CN115340615B - Fluorescent molecule based on cyclodextrin-amino acid and synthetic method and application thereof - Google Patents

Abstract

The invention relates to a fluorescent molecule based on cyclodextrin-amino acid, a synthesis method and application thereof, wherein the preparation method comprises the following steps: adding aldehyde cyclodextrin and amino acid into water to prepare mixed solution, and sequentially adjusting pH, heating and stirring, and carrying out solid-liquid separation to obtain fluorescent molecules based on cyclodextrin-amino acid; wherein the aldehyde cyclodextrin is aldehyde cyclodextrin obtained by pre-oxidizing beta-cyclodextrin with sodium periodate. Compared with the prior art, the cyclodextrin-histidine fluorescent molecule prepared by the invention can be used as a specific recognition fluorescent probe of aureomycin, has high sensitivity and strong selectivity, and can be used for visual portable detection based on fluorescent images.

Description

A fluorescent molecule based on cyclodextrin-amino acid and its synthesis method and application

technical field

The invention belongs to the technical field of fluorescence sensors, and relates to a cyclodextrin-amino acid-based fluorescent molecule, a synthesis method thereof and an application in aureomycin fluorescence detection.

Background technique

Organic fluorescent materials have been widely used in sensors, cell imaging and display technologies. Traditional fluorescent molecules all have the structural basis of chemical bond conjugation based on π-conjugated aromatic structures, which have tunable emission colors and high fluorescence efficiency. However, these materials usually have the characteristics of poor solubility, high biological toxicity, high cost, and complicated synthesis process, which greatly limit their practical application. In contrast, unconventional luminophores without significant conjugated structures have unique advantages of high biocompatibility, low toxicity, good processability, and easy synthesis. Unconventional emitters have electron-rich heteroatoms such as nitrogen (N), oxygen (O), sulfur (S), phosphorus (P), halogens (Cl, Br and I) or contain C=O, C=C and C≡N unsaturated group. They emit in concentrated solutions and/or in the solid state, but tend not to emit light in dilute solutions. This phenomenon is known as cluster triggered emission (CTE). Cluster luminophores in the aggregated state have been used for encryption and bioimaging. However, the concentration of cluster luminophores for cell imaging is 1000 times higher than that of conventional luminophores, which becomes a major obstacle for their practical applications. Furthermore, fluorescent probes need to interact efficiently with analytes for sensing applications. However, current cluster-based solid-state cluster luminescent materials have limited interactions with analytes in aqueous solution, and the reported unconventional luminophores have poor performance as sensors. Therefore, it is highly desirable to find new strategies to promote CTE effects with strong luminescence even in dilute solutions.

Mono-, bis-, oligo-, and polysaccharide crystals with abundant hydroxyl groups have been extensively studied as unconventional emitters. They are weakly emissive in dilute solutions, and bright emission is only observed from concentrated solutions (>8 wt.%) or crystalline state. In dilute solutions, linear polysaccharides, such as sodium alginate, display extended worm-like conformations, which lead to non-luminescence due to lack of sufficient electron delocalization and active molecular motion. Therefore, developing nonlinear molecules and enhancing molecular packing are of great significance for obtaining bright luminescence in dilute solutions.

Compared with traditional detection methods, fluorescent probe technology is considered to be one of the most promising analytical methods for detecting trace pollutants due to its simplicity, portability, high sensitivity, and low cost. At present, tetracycline is a new type of pollutant that is often detected in water, and a variety of fluorescent sensing materials have been reported for the detection of tetracycline antibiotics. However, it is difficult to distinguish tetracycline antibiotics such as chlortetracycline, oxytetracycline, minocycline, tetracycline, etc., whose main molecular structure is very similar. Therefore, it is necessary to design a fluorescent probe for a specific tetracycline antibiotic for development and application.

No new fluorescent molecules based on cyclodextrin-amino acids have been reported yet. Reported technologies such as Chinese patent CN 201810866243.1 disclose a safe and simple method for preparing double-doped nitrogen and phosphorus-carbon quantum dots. In this method, amino acids and carbon precursors (including glucose, citric acid monohydrate or cyclodextrin) Dissolve in deionized water, add phosphoric acid solution, and then undergo ultrasonic treatment for 1-2 hours; heat the sonicated solution in an oil bath at 90-150°C for 1-5 hours to prepare a nitrogen-phosphorus co-doped carbon dot solution. Through patent comparison, although amino acids, cyclodextrin and other raw materials are also used in this patent, the preparation method is obviously different from the final product. The patent uses nitrogen and phosphorus double-doped carbon quantum dots obtained by ultrasonic, high-temperature oil bath, etc. The synthesis time is long and the processing process is complicated. Relatively speaking, the new fluorescent molecule based on amino acid grafted cyclodextrin in the present invention, unlike carbon quantum dots, has a clear molecular composition and spatial structure, the synthesis conditions are mild, the synthesis temperature is 60-80°C, and the reaction time is only 30-60min. In addition, the fluorescent molecule grafted with histidine cyclodextrin can be used as a specific recognition probe of aureomycin, which can realize the portable detection of aureomycin with high sensitivity and high selectivity, showing a good application prospect.

In terms of fluorescence analysis technology of aureomycin, reported technologies such as Chinese patent CN201710302455.2 disclose a gold/platinum bimetallic nanocluster fluorescent probe based on polyethyleneimine protection and its application in the detection of aureomycin. application, this method uses polyethylenimine-protected bimetallic gold/platinum nanoclusters as a fluorescent sensing material based on the mechanism of fluorescence quenching for the detection of ^tetracycline antibiotics; Mechanism, aureomycin can be distinguished from tetracycline, to achieve the purpose of specific detection of aureomycin. It detects aureomycin with a linear range of 0.5-30 μM and a detection limit of 0.5 μM. However, the detection limit of this detection method is still high, and it is difficult to meet the detection requirements of trace aureomycin in existing sewage.

Contents of the invention

The object of the present invention is to provide a fluorescent molecule based on cyclodextrin-amino acid and its synthesis method and application.

The purpose of the present invention can be achieved through the following technical solutions:

A method for synthesizing fluorescent molecules based on cyclodextrin-amino acids, comprising:

Add aldehyde cyclodextrin and amino acid to water to prepare a mixed solution, and after adjusting pH, heating and stirring, and solid-liquid separation in sequence, the fluorescent molecule based on cyclodextrin-amino acid is obtained; the aldehyde cyclodextrin The essence is aldehyde cyclodextrin obtained by preoxidizing β-cyclodextrin with sodium periodate.

Further, the preparation method of described aldehyde cyclodextrin comprises:

β-cyclodextrin and sodium periodate are stirred and reacted in water in the dark, and after nanofiltration, they are stirred and mixed with ethanol until precipitation occurs, followed by filtration, washing, and freeze-drying to obtain aldehyde cyclodextrin;

Wherein, the mol ratio of described β-cyclodextrin and sodium periodate is 1:(1-4);

In the said dark stirring reaction, the reaction temperature is 30-50°C, and the reaction time is 3-5h;

During the purification process, a water/ethanol mixed solution with a volume ratio of 1/4 was used for washing.

Further, the molar ratio of the aldehyde cyclodextrin to amino acid is 1:(2-6).

Further, the amino acids include glycine, isoleucine, methionine, cysteine, glutamic acid, glutamine, asparagine, arginine, lysine, phenylalanine, At least one of tryptophan or histidine.

Further, when the amino acid is at least one of glycine, isoleucine, methionine, cysteine, glutamic acid, glutamine, asparagine, arginine, lysine or phenylalanine One time, adjust the pH of the reaction system to 8-9;

When the amino acid is one or both of tryptophan or histidine, adjust the pH of the reaction system to 6-7.

Further, during the heating and stirring process, the heating temperature is 60-80° C., and the stirring time is 30-60 min.

Further, the solid-liquid separation includes dialysis and freeze-drying.

A fluorescent molecule based on cyclodextrin-amino acid is synthesized by the above-mentioned method.

The application of a cyclodextrin-amino acid-based fluorescent molecule includes using the fluorescent molecule as a fluorescent probe for qualitative and/or quantitative detection of aureomycin in water.

Further, the detection method specifically includes the following steps:

1) Draw a standard curve: Mix the fluorescent probe with a plurality of solutions containing different concentrations of chlortetracycline and stir evenly to obtain a standard solution with a concentration of chlortetracycline in the range of 0-5 μM. Use a 365nm ultraviolet lamp as an excitation light source. Take the fluorescent image in a dark environment, and analyze the B value and G value of the image, take B/G as the vertical axis, and take the aureomycin concentration as the abscissa to draw the standard curve, and the aureomycin detection fitting equation;

2) Detection of aureomycin in the water sample: adopt the same step 1) in the amount ratio of the fluorescent probe and the solution of aureomycin, mix the fluorescent probe with the water sample to be tested to obtain a mixed sample, use a 365nm ultraviolet lamp as the Excite the light source to obtain the fluorescence image, analyze and calculate the B/G of the image, and then obtain the corresponding aureomycin concentration according to the standard curve or fitting equation.

The present invention first uses sodium periodate to oxidize and modify β-cyclodextrin with low solubility and low reactivity to obtain aldehyde-based cyclodextrin with good water solubility, high reactivity and dialdehyde structure; The aldehyde group of the base cyclodextrin reacts with the amino group in various amino acid molecules through Schiff base reaction to obtain a new type of fluorescent molecule based on 12 kinds of amino acids grafted with cyclodextrin of different types of amino acids. The synthesized fluorescent molecules are based on the mechanism of enhanced cluster luminescence, have excellent fluorescent properties in dilute solutions, have good water solubility and excellent environmental compatibility. The synthetic histidine-grafted cyclodextrin fluorescent molecule is used as a fluorescent probe of aureomycin, which can realize a portable detection of aureomycin with high selectivity and high sensitivity based on visualization technology.

Compared with the prior art, the present invention has the following characteristics:

1) The synthesis method of the present invention is simple, obtained by simple modification of cyclodextrin, and the synthesis conditions are relatively mild, the reaction temperature can be controlled below 80°C, and the preparation environment requirements can be provided based on conventional water bath conditions;

2) Compared with traditional fluorescent molecules with conjugated structures, the novel fluorescent molecules of cyclodextrin-amino acids prepared by the present invention have simple structures, low synthesis costs, good water solubility, no biological toxicity, and good environmental compatibility, etc. advantage.

3) The present invention can synthesize a series of cyclodextrin-amino acid novel fluorescent molecules with rich structures and properties, and provides a general method for synthesizing unconventional fluorescent materials based on amino acid-cyclodextrin.

4) The novel fluorescent molecule of cyclodextrin-histidine prepared by the present invention can directly identify the chlortetracycline molecule with high characteristics, and realize the highly sensitive detection of chlortetracycline (the detection range is 0-5 μ M, and the detection limit is 12nM), which is more suitable for the precise detection of low-concentration trace pollutants and high selectivity detection, but it has no signal response to other tetracyclines with very similar molecular main structures. A portable assay for phenotypes.

Description of drawings

Fig. 1 is the three-dimensional fluorescence diagram of the fluorescent molecule of cyclodextrin-amino acid synthesized in embodiment 1 and embodiment 2;

Fig. 2 is the emission spectrogram of the cyclodextrin-amino acid fluorescent molecule synthesized in Example 1 and Example 2 under different excitation wavelength conditions;

Fig. 3 is the fluorescence spectrum of cyclodextrin-histidine under the condition of different amino acid addition amount in embodiment 3;

Fig. 4 is the fluorescence emission spectrum after adding the aureomycin of different concentrations in embodiment 4;

Fig. 5 is the aureomycin detection standard curve drawn in embodiment 4;

Fig. 6 is the effect of different organic small molecules on the fluorescence emission spectrum of the synthesized cyclodextrin-histidine fluorescent molecule in Example 5.

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

A kind of fluorescent molecule based on cyclodextrin-amino acid, its synthesis method comprises the following steps:

S1: Mix β-cyclodextrin and sodium periodate in water at a molar ratio of 1:(1-4), and stir for 3-5 hours at 30-50°C in the dark, then filter with ethanol Stir and mix until the precipitate is separated out, then filter, purify, and freeze-dry in sequence to obtain aldehyde cyclodextrin;

Wherein, the purification process is to use a water/ethanol mixed solution with a volume ratio of 1/4 for washing;

S2: preparing aldehyde cyclodextrin into an aqueous solution;

S3: adding amino acid to the above aqueous solution, adjusting the pH value of the system, heating to 60-80°C, and continuously stirring for 30-60min to obtain the product liquid;

Wherein, the mol ratio of aldehyde cyclodextrin and amino acid is 1:(2-6);

When the amino acid is at least one of glycine, isoleucine, methionine, cysteine, glutamic acid, glutamine, asparagine, arginine, lysine, or phenylalanine , adjust the pH of the reaction system to 8-9;

When the amino acid is one or both of tryptophan or histidine, adjust the pH of the reaction system to 6-7;

S4: The product solution is dialyzed for 12 hours, and the obtained dialysate is freeze-dried to obtain a novel fluorescent material powder based on cyclodextrin-amino acid.

The application of a cyclodextrin-amino acid-based fluorescent molecule includes using the fluorescent molecule as a fluorescent probe for qualitative and/or quantitative detection of aureomycin in water.

Further, the detection method specifically includes the following steps:

1) Draw a standard curve: Mix the fluorescent probe with a plurality of solutions containing different concentrations of chlortetracycline and stir evenly to obtain a standard solution with a concentration of chlortetracycline in the range of 0-5 μM. Use a 365nm ultraviolet lamp as an excitation light source. Fluorescent images were captured in a dark environment, and the image color was uniformized using the software image J, and then the average B value and G value of the image were directly read using the F color picker, with B/G as the ordinate, and the concentration of aureomycin Draw a standard curve for the abscissa, and a chlortetracycline detection fitting equation;

2) Detection of aureomycin in the water sample: adopt the same step 1) in the amount ratio of the fluorescent probe and the solution of aureomycin, mix the fluorescent probe with the water sample to be tested to obtain a mixed sample, use a 365nm ultraviolet lamp as the Excite the light source to obtain the fluorescence image, analyze and calculate the B/G of the image, and then obtain the corresponding aureomycin concentration according to the standard curve or fitting equation.

This embodiment is carried out on the premise of the technical solution of the present invention, and detailed implementation and specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.

Example 1:

A fluorescent molecule based on cyclodextrin-amino acid (histidine/tryptophan), its synthesis method comprises the following steps:

S1: Add 15g of β-cyclodextrin to 100mL of deionized water, and stir evenly, then add 6g of sodium periodate, and stir for 4 hours at 40°C in the dark, filter through a 220nm filter membrane, take the filtrate and Mix it with excess absolute ethanol (800mL) until it precipitates out, then filter, wash with ethanol/water (V/V=80/20) multiple times, and freeze-dry to obtain a dialdehyde structure with good water solubility and high reactivity. Aldehyde cyclodextrin;

S2: Dissolve 0.1mmol (114mg) aldehyde cyclodextrin and 0.6mmol (93mg) histidine or 0.6mmol (82mg) tryptophan in 20mL water to adjust the pH of the system to 6-7;

S3: The above solution was stirred and reacted at 80°C for 60 minutes to obtain cyclodextrin-amino acid fluorescent molecules, which were dialyzed (molecular weight cut-off 1000Da) and freeze-dried to obtain two cyclodextrin-amino acid (histidine/tryptophan) molecules. Acid) fluorescent material powder.

Example 2:

A cyclodextrin-based amino acid (glycine, isoleucine, methionine, cysteine, glutamic acid, glutamine, asparagine, arginine, lysine or phenylalanine ) fluorescent molecule, its synthetic method compared with embodiment 1 difference only lies in:

In step S2, the amino acid used is 0.6mmol (45mg) glycine or 0.6mmol (79mg) isoleucine, 0.6mmol (105mg) arginine, 0.6mmol (79mg) asparagine, 0.6mmol (73mg) cysteine acid, 0.6mmol (88mg) glutamine, 0.6mmol (88mg) glutamic acid, 0.6mmol (88mg) lysine, 0.6mmol (90mg) methionine, 0.6mmol (99mg) phenylalanine; mediation System pH to 8-9;

In step S3, the stirring reaction time is 30 minutes, and finally 10 kinds of fluorescent material powders based on cyclodextrin-amino acid are obtained;

All the other are with embodiment 1.

As shown in FIG. 1 , it is the three-dimensional fluorescence of 12 novel fluorescent molecules (5 mM aqueous solution) based on cyclodextrin-amino acid synthesized in Example 1 and Example 2. It can be seen from the figure that based on the enhanced cluster luminescence effect in the cyclodextrin-confined space, the synthesized cyclodextrin-amino acid novel fluorescent molecule does not have π-conjugated groups, but still has obvious fluorescence Signal, the emission wavelength is concentrated in the range of 350-550nm.

As shown in FIG. 2 , it is the emission spectra of the synthesized 12 cyclodextrin-amino acid-based novel fluorescent molecules (5 mM aqueous solution) at different excitation wavelengths. It can be seen from the figure that the new fluorescent molecules synthesized have excitation wavelength dependence, and the emission peak gradually red shifts as the excitation wavelength increases.

Embodiment 3: the impact of different amino acid additions

A fluorescent molecule based on cyclodextrin-amino acid (histidine), its synthetic method compared with Example 1 only differs in that:

In step S2, the amount of histidine used is 31mg, 62mg, 93mg, 124mg respectively (the molar ratios of aldehyde cyclodextrin and amino acid are 1:2, 1:4, 1:6, 1:8 respectively); the rest are the same Example 1.

Using a 365nm ultraviolet lamp as the excitation light source, the fluorescence emission curve was measured at a concentration of 5mM (aqueous solution). The results are shown in Figure 3. As the amount of histidine added increases, the fluorescence intensity gradually increases. When the added molar ratio is 1:8, the fluorescence intensity does not increase significantly.

Example 4:

In this example, the cyclodextrin-histidine-based fluorescent molecule prepared in Example 1 is used as a fluorescent probe for detecting aureomycin in water samples. The specific detection process is as follows:

1) Draw a standard curve: disperse cyclodextrin-amino acid-based fluorescent molecules into deionized water at a concentration of 200 μM, and use them as fluorescent probes;

Prepare achlortetracycline standard solutions with concentrations of 0, 1, 2, 4, 6, 8, and 10 μM respectively, and mix 0.5 mL each with an equal volume of fluorescent probe to obtain a mixed solution, and use a 365 nm ultraviolet lamp in a dark environment to As an excitation light source, the fluorescence emission curve of the obtained mixed solution was measured, and the results are shown in Figure 4. It can be seen from the figure that as the concentration of aureomycin added increases, the fluorescence intensity of the system gradually increases;

The above mixed solution was photographed to obtain a fluorescence image. As the concentration of aureomycin increased, the brightness of the image gradually increased. Use image analysis software to analyze the B value and G value of the image, with B/G as the ordinate, and Aureomycin as the ordinate. Concentration (μ M) draws standard curve (as shown in Figure 5) for abscissa, and obtains aureomycin detection fitting equation: y=0.1431x+1.3319, (R2=0.998); According to limit of detection LOD=3σ/N (wherein, σ is the standard deviation of the blank sample, and N is the slope of the linear equation). It is calculated that the detection limit of the probe is 12nM using the fluorescent molecule of cyclodextrin-histidine.

2) Pretreatment of the water sample to be tested: filter the water sample to be tested and adjust its pH to be neutral;

3) Detection of aureomycin in water samples: take 0.5mL pretreated water sample, mix with 0.5mL fluorescent probe solution to obtain a mixed sample, use 365nm ultraviolet lamp as excitation light source to obtain fluorescence image and use image analysis software to obtain image B/G, and then according to the fitting equation, the corresponding concentration of aureomycin was obtained. The campus water and tap water of Tongji University (Siping Road Campus) were collected for standard recovery experiments, adding 1 μM, 3 μM, 4 μM and 7 μM aureomycin respectively, and using the above method to test, the recovery rate of aureomycin was 97.25%-101.67 %, the specific results are shown in Attached Table 1.

Table 1 The results of the standard recovery experiment

Example 5:

Using the cyclodextrin-histidine synthesized in Example 1 as a fluorescent probe to selectively detect different coexisting or structurally similar small molecular organic compounds, the specific method is as follows:

Disperse fluorescent molecules based on cyclodextrin-amino acids in deionized water at a concentration of 200 μM, and use them as fluorescent probes; at the same time, prepare multiple 200 μM solutions of different small molecule organic substances, including aureomycin (CTC), acetic acid (AC) and glucose (Glucose), ampicillin (Amp), chloramphenicol (CAP), streptomycin (Str), nalidixic acid (Nal), glycine (Gly), histidine (His), phenylalanine (Phe) , tryptophan (Trp), trichloroacetamide (TCAM), tetracycline (TC), oxytetracycline (OTC), minocycline (MOC), and mix 1 mL each with an equal volume of fluorescent probe solution, After standing still until the reaction system is stable, test its fluorescence spectrum (with a 365nm ultraviolet lamp as the excitation light source) to investigate its selective recognition ability. As shown in Figure 6, after adding aureomycin, the fluorescence signal of the system is significantly enhanced, and simultaneously The peak blue shifted, but the fluorescence response of the system did not change significantly after the addition of other small molecular organic substances, indicating that the method had good selectivity and realized the specific recognition of aureomycin by the cyclodextrin-histidine fluorescent probe.

The above descriptions of the embodiments are for those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments, and apply the general principles described here to other embodiments without creative effort. Therefore, the present invention is not limited to the above-mentioned embodiments. Improvements and modifications made by those skilled in the art according to the disclosure of the present invention without departing from the scope of the present invention should fall within the protection scope of the present invention.

Claims (8)

1. A method for synthesizing a fluorescent molecule based on cyclodextrin-amino acid, comprising:

adding aldehyde cyclodextrin and amino acid into water to prepare mixed solution, and sequentially adjusting pH, heating and stirring, and carrying out solid-liquid separation to obtain fluorescent molecules based on cyclodextrin-amino acid; wherein the aldehyde cyclodextrin is aldehyde cyclodextrin obtained by pre-oxidizing beta-cyclodextrin by sodium periodate;

the preparation method of the aldehyde cyclodextrin comprises the following steps:

stirring beta-cyclodextrin and sodium periodate in water in a dark place for reaction, stirring and mixing the mixture with ethanol after nanofiltration until precipitation is achieved, and then filtering, washing and freeze-drying the mixture in sequence to obtain aldehyde cyclodextrin;

wherein, the mol ratio of the beta-cyclodextrin to the sodium periodate is 1 (1-4);

in the light-shielding stirring reaction, the reaction temperature is 30-50 ℃ and the reaction time is 3-5h;

the molar ratio of the aldehyde cyclodextrin to the amino acid is 1 (2-6).

2. The method of claim 1, wherein the amino acid comprises at least one of glycine, isoleucine, methionine, cysteine, glutamic acid, glutamine, asparagine, arginine, lysine, phenylalanine, tryptophan, or histidine.

3. The method for synthesizing a cyclodextrin-amino acid-based fluorescent molecule according to claim 2, wherein when the amino acid is at least one of glycine, isoleucine, methionine, cysteine, glutamic acid, glutamine, asparagine, arginine, lysine or phenylalanine, the pH of the reaction system is adjusted to 8-9;

when the amino acid is one or two of tryptophan and histidine, the pH of the reaction system is adjusted to 6-7.

4. The method for synthesizing fluorescent molecules based on cyclodextrin-amino acid according to claim 1, wherein the heating temperature is 60-80 ℃ and the stirring time is 30-60min during the heating and stirring process.

5. The method for synthesizing a fluorescent molecule based on cyclodextrin-amino acids according to claim 1, wherein the solid-liquid separation comprises dialysis and freeze-drying.

6. A cyclodextrin-amino acid based fluorescent molecule synthesized by the method of any one of claims 1 to 5.

7. The use of a fluorescent molecule based on cyclodextrin-amino acids according to claim 6, wherein said fluorescent molecule is used as a fluorescent probe for qualitative and/or quantitative detection of aureomycin in a body of water.

8. The use of a cyclodextrin-amino acid based fluorescent molecule according to claim 7, wherein the detection method comprises the steps of:

1) Drawing a standard curve: respectively mixing and uniformly stirring the fluorescent probes with a plurality of solutions containing aureomycin with different concentrations to obtain a standard solution with aureomycin concentration range of 0-5 mu M, taking a 365nm ultraviolet lamp as an excitation light source, shooting in a dark environment to obtain a fluorescent image, analyzing the B value and the G value of the image, drawing a standard curve with B/G as an ordinate and aureomycin concentration as an abscissa, and detecting and fitting an equation of aureomycin;

2) Detecting aureomycin in a water sample: mixing the fluorescent probe with a water sample to be detected by adopting the dosage ratio of the fluorescent probe to the solution of the aureomycin in the step 1) to obtain a mixed sample, obtaining a fluorescent image by taking a 365nm ultraviolet lamp as an excitation light source, analyzing and calculating to obtain B/G of the image, and obtaining the corresponding aureomycin concentration according to a standard curve or a fitting equation.

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