CN114921327B - Microorganism spore's culture apparatus - Google Patents
Microorganism spore's culture apparatus Download PDFInfo
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- CN114921327B CN114921327B CN202210298132.1A CN202210298132A CN114921327B CN 114921327 B CN114921327 B CN 114921327B CN 202210298132 A CN202210298132 A CN 202210298132A CN 114921327 B CN114921327 B CN 114921327B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a culture device for microbial spores, and belongs to the technical field of microbial culture. A microbial spore cultivation apparatus comprising: the invention can improve the culture efficiency, save the culture time and reduce the culture error of the same batch.
Description
Technical Field
The invention belongs to the technical field of microorganism culture, and particularly relates to culture equipment for microorganism spores.
Background
The microbial fertilizer is a living body product containing special functional microbial strains, the quality guarantee period of the microbial fertilizer plays a vital role in the commercial development and application of the microbial fertilizer, and as is well known, microbial spores and spores are more durable to store than thalli and mycelia, so that living organisms in the microbial fertilizer should exist in the forms of spores and spores as much as possible, and fermentation conditions are inconsistent in the existing microbial spore culture process, so that the reproducibility is poor.
Disclosure of Invention
The invention aims to solve the problem of poor reproducibility caused by inconsistent fermentation conditions in the conventional microbial spore culture process, and provides microbial spore culture equipment.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a microbial spore cultivation apparatus comprising: support, set up shell mechanism, lifting mechanism, rotatory reinforced mechanism and control panel on the support, lifting mechanism and the coaxial swing joint of shell mechanism, control panel fixed connection in the support deviate from one side of shell mechanism, rotatory reinforced mechanism fixed connection in the support deviate from one side of control panel, lifting mechanism and support top fixed connection, shell mechanism and support fixed connection, control panel be used for controlling the operation of shell mechanism, lifting mechanism and rotatory reinforced mechanism.
Preferably, the shell mechanism include vertical first drum that arranges and be connected with the support, set up in the blast pipe of the outside bottom of first drum circumference and coaxial sealed lid that sets up in first drum bottom, first drum inside cavity and both ends set up to the opening, the blast pipe radially extends along first drum and blast pipe and first drum inner chamber intercommunication, the annular has been seted up to first drum bottom coaxial, be provided with a plurality of first guide blocks along the circumferencial direction in the annular, sealed lid top coaxial be provided with the annular plate that matches with the annular, the annular plate interior round surface seted up with first guide block first guide way that matches, the annular plate circumference outer wall has seted up the second screw hole that runs through the annular plate lateral wall, second screw hole matching is provided with first bolt, sealed lid center department coaxial offer the through hole, the through hole internal match is provided with the rubber slab, the rubber slab bottom coaxial fan that is provided with, sealed lid bottom is provided with first screw hole, the steam pipe with first drum inner chamber intercommunication, first screw hole internal match is provided with the second bolt, be provided with the valve on the steam pipe.
Preferably, the rotary feeding mechanism comprises a second motor, a speed reducer, a connecting shaft and a second cylinder, wherein the second motor is connected with a support and is vertically arranged, the speed reducer is coaxially arranged at the bottom end of the second motor, the connecting shaft is coaxially arranged at the bottom end of the speed reducer, the second cylinder is matched with the first cylinder, the second cylinder is in threaded connection with the first cylinder, a gear is coaxially arranged at the bottom end of the connecting shaft, an outer ring gear meshed with the gear is coaxially sleeved outside the top end of the second cylinder, a plurality of second guide grooves are uniformly arranged outside the second cylinder at intervals along the circumferential direction, the second guide grooves extend along the length direction of the second cylinder, a second guide block matched with the second guide grooves is arranged on the inner circular surface of the outer ring gear, a bearing is coaxially arranged at the bottom end of the outer ring gear, the outer ring gear is connected with the support, a dropper extending radially along the second cylinder is arranged at the bottom end of the inner circular surface of the second cylinder, the dropper is connected with a liquid adding pipe extending to the top end of the second cylinder, and a monitoring probe is arranged at the bottom of the suspension end.
Preferably, the lifting mechanism comprises a screw plate matched with the inner cavity of the second cylinder, a plurality of culture members uniformly arranged at intervals along the extending direction of the screw plate, a pressure sensor and a temperature sensor arranged on the culture members, wherein the screw plate is in threaded connection with the second cylinder, threads between the second cylinder and the first cylinder are matched with the screw plate, the culture members comprise a supporting plate arranged on the screw plate, a fixing groove formed in the top of the supporting plate and a culture dish matched with the fixing groove, the suspension end of the dropper is positioned right above the culture dish, a third threaded hole matched with the second bolt is formed in the bottom end of the screw plate, a rubber piston matched with the inner cavity of the second cylinder is arranged at the top end of the screw plate, a sterile air pipe communicated with the inner cavity of the second cylinder is arranged at one side of the top end of the rubber piston, a telescopic rod is coaxially arranged at the top end of the rubber piston, and a first motor connected with a bracket is coaxially arranged at the top of the telescopic rod.
Preferably, the fan, the first motor, the pressure sensor, the temperature sensor, the second motor and the monitoring probe are all electrically connected with the control panel and controlled to operate by the control panel.
Preferably, the diameter of the outer gear ring is twice the diameter of the gear.
Compared with the prior art, the invention provides the culture equipment of the microbial spores, which has the following beneficial effects:
1. When the screw plate fixing device is used, the sealing cover is firstly opened, then the first motor is controlled to rotate through the control panel, the screw plate is driven to rotate through the telescopic rod and the rubber piston by the first motor, the screw plate rotates to move downwards and moves out of the inner cavity of the second cylinder, when the screw plate is completely moved out, the first motor is closed, then a culture dish is added on the fixing groove, then the screw plate is controlled to reset, the sealing cover is used for resealing, and the second bolt is rotated to enable the second bolt to be screwed into the third threaded hole to fix the screw plate.
2. According to the invention, steam is introduced through the steam pipe, the rubber plate is turned over to enable the fan to face upwards, the fan is started to blow the steam upwards, the rapid diffusion of the steam is ensured, the temperature and the air pressure are monitored through the pressure sensor and the temperature sensor, the second cylinder inner cavity is kept at 121 ℃ and 0.12MPa for 30 minutes of continuous sterilization, the valve is closed, then sterile air is introduced through the sterile air pipe for cooling, the redundant air is discharged through the exhaust pipe, then the sterile air pipe and the exhaust pipe are sealed, and by arranging the spiral piece, the small space of the spare space can be ensured, the utilization efficiency is improved, the efficient sterilization and cooling are ensured, and the working time is reduced.
3. The invention controls the second motor to start, the second motor drives the outer gear ring to rotate through the speed reducer, the connecting shaft and the gear, the outer gear ring drives the second cylinder to spirally rise, the second cylinder drives the dropper and the monitoring probe to spirally rise, the dropper passes through each culture dish and conveys nutrient solution and spore suspension to the culture dish, and the monitoring probe observes and controls the conveying amount of the nutrient solution, so that the same culture condition of the same batch is ensured, and the error is reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of a housing mechanism, a pulling mechanism and a rotary charging mechanism according to the present invention;
FIG. 3 is a schematic diagram of a second embodiment of the housing mechanism, the pulling mechanism and the rotary charging mechanism of the present invention;
FIG. 4 is a schematic diagram of a housing mechanism, a pulling mechanism and a rotary charging mechanism according to the present invention;
FIG. 5 is a schematic diagram of a pulling mechanism and a rotary feeding mechanism according to the present invention;
FIG. 6 is a second schematic diagram of the structure of the pulling mechanism and the rotary feeding mechanism of the present invention;
FIG. 7 is a schematic view of a pulling mechanism according to the present invention;
FIG. 8 is a schematic view of a rotary charging mechanism according to the present invention;
FIG. 9 is a schematic view of the housing mechanism, the pulling mechanism and the rotary charging mechanism of the present invention;
FIG. 10 is a schematic view of a housing mechanism and a pulling mechanism according to the present invention;
Fig. 11 is a schematic structural view of a housing mechanism according to the present invention.
The reference numerals in the figures illustrate:
10. A bracket; 20. a housing mechanism; 210. a first cylinder; 211. a ring groove; 212. a first guide block; 220. an exhaust pipe; 230. sealing cover; 231. a ring plate; 232. a first guide groove; 233. a through hole; 234. a first threaded hole; 235. a second threaded hole; 236. a first bolt; 240. a rubber plate; 250. a fan; 260. a second bolt; 270. a steam pipe; 271. a valve; 280. a third threaded hole; 30. a lifting mechanism; 310. a first motor; 320. a telescopic rod; 330. a rubber piston; 340. a sterile air tube; 350. a spiral sheet; 360. a culture member; 361. a support plate; 362. a fixing groove; 363. a culture dish; 370. a pressure sensor; 380. a temperature sensor; 40. a rotary feeding mechanism; 410. a second motor; 420. a speed reducer; 430. a connecting shaft; 440. a gear; 450. an outer ring gear; 451. a second guide block; 460. a bearing; 470. a second cylinder; 471. a second guide groove; 472. a dropper; 473. monitoring a probe; 50. and a control panel.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the mechanisms or elements referred to must have a particular orientation, be configured and operated in a particular orientation, and thus should not be construed as limiting the present invention.
As shown in fig. 1 to 11, a microorganism spore culturing apparatus comprising: support 10, set up casing mechanism 20, pulling mechanism 30, rotatory feeding mechanism 40 and control panel 50 on support 10, pulling mechanism 30 and the coaxial swing joint of casing mechanism 20, control panel 50 fixed connection in support 10 deviate from one side of casing mechanism 20, rotatory feeding mechanism 40 fixed connection in support 10 deviate from one side of control panel 50, pulling mechanism 30 and support 10 top fixed connection, casing mechanism 20 and support 10 fixed connection, control panel 50 be used for controlling the operation of casing mechanism 20, pulling mechanism 30 and rotatory feeding mechanism 40.
The shell mechanism 20 comprises a first cylinder 210 vertically arranged and connected with the support 10, an exhaust pipe 220 arranged at the bottom end outside the circumference of the first cylinder 210 and a sealing cover 230 coaxially arranged at the bottom end of the first cylinder 210, wherein the first cylinder 210 is hollow, two ends of the first cylinder are provided with openings, the exhaust pipe 220 extends radially along the first cylinder 210, the exhaust pipe 220 is communicated with the inner cavity of the first cylinder 210, an annular groove 211 is coaxially arranged at the bottom end of the first cylinder 210, a plurality of first guide blocks 212 are arranged in the annular groove 211 along the circumferential direction, a ring plate 231 matched with the annular groove 211 is coaxially arranged at the top of the sealing cover 230, a first guide groove 232 matched with the first guide blocks 212 is formed in the inner circle surface of the ring plate 231, a second threaded hole 235 penetrating through the side wall of the ring plate 231 is formed in the circumference outer wall of the ring plate 231, a first bolt 236 is arranged in the second threaded hole 235 in a matched manner, a through hole 233 is coaxially formed in the center of the sealing cover 230, a rubber plate 240 is arranged in the penetrating hole 233 in a matched manner, a fan 250 is coaxially arranged at the bottom of the rubber plate 240, a first threaded hole 234 is arranged at the bottom of the sealing cover, a steam pipe 270 is communicated with the inner cavity of the first sealing cover 210, a valve 270 is arranged in the first threaded hole 270, and a valve is arranged in the second valve 270 is matched with the second bolt 236.
The rotary feeding mechanism 40 comprises a second motor 410, a speed reducer 420, a connecting shaft 430 and a second cylinder 470, wherein the second motor 410 is connected with the support 10 and is vertically arranged, the speed reducer 420 is coaxially arranged at the bottom end of the second motor 410, the connecting shaft 430 is coaxially arranged at the bottom end of the speed reducer 420, the second cylinder 470 is matched with the inner cavity of the first cylinder 210, the second cylinder 470 is in threaded connection with the first cylinder 210, a gear 440 is coaxially arranged at the bottom end of the connecting shaft 430, an outer gear ring 450 meshed with the gear 440 is coaxially sleeved outside the top end of the second cylinder 470, a plurality of second guide grooves 471 are uniformly arranged outside the second cylinder 470 at intervals along the circumferential direction, the second guide grooves 471 extend along the length direction of the second cylinder 470, a second guide block 451 matched with the second guide grooves 471 is arranged on the inner circle surface of the outer gear ring 450, a bearing 460 is coaxially arranged at the bottom end of the outer circle of the outer gear ring 450, the bearing 460 is connected with the support 10, a dropper 472 radially extends along the second cylinder 470, the dropper 472 extends to the top end of the second cylinder 470 through connection, and a monitoring probe 473 is arranged at the bottom of the suspension end of the dropper.
The lifting mechanism 30 comprises a spiral sheet 350 matched with the inner cavity of the second cylinder 470, a plurality of culture members 360 uniformly arranged at intervals along the extending direction of the spiral sheet 350, a pressure sensor 370 and a temperature sensor 380 arranged on the culture members 360, the spiral sheet 350 is in threaded connection with the second cylinder 470, threads between the second cylinder 470 and the first cylinder 210 are matched with the spiral sheet 350, the culture members 360 comprise a supporting plate 361 arranged on the spiral sheet 350, a fixing groove 362 arranged at the top of the supporting plate 361, a culture dish 363 matched with the fixing groove 362, the suspension end of the dropper 472 is positioned right above the culture dish 363, a third threaded hole 280 matched with the second bolt 260 is arranged at the bottom end of the spiral sheet 350, a rubber piston 330 matched with the inner cavity of the second cylinder 470 is arranged at the top end of the spiral sheet 350, a sterile air pipe 340 communicated with the inner cavity of the second cylinder 470 is arranged at one side of the top of the rubber piston 330, a telescopic rod 320 is coaxially arranged at the top of the rubber piston 330, and a first motor 310 connected with the bracket 10 is coaxially arranged at the top of the telescopic rod 320.
The fan 250, the first motor 310, the pressure sensor 370, the temperature sensor 380, the second motor 410, and the monitor probe 473 are all electrically connected to the control panel 50 and controlled to operate by the control panel 50.
More fully, the diameter of the outer gear 450 is twice the diameter of the gear 440; the moment is increased to facilitate pushing the second cylinder 470 to rotate.
When the screw plate 350 is used, the sealing cover 230 is firstly opened, then the first motor 310 is controlled to rotate through the control panel 50, the first motor 310 drives the screw plate 350 to rotate through the telescopic rod 320 and the rubber piston 330, the screw plate 350 rotates to move downwards and moves out of the inner cavity of the second cylinder 470, when the screw plate 350 is completely moved out, the first motor 310 is closed, then the culture dish 363 is added on the fixing groove 362, then the screw plate 350 is controlled to reset, the sealing cover 230 is used for resealing, and the second bolt 260 is rotated to enable the second bolt 260 to be screwed into the third threaded hole 280 to fix the screw plate 350.
The steam is introduced through the steam pipe 270, the rubber plate 240 is turned over to enable the fan 250 to face upwards, the fan 250 is started, the fan 250 blows the steam upwards, the rapid diffusion of the steam is guaranteed, the temperature and the air pressure are monitored through the pressure sensor 370 and the temperature sensor 380, the inner cavity 121 ℃ of the second cylinder 470 is kept for 30 minutes after continuous sterilization at 0.12MPa, the valve 271 is closed, then sterile air is introduced through the sterile air pipe 340 for cooling, redundant air is discharged through the air exhaust pipe 220, the sterile air pipe 340 and the air exhaust pipe 220 are sealed later, the empty space is small through the spiral piece 350, the utilization efficiency is improved, the efficient sterilization and cooling are guaranteed, and the working time is shortened.
The second motor 410 is controlled to start, the second motor 410 drives the outer gear ring 450 to rotate through the speed reducer 420, the connecting shaft 430 and the gear 440, the outer gear ring 450 drives the second cylinder 470 to spirally rise, the second cylinder 470 drives the dropper 472 and the monitoring probe 473 to spirally rise, the dropper 472 passes through each culture dish 363 and conveys nutrient solution and spore suspension to the culture dishes 363, and the conveying amount of the nutrient solution is observed and controlled through the monitoring probe 473, so that the same culture condition of the same batch is ensured, and the error is reduced.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (5)
1. A microbial spore culture apparatus, comprising: the device comprises a support (10), a shell mechanism (20) arranged on the support (10), a lifting mechanism (30), a rotary feeding mechanism (40) and a control panel (50), wherein the lifting mechanism (30) is coaxially and movably connected with the shell mechanism (20), the control panel (50) is fixedly connected to one side, deviating from the shell mechanism (20), of the support (10), the rotary feeding mechanism (40) is fixedly connected to one side, deviating from the control panel (50), of the support (10), the lifting mechanism (30) is fixedly connected with the top of the support (10), the shell mechanism (20) is fixedly connected with the support (10), and the control panel (50) is used for controlling the operation of the shell mechanism (20), the lifting mechanism (30) and the rotary feeding mechanism (40);
The shell mechanism (20) comprises a first cylinder (210) which is vertically arranged and connected with the bracket (10), wherein the first cylinder (210) is hollow and is provided with openings at two ends;
The rotary feeding mechanism (40) comprises a second motor (410) connected with the bracket (10) and vertically arranged, a speed reducer (420) coaxially arranged at the bottom end of the second motor (410), a connecting shaft (430) coaxially arranged at the bottom end of the speed reducer (420) and a second cylinder (470) matched with the inner cavity of the first cylinder (210), the second cylinder (470) is in threaded connection with the first cylinder (210), a gear (440) is coaxially arranged at the bottom end of the connecting shaft (430), an outer ring (450) meshed with the gear (440) is coaxially sleeved outside the top end of the second cylinder (470), a plurality of second guide grooves (471) are uniformly arranged outside the second cylinder (470) at intervals along the circumferential direction, the second guide grooves (471) extend along the length direction of the second cylinder (470), a second guide block (451) matched with the second guide grooves (471) is arranged on the inner circular surface of the outer ring (450), a bearing (460) is arranged on the outer bottom end of the outer sleeve of the second cylinder (450) in a same way as the bearing, the outer ring is connected with the bracket (10), the second cylinder (470) is provided with an outer ring, the second cylinder (470) is provided with a liquid adding pipe (470) extending along the radial direction of the second cylinder (470) and extends to the top end of the second cylinder (470), the bottom of the suspension end of the dropper (472) is provided with a monitoring probe (473);
The lifting mechanism (30) comprises a spiral sheet (350) which is matched with the inner cavity of the second cylinder (470), and a plurality of culture members (360) which are uniformly arranged at intervals along the extending direction of the spiral sheet (350); the screw plate (350) is in threaded connection with the second cylinder (470), and the threads between the second cylinder (470) and the first cylinder (210) are matched with the screw plate (350); the culture component (360) comprises a support plate (361) arranged on the spiral piece (350), a fixing groove (362) formed in the top of the support plate (361), and a culture dish (363) matched with the fixing groove (362), wherein the suspension end of the dropper (472) is positioned right above the culture dish (363).
2. The apparatus for culturing microbial spores of claim 1, wherein: the shell mechanism (20) include exhaust pipe (220) and sealed lid (230) of coaxial setting in first drum (210) bottom in the outside bottom of circumference of first drum (210), exhaust pipe (220) radially extend and exhaust pipe (220) and first drum (210) inner chamber intercommunication along first drum (210), annular groove (211) have been seted up to first drum (210) bottom coaxial, annular groove (211) are provided with a plurality of first guide blocks (212) along the circumferencial direction in, sealed lid (230) top coaxial be provided with annular groove (211) matched ring plate (231), ring plate (231) interior round surface set up with first guide block (212) matched first guide groove (232), ring plate (231) circumference outer wall set up through ring plate (231) second screw hole (235), second screw hole (235) in-match is provided with first bolt (236), sealed lid (230) center department coaxial offer through-hole (233), through-hole (233) in-match is provided with rubber plate (240), rubber plate (240) bottom coaxial sealed lid (250), first screw hole (234) and first drum (234) inner chamber (234) are provided with first screw hole (234), a valve (271) is provided on the steam pipe (270).
3. A microbial spore culture apparatus as claimed in claim 2, wherein: the lifting mechanism (30) comprises a pressure sensor (370) and a temperature sensor (380) which are arranged on a culture member (360), a third threaded hole (280) matched with the second bolt (260) is formed in the bottom end of the spiral piece (350), a rubber piston (330) matched with the inner cavity of the second cylinder (470) is arranged at the top end of the spiral piece (350), an aseptic air pipe (340) communicated with the inner cavity of the second cylinder (470) is arranged at one side of the top end of the rubber piston (330), a telescopic rod (320) is coaxially arranged at the top end of the rubber piston (330), and a first motor (310) connected with the bracket (10) is coaxially arranged at the top end of the telescopic rod (320).
4. A microbial spore culture apparatus as claimed in claim 3, wherein: the fan (250), the first motor (310), the pressure sensor (370), the temperature sensor (380), the second motor (410) and the monitoring probe (473) are all electrically connected with the control panel (50) and controlled to operate by the control panel (50).
5. The apparatus for culturing microbial spores of claim 1, wherein: the diameter of the outer gear ring (450) is twice the diameter of the gear (440).
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