CN115927443A - Regulation and control of (Z) -3-hexenol and application thereof in drought resistance of tea trees - Google Patents

Abstract

The invention discloses a regulation and control method of (Z) -3-hexenol and application thereof in tea tree drought resistance, wherein the (Z) -3-hexenol with the working concentration of 0.01-0.5 mu g/L is added on tea seedlings for slow release and drought treatment, and the drought resistance of tea trees can be effectively improved. The CsADH gene is overexpressed in the tobacco, the (Z) -3-hexenol content in the tobacco is increased, and the drought resistance of tobacco plants is improved; the tea tree silences a CsADH gene, reduces the (Z) -3-hexenol content in the tea tree, and reduces the drought resistance of the tea tree. The synthesis of (Z) -3-hexenol can be effectively regulated and controlled by regulating the expression quantity of the CsADH gene, and the drought resistance of tea trees can be regulated and controlled. Only one component is needed to act on the (Z) -3-hexenol independently, the use concentration is low, the acting force is strong, the cost is low, and the (Z) -3-hexenol is a natural product in the nature, is volatilized and naturally degraded, and is non-toxic and harmless to the environment.

Description

Regulation of (Z)-3-hexenol and its application in drought resistance of tea plants

technical field

The invention belongs to the field of biotechnology, and in particular relates to the regulation of (Z)-3-hexenol and its application in drought resistance of tea trees.

Background technique

Tea tree (Camellia sinensis) is a perennial evergreen woody plant that likes temperature and humidity, but has weak drought tolerance. Like other crops, drought has become one of the main abiotic stresses restricting tea production. With global warming and increasing water scarcity, this problem has become more serious. According to reports, drought stress can reduce tea production by 14%~ 33%, and the death rate of tea plants is increased by 6% to 19%, which has brought serious economic losses to the tea industry.

At present, the drought-resistant technologies of tea gardens mainly include: timely irrigation, covering between tea rows, reducing pruning, timely fertilization, and building shading facilities. Volatile substances have many physiological functions, including improving the stress resistance of plants. (Z)-3-hexenol in tea tree is synthesized from (Z)-3-hexenal under the catalysis of alcohol dehydrogenase (ADH). The gene CsADH encoding ADH has been found in tea tree. (Z)-3-Hexenol plays a very important role in the direct defense of tea trees, mainly used to defend against potential pests. Research on improving the drought resistance of tea trees is relatively rare.

Based on the above analysis, a method to deal with drought resistance of tea trees by regulating volatile substances is urgently needed in the industry.

Contents of the invention

In view of the above deficiencies, the present invention provides a method to deal with drought resistance of tea plants by regulating (Z)-3-hexenol, so as to improve the drought resistance ability of tea seedlings and seek new ways for traditional drought resistance technologies.

The present invention is realized by following technical means:

The invention discloses the application of (Z)-3-hexenol in drought resistance of tea trees.

Further, the working concentration of (Z)-3-hexenol is 0.01-0.5 μg/L.

Furthermore, adding (Z)-3-hexenol at a working concentration of 0.01-0.5 μg/L to the tea seedlings for slow release, and treating them for 12 hours, followed by drought treatment, can improve the drought resistance of the tea seedlings.

The invention also discloses a gene that can increase the content of (Z)-3-hexenol in tobacco through overexpression, wherein the gene is CsADH gene.

Further, the CsADH gene is overexpressed, the content of (Z)-3-hexenol is increased, and the drought resistance ability of the plant is improved.

The invention also discloses a gene whose silent expression can reduce the content of (Z)-3-hexenol in tea tree, wherein the gene is CsADH gene.

Furthermore, the present invention also discloses an application of the above-mentioned gene in constructing a tea plant strain with low (Z)-3-hexenol content.

Further, silencing the CsADH gene in the tea tree reduces the content of (Z)-3-hexenol in the tea tree and reduces the drought resistance of the tea tree.

The beneficial effects of the present invention are:

1. The present invention uses (Z)-3-hexenol to pretreat tea trees, which can effectively improve the drought resistance of tea trees.

2. By regulating the expression of CsADH gene, the synthesis of (Z)-3-hexenol can be effectively regulated, and the drought resistance ability of tea plants can be regulated.

3. Only one ingredient is needed to act alone (Z)-3-hexenol. The concentration is low, the action is strong, and the cost is low. (Z)-3-Hexenol is a natural product in nature. The aroma volatilizes and degrades naturally, which is harmful to the environment. toxic free and safe.

Description of drawings

Figure 1a shows that the volatile (Z)-3-hexenol pre-treated tea seedlings and then drought-treated plant phenotype and leaf water content; 1b shows the plant phenotype and leaf water content after overexpressing CsADH gene in tobacco and then drought stress water volume.

Figure 2a shows the synthesis pathway of (Z)-3-hexenol; 2b shows the schematic diagram of the gene silencing method; 2c, e show the content of (Z)-3-hexenol in leaves after CsADH gene silencing; 2d shows the relative CsADH after gene silencing Expression level; 2f indicates the phenotype of the drought-treated plants after CsADH gene silencing; 2g, h indicates the leaf angle and leaf water content of the drought-treated plants after CsADH gene silencing.

Detailed ways

In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

Example 1

Regulation of (Z)-3-hexenol and its application in drought resistance of tea plants

1) Add (Z)-3-hexenol with a mother liquor concentration of 1mM on the absorbent cotton, place the absorbent cotton and tea seedlings in a 5L airtight container for 12h, and the aroma will naturally volatilize (final concentration 0.1μg/L, material quality /air volume), treated once every 6 days, and the same amount of solvent was added to the control group, followed by 12 days of drought treatment.

2) Overexpressing the CsADH gene in tobacco can increase the content of (Z)-3-hexenol in tobacco and improve the drought resistance of tobacco plants; silence the CsADH gene in tea trees to reduce the content of (Z)-3-hexenol in tea trees, Reduce the drought resistance of tea trees.

The results show that:

1) Adding (Z)-3-hexenol treatment can significantly improve the drought resistance of tea seedlings. After 12 days of drought, the water content of leaves in the treatment group was significantly higher than that in the control group (Figure 1a).

2) After overexpressing CsADH in tobacco, the drought resistance ability was also significantly improved, and the tobacco leaves showed higher water content (Fig. 1b).

3) CsADH was silenced in tea plants to reduce the content of (Z)-3-hexenol. The results showed that the drought resistance ability of tea seedlings silenced by CsAHD was significantly reduced. The drought resistance ability of (Z)-3-hexenol was further proved (Fig. 2).

In summary, (Z)-3-hexenol is a natural product that can be degraded in nature and is non-toxic and harmless to the environment. Regulating its synthesis has a very high application potential in regulating the drought resistance of tea trees.

Claims (8)

1. Application of (Z)-3-hexenol in drought resistance of tea trees. 2. The application of claim 1, wherein: The working concentration of (Z)-3-hexenol is 0.01-0.5 μg/L. 3. The application according to claim 1 or 2, comprising: Add (Z)-3-hexenol with a working concentration of 0.01-0.5 μg/L to the tea seedlings for slow release, treat for 12 hours, and then carry out drought treatment to improve the drought resistance of the tea seedlings. 4. A kind of overexpression can improve the gene of (Z)-3-hexenol content in tobacco, wherein: The gene is CsADH gene. 5. The gene of claim 4, wherein: Overexpress the CsADH gene, increase the content of (Z)-3-hexenol, and improve the drought resistance of plants. 6. A gene whose silent expression can reduce the content of (Z)-3-hexenol in tea tree, wherein: The gene is CsADH gene. 7. An application of the gene according to claim 6 in constructing low (Z)-3-hexenol content tea plant strains. 8. The use according to claim 7, comprising: Silence the CsADH gene in the tea tree, reduce the content of (Z)-3-hexenol in the tea tree, and reduce the drought resistance of the tea tree.

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