CN114950580A - Micro droplet generation device - Google Patents

Abstract

The invention discloses a micro-droplet generating device, which comprises a generating chip, a first liquid sample collecting device and a second liquid sample collecting device, wherein the generating chip comprises a first compartment and a second compartment, and can generate liquid samples of the first compartment and the second compartment into micro-droplets; a collection plate comprising collection holes; connection base, connection base parcel collecting plate, connection base include the chip support, and it places and is spacing in the chip support to generate the chip, and collecting plate detachably installs on connection base. According to the micro-droplet generation device disclosed by the invention, the generation chip and the 96-well plate serving as the collection plate are integrated together through the connecting base, so that micro-droplets generated by the production chip can be directly collected by the 96-well plate serving as the collection plate. The collecting plate is detachably installed on the connecting base, and is convenient to directly disassemble after collecting the micro-droplets for subsequent operation without transferring the micro-droplets through other means and tools, so that the flow is simple and convenient, and the micro-droplet sample is not easily polluted in the process.

Description

Micro droplet generation device

technical field

The invention relates to the biological field, in particular to a microdroplet generating device.

Background technique

Micro-droplet technology is a micro-nano technology that utilizes the interaction between flow shear force and surface tension to divide and separate continuous fluids into discrete droplets of nanoscale and below volumes in microscale channels.

There are two main types of microdroplets: gas-liquid droplets and liquid-liquid droplets. Liquid-liquid microdroplets have the advantages of small size, no diffusion between droplet samples, avoidance of cross-contamination between samples, stable reaction conditions, and rapid mixing under proper control.

Liquid-liquid droplets are further classified into "oil-in-water", "water-in-oil", "oil-in-water-in-oil" and "water-in-oil-in-water" according to the difference of continuous phase and dispersed phase.

The micro-droplet generation system can generate "water-in-oil" or "oil-in-water" micro-droplets with a diameter in the micrometer scale (ie, 10-1000 μm), providing high sensitivity and high precision for many application scenarios in the fields of biology, chemistry and materials. An efficient and high-throughput research site.

The 96-well plate is a commonly used experimental consumable in the biological field, but the micro-droplet generation device in the prior art has poor compatibility with the 96-well plate, and cannot directly generate the micro-droplet sample into the 96-well plate. After the microdroplet generation device generates microdroplets, it is often necessary to transfer the microdroplets to a 96-well plate by other means and tools, which is complicated and prone to contamination of microdroplet samples during the process. Therefore, it is difficult to be used as an experimental system with high universality in the fields of biology, chemistry and materials.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is to overcome the poor compatibility between the micro-droplet generating device and the 96-well plate in the prior art, and it is impossible to directly generate the micro-droplet sample into the 96-well plate. After the droplet generation device generates microdroplets, it is often necessary to transfer the microdroplets to a 96-well plate by other means and tools, and the process is complicated and the contamination of the microdroplet samples is easy to occur during the process. A microdroplet generation device is provided. .

The present invention solves the above-mentioned technical problems through the following technical solutions:

A microdroplet generation device, comprising:

generating a chip, the generating chip comprising a first compartment and a second compartment, the generating chip can generate the liquid samples of the first compartment and the second compartment into microdroplets;

a collection plate, the collection plate is a 96-well plate, and the collection plate includes a collection hole;

a connection base, the connection base wraps the collection plate, the connection base includes a chip support, the generated chip is placed and limited to the chip support, and the collection plate is detachably mounted on the connection base seat.

In this solution, using the above structure, the generation chip is integrated with the 96-well plate as the collection plate through the connection base, so that the microdroplets generated by the production chip can be directly collected by the 96-well plate as the collection plate. The collection plate is detachably installed on the connection base, and it is also convenient to directly disassemble the micro-droplets for subsequent operations after collecting the micro-droplets. There is no need to transfer the micro-droplets by other means and tools. The process is simple and the micro-droplet samples are not easily contaminated during the process.

Preferably, the generation chip includes a sample discharge tube through which the microdroplets are discharged, and when the generation chip is placed and limited to the chip holder, the sample discharge tube extends into the collection hole.

In this solution, using the above structural form, through the limit of the chip holder, the sampling pipe can directly extend into the collection hole of the collection plate when the generation chip is placed on it, so that the generation chip and the collection plate are aligned. At the same time, the extension of the sampling tube into the collection hole can also reduce the height of the droplet droplet, reduce the kinetic energy of the droplet droplet, and avoid the impact of the droplet from damaging the droplet structure.

Preferably, the generated chip includes a plurality of chip modules, and the chip module includes a plurality of chip units corresponding to the collection holes one-to-one, and the plurality of chip units are integrally formed to form a chip module.

In this solution, using the above structure, the generated chip is composed of a plurality of identical chip modules, each chip module contains a plurality of chip units, and each chip unit can generate micro droplets independently. The number of chip units is related to the collection of The number of collecting holes of the plate corresponds one-to-one. Each individual chip module is integrally formed, which facilitates batch processing of the chip modules, and can reduce the production and maintenance costs of the generated chips.

Preferably, the chip module is formed of thermoplastic material or thermosetting material or glass.

In this solution, by adopting the above structure form, thermoplastic materials, thermosetting materials, glass and other materials are easy to process, and the cost is low, which further reduces the cost of generating chips.

Preferably, the generation chip includes:

a sample application layer, the sample application layer is arranged on the top of the generation chip, and the first compartment and the second compartment are arranged on the sample application layer;

The layout layer, the layout layer is arranged at the bottom of the generation chip, the layout tube is arranged on the layout layer, the top surface of the layout layer and the bottom surface of the sample application layer are attached to each other and combined seal;

A microfluidic layer, the top surface of the sample arrangement layer and the bottom surface of the sample application layer are adhered to each other and sealed to form the microfluidic layer, and the microfluidic layer includes the first compartment and the row A first flow channel communicated with the sample tube, a second flow channel communicated with the second compartment and the first flow channel, and the second flow channel is in the vertical direction of the first flow channel and the first flow channel. Generate area intersections.

In this solution, the above-mentioned structural form is adopted, the microfluidic layer is formed by bonding or gluing the sample application layer and the sample layout layer, and the three-layer structure of the chip is closely stacked and fitted, highly integrated, small in area and volume, and significantly reduced in size. cost of droplet generation.

Preferably, the top surface of the sample arrangement layer and/or the bottom surface of the sample application layer is recessed to form a first groove and a second groove, and the first groove and the second groove are connected with each other. The bottom surface of the sample application layer and/or the top surface of the sample layout layer are adhered to each other and sealed to form the first flow channel and the second flow channel.

In this solution, using the above structure, the generation chip generates micro droplets through the first flow channel and the second flow channel. The grooves on the bottom surface are formed. After the sample placement layer and the sample application layer are encapsulated by bonding or sticking, these grooves become the flow channels for the liquid samples to flow and intersect, producing micro droplets, making the chip highly integrated.

Preferably, the first flow channel includes in sequence along the flow direction of the liquid sample: a first inlet that communicates with the first compartment, a buffer area, a generation area, and an outlet that communicates with the sample discharge pipe;

The buffer area includes a first extended flow channel and a second extended flow channel extending along the side direction of the first flow channel, and the first extended flow channel and the second extended flow channel are communicated through a curved flow channel, so The first elongated flow passage communicates with the first inlet, and the second elongated flow passage communicates with the generation region.

In this solution, using the above structure, the liquid sample enters the first flow channel from the first inlet from the first compartment, and merges with the liquid sample in the second flow channel in the generation area to form droplets, and then self-discharges from the outlet Tube out. The buffer area is an extended section that is bent to one side and then returned to the original flow channel. A buffer area extended to one side is provided between the first inlet and the generation area, so that the liquid sample can be stored in the flow channel after entering the flow channel. Stabilize in the extended buffer area to make the flow rate and uniformity relatively stable, and then enter the production area to produce microdroplets, which can improve the generation effect of microdroplets.

Preferably, the first extended flow channel and the second extended flow channel are parallel to each other.

In this solution, using the above structure, the first extended flow channel and the second extended flow channel are parallel to each other, so that the distance between the two can be kept constant and the maximum distance can be maintained, so that the sample application layer and the sample layout layer can be bonded or It is easier to encapsulate by sticking and other methods, and the encapsulation effect is better.

Preferably, the sampling pipe includes:

an inner cavity, the cavity wall of the inner cavity gradually converges from the top of the inner cavity to the bottom of the inner cavity;

a liquid discharge port, the liquid discharge port is opened on one side of the bottom of the inner cavity;

A guide surface, the guide surface is arranged at the bottom of the inner cavity, one end of the guide surface is pressed against the cavity wall, and the other end of the guide surface is inclined downward and extends to the liquid discharge port.

In this scheme, the above-mentioned structural form is adopted, the sampling tube is connected to the outlet of the micro-channel layer, and the micro-droplets of the micro-channel layer flow out from the outlet and flow into the inner cavity of the sampling layer, and can flow along the cavity wall of the inner cavity to the inner cavity of the sampling layer. The bottom of the cavity flows to the guide surface, and then slowly flows from the guide surface to the liquid discharge port for discharge, which can avoid the direct drop of the droplet and the impact may damage the structure of the droplet.

Preferably, the sampling pipe further includes a drainage portion, the drainage portion is arranged on the cavity wall on the side where the liquid drainage port is opened in the inner cavity, and the drainage portion is convex along the cavity wall. The vertical projection of the distal end of the drainage portion is located on the drainage surface.

In this solution, the above-mentioned structure is adopted, and a convex drainage part is also provided above the cavity wall on the side where the liquid discharge port is opened in the sample discharge pipe, and the microdroplets on the side cavity wall can be guided to the cavity through the convex structure. On the guide surface, the micro-droplets are prevented from flowing directly from the liquid discharge port from the side cavity wall, and the guide effect of the sample discharge pipe is improved.

The positive improvement effect of the present invention is that: through the micro-droplet generating device disclosed in the present invention, the generating chip and the 96-well plate serving as the collecting plate are integrated together through the connection base, so that the micro-droplets generated by the manufacturing chip can be directly used as 96-well plate collection of the collection plate. The collection plate is detachably installed on the connection base, and it is also convenient to directly disassemble the micro-droplets for subsequent operations after collecting the micro-droplets. There is no need to transfer the micro-droplets by other means and tools. The process is simple and the micro-droplet samples are not easily contaminated during the process.

Description of drawings

FIG. 1 is a schematic structural diagram of a microdroplet generating device according to Embodiment 1 of the present invention.

FIG. 2 is a schematic diagram of the explosion structure of the microdroplet generating device according to Embodiment 1 of the present invention.

FIG. 3 is a partial structural schematic diagram of the connection base according to Embodiment 1 of the present invention.

FIG. 4 is a schematic structural diagram of a chip module according to Embodiment 1 of the present invention.

FIG. 5 is a schematic top-view structural diagram of the chip module according to Embodiment 1 of the present invention.

FIG. 6 is a schematic diagram of the connection structure between the chip module and the connection base according to Embodiment 1 of the present invention.

FIG. 7 is a schematic structural diagram of the microfluidic layer in Example 1 of the present invention.

FIG. 8 is a schematic structural diagram of the microfluidic layer of Example 1 of the present invention.

FIG. 9 is a schematic structural diagram of the microfluidic layer of Example 1 of the present invention.

FIG. 10 is a schematic structural diagram of the sample discharge tube in Example 2 of the present invention.

FIG. 11 is a schematic diagram of the internal structure of the sample discharge tube in Example 2 of the present invention.

Description of reference numbers:

Generate chip 1

Sample loading layer 11

Layout layer 12

Sampling tube 121

Exit 122

Lumen 123

Drain port 124

Guide surface 125

Drainage part 126

Chip module 13

Chip unit 14

first compartment 141

first entrance 143

Second entrance 144

Second compartment 142

Microfluidic layer 15

First runner 16

The first extension channel 161

The second extension channel 162

Bent runner 163

Second runner 17

Spawn area 18

connection base 2

Chip holder 21

Bump 211

Notch 212

Snap 22

collection plate 3

Collection hole 31

Card slot 32

Detailed ways

The present invention is further described below by way of examples, but the present invention is not limited to the scope of the described examples.

Example 1

As shown in FIGS. 1-3 , the microdroplet generation device of this embodiment includes a generation chip 1 , a collection plate 3 and a connection base 2 . The generation chip 1 includes a first compartment 141 and a second compartment 142 , and the generation chip 1 can generate the liquid samples in the first compartment 141 and the second compartment 142 into droplets. The collection plate 3 is a 96-well plate, and the collection plate 3 includes a collection hole 31 . The connecting base 2 wraps the collecting plate 3 , the connecting base 2 includes a chip support 21 , and the generated chip 1 is placed and limited to the chip support 21 , and the collecting plate 3 is detachably mounted on the connecting base 2 .

In this embodiment, the first compartment 141 and the second compartment 142 of the chip 1 are used for injecting the water phase liquid and the oil phase liquid respectively. The two compartments are respectively pressurized by the precise air control device, so that the liquid passes through the generating chip 1 to generate "water-in-oil" or "oil-in-water" droplets, which are discharged into the collection holes 31 of the collection plate 3 .

The collection plate 3 in this embodiment is a 96-well plate, which is a commonly used experimental consumable in the biological field. The plate has 8*12 collection holes 31 . In other embodiments, the collecting plate 3 can also be composed of several 8-connected tubes.

As shown in FIGS. 2 and 3 , the connection base 2 in this embodiment is a box type, and the connection base 2 can be sleeved on the collecting plate 3 and wrapped around the collecting plate 3 . The buckle 22 is detachably fixed to the card slot 32 on the collecting plate 3 . It makes the connection with the collecting plate 3 more stable. The card slots 32 are arranged on both sides of the collecting plate 3 , and the buckles 22 are arranged at corresponding positions on both sides of the connection base 2 . The buckle 22 is arranged on one end of a connecting plate that can be bent to a certain extent, the other end of the connecting plate is fixed on the side of the connection base 2, and the back of one end of the connecting plate where the buckle 22 is arranged is also provided with a handle portion. Extend to the other end of the connecting plate. By squeezing the handle portion, the connecting plate can be bent outwards, so that the buckle 22 is lifted up so as to be inserted into or disengaged from the locking groove 32 of the collecting plate 3 .

As shown in FIGS. 2 and 3 , the chip support 21 is composed of a plurality of sets of corresponding bumps 211 arranged at intervals on the tops of two opposite sides of the connection base 2 . When the chip is placed on the connection base 2 , the edges of the chip Then it is clamped in the notch 212 between the two protruding blocks 211 and aligned with the collecting plate 3 clamped on the connection base 2 .

In other embodiments, the connecting base 2 can also be connected to the collecting plate 3 in other ways without covering the collecting plate 3, for example, the upper frame structure can be installed on the top surface of the collecting plate 3, or the base structure can be made Put the collection plate 3 directly into the connection base 2 . The chip holder 21 is not limited to the bumps 211 and the notches 212 provided on the edge, and a top surface with a mounting groove is also added to the top of the connection base 2, or inside the connection base 2, the top of the collecting plate 3 is also used. It can be realized by adding a support structure on the surface, as long as the detachable installation of the collecting plate 3 and the fixing and limiting of the generation chip 1 can be realized.

The generation chip 1 is integrated with the 96-well plate as the collection plate 3 through the connection base 2 , so that the microdroplets generated by the production chip can be directly collected by the 96-well plate as the collection plate 3 . The collection plate 3 is detachably installed on the connection base 2, and it is also convenient to directly disassemble the micro-droplets for subsequent operations after collecting the micro-droplets. There is no need to transfer the micro-droplets by other means and tools. The process is simple and the micro-droplet samples are not easily contaminated during the process. .

As shown in FIG. 2 , the generation chip 1 includes a sample discharge pipe 121 through which the microdroplets are discharged. When the generation chip 1 is placed and limited to the chip holder 21 , the sample discharge pipe 121 extends into the collection hole 31 .

In this embodiment, the sample discharge pipe 121 is arranged on the bottom surface of the generation chip 1 and extends downward. When the generation chip 1 is clamped in the slot 212, the sample discharge pipe 121 of the generation chip 1 faces the collection port and extends into the into the collection hole 31.

Through the limitation of the chip holder 21 , when the generation chip 1 is placed on it, the sampling pipe 121 can directly extend into the collection hole 31 of the collection plate 3 , so that the generation chip 1 and the collection plate 3 are aligned. At the same time, the extension of the sampling tube 121 into the collection hole 31 can also reduce the drop height of the droplet, reduce the kinetic energy of droplet droplet, and avoid the droplet impact from damaging the droplet structure.

In other embodiments, the sample ejection port may only be aligned with the collection hole 31 without protruding into the collection hole 31, but this structure can only ensure that the droplets drop into the collection hole 31 but cannot reduce the droplet droplet kinetic energy.

As shown in FIGS. 1-5 , the generated chip 1 includes a plurality of chip modules, and the chip module includes a plurality of chip units 14 corresponding to the collection holes 31 one-to-one, and the plurality of chip units 14 are integrally formed to form a chip module.

In this embodiment, each chip unit 14 can generate micro droplets independently, including a set of first compartment 141 , second compartment 142 and sampling tube 121 , and a single chip module has 8 chips arranged laterally unit 14. The generation chip 1 of this embodiment is composed of 12 chip modules, and has 8*12 chip units 14 in total, corresponding to the 96-well plate serving as the collecting plate 3 .

In other embodiments, the arrangement direction and number of the chip units 14 of the chip module 13 are not limited to this, and can be adjusted according to different usage scenarios and different needs, as long as it can correspond to the collection holes 31 of the collection plate 3 Can.

The generation chip 1 is composed of a plurality of identical chip modules, and each chip module contains a plurality of chip units 14. Each chip unit 14 can generate micro droplets independently. The number of the chip units 14 is the same as the collection holes 31 of the collection plate 3 The numbers correspond one-to-one. Each individual chip module is integrally formed, which facilitates batch processing of the chip modules, and can reduce the production and maintenance costs of the generated chip 1 .

In this embodiment, the chip module is formed of thermoplastic material, thermosetting material, and glass. Such materials are easy to process and low in cost, which further reduces the cost of generating the chip 1 .

In other embodiments, other common thermoforming materials may also be used to form the product.

As shown in FIGS. 2-8 , the generation chip 1 includes a sample application layer 11 , a sample layout layer 12 and a microfluidic channel layer 15 . The sample adding layer 11 is arranged on the top of the generation chip 1, the first compartment 141 and the second compartment 142 are arranged on the sample adding layer 11; the sampling layer 12 is arranged on the bottom of the generating chip 1, and the sampling pipe 121 is arranged on the row On the sample layer 12, the top surface of the sample layout layer 12 and the bottom surface of the sample application layer 11 are adhered to each other and sealed; the top surface of the sample layout layer 12 and the bottom surface of the sample application layer 11 are adhered to each other and sealed to form a micro-channel layer 15. The layer 15 includes a first flow channel 16 in communication with the first compartment 141 and the sampling pipe 121 , a second flow channel 17 in communication with the second compartment 142 and the first flow channel 16 , and the second flow channel 17 along the first flow channel 16 The vertical direction of and the first flow channel 16 meet in the generation area 18 .

In this embodiment, the first compartment 141 communicates with the first flow channel 16 through the first inlet 143 , the second compartment 142 communicates with the second flow channel 17 through the second inlet 144 , and the second flow channel 17 is in the generation area. 18 communicates with the first flow channel 16 from both sides. The liquid in the two compartments enters the two flow channels under the action of air pressure and flows toward the outlet 122 . When the liquid in the first compartment 141 flows through the generation region 18 in the first flow channel 16 , corresponding droplets are generated under the action of the flow shear force of the liquid in the second flow channel 17 .

In this embodiment, the top surface of the layout layer 12 and the bottom surface of the sample application layer 11 are attached to each other and encapsulated by bonding or the like. In other embodiments, other common encapsulation methods such as glue sticking can also be used.

When the first compartment 141 is oil-phase liquid and the second compartment 142 is water-phase liquid, "oil-in-water" microdroplets can be generated;

When the first compartment 141 is water-phase liquid and the second compartment 142 is oil-phase liquid, "water-in-oil" microdroplets can be generated.

The microfluidic layer 15 in this embodiment is formed by the sample application layer 11 and the sample arrangement layer 12 by bonding or sticking. In other embodiments, an independent microfluidic channel layer 15 may also be provided and communicated with the first inlet 143 , the second inlet 144 and the outlet 122 of the sample application layer 11 and the sample arrangement layer 12 .

The microfluidic layer 15 is formed by the sample application layer 11 and the sample layout layer 12 by bonding or sticking. The three-layer structure of the chip is closely stacked, highly integrated, and small in area and volume, which significantly reduces the cost of microdroplet generation. .

As shown in FIG. 7 , the top surface of the sampling layer 12 and/or the bottom surface of the sampling layer 11 are recessed to form first grooves and second grooves. The bottom surface and/or the top surface of the layout layer 12 form the first flow channel 16 and the second flow channel 17 by bonding or sticking.

In this embodiment, both the first groove and the second groove are formed on the bottom surface of the sample application layer 11 . The layout layer 12 seals the grooves to form flow channels by bonding or gluing.

In other embodiments, the grooves can also be formed on the top surface of the alignment layer 12, or formed on both sides respectively.

The generation chip 1 generates micro droplets through the first flow channel 16 and the second flow channel 17 . Grooves are formed. After the sample placement layer 12 and the sample application layer 11 are encapsulated by bonding or gluing, these grooves become flow channels for liquid samples to flow and intersect, producing microdroplets, making the chip highly integrated.

As shown in FIG. 8 , the first flow channel 16 sequentially includes: a first inlet 143 communicating with the first compartment 141 , a buffer area, a generating area 18 , and an outlet 122 communicating with the sample discharge pipe 121 along the flow direction of the liquid sample. The buffer area includes a first extended flow channel 161 and a second extended flow channel 162 extending along the lateral direction of the first flow channel 16 . The flow channel 161 communicates with the first inlet 143 , and the second extended flow channel 162 communicates with the generation area 18 .

In this embodiment, the second flow channel 17 extends from the second outlet 122 from both sides respectively, and meets the first flow channel 16 from both sides of the first flow channel 16 in the vertical direction at the position of the generation area 18, and then It is then turned on to the outlet 122 . The first flow channel 16 first passes through a buffer area extended by bending from the position of the first inlet 143 , then extends to the generating area 18 where it meets the second flow channel 17 , and then leads to the position of the outlet 122 .

As shown in FIG. 8 , the buffer area of this embodiment consists of a first extended flow channel 161 that communicates with the first inlet 143 and extends laterally, a bent flow channel 163 that communicates with the rear end of the first extended flow channel 161 and is bent longitudinally, and The second extended flow channel 162 is formed by connecting the tail end of the bent flow channel 163 and extending in the opposite direction of the first extended flow channel 161 in the transverse direction. The entire buffer area is in a spiral structure.

In other embodiments, the buffer area can also be provided with more extended flow channels and bent flow channels 163 to form a multi-layer spiral structure to further extend the overall length of the buffer area and increase its flow stabilization effect. However, under the condition that the total length remains unchanged, using only one buffer area of the bending structure can make the distance between the two extended flow channels the longest, and can reduce the bonding or adhesive bonding between the sample application layer 11 and the sample layout layer 12. The difficulty of packaging.

The liquid sample enters the first flow channel 16 from the first inlet 143 from the first compartment 141 , and merges with the liquid sample in the second flow channel 17 in the generation area 18 to form droplets, and then flows out from the sample discharge tube 121 through the outlet 122 . The buffer area is an extended section that is bent to one side and then returned to the original flow channel direction. A buffer area extended to one side is provided between the first inlet 143 and the generation area 18, so that the liquid sample can enter the flow channel after entering the flow channel. It can be stabilized in the extended buffer area to make the flow rate and uniformity relatively stable, and then enter the production area to produce micro-droplets, which can improve the generation effect of micro-droplets.

As shown in FIG. 8 , the first extended flow channel 161 and the second extended flow channel 162 are parallel to each other.

The first extended flow channel 161 and the second extended flow channel 162 are parallel to each other, so that the distance between the two can be kept constant and the maximum distance can be maintained, so that the sample application layer 11 and the sample layout layer 12 can be packaged by bonding or sticking. Easier and better encapsulation.

As shown in FIG. 9 , the sample discharge tube 121 of this embodiment includes an inner cavity 123 and a liquid discharge port 124 , and the cavity wall of the inner cavity 123 gradually converges from the top of the inner cavity 123 to the bottom of the inner cavity 123 . The drain port 124 is opened at the bottom of the inner cavity 123 .

The inside of the sampling tube 121 is hollow to form an inner cavity 123 , the top of the inner cavity 123 is connected to the outlet 122 of the microfluidic layer 15 , and the microdroplets formed by the microfluidic layer 15 flow downward from the outlet 122 along the cavity wall of the inner cavity 123 . And drip from the liquid drain 124 at the bottom of the inner cavity 123 into the collection hole 31 of the collection plate 3 .

Embodiment 2

The structure of the micro-droplet generating device of this embodiment is substantially the same as that of the first embodiment, and the same parts are not repeated here, and the difference lies in the sample discharge pipe 121 of this embodiment.

As shown in FIGS. 10 and 11 , the sample discharge tube 121 includes an inner cavity 123 , a liquid discharge port 124 and a guide surface 125 . The cavity wall of the inner cavity 123 gradually converges from the top of the inner cavity 123 to the bottom of the inner cavity 123 . The drain port 124 is opened on one side of the bottom of the inner cavity 123 . The guide surface 125 is disposed at the bottom of the inner cavity 123 , one end of the guide surface 125 is pressed against the cavity wall, and the other end of the guide surface 125 is inclined downward and extends to the liquid discharge port 124 .

In this embodiment, the sample discharge tube 121 is hollow inside to form an inner cavity 123 , the top of the inner cavity 123 is connected to the outlet 122 of the microfluidic layer 15 , and the microdroplets formed by the microfluidic layer 15 flow from the outlet 122 along the inner cavity 123 . The cavity wall flows downward. The liquid discharge port 124 is opened at the bottom of the side surface of the sample discharge tube 121 , and the guide surface 125 is provided on the bottom of the inner cavity 123 along the cavity wall of the inner cavity 123 , which slopes downward toward the liquid discharge port 124 .

The micro droplets can flow along the cavity wall of the inner cavity 123 to the bottom of the cavity to the guide surface 125, and then slowly flow from the guide surface 125 to the liquid discharge port 124 to be discharged, which can avoid the direct drop of the micro droplets and the impact of excessive damage may be damaged. Microdroplet structure.

As shown in FIGS. 10 and 11 , the sample discharge tube 121 further includes a drainage portion 126 . The drainage portion 126 is arranged on the cavity wall on the side where the liquid discharge port 124 is opened in the inner cavity 123 . The drainage portion 126 protrudes along the cavity wall and discharges The liquid port 124 extends, and the projection of the end of the drainage portion 126 in the vertical direction is located on the drainage surface 125 .

In this embodiment, the inclination angle of the drainage part 126 is slightly larger than the inclination angle of the cavity wall, and its end is located on the guiding surface 125 in the vertical direction, so as to ensure that the droplets dropped from the drainage part 126 can land on the guiding surface 125, to prevent the micro-droplets from directly flowing out of the liquid discharge port 124 from the side cavity wall, and improve the diversion effect of the sample discharge pipe 121.

Although the specific embodiments of the present invention are described above, those skilled in the art should understand that this is only an illustration, and the protection scope of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principle and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims (11)

1. A micro-droplet generator, comprising:

a generation chip comprising a first compartment and a second compartment, the generation chip being capable of generating liquid samples of the first compartment and the second compartment as microdroplets;

a collection plate comprising collection wells;

the connection base wraps the collecting plate, the connection base comprises a chip support, the generated chips are placed and limited on the chip support, and the collecting plate is detachably mounted on the connection base.

2. The micro-droplet generation apparatus of claim 1, wherein the collection plate is a 96-well plate.

3. The micro-droplet generating device of claim 1, wherein the generating chip comprises a sampling tube through which the micro-droplets are discharged, and wherein the sampling tube extends into the collecting hole when the generating chip is placed on and retained by the chip holder.

4. The micro-droplet generator of claim 1, wherein the generating chip comprises a plurality of chip modules, each chip module comprises a plurality of chip units corresponding to the collecting holes, and the plurality of chip units are integrally formed to form the chip module.

5. The microdroplet generating device of claim 4, wherein the chip module is formed from a thermoplastic material or a thermoset material or a glass material.

6. A microdroplet generating device as claimed in claim 3, wherein the generating chip comprises:

the sample adding layer is arranged on the top of the generated chip, and the first compartment and the second compartment are arranged on the sample adding layer;

the sample arrangement layer is arranged at the bottom of the generated chip, the sample arrangement pipe is arranged on the sample arrangement layer, and the top surface of the sample arrangement layer and the bottom surface of the sample adding layer are mutually attached and sealed;

the micro flow channel layer, the drainage layer top surface with the bottom surface on application of sample layer pastes each other and sealed formation the micro flow channel layer, the micro flow channel layer include with first compartment with the first runner of sample discharge pipe UNICOM, with the second compartment with the second runner of first runner intercommunication, the second runner is followed the vertical direction of first runner with first runner is at the regional intersection that generates.

7. The micro-droplet generating device according to claim 6, wherein the top surface of the sample application layer and/or the bottom surface of the sample application layer are recessed to form a first groove and a second groove, and the first groove and the second groove form the first flow channel and the second flow channel by being attached to and sealed with the bottom surface of the sample application layer and/or the top surface of the sample application layer.

8. The micro-droplet generating apparatus of claim 6, wherein the first flow channel comprises, in order along the flow direction of the liquid sample: a first inlet communicated with the first compartment, the buffer area, the generation area and an outlet communicated with the sampling pipe;

the buffer area comprises a first extension flow channel and a second extension flow channel which extend along the side face direction of the first flow channel, the first extension flow channel is communicated with the second extension flow channel through a bent flow channel, the first extension flow channel is communicated with the first inlet, and the second extension flow channel is communicated with the generation area.

9. The apparatus of claim 8, wherein the first elongated flow channel and the second elongated flow channel are parallel to each other.

10. A microdroplet generating device as claimed in claim 3, wherein the drainage tube comprises:

the cavity wall of the inner cavity gradually converges from the top of the inner cavity to the bottom of the inner cavity;

the liquid discharge port is formed in one side of the bottom of the inner cavity;

the flow guide surface is arranged at the bottom of the inner cavity, and the flow guide surface inclines downwards from the cavity wall position and extends to the liquid outlet.

11. The apparatus as claimed in claim 10, wherein the sampling tube further comprises a drainage portion, the drainage portion is disposed on the cavity wall at a side of the inner cavity where the liquid outlet is disposed, the drainage portion protrudes along the cavity wall and extends toward the liquid outlet, and a projection of a distal end of the drainage portion in a vertical direction is located on the flow guide surface.

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