CN117825673B - Exosome detection device - Google Patents
Exosome detection device Download PDFInfo
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- CN117825673B CN117825673B CN202410240136.3A CN202410240136A CN117825673B CN 117825673 B CN117825673 B CN 117825673B CN 202410240136 A CN202410240136 A CN 202410240136A CN 117825673 B CN117825673 B CN 117825673B
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- water bath
- exosome
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 63
- 238000001514 detection method Methods 0.000 title claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 183
- 238000010438 heat treatment Methods 0.000 claims abstract description 89
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims description 19
- 238000009413 insulation Methods 0.000 claims description 9
- 230000000670 limiting effect Effects 0.000 claims description 7
- 238000004321 preservation Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 239000012774 insulation material Substances 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 abstract description 8
- 238000009434 installation Methods 0.000 abstract description 6
- 230000007306 turnover Effects 0.000 abstract description 5
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 230000001681 protective effect Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 6
- 239000002699 waste material Substances 0.000 description 6
- 230000009471 action Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Devices For Medical Bathing And Washing (AREA)
Abstract
The invention discloses an exosome detection device which comprises a protection shell, wherein an installation seat is fixedly arranged on the lower side wall of the protection shell, a screw hole is formed in the top of the installation seat in a penetrating mode, an external thread pipe is arranged in the screw hole in a threaded mode, a water bath box is fixedly arranged on the surface of the external thread pipe, a clamping assembly is fixedly arranged on the top of the water bath box, and an exosome reagent pipe is arranged in the water bath box through the clamping assembly. When the temperature reaches the use, solenoid valve one is closed, carry the rivers to the connecting pipe inside through conveying component to in entering the water bath through business turn over water pipe, connecting pipe and external screw thread pipe, be convenient for circulate the use to the rivers that the temperature is less than the exosome user demand, avoided the water resource to appear extravagant, avoided the temperature to be less than the heat extravagant in the warm water that the exosome used, thereby make exosome detection device more environmental protection, and make exosome detection device use more convenient through automatic heating and carrying to rivers.
Description
Technical Field
The invention relates to the technical field of exosome detection, in particular to an exosome detection device.
Background
Exosomes refer to small vesicles comprising complex RNAs and proteins, which nowadays are specifically disc vesicles with diameters of 40-100nm, and a variety of cells can secrete exosomes under normal and pathological conditions. The method mainly comprises the steps of preparing warm water, immersing a test tube containing the exosomes in the warm water for detection, and carrying out detection on the exosomes by using professional equipment to ensure the activity of the exosomes.
However, after the use, the temperature of the warm water can be reduced, so that the temperature of the warm water cannot reach the temperature range required by the exosome, therefore, the warm water can be directly used as wastewater to be removed, and the warm water still has the temperature although reaching the temperature required by the exosome, so that the waste of water resources can be caused by directly removing the warm water with the temperature reaching the temperature, and the exosome detection device is provided.
Disclosure of Invention
The invention provides an exosome detection device for solving the problems in the background art.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
The utility model provides an exosome detection device, includes the protective housing, the fixed mount pad that is provided with of protective housing lower side wall, the mount pad top runs through and is provided with the screw, the inside screw thread of screw is provided with the external screw thread pipe, external screw thread pipe fixed surface is provided with the water bath case, the fixed clamping assembly that is provided with in water bath case top, the inside exosome reagent pipe that is provided with through the clamping assembly of water bath case, water bath case top is fixedly provided with level sensor one, level sensor one's detection end runs through the water bath case and extends to the inside of water bath case, the fixed heating cabinet that is provided with in protective housing bottom, the inside fixed heated board one that is provided with of heating cabinet, the inside heating component that is provided with of heated board one is provided with of heated board, the heating box surface is provided with the closing plate through fixed subassembly, closing plate right side fixedly provided with the heated board two, two detection ends of water bath case run through case and extend to inside the inside pipe, level sensor one is provided with the inside pipe, the inside pipe is connected to the inside pipe that is provided with of fixed pipeline, the inside pipe is connected to the inside pipe that is provided with of conveying pipeline, the inside pipe is connected to the inside pipe that is provided with the inside pipe that passes through the fixed pipeline, the inside pipe is passed through the fixed mounting of the inside pipe.
Preferably, the clamping assembly comprises a mounting plate arranged at the top of the water bath tank, a movable rod is arranged in the mounting plate in a sliding manner, a limiting plate is fixedly arranged at one end of the movable rod, a clamping plate is fixedly arranged at the other end of the movable rod, a spring is fixedly arranged on one side, close to the movable rod, of the clamping plate, the spring is fixedly connected with the mounting plate, a protection pad is fixedly arranged in the clamping plate, and the protection pad and the exosome reagent tube are in lap joint.
Preferably, the heating assembly comprises a heat insulation plate arranged in the heat insulation plate, and a heating rod is fixedly arranged between the opposite surfaces of the heat insulation plate.
Preferably, the fixed subassembly is including setting up in the fixed plate on heating cabinet surface, fixed plate fixed mounting is on the closing plate surface, the fixed slot has been seted up on the fixed plate surface, the inside overlap joint of fixed slot is provided with the fixed column, fixed column fixed mounting is on the surface of heating cabinet, fixed column surface screw thread is provided with the screw cap.
Preferably, the conveying assembly comprises a fixing seat arranged on the right side of the heating box, a conveying pump is fixedly arranged at the top of the fixing seat, a water inlet pipe is fixedly arranged at the water inlet end of the conveying pump, the left end of the water inlet pipe penetrates through the heating box and extends to the inside of the heating box, a water outlet pipe is fixedly arranged at the water outlet end of the conveying pump, a solenoid valve II is fixedly arranged on the water outlet pipe, and the water outlet pipe is fixedly connected with the connecting pipe.
Preferably, the protection casing is rectangular structure, the protection casing adopts transparent acrylic board to make, the protection casing does not all contact with mount pad and water bath.
Preferably, the number of the clamping plates and the number of the protection pads are two, the two clamping plates and the two protection pads are symmetrically distributed, and the protection pads are made of anti-skid rubber.
Preferably, the water bath box is circular structure, water bath box and mount pad are overlap joint setting, the water bath box adopts heat preservation material to make.
Preferably, a water adding pipe is arranged at the rear side of the heating box, and an electromagnetic one-way valve is fixedly arranged on the water adding pipe.
Compared with the prior art, the invention has the beneficial effects that:
This exosome detection device, add inside the heating cabinet through the water pipe that adds of heating cabinet rear side, make the heating cabinet store half case water, add the water bath incasement portion with the warm water, insert the exosome reagent pipe inside the water bath incasement portion, and it is fixed through the centre gripping subassembly, and detect the degree of depth that exosome reagent pipe was male to detect the water level through liquid level sensor one, after the warm water uses completely, take out exosome reagent pipe from the water bath incasement, open solenoid valve one, make the rivers in the water bath incasement get into the heating cabinet inside through external screw thread pipe, connecting pipe and water inlet and outlet pipe, and heat through heating subassembly, detect the condition of rivers through liquid level sensor two and temperature sensor simultaneously, when the temperature reaches the use, solenoid valve one is closed, carry the rivers to inside the connecting pipe through the delivery assembly, and get into the incasement through business turn over water pipe, the rivers of the exosome use demand of being less than the exosome use of temperature is convenient for circulate and use, waste has avoided the water resource appearance, the heat in the warm water that the temperature is less than the exosome use is wasted, thereby make the rivers examine the environment-friendly and make the water more convenient to use of the device to use the external health care.
This exosome detection device, through setting up of mounting panel, carriage release lever, limiting plate, grip block, spring and protection pad, be convenient for carry out the centre gripping fixedly to exosome reagent pipe to the position of putting into the water bath incasement to exosome reagent pipe is spacing, has avoided the exosome reagent pipe to appear floating in the testing process and has led to the effect decline of warm water bath.
This exosome detection device, through the setting of delivery pump, outlet pipe, solenoid valve two and fixing base, be convenient for carry the rivers after accomplishing the heating to be convenient for take out the rivers after accomplishing the heating from the heating cabinet to inside the connecting pipe, thereby be convenient for warm water passes through connecting pipe, business turn over water pipe, connecting pipe and external screw thread pipe entering water bath incasement portion.
Drawings
FIG. 1 is an isometric view of a structure of the present invention;
FIG. 2 is a front cross-sectional view of the structure of the present invention;
FIG. 3 is a schematic view of a mounting base of the present invention;
FIG. 4 is an enlarged view of the structure at A of FIG. 2;
FIG. 5 is a schematic view of a partial structure of the present invention
FIG. 6 is a right cross-sectional view of the structure of the present invention
FIG. 7 is a schematic view of another partial structure of the present invention
FIG. 8 is a schematic structural view of the structural fastening member of the present invention.
In the figure: 1a protection shell, 2 a mounting seat, 3 screw holes, 4 external thread pipes, 5a water bath box, 6a mounting plate, 7a moving rod, 8a limiting plate, 9 a clamping plate, 10 springs, 11 a protection pad, 12 an exosome reagent pipe, 13 a liquid level sensor I, 14 a heating box, 15 a heat preservation plate I, 16 a fixing plate, 17 a fixing groove, 18 a fixing column, 19 thread caps, 20 a sealing plate, 21 a heat preservation plate II, 22 a temperature sensor, 23 a heat preservation plate, 24 a heating rod, 25 a conveying pump, 26 a water outlet pipe, 27 a solenoid valve II, 28 a connecting pipe, 29 a water inlet and outlet pipe, 30 a solenoid valve I, 31 a connecting pipe, 32 a fixing seat, 33 a liquid level sensor II and 34 a water inlet pipe.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to FIGS. 1-8, an exocrine body detecting device comprises a protective shell 1, the protective shell 1 is of a rectangular structure, the protective shell 1 is made of transparent acrylic plates, the protective shell 1 is not contacted with an installation seat 2 and a water bath box 5, the condition inside the protective shell is convenient to observe through the protective shell 1, the installation seat 2 is fixedly arranged on the lower side wall of the protective shell 1, a screw hole 3 is formed in the top of the installation seat 2 in a penetrating way, an external thread pipe 4 is arranged on the internal thread of the screw hole 3, a water bath box 5 is fixedly arranged on the surface of the external thread pipe 4, the water bath box 5 is of a circular structure, the water bath box 5 and the installation seat 2 are in lap joint, the water bath box 5 is made of heat preservation materials, warm water entering the water bath box 5 is convenient to preserve heat, the temperature of the warm water is prevented from falling too fast, the top of the water bath box 5 is fixedly provided with a clamping component, the inside of the water bath 5 is provided with an exosome reagent pipe 12 through a clamping component, the top of the water bath 5 is fixedly provided with a first liquid level sensor 13, the detection end of the first liquid level sensor 13 penetrates through the water bath 5 and extends into the water bath 5, the bottom of the protective shell 1 is fixedly provided with a heating box 14, the inside of the heating box 14 is fixedly provided with a first heat insulation board 15, the inside of the first heat insulation board 15 is provided with a heating component, the surface of the heating box 14 is provided with a fixed component, the surface of the heating box 14 is provided with a sealing board 20 through the fixed component, the right side of the sealing board 20 is fixedly provided with a second heat insulation board 21, the right side of the sealing board 20 is fixedly provided with a temperature sensor 22, the top of the heating box 14 is fixedly provided with a second liquid level sensor 33, the detection end of the second liquid level sensor 33 penetrates through the heating box 14 and extends into the inside of the heating box 14, the rear side of the heating box 14 is provided with a water adding pipe, and the water adding pipe is fixedly provided with an electromagnetic one-way valve, the water flow is convenient to be added into the heating box 14 through the water adding pipe, the reverse suction phenomenon of the water adding pipe is convenient to be prevented through the electromagnetic one-way valve, the conveying component is arranged on the right side of the heating box 14, the connecting pipe 28 is arranged at the top of the heating box 14 through the conveying component, the water inlet pipe 29 and the water outlet pipe 29 are fixedly arranged in the connecting pipe 28, the electromagnetic valve I30 is fixedly arranged on the water inlet pipe 29, the connecting pipe 31 is fixedly arranged in the external thread pipe 4 in a lap joint manner, warm water is added into the heating box 14 through the water adding pipe at the rear side of the heating box 14, half water is stored in the heating box 14, the warm water is added into the water bath box 5, the exosome reagent pipe 12 is inserted into the water bath box 5 and is fixed through the clamping component, the depth of the insertion of the exosome reagent pipe 12 is detected through the first 13 detection water level of the liquid level sensor, when the warm water is used, the external secretion reagent pipe 12 is taken out from the water bath tank 5, the electromagnetic valve I30 is opened, so that water flow in the water bath tank 5 enters the heating tank 14 through the external thread pipe 4, the connecting pipe 31 and the water inlet and outlet pipe 29 and is heated by the heating component, meanwhile, the condition of the water flow is detected through the liquid level sensor II 33 and the temperature sensor 22, when the water temperature reaches the use condition, the electromagnetic valve I30 is closed, the water flow is conveyed into the connecting pipe 28 through the conveying component and enters the water bath tank 5 through the water inlet and outlet pipe 29, the connecting pipe 31 and the external thread pipe 4, the water flow with the temperature lower than the use requirement of the external secretion is conveniently recycled, the waste of water resources is avoided, the waste of heat in the warm water with the temperature lower than the use condition of the external secretion is avoided, and the external secretion detection device is more environment-friendly, and the exosome detection device is more convenient to use by automatically heating and conveying water flow.
Specifically, the clamping assembly is including setting up in the mounting panel 6 at water bath 5 top, the inside slip of mounting panel 6 is provided with movable rod 7, movable rod 7 one end is fixed and is provided with limiting plate 8, the other end of movable rod 7 is fixed and is provided with clamping plate 9, clamping plate 9 is close to the fixed spring 10 that is provided with in one side of movable rod 7, spring 10 and mounting panel 6 fixed connection set up, clamping plate 9 inside is fixed and is provided with protection pad 11, the quantity of clamping plate 9 and protection pad 11 is two, and two clamping plates 9 and two protection pads 11 all are symmetrical distribution, protection pad 11 adopts anti-skidding rubber to make, be convenient for protect exosome reagent pipe 12 through protection pad 11, the protection pad 11 that makes exosome reagent pipe 12 centre gripping through anti-skidding rubber is more firm, protection pad 11 and exosome reagent pipe 12 are lap setting, through mounting panel 6, movable rod 7, limiting plate 8, clamping plate 9, spring 10 and protection pad 11's setting, the exosome reagent pipe 12 of being convenient for carries out the centre gripping fixedly, thereby the water bath effect has been avoided the exosome reagent pipe 12 to put into the position of detecting in the water bath 5, the water bath effect has been avoided to appear in the water bath.
Specifically, the heating element includes the heat insulating board 23 that sets up in the inside of heated board one 15, and the fixed heating rod 24 that is provided with between the heat insulating board 23 opposite surfaces is convenient for heat the rivers in the heating cabinet 14 through the setting of heating rod 24.
Specifically, fixed subassembly is including setting up in the fixed plate 16 on heating cabinet 14 surface, fixed plate 16 fixed mounting is at closing plate 20 surface, fixed slot 17 has been seted up on fixed plate 16 surface, the inside overlap joint of fixed slot 17 is provided with fixed column 18, fixed column 18 fixed mounting is at the surface of heating cabinet 14, fixed column 18 surface screw thread is provided with screw cap 19, take off screw cap 19, the pulling fixed plate 16 drives closing plate 20 and removes for closing plate 20 drives heated board two 21 and shifts out in heating cabinet 14, thereby be convenient for maintain heating subassembly, the condition that the damage can't heat rivers has been avoided heating subassembly to appear.
Specifically, the conveying assembly includes fixing base 32 on the right side of be provided with heating cabinet 14, fixing base 32 top is fixed to be provided with delivery pump 25, delivery pump 25 water inlet end is fixed to be provided with inlet tube 34, the left end of inlet tube 34 runs through heating cabinet 14 and extends to inside the heating cabinet 14, delivery pump 25 water outlet end is fixed to be provided with outlet pipe 26, fixedly on the outlet pipe 26 be provided with solenoid valve two 27, outlet pipe 26 and connecting pipe 28 fixed connection set up, through the setting of delivery pump 25, outlet pipe 26, solenoid valve two 27 and fixing base 32, be convenient for carry the rivers after the heating is accomplished, thereby be convenient for take out the rivers after the heating is accomplished from heating cabinet 14 to inside connecting pipe 28, thereby be convenient for warm water through connecting pipe 28, business turn over water pipe 29, connecting pipe 31 and external screw thread pipe 4 get into inside the water bath cabinet 5.
When in use: the warm water is added into the heating box 14 through the water adding pipe at the rear side of the heating box 14, half water is stored in the heating box 14, the warm water is added into the water bath box 5, the exosome reagent pipe 12 is inserted into the water bath box 5, the exosome reagent pipe 12 pushes the clamping plate 9 to move through the protection pad 11, the movable rod 7 is pushed by the clamping plate 9 to drive the limiting plate 8 to move, the clamping plate 9 compresses the spring 10, the water level pinched by the water bath box 5 is detected through the first liquid level sensor 13, when the water level in the water bath box 5 reaches a preset value, the exosome reagent pipe 12 is stopped pushing, the clamping plate 9 pushes the protection pad 11 to be in close contact with the exosome reagent pipe 12 through the rebound action of the spring 10, after the warm water is used, the exosome reagent pipe 12 is taken out from the water bath box 5, the first electromagnetic valve 30 is opened, so that the water flow in the water bath tank 5 enters the connecting conduit 31 through the external thread pipe 4 and enters the water inlet and outlet pipe 29 through the connecting conduit 31, enters the heating tank 14 through the water inlet and outlet pipe 29, detects the water level in the heating tank 14 through the liquid level sensor II 33, heats the water flow through the heating rod 24 arranged in the heating assembly and detects the water temperature through the temperature sensor 22 when the liquid level sensor II 33 detects that the water level in the heating tank 14 is no longer changed, closes the electromagnetic valve I30 when the temperature sensor 22 detects that the water temperature reaches the use temperature, stops heating the water flow, simultaneously opens the electromagnetic valve II 27 arranged in the conveying assembly, starts the conveying pump 25, pumps the warm water in the heating tank 14 into the water outlet pipe 26 through the water inlet pipe 34, enters the connecting pipe 28 through the water outlet pipe 26, enters the water inlet and outlet pipe 29 through the connecting pipe 28, through the water inlet and outlet pipe 29 into the connecting conduit 31 and finally into the water bath 5 through the external thread pipe 4.
To sum up, this exosome detection device, add heating cabinet 14 inside with warm water through the water pipe that adds of heating cabinet 14 rear side, make the heating cabinet 14 store half case water, add inside the water bath 5 with warm water, insert the exosome reagent pipe 12 inside the water bath 5, and it is fixed through the centre gripping subassembly, and detect the degree of depth that exosome reagent pipe 12 was male through level sensor one 13, after warm water use is accomplished, take out exosome reagent pipe 12 from the water bath 5, open solenoid valve one 30, make the rivers in the water bath 5 get into inside the heating cabinet 14 through external screw thread pipe 4, connecting pipe 31 and business turn over water pipe 29, and detect through heating assembly, simultaneously detect the condition of rivers through level sensor two 33 and temperature sensor 22, solenoid valve one 30 is closed, carry the water flow to inside connecting pipe 28 through conveying assembly, and detect the exosome reagent pipe 12 male screw thread pipe 4 into in the water bath 5, be convenient for use the temperature is less than exosome use demand's is gone up from water bath 5, thereby the exosome water flow is avoided the temperature to the water pipe 29, the environmental protection is used in the environmental protection device has been avoided and the environmental protection to use the water waste is more to the environmental protection device, thereby use the environmental protection is more convenient to use in the water use the environmental protection device has been avoided in the water waste to use the temperature to use the water, and the environmental protection device is more used for the problem is avoided in the use.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides an exocrine body detection device, includes protection casing (1), its characterized in that, protection casing (1) lower lateral wall is fixed to be provided with mount pad (2), mount pad (2) top runs through and is provided with screw (3), screw (3) inside screw thread is provided with external screw thread pipe (4), external screw thread pipe (4) fixed surface is provided with water bath (5), water bath (5) top is fixed and is provided with clamping assembly, water bath (5) inside is provided with exocrine body reagent pipe (12) through clamping assembly, water bath (5) top is fixed and is provided with liquid level sensor one (13), liquid level sensor one (13)'s detection end runs through water bath (5) and extends to the inside of water bath (5), protection casing (1) bottom is fixed and is provided with heating cabinet (14), heating cabinet (14) inside is fixed and is provided with heat preservation board one (15), inside is provided with heating assembly, heating cabinet (14) surface is provided with fixed subassembly, water bath (5) inside is provided with liquid level sensor one (20) through clamping assembly, sealing plate (20) are fixed to be provided with sealing plate (20) right side seal plate (20), the utility model discloses a liquid level sensor, which comprises a heating box (14), and is characterized in that a liquid level sensor II (33) is fixedly arranged at the top of the heating box (14), the detection end of the liquid level sensor II (33) penetrates through the heating box (14) and extends into the heating box (14), a conveying assembly is arranged on the right side of the heating box (14), a connecting pipe (28) is arranged at the top of the heating box (14) through the conveying assembly, a water inlet pipe (29) is fixedly arranged in the connecting pipe (28), a solenoid valve I (30) is fixedly arranged on the water inlet pipe (29), a connecting pipe (31) is arranged in the water inlet pipe (29) in a lap joint mode, and the connecting pipe (31) is fixedly arranged in an external thread pipe (4);
The clamping assembly comprises a mounting plate (6) arranged at the top of the water bath box (5), a movable rod (7) is arranged in the mounting plate (6) in a sliding manner, a limiting plate (8) is fixedly arranged at one end of the movable rod (7), a clamping plate (9) is fixedly arranged at the other end of the movable rod (7), a spring (10) is fixedly arranged at one side, close to the movable rod (7), of the clamping plate (9), the spring (10) is fixedly connected with the mounting plate (6), a protection pad (11) is fixedly arranged in the clamping plate (9), and the protection pad (11) and an exosome reagent tube (12) are in lap joint;
the conveying assembly comprises a fixing seat (32) arranged on the right side of the heating box (14), a conveying pump (25) is fixedly arranged at the top of the fixing seat (32), a water inlet pipe (34) is fixedly arranged at the water inlet end of the conveying pump (25), the left end of the water inlet pipe (34) penetrates through the heating box (14) and extends into the heating box (14), a water outlet pipe (26) is fixedly arranged at the water outlet end of the conveying pump (25), a solenoid valve two (27) is fixedly arranged on the water outlet pipe (26), and the water outlet pipe (26) is fixedly connected with a connecting pipe (28).
2. An exosome detection apparatus according to claim 1, wherein the heating assembly comprises a heat insulation board (23) disposed inside the first heat insulation board (15), and a heating rod (24) is fixedly disposed between opposite surfaces of the heat insulation board (23).
3. The exosome detection device according to claim 1, characterized in that, the fixed subassembly is including setting up fixed plate (16) on heating cabinet (14) surface, fixed plate (16) fixed mounting is on closing plate (20) surface, fixed slot (17) have been seted up on fixed plate (16) surface, the inside overlap joint of fixed slot (17) is provided with fixed column (18), fixed column (18) fixed mounting is on heating cabinet (14) surface, fixed column (18) surface screw thread is provided with screw cap (19).
4. The exosome detection device according to claim 1, wherein the protection casing (1) is in a rectangular structure, the protection casing (1) is made of a transparent acrylic plate, and the protection casing (1) is not contacted with the mounting seat (2) and the water bath tank (5).
5. The exosome detection device according to claim 1, wherein the number of the clamping plates (9) and the protection pads (11) is two, and the two clamping plates (9) and the two protection pads (11) are symmetrically distributed, and the protection pads (11) are made of anti-slip rubber.
6. The exosome detection device according to claim 1, wherein the water bath box (5) is of a circular structure, the water bath box (5) and the mounting seat (2) are arranged in a lap joint manner, and the water bath box (5) is made of a heat insulation material.
7. An exosome detection apparatus according to claim 1, wherein a water adding pipe is provided at the rear side of the heating box (14), and an electromagnetic one-way valve is fixedly provided on the water adding pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410240136.3A CN117825673B (en) | 2024-03-04 | 2024-03-04 | Exosome detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410240136.3A CN117825673B (en) | 2024-03-04 | 2024-03-04 | Exosome detection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117825673A CN117825673A (en) | 2024-04-05 |
| CN117825673B true CN117825673B (en) | 2024-05-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410240136.3A Active CN117825673B (en) | 2024-03-04 | 2024-03-04 | Exosome detection device |
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| CN (1) | CN117825673B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2322618A1 (en) * | 1998-03-11 | 1999-09-16 | Cell Activation, Inc. | Methods of diagnosis and triage using cell activation measures |
| WO2009130649A2 (en) * | 2008-04-21 | 2009-10-29 | Nanoxis Ab | Plasma membrane vesicles and methods of making and using same |
| CN102846474A (en) * | 2011-07-01 | 2013-01-02 | 沈阳药科大学 | Device and method for preparing vesica with inner and outer water phase gradient difference |
| DE102013106380A1 (en) * | 2012-06-20 | 2013-12-24 | Sony Corporation | Liquid-based sensor device, useful for detecting the presence of analyte e.g. bacteria cell, comprises substrates, liquid crystal layer, and phospholipid layer, reaction layer, electrodes, and insulating layer and/or alignment layer |
| KR20140129491A (en) * | 2013-04-30 | 2014-11-07 | 김대홍 | Water saving temperature control system |
| WO2018137141A1 (en) * | 2017-01-24 | 2018-08-02 | 深圳华大基因研究院 | Exosomal dna-based method for performing non-invasive prenatal diagnosis and application thereof |
| CN109116003A (en) * | 2018-09-26 | 2019-01-01 | 扬州大学 | A kind of asphalt uniaxial penetration test device of water bath with thermostatic control |
| CN113549461A (en) * | 2021-07-19 | 2021-10-26 | 东南大学 | A kind of super-resolution imaging probe of exosome surface membrane protein and its preparation method and application |
| CN116064384A (en) * | 2021-11-04 | 2023-05-05 | 北京华龛生物科技有限公司 | Exosome separation method and separation system |
-
2024
- 2024-03-04 CN CN202410240136.3A patent/CN117825673B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2322618A1 (en) * | 1998-03-11 | 1999-09-16 | Cell Activation, Inc. | Methods of diagnosis and triage using cell activation measures |
| WO2009130649A2 (en) * | 2008-04-21 | 2009-10-29 | Nanoxis Ab | Plasma membrane vesicles and methods of making and using same |
| CN102846474A (en) * | 2011-07-01 | 2013-01-02 | 沈阳药科大学 | Device and method for preparing vesica with inner and outer water phase gradient difference |
| DE102013106380A1 (en) * | 2012-06-20 | 2013-12-24 | Sony Corporation | Liquid-based sensor device, useful for detecting the presence of analyte e.g. bacteria cell, comprises substrates, liquid crystal layer, and phospholipid layer, reaction layer, electrodes, and insulating layer and/or alignment layer |
| KR20140129491A (en) * | 2013-04-30 | 2014-11-07 | 김대홍 | Water saving temperature control system |
| WO2018137141A1 (en) * | 2017-01-24 | 2018-08-02 | 深圳华大基因研究院 | Exosomal dna-based method for performing non-invasive prenatal diagnosis and application thereof |
| CN109116003A (en) * | 2018-09-26 | 2019-01-01 | 扬州大学 | A kind of asphalt uniaxial penetration test device of water bath with thermostatic control |
| CN113549461A (en) * | 2021-07-19 | 2021-10-26 | 东南大学 | A kind of super-resolution imaging probe of exosome surface membrane protein and its preparation method and application |
| CN116064384A (en) * | 2021-11-04 | 2023-05-05 | 北京华龛生物科技有限公司 | Exosome separation method and separation system |
Non-Patent Citations (1)
| Title |
|---|
| 外泌体β-actin mRNA在恶性胸腔积液、结核性胸腔积液鉴别诊断中的应用价值;农雪萍等;《中国医学创新》;20191231;第16卷(第29期);第168-172页 * |
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