CN115282101A - Pet bath lotion capable of reducing stress response of cats and preparation method of pet bath lotion - Google Patents
Pet bath lotion capable of reducing stress response of cats and preparation method of pet bath lotion Download PDFInfo
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- CN115282101A CN115282101A CN202210767937.6A CN202210767937A CN115282101A CN 115282101 A CN115282101 A CN 115282101A CN 202210767937 A CN202210767937 A CN 202210767937A CN 115282101 A CN115282101 A CN 115282101A
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A—HUMAN NECESSITIES
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- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A—HUMAN NECESSITIES
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- Mycology (AREA)
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- Pain & Pain Management (AREA)
- Toxicology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a pet shower gel for reducing stress response of cats and a preparation method thereof, wherein the pet shower gel for reducing stress response of cats comprises a conditioner, a humectant, an emulsifier, a thickener, a pH regulator and water; the conditioner comprises Actinidia polygama extract, nepeta cataria extract, verbena officinalis extract, marigold extract and lemon grass extract. The five conditioner components supplement each other, have synergistic effect, and have remarkable effects in relieving the mood of the cat, reducing the stress response of the cat, inhibiting bacteria, diminishing inflammation and caring hair.
Description
Technical Field
The invention belongs to the technical field of daily chemical products for pets, relates to pet shower gel for reducing cat stress reaction and a preparation method thereof, and particularly relates to pet shower gel with the effects of reducing cat stress reaction, inhibiting bacteria, diminishing inflammation and caring hair and a preparation method thereof.
Background
The stress response is caused by external stimulation, which causes a series of changes in the animal body, such as increase of secretion of various hormones, increase of blood pressure, increase of blood sugar and the like. Stress may occur in every organism, but because cats are inherently very small and more sensitive to external stimuli, they are more likely to produce stress.
The cat is a very small and sensitive animal, is very sensitive to strange environment, the smell sense of the cat is very sensitive, and the new environment and smell change with the original environment, so that the cat is more uneasy. The strong stress response of the cat can be caused by the cat not frightening, going out and bathing once.
When the cat is stressed, the physiology changes as follows: hair frying, mydriasis, tachypnea, tachycardia, running water, slow growth of kittens and the like. Change of behavior: stiff and immobile, flee, hide, make a hoarse sound, meow and meow, over-grooming the hair, etc. The most common people are frightened and cannot lie under the bed and the sofa independently for a long time. The serious points are vomiting, diarrhea and defecation anywhere. The more serious, such as blocked urethra, is frequent toilet use but constipation and urine do not come out. And worse yet, it appears to have nothing in several consecutive days and howl. Most serious are generalized cramps, rapid abdominal undulations, eyeball bulging, and death at the time of or shortly after the occurrence of stress.
There are many sequelae to stress, which remain after the symptoms are therapeutically alleviated and threaten the health of the cat. For example, a prolonged blockage of the urethra can cause severe renal failure after the kidney, which is a serious life-threatening condition. If people do not eat the cat food for a long time, a large amount of fat cells can be decomposed by the body, the fat cells are deposited in the liver to cause the fatty liver, and the fatty liver can adversely affect the appetite of the cat, so that the cat does not eat the food and vicious circle. We need to be particularly cautious in the face of cat stress.
The pet hair is long and easily gets dirty, and needs to be cleaned regularly. Some pets mainly feel that cats are dirty, so cats are often helped to take a bath, but many pets only pay attention to the basic cleaning function, hair and skin care ingredients are lacked, and frequent bath of cats with the shampoo can easily cause the grease protective layer on the skin of the cat to be damaged, so that the cats have the phenomena of hair loss, tear marks and the like, and even suffer from skin diseases. In addition, the hair and the skin of the pet cat have a large amount of bacteria and fungi, so the pet cat is easy to suffer from skin diseases, and particularly, the wet environment is very easy to be infected with fungi tinea pedis. At present, the raising of pets is very common, and care products for the pets, such as bath cream in the aspect of cleaning, pay more and more attention to the comprehensiveness of the quality and the efficacy of the products. The specific cat-stress-resistant functional pet shower gel disclosed in the prior art is still fresh and few.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide the pet shower gel for reducing the stress response of cats and the preparation method thereof, and particularly provides the pet shower gel with the effects of reducing the stress response of cats, inhibiting bacteria, diminishing inflammation and caring hair and the preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a pet bath lotion for reducing cat stress, which comprises a conditioner, a humectant, an emulsifier, a thickener, a pH regulator and water;
the conditioner comprises Actinidia polygama extract, nepeta cataria extract, verbena officinalis extract, marigold extract and lemon grass extract.
Five conditioner components including a Actinidia polygama extract, a catnip extract, a verbena extract, a calendula extract and a lemongrass extract are added into the pet shower gel related by the invention at the same time, wherein the Actinidia polygama extract and the catnip extract both contain nepetalactone, so that the emotion of cats can be relieved, the stress response of cats can be reduced, and the pet shower gel also has good effects of hair care, antibiosis and itching relief, mosquito repelling, cat moss removal and the like; the verbena extract, the calendula extract and the lemongrass extract are extracted from favorite plants of the cat, have unique aromatic substances, enable the cat to feel comfortable and pleasant, relieve the mood of the cat, reduce the stress response of the cat, have good anti-inflammatory and antibacterial effects, have certain auxiliary effect on cat moss, and can nourish the skin of the cat. The five components supplement each other, have synergistic effect, and have remarkable effects in relieving the mood of the cat, reducing the stress response of the cat, inhibiting bacteria, diminishing inflammation and caring hair.
Preferably, the pet bath cream for reducing the stress response of the cats comprises, by weight, 5-30 parts of a conditioner, 0.1-10 parts of a humectant, 25-60 parts of an emulsifier, 5-30 parts of a thickener, 0.01-0.2 part of a pH regulator and water;
the conditioner comprises, by weight, 1-10 parts of Actinidia polygama extract, 1-10 parts of Nepeta catnip extract, 1-10 parts of Verbena officinalis extract, 1-10 parts of calendula officinalis extract and 1-10 parts of lemon grass extract.
When the five conditioner components of the polygonum capitatum extract, the catnip extract, the verbena extract, the calendula extract and the lemongrass extract are matched with other conventional components in the shower gel according to the specific mass ratio relationship, the effects of the conditioner on easing the emotion of the cat, reducing the stress response of the cat, inhibiting bacteria and diminishing inflammation and caring hair are more obvious.
The conditioner can be 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
The humectant can be 0.1 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like by weight.
The emulsifier can be 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts and the like.
The weight parts of the thickening agent can be 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts and the like.
The weight parts of the pH regulator can be 0.01 part, 0.03 part, 0.05 part, 0.08 part, 0.1 part, 0.12 part, 0.15 part, 0.17 part, 0.2 part and the like.
The parts by weight of the Actinidia polygama extract, the Nepeta cataria extract, the Verbena officinalis extract, the calendula officinalis extract and the lemon grass extract are respectively and independently selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the conditioner further comprises thyme oil and rosemary oil.
The invention also creatively discovers that the further addition of thyme oil and rosemary oil in the shower gel can promote the effects of the shower gel in aspects of relaxing the mood of the cat, reducing the stress response of the cat, inhibiting bacteria, diminishing inflammation and caring hair. And the thyme oil and the rosemary oil have certain synergistic effect.
Preferably, the conditioner also comprises 0.1-1 part of thyme oil and 0.1-1 part of rosemary oil in parts by weight.
The weight parts of the thyme oil and the rosemary oil are respectively and independently selected from 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like.
Other specific values within the above ranges can be selected, and are not described in detail herein.
Further preferably, the conditioning agent further comprises gamma-aminobutyric acid.
The invention also creatively discovers that the gamma-aminobutyric acid is further added into the shower gel to promote the effects of the shower gel on the aspects of relaxing the mood of the cat and reducing the stress response of the cat.
Preferably, the conditioner further comprises 0.01-1 parts by weight of gamma-aminobutyric acid, such as 0.01 parts, 0.05 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part and the like. Other specific point values in the numerical range can be selected, and are not described in detail herein.
Preferably, the moisturizer comprises any one of phytosterols, hydrolyzed wheat protein, hydrolyzed fibroin or a combination of at least two thereof.
Preferably, the emulsifier comprises any one of sodium cocamidopropionate, cocamidopropyl betaine, cocamidomethyl MEA, sodium cocoyl isethionate, vegetable alkyl glycoside or a combination of at least two thereof. Preferred are combinations of sodium cocamidopropionate, cocamidopropyl betaine, cocamidomethyl MEA, sodium cocoyl isethionate and vegetable alkyl glycosides.
The pet shower gel system related by the invention takes sodium cocoamidopropionate and cocoamidopropyl betaine as main surfactants, and takes cocoamidomethyl MEA, sodium cocoyl isethionate and plant alkyl glycoside as auxiliary surfactants, and the five surfactants are matched, so that the pet shower gel system not only has good effects of foaming, stabilizing and cleaning pet fur, but also has low degreasing power, low irritation and mild and no irritation, and can make a cat feel comfortable and pleasant, alleviate the mood of the cat and reduce the stress reaction of the cat; the more synergistic conditioner promotes the moisturizing and antibacterial effects of the shower gel.
Preferably, the thickener comprises any one of lauramidopropyl hydroxysultaine, cationic guar gum, sodium chloride, 16-18 alcohol, lauryl alcohol, myristyl alcohol, linoleic acid, linolenic acid, or a combination of at least two thereof.
Preferably, the pH adjusting agent comprises citric acid and/or sodium citrate.
Preferably, the pet bath cream for reducing the stress response of cats further comprises any one or the combination of two of a chelating agent and a preservative.
Preferably, the chelating agent comprises disodium edetate.
Preferably, the pet bath cream for reducing the stress response of cats further comprises any one or a combination of at least two of 0.01-0.5 part of a chelating agent and 0.1-1 part of a preservative in parts by weight.
The chelating agent may be present in an amount of 0.01 parts, 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, 0.5 parts, etc.
The preservative can be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like in parts by weight.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
In a second aspect, the invention provides a preparation method of the pet shower gel for reducing cat stress response, which comprises the following steps:
(1) Mixing an emulsifier with water to obtain a first mixed solution;
(2) And mixing the first mixed solution with a conditioner, a thickening agent, a humectant and a pH regulator to obtain the water-based emulsion.
The preparation process of the pet shower gel provided by the invention is simple, convenient to operate, low in cost, suitable for industrial production and high in practicability.
Preferably, the water of step (1) is mixed with the chelating agent before being mixed with the emulsifier.
Preferably, the step (2) is further mixed with a preservative after mixing.
Preferably, the temperature of the mixing in step (1) is 80-85 deg.C, such as 80 deg.C, 81 deg.C, 82 deg.C, 83 deg.C, 84 deg.C, 85 deg.C, etc.; mixing for 5-20min, such as 5min, 8min, 10min, 12min, 15min, 18min, 20min, etc.
Preferably, the temperature of the mixing in step (2) is 40-60 deg.C, such as 40 deg.C, 42 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 58 deg.C, 60 deg.C, etc.; mixing for 5-20min, such as 5min, 8min, 10min, 12min, 15min, 18min, 20min, etc.
Compared with the prior art, the invention has the following beneficial effects:
five conditioner components including a Actinidia polygama extract, a catnip extract, a verbena extract, a calendula extract and a lemongrass extract are added into the pet shower gel related by the invention at the same time, wherein the Actinidia polygama extract and the catnip extract both contain nepetalactone, so that the emotion of cats can be relieved, the stress response of cats can be reduced, and the pet shower gel also has good effects of hair care, antibiosis and itching relief, mosquito repelling, cat moss removal and the like; the verbena extract, the calendula extract and the lemongrass extract are extracted from favorite plants of the cat, have unique aromatic substances, enable the cat to feel comfortable and pleasant, relieve the mood of the cat, reduce the stress response of the cat, have good anti-inflammatory and antibacterial effects, have certain auxiliary effect on cat moss, and can nourish the skin of the cat. The five components supplement each other, have synergistic effect, and have remarkable effects in relieving the mood of the cat, reducing the stress response of the cat, inhibiting bacteria, diminishing inflammation and caring hair.
The invention also creatively discovers that the further addition of thyme oil and rosemary oil in the shower gel can promote the effects of the shower gel in aspects of relaxing the mood of the cat, reducing the stress response of the cat, inhibiting bacteria, diminishing inflammation and caring hair. And the thyme oil and the rosemary oil have certain synergistic effect. Thyme oil and rosemary oil are used for replacing the chemical flavoring agent, so that the toxic and side effects of the chemical flavoring agent on the cats can be avoided.
The invention also creatively discovers that the effect of the shower gel on relieving the mood of the cat and reducing the stress response of the cat can be promoted by further adding the gamma-aminobutyric acid into the shower gel.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The following examples and comparative examples relate to the following raw material source information as shown in the following table:
example 1
The embodiment provides pet shower gel for reducing stress response of cats, which comprises the following components in percentage by weight:
conditioners (polygonum minus 5%, catnip extract 3%, verbena extract 2%, calendula extract 6%, lemongrass extract 8%, thyme oil 0.3%, rosemary oil 0.2%, γ -aminobutyric acid 0.1%), humectants (phytosterols 1%), emulsifiers (sodium cocoamidopropionate 9%, cocamidopropyl betaine 6%, cocamidomethyl MEA 2%, sodium cocoyl isethionate 1%, vegetable alkyl glycosides 2%), thickeners (lauramidopropyl hydroxysultaine 10%, cationic guar gum 0.5%), pH regulators (citric acid, regulating system to pH = 6.5), chelating agents (EDTA 2Na 0.1%), water make up to 100%.
The preparation method comprises the following steps:
(1) Mixing water with EDTA 2Na, and then mixing with an emulsifier at 80 ℃ for 15min to obtain a first mixed solution;
(2) Mixing the first mixed solution with conditioner, thickener, humectant and pH regulator, and stirring at 50 deg.C for 15min.
Example 2
The embodiment provides pet shower gel for reducing stress response of cats, which comprises the following components in percentage by weight:
conditioners (polygonum minus 7%, catnip extract 1%, verbena extract 6%, calendula extract 8%, lemongrass extract 2%, thyme oil 0.1%, rosemary oil 0.4%, γ -aminobutyric acid 0.1%), humectants (phytosterols 1%), emulsifiers (sodium cocoamidopropionate 12%, cocamidopropyl betaine 3%, cocamidomethyl MEA 4%, sodium cocoyl isethionate 0.5%, vegetable alkyl glycosides 0.5%), thickeners (lauramidopropyl hydroxysultaine 10%, cationic guar gum 0.5%), pH regulators (citric acid, regulating system to pH = 6.5), chelating agents (EDTA 2Na 0.1%), water make up to 100%.
The preparation method comprises the following steps:
(1) Mixing water with EDTA 2Na, and then mixing with an emulsifier at 70 ℃ for 20min to obtain a first mixed solution;
(2) Mixing the first mixed solution with conditioner, thickener, humectant and pH regulator, and stirring at 40 deg.C for 20min.
Comparative example 1
The comparative example provides a pet bath lotion for reducing stress response of cats, and the formulation of the pet bath lotion is different from that of example 1 only in that the polygonum capitatum extract ingredient is absent, the mass of the polygonum capitatum extract ingredient is proportionally distributed to the mass of the catnip extract, the verbena extract, the calendula extract and the lemongrass extract, and other components and the content of the other components are kept unchanged. The preparation process is as in example 1.
Comparative example 2
The comparative example provides a pet bath lotion for reducing cat stress, and the formula of the pet bath lotion is different from that of example 1 only in that the catmint extract ingredient is absent, the catmint extract ingredient is proportionally distributed on the mass of the polygonum multiflorum extract, the verbena extract, the calendula extract and the lemongrass extract, and other components and the content of the components are kept unchanged. The preparation process is as in example 1.
Comparative example 3
The comparative example provides a pet bath lotion for reducing stress response of cats, the formulation of which is different from that of example 1 only in that the ingredients of the verbena extract are lacked, the mass of the verbena extract is proportionally distributed on the mass of the polygonum multiflorum extract, the catnip extract, the calendula extract and the lemongrass extract, and other components and the content thereof are kept unchanged. The preparation process is as in example 1.
Comparative example 4
The comparative example provides a pet bath lotion for reducing cat stress, and the formula of the pet bath lotion is different from that of example 1 only in that calendula officinalis extract ingredients are lacked, the mass of the calendula officinalis extract ingredients is proportionally distributed on the mass of Actinidia polygama extract, nepeta cataria extract, verbena extract and lemon grass extract, and other components and the content of the components are kept unchanged. The preparation process is as in example 1.
Comparative example 5
The comparative example provides a pet bath lotion for reducing cat stress, the formula of which is different from that of example 1 only in that the lemon grass extract ingredient is lacked, the mass of the lemon grass extract ingredient is proportionally distributed on the mass of the Actinidia polygama extract, the catnip extract, the verbena extract and the calendula extract, and other components and the content thereof are kept unchanged. The preparation process is as in example 1.
Comparative example 6
The comparative example provides a pet bath lotion for reducing the stress response of cats, and the formulation of the pet bath lotion is only different from that of example 1 in that 5% of Actinidia polygama extract, 3% of Nepeta cataria extract, 2% of Verbena officinalis extract, 6% of calendula officinalis extract and 8% of lemon grass extract are replaced by 24% of Actinidia polygama extract, and other components and the content thereof are kept unchanged. The preparation method is referred to example 1.
Comparative example 7
The comparative example provides a pet bath lotion for reducing the stress response of cats, and the formulation of the pet bath lotion is only different from that of example 1 in that 5% of Actinidia polygama extract, 3% of Nepeta cataria extract, 2% of Verbena officinalis extract, 6% of calendula officinalis extract and 8% of lemon grass extract are replaced by 24% of Nepeta cataria extract, and other components and the content thereof are kept unchanged. The preparation process is as in example 1.
Comparative example 8
The comparative example provides a pet bath lotion for reducing the stress response of cats, and the formulation of the pet bath lotion is only different from that of example 1 in that 5% of polygonum praeparatum extract, 3% of catnip extract, 2% of verbena extract, 6% of calendula extract and 8% of lemon grass extract are replaced by 24% of verbena extract, and other components and the content thereof are kept unchanged. The preparation process is as in example 1.
Comparative example 9
The comparative example provides a pet bath lotion for reducing cat stress response, and the formula of the pet bath lotion is different from that of example 1 only in that 5% of polygonum minus extract, 3% of catnip extract, 2% of verbena extract, 6% of calendula extract and 8% of lemon grass extract are replaced by 24% of calendula extract, and other components and the content thereof are kept unchanged. The preparation process is as in example 1.
Comparative example 10
The comparative example provides a pet bath lotion for reducing the stress response of cats, and the formulation of the pet bath lotion is only different from that of example 1 in that 5% of Actinidia polygama extract, 3% of Nepeta cataria extract, 2% of Verbena officinalis extract, 6% of calendula officinalis extract and 8% of lemon grass extract are replaced by 24% of lemon grass extract, and other components and the content thereof are kept unchanged. The preparation process is as in example 1.
Example 3
This example provides a pet shower gel for reducing stress response of cats, which is prepared according to the method of example 1, wherein the formula of the pet shower gel is only different from that of example 1 in that 0.3% of thyme oil and 0.2% of rosemary oil are replaced by 0.5% of thyme oil, and other components and the content thereof are kept unchanged. The preparation process is as in example 1.
Example 4
This example provides a pet bath lotion for reducing stress response of cats, which is prepared according to the following formula that only 0.3% of thyme oil and 0.2% of rosemary oil are replaced by 0.5% of rosemary oil, and other components and the content thereof are kept unchanged from example 1. The preparation process is as in example 1.
Example 5
This example provides a pet shower gel for reducing stress in cats, which is formulated in the absence of the gamma aminobutyric acid component and the remaining components and their amounts are maintained as described in example 1. The preparation process is as in example 1.
Example 6
This example provides a pet shower gel for reducing stress in cats, which is formulated in the absence of thyme oil, rosemary oil, and gamma aminobutyric acid, and the other components and their amounts remain the same as in example 1. The preparation process is as in example 1.
Test example 1
The safety evaluation was carried out on the shower gel products prepared in examples 1 to 6:
(1) Erythrocyte hemolysis assay
Preparation of erythrocyte suspension: selecting healthy rabbit, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use. Each set of samples was selected for dilution to different concentrations with PBS solution, with 5 concentration gradients set for each sample. Taking 10mL of a diluent of a sample to be tested, adding 200 μ L of the erythrocyte suspension (controlling the final concentration of the sample to be 5, 10, 20, 50 and 100mg/mL respectively), taking distilled water as a total blood-dissolving control, taking a PBS solution as a negative control, gently mixing, incubating for 30min at 37 ℃, centrifuging for 10min at the rotating speed of 2000r/min, taking a supernatant, testing the absorbance (A560) of the supernatant at 560nm by using a spectrophotometer, and calculating the hemolysis rate according to the following formula;
hemolysis rate = [ (experimental group) A560 Negative control group A560 ) /(Whole blood control group) A560 Negative control group A560 )]×100％
A standard curve of the hemolysis rate versus the concentration of the sample was plotted, and the concentration of the sample at which 50% of erythrocytes were hemolyzed (HD 50) was calculated.
(2) Protein denaturation assay
Diluting each group of samples to 10g/L by PBS solution, taking 10mL of diluent of a sample to be detected, adding 200 μ L of the erythrocyte suspension, taking distilled water as a blank control, taking 1mg/mL of Sodium Dodecyl Sulfate (SDS) solution as a positive control, gently mixing, incubating for 30min at 37 ℃, centrifuging for 10min at the rotating speed of 2000r/min, taking supernatant, respectively testing the absorbances A540 and A575 at 540nm and 575nm by a spectrophotometer, and calculating the protein Denaturation Index (DI) according to the following formula;
DI＝[(R 1 -R 2 )/(R 1 -R 3 )]×100％
wherein R is 1 = blank control group a 575 Blank control group A 540 ，R 2 = Experimental group A 575 Experimental group A 540 ，R 3 = positive control group a 575 Positive control group A 540 。
The samples to be tested were evaluated for their irritability according to the L/D value, which is HD50/DI, and the irritation grading criteria are shown in Table 1 below:
TABLE 1
| L/D | Grading |
| ＞100 | Has no irritation |
| 10＜L/D≤100 | Micro-stimulation property |
| 1＜L/D≤10 | Mild irritation |
| 0.1＜L/D≤1 | Moderate irritation |
The above test results are shown in table 2:
TABLE 2
As can be seen from the data in Table 2: the pet shower gel provided by the invention is very mild, and has no stimulation to the skin, hair and eyes of pets.
Test example 2
The pet bath cream products provided by examples 1-6 and comparative examples 1-10 were evaluated for bacteriostatic effect by the following method:
by referring to the test method (suspension quantification method) for testing the bacteriostatic effect of the bacteriostatic daily chemical product in QB/T2738-2012, 10g of the products prepared in examples 1-6 and comparative examples 1-10 were completely dissolved in 300mL of distilled water, and the action time with the microorganisms was 15min. Results of bacteriostatic rate are shown in table 3:
TABLE 3
From the data results of table 3, it can be seen that: the shower gel for pets has remarkable antibacterial effect, and five plant extracts of the polygonum capitatum extract, the catnip extract, the verbena extract, the calendula extract and the lemon grass extract in the formula have certain synergistic effect on the exertion of the antibacterial effect. And the addition of thyme oil and rosemary oil further promotes its antibacterial efficacy.
Test example 3
And (3) testing the anti-stress performance:
test samples: examples 1, 3-6 and comparative examples 1-10;
the test method comprises the following steps: the same variety of 2-5 month-old weaned cats 80 were selected and kept in the same environment, except that different groups of shower gel products were used, 5 per group. Before bathing, pouring the pet bath cream for each test into a small cup, placing the small cup in a bathroom for 10min (a blank group uses conventional bath cream), placing the cat into the bathroom, closing the door, allowing the cat to freely move for 10min, observing behavior and emotion changes of the cat, touching and observing the cat after 10min, and starting bathing after reaction of the cat; the cats were soaked slowly with warm water, the amount of body wash required per cat was calculated at a dose of 1.5mL/Kg body weight, the body wash was applied to the body of the cat (avoiding eyes, nose and mouth), shampooed and massaged to ensure that the body wash was in contact with the skin and hair for 10min, and then rinsed clean with water, which could be worn by a collar to ensure that cats did not lick themselves or foam during bathing, 1 time per week. After 4 weeks, the kittens were given a bath in the same manner as described above, and each group of kittens was observed for the presence of the following stressful behaviors before and during the bath: the traditional Chinese medicine composition is prepared by the following steps of (1) curling or immobilizing the whole body, (2) trembling, (3) shortness of breath, (4) pupil enlargement, (5) saliva flowing or swallowing always, (6) meow, (7) hiding in corners, and (8) ear flattening. And (4) calculating the total score by analogy with 0 score of no stress behavior, 1 score of 1 behavior and 2 scores of 2 behaviors, and counting the average of each group.
The test results are shown in table 4:
TABLE 4
The data in table 4 show that the body wash for the pet, provided by the invention, has a remarkable effect of reducing the stress response of the cat, wherein five plant extracts, namely the Actinidia polygama extract, the Nepeta cataria extract, the verbena extract, the calendula extract and the lemongrass extract, have a certain synergistic effect on the effect of reducing the stress response of the cat. And the anti-stress effect of the thyme oil, the rosemary oil and the gamma-aminobutyric acid is further promoted by adding the thyme oil, the rosemary oil and the gamma-aminobutyric acid.
The applicant states that the invention is illustrated by the above examples to describe a pet bath lotion for reducing cat stress and a preparation method thereof, but the invention is not limited to the above examples, that is, the invention is not limited to the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
Claims (10)
1. The pet shower gel for reducing the stress response of cats is characterized by comprising a conditioner, a humectant, an emulsifier, a thickener, a pH regulator and water;
the conditioner comprises Actinidia polygama extract, nepeta cataria extract, verbena officinalis extract, marigold extract and lemon grass extract.
2. The pet bath cream for reducing the stress response of cats according to claim 1, wherein the pet bath cream for reducing the stress response of cats comprises, by weight, 5-30 parts of a conditioner, 0.1-10 parts of a humectant, 25-60 parts of an emulsifier, 5-30 parts of a thickener, 0.01-0.2 part of a pH regulator and water;
the conditioner comprises, by weight, 1-10 parts of Actinidia polygama extract, 1-10 parts of Nepeta catnip extract, 1-10 parts of Verbena officinalis extract, 1-10 parts of calendula officinalis extract and 1-10 parts of lemon grass extract.
3. The pet shower gel for reducing stress in cats according to claim 2, wherein the conditioner further comprises thyme oil and rosemary oil;
preferably, the conditioner also comprises 0.1-1 part of thyme oil and 0.1-1 part of rosemary oil in parts by weight.
4. The body wash for pet that reduces stress in cats according to claim 2, wherein the conditioning agent further comprises gamma-aminobutyric acid;
preferably, the conditioner also comprises 0.01-1 part of gamma-aminobutyric acid in parts by weight.
5. The pet body wash reducing cat stress according to any one of claims 1 to 4, wherein the moisturizer comprises any one of phytosterols, hydrolyzed wheat protein, hydrolyzed fibroin or a combination of at least two thereof;
preferably, the emulsifier comprises any one of sodium cocamidopropionate, cocamidopropyl betaine, cocamidomethyl MEA, sodium cocoyl isethionate, vegetable alkyl glycoside or a combination of at least two thereof.
6. The pet body wash reducing cat stress according to any one of claims 1 to 5, wherein the thickener comprises any one of lauramidopropyl hydroxysultaine, cationic guar, sodium chloride, 16-18 alcohol, lauryl alcohol, myristyl alcohol, linoleic acid, linolenic acid, or a combination of at least two thereof;
preferably, the pH adjusting agent comprises citric acid and/or sodium citrate.
7. The cat stress reducing pet body wash according to any one of claims 1-6, further comprising any one or a combination of a chelating agent and a preservative;
preferably, the pet bath cream for reducing the stress response of cats further comprises any one or a combination of two of 0.01-0.5 part of chelating agent and 0.1-1 part of preservative in parts by weight.
8. The preparation method of the pet shower gel for reducing cat stress according to any one of claims 1 to 7, wherein the preparation method comprises the following steps:
(1) Mixing an emulsifier with water to obtain a first mixed solution;
(2) And mixing the first mixed solution with a conditioner, a thickening agent, a humectant and a pH regulator to obtain the water-based emulsion.
9. The method of making a pet shower gel for reducing stress in cats according to claim 8, wherein the water of step (1) is mixed with a chelating agent before being mixed with the emulsifier;
preferably, the step (2) is further mixed with a preservative after mixing.
10. The method for preparing pet shower gel for reducing stress response of cats according to claim 8 is characterized in that the temperature of mixing in step (1) is 80-85 ℃ and the mixing time is 5-20min;
preferably, the mixing temperature in the step (2) is 40-60 ℃, and the mixing time is 5-20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210767937.6A CN115282101B (en) | 2022-06-30 | 2022-06-30 | Pet bath lotion for reducing stress response of cats and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115844755A (en) * | 2022-12-27 | 2023-03-28 | 媞颂日化用品(广州)有限公司 | Tear stain removing gel for pets and preparation method and application thereof |
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| WO2008157847A1 (en) * | 2007-06-20 | 2008-12-24 | The Trustees Of Columbia University In The City Of New York | Skin and surface disinfectant compositions containing botanicals |
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| CN115282101B (en) | 2024-06-25 |
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