CN114947696A - White light-narrow band-fluorescence integrated endoscope and use method thereof - Google Patents

Abstract

The invention provides a white light-narrow-band-fluorescence integrated endoscope and a method of using the same, comprising: a light source part, a light source control unit and an endoscope part; White light and narrow-band light of various wavelengths, the narrow-band light includes fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be inspected to generate fluorescence; the light source control unit controls the type of illumination light provided by the light source part; the endoscope part includes endoscopic detection The endoscope detector is used to receive the reflected light after the illumination light is reflected by the biological tissue to be inspected and the fluorescence generated by the excitation of the photosensitizer on the biological tissue by the fluorescence excitation light. The plate enters the endoscope detector; the notch wavelength of the notch plate is equal to the wavelength of the fluorescence excitation light. Combining the multi-spectral narrowband function and the fluorescence function can significantly reduce the complexity of the endoscope structure on the premise of ensuring the imaging quality and acquisition speed.

Description

A white light-narrow-band-fluorescence integrated endoscope and method of using the same

technical field

The invention belongs to the field of endoscopes, and particularly relates to a white light-narrow-band-fluorescence integrated endoscope and a method for using the same.

Background technique

White light endoscopy, narrow-band endoscopy, polarized endoscopy and fluorescence endoscopy can respectively provide structural and functional signals of tumors. In particular, narrow-band signals are usually sensitive to functional information such as blood oxygen and submucosal microvessels, while polarized signals are chiral molecules. Sensitive to birefringence signals, and on the basis of fluorescence signals, functional information such as the concentration of photosensitizer probes can be used to further prompt tumors. Various functional signals complement each other. Image recognition and signal filtering techniques are used to perform multi-source data analysis. Fusion analysis improves the algorithm's ability to identify tumors.

However, at present, the probes or imaging modules of chromoendoscopy, confocal microscopic magnifying endoscopy, and ultrasound endoscopy are not compatible with white light endoscopy, which actually increases the difficulty of multimodal detection. For example, it is often difficult to qualitatively diagnose gastrointestinal tumors only by white light endoscopy, especially in many cases, it is necessary to further qualitatively diagnose tumors by means of magnifying endoscopy, chromoendoscopy and pathological biopsy, and sometimes it is necessary to make auxiliary judgments on submucosal tissue . For another example, in the process of photodynamic therapy, the detection of oxygen content and photosensitizer concentration are two important parts of photodynamic dose monitoring. Among them, the detection of oxygen content requires the detection of hemoglobin using a multispectral narrow-band endoscope, and the detection of the photosensitizer concentration requires a fluorescence channel. Under the current technology, it is difficult to perform integrated detection of the target tissue under the same endoscope, and it needs to be switched by a mechanical device. The working nature of the endoscope determines that the volume of the endoscope module is inevitably increased through mechanical devices, which in fact limits its application range. For example, the white light-microscope integrated endoscope needs to design a complex optical lens-zoom optical path, and its volume is at least doubled. The narrow-band-fluorescence endoscope needs to design the light splitting optical path, which greatly increases the volume on the one hand, and reduces the utilization rate of illumination in other bands on the other hand. Therefore, there is currently no integrated endoscope for commercial applications on the market. For example, Olympus' NBI endoscopes typically detect narrow-band reflected light at 415 and 540 nm, and the developed Photoelectric Composite Stain Imaging Technology (VIST) uses a single wavelength combined with the RGB channels of the imaging CCD for composite staining. These methods focus on contrasting absorption caused by microvessels and cannot detect spectral information such as lipids or autofluorescence. Imperial College London has developed a hyperspectral narrow-band endoscope using supercontinuum light sources, but supercontinuum light sources are very expensive.

Concentration monitoring of photosensitizers is the most important part of photodynamic dose monitoring. By detecting the excitation fluorescence intensity of the photosensitizer, various information can be obtained, such as the concentration, targeting and spatial distribution of the photosensitizer. Fluorescence endoscopy is mainly used to detect the concentration of photosensitizers. Patent No. CN 110772208 A discloses a fluorescent endoscope device, which collects multiple fluorescent image sub-blocks of a subject through a compound eye imaging device, and then performs pixel coordinate registration on these fluorescent sub-blocks, and finally Based on the pixel registration coordinates of each pixel point in each fluorescent image sub-block, pixel restoration is performed to superimpose, thereby generating a fluorescent image of the subject. Although this method can obtain high-quality fluorescence images, it requires multiple detection channels, and its volume is difficult to reduce. Therefore, its practical application is extremely limited, and the algorithm is extremely complex, which requires high performance of image processing equipment.

Patents CN109758094A and CN108577791A disclose a fluorescence navigation endoscope system and a method for enhancing fluorescence imaging sensitivity. The method separates fluorescence and white light through a light splitting optical path, which is difficult to combine with narrow-band reflective endoscopes, because each additional optical channel, On the one hand, more cameras and beam splitting optical paths are required, which greatly increases the cost. On the other hand, it is difficult for the endoscopic probe to integrate more optical devices.

Patent document CN109758094A discloses a micro-endoscopy system, which draws out the image through an imaging fiber, so as to realize optical path splitting in vitro, so that although it is not limited by the volume of the body, the resolution of the imaging fiber is limited. For example, Sumita Optics is known as the industry-leading imaging Optical fiber can only achieve a resolution of 100,000 pixels, which is far from the current clinical requirements of 1080P or even 4K resolution. Patent document CN108670203A discloses a narrow-band-fluorescence imaging optical path, which uses three sensors and corresponding beam splitting optical paths, which greatly increases its volume and design complexity, and is difficult to apply to electronic endoscopes. Patent document CN110731748A discloses an electronic endoscope with one narrow-band light and one fluorescent coupling, using two splitting optical paths and one imaging chip, the upper limit of which can handle a single wavelength reflected light and single wavelength fluorescence.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a white light-narrowband-fluorescence integrated endoscope and a method of using the same, which combine the white light function, the multispectral narrowband function and the fluorescence function, and under the premise of ensuring the imaging quality and acquisition speed, can Significantly reduces the complexity of the endoscope structure,

The present invention is achieved through the following technical solutions:

A white light-narrowband-fluorescence integrated endoscope, comprising: a light source part, a light source control unit and an endoscope part;

The light source part is used to provide illumination light illuminating the biological tissue to be inspected, the types of the illumination light include white light and narrow-band light of various wavelengths, and the narrow-band light includes fluorescence excitation light; the fluorescence excitation light can excite the inspected The photosensitizer on biological tissue produces fluorescence;

The light source control unit is used to control the type of illumination light provided by the light source part;

The endoscope part includes an endoscope detector and a notch plate; the endoscope detector is used to receive the reflected light from the illumination light provided by the light source part and the reflected light from the inspected biological tissue and the fluorescence provided by the light source part of the photosensitizer on the inspected biological tissue. The fluorescence generated by the excitation light is excited to obtain an optical signal. Both the reflected light and the fluorescence enter the endoscope detector through the notch plate; the notch wavelength of the notch plate is equal to the wavelength of the fluorescence excitation light.

Preferably, the endoscope part further comprises a holder, an endoscope detector channel is opened inside the holder, an endoscope detector is installed in the endoscope detector channel, one end face of the holder is provided with a groove, and the groove It is coaxial with and communicated with the channel of the endoscope detector, and a notch plate is installed in the groove.

Further, the holder is provided with a light guide channel, the axis of the light guide channel is parallel to the axis of the endoscope detector channel; the illumination light provided by the light source part is irradiated on the biological tissue to be inspected through the light guide channel.

Further, two light guide channels are provided, and the two light guide channels are symmetrical with respect to the endoscope detector channel.

Preferably, it also includes a data acquisition part, the data acquisition part includes an acquisition card and a memory card, the acquisition card is used to receive the optical signal of the endoscope detector and the imaging mode signal of the light source control unit, and process the tissue image; The tissue images obtained by the capture card are stored.

Further, a display is also included, and the display is used to display the tissue images obtained by the acquisition part in real time.

Preferably, the light source part includes a xenon lamp light source, a focusing lens group and a filter wheel; the focusing lens group includes a first focusing lens and a second focusing lens; the first focusing lens and the filter are sequentially arranged in the direction of the emitted light of the xenon lamp source. wheel and second focusing lens.

Preferably, the light source part includes a xenon lamp light source, a focusing lens group and a tunable liquid crystal filter LCTF; the focusing lens group includes a first focusing lens and a second focusing lens; the first focusing lens, the adjustable Tune the liquid crystal filter LCTF and the second focusing lens.

The method for using the white light-narrowband-fluorescence integrated endoscope includes a white light imaging mode, a narrowband imaging mode and a fluorescence imaging mode;

If the white light imaging mode is selected, the light source control unit controls the light source part to emit white light, which is irradiated on the inspected biological tissue through the light guide channel, and the reflected light of the white light reflected by the inspected biological tissue is detected by the endoscopic detector;

If the narrow-band imaging mode is selected, the light source control unit controls the light source part to emit narrow-band light whose wavelength is not equal to the notch wavelength of the notch plate, and irradiates it on the biological tissue under inspection through the light guide channel, and the narrow-band light is reflected by the biological tissue. detected by an endoscopic detector;

If the fluorescence imaging mode is selected, the light source control unit controls the light source part to emit narrow-band light with a wavelength equal to the notch wavelength of the notch plate, which is irradiated on the biological tissue under inspection through the light guide channel, and the photosensitizer on the biological tissue under inspection is excited to generate fluorescence. , the narrow-band light reflected by the biological tissue to be inspected is irradiated to the endoscope part together with the fluorescence. After the reflected light is filtered by the notch filter, the fluorescence is detected by the endoscope detector.

Further, the white light-narrowband-fluorescence integrated endoscope includes a data acquisition part, and the data acquisition part includes an acquisition card and a memory card, and the acquisition card is used to receive the optical signal of the endoscope detector and the imaging mode signal of the light source control unit, and process it. Obtain the tissue image; the memory card is used to store the tissue image obtained by the acquisition card;

The acquisition card obtains an image label according to the imaging mode signal fed back by the light source control unit, obtains an image by processing according to the received light signal of the endoscope detector, and adds an image label to the image to obtain a tissue image.

Compared with the prior art, the present invention has the following beneficial effects:

In the endoscope of the present invention, the light source part can provide white light and narrow-band light of various wavelengths including fluorescent excitation light, that is, the complex narrow-band light splitting light path is placed at the light source end, not into the body, and can be expanded later as needed; The mirror part is equipped with a notch plate. When the illumination light is fluorescent excitation light, it can filter the reflected light of the fluorescent excitation light, and only allow the fluorescence to enter the endoscope detector. The endoscope part that enters the body is simplified, and the white light-narrow-band-fluorescence integrated endoscope design is realized without increasing the volume of the endoscope part through the optical path of small volume, so that the endoscope part of the present invention extending into the body is small in size, The structure is simple; the reflected light of the fluorescence excitation light in the fluorescence mode is filtered out by means of a notch, which solves the problem that the multi-spectral narrow-band function and the fluorescence imaging function are difficult to integrate. The invention realizes the integration of a white light endoscope, a multi-spectral narrow-band endoscope and a fluorescence endoscope. Whitespace. The invention has reasonable scheme, simple structure, easy realization, strong adaptability and wide application scenarios, and can be used for detecting oxygen content and photosensitizer concentration in the process of photodynamic therapy, thereby improving the integration degree of endoscope for deep tumor photodynamic therapy.

Further, the endoscope part of the present invention adopts an independently designed holder, which has an ingenious structure and low cost.

Further, the data acquisition part adopts an integrated acquisition card and memory card, which can perform both data acquisition and storage, and is compact in size.

The white-light-narrow-band-fluorescence integrated endoscope of the invention can realize simple switching between white-light imaging mode, narrow-band imaging mode and fluorescence imaging mode, and the same endoscope can be used to realize various detection modes.

Description of drawings

1 is a block diagram of the overall structure of the white light-narrowband-fluorescence endoscopic imaging system of the present invention;

FIG. 2 is a schematic structural diagram of an endoscope part of the present invention;

FIG. 3 is a schematic view of the structure of the gripper of the present invention;

4 is a schematic structural diagram of a light source part according to the first embodiment of the present invention;

5 is a schematic structural diagram of a light source part according to a second embodiment of the present invention;

Fig. 6 is the sequence gating flow chart of three modes of the present invention;

Light source part 100, endoscope part 200, data acquisition part 300, endoscope detector 210, notch plate 230, holder 240, light guide channel 241/242, endoscope detector channel 243, groove 244, xenon lamp light source 110. Focusing lens group 120, filter wheel 130, tunable liquid crystal filter LCTF 140.

Detailed ways

In order to further understand the present invention, the present invention will be described below in conjunction with the embodiments. These descriptions are only used to further explain the features and advantages of the present invention, and are not intended to limit the claims of the present invention.

The present invention is a white light-narrow-band-fluorescence endoscope imaging system, which realizes the integrated design of narrow-band reflection imaging and fluorescence imaging. The idea is to simplify the optical design of the endoscope part, separate the fluorescence and narrow-band light reflection bands through the notch, without increasing the volume of the endoscope part that needs to enter the body, and realize the excitation of white light-narrow-band light source in the external light source, so as to realize white-narrow-band-fluorescence. Targets for all-in-one endoscopy.

As shown in FIG. 1 , the white light-narrowband-fluorescence endoscopic imaging system of the present invention consists of three parts: a light source part 100 , a light source control unit, an endoscope part 200 , and a data acquisition part 300 .

The light source part 100 is used for providing illumination light irradiated on the biological tissue to be inspected, the type of illumination light includes white light and narrow-band light of various wavelengths, and the narrow-band light includes fluorescence excitation light; the fluorescence excitation light It can excite the photosensitizer on the tested biological tissue to generate fluorescence. The wavelength of the fluorescence excitation light needs to be selected according to the characteristics of the photosensitizer.

The light source control unit is used to control the type of illumination light provided by the light source part 100 .

The endoscope part 200 , which is a detection part extending into the biological tissue to be inspected, can capture and collect light signals from the tissue image of the biological tissue to be inspected under the illumination of the white light or narrow-band light provided by the light source part 100 .

The data acquisition part 300 receives the optical signal of the endoscope part 200 and the imaging mode signal of the light source control unit, and processes the tissue image to obtain and store the image.

As shown in FIG. 2 , the endoscope part 200 is composed of an endoscope detector 210 and a notch plate 230 , and the notch wavelength of the notch plate 230 is equal to the wavelength of the fluorescence excitation light. The endoscopic detector 210 and the notch plate 230 are fixed together by a holder 240, and the holder 240 is provided with a light guide channel, which can extend into human tissue for detection. The structure of the gripper 240 is shown in Figure 3. The structure is designed by SOLIDWORKS software and 3D printed with PLA material. This part adopts split printing and is divided into two parts: front and rear, with three channels in total. The two smaller diameter channels 241 and 242 on both sides are light guide channels, and the middle channel 243 with larger diameter is the endoscope detector channel. For installing the endoscopic detector 210 . There is a groove 244 at the front end of the endoscope detector channel (close to the biological tissue to be examined), which is used for fixing the notch plate 230 .

The endoscopic detector 210 is used to receive the reflected light of the illumination light provided by the light source part 100 and reflected by the biological tissue to be examined and the fluorescence generated by the excitation of the photosensitizer on the biological tissue to be examined by the fluorescence excitation light provided by the light source part 100 . When the light source part 100 provides the fluorescence excitation light, since the notch wavelength of the notch plate 230 is equal to the wavelength of the fluorescence excitation light, the notch filter can filter the reflected light of the biological tissue to be inspected, which can ensure that during fluorescence imaging, only Allow the fluorescence to reach the endoscopic detector.

The design of the holder 240 can ensure that the reflected light and the fluorescence are separated, and the purpose of filtering the reflected light of the tissue when realizing the fluorescence function imaging is guaranteed, which is the key link to ensure the integration of the fluorescence imaging function and the narrow-band imaging function.

The resolution of the endoscopic detector 210 is optionally 1920*1080.

The present invention can be used with a variety of different light sources, as long as the light sources can emit narrow-band light of different wavelengths. It can be matched with hyperspectral and hyperspectral light sources, broadening the application of hyperspectral and hyperspectral technologies, and combining multispectral, hyperspectral and even hyperspectral technologies with endoscopes, making up for the limited penetration of light, which is difficult to penetrate in the body. The shortcomings of the application.

As shown in FIG. 4 , in a feasible light source design solution, the light source part 100 uses a design solution of a xenon lamp light source 110 + a focusing lens group 120 + a filter wheel 130 . A focusing lens, a filter wheel 130 and a focusing lens are sequentially arranged in the direction of the emitted light of the xenon lamp light source 110 . The focusing lens can focus the light with a beam diameter of about 60mm emitted by the xenon lamp to a beam diameter of 4mm, and focus it on the light incident surface of the light guide channel. At the same time, the filter wheel is provided with through-holes that transmit white light and multiple filters of different wavelengths. The filters can select the required narrow-band waves from the wide-spectrum light emitted by the xenon lamp, so as to achieve the purpose of narrow-band imaging. , select the appropriate filter wheel according to your needs. As shown in FIG. 5 , in another feasible light source design solution, a design solution of xenon lamp light source 110 + focusing lens group 120 + tunable liquid crystal filter LCTF140 is used. Compared with filter wheels, LCTF can select more wavelengths and has higher controllability. These two possible light source designs can be used as examples of multispectral and hyperspectral light sources, respectively. Of course, the present invention does not limit the specific parameters and optical path structure of the light source, as long as it can emit white light and narrow-band light of different wavelengths, it can be used as a light source, such as a supercontinuum light source. In addition to the two feasible solutions listed above, instruments such as LEDs and spectrometers can also be used as light source components.

The data acquisition part 300 includes an acquisition card and a memory card, the acquisition card is used to receive the light signal of the endoscope detector 210 and the imaging mode signal of the light source control unit, and process the tissue image to obtain the tissue image; Storage; the acquisition card obtains the image label according to the imaging mode signal fed back by the light source control unit, and obtains the image according to the received optical signal processing of the endoscope detector 210, and adds the image label to the image to obtain the tissue image, which can be used for subsequent machine learning Algorithmic processing to reconstruct different functional images. For example, images acquired in narrow-band mode can be used to determine blood oxygen saturation, and images acquired in fluorescence mode can be used to determine the dose of photosensitizers before and after photodynamic therapy.

The data acquisition part adopts an integrated acquisition card and memory card, which is small in size. It is also equipped with an operating handle and a UDB peripheral connection slot, which can be connected to the peripheral USB to save images, which is easy to operate. The capture card has a real-time imaging function, which can be connected to an external monitor for real-time endoscopic image display. At the same time, the image can be frozen by operating the handle of the capture card, and the frozen image can be stored on an external USB device through a memory card.

The optical path of the invention has been simulated and verified by Zemax software, and the part of the optical path extending into the body is simple, small in size and wide in application scenarios, and can be used in the digestive tract and colorectum of small and medium-sized animals.

The working process of the system of the present invention is as follows: if it is in the white light imaging mode, the white light emitted by the light source part 100 is irradiated on the biological tissue to be inspected through the light guide channel, and the reflected light of the biological tissue to be inspected is detected by the endoscope detector to obtain a white light image, which is transmitted to the biological tissue. To the data acquisition part and displayed on the display, the white light image can provide the doctor with an intuitive feeling of the surgical site; in the narrow-band imaging mode, the narrow-band light emitted by the light source part 100 whose wavelength is not equal to the notch wavelength of the notch plate is irradiated through the light guide channel. On the inspected biological tissue, the reflected light of the inspected biological tissue is collected by the endoscope detector in the endoscope part 200, and the endoscope detector is connected with the data acquisition part 300, and can perform real-time display and storage of narrow-band images. The narrow-band image sent to the USB peripheral can generate multi-spectral or hyperspectral data sets, which can be combined with the corresponding algorithm to determine the blood oxygen concentration; in the fluorescence imaging mode, the wavelength emitted by the light source part 100 is equal to the notch wavelength of the notch plate. The narrow-band light (also the excitation light of the photosensitizer) is irradiated on the biological tissue to be inspected through the light guide channel, and the reflected light and the fluorescence of the biological tissue to be inspected are irradiated to the endoscope part 200 together. After the reflected light is filtered by the corresponding notch filter, Fluorescence is collected through the endoscope detector in the endoscope part 200, which is connected to the data acquisition part 300, and can display and store images in real time. The fluorescence mode can be used to detect the concentration of photosensitizers in the surgical site. The three modes of the endoscope do not need to be manually switched, but only need to be distinguished according to the wavelength of the illumination light used, and the wavelength of the illumination light of the light source part is controlled by the program. When the non-fluorescence excitation band is gated, The obtained image is marked as the narrow-band image of the corresponding band, which corresponds to the narrow-band imaging mode; when the fluorescence excitation band matching the notch wavelength of the notch filter is gated, because the excitation band is filtered out by the notch filter, the obtained is the fluorescence image, which corresponds to the fluorescence mode at this time. In short, it only needs to ensure that the excitation narrowband light wavelength of the fluorescence mode is exactly the notch wavelength of the notch plate. The timing gating flow chart of the three modes is shown in Figure 6.

It should be noted that the present invention does not limit the wavelength of the narrow-band light, and specific wavelength parameters need to be selected according to the optical characteristics of the biological tissue to be examined. Since the original intention of the present invention is to detect oxygen content and photosensitizer concentration in the process of measuring photodynamic therapy, the narrow-band light is preferably the central wavelength of which is located near the absorption peaks of blood oxyhemoglobin and deoxyhemoglobin, and the wavelength of the fluorescence excitation light is preferably light. Excitation wavelengths of common photosensitizers in dynamic therapy.

Claims (10)

1. A white light-narrow band-fluorescence integrated endoscope, comprising: a light source part (100), a light source control unit, and an endoscope part (200);

the light source part (100) is used for providing illumination light irradiated on the detected biological tissue, the type of the illumination light comprises white light and narrow-band light with multiple wavelengths, and the narrow-band light comprises fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be detected to generate fluorescence;

a light source control unit for controlling the type of illumination light provided by the light source section (100);

the endoscopic portion (200) includes an endoscopic probe (210) and a notch plate (230); the endoscope detector (210) is used for receiving reflected light of the illumination light provided by the light source part (100) after being reflected by the biological tissue to be detected and fluorescence generated by the excitation of the photosensitizer on the biological tissue to be detected by the fluorescence excitation light provided by the light source part (100) to obtain an optical signal, and the reflected light and the fluorescence enter the endoscope detector (210) through the notch film (230); the notch wavelength of the notch plate (230) is equal to the wavelength of the fluorescence excitation light.

2. The white light-narrow band-fluorescence integrated endoscope of claim 1, wherein the endoscope portion (200) further comprises a holder (240), an endoscope probe channel (243) is formed inside the holder (240), an endoscope probe (210) is installed in the endoscope probe channel (243), a groove (244) is formed in an end face of one end of the holder (240), the groove (244) is coaxial and communicated with the endoscope probe channel (243), and a wave trap (230) is installed in the groove (244).

3. The integrated endoscope of claim 2, characterized in that the holder (240) is provided with a light guide channel, and the axis of the light guide channel is parallel to the axis of the endoscope probe channel (243); the illumination light provided by the light source part (100) is irradiated on the biological tissue to be detected through the light guide beam channel.

4. The integrated white light-narrow band-fluorescence endoscope of claim 3, wherein two light guide beam channels are provided, and the two light guide beam channels are symmetrical with respect to the endoscope probe channel (243).

5. The integrated endoscope of claim 1, further comprising a data collection unit (300), wherein the data collection unit (300) comprises an acquisition card and a memory card, the acquisition card is used for receiving the optical signal of the endoscope detector (210) and the imaging mode signal of the light source control unit, and processing the optical signal and the imaging mode signal to obtain a tissue image; the memory card is used for storing the tissue images obtained by the acquisition card.

6. The integrated white light-narrow band-fluorescence endoscope of claim 5, further comprising a display for displaying the tissue image obtained by the acquisition portion (300) in real time.

7. The white light-narrow band-fluorescence integrated endoscope of claim 1, wherein the light source section (100) comprises a xenon lamp light source (110), a focusing lens group (120), and a filter wheel (130); the focusing lens group (120) comprises a first focusing lens and a second focusing lens; a first focusing lens, a filter wheel (130) and a second focusing lens are arranged in this order in the direction of light emitted from a xenon light source (110).

8. The integrated white-light-narrow-band-fluorescence endoscope of claim 1, characterized in that the light source section (100) comprises a xenon lamp light source (110), a focusing lens group (120) and a tunable liquid crystal filter LCTF; the focusing lens group (120) comprises a first focusing lens and a second focusing lens; the first focusing lens, the tunable liquid crystal filter LCTF and the second focusing lens are sequentially arranged in the emitting light direction of the xenon lamp light source (110).

9. The method for using the white light-narrow band-fluorescence integrated endoscope of any one of claims 1-8, which is characterized by comprising a white light imaging mode, a narrow band imaging mode and a fluorescence imaging mode;

if the white light imaging mode is selected, the light source control unit controls the light source part (100) to emit white light which is irradiated on the detected biological tissue through the light guide beam channel, and the reflected light of the white light reflected by the detected biological tissue is detected through the endoscope detector;

if the narrow-band imaging mode is selected, the light source control unit controls the light source part (100) to emit narrow-band light with the wavelength not equal to the trap wavelength of the trap sheet, the narrow-band light irradiates on the biological tissue to be detected through the light guide beam channel, and reflected light of the narrow-band light reflected by the biological tissue to be detected is detected through the endoscope detector;

if the fluorescence imaging mode is selected, the light source control unit controls the light source part (100) to emit narrow-band light with the wavelength equal to the notch wavelength of the notch plate, the narrow-band light is irradiated on the biological tissue to be detected through the light guide beam channel, the photosensitizer on the biological tissue to be detected is excited to generate fluorescence, the narrow-band light is irradiated on the endoscope part (200) through reflected light reflected by the biological tissue to be detected and the fluorescence together, the reflected light is filtered by the notch plate, and the fluorescence is detected through the endoscope detector.

10. The use method of the integrated endoscope comprises a data acquisition part (300), wherein the data acquisition part (300) comprises an acquisition card and a memory card, the acquisition card is used for receiving optical signals of the endoscopic probe (210) and imaging mode signals of the light source control unit, and processing the optical signals and the imaging mode signals to obtain a tissue image; the storage card is used for storing the tissue image obtained by the acquisition card;

the acquisition card obtains an image tag according to an imaging mode signal fed back by the light source control unit, obtains an image according to received optical signal processing of the endoscope detector (210), and adds the image tag to the image to obtain a tissue image.

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