 is installed on the mounting seat 131. The lifting transmission assembly 1332 includes a transmission wheel, a transmission belt, a gear and a rack, and the toothed The bar is vertically installed on the lifting rod 1322, the gear is rotatably mounted on the mounting seat 131, the gear is meshed with the rack, the lifting motor 1331 is connected with the gear through a transmission wheel and a transmission belt, and the lifting motor 1331 drives the lifting rod through the rack and pinion 1322 Lift and move. In one embodiment, the first moving component 133 is a linear motor, and the output shaft of the linear motor is directly connected with the lift rod 1322 , and can also drive the lift rod 1322 to move up and down.

The second moving assembly 134 is a rotating assembly. The second moving assembly 134 includes a rotating motor 1341 and a rotating transmission assembly 1342. The rotating motor 1341 is mounted on the mounting seat 131. The rotating transmission assembly 1342 includes a transmission belt and a rotating gear. The gear is sleeved on the lifting rod 1322, the rotating gear is connected with the lifting rod 1322 by a key, the lifting rod 1322 can move up and down relative to the rotating gear, the rotating gear is used to drive the lifting rod 1322 to rotate, the rotating motor 1341 is connected with the rotating gear through a transmission belt, and the rotating motor 1341 is used to drive the lift rod 1322 to rotate. In one embodiment, the rotating motor 1341 is connected to the lifting rod 1322 through a gear set, and can also drive the lifting rod 1322 to rotate.

In one embodiment, the moving device 13 only includes the mounting seat 131 , the swing arm assembly 132 and the first moving assembly 133 , the ultrasonic device 10 has a lifting function, and the ultrasonic device 10 is used to adjust the mixing position 102 on the accommodating cup 103 . The reaction solution 104 performs a mixing operation.

In one embodiment, the second moving component 134 can also be a plane moving component composed of X-axis movement and Y-axis movement. The member 12 moves alternately among the plurality of mixing positions 102 .

Referring to FIG. 8 , in this embodiment, the transmitting member 12 of the ultrasonic device 10 is directly inserted into the reaction solution 104 in the accommodating cup 103 . The ultrasonic device 10 has a preset frequency and voltage, so that the ultrasonic vibration mainly propagates along the axial direction, and the second end face of the transmitting member 12 is the emitting surface of the ultrasonic wave. During ultrasonic mixing, the second end face of the transmission member 12 emits ultrasonic waves into the reaction solution 104, an ultrasonic sound field is formed in the reaction solution 104, and the reaction solution 104 will form a violent liquid flow under the action of the ultrasonic sound field to realize the reaction solution. Mixing of the ingredients in 104.

In addition to the vibration effect of the ultrasonic waves, which can realize the mixing of the reaction liquid 104 , the cavitation effect generated by the ultrasonic waves in the liquid can also uniformly disperse some agglomerated and adhered substances in the reaction liquid 104 . When the frequency and sound pressure of the ultrasonic waves are controlled, combined with the amplification effect of the transmission member 12, the ultrasonic energy entering the reaction liquid 104 in the holding cup 103 is greater than the threshold of ultrasonic cavitation, then during the ultrasonic mixing process, the reaction liquid can be mixed in the reaction liquid. Ultrasonic cavitation occurs in 104. When ultrasonic cavitation occurs, a large amount of energy will be released to generate a certain force on some agglomerated and adhered substances in the reaction liquid 104 to disperse them. At the same time, under the action of ultrasonic vibration and mixing, these substances can be uniformly dispersed in the in the reaction cup.

In one embodiment, two or more mixing positions 102 are provided to increase the placement positions of the holding cups 103, so that during the ultrasonic mixing process of one holding cup 103, the other holding cups 103 can be transferred or other operations. The accommodating cups 103 on the multiple mixing positions 102 are alternately mixed in solid form, so as to improve the detection efficiency.

A plurality of mixing positions 102 are arranged on a circumference centered on the rotation center of the second moving assembly 133, so that the second moving assembly 133 can drive the transmission member 12 to move alternately among the plurality of mixing positions 102, and then an ultrasonic device 10. Ultrasonic mixing can be performed on the reaction solutions 104 in the plurality of holding cups 103 respectively.

Please refer to FIG. 9 and FIG. 10 , the ultrasonic device 10 includes an ultrasonic transducer 11 and a transmission member 12 . During ultrasonic mixing, the second end of the transmitting member 12 abuts the outer wall of the holding cup 103 , and the ultrasonic vibration is transmitted into the reaction liquid 104 through the holding cup 103 . Since the transmission member 12 does not need to be inserted into the receiving cup 103, the axial length of the transmission member 12 is shorter than that of the contact type transmission member, but it also has a gradual or stepped reduction from the first end to the second end. features to achieve amplified amplitude.

During ultrasonic mixing, the second end face of the transmission member 12 in this embodiment abuts on the outer wall of the accommodating cup 103, and the part of the outer wall of the accommodating cup 103 in contact with the transmission member 12 is the part surrounding the reaction liquid 104, so that the ultrasonic The ultrasonic vibration generated by the transducer 11 is transmitted to the liquid in the holding cup 103 . The part of the accommodating cup 103 surrounding the reaction liquid 104 is the bottom of the accommodating cup 103 and the lower end sidewall connected to the bottom, so the second end of the transmission member 12 abuts against the bottom of the accommodating cup 103 and any of the lower end sidewalls connected to the bottom. All positions are capable of transmitting ultrasonic vibrations into the liquid in the holding cup 103 .

In this embodiment, the ultrasonic device 10 is a movable structure. The ultrasonic device 10 further includes a moving device. The moving device includes a mounting seat and a horizontal moving component. The horizontal moving component is installed on the mounting seat, and the ultrasonic transducer is installed on the horizontal moving component. , the horizontal moving component is an air cylinder or a linear motor, and the horizontal moving component is used to drive the second end of the transmission member 12 to abut or leave the outer wall of the accommodating cup 103 on the mixing position 102 .

In one embodiment, the ultrasonic device 10 is set as a fixed structure, and the transmission member 12 is located at a preset position, so that after the accommodating cup 103 is placed on the mixing station 102 , the accommodating cup 103 will directly contact the second end of the transmission member 12 .

In this embodiment, the sample analyzer further includes a holding device 110 , and the holding device 110 is used to limit the radial degree of freedom of the accommodating cup 103 on the mixing position 102 .

Please refer to FIG. 11 and FIG. 12 , the gripping device 110 includes two gripping assemblies symmetrically arranged relative to the mixing position 102 , each gripping assembly includes a gripping motor 111 , a gripping cam 112 and a gripping clamping block 113 . The tightening motor 111 is installed on the machine base 100, the output shaft of the tightening motor 111 is vertically arranged upward, the output shaft of the tightening motor 111 is connected with the tightening cam 112 in a transmission connection, the tightening cam 112 is arranged horizontally, and the tightening cam 112 is connected with the tightening cam 112. The holding and clamping blocks 113 are in contact and connected, and the holding and clamping blocks 113 are installed on the machine base 100 movably horizontally. If the accommodating cup 103 is a circular tube, the surface of the clamping block 113 facing the accommodating cup 103 is a concave arc surface; if the accommodating cup 103 is a square tube, the surface of the clamping block 113 facing the accommodating cup 103 is a flat surface. The convex portion of the gripping cam 112 is a convex arc surface, so the surface of the gripping block 113 facing the gripping cam 112 is a concave arc surface with a larger curvature, so that the concave arc surface of the gripping block 113 can guide the gripping cam 112. The raised portion of the tightening cam 112 slides in and out. The gripping motor 111 is used to drive the gripping cam 112 to rotate, so that the gripping cam 112 drives the gripping clamping block 113 to approach or move away from the accommodating cup 103 . When they are on a line, the two holding clamp blocks 113 hold the holding cup 103 tightly, and the radial freedom of the holding cup 103 is limited, thereby avoiding the shaking of the holding cup 103 during the ultrasonic mixing process and ensuring the holding cup 103 Good contact with the transfer element 12 .

In this embodiment, since the ultrasonic device 10 is in contact with the lower end of the holding cup 103 to realize ultrasonic mixing, the holding clamping block 113 of the holding device 110 holds the side wall of the lower end of the holding cup 103 tightly to improve the holding stability . When the transmission member 12 of the ultrasonic device 10 abuts on the lower end sidewall of the accommodating cup 103 , the transmission member 12 and the clamping block 113 are arranged staggered from each other on the lower end sidewall of the accommodating cup 103 .

In one embodiment, the gripping device 110 may also include a linear driving member and a gripping clamping block. The linear driving member is an air cylinder or a linear motor. The limit of the accommodating cup 103 .

This embodiment adopts the non-contact ultrasonic device 10 , which can also transmit ultrasonic vibration to the reaction liquid 104 in the holding cup 103 to form an ultrasonic sound field and ultrasonic cavitation, so as to ultrasonically mix the reaction liquid 104 in the holding cup 103 .

In one embodiment, a sample analysis method is provided, and the sample analysis method is performed by the sample analyzer in the above embodiment.

Please refer to Figure 13. In the whole machine test, according to different reagent items, the sample analyzer mainly includes the following five different test procedures:

Test process 1. One-step separation: After adding sample S and reagent R respectively, carry out one incubation and one magnetic separation operation, and then carry out adding substrate A, incubation and photometry:

Test process 2. Two-step method for one-time separation: after adding sample S, add reagent R1 in the first step. Reagent R1 is one or more reagents, and the sample and reagent R1 are mixed to form a reaction solution for the first incubation; After the first incubation, the second step is to add reagent R2, which is one or more reagents, and the reagent R2 and the reaction solution after the first incubation form a new reaction solution for the second incubation; the second incubation The resulting reaction solution was subjected to magnetic separation, substrate A addition, incubation and photometry in sequence.

Test process 3. Two-step separation: after adding the sample, add reagent R1 in the first step, mix the sample and reagent R1 to form a reaction solution for the first incubation, and perform the first magnetic separation after the first incubation Operation; after the first magnetic separation operation, the second step is to add reagent R2, and the reagent R2 and the reaction solution after the first magnetic separation form a new reaction solution for the second incubation; the second incubation reaction solution is subjected to the first step. The second magnetic separation operation; the reaction solution after the second magnetic separation operation is sequentially added with substrate A, incubated and photometric.

Test process 4. Sample pretreatment: add sample S, then add pretreatment reagent, pretreatment reagent preprocesses the sample to form sample S'; add reagent R to the pretreated sample S', and then proceed in sequence Incubation, magnetic separation, addition of substrate A, incubation and photometry.

Test process 5. Sample pretreatment: add sample S, then add diluent, and the diluent will dilute the sample to obtain sample S' with lower concentration; add reagents to the diluted sample S', and then proceed in sequence Incubation, magnetic separation, addition of substrate A, incubation and photometry.

In this embodiment, the sample analysis method is controlled and executed by the controller 60, and the one-step magnetic separation is taken as an example for description. In the sample analysis method, the reaction solution injected into the substrate A to be incubated is mixed by ultrasonic.

Please refer to FIG. 14 , the sample analysis method of this embodiment includes the following steps:

S101: add sample;

The first transfer mechanism 81 transfers the new accommodating cup 103 on the cup-upper mechanism 71 to the sample adding position 101;

The sample dispensing mechanism 22 sucks the sample S from the sample carrying mechanism 21 , and fills the sucked sample S into the accommodating cup 103 on the sample adding position 101 .

S102: add reagent;

The first transfer mechanism 81 transfers the holding cup 103 containing the sample S from the sample adding position 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the holding cup 103 containing the reagent R to the reagent adding position;

The reagent dispensing mechanism 32 sucks the reagent R from the reagent carrying mechanism 31 , and fills the sucked reagent into the accommodating cup 103 at the reagent loading position in the reaction mechanism 40 , and the sample S and the reagent R in the accommodating cup 103 are mixed to form The reaction solution.

S103: vortex mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution to the mixing position 102;

A vortex mixing device is used to perform a vortex mixing operation on the reaction solution in the holding cup 103, so that the sample S and the reagent R can fully react.

S104: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the vortex-mixed reaction solution from the mixing position 102 back to the inner circle of the reaction mechanism 40 for incubation for a preset time.

S105: Magnetic separation;

The second transfer mechanism 82 transfers the holding cup 103 containing the incubated reaction solution from the reaction mechanism 40 to the magnetic separation mechanism 50;

The cleaning liquid dispensing structure of the magnetic separation mechanism 50 fills the cleaning liquid into the holding cup 103 with the reaction liquid;

The magnetic attraction structure adsorbs the reaction complex with the magnetic beads in the holding cup 103 through the magnetic field;

The liquid suction structure discharges other substances and liquids except the adsorbed reaction complexes out of the holding cup 103 .

S106: inject the substrate;

The substrate dispensing mechanism of the magnetic separation mechanism 50 injects the substrate A into the holding cup 103 after the liquid aspirating, and the substrate A luminescently labels the reaction complex in the reaction solution.

S107: ultrasonic mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution injected into the substrate A from the magnetic separation mechanism 50 to the mixing position 102;

The ultrasonic device 10 transmits ultrasonic waves to the mixing station 102 to inject the reaction solution of the substrate, and performs an ultrasonic mixing operation on the reaction solution. The ultrasonic device 10 uniformly disperses the components in the reaction solution, so that the substrate can sufficiently label the reaction complexes in the reaction solution.

When the controller 60 controls the ultrasonic device 10, the controller 60 obtains the test items input or selected by the doctor, and obtains the test item parameters corresponding to the test items, and matches an ultrasonic mode response from a plurality of ultrasonic modes according to the test item parameters. The liquid was mixed by ultrasonic.

Different ultrasonic modes have different ultrasonic mixing intensities or different ultrasonic mixing times. The mixing intensity is controlled by the input power. It can be set to include at least three ultrasonic mixing intensities: strong, medium and weak, and can be set to include at least 1s. and 2s two ultrasonic mixing times.

Ultrasonic mixing modes include at least the following:

In the first mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is medium, and the ultrasonic mixing time is 1 s;

In the second mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is weak, and the ultrasonic mixing time is 2s;

In the third mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is strong, and the ultrasonic mixing time is 1 s.

The test item parameters include numbers, letters, or a combination of the two. For example, the test item parameter of the TNI (troponin) item is 2, and the test item parameter of the E2 (estradiol) item includes 0 and 1. The controller 60 pre-stores item test parameters corresponding to different test items, and each item test parameter corresponds to a mixing mode. If the test item parameter 0 corresponds to the first mixing mode, when the test item parameter obtained by the controller 60 is 0, the ultrasonic device 10 is driven to perform ultrasonic mixing with a medium intensity and a time of 1 s on the reaction solution; test Item parameter 1 corresponds to the second mixing mode. When the test item parameter obtained by the controller 60 is 1, the ultrasonic device 10 is driven to perform ultrasonic mixing with weak intensity and 2s time on the reaction solution; the test item parameter 2 corresponds to the third mixing mode. When the parameter of the test item obtained by the controller 60 is operation 2, the ultrasonic device 10 is driven to perform ultrasonic mixing with a strong intensity and a time of 1 s on the reaction solution.

Different ultrasonic mixing modes can be set according to specific test items, so that the ultrasonic device 10 can effectively ultrasonically mix the reaction solution in different test items.

S108: incubation;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution injected with the substrate A from the mixing station 102 to the outer ring of the reaction mechanism 40 for incubation.

S109: light measurement;

The reaction mechanism 40 transfers the accommodating cup 103 of the incubated reaction solution to the detection position;

The measuring mechanism 90 detects the light-emitting reaction complex in the holding cup 103;

After completing the optical measurement, the waste liquid suction mechanism sucks the reaction liquid from the holding cup 103;

The first transfer mechanism 81 transfers the accommodating cup 103 from which the reaction liquid is discharged from the reaction mechanism 40 to the cup throwing position 72 .

In this sample analysis method, ultrasonic mixing is performed on the reaction solution after incubation (the reaction solution before magnetic separation). The ultrasonic mixing operation can separate other substances entangled in the reaction complex, thereby improving the detection accuracy.

In order to verify the effectiveness of this sample analysis method in improving the accuracy of detection, the following verification work is carried out:

Please refer to Figure 15. For the same batch of model samples, clinical tests were performed with and without ultrasonic mixing, and the final test results were compared, and compared with the standard test results of the centrifugal supernatant of the sample. It can be seen from the comparison of test results that after ultrasonic mixing, the test of the model sample is no longer closer to the real value of the sample. That is, ultrasonically mixing the reaction solution can improve the mixing effect and make the measured value more accurate.

In one embodiment, the ultrasonic mixing in step 107 is performed in the magnetic separation mechanism 50, and the specific steps are as follows:

The ultrasonic device 10 performs an ultrasonic mixing operation on the reaction solution injected into the substrate A in the magnetic separation mechanism 50 , and the second transfer mechanism 82 then transfers the holding cup 103 containing the ultrasonically injected reaction solution into the substrate A from the magnetic separation mechanism 50 . Transferred to the reaction mechanism 40 .

Ultrasonic mixing in the magnetic separation mechanism 50 can also disperse the components of the reaction solution injected into the substrate A uniformly, and the second transfer mechanism 82 can also be omitted to transfer the holding cup 103 containing the reaction solution injected into the substrate A from the The step of transferring the magnetic separation mechanism 50 to the mixing position 102 saves the transport time, thereby improving the detection efficiency.

In an embodiment, the ultrasonic mixing in step 107 is performed in the reaction mechanism 40, and the specific steps are as follows:

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution injected with the substrate A from the magnetic separation mechanism 50 to the reaction mechanism 40 , and the ultrasonic device 10 performs ultrasonic mixing on the reaction solution injected with the substrate A in the reaction mechanism 40 . operate.

Ultrasonic mixing in the reaction mechanism 40 can also disperse the components of the reaction solution injected into the substrate A uniformly, and the second transfer mechanism 82 can also be omitted to transfer the holding cup 103 containing the reaction solution injected into the substrate A from the magnetic The step of transferring to the mixing position 102 in the separation mechanism 50 saves the transfer time, thereby improving the detection efficiency.

In one embodiment, a sample analysis method is provided. The difference between the sample analysis method and the sample analysis method in the above-mentioned embodiment is that the reaction solution injected into the substrate A after incubation is ultrasonically mixed.

Please refer to FIG. 16 , the sample analysis method of this embodiment includes the following steps:

S201: Add sample;

The first transfer mechanism 81 transfers the new accommodating cup 103 on the cup-upper mechanism 71 to the sample adding position 101;

The sample dispensing mechanism 22 sucks the sample S from the sample carrying mechanism 21 , and fills the sucked sample S into the accommodating cup 103 on the sample adding position 101 .

S202: add reagent;

The first transfer mechanism 81 transfers the holding cup 103 containing the sample S from the sample adding position 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the holding cup 103 containing the reagent R to the reagent adding position;

The reagent dispensing mechanism 32 sucks the reagent R from the reagent carrying mechanism 31 , and fills the sucked reagent into the accommodating cup 103 at the reagent loading position in the reaction mechanism 40 , and the sample S and the reagent R in the accommodating cup 103 are mixed to form The reaction solution.

S203: vortex mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution to the mixing position 102;

A vortex mixing device is used to perform a vortex mixing operation on the reaction solution in the holding cup 103, so that the sample S and the reagent R can fully react.

S204: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the vortex-mixed reaction solution from the mixing position 102 back to the inner circle of the reaction mechanism 40 for incubation for a preset time.

S205: Magnetic separation;

The second transfer mechanism 82 transfers the holding cup 103 containing the incubated reaction solution from the reaction mechanism 40 to the magnetic separation mechanism 50;

The cleaning liquid dispensing structure of the magnetic separation mechanism 50 fills the cleaning liquid into the holding cup 103 with the reaction liquid;

The magnetic attraction structure adsorbs the reaction complex with the magnetic beads in the holding cup 103 through the magnetic field;

The liquid suction structure discharges other substances and liquids except the adsorbed reaction complexes out of the holding cup 103 .

S206: inject the substrate;

The substrate dispensing mechanism of the magnetic separation mechanism 50 injects the substrate A into the holding cup 103 after the liquid aspirating, and the substrate A luminescently labels the reaction complex in the reaction solution.

S207: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the reaction solution injected with the substrate A from the magnetic separation mechanism 50 back to the outer ring of the reaction mechanism 40 for incubation.

S208: ultrasonic mixing;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the incubated reaction solution injected into the substrate A from the reaction mechanism 40 to the mixing position 102;

The ultrasonic device 10 transmits ultrasonic waves into the reaction solution injected with the substrate after incubation on the mixing station 102, and performs ultrasonic mixing operation on the reaction solution. The ultrasonic device 10 uniformly disperses the aggregated substances in the reaction solution to improve the accuracy of optical measurement.

The operation of ultrasonic mixing is the same as the above-mentioned embodiment, and different ultrasonic modes are matched for different test items to effectively mix the reaction solution.

S209: light measurement;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the sonicated reaction solution injected into the substrate A from the mixing position 102 to the reaction mechanism 40;

The reaction mechanism 40 transfers the accommodating cup 103 of the incubated reaction solution to the detection position;

The measuring mechanism 90 detects the light-emitting reaction complex in the holding cup 103;

After completing the optical measurement, the waste liquid suction mechanism sucks the reaction liquid from the holding cup 103;

The first transfer mechanism 81 transfers the accommodating cup 103 from which the reaction liquid is discharged from the reaction mechanism 40 to the cup throwing position 72 .

In one embodiment, the ultrasonic mixing in step 208 is performed in the reaction mechanism 40, and the specific steps are as follows:

The ultrasonic device 10 performs an ultrasonic mixing operation on the reaction solution injected into the substrate A after incubation in the reaction mechanism 40 .

Ultrasonic mixing in the reaction mechanism 40 can also uniformly disperse the aggregated substances in the reaction solution injected into the substrate A after incubation, and the second transfer mechanism 82 can also be omitted. The two transfer steps of the accommodating cup 103 between the reaction mechanism 40 and the mixing position 102 save the transfer time, thereby improving the detection efficiency.

In one embodiment, a sample analysis method is provided. The difference between the sample analysis method and the sample analysis method in the above-mentioned embodiment is that the reaction solution injected into the substrate A to be incubated and after incubation is subjected to ultrasonic mixing.

Please refer to FIG. 17 , the sample analysis method of this embodiment includes the following steps:

S301: Add sample;

The first transfer mechanism 81 transfers the new accommodating cup 103 on the cup-upper mechanism 71 to the sample adding position 101;

The sample dispensing mechanism 22 sucks the sample S from the sample carrying mechanism 21 , and fills the sucked sample S into the accommodating cup 103 on the sample adding position 101 .

S302: add reagent;

The first transfer mechanism 81 transfers the holding cup 103 containing the sample S from the sample adding position 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the holding cup 103 containing the reagent R to the reagent adding position;

The reagent dispensing mechanism 32 sucks the reagent R from the reagent carrying mechanism 31 , and fills the sucked reagent into the accommodating cup 103 at the reagent loading position in the reaction mechanism 40 , and the sample S and the reagent R in the accommodating cup 103 are mixed to form The reaction solution.

S303: vortex mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution to the mixing position 102;

A vortex mixing device is used to perform a vortex mixing operation on the reaction solution in the holding cup 103, so that the sample S and the reagent R can fully react.

S304: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the vortex-mixed reaction solution from the mixing position 102 back to the inner circle of the reaction mechanism 40 for incubation for a preset time.

S305: Magnetic separation;

The second transfer mechanism 82 transfers the holding cup 103 containing the incubated reaction solution from the reaction mechanism 40 to the magnetic separation mechanism 50;

The cleaning liquid dispensing structure of the magnetic separation mechanism 50 fills the cleaning liquid into the holding cup 103 with the reaction liquid;

The magnetic attraction structure adsorbs the reaction complex with the magnetic beads in the holding cup 103 through the magnetic field;

The liquid suction structure discharges other substances and liquids except the adsorbed reaction complexes out of the holding cup 103 .

S306: inject the substrate;

The substrate dispensing mechanism of the magnetic separation mechanism 50 injects the substrate A into the holding cup 103 after the liquid aspirating, and the substrate A luminescently labels the reaction complex in the reaction solution.

S307: ultrasonic mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution injected into the substrate A from the reaction mechanism 40 to the mixing position 102;

The ultrasonic device 10 transmits ultrasonic waves to the mixing station 102 to inject the reaction solution of the substrate, and performs an ultrasonic mixing operation on the reaction solution. The ultrasonic device 10 uniformly disperses the aggregated substances in the reaction solution to improve the accuracy of optical measurement.

The operation of ultrasonic mixing is the same as the above-mentioned embodiment, and different ultrasonic modes are matched for different test items to effectively mix the reaction solution.

S308: incubation;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution injected into the substrate A from the mixing station 102 back to the outer ring of the reaction mechanism 40 for incubation.

S309: ultrasonic mixing;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the incubated reaction solution injected into the substrate A from the reaction mechanism 40 to the mixing position 102;

The ultrasonic device 10 transmits ultrasonic waves into the reaction solution injected with the substrate after incubation on the mixing station 102, and performs ultrasonic mixing operation on the reaction solution. The ultrasonic device 10 uniformly disperses the aggregated substances in the reaction solution to improve the accuracy of optical measurement.

The operation of ultrasonic mixing is the same as the above-mentioned embodiment, and different ultrasonic modes are matched for different test items to effectively mix the reaction solution.

S310: photometric;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the sonicated reaction solution injected into the substrate A from the mixing position 102 to the reaction mechanism 40;

The reaction mechanism 40 transfers the accommodating cup 103 of the incubated reaction solution to the detection position;

The measuring mechanism 90 detects the light-emitting reaction complex in the holding cup 103;

After completing the optical measurement, the waste liquid suction mechanism sucks the reaction liquid from the holding cup 103;

The first transfer mechanism 81 transfers the accommodating cup 103 from which the reaction liquid is discharged from the reaction mechanism 40 to the cup throwing position 72 .

In one embodiment, the ultrasonic mixing in step 307 is performed in the magnetic separation mechanism 50 , and the ultrasonic mixing in step 309 is still performed in the ultrasonic position 102 . The specific steps of performing ultrasonic mixing in the magnetic separation mechanism 50 are as follows:

The ultrasonic device 10 performs an ultrasonic mixing operation on the reaction solution injected into the substrate A in the magnetic separation mechanism 50 , and the second transfer mechanism 82 then transfers the holding cup 103 containing the ultrasonically injected reaction solution into the substrate A from the magnetic separation mechanism 50 . Transferred to the reaction mechanism 40 .

Ultrasonic mixing in the magnetic separation mechanism 50 can also disperse the components of the reaction solution injected into the substrate A uniformly, and the second transfer mechanism 82 can also be omitted to transfer the holding cup 103 containing the reaction solution injected into the substrate A from the The step of transferring the magnetic separation mechanism 50 to the mixing position 102 saves the transport time, thereby improving the detection efficiency.

In one embodiment, the ultrasonic mixing in step 307 is performed in the reaction mechanism 40 , and the ultrasonic mixing in step 309 is still performed in the ultrasonic position 102 . The specific steps of performing ultrasonic mixing in the reaction mechanism 40 are as follows:

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution injected with the substrate A from the magnetic separation mechanism 50 to the reaction mechanism 40 , and the ultrasonic device 10 performs ultrasonic mixing on the reaction solution injected with the substrate A in the reaction mechanism 40 . operate.

Ultrasonic mixing in the reaction mechanism 40 can also disperse the components of the reaction solution injected into the substrate A uniformly, and the second transfer mechanism 82 can also be omitted to transfer the holding cup 103 containing the reaction solution injected into the substrate A from the magnetic The step of transferring to the mixing position 102 in the separation mechanism 50 saves the transfer time, thereby improving the detection efficiency.

In one embodiment, the ultrasonic mixing in step 307 and the ultrasonic mixing in step 309 are performed in the reaction mechanism 40 . The specific steps of performing ultrasonic mixing in the reaction mechanism 40 are as follows:

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution injected with the substrate A from the magnetic separation mechanism 50 to the reaction mechanism 40 . Ultrasonic mixing operation;

After the incubation is completed, the ultrasonic device 10 performs an ultrasonic mixing operation on the reaction solution injected into the substrate A after incubation in the reaction mechanism 40 .

Ultrasonic mixing in the reaction mechanism 40 can also uniformly disperse the aggregated substances in the reaction solution injected into the substrate A to be incubated and incubated, and the second transfer mechanism 82 can also be omitted. The accommodating cup 103 of the reaction solution is transferred between the reaction mechanism 40 and the mixing position 102, which saves the transfer time and improves the detection efficiency.

The above application of specific examples to illustrate the present invention is only used to help the understanding of the present invention, and is not intended to limit the present invention. For those skilled in the art to which the present invention pertains, according to the idea of the present invention, several simple deductions, modifications or substitutions can also be made.

Claims (18)

1. A sample analyzer, comprising:

a substrate dispensing structure for dispensing a substrate into the reaction solution in the receiving cup;

a measuring mechanism for measuring the reaction solution;

ultrasonic means for generating ultrasonic vibration to form ultrasonic waves; and

and the controller is connected with the ultrasonic device and is used for controlling the ultrasonic device to emit ultrasonic waves into the reaction liquid, filled with the substrate, in the containing cup.

2. The sample analyzer of claim 1, wherein the controller is configured to obtain test item parameters and perform an ultrasonic mixing operation on the reaction solution injected with the substrate according to the test item parameters by matching one of a plurality of preset ultrasonic modes.

3. The sample analyzer of claim 2 wherein the plurality of ultrasound modes each have a different ultrasound intensity and/or ultrasound exposure time.

4. The sample analyzer of claim 1 wherein the ultrasonic device comprises an ultrasonic transducer for generating ultrasonic vibrations, a transmission member having a first end and a second end, the first end of the transmission member being coupled to the ultrasonic transducer, the second end of the transmission member having an outer diameter less than an inner diameter of the containment cup; the moving device is connected with the ultrasonic transducer, the moving device is used for driving the ultrasonic transducer and the transmission piece to move relative to the containing cup, and the second end of the transmission piece can be inserted into the reaction liquid of the injected substrate in the containing cup so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the reaction liquid of the injected substrate in the containing cup.

5. The sample analyzer of claim 1 wherein the ultrasonic device comprises an ultrasonic transducer for generating ultrasonic vibrations and a transmission member having a first end and a second end, the first end of the transmission member being connected to the ultrasonic transducer; the second end of the transmission piece is used for abutting against the outer wall of the containing cup, and the part of the outer wall of the containing cup, which is contacted with the transmission piece, is a part surrounding the reaction liquid filled with the substrate, so that the ultrasonic vibration generated by the ultrasonic transducer is transmitted to the reaction liquid filled with the substrate in the containing cup.

6. The sample analyzer of claim 4 or 5 wherein the transmission member is a solid structure, and the outer diameter of the transmission member decreases gradually or in steps from the first end to the second end.

7. The sample analyzer of any one of claims 1 to 5, further comprising a reaction mechanism for providing an incubation location for the reaction solution injected with the substrate in the receiving cup, and the controller is configured to control the ultrasonic device to perform an ultrasonic mixing operation on the reaction solution injected with the substrate to be incubated and/or the reaction solution injected with the substrate after incubation.

8. The sample analyzer of claim 7, wherein the controller is configured to control the ultrasonic device to perform an ultrasonic mixing operation on the substrate-injected reaction solution to be incubated and/or the substrate-injected reaction solution after incubation in the reaction mechanism.

9. The sample analyzer of claim 7, further comprising a magnetic separation mechanism for performing a magnetic separation operation on the reaction solution, and a substrate dispensing mechanism for injecting a substrate into the magnetically separated reaction solution on the magnetic separation mechanism, and a transfer mechanism for transferring the receiving cup between the magnetic separation mechanism and the reaction mechanism;

the controller is used for controlling the ultrasonic device to perform ultrasonic mixing operation on the reaction liquid which is to be incubated and is injected with the substrate on the magnetic separation mechanism and/or the reaction liquid which is incubated and is injected with the substrate on the reaction mechanism.

10. The sample analyzer of claim 7, further comprising a magnetic separation mechanism and a transfer mechanism, wherein the magnetic separation mechanism is configured to perform a magnetic separation operation on the reaction solution, the substrate dispensing mechanism is configured to inject a substrate into the reaction solution after the magnetic separation on the magnetic separation mechanism, a mixing position is provided outside the reaction mechanism and the magnetic separation mechanism, and the transfer mechanism is configured to transfer the receiving cup among the reaction mechanism, the magnetic separation mechanism, and the mixing position;

the ultrasonic device is used for carrying out ultrasonic blending operation on the reaction liquid to be incubated and injected with the substrate and/or the reaction liquid to be incubated and injected with the substrate at the blending position.

11. A method of analyzing a sample, comprising the steps of:

a sample dispensing mechanism and a reagent dispensing mechanism respectively inject a sample and a reagent into the containing cup to form a reaction liquid;

incubating the reaction solution in the reaction mechanism;

the magnetic separation mechanism performs magnetic separation operation on the incubated reaction liquid;

the substrate separate injection structure injects the substrate into the reaction liquid after magnetic separation in the containing cup;

incubating the reaction solution injected with the substrate in the reaction mechanism;

the ultrasonic device emits ultrasonic waves into the reaction liquid in which the substrate is injected;

the measuring means optically measures the reaction solution.

12. The sample analysis method according to claim 11, wherein the ultrasonic kneading operation of the ultrasonic device is controlled by:

acquiring test item parameters;

and according to the test items, matching one ultrasonic mode from a plurality of preset ultrasonic modes by the parameters to perform ultrasonic blending operation on the reaction liquid injected with the substrate.

13. The sample analysis method as claimed in claim 12, wherein the plurality of ultrasound modes have different ultrasound intensities and/or ultrasound durations, respectively.

14. The method for analyzing a sample according to claim 11, wherein the ultrasonic device performs an ultrasonic mixing operation on the reaction solution to be incubated into which the substrate is injected and/or the reaction solution to be incubated into which the substrate is injected.

15. The method for analyzing a sample according to claim 14, wherein the ultrasonic device performs an ultrasonic mixing operation on the substrate-injected reaction solution to be incubated and/or the substrate-injected reaction solution after incubation in the reaction mechanism.

16. The method for analyzing a sample according to claim 14, wherein the ultrasonic device performs an ultrasonic mixing operation on the reaction solution to be incubated into which the substrate is injected on the magnetic separation means and/or on the reaction means after the incubation.

17. The method for analyzing a sample according to claim 14, wherein the step of injecting the substrate into the reaction solution after the magnetic separation in the receiving cup by the substrate dispensing structure and the step of incubating the reaction solution into which the substrate is injected in the reaction mechanism further comprises the steps of:

the transfer mechanism transfers the containing cup filled with the reaction liquid into which the substrate is injected from the magnetic separation mechanism to a mixing position;

the ultrasonic device carries out ultrasonic blending operation on the reaction liquid injected with the substrate on the blending position;

the transfer mechanism transfers the containing cup containing the reaction solution which is injected with the substrate and is subjected to ultrasonic treatment to the reaction mechanism from the mixing position.

18. The method for analyzing a sample according to claim 14 or 17, wherein the step of injecting the reaction solution into which the substrate is injected is performed between the step of incubating the reaction solution in the reaction means and the step of optically measuring the reaction solution by the measuring means, further comprising the step of:

the transfer mechanism transfers the holding cup filled with the incubated reaction liquid injected with the substrate from the reaction mechanism to a mixing position;

the ultrasonic device carries out ultrasonic mixing operation on the incubated reaction liquid injected with the substrate on the mixing position;

the transfer mechanism transfers the containing cup filled with the reaction liquid injected with the substrate after the ultrasonic treatment from the mixing position to the reaction mechanism.

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