CN115228633A - A kind of circulating centrifugal separation impurity system and its chromatography system - Google Patents

Abstract

The invention discloses a circulating centrifugal separation impurity system and a chromatography system thereof. The impurity separation system includes a buffer tank, the top of the buffer tank is respectively connected with a conical bottom outlet of a first cyclone separator and a columnar top outlet of a second cyclone separator, and the bottom of the buffer tank is connected to The middle inlet of the first cyclone separator, the middle inlet of the second cyclone separator and a drain pipe are respectively connected, wherein the cylindrical top outlet of the first cyclone separator is connected to a first cyclone. A waste discharge pipeline, the conical bottom outlet of the second cyclone is connected to a second waste discharge pipeline. The circulatory centrifugal separation impurity system and its chromatographic system in the present invention do not need to wash the sieve, that is, rapid separation can be achieved, thereby improving the efficiency of chromatography.

Description

A kind of circulating centrifugal separation impurity system and its chromatography system

technical field

The invention relates to the field of chromatography systems, in particular to a circulating centrifugal separation impurity system and a chromatography system thereof.

Background technique

The patent No. US20060130444A1 in the prior art introduces a separation device with multiple hydrocyclones connected in series, wherein the overflow pipe of each separation stage is connected to the inlet of the next separation stage. The patent publication number CN208912333U relates to a multi-stage and multi-stage hydrocyclone separation device. Through at least two first-stage hydrocyclone separators connected in series, the first outlets of the two-stage hydrocyclone separators are respectively connected to the corresponding The first outlet of the first-stage hydrocyclone separator and the second outlet of the second-stage hydrocyclone separator are connected to a sedimentation separator, which has the characteristics of good separation effect and large processing capacity, but its disadvantage is that the separation material is The hearing time in the device is long, and rapid separation cannot be achieved.

At the same time, during the chromatography process, the stock solution enters directly from the lower mobile phase and exits from the upper mobile phase, or the lower mobile phase exits and enters from the upper mobile phase. Due to the presence of a large amount of impurities in the stock solution, the upper and lower sieves will be blocked. The screen needs to be washed, and the frequency of washing will increase, which will lead to the progress of the chromatographic separation, which will affect the efficiency.

SUMMARY OF THE INVENTION

In order to solve the above problems, the present invention provides a circulating centrifugal separation system for impurities and a chromatography system thereof.

According to one aspect of the present invention, there is provided a circulating centrifugal separation system for impurities, comprising a buffer tank, the top of which is respectively connected to a conical bottom outlet of a first cyclone and a second cyclone The cylindrical top outlet of the buffer tank is connected to the middle inlet of the first cyclone separator, the middle inlet of the second cyclone separator and a drain pipe respectively, wherein the first cyclone separator is The cylindrical top outlet of a cyclone is connected to a first waste discharge pipe, and the conical bottom outlet of the second cyclone is connected to a second waste discharge pipe.

In some embodiments, the top of the buffer tank is provided with a first inlet, a second inlet and a third inlet, and the bottom is provided with a bottom outlet; wherein the second inlet is connected to the cone of the first cyclone separator The third inlet is connected to the cylindrical top outlet of the second cyclone, the bottom outlet is connected to the middle inlet of the first cyclone, the second cyclone The middle inlet of the flow separator is connected with the drainage pipe respectively. It is beneficial to describe the specific structure and connection of the buffer tank.

In some embodiments, a pressure control valve and a pressure sensor are arranged on the pipeline connecting the second inlet and the outlet of the conical bottom of the first cyclone, and the third inlet is connected to the second cyclone. A pressure control valve and a pressure sensor are arranged on the pipeline connected to the columnar top outlet of the flow separator. It is beneficial to describe some of the settings associated with the top of the surge tank and each cyclone.

In some embodiments, a first valve, a pump, a pressure sensor and a flow sensor are provided on the pipeline connecting the bottom outlet and the middle inlet of the first cyclone, and the bottom outlet is connected to the second cyclone. A second valve, a pump, a pressure sensor and a flow sensor are arranged on the pipeline connected to the middle inlet of the cyclone separator. It is beneficial to describe some of the settings associated with the bottom of the surge tank and each cyclone.

In some embodiments, a pressure sensor and a flow sensor are provided on both the first waste discharge pipe and the second waste discharge pipe, and a third valve is provided on the liquid discharge pipe. It is beneficial to describe the relevant settings on each waste pipe.

According to one aspect of the present invention, there is provided a chromatography system using the above-mentioned circulating centrifugal separation system for impurities, which comprises a chromatography column, a main liquid inlet pipe and a main liquid discharge pipe, and the inside of the chromatography column has an upper sieve mesh and lower screen, and the bottom of the chromatography column has a bottom return port, a bottom mobile phase port, and the top has a plug mobile phase port, wherein the bottom return port is connected to the top of the buffer tank through the first pipeline The bottom mobile phase port is connected with the outlet end of the liquid inlet main pipe, and the plug mobile phase port is connected with the inlet of the liquid discharge main pipe.

In some embodiments, the main liquid inlet pipe and the main liquid discharge pipe are further communicated through a seventh valve, and the main liquid inlet pipe is also connected with the liquid discharge pipe. The advantage is that, by connecting the main liquid inlet pipe and the main liquid discharge pipe, the liquid can be discharged directly without going through the chromatography system.

In some embodiments, the inlet ends of the main liquid inlet pipe are respectively connected to the buffer outlet, the outlet of the liquid to be separated, the eluent outlet and the cleaning liquid outlet, and the main liquid inlet pipe is provided with an indicating pump, a transmitter, Pressure transmitter, conductivity transmitter and fifth valve. It is beneficial to describe some connections and settings related to the mains of the liquid feed.

In some embodiments, the outlet ends of the main liquid drainage pipeline are respectively connected to the product tank and the waste drainage pipeline, and the main liquid drainage pipeline is provided with a sixth valve, a pH sensor, a conductivity transmitter, and a flow indicating transmission device and UV sensor. It is beneficial to describe some connections and settings related to the drain mains.

In some embodiments, a fourth valve and a pressure transmitter are disposed on the first pipeline, and the first pipeline communicates with the main drainage pipeline through an eighth valve. It is beneficial to further describe some specific settings of the chromatography system.

Description of drawings

1 is a schematic structural diagram of a cyclic centrifugal separation impurity system according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a chromatography system using the circulating centrifugal separation system of impurities shown in FIG. 1;

FIG. 3 is a schematic diagram of part of the structure of the chromatography system shown in FIG. 1 .

In the figure: buffer tank 1, first inlet 2, second inlet 3, third inlet 4, bottom outlet 5, first cyclone separator 6, first valve 7, first waste pipe 8, second cyclone Separator 9, second valve 10, second waste pipe 11, drain pipe 12, third valve 13, chromatography column 20, bottom return port 21, bottom mobile phase port 22, plunger mobile phase port 23, first The fifth valve 24 , the sixth valve 25 , the main liquid inlet pipe 26 , the main liquid discharge pipe 27 , the seventh valve 28 , the product tank 29 , the waste pipe 30 , the eighth valve 31 , the fourth valve 32 , and the first pipe 33 .

Detailed ways

The present invention will be described in further detail below in conjunction with the accompanying drawings.

FIG. 1 schematically shows the structure of a circulating centrifugal separation system for impurities according to an embodiment of the present invention. As shown in FIG. 1 , the system for separating impurities mainly includes a buffer tank 1 , a first cyclone separator 6 , a second cyclone separator 9 , a first waste discharge pipeline 8 and a second waste discharge pipeline 11 . Wherein, the top of the buffer tank 1 is provided with a first inlet 2, a second inlet 3 and a third inlet 4, the bottom is provided with a bottom outlet 5, and the first cyclone 6 and the second cyclone 9 both have columns Shaped top, middle inlet and tapered bottom outlet. The above-mentioned structures are connected to each other in a specific sequence of pipeline installation to form the impurity separation system.

The second inlet 3 is connected with the conical bottom outlet of the first cyclone 6, and a pressure control valve and a pressure sensor (set the measured pressure as Pu) are arranged on the connected pipeline, and the bottom outlet 5 is connected to the first cyclone. The middle inlets of the cyclone separators 6 are connected, and the connected pipelines are provided with a first valve 7, a pump, a pressure sensor (set the measured pressure as Pf) and a flow sensor (set the measured flow as Ff). Wherein, the cylindrical top outlet of the first cyclone 6 is connected with the first waste discharge pipeline 8, and the first waste discharge pipeline 8 is provided with a pressure sensor (set the measured pressure as Po) and a flow sensor ( Assuming that the measured flow rate is Fo), the pressure difference in the line is DP=Pu-Po, and the overflow ratio is Fo/Ff.

The third inlet 4 is connected to the cylindrical top outlet of the second cyclone 9, and a pressure control valve and a pressure sensor (set the measured pressure to be Po) are arranged on the connected pipeline, and the bottom outlet 5 is connected to the first outlet. The middle inlets of the two cyclone separators 9 are connected to each other, and a second valve 10, a pump, a pressure sensor (set the measured pressure as Pf) and a flow sensor (set as the measured flow rate as Ff) are arranged on the connected pipeline. . Wherein, the conical bottom outlet of the second cyclone separator 9 is connected with the second waste discharge pipeline 11, and the second waste discharge pipeline 11 is provided with a pressure sensor (set the measured pressure as Pu) and a flow sensor (set The measured flow rate is Fu), the pressure difference in the line is DP=Pu-Po, and the overflow ratio is (Ff-Fu)/Ff.

In addition, the bottom outlet 5 of the buffer tank 1 is also connected with a drain pipe 12 , and a third valve 13 is arranged on the drain pipe 12 .

When using the circulating centrifugal separation system for impurities, open the pressure control valve, the first valve 7 and the second valve 10, and close the third valve 13, the liquid to be separated enters the buffer tank 1 through the first inlet 2, and then The liquid enters the first cyclone separator 6 and the second cyclone separator 9 from the bottom outlet 5 of the buffer tank 1 respectively for separation.

Among them, in the first cyclone separator 6, the oily substances with lower density in the liquid are discharged through its cylindrical top outlet, and the clear liquid and slag with higher density are discharged through its conical bottom, and then re-enter the buffer tank 1; while in the second cyclone 9, the oil and clear liquid with less density in the liquid are discharged through its cylindrical top outlet, and then re-enter the buffer tank 1, while the clear liquid and slag with high density are discharged It is then discharged through its conical bottom. In this way, the liquid can be circulated and separated in the buffer tank 1 until only the required clear liquid is left in the tank, and the rest of the oil and slag are discharged from the tank. After the separation of impurities meets the requirements, the third Valve 13 performs discharge delivery.

In addition, the clear liquid and slag in the two cyclones are discharged through the first waste discharge pipeline 8 and the second waste discharge pipeline 11 respectively.

FIG. 2 shows the structure of a chromatography system to which the circulating centrifugal separation system of FIG. 1 is applied, and FIG. 3 shows a part of the structure of the chromatography system of FIG. 2 . As shown in FIGS. 2-3 , the system mainly includes the continuous centrifugal separation impurity system in FIG. 1 , a chromatography column 20 , a main liquid inlet pipe 26 and a main liquid discharge pipe 27 . The interior of the chromatography column 20 has an upper sieve and a lower sieve, the bottom of the chromatography column 20 has a bottom return port 21 , a bottom mobile phase port 22 , and the top has a plug mobile phase port 23 .

The bottom return port 21 is connected to the first inlet 2 at the top of the buffer tank 1 through a first pipe 33 , and a fourth valve 32 and a pressure transmitter are arranged on the first pipe 33 .

The bottom mobile phase port 22 is connected to the outlet end of the main liquid inlet pipe 26, and the inlet end of the main liquid inlet pipe 26 is connected to a plurality of liquid pipe outlets, including the buffer liquid outlet, the liquid to be separated outlet, the eluent outlet and the cleaning liquid outlet. Liquid outlet, etc. Wherein, the main liquid inlet pipeline 26 is provided with an indicating pump, a transmitter, a pressure transmitter, a conductivity transmitter and a fifth valve 24 . In addition, the main liquid inlet pipe 26 is also connected with the liquid discharge pipe 12 .

The plug mobile phase port 23 is connected to the inlet of the main drain pipe 27, and the outlet ends of the main drain pipe 27 are connected to the product tank 29 and the waste drain pipe 30, respectively. Among them, a sixth valve 25 , a pH sensor, a conductivity transmitter, a flow indicating transmitter and an ultraviolet sensor are arranged on the main liquid discharge pipeline 27 .

In addition, the main liquid inlet pipe 26 and the main liquid discharge pipe 27 are also communicated with each other through the seventh valve 28 , and the first pipe 33 is also communicated with the main liquid discharge pipe 27 through the eighth valve 31 .

When using the chromatography system, the liquid to be separated enters the chromatography column 20 through the liquid inlet main pipe 26, wherein a part of the liquid enters the main body through the lower screen, and then passes through the upper screen and then flows out of the plunger. The mobile phase port 21 flows out, and then enters the waste discharge pipe 19 through the main liquid outlet pipe 27 .

And a part of the liquid does not enter the chromatography column 20, but enters the buffer tank 1 through the pipeline after passing through the reflux port 21 at the bottom of the chromatography column 20 for separation, and the separated liquid with higher density The clear liquid is discharged through the discharge pipe 12 and enters the main liquid inlet pipe 26 . At this time, the fourth valve 32 is closed to allow the clear liquid to pass through the chromatography column 20 for purification.

When the liquid does not need to pass through the chromatography column 20, only the seventh valve 28 needs to be opened. At this time, the liquid will enter the product tank 29 according to the settings after passing through the main liquid inlet pipe 26, the seventh valve 28 and the main liquid discharge pipe 27. Or the waste pipe 30 .

When elution is required, the eighth valve 31 , the fifth valve 24 and the sixth valve 25 are opened, the eluent enters the chromatography column 20 through the liquid inlet main pipeline 26 , and then passes through the bottom return port of the chromatography column 20 respectively. 21 and the plug mobile phase port 23 of the chromatography column 20 enter into the main drainage pipe 27 and finally enter the product tank 29 .

The foregoing are merely some of the embodiments of the present invention. For those of ordinary skill in the art, without departing from the inventive concept of the present invention, several modifications and improvements can be made, which all belong to the protection scope of the present invention.

Claims (10)

1. A circulating centrifugal separation impurity system is characterized in that: comprise a buffer tank (1), and the top of the buffer tank (1) is respectively connected to the conical bottom outlet of a first cyclone (6) and a The cylindrical top outlet of the second cyclone (9), the bottom of the buffer tank (1) and the middle inlet of the first cyclone (6), the second cyclone (9) ) and a drain pipe (12) are respectively connected, wherein the cylindrical top outlet of the first cyclone (6) is connected to a first waste pipe (8), the second cyclone The conical bottom outlet of the flow separator (9) is connected to a second waste discharge pipe (11). 2. A system for circulating centrifugal separation of impurities according to claim 1, characterized in that: the top of the buffer tank (1) is provided with a first inlet (2), a second inlet (3) and a third inlet ( 4), the bottom is provided with a bottom outlet (5); wherein, the second inlet (3) is connected with the conical bottom outlet of the first cyclone (6), and the third inlet (4) It is connected with the cylindrical top outlet of the second cyclone (9), and the bottom outlet (5) is separated from the middle inlet of the first cyclone (6) and the second cyclone. The inlet of the middle part of the device (9) is connected with the drainage pipe (12) respectively. 3. A circulating centrifugal separation impurity system according to claim 2, characterized in that: the pipeline connecting the second inlet (3) and the conical bottom outlet of the first cyclone (6) A pressure control valve and a pressure sensor are arranged thereon, and a pressure control valve and a pressure sensor are arranged on the pipeline connecting the third inlet (4) with the cylindrical top outlet of the second cyclone (9). 4. A circulating centrifugal separation impurity system according to claim 2, characterized in that: the pipe connecting the bottom outlet (5) and the middle inlet of the first cyclone (6) is provided with a A first valve (7), a pump, a pressure sensor and a flow sensor, a second valve (10) is provided on the pipeline connecting the bottom outlet (5) with the middle inlet of the second cyclone (9) , pumps, pressure sensors and flow sensors. 5 . The system according to claim 1 , wherein the first waste discharge pipe ( 8 ) and the second waste discharge pipe ( 11 ) are both provided with a pressure sensor and a flow rate. 6 . In the sensor, a third valve (13) is arranged on the liquid discharge pipeline (12). 6. A chromatography system using the circulating centrifugal separation impurity system according to any one of claims 1-5 is characterized in that: comprising a chromatography column (20), a liquid inlet main pipe (26) and a drain The main pipeline (27), the interior of the chromatography column (20) has an upper screen and a lower screen, and the bottom of the chromatography column (20) is provided with a bottom reflux port (21) and a bottom mobile phase port (22). ), the top has a plunger mobile phase port (23), wherein the bottom return port (21) is connected to the top of the buffer tank (1) through a first pipeline (33), and the bottom mobile phase port ( 22) is connected to the outlet end of the main liquid inlet pipe (26), and the plug mobile phase port (23) is connected to the inlet of the main liquid discharge pipe (27). 7. A chromatography system using a circulating centrifugal separation system for impurities according to claim 6, characterized in that: the main liquid inlet pipe (26) and the main liquid discharge pipe (27) also pass through the seventh The valve (28) is communicated with each other, and the main liquid inlet pipe (26) is also connected with the liquid discharge pipe (12). 8. A chromatography system using a circulating centrifugal separation impurity system according to claim 6, characterized in that: the inlet end of the main liquid inlet pipe (26) is respectively connected to the buffer outlet, the outlet of the liquid to be separated, The eluent outlet and the cleaning liquid outlet are provided with an indicating pump, a transmitter, a pressure transmitter, a conductivity transmitter and a fifth valve (24) on the main liquid inlet pipe (26). 9. A chromatography system using a circulating centrifugal separation impurity system according to claim 6, characterized in that: the outlet ends of the main drainage pipes (27) are respectively connected to the product tank (29) and the waste drainage pipes (30), and a sixth valve (25), a pH sensor, a conductivity transmitter, a flow indicating transmitter and an ultraviolet sensor are arranged on the main draining pipeline (27). 10. A chromatography system using a circulating centrifugal separation impurity system according to claim 6, characterized in that: the first pipeline (33) is provided with a fourth valve (32) and a pressure transmitter, The first pipe (33) communicates with the main drain pipe (27) through an eighth valve (31).

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