CN115846048B - Circulating magnetic field dynamic magnetophoresis separation device and method - Google Patents
Circulating magnetic field dynamic magnetophoresis separation device and method Download PDFInfo
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Abstract
The invention provides a circulating magnetic field dynamic magnetophoresis separation device and a method, wherein a linear micro-pipe separation channel is arranged at the upper part of a linear base to form an open structure, and a linear circulating magnetic field enables magnetic particles to move along a straight line in the linear separation channel integrally to finish dynamic magnetophoresis separation of the magnetic particles. The external magnetic field source is optimized, and the magnetic particles in the linear separation channel are subjected to dynamic magnetophoresis by adopting an open structure. The test instrument is beneficial to being placed around the separation channel, and the situation that the magnetic particles are separated in the separation channel in real time is observed and monitored. Compared with a spiral separation channel, the linear separation channel is easy to process and fix. According to the circulating magnetic field dynamic magnetophoresis separation method provided on the basis of the device, after the magnetic particles flow into the separation channel, the carrier liquid is controlled not to flow, and the magnetic particles with different characteristics realize separation through magnetophoresis movement under the traction effect of the magnetic field force of the circulating magnetic field, so that the influence of the carrier liquid flow velocity on the magnetophoresis separation is reduced.
Description
Technical Field
The invention relates to the technical field of magnetic particle and magnetic labeling biological molecule positive magnetophoresis and non-magnetic particle negative magnetophoresis separation analysis, in particular to a circulating magnetic field dynamic magnetophoresis separation device and a circulating magnetic field dynamic magnetophoresis separation method.
Background
The magnetophoresis separation of magnetic particles, magnetic labeling particles and biomolecules is applied in analytical chemistry, environmental detection, bioengineering, clinical medicine and other fields, and is used for analyzing components of substances, measuring the content of certain components, controlling genetic materials and controlling the quality of samples. In addition, when the carrier liquid around the nonmagnetic particles is magnetic fluid, the nonmagnetic particles may be separated by a negative magnetophoresis method. The magnetophoresis separation can separate not only magnetic particles but also non-magnetic particles, and is widely applied to particle separation.
In the dynamic magnetophoresis separation of a three-phase winding rotating magnetic field, as in the patent 202111498900.X 'magnetic particle dynamic magnetophoresis separation device and method based on rotating magnetic field' of the prior application of the inventor, a cylindrical base with a hollow structure is provided with a three-phase winding with an oblique slot, after three-phase sinusoidal alternating current is connected, a rotating magnetic field is generated in a cylindrical space with the hollow structure formed by the three-phase winding and the base, a micro-pipe separation channel is made into a spiral shape and is placed in the cylindrical space, the rotating magnetic field and the spiral separation channel are mutually matched, and magnetic particles in the channel are subjected to the action of periodical magnetic field force and magnetophoresis movement along the rotating direction of the rotating magnetic field. The rotating magnetic field generated by the three-phase winding of the oblique slot makes the magnetic particles with different characteristics in the separation channel have different magnetic force component forces along the flow direction of the carrier liquid, so that the magnetophoresis speed difference can be generated, and the effective separation is realized.
The external magnetic field used for magnetophoresis separation is a rotating magnetic field in the cylindrical hollow structure of the base, and the separation channel is a spiral micro-tubule. The base of the hollow cylindrical structure is adopted, the inner wall of the base is provided with a lower wire slot, the three-phase winding is embedded into the lower wire slot, and a spiral micro tube is arranged in the cylindrical space to serve as a separation channel.
Although effective magnetophoretic separation can be realized, the scheme has the following problems that (1) under the action of a rotating magnetic field, magnetic particles move along a spiral separation channel, and the movement track is relatively complex. (2) The magnetic separation device is of a closed structure, the separation channel is arranged in the cylindrical structure, and under the transparent condition of the micro-pipe separation channel, the real-time magnetophoresis movement condition of magnetic particles in the separation channel is not convenient to place and observe and record by a test instrument. (3) The cylindrical spiral separation channel, if the lead or diameter of the spiral needs to be changed, needs to reprocess the spiral fixing groove of the separation channel, which brings inconvenience to the separation work. (4) In the magnetophoresis separation method of the rotating magnetic field dynamic magnetophoresis separation device, an infusion pump is always started in the magnetic particle separation process, and the flow rate of the carrier liquid and the magnetophoresis movement of the magnetic particles are mutually overlapped, so that a certain influence can be caused on the particle separation result.
Disclosure of Invention
In view of the above, the present invention provides a circulating magnetic field dynamic magnetophoresis separation device and method, so as to solve the technical problems that the motion track of magnetic particles is complex, the real-time magnetophoresis motion condition of magnetic particles in a separation channel cannot be observed and recorded, the size change of the separation channel cannot be dealt with, and an accurate particle separation result cannot be obtained when the conventional dynamic magnetophoresis separation device performs magnetophoresis separation.
For this purpose, the invention provides the following technical scheme:
The invention provides a dynamic magnetophoresis separation device of a circulating magnetic field, which comprises a base, a separation channel, a three-phase winding, a three-phase transformer, a frequency conversion circuit, a UV/Vi ultraviolet/visible light detector, a waste liquid collecting bottle, a three-way valve and an infusion pump, wherein the base is a linear base, a wire slot is arranged on the base, the three-phase winding is embedded in the wire slot, when three-phase alternating current is introduced into the three-phase winding, a moving magnetic field is generated at the upper part of the linear base, the magnetic induction intensity of the magnetic field changes according to the rule of a sine function curve, and when three-phase current changes periodically, the circulating magnetic field is formed at the upper part of the base;
The separation channel is a linear separation channel and is tubular, the separation channel is positioned at the upper part of the base, and when the circulating magnetic pole moves to pass near the magnetic particles in the separation channel, the particles are subjected to the action of magnetic field force to generate magnetophoretic motion.
Further, the device also comprises a testing instrument arranged around the separation channel.
Further, the base is a three-phase winding base structure with 4 poles, the 4 poles generated by the three-phase winding are two pairs of N poles and S poles, the magnetic field at the upper part of the base advances and circulates along the straight line direction according to the distribution of the N poles, the S poles, the N poles and the S poles, and the spatial phase difference is 90 degrees in sequence.
Further, the base is a silicon steel base.
Further, the device comprises a three-phase winding base, wherein a transparent acrylic plate is fixed on the upper part of the base, a separation channel positioning groove is formed in the acrylic plate, and the separation channel is arranged in the positioning groove in an embedded mode.
The device further comprises two three-phase winding bases which are placed in a mirror symmetry mode up and down, two transparent acrylic plates with the same specification are fixed between the two bases to form a combining surface, a semicircular column groove is formed in the lower combining surface, a semicircular column groove is formed in the upper combining surface, and a separation channel is arranged in a positioning groove formed by the two semicircular column grooves in an embedded mode.
In still another aspect, the present invention further provides a method for separating magnetic particles by using the above-mentioned circulating magnetic field dynamic magnetophoretic separation device, including:
preparing magnetic particles with the same magnetic susceptibility and different particle sizes;
starting an infusion pump, injecting the magnetic particles to be separated into the carrier liquid through a three-way valve by using a sample injection injector, and closing the infusion pump when the magnetic particles flow into the separation channel under the drive of the carrier liquid, so that the flow speed of the carrier liquid is zero;
The frequency, magnetic induction intensity and carrier fluid flow parameters of the circulating magnetic field are adjusted to carry out magnetic particle magnetic field force traction magnetophoresis separation;
After the magnetic particles are subjected to magnetophoresis separation in the separation channel, the power supply of the linear three-phase winding base is disconnected, the circulating magnetic field disappears, the infusion pump is started, and the carrier liquid flows out of the separation channel with the magnetic particles at a certain flow rate.
Further, the method comprises the step of observing the separation of the particles through a transparent separation channel by using a testing instrument.
Further, the method also comprises the step of testing the separation effect by using an ultraviolet-visible light detector.
The invention has the advantages and positive effects that:
The circulating magnetic field dynamic magnetophoresis separation device of the invention places the linear micro-pipe separation channel on the upper part of the linear base to form an open structure, thereby realizing magnetophoresis separation of magnetic particles with different characteristics. The linear circulating magnetic field enables the magnetic particles to move along the straight line in the separation channel as a whole, and dynamic magnetophoresis separation of the magnetic particles can be completed in the linear separation channel. The external magnetic field source is optimized, and the magnetic particles in the linear separation channel are subjected to dynamic magnetophoresis by adopting an open structure. The test instrument is beneficial to being placed around the separation channel, and the situation that the magnetic particles are separated in the separation channel in real time is observed and monitored. Compared with a spiral separation channel, the linear separation channel is easy to process, easy to fix and convenient to develop magnetophoresis separation work.
The magnetophoresis separation method based on the dynamic magnetophoresis separation device of the circulating magnetic field is to turn off the liquid conveying pump to control the carrier liquid to flow after the magnetic particles flow into the separation channel, separate the magnetic particles with different characteristics through magnetophoresis movement under the traction of the magnetic force of the circulating magnetic field, turn on the liquid conveying pump, and drive the separated magnetic particles to flow out of the separation channel in time sequence with the carrier liquid. This approach reduces the effect of carrier fluid flow rate on magnetophoretic separation.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to the drawings without inventive effort to a person skilled in the art.
FIG. 1 is a schematic diagram of a circulating magnetic field dynamic magnetophoretic separation device according to an embodiment of the invention;
FIG. 2 is a schematic view of a linear base structure in accordance with an embodiment of the present invention;
FIG. 3 is a schematic diagram of the generation of a linear circulating magnetic field in an embodiment of the present invention;
FIG. 4 is a single-layer chain connection diagram of a three-phase winding of a linear base in an embodiment of the invention;
FIG. 5 is a schematic diagram of a separation channel in an embodiment of the present invention;
FIG. 6 is a schematic diagram of a three-phase winding base and a split channel structure according to an embodiment of the present invention;
FIG. 7 is a schematic view of a three-phase winding base and a split channel structure according to another embodiment of the present invention;
FIG. 8 is a schematic diagram of the separation results of the dynamic magnetophoretic separation of a circulating magnetic field in an embodiment of the invention;
In the figure, a 1-base, a 2-separation channel, a 3-three-phase winding, a 3-1-A phase winding, a 3-2-B phase winding, a 3-3-C phase winding, a 4-three-phase transformer, a 5-frequency conversion circuit, a 6-UV/Vi ultraviolet/visible light detector, a 7-waste liquid collecting bottle, an 8-three-way valve, a 9-infusion pump, a 10-wire slot, an 11-equivalent magnetic pole, a 12-acrylic plate, a 13-separation channel positioning slot, a 14-carrier liquid inlet, a 15-carrier liquid outlet, a 16-sample injection syringe and a 17-magnetic induction intensity.
Detailed Description
In order that those skilled in the art will better understand the present invention, a technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present invention and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the invention described herein may be implemented in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
As shown in FIG. 1, the circulating magnetic field dynamic magnetophoresis separation device in the embodiment of the invention comprises a base 1, a separation channel 2, a three-phase winding 3, a three-phase transformer 4, a frequency conversion circuit 5, a UV/Vi ultraviolet/visible light detector 6, a waste liquid collecting bottle 7, a three-way valve 8 and an infusion pump 9.
After the three-phase winding 3 is connected with three-phase sinusoidal alternating current, a circulating magnetic field is generated at the upper part of the base 1, and the linear micro-pipe separation channel 2 is arranged at the upper part of the base 1. Under the combined action of the sample injection injector 16, the three-way valve 8 and the infusion pump 9, a sample is input into the separation channel 2, and magnetic particles in sample injection move in the separation channel 2 in a straight line under the drive of carrier liquid. The three-phase winding base generating the circulating magnetic field and the linear separation channel support each other, and when the circulating magnetic field passes near the magnetic particles in the separation channel, the particles are subjected to the magnetic force generated by the circulating magnetic field and magnetically move at a certain speed along the moving direction of the magnetic field. The carrier liquid flowing out of the separation channel 2 is detected by a UV/Vi ultraviolet/visible light detector, and the waste liquid is recovered by a waste liquid collecting bottle.
The three-phase winding base structure is externally connected with a frequency conversion circuit 5, and the frequency conversion circuit 5 can adjust the frequency of the three-phase alternating voltage applied to the three-phase winding 3, so that the frequency of the action of the circulating magnetic field on the magnetic particles in the separation channel is controlled. The three-phase winding base structure is externally connected with a three-phase transformer 4, the three-phase transformer 4 can change the magnitude of three-phase alternating voltage applied to the three-phase winding 3, influence the magnitude of magnetic induction intensity in the separation channel 2, and control the magnetic field force born by the magnetic particles in the separation channel 2. The circulating magnetic field generated by the three-phase winding base enables magnetic force component forces of magnetic particles with different characteristics to be different along the flowing direction of carrier liquid, and magnetic electrophoresis speed difference is generated, so that effective separation of the magnetic particles is realized.
As shown in fig. 2, the base 1 in the embodiment of the present invention is a silicon steel linear base, the base 1 is provided with a lower wire slot 10, and the three-phase winding 3 is embedded in the lower wire slot 10. When three-phase alternating current is introduced into the three-phase winding 3, a moving magnetic field is generated at the upper part of the linear silicon steel base 1, and the magnetic induction intensity of the magnetic field changes according to a sine function curve rule. The three-phase current is U phase, V phase and W phase respectively, the pole pair number p=1 is set, and the phase of the U phase current is at ωt=0 DEGThe magnetic field generated at the upper part of the linear base at ωt=150° is shown in fig. 3. At this instant U-phase current i 1 >0, v-phase current i 2 >0, and w-phase current i 3 <0, which corresponds to an equivalent pole S-pole 11 in the upper portion of base 1. The three-phase alternating current continues to change and the S pole travels to the left, the magnetic induction 17 being shown in dashed lines. The three-phase current changes for one period, and the magnetic pole S pole passes through the upper part of the base once from right to left along a straight line. Thus, when the three-phase current is periodically changed, a circulating magnetic field is formed at the upper portion of the susceptor 1.
For a 4-pole three-phase winding base structure, it is equivalent to 4 poles traveling in a straight line at the upper portion of the base 1. The three-phase winding 3 comprises an A-phase winding 3-1, a B-phase winding 3-2 and a C-phase winding 3-3, the generated 4 poles are two pairs of N pole and S pole magnetic poles, the magnetic field at the upper part of the base advances and circulates along the straight line direction according to the distribution of N pole-S pole-N pole-S pole, and the spatial phase differences are 90 degrees in sequence. A single-layer chained connection of three-phase windings with 4 poles and 24 slots to generate a circulating magnetic field is shown in fig. 4.
The separation channel is made of PEEK plastic, the linear separation channel is shown in fig. 5, a transparent acrylic plate 12 is fixed on the upper portion of a silicon steel base, a separation channel positioning groove 13 is formed in the acrylic plate 12, the separation channel 2 is tubular, the outer diameter is 1.59mm, the inner diameter is 0.75mm, the separation channel is directly embedded in the positioning groove 13, a carrier liquid inlet 14 is formed in the left side, and a carrier liquid outlet 15 is formed in the right side. When the circulating magnetic pole moves through the vicinity of the magnetic particles in the separation channel, the particles are subjected to the action of the magnetic field force, and magnetophoretic movement occurs.
Three-phase winding base and split channel structure example 1:
The three-phase winding base and split channel structure of example 1 is shown in fig. 6. A three-phase winding silicon steel base 1, the upper portion of the base fixes the transparent acrylic plate 12, there are locating slots 13 of separation channel on acrylic plate 12, the separation channel 2 is set up in the locating slot 13 in an embedded way. In the unilateral structure, the magnetic particles in the separation channel bear larger normal magnetic pulling force generated by the circulating magnetic field.
Three-phase winding base and split channel structure example 2:
the three-phase winding base and split channel structure of example 2 is shown in fig. 7. The two three-phase winding silicon steel bases 1 are placed in a mirror symmetry mode up and down. Two transparent acrylic plates 12 with the same specification are fixed between the bases. On the bonding surface of the acrylic plate 12, a linear separation channel 2 is provided, specifically, a lower bonding surface is provided with a semicircular column groove, an upper bonding surface is also provided with a semicircular column groove, and a tubular separation channel is embedded in a positioning groove formed by the two semicircular column grooves. The magnetic particles in the separation channel bear the normal magnetic pulling force of the circulating magnetic field and can be mutually offset.
The linear three-phase winding base dynamic magnetophoresis device can be used for carrying out magnetic particle dynamic magnetophoresis separation, and the specific method comprises the following steps:
S1, preparing magnetic particles with the same magnetic susceptibility and different particle sizes.
S2, starting an infusion pump, injecting the magnetic particles to be separated into the carrier liquid through a three-way valve by using a sample injection syringe, and closing the infusion pump when the magnetic particles flow into the separation channel under the drive of the carrier liquid, so that the flow rate of the carrier liquid is zero.
S3, switching on a power supply of the linear three-phase winding base, generating a circulating magnetic field at the upper part of the base, adjusting parameters such as frequency and magnetic induction intensity of the circulating magnetic field, carrier liquid flow speed and the like, and carrying out magnetic particle magnetic field force traction magnetophoresis separation;
Under the action of the linear motion circulating magnetic field, when the equivalent magnetic pole passes near the magnetic particles in the separation channel, the particles are pulled by the magnetic field force of the circulating magnetic field, and the magnetophoretic motion is generated along the flow direction of the carrier liquid, so as to generate magnetophoretic displacement.
S4, observing the separation condition of the particles through a transparent separation channel by using a testing instrument such as a microscope.
Magnetic particles of different characteristics are subjected to different magnetic force components in the flow direction of the carrier liquid, so that magnetophoretic velocity differences are generated, magnetophoretic displacements are different, and the positions in the separation channel are in front of and behind.
S5, after the magnetic particles are subjected to magnetophoresis separation in the separation channel, the power supply of the linear three-phase winding base is disconnected, the circulating magnetic field disappears, the infusion pump is started, and the carrier liquid flows out of the separation channel with the magnetic particles at a certain flow rate.
S6, testing the separation effect by using an ultraviolet-visible light detector (UV/Vis).
The retention time of the magnetic particles with different characteristics in the separation channel is different, and the time before and after the magnetic particles flow out of the separation channel is different, so that the magnetophoresis separation is realized.
For ease of understanding, the above-described magnetophoretic separation method will be described below with a specific example.
On a circulating magnetic field dynamic magnetophoretic separation device, in particular according to example 2, magnetic force of a circulating magnetic field along a linear motion is used to pull magnetophoretic motion for separating magnetic particles in a separation channel, and magnetic particles with different particle sizes are separated. Core-shell polystyrene magnetic particles with different particle sizes are prepared, and a mixed sample with the magnetic particle diameters of 6.3 mu m and 3.5 mu m is obtained. The magnetic particles with different particle diameters have the same magnetic susceptibility and exhibit superparamagnetic response characteristics. Setting the working state and related parameters of the three-phase winding circulating magnetic field magnetophoresis separation device. The travelling direction of the circulating magnetic field is the same as the flowing direction of the carrier liquid, and the sample feeding amount of the magnetic particle mixed sample is 20 μl. The frequency of the rotating magnetic field is regulated to 6Hz, the three-phase voltage applied to the three-phase winding is 18V, deionized water is used as carrier liquid, and the carrier liquid flow rate is 0.1ml/min. And starting an infusion pump, injecting the magnetic particle mixed sample into the carrier liquid through the three-way valve, and closing the infusion pump after the mixed sample flows into the separation channel under the drive of the carrier liquid. When the power supply of the linear three-phase winding base is connected, and the circulating magnetic field linearly passes near the magnetic particles in the separation channel, the particles are pulled by the magnetic field force of the circulating magnetic field, and magnetophoretic displacement occurs along the flow direction of the carrier liquid. The magnetophoretic displacements of the magnetic particles with different characteristics are different, and the positions in the separation channel are in front and back. After 12 minutes, the magnetic particles realize magnetophoresis separation in the separation channel, the power supply of the linear three-phase winding base is disconnected, the circulating magnetic field disappears, the infusion pump is started, and the separated magnetic particles are driven by the carrier liquid to flow out of the separation channel and pass through the ultraviolet-visible light detector. The separation was tested with an ultraviolet-visible light detector and the separation of the magnetic particles was as shown in fig. 8. Magnetic particles with a diameter of 6.3 μm (peak a) and 3.5 μm (peak b) achieved separation. The two magnetic particles with the same magnetic susceptibility and different diameters can be completely separated in the circulating magnetic field dynamic magnetophoresis separation device, so as to meet the separation analysis requirement. The circulating magnetic field dynamic magnetophoresis separation method realizes the separation of magnetic particles and has good resolution.
It should be noted that the above embodiments are merely for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that the technical solution described in the above embodiments may be modified or some or all of the technical features may be equivalently replaced, and these modifications or substitutions do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the present invention.
Claims (8)
1. A dynamic magnetophoresis separation device of a circulating magnetic field comprises a base, a separation channel, a three-phase winding, a three-phase transformer, a frequency conversion circuit, a UV/Vi ultraviolet/visible light detector, a waste liquid collecting bottle, a three-way valve and an infusion pump, and is characterized in that the base is a linear base, a lower wire slot is arranged on the base, the three-phase winding is embedded into the lower wire slot to form a linear arrangement of three-phase windings;
The separation channel is a linear separation channel and is tubular; when the circulating magnetic pole moves to pass near the magnetic particles in the separation channel, the particles are acted by magnetic force to pull the magnetic particles to perform magnetophoretic movement;
The magnetic particles with the same magnetic susceptibility and different particle diameters are prepared when the magnetic particles are subjected to dynamic magnetophoresis separation by the circulating magnetic field dynamic magnetophoresis separation device, an infusion pump is started, the magnetic particles to be separated are injected into a carrier liquid by a sample injection syringe through a three-way valve, when the magnetic particles are driven by the carrier liquid to flow into a separation channel, the infusion pump is closed to enable the flow speed of the carrier liquid to be zero, a power supply of a linear three-phase winding base is connected, a circulating magnetic field is generated at the upper part of the base, the frequency, the magnetic induction intensity and the carrier liquid flow speed parameters of the circulating magnetic field are regulated, the magnetic particle magnetic field force traction magnetophoresis separation is carried out, when the magnetic particles with different characteristics are subjected to magnetophoresis separation in the separation channel at different speeds, the power supply of the linear three-phase winding base is disconnected after the magnetophoresis separation is realized through the speed difference, the circulating magnetic field disappears, the infusion pump is opened, and the carrier liquid carries the magnetic particles with different characteristics at a certain flow speed to flow speed and flow out of the separation channel successively.
2. A circulating magnetic field dynamic magnetophoretic separation device according to claim 1 further comprising a test instrument arranged around said separation channel.
3. The dynamic magnetophoretic separation device according to claim 1 wherein the base is a 4-pole three-phase winding base structure, the 4 poles generated by the three-phase winding are two pairs of N-pole and S-pole magnetic poles, the magnetic field at the upper part of the base travels and circulates in a straight line direction in a distribution of N-pole, S-pole, N-pole and S-pole, and the spatial phase differences are sequentially 90 °.
4. The circulating magnetic field dynamic magnetophoretic separation device of claim 1 wherein the base is a silicon steel base.
5. The dynamic magnetophoretic separation device according to claim 4 wherein the device comprises a three-phase winding base, a transparent acrylic plate is fixed on the upper part of the base, a separation channel positioning groove is formed in the acrylic plate, and the separation channel is arranged in the positioning groove in an embedded manner.
6. The dynamic magnetophoretic separation device according to claim 4 wherein the device comprises two three-phase winding bases which are placed in mirror symmetry up and down, two transparent acrylic plates of the same specification are fixed between the two bases to form a joint surface, a semi-cylindrical groove is formed on the lower joint surface, a semi-cylindrical groove is formed on the upper joint surface, and separation channels are arranged in a positioning groove formed by the two semi-cylindrical grooves in an embedded mode.
7. A circulating magnetic field dynamic magnetophoretic separation device according to claim 1 further comprising observing separation of particles through the transparent separation channel using the test instrument.
8. The dynamic magnetophoretic separation device according to claim 1 further comprising testing the separation effect with an ultraviolet-visible light detector.
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| KR100394491B1 (en) * | 2000-02-04 | 2003-08-09 | 하루오 고지마 | Method for separating magnetic particles and separating system |
| WO2005072854A1 (en) * | 2004-02-02 | 2005-08-11 | The University Of British Columbia | Scodaphoresis and methods and apparatus for moving and concentrating particles |
| KR100791036B1 (en) * | 2006-07-07 | 2008-01-03 | 한국과학기술원 | Separation method of pure carbon nanotubes and carbon nanotubes containing metal impurities using continuous magnetophoresis and magnetophoretic microfluidic control device |
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| DE102010010220A1 (en) * | 2010-03-03 | 2011-09-08 | Siemens Aktiengesellschaft | Separator for separating a mixture |
| KR20120026959A (en) * | 2010-09-10 | 2012-03-20 | 인제대학교 산학협력단 | Microparticle separator based on magnetophoresis and microparticle separating method using the same |
| KR101636316B1 (en) * | 2014-09-22 | 2016-07-05 | 연세대학교 산학협력단 | Microfluidic apparatus for isolation, method for isolation using the same, and isolation kit for circulating rare cells using the same |
| CN109529961B (en) * | 2019-01-04 | 2020-11-13 | 大连理工大学 | Micro-fluidic device for converging micro-nano biological particles by utilizing oscillatory flow and negative magnetophoresis effect |
| CN111235224B (en) * | 2020-01-14 | 2023-06-20 | 广东工业大学 | A method and device for precise modification of biomolecules based on magnetophoretic separation |
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| CN112779221A (en) * | 2019-11-07 | 2021-05-11 | 北京机械设备研究所 | Separation method based on circulating tumor cell forward separation system |
| CN114345545A (en) * | 2021-12-09 | 2022-04-15 | 大连交通大学 | Device and method for dynamic magnetophoretic separation of magnetic particles based on rotating magnetic field |
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