CN114917967A - A rapid detection microfluidic chip - Google Patents

Abstract

The invention discloses a microfluidic chip for rapid detection, which relates to the technical field of detection equipment and includes a chip body; the chip body includes a lower-layer chip, a middle-layer chip and an upper-layer chip in sequence from bottom to top; The liquid filling port is arranged on the surface of the upper chip, and the liquid filling port is arranged in multiple groups, and each of the liquid filling ports is respectively connected with the microfluidic structure; the microfluidic structure is arranged on a side of the middle chip facing the upper chip. The microfluidic chip of the present invention is provided with a heat insulation groove structure, which can realize rapid temperature rise and fall, thereby greatly shortening the PCR reaction time, The PCR amplification process that usually takes more than minutes can be shortened to within minutes, and the microfluidic chip of the present invention can realize high-precision integration through silicon-based microfluidic chip technology, especially chipization, thereby realizing the portability of the device.

Description

A rapid detection microfluidic chip

technical field

The invention relates to the technical field of detection equipment, in particular to a microfluidic chip for rapid detection.

Background technique

Microfluidics, or Lab-on-a-chip, refers to chemical or biological labs built on a chip. Physically speaking, a microfluidic chip is a system that manipulates the flow of tiny volumes of fluid in tiny channels or components, where the dimensions of the channels and components are tens to hundreds of micrometers. It integrates basic operation units such as sample preparation, reaction, separation, detection, cell culture, sorting, and lysis involved in the fields of chemistry and biology into a chip of several square centimeters. Microfluidic units such as pumps and microvalves can control the fluid to run through the entire microchannel system, so that the controllable fluid runs through the entire system to achieve various functions in conventional chemical or biological laboratories. Lab-on-a-chip devices automate, miniaturize, and parallelize almost all chemical and biological laboratory functions.

There are usually three methods for virus detection: whole gene sequencing, nucleic acid detection, and immune protein detection. In response to this epidemic, CT technology has also become a very important detection technology, but it is expensive and inconvenient and not suitable for outdoor applications, such as customs and general clinics. In contrast, nucleic acid detection has significant advantages, including automated detection processes, high-throughput quantification, large-scale platform-based detection methods with sample input and data output, and real-time on-site detection. However, most of the nucleic acid detection PCR polymerase chain reaction on the market is not portable, slow and expensive.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a kind of fast detection microfluidic chip, solve the following technical problems:

The object of the present invention can be realized through the following technical solutions:

A rapid detection microfluidic chip, comprising a chip body;

The chip body includes a lower-layer chip, a middle-layer chip and an upper-layer chip in sequence from bottom to top;

The chip body includes a liquid filling port, the liquid filling port is arranged on the surface of the upper chip, and the liquid filling port is arranged in a plurality of groups, and each of the liquid filling ports is respectively connected with the microfluidic structure;

The microfluidic structure is arranged on the side of the middle-layer chip facing the upper-layer chip, and liquid discharge ports are arranged on both sides of the middle-layer chip, and the liquid discharge ports are arranged in multiple groups.

Preferably, a side of the middle-layer chip facing the upper-layer chip is provided with a microfluidic accommodating groove, and the microfluidic accommodating groove is used to carry the microfluidic mechanism.

Preferably, the middle-layer chip is further provided with a liquid input channel and a liquid output channel, the microfluidic structure is connected to the liquid filling port through the liquid input channel, and the end of the microfluidic structure is connected to the liquid discharge port through the liquid output channel.

Preferably, the microfluidic structure includes a microfluidic pipe assembly arranged in the middle, the microfluidic pipe assembly includes a plurality of groups of first microfluidic pipes arranged in parallel and spaced apart, and the ends of each group of the first microfluidic pipes are bent by bending The tubes are connected, and the microfluidic pipeline assembly is composed of a first microfluidic tube and a bent tube to form a serpentine fluid structure.

Preferably, second microfluidic tubes are arranged at the first end of the microfluidic pipeline assembly, respectively, the first microfluidic tube and the second microfluidic tube are communicated through multiple sets of overflow valves, and the second microfluidic tube at the head end connected with the liquid input channel, and the second microfluidic tube at the tail end is connected with the liquid output channel.

Preferably, a plurality of sets of heat insulation mechanisms are further arranged in the microfluidic holding tank, and the heat insulation mechanisms include first heat insulation grooves arranged on opposite sides of the microfluidic structure, and the first heat insulation grooves are composed of a set of The long groove and the end groove are formed, and a second heat insulation groove is arranged between the two sets of short grooves arranged oppositely. A third heat insulating groove is arranged between the two sets of oppositely arranged long grooves, and the liquid output channel penetrates through the long groove gap installed between the third heat insulating groove and the first heat insulating groove.

Preferably, a waste liquid storage cavity is provided on the surface of the lower chip, and the waste liquid storage cavity is used to temporarily store the discharged waste liquid.

Preferably, the surface of the lower chip is further provided with a drain groove and an exhaust hole.

Beneficial effects of the present invention:

(1) The microfluidic chip of the present invention is provided with a heat-insulating tank structure, which can realize rapid temperature rise and fall, thereby greatly shortening the PCR reaction time, and shortening the PCR amplification process that usually takes more than minutes to within minutes. At the same time, the microfluidic chip of the present invention can realize high-precision integration through silicon-based microfluidic chip technology, especially chip formation, thereby realizing the portability and miniaturization of the equipment, and can be mass-produced, which is helpful for reducing equipment costs. cost;

(2) The microfluidic chip of the present invention can be applied to the detection of nucleic acid substances in viruses, bacteria, cells, and body fluids, and can realize rapid detection, which is helpful for rapid screening of populations under epidemic conditions. Therefore, the present invention effectively overcomes various shortcomings in the prior art and has high industrial utilization value.

Description of drawings

The present invention will be further described below in conjunction with the accompanying drawings.

1 is a schematic structural diagram of a rapid detection microfluidic chip of the present invention;

2 is a schematic structural diagram of a thermal insulation groove in a rapid detection microfluidic chip of the present invention;

3 is a schematic structural diagram of a microfluidic structure in a rapid detection microfluidic chip of the present invention;

4 is a schematic structural diagram of the middle and lower layer chips of a rapid detection microfluidic chip of the present invention;

5 is a schematic structural diagram of a microfluidic pipeline assembly in a rapid detection microfluidic chip of the present invention;

In the figure: 1. Upper chip; 2. Middle chip; 3. Lower chip; 4. Liquid filling port; 5. Exhaust hole; 6. Waste liquid storage chamber; 7. Microfluidic holding tank; Slot; 9. Bending tube; 10. Liquid output channel; 11. Third insulation slot; 12. Drainage slot; 13. Drainage port; 14. Second insulation slot; 15. Microfluidic pipeline assembly; 16 , overflow valve; 17, liquid input channel; 18, second microfluidic tube.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Example 1

Please refer to FIGS. 1-5 , the present invention is a microfluidic chip for rapid detection, including a chip body. The chip body includes a lower-layer chip 3 , a middle-layer chip 2 and an upper-layer chip 1 in order from bottom to top. The chip body Including a liquid filling port 4, the liquid filling port 4 is arranged on the surface of the upper chip 1, the liquid filling port 4 is arranged in multiple groups, each of the liquid filling port 4 is respectively connected with a microfluidic structure, and the microfluidic structure is arranged On the side of the middle-layer chip 2 facing the upper-layer chip 1 , the liquid discharge ports 13 are arranged on both sides of the middle-layer chip 2 , and the liquid discharge ports 13 are arranged in multiple groups;

Wherein, the side of the middle-layer chip 2 facing the upper-layer chip 1 is provided with a microfluidic accommodating groove 7, and the microfluidic accommodating groove 7 is used to carry the microfluidic mechanism 18;

Specifically, in this embodiment, the liquid is input into the microfluidic structure through the liquid addition port 4 , and the liquid in the microfluidic structure is discharged through the liquid discharge port 13 .

The middle-layer chip 2 is also provided with a liquid input channel 17 and a liquid output channel 10, the microfluidic structure is connected to the liquid filling port 4 through the liquid input channel 17, and the end of the microfluidic structure is connected to the liquid outlet 13 through the liquid output channel 10. Connection, in the process of adding liquid to the microfluidic structure, the liquid material added to the liquid filling port 4 is added to the microfluidic structure through the liquid input channel 17, and the liquid material in the microfluidic structure is discharged through the liquid discharge port 13. .

Example 2

Please refer to FIGS. 1-4. The present invention is a microfluidic chip for rapid detection, including a chip body. The chip body includes a lower-layer chip 3, a middle-layer chip 2, and an upper-layer chip 1 in order from bottom to top. The chip body includes a plus Liquid port 4, the liquid addition port 4 is arranged on the surface of the upper chip 1, the liquid addition port 4 is arranged in multiple groups, and each of the liquid addition ports 4 is respectively connected with the microfluidic structure, and the microfluidic structure is arranged in the The middle-layer chip 2 faces the side of the upper-layer chip 1, and the two sides of the middle-layer chip 2 are arranged with liquid discharge ports 13, and the liquid discharge ports 13 are arranged in multiple groups;

Wherein, the side of the middle-layer chip 2 facing the upper-layer chip 1 is provided with a microfluidic accommodating groove 7, and the microfluidic accommodating groove 7 is used to carry the microfluidic mechanism 18;

Specifically, in this embodiment, the liquid is input into the microfluidic structure through the liquid addition port 4 , and the liquid in the microfluidic structure is discharged through the liquid discharge port 13 .

The middle-layer chip 2 is also provided with a liquid input channel 17 and a liquid output channel 10, the microfluidic structure is connected to the liquid filling port 4 through the liquid input channel 17, and the end of the microfluidic structure is connected to the liquid outlet 13 through the liquid output channel 10. Connection, in the process of adding liquid to the microfluidic structure, the liquid material added to the liquid filling port 4 is added to the microfluidic structure through the liquid input channel 17, and the liquid material in the microfluidic structure is discharged through the liquid discharge port 13. .

Further, the microfluidic structure includes a microfluidic pipe assembly arranged in the middle, and the microfluidic pipe assembly includes a plurality of groups of first microfluidic pipes 15 arranged in parallel and spaced apart, and the ends of each group of the first microfluidic pipes 15 pass through. The bent tube 9 is connected, and the microfluidic pipeline assembly is composed of the first microfluidic tube 15 and the bent tube 9 to form a serpentine fluid structure;

Wherein, second microfluidic tubes 18 are arranged at the first end of the microfluidic pipeline assembly, respectively, the first microfluidic tube 15 and the second microfluidic tube 18 are communicated through multiple sets of overflow valves 16, and the second microfluidic tube 18 at the head end The microfluidic tube 18 is connected to the liquid input channel 17, and the second microfluidic tube 18 at the tail end is connected to the liquid output channel 10. Specifically, a microfluidic tube assembly provided with a serpentine fluid mechanism is used to make the liquid flow in the microfluidic tube. The structure flows for a longer time, and at the same time, multiple sets of overflow valves 16 are arranged between the first microfluidic tube 15 and the second microfluidic tube 18 to further improve the technical effect.

Example 3

1-5, the present invention is a microfluidic chip for rapid detection, including a chip body, the chip body includes a lower layer chip 3, a middle layer chip 2 and an upper layer chip 1 sequentially from bottom to top, and the chip body includes a plus Liquid port 4, the liquid addition port 4 is arranged on the surface of the upper chip 1, the liquid addition port 4 is arranged in multiple groups, and each of the liquid addition ports 4 is respectively connected with the microfluidic structure, and the microfluidic structure is arranged in the The middle-layer chip 2 faces the side of the upper-layer chip 1, and the two sides of the middle-layer chip 2 are arranged with liquid discharge ports 13, and the liquid discharge ports 13 are arranged in multiple groups;

Wherein, the side of the middle-layer chip 2 facing the upper-layer chip 1 is provided with a microfluidic accommodating groove 7, and the microfluidic accommodating groove 7 is used to carry the microfluidic mechanism 18;

Specifically, in this embodiment, the liquid is input into the microfluidic structure through the liquid addition port 4 , and the liquid in the microfluidic structure is discharged through the liquid discharge port 13 .

The middle-layer chip 2 is also provided with a liquid input channel 17 and a liquid output channel 10, the microfluidic structure is connected to the liquid filling port 4 through the liquid input channel 17, and the end of the microfluidic structure is connected to the liquid outlet 13 through the liquid output channel 10. Connection, in the process of adding liquid to the microfluidic structure, the liquid material added to the liquid filling port 4 is added to the microfluidic structure through the liquid input channel 17, and the liquid material in the microfluidic structure is discharged through the liquid discharge port 13. .

Further, the microfluidic structure includes a microfluidic pipe assembly arranged in the middle, and the microfluidic pipe assembly includes a plurality of groups of first microfluidic pipes 15 arranged in parallel and spaced apart, and the ends of each group of the first microfluidic pipes 15 pass through. The bent tube 9 is connected, and the microfluidic pipeline assembly is composed of the first microfluidic tube 15 and the bent tube 9 to form a serpentine fluid structure;

Wherein, second microfluidic tubes 18 are arranged at the first end of the microfluidic pipeline assembly, respectively, the first microfluidic tube 15 and the second microfluidic tube 18 are communicated through multiple sets of overflow valves 16, and the second microfluidic tube 18 at the head end The microfluidic tube 18 is connected to the liquid input channel 17, and the second microfluidic tube 18 at the tail end is connected to the liquid output channel 10. Specifically, a microfluidic tube assembly provided with a serpentine fluid mechanism is used to make the liquid flow in the microfluidic tube. The structure flows for a longer time, and at the same time, multiple sets of overflow valves 16 are arranged between the first microfluidic tube 15 and the second microfluidic tube 18 to further improve the technical effect.

Further, the microfluidic holding tank 7 is also provided with a plurality of sets of heat insulation mechanisms, and the heat insulation mechanisms include first heat insulation grooves 8 arranged on opposite sides of the microfluidic structure. The first heat insulation grooves 8 It is composed of a set of long grooves and end-shaped grooves, and a second heat insulation groove 14 is arranged between the two sets of short grooves arranged oppositely. The liquid input channel 17 is installed through the second heat insulation groove 14 and the first heat insulation groove. 8 of the short groove gap, a third heat insulation groove 11 is arranged between the two sets of long grooves arranged opposite to each other, and the liquid output channel 10 runs through the long grooves installed in the third heat insulation groove 11 and the first heat insulation groove 8 gap.

The surface of the lower chip 3 is provided with a waste liquid storage chamber 6 for temporarily storing the discharged waste liquid. The surface of the lower chip 3 is also provided with a drainage groove 12 and an exhaust hole 5 .

The working principle of the present invention: The microfluidic chip of the present invention is provided with a heat-insulating groove structure, which can realize rapid temperature rise and fall, thereby greatly shortening the PCR reaction time, and shortening the PCR amplification process that usually takes more than 30 minutes to less than 5 minutes. At the same time, the microfluidic chip of the present invention can realize high-precision integration through silicon-based microfluidic chip technology, especially chip formation, thereby realizing the portability and miniaturization of the equipment, and can be mass-produced, which is helpful for reducing equipment costs. cost. The microfluidic chip of the present invention can be applied to the detection of nucleic acid substances in viruses, bacteria, cells, and body fluids, and can realize rapid detection, which is helpful for rapid screening of people in an epidemic state. Therefore, the present invention effectively overcomes various shortcomings in the prior art and has high industrial utilization value.

In the description of the present invention, it should be understood that the terms "upper", "lower", "left", "right", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for convenience The invention is described and simplified without indicating or implying that the device or element referred to must have a particular orientation, as well as a particular orientation configuration and operation, and therefore should not be construed as limiting the invention. In addition, "first" and "second" are for descriptive purposes only, and cannot be understood as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature delimited with "first", "second" may expressly or implicitly include one or more of that feature. In the description of the present invention, unless otherwise specified, "plurality" means two or more.

In the description of the present invention, it should be noted that, unless otherwise expressly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection or a detachable connection , or integrally connected; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or the internal communication between the two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

An embodiment of the present invention has been described in detail above, but the content is only a preferred embodiment of the present invention, and cannot be considered to limit the scope of the present invention. All equivalent changes and improvements made according to the scope of the application of the present invention should still belong to the scope of the patent of the present invention.

Claims (8)

1. a fast detection microfluidic chip, is characterized in that, comprises chip body; The chip body includes a lower-layer chip (3), a middle-layer chip (2) and an upper-layer chip (1) in sequence from bottom to top; The chip body includes a liquid addition port (4), the liquid addition port (4) is arranged on the surface of the upper chip (1), and the liquid addition ports (4) are arranged in multiple groups, and each liquid addition port (4) ) are respectively connected with the microfluidic structure; The microfluidic structure is arranged on the side of the middle-layer chip (2) facing the upper-layer chip (1), and liquid discharge ports (13) are arranged on both sides of the middle-layer chip (2), and the liquid discharge ports (13) Arrange multiple groups. 2. A microfluidic chip for rapid detection according to claim 1, characterized in that, the side of the middle-layer chip (2) facing the upper-layer chip (1) is provided with a microfluidic accommodating groove (7), and the microfluidic accommodating groove (7) is The groove (7) is used to carry the microfluidic mechanism (18). 3. A kind of fast detection microfluidic chip according to claim 2, characterized in that, the middle-layer chip (2) is also provided with a liquid input channel (17) and a liquid output channel (10), and the microfluidic structure passes through The liquid input channel (17) is connected with the liquid addition port (4), and the end of the microfluidic structure is connected with the liquid discharge port (13) through the liquid output channel (10). 4. A microfluidic chip for rapid detection according to claim 3, wherein the microfluidic structure comprises a microfluidic conduit assembly arranged in the middle, and the microfluidic conduit assembly comprises a plurality of groups of A microfluidic tube (15), the ends of the first microfluidic tubes (15) in each group are connected through a bent tube (9), and the microfluidic tube assembly is composed of a first microfluidic tube (15) and a bent tube ( 9) Constitute a serpentine fluid structure. 5. A microfluidic chip for rapid detection according to claim 4, characterized in that a second microfluidic tube (18) is arranged at the first end of the microfluidic pipeline assembly, and the first microfluidic tube (15) is arranged respectively. ) is communicated with the second microfluidic tube (18) through multiple sets of overflow valves (16), the second microfluidic tube (18) at the head end is connected with the liquid input channel (17), and the second microfluidic tube (18) at the tail end is connected with the liquid input channel (17) The pipe (18) is connected to the liquid output channel (10). 6. A microfluidic chip for rapid detection according to claim 5, characterized in that a plurality of groups of heat insulation mechanisms are further arranged in the microfluidic holding tank (7), and the heat insulation mechanisms comprise The first heat insulation grooves (8) on both sides of the fluid structure, the first heat insulation grooves (8) are composed of a set of long grooves and end grooves, and a second heat insulation groove is arranged between the two sets of short grooves arranged oppositely. The groove (14), the liquid input channel (17) is installed in the short groove gap between the second heat insulation groove (14) and the first heat insulation groove (8), and a second heat insulation groove is arranged between the two sets of long grooves arranged opposite to each other. Three heat-insulating grooves (11), the liquid output channel (10) penetrates through the elongated groove gap installed between the third heat-insulating groove (11) and the first heat-insulating groove (8). 7. A rapid detection microfluidic chip according to claim 1, characterized in that, a waste liquid storage cavity (6) is provided on the surface of the lower chip (3), and the waste liquid storage cavity (6) is used for Temporary storage of the discharged waste liquid. 8 . The microfluidic chip for rapid detection according to claim 7 , wherein the surface of the lower chip ( 3 ) is further provided with a drain groove ( 12 ) and an exhaust hole ( 5 ). 9 .

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