CN116211933B - Anticoccidial traditional Chinese medicine composition and application thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition for resisting coccidiosis and application thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-20 parts of sweet wormwood herb, 10-20 parts of ash bark, 10-14 parts of fructus quisqualis, 8-12 parts of chinaberry bark, 5-10 parts of Hypericum perforatum, 4-8 parts of fried sanguisorba officinalis, 4-8 parts of common andrographis herb, 8-12 parts of astragalus mongholicus and 5-10 parts of prepared rehmannia root. The invention also provides application of the traditional Chinese medicine composition in preparation of anti-coccidiosis medicines. In the traditional Chinese medicine composition provided by the invention, the combination of the sweet wormwood, the ash bark, the rangooncreeper fruit and the chinaberry bark mainly plays roles of killing and expelling insects; hypericum perforatum, fried sanguisorba officinalis and herba andrographitis, cooling blood, relieving swelling, astringing, stopping bleeding, and promoting diuresis; astragalus root and prepared rehmannia root, radix astragali and prepared rehmannia root, radix rehmanniae Preparata, having the effects of invigorating spleen and replenishing qi and enhancing immunity. The traditional Chinese medicine composition provided by the invention mainly takes expelling parasites and detoxifying as main components, takes dysentery stopping and hemostasis as auxiliary components, and takes middle-jiao tonifying and qi tonifying as auxiliary components, and all traditional Chinese medicines are mutually cooperated, so that the anticoccidial effect of the traditional Chinese medicine composition is exerted.

Description

A kind of traditional Chinese medicine composition against coccidiosis and its application

Technical field

The present invention relates to the technical field of traditional Chinese medicine, and in particular to an anti-coccidiosis traditional Chinese medicine composition and its application.

Background technique

Chicken coccidiosis is an important parasitic disease caused by one or more species of Eimeria. It mainly parasitizes the intestinal mucosal epithelial cells of chickens. Its main clinical manifestations are severe diarrhea and excretion. There is a lot of blood in the feces, and some sick chickens will also show obvious neurological symptoms, including coma and being unable to stand normally, and die 2 to 3 days after the symptoms appear. Chicken coccidiosis is characterized by hemorrhagic enteritis, bloody diarrhea, high incidence of chicks, growth retardation, low feed conversion rate and high mortality. It will significantly reduce the production performance of chickens and cause huge economic losses to the poultry industry. Chicken coccidiosis has the characteristics of acute onset, high incidence rate, high fatality rate and great harm.

The development of coccidia gallinae includes three reproductive stages: sporogeny, schizogony and gametogenesis. The development of coccidia is divided into in vivo development and in vitro development. The in vivo development process is more complicated than the in vitro development. After the infectious oocyst is swallowed by the chicken, the oocyst is broken by the mechanical action of the stomach to release the sporangia. It is then excysted in the small intestine due to the action of bile and trypsin. The sporozoites are released and then enter the small intestinal epithelial cells to complete the cleavage. Reproduction and gametogenesis, and finally the egg sac is formed and excreted from the body. The oocysts will form infectious oocysts again after completing spore reproduction under suitable external conditions such as humidity, oxygen and temperature, and then start the next round of infection.

At present, there are methods to prevent and control coccidiosis at home and abroad, such as chemical drug control, adding antibiotics, and coccidiosis vaccine immunization. However, long-term use of chemical drugs and antibiotics will lead to the development of drug resistance and drug residues in poultry products. Not only can it not effectively control chicken coccidia, Insect diseases can also cause harm to the human body. The coccidiosis vaccine has not been well promoted due to problems in its development and application. Chinese herbal medicines have incomparable advantages such as low drug resistance, low drug residues, improved immunity, and promotion of animal growth. Therefore, it is necessary to develop a Chinese herbal medicine preparation to replace traditional anti-coccidial drugs to prevent and treat coccidiosis in chicken production. It is very necessary to prevent insect damage.

Contents of the invention

The purpose of the present invention is to provide an anti-coccidiosis traditional Chinese medicine composition and its application to solve the problems existing in the above-mentioned prior art. The traditional Chinese medicine composition of the present invention has good anti-coccidiosis effect.

In order to achieve the above objects, the present invention provides the following solutions:

The invention provides a traditional Chinese medicine composition for resisting coccidiosis. The traditional Chinese medicine composition comprises the following components by weight: 10-20 parts of Artemisia annua, 10-20 parts of Qinpi, 10-14 parts of Quisqualis quinata, 8-12 parts of Chinaberry bark, 5-10 parts of Hypericum perforatum, 4-8 parts of Sanguisorba officinalis, 4-8 parts of Andrographis paniculata, 8-12 parts of Astragalus and 5-10 parts of Rehmannia glutinosa.

Preferably, the traditional Chinese medicine composition includes the following components in parts by weight: 15 parts of Artemisia annua, 15 parts of Qinpi, 12 parts of Junzi, 10 parts of Melia azedarach, 8 parts of Hypericum perforatum, 6 parts of Fried Sanguisorba, and 6 parts of Andrographis paniculata. 10 parts of Astragalus and 5 parts of Rehmannia glutinosa.

The present invention also provides application of the above-mentioned traditional Chinese medicine composition in preparing anticoccidial drugs.

Further, the coccidiosis is chicken coccidiosis.

The present invention also provides an anticoccidial drug, the raw materials of which include the above-mentioned traditional Chinese medicine composition.

Furthermore, the medicine also includes pharmaceutically acceptable excipients.

Further, the dosage form of the drug includes decoction, ointment, granule, tablet or pill.

The present invention also provides a method for preparing the above-mentioned medicine, which includes the steps of extracting active ingredients from the above-mentioned traditional Chinese medicine composition using a solvent to prepare the medicine.

Further, the solvent includes water and/or ethyl acetate.

Preferably, the solvent is water and ethyl acetate. Artemisia annua, Qinpi, Zhijunzi and Melia azadirachta in the traditional Chinese medicine composition are extracted with ethyl acetate. Hypericum perforatum and Sanguisorba Sanguifolia are extracted in the traditional Chinese medicine composition. , Andrographis paniculata, Astragalus membranaceus and Rehmannia glutinosa active ingredients are extracted using water.

Artemisia annua: It tastes bitter and pungent and is cold in nature. "Shennong's Herbal Classic" records that Artemisia annua has the effects of relieving deficiency fever, cooling blood, relieving heat and treating malaria. It also has antiviral, anti-malarial protozoan, choleretic, antipyretic, analgesic, anti-inflammatory, anti-tumor, antihypertensive and antiarrhythmic effects.

Qinpi: It is the dry branch bark or dry bark of the Oleaceae plant, Ash tree, Ash tree, Ash tree, or Ash tree. Qinpi has the effects of clearing away heat and drying dampness, reducing astringency and stopping dysentery, stopping vaginal discharge, and improving eyesight. It is used for diarrhea caused by damp-heat, red and leucorrhea, red and swollen eyes, and filmy eyes.

Quisqualis indica: The dried and mature fruits of Quisqualis indica L. are used to treat ascariasis, pinworms, abdominal pain caused by worms, and malnutrition in children.

Neem bark: bitter in taste, cold in nature, returns to the liver, spleen, and stomach meridians, has insecticidal and ringworm properties, and is used to treat ascariasis, pinworms, and abdominal pain due to worm infestation.

Hypericum perforatum: It is the whole plant of Hypericum perforatum L. of the Garcinaceae family. It has the effects of astringing and stopping bleeding, regulating menstruation and promoting lactation, clearing away heat and detoxifying, and diuresis.

Fried Sanguisorba officinalis: It is cold in nature and has the effects of clearing heat and detoxifying, cooling blood and stopping bleeding, reducing swelling and relieving pain, and antibacterial.

Andrographis paniculata: It is the dry aerial part of Andrographis paniculata, a plant belonging to the Acanthaceae family. It has the effects of clearing away heat, detoxifying, cooling blood, reducing swelling, and removing dampness.

Astragalus: It has the effects of nourishing qi and raising yang, fixing the surface and stopping sweating, diuresis and swelling, promoting body fluid and nourishing blood, relieving stagnation and relieving paralysis, supporting toxins and draining pus, astringing sores and promoting muscle growth.

Rehmannia glutinosa: It has the effect of nourishing blood and yin, replenishing essence and replenishing marrow.

The invention discloses the following technical effects:

In the traditional Chinese medicine composition provided by the invention, the combination of Artemisia annua, Qinpi, Shijunzi and Melia indica mainly plays an insecticidal and anthelmintic effect, among which Artemisia annua has a certain killing effect on coccidia; the alkaloids in Qinpi can inhibit coccidiosis. The sporulation of insect oocysts reduces the infectivity of oocysts; Junzi and neem bark can promote the discharge of insect bodies and oocysts. Hypericum perforatum, fried Saniyu and Andrographis paniculata can cool blood, reduce swelling, astringe and stop bleeding, and relieve dampness. Astragalus and Rehmannia glutinosa nourish the body and strengthen immunity. The traditional Chinese medicine composition provided by the invention is mainly anthelmintic and detoxifying, supplemented by stopping dysentery and bleeding, and tonifying the body and replenishing qi. The traditional Chinese medicines synergize with each other to exert their anti-coccidial effect.

Detailed ways

Various exemplary embodiments of the invention will now be described in detail. This detailed description should not be construed as limitations of the invention, but rather as a more detailed description of certain aspects, features and embodiments of the invention.

It should be understood that the terms used in the present invention are only used to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges in the present invention, it should be understood that every intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or value intermediate within a stated range, and any other stated value or value intermediate within a stated range, is also included within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range.

Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe methods and/or materials related to the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

It will be apparent to those skilled in the art that various modifications and changes can be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to the skilled person from the description of the invention. The specification and examples of the present invention are exemplary only.

The words "includes", "includes", "has", "contains", etc. used in this article are all open terms, which mean including but not limited to.

Example 1

Ingredients: 150g of Artemisia annua, 150g of Qinpi, 120g of Junzizi, 100g of Neem bark, 80g of Hypericum perforatum, 60g of Fried Sanguisorba, 60g of Andrographis paniculata, 100g of Astragalus membranaceus and 50g of Rehmannia glutinosa.

Preparation:

(1) Weigh each traditional Chinese medicine raw material according to the above raw material formula, mix and crush (less than 20 mesh), then add 2610mL of water, then boil over high heat, simmer over low heat for 2 hours and then filter to obtain the first filtrate and residue;

(2) Add 1305 mL of water to the residue obtained in step (1), boil over high heat, simmer over low heat for 1 h, and then filter to obtain a second filtrate;

(3) Combine the first filtrate and the second filtrate, and then concentrate until the solid content is 60wt% to obtain Chinese medicine ointment A.

Example 2

Ingredients: 200g Artemisia annua, 100g Qinpi, 100g Zhijunzi, 120g Neem bark, 50g Hypericum perforatum, 80g Fried Sanguisorba, 80g Andrographis paniculata, 80g Astragalus membranaceus and 100g Rehmannia glutinosa.

Preparation:

(1) Weigh each traditional Chinese medicine raw material according to the above raw material formula, mix and crush (less than 20 mesh), then add 2730mL of water, then boil over high heat, simmer over low heat for 2 hours and then filter to obtain the first filtrate and residue;

(2) Add 1365 mL of water to the residue obtained in step (1), boil over high heat, simmer over low heat for 1 hour and then filter to obtain the second filtrate;

(3) Combine the first filtrate and the second filtrate, and then concentrate until the solid content is 60wt% to obtain Chinese medicine ointment B.

Example 3

Ingredients: 100g of Artemisia annua, 200g of Qinpi, 140g of Junzizi, 80g of Neem bark, 100g of Hypericum perforatum, 40g of Fried Sanguisorba, 40g of Andrographis paniculata, 120g of Astragalus membranaceus and 80g of Rehmannia glutinosa.

Preparation:

(1) According to the above raw material formula, each Chinese medicinal material is weighed, mixed and crushed (less than 20 meshes), then 2700 mL of water is added, and then boiled over high heat, simmered over low heat for 2 hours, and then filtered to obtain a first filtrate and a residue;

(2) Add 1350 mL of water to the residue obtained in step (1), boil over high heat, simmer over low heat for 1 h, and then filter to obtain a second filtrate;

(3) Combine the first filtrate and the second filtrate, and then concentrate until the solid content is 60 wt% to obtain Chinese medicine ointment C.

Example 4

Same as Example 1, the only difference is that the preparation method is as follows:

(1) Weigh Artemisia annua, Qinpi, Zhijunzi and Neemia bark according to the raw material formula, mix and crush (less than 20 mesh), then add 1560mL ethyl acetate, stir and extract at 60°C for 1 hour, and then filter to obtain the filtrate;

(2) The solvent is evaporated to dryness in the filtrate under reduced pressure, water is added to the evaporated substance, and after uniform mixing, a traditional Chinese medicinal ointment D1 with a solid content of 60wt% is obtained;

(3) Weighing the remaining Chinese medicinal materials according to the raw material formula, and preparing a Chinese medicinal ointment D2 with a solid content of 60 wt % according to the method of Example 1;

(4) Combine D1 and D2 to obtain Chinese medicinal ointment D.

Comparative example 1

The same as Example 1, except that Artemisia annua was replaced by Changshan, and a traditional Chinese medicine ointment E was prepared.

Comparative Example 2

It is the same as Example 1, except that the Chinese medicine ointment F is prepared by replacing the neem bark with the neem bark.

Effect verification

The following takes Example 1 and Example 4 as examples to illustrate the anti-coccidial effect of the traditional Chinese medicine composition of the present invention:

1.Method

1.1 Experimental design

The experimental chickens were 600 healthy Lingshan chicken chicks provided by a breeding farm in Yulin City, and were randomly divided into 6 groups, with 100 chickens in each group. The chickens in 5 groups were infected with 1×10 ⁵ Eimeriatenella oocysts through the cysts using a gavage needle at 15 days of age. The 6th group was not infected and was used as a negative control. From the 1st to the 7th day of infection, each group was administered medication by mixing the drug into the feed as shown in Table 1.

Table 1

Group Whether infected with oocysts drug Dosage negative control group no distilled water 50 mg/kg/day positive control group yes distilled water 50mg/kg/d Drug trial group 1 yes Chinese medicine ointment A 50mg/kg/d Drug trial group 2 yes Traditional Chinese Medicine Ointment D 50mg/kg/d Drug control group 1 yes Chinese medicine ointment E 50mg/kg/d Drug control group 2 yes Chinese medicine ointment F 50 mg/kg/day

1.2 Criteria for judging drug efficacy

(1) Relative weight gain

The animals were weighed on an empty stomach one by one in the morning on the 0th and 8th day after infection, and the relative weight gain rate of each group was recorded and calculated.

Weight gain rate = (M ₈ -M ₀ )m ₀ ×100%, where M ₀ is the weight weighed on an empty stomach on the morning of the 0th day after infection, and M ₈ is the weight weighed on an empty stomach on the morning of the 8th day after infection.

Relative weight gain rate = weight gain rate of the drug test group or positive control drug group/weight gain rate of the negative control group × 100%.

(2)Survival rate

Survival rate (%) = (number of surviving chickens in the group/total number of chickens in the group) × 100%.

(3)Lesion value

On the eighth day of the experiment, the chickens in each group were weighed and killed, and the cecum was removed. The cecal lesions were scored according to the following scoring criteria. If the cecal lesions on both sides were inconsistent, the more serious side was used as the standard. The scoring criteria were:

+0 points: no macroscopic lesions;

+1 point: There are a few scattered congestion spots on the cecal wall, the intestinal wall is not thickened, and the contents are normal;

+2 points: a large number of lesions, obvious blood in the cecal contents, slight thickening of the cecal wall, and normal contents;

+3 points: There is a lot of blood and cecal core (blood clots or grayish white cheesy banana-shaped masses) in the cecum, the cecal wall is obviously thickened, and the feces content in the cecum is small;

+4 points: The cecum is filled with a large amount of blood and intestinal core and the cecum is enlarged. The intestinal core contains or does not contain fecal residue. Dead chickens are also scored with 4 points.

Lesion value = Lesion score × 10.

(4) Oocyst value of cecal contents

Scrape the contents of the cecum and calculate the number of oocysts in grams according to McMaster's method, calculate the oocyst ratio, and convert it into oocyst value according to Table 2.

Oocyst ratio = (number of oocysts in the drug test group or negative control group/number of oocysts in the positive control group) × 100%.

Table 2 Conversion between oocyst ratio and oocyst value

Oocyst ratio 0-1% 1-25% 26-50% 51-75% 76-100% Oocyst value 0 5 10 20 40

(5) Anti-coccidial index (ACI)

ACI = (relative weight gain rate + survival rate) - (lesion value + oocyst value).

Judgment of efficacy: When ACI is less than 120, it is judged to be poor; when ACI is 120-160, it is judged to be moderate; when ACI is 160-180, it is judged to be good; when ACI is greater than 180, it is judged to be excellent. .

2. Results

The survival rate, relative weight gain rate, lesion value and oocyst value of each experimental group were counted, and the anti-coccidial index was calculated. The results are shown in Table 3. At the same time, except for the negative control group, chickens in each group developed severe bloody stools 4-5 days after being infected with coccidia. The bloody stools in the two drug test groups gradually stopped after 6 days. According to Table 3, it can be seen that the traditional Chinese medicine ointments prepared in Example 1 and Example 4 significantly alleviated the impact of coccidial challenge on chicken weight gain; the lesion values and oocyst values of the two drug test groups were significantly lower than those of the positive control group ; From the perspective of anti-coccidial index, the anti-coccidial index of drug test groups 1 and 2 were 162.9 and 180.5 respectively, showing good and excellent anti-coccidial effects respectively. In short, the results show that the Chinese medicinal ointments prepared in Example 1 and Example 4 can effectively treat chicken coccidiosis.

After replacing Artemisia annua and Melia indica in the traditional Chinese medicine composition of the present invention with herbs with similar coccidioidal inhibitory effects, the therapeutic effect is significantly reduced, which shows that Artemisia annua, Qinpi, Shijunzi and Melia indica in the traditional Chinese medicine composition of the present invention There is a certain compatibility relationship and a synergistic effect.

In addition, the present invention takes into account that in the extraction of traditional Chinese medicine, different extraction processes often result in differences in the active ingredients in the extract. In order to achieve efficient extraction of the anti-coccidial active ingredients in the traditional Chinese medicine composition of the present invention, thereby further improving the anti-coccidial Effectively, the present invention uses water with greater polarity and ethyl acetate with less polarity to extract active ingredients. Water extracts mainly hydrophilic components, and ethyl acetate extracts mainly lipophilic components. As a result, It was found that a traditional Chinese medicine ointment prepared by using ethyl acetate as a solvent to extract the active ingredients of Artemisia annua, Qinpi, Shijunzi and Melia azadirachta had a higher anti-coccidial effect. The present invention speculates that in the traditional Chinese medicine composition of the invention, the lipophilic component mainly exerts the anti-coccidial effect.

table 3

The above-described embodiments only describe the preferred modes of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, those of ordinary skill in the art can make various modifications to the technical solutions of the present invention. All deformations and improvements shall fall within the protection scope determined by the claims of the present invention.

Claims (2)

1. A drug with an anthelmintic effect in the body, characterized in that the drug is made from active ingredients extracted from traditional Chinese medicine compositions and pharmaceutically acceptable excipients; In parts by weight, the traditional Chinese medicine composition consists of the following components: 15 parts of Artemisia annua, 15 parts of Qinpi, 12 parts of Junzi, 10 parts of Melia azedarach, 8 parts of Hypericum perforatum, 6 parts of Fried Sanguisorba, 6 parts of Andrographis paniculata, 10 parts of astragalus and 5 parts of rehmannia glutinosa; The extraction method of the active ingredients includes: After weighing Artemisia annua, Qinpi, Zhijunzi and Melia azadirachta, extract with ethyl acetate to obtain an ethyl acetate extract; After weighing Hypericum perforatum, Fried Sanguisorba, Andrographis Paniculata, Astragalus and Rehmannia glutinosa, extract with water to obtain a water extract; The active ingredient is obtained by combining the ethyl acetate extract and the water extract. 2. The medicine according to claim 1, characterized in that the dosage form of the medicine is decoction, ointment, granule, tablet or pill.