CN116422010A - Air pressure and liquid flow rate control device of positive pressure solid phase extraction instrument and use method thereof - Google Patents

Abstract

The invention belongs to the technical field of inspection devices, and in particular relates to a device for controlling air pressure and liquid flow rate of a positive pressure solid-phase extraction instrument and a method for using the same. It includes several extraction columns, and a measuring cylinder is arranged directly below the outlet of the lower end of a single extraction column. A multi-point laser sensor is provided on the common side of the lower end outlet of the measuring cylinder and the extraction column; an adsorbent packing sieve is placed at the bottom of the individual extraction column, and the sample liquid B is above the adsorbent packing sieve, and the sample liquid B The liquid surface to the lower surface of the cover is pressurized air or inert gas. The cover is screwed on the upper port of the extraction column. There is a pressure sensor on the cover. A gas slip ring is installed in the center of the cover. The other end of the air hose is connected to the electric pressure regulating valve, and the air inlet end of the electric pressure regulating valve is connected to the air source. The device solves the problem of controlling the flow rate of the liquid at the outlet of the extraction column, and also prevents the situation that individual extraction columns are blocked and bursts, thereby improving the detection quality and efficiency.

Description

Air pressure and liquid flow rate control device of a positive pressure solid phase extraction instrument and its application method

technical field

The invention belongs to the technical field of inspection devices, and in particular relates to a device for controlling air pressure and liquid flow rate of a positive pressure solid phase extraction instrument and a method for using the same.

Background technique

Solid-phase extraction is a sample pretreatment process in the inspection process. It uses a solid adsorbent to separate the target compound in the liquid sample from the sample matrix and interfering compounds, and then elutes with an eluent or heats to desorb to achieve separation. and the purpose of enriching target compounds. The solid phase extraction device has positive pressure and negative points. The positive pressure solid phase extraction device uses air as the power source to pass through the solid phase extraction column, and the negative pressure solid phase extraction device uses vacuum negative pressure as the power source to pass through the solid phase extraction column. Most of the solid phase extraction columns are syringe-type polypropylene tubes. The lower part of the column is equipped with an adsorbent packing sieve plate. The upper end of the solid phase extraction column is open, and the liquid is discharged from the outlet at the lower end of the extraction column through the adsorbent packing sieve plate. Positive pressure solid phase extraction is that the plug rod is vertically inserted into the extraction column, and the sample liquid is discharged from the outlet of the lower end of the extraction column through the adsorbent sieve through the positive pressure of the air. It is also possible to seal the upper end of the extraction column with a cover and pass through pressure air or inert gas. The sample liquid is discharged from the outlet of the lower end of the extraction column through the adsorbent sieve plate. The speed of the liquid flowing out from the outlet is strictly required. If the flow rate does not meet the requirements, the filtration of impurities will not be complete and the determination of the target content will be affected. Although the extraction instrument can Setting the pressure of air or inert gas entering the sealing cap cannot ensure the flow rate of the outlet liquid. The reason is that the reaction of each individual sample liquid passing through the sieve plate of the adsorbent packing is different, and the pressure of the gas entering the extraction column cavity is different. They are all basically the same, which causes the droplet flow rate at the lower end of some extraction columns to be fast or slow, and the liquid flow rate at the outlet of each extraction column cannot be effectively controlled. When the sieve plate is blocked, the unsafe situation that the pressure in the inner cavity of the extraction column is too high and the cover bursts occurs, which leads to a decline in the quality and efficiency of the test.

purpose of invention

In order to make up for the deficiencies in the existing technology, a device for controlling the air pressure and liquid flow rate of a positive pressure solid phase extraction instrument and its use method are provided to control the flow rate of the liquid at the outlet of each extraction column, and at the same time, it will not be capped when "blockage" occurs Burst, to achieve the purpose of improving detection quality and efficiency.

Contents of the invention

The present invention is achieved through the following technical solutions:

An air pressure and liquid flow rate control device for a positive pressure solid phase extraction instrument, comprising several extraction columns, a measuring cylinder is arranged directly below the outlet of the lower end of a single extraction column, and multiple Point laser sensor;

An adsorbent packing sieve plate is placed at the bottom of each extraction column described above, the sample liquid B is above the adsorbent packing sieve plate, and the pressure air or inert gas is from the liquid surface of the sample liquid B to the lower surface of the cover, and the cover is screwed on On the upper port of the extraction column, the cover is equipped with a pressure sensor, and the center of the cover is equipped with an air slip ring. The air inlet of the air slip ring is connected to an air hose. The air inlet terminal is connected to the air source.

On the basis of the above scheme, preferably, several extraction columns are vertically placed in the holes provided on the upper plate of the support and the lower plate of the support, wherein the holes on the upper plate of the support are positioned radially to the upper part of the extraction column, and the holes on the lower plate of the support are aligned The lower part of the extraction column is positioned radially and axially.

On the basis of the above solution, preferably: the electric control wires of the multi-point laser sensor, the pressure sensor, and the electric pressure regulating valve are all wired or wirelessly connected to the display PLC controller.

The method for using the air pressure and liquid flow rate control device of the positive pressure solid phase extraction instrument comprises the following steps:

S1. Put the adsorbent packing sieve plate into each extraction column, inject the sample liquid B and tighten the cap, insert vertically into the holes set on the upper plate of the support and the lower plate of the support, press the work button in the display PLC controller, All electronic control components enter the working state;

S2. The PLC controller controls the electric pressure regulating valve to open slowly to the programmed setting range, and each multi-point laser sensor will monitor the changes in the speed of droplet falling and the liquid level of liquid A in the measuring cylinder and the position of the measurement scale line, and the image data will be real-time Send to PLC controller;

S3. When the PLC controller judges according to the programming that the image and monitoring data of the droplet falling at the outlet of the lower end of an extraction column are about to exceed the upper limit allowed by the programming, the PLC controller controls the electric pressure regulating valve connected to the extraction column to properly depressurize and supply gas;

S4. When the PLC controller judges according to the programming that the image and monitoring data of the droplet falling at the outlet of the lower end of the extraction column are about to fall below the allowable lower limit of the programming, the PLC controller controls the electric pressure regulating valve connected to the extraction column to properly pressurize the gas supply; through Steps S3 and S4 control the flow rate of the outlet droplets at the lower end of each extraction column to always comply with the programmed setting;

S5. When the PLC controller judges according to the programming that the image and monitoring data of the droplet falling at the outlet of the lower end of an extraction column are about to fall below the allowable lower limit of the programming, the PLC controller controls the electric pressure regulating valve connected to the extraction column to properly pressurize the gas supply, but The image and monitoring data of the droplet drop at the outlet of the extraction column have not improved significantly, and when the pressure value of the pressure sensor on the cover of the extraction column is about to reach the specified limit, the PLC controller controls the electric pressure regulating valve connected to the extraction column Stopping the gas supply indicates that a clogging state occurs between the sample liquid B in the extraction column and the adsorbent packing sieve plate 8 .

Beneficial technical effect of the present invention:

In the present invention, the same pressure gas is delivered to all the extraction columns in the original extraction instrument at the same time, and the air pressure in the individual extraction columns cannot be adjusted independently, but instead, the air pressure in each individual extraction column can be controlled independently. The original extraction instrument cannot effectively control the flow rate of the outlet liquid, but it can be used to quantitatively control the outlet flow rate (flow rate) to eliminate the bursting of the extraction column cover.

The air pressure and liquid flow rate control device of the positive pressure solid phase extraction apparatus of the present invention solves the problem of controlling the liquid flow rate at the outlet of the extraction column, and also prevents individual extraction columns from being blocked and burst, thereby improving the detection quality and efficiency.

Description of drawings

Structural features and methods of use of the present invention are further described in conjunction with the accompanying drawings:

Fig. 1 is a main sectional view of the present invention;

Fig. 2 is a control schematic diagram;

In the figure: 1. Multi-point laser sensor, 2. Liquid A, 3. Graduated cylinder, 4. Workbench, 5. Display PLC controller, 6. Adsorbent packing sieve plate, 7. Sample liquid B, 8. Extraction column, 9, cap, 10, air slip ring, 11, air hose, 12, electric pressure regulating valve, 13, pressure sensor, 14, support upper plate, 15, support lower plate.

Detailed ways

It should be noted that the following detailed description is exemplary and intended to provide further explanation of the present invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

It should be noted that the terminology used here is only for describing specific embodiments, and is not intended to limit exemplary embodiments according to the present invention. As used herein, unless the invention clearly states otherwise, the singular form is also intended to include the plural form. In addition, it should also be understood that when the terms "comprising" and/or "comprising" are used in this specification, their Indicate the presence of features, steps, operations, means, components and/or combinations thereof;

For the convenience of description, if the words "up", "down", "left" and "right" appear in the present invention, it only means that they are consistent with the directions of up, down, left and right in the drawings themselves, and do not limit the structure. It is for the convenience of describing the present invention and simplifying the description, but does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.

Explanation of terms: The terms "installation", "connection", "connection", "fixation" and other terms in the present invention should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral body; It can be a mechanical connection, or an electrical connection, a direct connection, or an indirect connection through an intermediary, or an internal connection between two components, or an interaction relationship between two components. For those of ordinary skill in the art, , the specific meanings of the above terms in the present invention can be understood according to specific situations.

Fig. 1-2 is one of embodiment of the present invention.

An air pressure and liquid flow rate control device for a positive pressure solid phase extraction instrument is mainly composed of: an electric pressure regulating valve 12, a pressure sensor 13, an electric pressure regulating valve 12, a multi-point laser sensor 1, and a display PLC controller 5;

In the present embodiment, a plurality of extraction columns 8 are placed vertically in the holes provided by the upper plate 14 of the support and the lower plate 15 of the support, wherein the holes of the upper plate 14 of the support are radially positioned on the upper part of the extraction column 8, and the holes of the lower plate 15 of the support are positioned vertically. For the radial and axial positioning of the lower part of the extraction column 8, a measuring cylinder 3 is provided directly below the outlet of the lower end of the extraction column 8, and the measuring cylinder 3 is horizontally placed on the workbench table 4, and the measuring cylinder 3 is provided with a measuring scale line, between the measuring cylinder 3 and The common side of the exit is provided with a multi-point laser sensor 1. The multi-point laser sensor 1 is placed on the workbench 4 through a tripod. The function and function of the multi-point laser sensor 1 is to confirm the position of the target through a high-definition camera. Laser tracking obtains multi-point change information. That is, the monitoring of the change of the falling speed of the liquid drop and the liquid level of the liquid A2 in the measuring cylinder 3 and the position of the measuring scale line are realized. An adsorbent packing sieve plate 6 is placed at the inner bottom of the extraction column 8, and the top of the adsorbent packing sieve plate 6 is the sample liquid B7, and the liquid surface of the sample liquid B7 to the lower surface of the cover 9 is pressurized air or inert gas, and the cover 9 is screwed On the upper port of the extraction column 8, a pressure sensor 13 is provided on the cover 9, and an air slip ring 10 is installed in the center of the cover 9. The pressure regulating valve 12, the air inlet end of the electric pressure regulating valve 12 is connected to the gas source.

Connect the electric control wires of each multi-point laser sensor 1, pressure sensor 13, and electric pressure regulating valve 12 to the display PLC controller 5 by wire or wirelessly, and input the control program, image analysis and self-learning adaptive program to the PLC controller If the adjustment range of the electric pressure regulating valve 12 corresponding to the normal droplet falling frequency range is set, the electric pressure regulating valve 12 will no longer supply air to the extraction column 8 when the pressure sensor 13 reaches the specified upper limit.

How to use: Put each extraction column 8 into the sieve plate 6 filled with adsorbent, inject the sample liquid B7 and tighten the cover 9, insert vertically into the holes set on the upper plate 14 of the bracket and the lower plate 15 of the bracket, press to display the PLC control Press the working button in the device 5, and each electric control part enters the working state. The PLC controller controls the electric pressure regulating valve 12 to slowly open to the programmed setting range, and each multi-point laser sensor 1 will monitor the change of the falling speed of the liquid drop and the liquid level of the liquid in the measuring cylinder 3 and the position of the measurement scale line. The image data is real-time Sent to the PLC controller, when the PLC controller judges according to the programming that the image and monitoring data of the droplet falling at the outlet of a certain extraction column 8 is about to exceed the upper limit of the programming, the PLC controller controls the electric pressure regulating valve 12 connected to the extraction column 8 Appropriately depressurize the air supply, and vice versa pressurize the air supply. This realizes the effective control of the flow rate of the droplet at the outlet of the lower end of each extraction column 8 and always conforms to the programmed setting. When the PLC controller judges according to the programming that the image and monitoring data of the droplet falling at the outlet of the lower end of an extraction column 8 is about to fall below the allowable lower limit of the programming, the PLC controller controls the electric pressure regulating valve 12 connected to the extraction column 8 to properly pressurize and supply air. However, the images and monitoring data of the droplets falling at the outlet of the lower end of the extraction column 8 are still not significantly improved, and when the pressure value of the pressure sensor 13 on the cover 9 of the extraction column 8 is about to reach the specified limit, the PLC controller controls the connection of the extraction column. The electric pressure regulating valve 12 of 8 stops supplying gas, and this also shows that the sample liquid B7 in this extraction column 8 and the sorbent packing sieve plate 6 have clogged state and see accompanying drawing 1, because no longer to this extraction column 8 The internal gas supply prevents the extraction column 8 cover from bursting. The droplet at the outlet of the lower end of each extraction column 8 and the change of the liquid level of the liquid A2 in the measuring cylinder 3 and the measurement scale line can be traced in the display PLC controller 5 for the entire image measurement data.

The device solves the problem of controlling the flow rate of the liquid at the outlet of the extraction column 8, and also prevents the situation that individual extraction columns 8 are blocked and bursts, thereby improving the detection quality and efficiency.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (4)

1. The utility model provides a pneumatic pressure and liquid velocity of flow controlling means of malleation solid phase extraction appearance which characterized in that:

the multi-point laser sensor comprises a plurality of extraction columns (8), wherein a measuring cylinder (3) is arranged right below the lower end outlets of the extraction columns (8), and a multi-point laser sensor (1) is arranged on the side surface of the measuring cylinder (3) which is common to the lower end outlets of the extraction columns (8);

the single extraction column (8) in the bottom place adsorbent filler sieve (6), adsorbent filler sieve (6) top is sample liquid B (7), sample liquid B (7) liquid level is pressure air or inert gas to closing cap (9) lower surface, closing cap (9) are fixed soon in extraction column (8) upper shed, be equipped with pressure sensor (13) on closing cap (9), closing cap (9) center is equipped with gas sliding ring (10), gas sliding ring (10) air inlet air hose (11), electric pressure regulating valve (12) are connected to the other end of gas hose (11), electric pressure regulating valve (12)'s air inlet termination air supply.

2. The positive pressure solid phase extractor air pressure and liquid flow rate control device according to claim 1, wherein: the extraction columns (8) are vertically placed in holes formed in the upper support plate (14) and the lower support plate (15), wherein the holes of the upper support plate (14) are used for radially positioning the upper part of the extraction columns (8), and the holes of the lower support plate (15) are used for radially and axially positioning the lower part of the extraction columns (8).

3. The positive pressure solid phase extractor air pressure and liquid flow rate control device according to claim 2, wherein: the electric control wires of the multipoint laser sensor (1), the pressure sensor (13) and the electric pressure regulating valve (12) are connected with the display PLC (5) in a wired or wireless mode.

4. A method of using the positive pressure solid phase extractor air pressure and liquid flow rate control device of claim 3, wherein: the method comprises the following steps:

s1, pouring the sample liquid B (7) into the adsorbent filling sieve plate (6) placed in each extraction column (8), screwing the sealing cover (9), vertically inserting the sample liquid B into holes formed in the support upper plate (14) and the support lower plate (15), pressing and displaying a working button in the PLC (5), and enabling each electric control part to enter a working state;

s2, the PLC controller controls the electric pressure regulating valve (12) to be slowly opened to a programming setting range, and each multipoint laser sensor (1) sends image data of the falling speed change condition of each monitoring liquid drop and the liquid level of the liquid A (2) in the measuring cylinder (3) and the position condition of the measuring scale mark to the PLC controller in real time;

s3, when the PLC judges that the image and the monitoring data of the falling of the outlet liquid drop at the lower end of a certain extraction column (8) are about to be higher than the upper limit of the programming permission according to the programming, the PLC controls an electric pressure regulating valve (12) connected with the extraction column (8) to properly decompress and supply air;

s4, when the PLC judges that the image and the monitoring data of the falling of the outlet liquid drop at the lower end of a certain extraction column (8) are about to be lower than the lower limit of the programmed permission according to programming, the PLC controls an electric pressure regulating valve (12) connected with the extraction column (8) to appropriately boost and supply air; s3 and S4 are used for controlling the flow rate of the outlet liquid drops at the lower end of each extraction column (8) to always accord with the programming setting;

s5, when the PLC controller judges that the image and the monitoring data of the falling of the outlet liquid drop at the lower end of a certain extraction column (8) are about to be lower than the lower limit of the programmed permission according to the programming, the PLC controller controls the electric pressure regulating valve (12) connected with the extraction column (8) to properly boost and supply air, but the image and the monitoring data of the falling of the outlet liquid drop at the lower end of the extraction column (8) are not obviously improved, and when the pressure value of the pressure sensor (13) on the sealing cover (9) of the extraction column (8) is about to reach the specified limit value, the PLC controller controls the electric pressure regulating valve (12) connected with the extraction column (8) to stop supplying air, so that a blocking state appears between the sample liquid B (7) in the extraction column (8) and the adsorbent filling sieve plate (6).

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