CN114525177A - A kind of enzymatic deacidification method of high acid value oil and fat - Google Patents
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- 239000002253 acid Substances 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 18
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 69
- 230000005496 eutectics Effects 0.000 claims abstract description 33
- 239000002904 solvent Substances 0.000 claims abstract description 31
- 235000021588 free fatty acids Nutrition 0.000 claims abstract description 16
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims abstract description 14
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims abstract description 14
- 229960003237 betaine Drugs 0.000 claims abstract description 14
- 238000005886 esterification reaction Methods 0.000 claims abstract description 14
- 108090001060 Lipase Proteins 0.000 claims abstract description 13
- 239000004367 Lipase Substances 0.000 claims abstract description 13
- 102000004882 Lipase Human genes 0.000 claims abstract description 13
- 235000019421 lipase Nutrition 0.000 claims abstract description 13
- 239000004519 grease Substances 0.000 claims abstract 4
- 239000003921 oil Substances 0.000 claims description 56
- 238000006243 chemical reaction Methods 0.000 claims description 25
- 239000003925 fat Substances 0.000 claims description 14
- 235000014593 oils and fats Nutrition 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 8
- 108010048733 Lipozyme Proteins 0.000 claims description 7
- FCCDDURTIIUXBY-UHFFFAOYSA-N lipoamide Chemical compound NC(=O)CCCCC1CCSS1 FCCDDURTIIUXBY-UHFFFAOYSA-N 0.000 claims description 7
- 108010084311 Novozyme 435 Proteins 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 2
- 230000032050 esterification Effects 0.000 abstract description 5
- 125000005456 glyceride group Chemical group 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 description 49
- 230000003247 decreasing effect Effects 0.000 description 9
- 235000019774 Rice Bran oil Nutrition 0.000 description 8
- 239000008165 rice bran oil Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 239000011541 reaction mixture Substances 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 102100021851 Calbindin Human genes 0.000 description 1
- 101000898082 Homo sapiens Calbindin Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 101001021643 Pseudozyma antarctica Lipase B Proteins 0.000 description 1
- 238000009874 alkali refining Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010497 wheat germ oil Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
- C11C3/04—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils
- C11C3/06—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils with glycerol
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/74—Recovery of fats, fatty oils, fatty acids or other fatty substances, e.g. lanolin or waxes
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
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Abstract
Description
技术领域technical field
本发明属于油脂加工技术领域，具体涉及一种高酸价油脂的酶法脱酸方法。The invention belongs to the technical field of oil and fat processing, and in particular relates to an enzymatic deacidification method for high-acid-value fats and oils.
背景技术Background technique
高酸价油脂一般是指酸价大于10mg KOH/g的油脂。油脂的高酸价给其储存、加工和利用带来了巨大的困难。目前，常见的高酸价油脂主要有：高酸价米糠油、高酸价小麦胚芽油、高酸价鱼油、高酸价餐饮废油、酸化油和脱臭馏出物等。对高酸价油脂进行脱酸再利用，不仅可实现资源的高值化利用，而且有利于环境保护。High acid value oils generally refer to oils and fats with an acid value greater than 10 mg KOH/g. The high acid value of oil brings great difficulties to its storage, processing and utilization. At present, common high-acid-value oils and fats mainly include: high-acid-value rice bran oil, high-acid-value wheat germ oil, high-acid-value fish oil, high-acid-value food waste oil, acidified oil, and deodorized distillate. Deacidification and reuse of high-acid-valued oils and fats can not only achieve high-value utilization of resources, but also be beneficial to environmental protection.
传统的化学碱炼会生成大量的皂，从而造成中性油的大量损失，因此并不适用于高酸价油脂的脱酸；物理蒸馏脱酸尽管脱酸效果好，但存在能耗高、对原料含磷量要求高等问题；溶剂萃取和膜分离脱酸是近年来发展起来的新型脱酸技术，但存在脱酸效率低、效果差和应用成本高等问题。相比于上述几种脱酸方法，酶法脱酸具有反应条件温和、催化特异性高、脱酸效果好及安全环保等优点，是近年来油脂脱酸领域的主要发展方向。赵晨伟等(米糠毛油酶法脱酸的工艺优化，中国油脂，2019，44(4)：17-20)采用固定化脂肪酶CALB在高真空条件下(<1000Pa)催化高酸价米糠油脱酸，反应6h，高酸价米糠油的酸价由20.52mgKOH/g降至1.54mg KOH/g；万聪等(无溶剂体系高酸值米糠油酶法酯化脱酸工艺优化研究，2016，41(4)：10-13)采用Lipozyme 435在0.09MPa的条件下催化高酸价米糠油脱酸，反应10h，高酸价米糠油的酸价由39.81mg KOH/g降至2.06mg KOH/g；Li等(Journal ofAgricultural and Food Chemistry,2016,64(35):6730-6737)采用固定化偏高甘油酯脂肪酶SMG1-F278N在正己烷体系下通过分批添加乙醇的方式催化高酸价米糠油脱酸，反应6h，高酸价米糠油的酸价由50.12mg KOH/g降至0.10mg KOH/g。综上可知，现有酶法脱酸技术反应条件温和、脱酸效果好，但脱酸过程中仍需借助高真空或有机溶剂等来提高脱酸效率和效果，脱酸时间仍较长。The traditional chemical alkali refining will generate a large amount of soap, resulting in a large loss of neutral oil, so it is not suitable for the deacidification of high acid value oils; although the deacidification effect of physical distillation is good, it has high energy consumption and is not suitable for deacidification. The phosphorus content of raw materials is high; solvent extraction and membrane separation deacidification are new deacidification technologies developed in recent years, but there are problems such as low deacidification efficiency, poor effect and high application cost. Compared with the above several deacidification methods, enzymatic deacidification has the advantages of mild reaction conditions, high catalytic specificity, good deacidification effect, safety and environmental protection, etc., and is the main development direction in the field of oil deacidification in recent years. Zhao Chenwei et al. (Process optimization of enzymatic deacidification of crude rice bran oil, China Oil & Oil, 2019, 44(4): 17-20) Using immobilized lipase CALB to catalyze the deacidification of high acid value rice bran oil under high vacuum conditions (<1000Pa) acid, reacted for 6h, the acid value of high acid value rice bran oil decreased from 20.52mgKOH/g to 1.54mg KOH/g; Wan Cong et al. 41(4): 10-13) Using Lipozyme 435 to catalyze the deacidification of high-acid value rice bran oil under the condition of 0.09MPa, the reaction 10h, the acid value of high-acid value rice bran oil decreased from 39.81mg KOH/g to 2.06mg KOH/ g; Li et al. (Journal of Agricultural and Food Chemistry, 2016, 64(35): 6730-6737) used immobilized high glyceride lipase SMG1-F278N to catalyze high acid value by adding ethanol in batches under n-hexane system The rice bran oil was deacidified and reacted for 6 hours, and the acid value of the high-acid value rice bran oil decreased from 50.12 mg KOH/g to 0.10 mg KOH/g. To sum up, the existing enzymatic deacidification technology has mild reaction conditions and good deacidification effect, but still needs to use high vacuum or organic solvent to improve the deacidification efficiency and effect in the deacidification process, and the deacidification time is still long.
发明内容SUMMARY OF THE INVENTION
为了解决上述现有技术中存在的缺陷，本发明的目的在于提供一种高酸价油脂的酶法脱酸方法，能够大幅提升脱酸效率，实现高酸价油脂的快速脱酸。In order to solve the above-mentioned defects in the prior art, the purpose of the present invention is to provide an enzymatic deacidification method for high-acid-value oils and fats, which can greatly improve the deacidification efficiency and realize rapid deacidification of high-acid-value oils and fats.
本发明是通过以下技术方案来实现：The present invention is achieved through the following technical solutions:
一种高酸价油脂的酶法脱酸方法，包括以下步骤：A kind of enzymatic deacidification method of high acid value oil, comprises the following steps:
步骤1：在甜菜碱与甘油组成的低共熔溶剂中，采用固定化脂肪酶在预设转速下催化高酸价油脂进行酯化反应；Step 1: in a low eutectic solvent composed of betaine and glycerol, using immobilized lipase to catalyze the esterification reaction of high-acid oil at a preset rotational speed;
步骤2：反应结束后进行离心处理，得到脱酸后的油脂。Step 2: After the reaction is completed, centrifugation is performed to obtain deacidified oil.
优选地，步骤1中，甜菜碱与甘油的摩尔比为1：4～8。Preferably, in step 1, the molar ratio of betaine to glycerol is 1:4-8.
优选地，步骤1中，甘油与高酸价油脂中的游离脂肪酸的摩尔比为2～6：1。Preferably, in step 1, the molar ratio of glycerol to the free fatty acid in the high-acid oil and fat is 2-6:1.
优选地，步骤1中，固定化脂肪酶为Novozym 435或Lipozyme 435。Preferably, in step 1, the immobilized lipase is Novozym 435 or Lipozyme 435.
进一步优选地，固定化脂肪酶的添加量为底物总质量的2％～6％。Further preferably, the added amount of the immobilized lipase is 2% to 6% of the total mass of the substrate.
优选地，步骤1中，酯化反应的温度为40～70℃，酯化反应的时间为0.5～2h。Preferably, in step 1, the temperature of the esterification reaction is 40-70° C., and the time of the esterification reaction is 0.5-2 h.
优选地，步骤1中，预设转速为200～500rpm。Preferably, in step 1, the preset rotational speed is 200-500 rpm.
优选地，步骤2中，离心的转速为8000～15000rpm，离心的时间为1～2min。Preferably, in step 2, the rotation speed of centrifugation is 8000-15000 rpm, and the time of centrifugation is 1-2 min.
与现有技术相比，本发明具有以下有益的技术效果：Compared with the prior art, the present invention has the following beneficial technical effects:
本发明公开的高酸价油脂的酶法脱酸方法，在甜菜碱与甘油组成的低共熔溶剂中进行，由于甜菜碱与甘油组成的低共熔溶剂对游离脂肪酸具有较好的溶解度，因此可高效萃取高酸价油脂中的游离脂肪酸；此外，低共熔溶剂中脂肪酶催化的酯化反应也能将高酸价油脂中的游离脂肪酸转化生成甘油酯，并且酯化反应过程中生成的水分可被甜菜碱与甘油组成的低共熔溶剂通过形成氢键所捕获，从而推动游离脂肪酸向甘油酯转化。在低共熔溶剂萃取与低共熔溶剂中脂肪酶催化的酯化反应的共同作用下，高酸价油脂的酸价迅速降低。该方法脱酸速率快、效果好、产物分离简单且绿色环保，具有良好的经济、生态效益和工业应用前景。The enzymatic deacidification method of the high acid value fat and oil disclosed in the invention is carried out in a deep eutectic solvent composed of betaine and glycerol. It can efficiently extract free fatty acids in high-acid oils; in addition, the esterification reaction catalyzed by lipase in a deep eutectic solvent can also convert free fatty acids in high-acid oils to glycerides. Moisture can be captured by the deep eutectic solvent of betaine and glycerol by forming hydrogen bonds, thereby promoting the conversion of free fatty acids to glycerides. Under the combined action of deep eutectic solvent extraction and lipase-catalyzed esterification in deep eutectic solvent, the acid value of high-acid-valued oils and fats decreases rapidly. The method has fast deacidification rate, good effect, simple product separation and environmental protection, and has good economic, ecological benefits and industrial application prospects.
进一步地，甜菜碱与甘油的摩尔比为1：4～8，不仅有利于低共熔溶剂萃取游离脂肪酸，而且有利于脂肪酶在低共熔溶剂中将游离脂肪酸高效转化生成甘油酯。Further, the molar ratio of betaine to glycerol is 1:4-8, which not only facilitates the extraction of free fatty acids by the deep eutectic solvent, but also facilitates the efficient conversion of free fatty acids by lipase into glycerides in the deep eutectic solvent.
进一步地，甘油与高酸价油脂中的游离脂肪酸的摩尔比为2～6：1，可确保较高的脂肪酸酯化率。Further, the molar ratio of glycerol to free fatty acids in high-acid-value fats and oils is 2 to 6:1, which can ensure a high fatty acid esterification rate.
进一步地，固定化脂肪酶采用Novozym 435或Lipozyme 435，能够确保较高的脂肪酸选择性和酯化率。Further, Novozym 435 or Lipozyme 435 was used as the immobilized lipase, which could ensure higher fatty acid selectivity and esterification rate.
进一步地，酯化反应的温度为40～70℃，不仅能够确保低共熔溶剂对脂肪酸具有较高的萃取效率，而且能够确保脂肪酶具有较高的反应活性和稳定性。Further, the temperature of the esterification reaction is 40-70° C., which can not only ensure that the deep eutectic solvent has high extraction efficiency for fatty acids, but also can ensure that the lipase has high reactivity and stability.
进一步地，离心的转速为8000～15000rpm，离心的时间为1～2min，能够使产物快速、高回收率的回收。Further, the rotational speed of the centrifugation is 8000-15000 rpm, and the time of the centrifugation is 1-2 min, which can enable the product to be recovered quickly and with a high recovery rate.
具体实施方式Detailed ways
下面结合具体的实施例对本发明做进一步的详细说明，所述是对本发明的解释而不是限定。如非写明，所有百分比均为质量百分比。The present invention will be further described in detail below in conjunction with specific embodiments, which are to explain rather than limit the present invention. All percentages are by mass unless otherwise specified.
实施例1Example 1
在5L的反应瓶中加入2Kg的低共熔溶剂和高酸价油脂(56.48mg KOH/g)的混合物，其中低共熔溶剂由甜菜碱和甘油组成，二者的摩尔比为1：4，低共熔溶剂中的甘油与高酸价油脂中的游离脂肪酸的摩尔比为2：1。将反应混合物预热到40℃后，加入40g的Novozym435，反应在200rpm的搅拌速度下进行。反应2h后，在15000rpm的转速下离心1min分离得到脱酸后的油脂。分析脱酸后油脂的酸价，发现脱酸后油脂的酸价降至0.46mg KOH/g。In the 5L reaction flask, add the mixture of 2Kg of deep eutectic solvent and high acid value oil (56.48mg KOH/g), wherein the deep eutectic solvent is made up of betaine and glycerol, and the mol ratio of the two is 1:4, The molar ratio of the glycerol in the deep eutectic solvent to the free fatty acid in the high-acid oil and fat is 2:1. After the reaction mixture was preheated to 40°C, 40 g of Novozym 435 was added and the reaction was carried out at a stirring speed of 200 rpm. After 2 hours of reaction, centrifuge at 15,000 rpm for 1 min to obtain deacidified oil. The acid value of the oil after deacidification was analyzed, and it was found that the acid value of the oil after deacidification decreased to 0.46 mg KOH/g.
实施例2Example 2
在5L的反应瓶中加入2Kg的低共熔溶剂和高酸价油脂(56.48mg KOH/g)的混合物，其中低共熔溶剂由甜菜碱和甘油组成，二者的摩尔比为1：6，低共熔溶剂中的甘油与高酸价油脂中的游离脂肪酸的摩尔比为4：1。将反应混合物预热到60℃后，加入120g的Novozym435，反应在300rpm的搅拌速度下进行。反应0.5h后，在8000rpm的转速下离心2min分离得到脱酸后的油脂。分析脱酸后油脂的酸价，发现脱酸后油脂的酸价降至0.10mg KOH/g。In the 5L reaction flask, add the mixture of 2Kg of deep eutectic solvent and high acid value oil (56.48mg KOH/g), wherein the deep eutectic solvent is made up of betaine and glycerol, and the mol ratio of the two is 1:6, The molar ratio of the glycerol in the deep eutectic solvent to the free fatty acid in the high-acid oil and fat is 4:1. After the reaction mixture was preheated to 60°C, 120 g of Novozym 435 was added and the reaction was carried out at a stirring speed of 300 rpm. After the reaction for 0.5h, centrifuge at 8000rpm for 2min to obtain the deacidified oil. The acid value of the oil after deacidification was analyzed, and it was found that the acid value of the oil after deacidification decreased to 0.10 mg KOH/g.
实施例3Example 3
在5L的反应瓶中加入2Kg的低共熔溶剂和高酸价油脂(56.48mg KOH/g)的混合物，其中低共熔溶剂由甜菜碱和甘油组成，二者的摩尔比为1：8，低共熔溶剂中的甘油与高酸价油脂中的游离脂肪酸的摩尔比为6：1。将反应混合物预热到70℃后，加入100g的Lipozyme435，反应在500rpm的搅拌速度下进行。反应0.5h后，在12000rpm的转速下离心1.5min分离得到脱酸后的油脂。分析脱酸后油脂的酸价，发现脱酸后油脂的酸价降至0.10mg KOH/g。In the 5L reaction flask, add the mixture of 2Kg of deep eutectic solvent and high acid value oil (56.48mg KOH/g), wherein the deep eutectic solvent is made up of betaine and glycerol, and the mol ratio of the two is 1:8, The molar ratio of the glycerol in the deep eutectic solvent to the free fatty acid in the high-acid oil and fat is 6:1. After the reaction mixture was preheated to 70°C, 100 g of Lipozyme 435 was added, and the reaction was carried out at a stirring speed of 500 rpm. After the reaction for 0.5h, centrifuge at 12000rpm for 1.5min to separate the oil after deacidification. The acid value of the oil after deacidification was analyzed, and it was found that the acid value of the oil after deacidification decreased to 0.10 mg KOH/g.
实施例4Example 4
在5L的反应瓶中加入2Kg的低共熔溶剂和高酸价油脂(56.48mg KOH/g)的混合物，其中低共熔溶剂由甜菜碱和甘油组成，二者的摩尔比为1：5，低共熔溶剂中的甘油与高酸价油脂中的游离脂肪酸的摩尔比为5：1。将反应混合物预热到50℃后，加入60g的Lipozyme435，反应在400rpm的搅拌速度下进行。反应1h后，在15000rpm的转速下离心1min分离得到脱酸后的油脂。分析脱酸后油脂的酸价，发现脱酸后油脂的酸价降至0.13mg KOH/g。In the 5L reaction flask, add the mixture of 2Kg of deep eutectic solvent and high acid value oil (56.48mg KOH/g), wherein the deep eutectic solvent is made up of betaine and glycerol, and the mol ratio of the two is 1:5, The molar ratio of the glycerol in the deep eutectic solvent to the free fatty acid in the high-acid oil and fat is 5:1. After the reaction mixture was preheated to 50°C, 60 g of Lipozyme 435 was added and the reaction was carried out at a stirring speed of 400 rpm. After 1 hour of reaction, centrifuge at 15,000 rpm for 1 min to obtain deacidified oil. The acid value of the oil after deacidification was analyzed, and it was found that the acid value of the oil after deacidification decreased to 0.13 mg KOH/g.
实施例5Example 5
在5L的反应瓶中加入2Kg的低共熔溶剂和高酸价油脂(56.48mg KOH/g)的混合物，其中低共熔溶剂由甜菜碱和甘油组成，二者的摩尔比为1：4，低共熔溶剂中的甘油与高酸价油脂中的游离脂肪酸的摩尔比为3：1，将反应混合物预热到45℃后，加入50g的Lipozyme435，反应在500rpm的搅拌速度下进行。反应1h后，在15000rpm的转速下离心1min分离得到脱酸后的油脂。分析脱酸后油脂的酸价，发现脱酸后油脂的酸价降至0.48mg KOH/g。In the 5L reaction flask, add the mixture of 2Kg of deep eutectic solvent and high acid value oil (56.48mg KOH/g), wherein the deep eutectic solvent is made up of betaine and glycerol, and the mol ratio of the two is 1:4, The molar ratio of glycerol in the deep eutectic solvent to the free fatty acid in the high-acid oil was 3:1. After the reaction mixture was preheated to 45 °C, 50 g of Lipozyme 435 was added, and the reaction was carried out at a stirring speed of 500 rpm. After 1 hour of reaction, centrifuge at 15,000 rpm for 1 min to obtain deacidified oil. The acid value of the oil after deacidification was analyzed, and it was found that the acid value of the oil after deacidification decreased to 0.48 mg KOH/g.
实施例6Example 6
在5L的反应瓶中加入2Kg的低共熔溶剂和高酸价油脂(56.48mg KOH/g)的混合物，其中低共熔溶剂由甜菜碱和甘油组成，二者的摩尔比为1：7，低共熔溶剂中的甘油与高酸价油脂中的游离脂肪酸的摩尔比为6：1，将反应混合物预热到65℃后，加入60g的Novozym435，反应在300rpm的搅拌速度下进行。反应1h后，在8000rpm的转速下离心2min分离得到脱酸后的油脂。分析脱酸后油脂的酸价，发现脱酸后油脂的酸价降至0.11mg KOH/g。In the 5L reaction flask, add the mixture of 2Kg of deep eutectic solvent and high acid value oil (56.48mg KOH/g), wherein the deep eutectic solvent is made up of betaine and glycerol, and the mol ratio of the two is 1:7, The molar ratio of glycerol in the deep eutectic solvent to the free fatty acid in the high-acid oil was 6:1. After preheating the reaction mixture to 65°C, 60 g of Novozym435 was added, and the reaction was carried out at a stirring speed of 300 rpm. After 1 h of reaction, centrifuge at 8000 rpm for 2 min to obtain deacidified oil. The acid value of the oil after deacidification was analyzed, and it was found that the acid value of the oil after deacidification decreased to 0.11 mg KOH/g.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115261148A (en) * | 2022-08-29 | 2022-11-01 | 广东省农业科学院蚕业与农产品加工研究所 | Application of deep eutectic solvent in oil dehydration |
| CN115677757A (en) * | 2022-11-21 | 2023-02-03 | 江苏极易新材料有限公司 | Method for purifying tris (2,4-di-tert-butylphenyl) phosphite ester with low chloride ion content |
| CN116042322A (en) * | 2022-11-29 | 2023-05-02 | 济宁康维生物油脂有限公司 | A method for simultaneous preparation of high-acid oil deacidification and diglyceride |
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| CN106906194A (en) * | 2017-03-09 | 2017-06-30 | 华南理工大学 | A kind of enzyme process acid stripping method of partial glyceride lipase and the grease rich in PUFA |
| CN113061486A (en) * | 2021-04-09 | 2021-07-02 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method for enzyme-catalyzed removal of free fatty acid in oil |
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| US20080305531A1 (en) * | 2004-09-10 | 2008-12-11 | Verenium Corporation | Compositions and Methods for Making and Modifying Oils |
| CN106906194A (en) * | 2017-03-09 | 2017-06-30 | 华南理工大学 | A kind of enzyme process acid stripping method of partial glyceride lipase and the grease rich in PUFA |
| CN113061486A (en) * | 2021-04-09 | 2021-07-02 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method for enzyme-catalyzed removal of free fatty acid in oil |
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| CN115261148A (en) * | 2022-08-29 | 2022-11-01 | 广东省农业科学院蚕业与农产品加工研究所 | Application of deep eutectic solvent in oil dehydration |
| CN115261148B (en) * | 2022-08-29 | 2023-06-02 | 广东省农业科学院蚕业与农产品加工研究所 | Application of Deep Eutectic Solvent in Oil Dehydration |
| CN115677757A (en) * | 2022-11-21 | 2023-02-03 | 江苏极易新材料有限公司 | Method for purifying tris (2,4-di-tert-butylphenyl) phosphite ester with low chloride ion content |
| CN116042322A (en) * | 2022-11-29 | 2023-05-02 | 济宁康维生物油脂有限公司 | A method for simultaneous preparation of high-acid oil deacidification and diglyceride |
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