CN115124731A - A kind of preparation method of high-valued legume interface super-assembled SAFs fluorescent material - Google Patents

Abstract

The invention provides a method for preparing a high-valued legume interface super-assembled SAFs fluorescent material. First, the germinated fresh legume is placed under simulated sunlight and incubated in a disodium terephthalate solution to obtain an incubation method. Then transfer the incubated legumes to the aqueous solution of lanthanide metals to continue incubation, and freeze-dry to obtain high-valued legume interface super-assembled SAFs fluorescent material, wherein the legume is mung bean sprouts , any one of soybean sprouts, broad bean sprouts, pea sprouts, red bean sprouts, mung bean sprouts and cowpea sprouts, and the lanthanide metal is europium chloride hexahydrate or terbium chloride hexahydrate. The preparation method has the advantages of simple process, high efficiency, wide source of raw materials, environmental friendliness, strong sustainability, and can realize large-scale production. In addition, the preparation method reduces manual intervention, and the SAFs with fluorescent properties are synthesized by self-superassembly in plants.

Description

A preparation method of high-valued legume interface super-assembled SAFs fluorescent material

technical field

The invention belongs to the technical field of biological materials, and in particular relates to a preparation method of a high-valued legume interface super-assembled SAFs fluorescent material.

Background technique

Legumes are the third largest family after Compositae and Orchidaceae in the class of Dicotyledons. They have trees, shrubs or herbs, erect or climbing forms, rich in variety and complexity, and have strong adaptability to the living environment. The distribution of legumes can be found in mountains, grasslands, forests and even deserts. The origin of leguminous plants has a long history. With the evolution of human consciousness and thought, the development and utilization of leguminous plants has gradually diversified, from the original simple cultivation to the later production and processing, from rough to delicate processing technology, and single variety to Diversification, the whole series of processes continue to evolve and sublimate. However, as a green and environmentally friendly material, plant woven material is not widely used in modern society due to the limited functionality of plants and the complexity of the processing process.

Metal-Organic Frameworks (MOFs) refer to crystalline porous materials with periodic network structure formed by self-assembly of transition metal ions and organic ligands. It has the advantages of high porosity, low density, large specific surface area, regular pore channels, adjustable pore size, and topology diversity and tailorability. Therefore, the huge potential applications in heterogeneous catalysis, molecular recognition, gas storage, ion exchange, functional materials, etc. have become the current research hotspot. Among them, lanthanide ions have unique optical and magnetic properties, and MOFs containing lanthanide ions have also received extensive attention from scientific and technological workers.

Plant transpiration is the process by which water evaporates from the surface of a plant's living body. The amount of water lost by transpiration during the whole growth period of plants accounts for 50-60% of the total water consumption. The pulling force generated by transpiration is the driving force for water absorption, and is the driving force for the transportation of water and inorganic salts in plants. However, the effective utilization of plant transpiration to efficiently and simply prepare super-assembled materials in plants will be a key challenge to expand the functionality of plant woven materials.

SUMMARY OF THE INVENTION

In order to solve the problems of the prior art, the present invention provides a preparation method of a high-valued legume interface super-assembled SAFs fluorescent material.

The concrete technical scheme of the present invention is as follows:

The present invention provides a method for preparing a high-valued legume interface super-assembled SAFs fluorescent material, which is characterized by comprising the following steps: Step S1, placing the germinated fresh legume under simulated sunlight, and in para-benzene Incubate in disodium diformate solution to obtain incubated legumes; step S2, transfer the incubated legumes to an aqueous solution of lanthanide metals to continue incubation; step S3, freeze-dry to obtain high-value legumes Plant interface super-assembled SAFs fluorescent material, wherein the legume is any one of mung bean sprouts, soybean sprouts, broad bean sprouts, pea sprouts, red bean sprouts, mung bean sprouts and cowpea sprouts, and the lanthanide metal is europium chloride hexahydrate or Terbium chloride hexahydrate.

The preparation method of the high-valued legume interface super-assembled SAFs fluorescent material provided by the present invention also has the following technical features, wherein the concentration of the disodium terephthalate solution in step S1 is 1-300 mmol/L, and the dosage is 0.5-50 mL, the preferred concentration is 10-250 mmol/L, and the preferred dosage is 15-25 mL.

The preparation method of the high-valued legume interface super-assembled SAFs fluorescent material provided by the present invention also has the following technical characteristics, wherein in step S2, the concentration of the aqueous solution of the lanthanide metal is 1-300 mmol/L, and the dosage is 0.4-300 mmol/L. 50mL, the preferred concentration is 10-250mmol/L, and the preferred dosage is 15-25mL.

The method for preparing the high-valued legume interface super-assembled SAFs fluorescent material provided by the present invention also has the technical feature that the simulated sunlight in step S1 is generated by a high-power sunlight simulator.

The preparation method of the high-valued legume interface super-assembled SAFs fluorescent material provided by the present invention also has the following technical characteristics, wherein the power of the high-power sunlight simulator is 100-2000W, preferably 200-500W.

The preparation method of the high-valued legume interface super-assembled SAFs fluorescent material provided by the present invention also has the following technical characteristics, wherein the incubation time in the disodium terephthalate solution in step S1 is 2 to 72 hours, preferably The time is 42-54h.

The preparation method of the high-valued legume interface super-assembled SAFs fluorescent material provided by the present invention also has the following technical characteristics, wherein in step S2, the time for continuous incubation in the aqueous solution of the lanthanide metal is 2 to 72 hours, and the preferred time is 2-72 hours. 42～54h.

The invention also provides a high-valued legume interface super-assembled SAFs fluorescent material, which is characterized in that it is prepared by using the above-mentioned preparation method of the high-value legume interface super-assembled SAFs fluorescent material.

The role and effect of the invention

In the present invention, the germinated fresh legumes are firstly placed under simulated sunlight and incubated in a disodium terephthalate solution to obtain the incubated legumes; then the incubated legumes are transferred to a lanthanide metal Continue to incubate in aqueous solution and freeze-dry to obtain high-valued legume interface super-assembled SAFs fluorescent material, wherein legumes are mung bean sprouts, soybean sprouts, broad bean sprouts, pea sprouts, red bean sprouts, mung bean sprouts and cowpea sprouts. Either way, the lanthanide metal is europium chloride hexahydrate or terbium chloride hexahydrate.

Therefore, compared with the prior art, the preparation method of the high-valued legume interface super-assembled SAFs fluorescent material provided by the present invention is simple and efficient, has a wide range of raw material sources, is environmentally friendly, has strong sustainability, and can realize large-scale production. . In addition, the preparation method reduces manual intervention, and the SAFs with fluorescent properties are synthesized by self-superassembly in plants.

Description of drawings

Figure 1 is an optical photograph of the preparation of mung bean sprouts in vivo interface super-assembled SAFs fluorescent material in Example 1.

FIG. 2 is an optical image of the super-assembled SAFs fluorescent material in the in vivo interface of mung bean sprouts prepared in Example 1 under 245 nm fluorescence and fluorescent light.

3 is a SEM cross-sectional view of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1. FIG. FIG. 4 is an enlarged SEM cross-sectional view of the super-assembled SAFs fluorescent material in the mung bean sprouts in vivo interface prepared in Example 1. FIG.

5 is a SEM longitudinal cross-sectional view of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1. FIG.

FIG. 6 is an enlarged view of the SEM longitudinal section of the super-assembled SAFs fluorescent material in the mung bean sprouts in vivo interface prepared in Example 1. FIG.

7 is an inverted fluorescence microscope cross-sectional view of the in vivo interface super-assembled SAFs fluorescent material of the mung bean sprouts prepared in Example 1. FIG.

8 is an enlarged view of the cross-section of the super-assembled SAFs fluorescent material of the mung bean sprouts in vivo interface prepared in Example 1 by an inverted fluorescence microscope.

9 is a vertical cross-sectional view of an inverted fluorescence microscope of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1. FIG.

10 is an enlarged view of the longitudinal section of the mung bean sprouts in vivo interface super-assembled SAFs fluorescent material prepared in Example 1 by an inverted fluorescence microscope.

11 is a fluorescent lamp image of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1 after freeze-drying and bending.

12 is a 245 nm fluorescence image of the super-assembled SAFs fluorescent material in the in vivo interface of mung bean sprouts prepared in Example 1 after freeze-drying and bending.

Figure 13 is an optical photograph of the preparation of Example 2 when the SAFs fluorescent material was super-assembled at the interface in the soybean sprout.

Figure 14 is a SEM cross-sectional view of blank soybean sprouts.

15 is a SEM cross-sectional view of the super-assembled SAFs fluorescent material in the soybean sprout prepared in Example 2.

16 is a fluorescent optical photograph of the super-assembled SAFs fluorescent material in the soybean sprout prepared in Example 2.

Detailed ways

Terms used in the present invention generally have the meanings commonly understood by those of ordinary skill in the art unless otherwise specified.

In the following examples, various procedures and methods not described in detail are conventional methods well known in the art.

The reagents used in the following examples are purchased from common commercial channels, and the unremarked experimental operations and experimental conditions refer to the conventional operations and conventional conditions in the art.

The mung bean sprouts used in the following examples are formed from the germination of mung bean for four days, and the soybean sprouts are formed from the germination of soybeans for four days. The experimental drugs were purchased from aladdin company, disodium terephthalate CAS number: 10028-70-3, molecular formula: C ₈ H ₄ Na ₂ O ₄ , molecular weight: 210.1; Europium chloride hexahydrate CAS number: 13759-92- 7. Molecular formula: EuCl ₃ ·6H ₂ O, molecular weight: 366.41; Terbium chloride hexahydrate CAS number: 13798-24-8, molecular formula: TbCl ₃ ·6H ₂ O, molecular weight: 373.38; WINSURE brand high-power sunlight simulator.

The specific embodiments of the present invention will be described below with reference to the embodiments and the accompanying drawings.

<Example 1>

The present embodiment provides a method for preparing a Mung bean sprout body interface super-assembled SAFs fluorescent material, comprising the following steps:

In step S1, the germinated fresh legumes are placed under simulated sunlight and incubated in a disodium terephthalate solution to obtain the incubated legumes. The specific process is as follows:

Add 840 mg of disodium terephthalate to 20 mL of deionized water, stir for 30 minutes, then place the germinated fresh mung bean sprouts upright, and incubate for 48 hours under the irradiation of a high-power sunlight simulator (power of 300 W) to obtain incubation after the live mung bean sprouts;

In step S2, the incubated legume is transferred to an aqueous solution of lanthanide metals to continue incubation, and the specific process is as follows:

Add 147 mg of terbium chloride hexahydrate to 20 mL of deionized water, stir for 30 minutes, and then immerse the incubated live mung bean sprouts after washing with deionized water, and continue to irradiate under the high-power sunlight simulator (power of 300W). Incubate for 48h;

Step S3, freeze-drying, to obtain a high-valued legume interface super-assembled SAFs fluorescent material. The specific process is as follows:

Rinse with deionized water and freeze-dry in a freeze dryer to obtain the super-assembled SAFs fluorescent material in the in vivo interface of mung bean sprouts.

Figure 1 is an optical photograph of the preparation of mung bean sprouts in vivo interface super-assembled SAFs fluorescent material in Example 1.

SEM test characterization and fluorescence photography were carried out on the Mung bean sprouts in vivo interface super-assembled SAFs fluorescent material prepared in this example. The result is as follows:

FIG. 2 is an optical image of the super-assembled SAFs fluorescent material in the in vivo interface of mung bean sprouts prepared in Example 1 under 245 nm fluorescence and fluorescent light. It can be seen from FIG. 2 that the Mung bean sprouts in-vivo interface super-assembled SAFs fluorescent material prepared in this example indeed has fluorescent light-emitting properties at 245 nm.

3 is a SEM cross-sectional view of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1. FIG. FIG. 4 is an enlarged SEM cross-sectional view of the super-assembled SAFs fluorescent material in the mung bean sprouts in vivo interface prepared in Example 1. FIG. 5 is a SEM longitudinal cross-sectional view of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1. FIG. FIG. 6 is an enlarged view of the SEM longitudinal section of the super-assembled SAFs fluorescent material in the mung bean sprouts in vivo interface prepared in Example 1. FIG. It can be seen from Figures 3, 4, 5 and 6 that the particles with SAFs structure grow in mung bean sprouts, indicating that SAFs are synthesized by interface superassembly in mung bean sprouts.

7 is an inverted fluorescence microscope cross-sectional view of the in vivo interface super-assembled SAFs fluorescent material of the mung bean sprouts prepared in Example 1. FIG. FIG. 8 is an enlarged view of the cross-sectional view of the super-assembled SAFs fluorescent material of the mung bean sprouts in vivo interface prepared in Example 1 by an inverted fluorescence microscope. 9 is a vertical cross-sectional view of an inverted fluorescence microscope of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1. FIG. 10 is an enlarged view of the longitudinal section of the mung bean sprouts in vivo interface super-assembled SAFs fluorescent material prepared in Example 1 by an inverted fluorescence microscope. It can be seen from Figures 7, 8, 9 and 10 that the SAFs particles in the mung bean sprouts show fluorescence, indicating that the fluorescent SAFs are synthesized by the interface super-assembly in the mung bean sprouts.

11 is a fluorescent lamp image of the super-assembled SAFs fluorescent material in the in vivo interface of the mung bean sprouts prepared in Example 1 after freeze-drying and bending. 12 is a 245 nm fluorescence image of the super-assembled SAFs fluorescent material in the in vivo interface of mung bean sprouts prepared in Example 1 after freeze-drying and bending. It can be seen from Figures 11 and 12 that the super-assembled SAFs fluorescent material of the mung bean sprouts in vivo interface prepared in this example indeed has fluorescence emission properties at 245 nm.

<Example 2>

The present embodiment provides a method for preparing a fluorescent material for super-assembled SAFs at the interface of soybean sprouts, including the following steps:

In step S1, the germinated fresh legumes are placed under simulated sunlight and incubated in a disodium terephthalate solution to obtain the incubated legumes. The specific process is as follows:

Add 840 mg of disodium terephthalate to 20 mL of deionized water, stir for 30 min, then put the germinated fresh soybean sprouts upright, and incubate for 48 h under the irradiation of a high-power sunlight simulator (power of 300 W) to obtain incubation post-living soybean sprouts;

In step S2, the incubated legume is transferred to an aqueous solution of lanthanide metals to continue incubation, and the specific process is as follows:

Add 149 mg of europium chloride hexahydrate to 20 mL of deionized water, stir for 30 minutes, and then immerse the incubated live soybean sprouts after washing with deionized water, and continue to irradiate under the high-power sunlight simulator (power of 300W). Incubate for 48h;

Step S3, freeze-drying, to obtain a high-valued legume interface super-assembled SAFs fluorescent material. The specific process is as follows:

Rinse with deionized water and freeze-dry in a freeze-drying machine to obtain the super-assembled SAFs fluorescent material within the soybean sprout.

Figure 13 is an optical photograph of the preparation of Example 2 when the SAFs fluorescent material was super-assembled at the interface in the soybean sprout.

SEM test and characterization and fluorescence photography were carried out on the super-assembled SAFs fluorescent materials in the soybean sprouts prepared in this example. The germinated fresh soybean sprouts were rinsed with deionized water, and then freeze-dried in a freeze dryer to obtain blank soybean sprouts. The blank soybean sprouts were characterized by SEM test. The result is as follows:

Figure 14 is a SEM cross-sectional view of blank soybean sprouts. 15 is a SEM cross-sectional view of the super-assembled SAFs fluorescent material in the soybean sprout prepared in Example 2. It can be seen from Figures 14 and 15 that the interfacial superassembly of the soybean sprouts undergoes interfacial superassembly to synthesize granular SAFs.

16 is a fluorescent optical photograph of the super-assembled SAFs fluorescent material in the soybean sprout prepared in Example 2. It can be seen from Fig. 16 that the SAFs fluorescent material can be clearly observed with the naked eye and grow inside the legumes with the plants.

The above is a detailed description of the embodiments, which is convenient for those skilled in the art to correctly understand and use the present invention. Any improvement or modification of technical solutions obtained by those skilled in the art on the basis of the prior art according to the present invention, without innovative labor, but only through analysis, analogy or limited enumeration, should be determined by the claims. within the scope of protection.

Claims (8)

1. A preparation method of a high-valued legume interface super-assembly SAFs fluorescent material is characterized by comprising the following steps:

step S1, placing the germinated fresh leguminous plants in simulated sunlight, and incubating in a disodium terephthalate solution to obtain incubated leguminous plants;

step S2, transferring the incubated leguminous plants into an aqueous solution of lanthanide metals for continuous incubation;

step S3, freeze-drying to obtain the high-value super-assembled SAFs fluorescent material on the leguminous plant interface,

wherein the leguminous plant is any one of mung bean sprout, soybean sprout, broad bean sprout, pea bean sprout, red bean sprout, mung bean sprout and cowpea sprout,

the lanthanide metal is europium chloride hexahydrate or terbium chloride hexahydrate.

2. The method for preparing high-valued legume interface super-assembled SAFs fluorescent material according to claim 1,

wherein, the concentration of the disodium terephthalate solution in the step S1 is 1-300 mmol/L, and the dosage is 0.5-50 mL.

3. The method for preparing high-valued legume interface super-assembled SAFs fluorescent material according to claim 1,

wherein, the concentration of the aqueous solution of lanthanide metal in step S2 is 1-300 mmol/L, and the dosage is 0.4-50 mL.

4. The method for preparing high-valued legume interface super assembly SAFs fluorescent material according to claim 1,

wherein the simulated sunlight is generated by the high-power sunlight simulator in step S1.

5. The method for preparing high-valued legume interface super-assembled SAFs fluorescent material according to claim 4,

the power of the high-power solar simulator is 100-2000W.

6. The method for preparing high-valued legume interface super-assembled SAFs fluorescent material according to claim 1,

wherein the incubation time in the disodium terephthalate solution in the step S1 is 2-72 h.

7. The method for preparing high-valued legume interface super-assembled SAFs fluorescent material according to claim 1,

wherein the continuous incubation time in the lanthanide metal aqueous solution in the step S2 is 2-72 h.

8. The high-value leguminous plant interface super-assembly SAFs fluorescent material is prepared by the preparation method of the high-value leguminous plant interface super-assembly SAFs fluorescent material as claimed in any one of claims 1 to 7.

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