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CN117064805B - Anti-aging composition and preparation method and application thereof - Google Patents

Anti-aging composition and preparation method and application thereof Download PDF

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Publication number
CN117064805B
CN117064805B CN202310865393.1A CN202310865393A CN117064805B CN 117064805 B CN117064805 B CN 117064805B CN 202310865393 A CN202310865393 A CN 202310865393A CN 117064805 B CN117064805 B CN 117064805B
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extract
aging
preparation
aging composition
valerian root
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CN117064805A (en
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张芳
杨隆河
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Third Institute of Oceanography MNR
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Third Institute of Oceanography MNR
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses an anti-aging composition, a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition comprises a kulang extract and a valerian root extract. The invention can synergistically increase the cell activity and reduce the expression of cell senescence genes by compounding the amaranthus extract and the valerian root extract, and has remarkable anti-aging effect. The composition is a pure plant extract, has good safety, simple preparation method and low cost, and is suitable for industrialized popularization.

Description

Anti-aging composition and preparation method and application thereof
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging composition, a preparation method and application thereof.
Background
In recent years, components capable of delaying skin aging are found from some natural green plants, and the components have the characteristics of safety, high efficiency and the like, so that the components are widely applied to cosmetics by researchers.
CN110731928a discloses an anti-aging cosmetic composition, which discloses that birch juice with concentration multiple of 1.05-8 times has anti-aging effect. CN109394591a discloses an anti-aging cosmetic process, which uses ethanol as an extraction solvent, uses ultrasonic waves to extract, and the obtained seaweed extract can inhibit the heart returning of elastase and remove free radicals, and has the efficacy of delaying skin aging. CN111643427a discloses an anti-aging composition comprising red ginseng extract, ginseng root water, turmeric root extract, cistanche extract, dendrobium stem extract, ganoderma sinensis extract, poria extract, dogwood extract, longleaf clover extract and polygonum cuspidatum root extract. The anti-aging composition has good oxidation resistance and cell activation effects, can prevent skin aging, can effectively improve skin aging condition, and has excellent anti-aging effect.
Today advocating green life, people have a certain rejection psychology on chemically synthesized cosmetic raw materials, and compared with artificially synthesized anti-aging compounds, natural plant extracts meet the consumption concept of most consumers on green and environment-friendly cosmetics. In addition, the cosmetic has good efficacy, so that the cosmetic has been rapidly developed in recent years, and the cosmetic will also occupy an important position in the daily chemical market.
Mangrove plant ku Lang Shu belongs to the genus of dyers woad (Clerodendrum) of the family Verbenaceae, and is mainly distributed in the south of Zhejiang, fujian, guangdong, hainan and Guangxi coast beach in China, and is a tree species for coastal sand fixation forestation in the south of China. The kulang tree has a certain medicinal value, and the ku Lang Shugen can be used as a medicine for clearing heat and detoxicating, relaxing tendons and activating collaterals. As a traditional medicine in thailand, fresh leaves of kulang are used for treating skin diseases, the powder ground into which leaves of kulang are ground is used for treating edema, muscle pain, rheumatalgia, and its roots are also used for treating venereal diseases together with camphor, garlic and pepper.
At present, plant extracts with anti-aging efficacy are more, but research on the anti-aging aspect of the amaranthus martensi karsch extract is not found through searching.
Disclosure of Invention
In order to solve the technical problems, the invention provides an anti-aging composition, a preparation method and application thereof, and the composition can improve the cell activity, reduce the expression of cell aging genes and has obvious anti-aging effect through compounding of a kulang extract and a valerian root extract.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the invention provides an anti-aging composition, which comprises a kulang extract and a valerian root extract.
Preferably, the mass ratio of the kulang extract to the valerian root extract is 3-10:1.
Preferably, the kulang extract is an alcoholic extract of at least one of the root, stem, leaf of ku Lang Shu.
Preferably, the valerian root extract is an alcoholic extract of valerian root.
Further preferably, the preparation method of the kulang extract or valerian root extract comprises the following steps:
(1) Crushing the root of the alocasia or valerian to obtain material powder;
(2) Adding an ethanol water solution into the powder obtained in the step (1), and refluxing to obtain an extracting solution;
(3) And (3) freeze-drying the extract obtained in the step (2) to obtain the amaranthus martensi karsch extract or valerian root extract.
Preferably, the particle size of the powder in step (1) is 60-120 mesh.
Preferably, the feed liquid ratio (g/100 ml) of the powder and the ethanol water solution in the step (2) is 1:10-15.
Preferably, the volume percentage of the ethanol aqueous solution in the step (2) is 60-80%.
Preferably, the reflux in step (2) is at 70-85 ℃ for 1.5-4 hours.
The invention also provides a preparation method of the anti-aging composition. Specifically, the preparation method comprises mixing the extract of Sophora alopecuroides and the extract of valerian root.
The invention also provides application of the anti-aging composition in preparation of cosmetics.
The invention also provides an anti-aging cosmetic which is prepared from the anti-aging composition or the anti-aging composition prepared by the preparation method.
The beneficial effects of the invention are as follows:
(1) The composition of the invention can synergistically increase the cell activity, reduce the expression of cell senescence genes and has obvious anti-aging effect through the compounding of the kulang extract and the valerian root extract.
(1) Tests show that the compound preparation of the amaranthus extract and the valerian root extract can also reduce the expression of the beta-galactosidase of the HSF cells induced by D-galactose and delay the cell aging.
(2) The composition is a pure plant extract, has good safety, simple preparation method and low cost, and is suitable for industrialized popularization.
Drawings
FIG. 1 shows the effect of the examples and comparative examples on hydrogen peroxide-induced cell senescence death.
FIG. 2 shows the effect of examples and comparative examples on the expression of hydrogen peroxide-induced cellular senescence genes.
FIG. 3 shows the effect of an example of the invention on D-galactose induced expression of beta-galactosidase in HSF cells.
Detailed Description
Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes embodiments of the present invention in conjunction with specific embodiments. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention.
Before further describing embodiments of the invention, it is to be understood that the scope of the invention is not limited to the specific embodiments described below, and that the terminology used in the examples of the invention is intended to be in the nature of specific embodiments and is not intended to be limiting of the scope of the invention.
The invention does not limit the sources of the adopted raw materials, and if no special description exists, the adopted raw materials are all common commercial products in the technical field.
EXAMPLE 1 anti-aging composition
An anti-aging composition comprises 3:1 parts by weight of Sophora alopecuroides extract and valerian root extract.
The preparation method of the kulang extract or valerian root extract comprises the following steps:
(1) Crushing ku Lang Shugen or valerian root, and sieving with a 60-mesh sieve to obtain powder;
(2) Adding 80% ethanol water solution into the powder obtained in the step (1) according to a feed-liquid ratio of 1:10, and refluxing for 1.5h at 85 ℃ to obtain an extracting solution;
(3) And (3) spin-drying the extract obtained in the step (2), and freeze-drying to obtain the alocasia extract or valerian root extract.
EXAMPLE 2 anti-aging composition
An anti-aging composition comprises a 10:1 mass ratio of Sophora alopecuroides extract and valerian root extract.
The preparation method of the kulang extract or valerian root extract comprises the following steps:
(1) Crushing ku Lang Shugen or valerian root, and sieving with 120 mesh sieve to obtain powder;
(2) Adding 60% ethanol water solution into the powder obtained in the step (1) according to a feed-liquid ratio of 1:15, and refluxing for 4 hours at 70 ℃ to obtain an extracting solution;
(3) And (3) spin-drying the extract obtained in the step (2), and freeze-drying to obtain the alocasia extract or valerian root extract.
Example 3 anti-aging composition and method of making the same
An antiaging composition comprises 5:1 by mass of Sophora alopecuroides extract and valerian root extract.
The preparation method of the kulang extract or valerian root extract comprises the following steps:
(1) Crushing ku Lang Shugen or valerian root, and sieving with 80 mesh sieve to obtain powder;
(2) Adding 75% ethanol water solution into the powder obtained in the step (1) according to a feed-liquid ratio of 1:12, and refluxing for 2.5 hours at 80 ℃ to obtain an extract;
(3) And (3) spin-drying the extract obtained in the step (2), and freeze-drying to obtain the alocasia extract or valerian root extract.
Example 4 anti-aging composition
This example differs from example 3 only in that the anti-aging composition consists of a 7:1 mass ratio of the kulang extract and valerian root extract.
Example 5 anti-aging composition
This example differs from example 3 only in that the leaves of kulang extract of kulang Lang Shu are used in the preparation process in the anti-aging composition.
EXAMPLE 6 anti-aging composition
This example differs from example 3 only in that the kulang extract in the anti-aging composition employs the stems of kulang during the preparation process.
Comparative example 1 anti-aging composition
This comparative example differs from example 3 only in that the anti-aging composition is a kulang extract.
Comparative example 2 anti-aging composition
This comparative example differs from example 3 only in that the anti-aging composition is valerian root extract.
The anti-aging effect of the invention is evaluated by examining the effect of the anti-aging composition on hydrogen peroxide-induced cell aging.
1. Protection effect on cell aging and death induced by hydrogen peroxide
HaCat cells were cultured in DMEM medium containing 10% fetal bovine serum (dmem+10% fbs+1% p-S) and placed in a 5% carbon dioxide incubator at 37 ℃. Cells were plated into 96-well plates 100 μl per well when the cells were in log phase, cell numbers were adjusted to 1 ten thousand cells/well), 6 parallel wells were set per group, and incubated overnight. A volume of 50. Mu.L of anti-aging composition solution (12.5. Mu.g/mL, DMSO as solvent) was added every well every day, and the control group was added with an equal amount of DMSO solvent. After 1 hour, 400. Mu.M hydrogen peroxide was added to each well and incubation was continued for 4 hours, and 10. Mu.L of CCK-8 reagent (Japan Kogyo) was added thereto, and incubation was continued for 2 hours, and absorbance was measured by a 450nm microplate reader.
Cell viability (%) = (OD composition/OD control) ×100%
The results are shown in FIG. 1. As can be seen from FIG. 1, before molding, there was no significant difference in cell activity (P > 0.05) between each group compared with the control group, after molding, the control group showed a significant difference in cell activity (aa P < 0.01) compared with the control group before molding, indicating that aging and death of cells induced by hydrogen peroxide occurred and the cell activity was significantly reduced.
The cell activities of each of examples 1 to 6 were significantly different (b P < 0.05) compared to the molded control group, wherein the cell activities of examples 3,5 and 6 were significantly different (bb P < 0.01), indicating that the anti-aging composition of the present invention has a protective effect on hydrogen peroxide-induced cell aging, and the effects of examples 3,5 and 6 were superior.
The cell activities of the comparative examples 1 and 2 after molding showed very significant differences (aa P < 0.01) compared to those before molding, and no significant differences were observed in the cell activities compared to the control group after molding. It is shown that at a dose of 12.5 μg/mL, a single Sophora alopecuroides extract or valerian root extract is not protective against hydrogen peroxide induced cell senescence.
2. Reducing the expression of cell senescence genes induced by hydrogen peroxide
After the cells were dosed as above, cellular RNA was extracted using TriZol RNA extraction reagent (Invitrogen, USA) according to the following procedureThe method comprises the following operation steps of the specification of the Super total RNA extraction kit, and total RNA is extracted and purified. RNA concentration was determined using a NanoDrop2000, using total RNA as template, and the total amount of RNA in the system was controlled to be about 1. Mu.g, which was reverse transcribed into cDNA according to REVERTRA ACE QPCR RT MASTER Mix reverse transcription kit instructions. The cDNA template obtained by reverse transcription was diluted 10-fold to 100. Mu.L, and qPCR experiments were performed using the diluted cDNA as a template. Primer sequences of related genes are as follows:
MMP-1 gene expression results are shown in FIG. 2. As shown in FIG. 2, compared with the MMP-1 gene expression amount after the molding of the control group, the MMP-1 gene expression amount has a significant difference (aa P < 0.01), which indicates that the cell induced by hydrogen peroxide can promote the MMP-1 gene expression.
Compared with a control group after molding, the MMP-1 gene expression amounts of the embodiment 1 to the embodiment 6 after molding have extremely remarkable differences (bb P is less than 0.01), which indicates that the anti-aging composition provided by the embodiment 1 to the embodiment 6 can inhibit the MMP-1 gene expression induced by hydrogen peroxide and delay cell aging.
Compared with the molded control group, the MMP-1 gene expression amount of the molded control example 1 has significant difference (b P < 0.05), and the MMP-1 gene expression amount of the molded control example 2 has no significant difference (P > 0.05), which shows that under the dosage of 12.5 mug/mL, a single kulang extract can inhibit the MMP-1 gene expression induced by hydrogen peroxide, and a single valerian root extract can not inhibit the MMP-1 gene expression induced by hydrogen peroxide.
3. Reducing D-galactose induced expression of beta-galactosidase in HSF cells
Human skin fibroblasts, HSF, were cultured in DMEM medium (DMEM+15% FBS+1% P-S) containing 15% fetal bovine serum, and placed in a 5% carbon dioxide incubator at 37 ℃. Normal control, model and extract groups (anti-aging composition prepared in example 2) were placed in 96-well plates. After the model group and the extract group were cultured with a complete culture solution containing 20mg/mL of D-galactose for 24 hours, the extract group was dosed (12.5. Mu.g/mL, the solvent was DMSO), the module group was dosed with an equal amount of DMSO solvent, and then the culture was continued in the complete culture solution for 24 hours.
Staining was performed according to the instructions of the cell senescence beta-galactosidase staining kit (Shanghai Biyun biotechnology Co., ltd.), and observed and photographed under an inverted microscope. The results are shown in FIG. 3. Compared with the model group, the positive number of the beta-galactosidase staining cells of the extract group is reduced after the anti-aging composition is added, which shows that the anti-aging composition can delay cell aging.
It should be emphasized that the examples described herein are illustrative rather than limiting, and therefore the invention includes, but is not limited to, the examples described in the detailed description, as other embodiments derived from the technical solutions of the invention by a person skilled in the art are equally within the scope of the invention.

Claims (5)

1.一种抗衰老的组合物，其特征在于，为苦郎树提取物和缬草根提取物，所述苦郎树提取物和缬草根提取物的质量比为3-10:1；所述苦郎树提取物为苦郎树的根、茎、叶中至少一种的提取物；1. An anti-aging composition, characterized in that it comprises a bitter orange extract and a valerian root extract, wherein the mass ratio of the bitter orange extract to the valerian root extract is 3-10:1; the bitter orange extract is an extract of at least one of the roots, stems and leaves of the bitter orange; 所述苦郎树提取物或缬草根提取物的制备方法，包括以下步骤：The preparation method of the valerian tree extract or the valerian root extract comprises the following steps: （1）将苦郎树或缬草根破碎，得到料粉；(1) Crush the roots of valerian or valerian to obtain powder; （2）向步骤（1）得到的料粉中加入乙醇水溶液，回流，得到提取液；(2) adding ethanol aqueous solution to the powder obtained in step (1), refluxing, and obtaining an extract; （3）将步骤（2）得到的提取液冷冻干燥，得到所述苦郎树提取物或缬草根提取物；(3) freeze-drying the extract obtained in step (2) to obtain the Kulang tree extract or the valerian root extract; 步骤（2）中所述乙醇水溶液的体积百分数为60-80%。The volume percentage of the ethanol aqueous solution in step (2) is 60-80%. 2.根据权利要求1所述的组合物，其特征在于，步骤（2）中所述回流为：70-85℃下回流1.5-4h。2. The composition according to claim 1, characterized in that the reflux in step (2) is: reflux at 70-85°C for 1.5-4h. 3.一种权利要求1-2任一项所述的抗衰老的组合物的制备方法，其特征在于，所述制备方法为：将配方量的苦郎树提取物和缬草根提取物混合即可。3. A method for preparing the anti-aging composition according to any one of claims 1-2, characterized in that the preparation method comprises: mixing the Kulang Tree extract and the Valerian root extract in a prescribed amount. 4.权利要求1-2任一项所述的抗衰老的组合物或权利要求3所述的制备方法制备得到的抗衰老的组合物在制备化妆品中应用。4. Use of the anti-aging composition according to any one of claims 1 to 2 or the anti-aging composition prepared by the preparation method according to claim 3 in the preparation of cosmetics. 5.一种抗衰老的化妆品，由权利要求1-2任一项所述的抗衰老的组合物或权利要求3所述的制备方法制备得到的抗衰老的组合物制成。5. An anti-aging cosmetic, made from the anti-aging composition according to any one of claims 1 to 2 or the anti-aging composition prepared by the preparation method according to claim 3.
CN202310865393.1A 2023-07-14 2023-07-14 Anti-aging composition and preparation method and application thereof Active CN117064805B (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI287997B (en) * 2000-12-15 2007-10-11 Yakult Honsha Kk Compositions for retarding skin aging

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* Cited by examiner, † Cited by third party
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JP2011184308A (en) * 2010-03-04 2011-09-22 Shiseido Co Ltd External preparation for skin
CN102274288B (en) * 2010-06-09 2013-07-17 台湾糖业股份有限公司 Use of angelica extract as a medicament for preventing skin aging
CN107308016A (en) * 2017-07-18 2017-11-03 佛山市聚成生化技术研发有限公司 A kind of anti-oxidant sleep mask

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI287997B (en) * 2000-12-15 2007-10-11 Yakult Honsha Kk Compositions for retarding skin aging

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