CN118425031A - Multi-wavelength transmission and scattering integrated optical detection device - Google Patents

Abstract

The application provides a multi-wavelength transmission and scattering integrated optical detection device. The multi-wavelength transmission scattering integrated optical detection device comprises: a light source unit including a first light source, a second light source, and a first dichroic mirror, wherein light emitted from the first light source can pass through the first dichroic mirror, light emitted from the second light source is reflected by the first dichroic mirror and is converged into light emitted from the first light source, the first light source and the second light source are both cold light sources, and wavelengths of the emitted light are different; and the detection part comprises a reaction cup mounting position, a fixing seat, a transmission detection sensor and a scattering detection sensor, wherein the reaction cup mounting position is arranged in the fixing seat, the transmission detection sensor is arranged on the end face of the fixing seat, which is perpendicular to the light emitted by the first light source and far away from the light source part, and the scattering detection sensor is arranged on the end face of the fixing seat, which is parallel to the light emitted by the first light source.

Description

Multi-wavelength transmission and scattering integrated optical detection device

Technical Field

The present application relates to the field of medical equipment, and in particular to a multi-wavelength transmission and scattering integrated optical detection device for use in detection equipment such as specific protein analyzers.

Background technique

The specific protein analyzer is an analytical instrument used to measure the content of specific proteins in human body fluids. It uses the principle of laser scattering turbidimetry to measure the turbidity of the solution after the reaction of the sample and reagent in the reaction cup to determine the level of specific protein content in the sample. The level of specific protein content reflects the corresponding state of the patient's body and provides effective reference data for doctors' diagnosis.

The patent with the announcement number CN212658616U discloses an assembly structure of an optical path detection component of a urine specific protein analyzer, whose light source is a halogen lamp. The halogen lamp is a broad spectrum light source. In order to provide a specific wavelength of detection light for the reaction cup, it is also necessary to configure relatively complex optical devices, such as the spherical mirror, filter assembly, or grating monochromator for generating monochromatic light mentioned in the patent. The overall structure is relatively complex and large in size, and is not suitable for portable small devices.

In addition, halogen lamps are heat light sources, and their lifespan is generally less than 2,000 hours. They need to be replaced regularly, and the maintenance cost is relatively high. Halogen lamps need 20-30 minutes of stabilization time after lighting, and then they can output normally and stably. Therefore, during testing, especially when multiple reaction cups are tested continuously, the halogen lamps need to be continuously lit, making the halogen lamps less durable. Halogen lamps with heat light sources may also affect the ambient temperature, such as increasing the temperature of the reaction cup, thereby affecting the accuracy of the test.

Summary of the invention

In order to solve or improve at least one of the problems mentioned in the background technology, the present application provides a multi-wavelength transmission and scattering integrated optical detection device.

The multi-wavelength transmission and scattering integrated optical detection device provided by the present application comprises:

a light source unit, the light source unit comprising a first light source, a second light source, and a first dichroic mirror, the light emitted by the first light source can pass through the first dichroic mirror, the light emitted by the second light source is reflected by the first dichroic mirror and merges into the light emitted by the first light source, the first light source and the second light source are both cold light sources and emit light of different wavelengths; and

The detection part includes a reaction cup mounting position, a fixing seat, a transmission detection sensor and a scattering detection sensor. The reaction cup mounting position is arranged in the fixing seat, the transmission detection sensor is arranged on an end face of the fixing seat which is perpendicular to the light emitted by the first light source and away from the light source part, and the scattering detection sensor is arranged on an end face of the fixing seat which is parallel to the light emitted by the first light source.

In at least one embodiment, the first light source is a semiconductor laser emitter or a light emitting diode, and the second light source is a semiconductor laser emitter or a light emitting diode.

In at least one embodiment, the light source portion includes a dichroic mirror mounting seat, the dichroic mirror mounting seat includes a mirror portion, the mirror portion includes an inclined surface inclined relative to the light emitted by the first light source, the first dichroic mirror abuts against the inclined surface, or the inclined surface has a groove, and the first dichroic mirror is arranged in the groove.

In at least one embodiment, a first light source filter is disposed between the first light source and the first dichroic mirror.

In at least one embodiment, a second light source filter is disposed between the second light source and the first dichroic mirror.

In at least one embodiment, the detection unit further includes an incubation module, the incubation module is disposed in the fixing seat, and a recessed portion is disposed in the incubation module, and the recessed portion is a mounting position for the reaction cup.

The incubation module includes a heating plate so that the temperature of the reaction cup installed in the reaction cup installation position can be controlled.

In at least one embodiment, the incubation module further comprises a frame structure and a heat insulating sleeve wrapped around the outside of the frame structure.

The heating plate is arranged between the frame structure and the heat insulation sleeve, the heat insulation sleeve is against the fixing seat, and the frame structure has the recessed part.

In at least one embodiment, a focusing mirror is disposed between the reaction cup mounting position and the scattering detection sensor.

In at least one embodiment, the scattering detection sensor is a side scattering detection sensor arranged on the side of the fixing base, or

The scattering detection sensor is a bottom surface scattering detection sensor arranged on the bottom surface of the fixing seat.

In at least one embodiment, the light source unit further includes a third light source and a second dichroic mirror, and the light emitted by the third light source can be merged into the light emitted by the first light source and the light emitted by the second light source after being reflected by the second dichroic mirror.

The present application abandons the traditional hot light source halogen lamp and uses a cold light source, avoiding the problems of short life, high maintenance cost and possible impact on ambient temperature caused by the hot light source. The present application realizes the superposition of monochromatic light of different wavelengths by setting a dichroic mirror, has a simple structure, is easy to replace, and is better adapted to small devices.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 shows a schematic structural diagram of a multi-wavelength transmission and scattering integrated optical detection device according to an embodiment of the present application.

FIG2 shows a schematic diagram of the internal structure of a multi-wavelength transmission and scattering integrated optical detection device according to an embodiment of the present application.

FIG3 shows a schematic diagram of the optical path structure of a multi-wavelength transmission and scattering integrated optical detection device according to an embodiment of the present application.

FIG. 4 is a schematic structural diagram of a multi-wavelength transmission and scattering integrated optical detection device from one viewing angle according to an embodiment of the present application.

FIG5 is a schematic structural diagram of a multi-wavelength transmission and scattering integrated optical detection device from another perspective according to an embodiment of the present application.

Description of Reference Numerals

1 light source portion; 11 first light source; 111 first light source filter; 12 second light source; 121 second light source filter; 13 third light source; 131 third light source filter; 14 first dichroic mirror; 15 second dichroic mirror; 16 light shield; 17 dichroic mirror mounting seat; 171 mirror portion; 1711 inclined surface; 1712 light hole; 172 connecting portion;

2 detection unit; 21 reaction cup installation position; 22 incubation module; 23 fixing seat; 24 transmission detection sensor; 25 scattering detection sensor; 251 side scattering detection sensor; 252 bottom scattering detection sensor; 26 focusing mirror;

3 reaction cups

Detailed ways

The exemplary embodiments of the present application are described below with reference to the accompanying drawings. It should be understood that these specific descriptions are only used to teach those skilled in the art how to implement the present application, and are not intended to exhaust all possible methods of the present application, nor to limit the scope of the present application.

The embodiments of the present application provide a multi-wavelength transmission and scattering integrated optical detection device, which is sometimes referred to as an “optical detection device” hereinafter. Multi-wavelength means that the detection light can superimpose monochromatic light of at least two wavelengths.

1 and 3 , the optical detection device may include a light source unit 1 and a detection unit 2. The light source unit 1 is used to provide detection light, a reaction cup may be placed in the detection unit 2, and the detection light is transmitted and scattered through the reaction cup 3 into a corresponding sensor (described later) connected to the detection unit 2.

The light source unit 1 may include at least two light sources with different wavelengths and a dichroic mirror. One light source may pass through the dichroic mirror and enter the detection unit 2, and the other light source may be reflected by the dichroic mirror and enter the detection unit 2. The two light sources are arranged at different positions, but after integration by the dichroic mirror, the light emitted by the two light sources can overlap, thereby obtaining specific multi-wavelength light.

1 , 2 and 3 , taking the example that the light source unit 1 can superimpose monochromatic light of three wavelengths, the light source unit 1 includes a first light source 11 , a second light source 12 , a third light source 13 , a first dichroic mirror 14 and a second dichroic mirror 15 .

The direction of the light emitted by the first light source 11 is the direction of the optical axis, and the light emitted by the second light source 12 and the third light source 13 is perpendicular to the direction of the optical axis. The first dichroic mirror 14 is arranged in the optical axis, and the light of the second light source 12 is reflected by the first dichroic mirror 14 and then merged into the optical axis. Similarly, the second dichroic mirror 15 is arranged in the optical axis, and the light of the third light source 13 is reflected by the second dichroic mirror 15 and then merged into the optical axis. The light of the first light source 11, the second light source 12 and the third light source 13 can be superimposed to obtain specific multi-wavelength light. The angle between the first dichroic mirror 14 and the optical axis can be 45°, and the angle between the second dichroic mirror 15 and the optical axis can be 45°.

Of course, the number of light sources and dichroic mirrors can also be adjusted, for example, more light sources and dichroic mirrors can be added to achieve the superposition of more monochromatic lights; or the number of superimposed light sources can be adjusted by turning off one or more light sources. The light sources can also be turned on and off by time or wavelength according to specific detection requirements to meet, for example, single wavelength detection, dual wavelength, triple wavelength or other specific detection requirements.

2 , the light source unit 1 may include a dichroic mirror mounting seat 17. The dichroic mirror mounting seat 17 includes an integrally formed mirror portion 171 and a connecting portion 172. The mirror portion 171 includes an inclined surface 1711 inclined to the optical axis, and the dichroic mirror abuts against the inclined surface 1711, or a groove is provided on the inclined surface 1711, and the dichroic mirror is arranged in the groove. The inclined surface 1711 makes it easy to control the angle of the dichroic mirror. The mirror portion 171 has a light through hole 1712, and light can pass through the light through hole 1712 to pass through the dichroic mirror mounting seat 17.

A threaded hole may be provided in the connection portion 172 to facilitate assembly of the dichroic mirror mounting seat 17 with other structures (eg, a frame structure of an optical detection device).

The light source can be a cold light source such as a semiconductor laser or a light emitting diode. The continuous lighting life of cold light sources such as semiconductor lasers and light emitting diodes can usually reach more than 10,000 hours. No warm-up time is required, and the device can be turned on and tested immediately. Compared with thermal light sources, cold light sources have a longer service life and lower maintenance costs.

Exemplarily, the first light source 11 is a semiconductor laser (LD) with a wavelength of 650nm, the second light source is a light emitting diode (LED) with a wavelength of 565nm, and the third light source is a light emitting diode (LED) with a wavelength of 340nm. The first dichroic mirror 14 can transmit light with a wavelength of 650nm, and can reflect light with a wavelength of 565nm. The second dichroic mirror 15 can transmit light with wavelengths of 650nm and 565nm, and can reflect light with a wavelength of 340nm. Of course, the type of the light source can be adjusted.

Further, referring to FIG3 , the light source unit 1 may further include a first light source filter 111 disposed between the first light source 11 and the first dichroic mirror 14, and the first light source filter 111 is used to filter light other than a specific wavelength (e.g., 650 nm) emitted by the first light source 11. Of course, the light source unit 1 may further include a second light source filter 121 and a third light source filter 131. The second light source filter 121 is disposed between the second light source 12 and the first dichroic mirror 14, and the second light source filter 121 is used to filter light other than a specific wavelength (e.g., 565 nm) emitted by the second light source 12. The third light source filter 131 is disposed between the third light source 13 and the second dichroic mirror 15, and the third light source filter 131 is used to filter light other than a specific wavelength (e.g., 340 nm) emitted by the third light source 13.

The wavelength configuration of the detection light in this application is flexible, and specific light sources and dichroic mirrors can be assembled modularly, which is very suitable for small devices.

The light source unit 1 may further include a light shield 16 , which is wrapped around the outer side of the dichroic mirror mounting seat 17 to prevent external light from entering the detection unit and affecting the detection process.

2 and 4 , the detection unit 2 may include a cuvette mounting position 21 , an incubation module 22 , a fixing seat 23 , a transmission detection sensor 24 and a scattering detection sensor 25 .

The incubation module 22 is provided with a recessed portion, which is the cuvette mounting position 21. The incubation module 22 can heat the cuvette in the cuvette mounting position 21, so that the reactants in the cuvette are at a suitable incubation temperature, thereby making the reaction in the cuvette more stable and the detection result more accurate.

The incubation module 22 may include a frame structure, an insulation sleeve wrapped around the frame structure, and a heating plate disposed between the frame structure and the insulation sleeve. Exemplarily, the frame structure may be an aluminum frame, which is convenient for heat conduction so that the temperature of each position of the reaction cup mounting position 21 is relatively uniform. The incubation module 22 is installed in the fixing seat 23, and the insulation sleeve may be against the fixing seat 23 to prevent the external temperature from interfering with the reaction cup. The heating plate may be, for example, a PTC (positive temperature coefficient) heating plate. The insulation sleeve may be a PA66GF30 (PA66 plastic material plus 30% glass fiber) nylon sleeve.

A temperature sensor may also be provided in the incubation module 22 to detect the temperature of the reaction cup, so that the staff can timely correct the heating temperature of the incubation module 22. The temperature sensor may include a platinum resistor with a three-wire connection method.

4 and 5 , a transmission detection sensor 24 is provided on the end face of the fixing seat 23 perpendicular to the optical axis. Light along the optical axis can sequentially pass through the fixing seat 23 , the incubation module 22 , the reaction cup, the incubation module 22 , the fixing seat 23 and enter the transmission detection sensor 24 .

The end surface of the fixing seat 23 parallel to the optical axis can be provided with a scattering detection sensor 25, for example, the side surface (as shown in FIG. 4 ) or the bottom surface (as shown in FIG. 5 ) can be provided with a scattering detection sensor 25. Light along the optical axis direction can sequentially pass through the fixing seat 23 and the incubation module 22, and after being scattered in the reaction cup, pass through the incubation module 22 and the fixing seat 23 and enter the scattering detection sensor 25.

3 , a focusing mirror 26 can be installed between the reaction cup installation position 21 and the scattering detection sensor 25. The focusing mirror 26 can improve the ability of scattered light to enter the scattering detection sensor 25. For example, in the case of weak reaction or sensitive signal change, the focusing mirror 26 can be installed. The focusing mirror 26 can be an aspherical focusing mirror.

That is, the present application can simultaneously implement two detection modes, namely, transmission detection and scattering detection. The transmission signal and the scattering signal are completely separated in the signal receiving direction, so that the reception of the two optical paths does not interfere with each other.

In one embodiment of the present application, the scattering detection sensor 25 may be a side scattering detection sensor 251 disposed on a side of the fixing base 23. The side may be a left side or a right side.

In another embodiment of the present application, the scattering detection sensor 25 may be a bottom surface scattering detection sensor 252 disposed on the bottom surface of the fixing seat 23 .

The above is a preferred embodiment of the present application. It should be pointed out that for those skilled in the art, several improvements and modifications can be made without departing from the principles of the present application. These improvements and modifications should also be regarded as the scope of protection of the present application.

Claims (10)

1. A multi-wavelength transmission and scattering integrated optical detection device, comprising: A light source unit (1), the light source unit (1) comprising a first light source (11), a second light source (12), and a first dichroic mirror (14), the light emitted by the first light source (11) being able to pass through the first dichroic mirror (14), the light emitted by the second light source (12) being reflected by the first dichroic mirror (14) and merging into the light emitted by the first light source (11), the first light source (11) and the second light source (12) being both cold light sources and emitting light of different wavelengths; and A detection unit (2), the detection unit (2) comprising a reaction cup mounting position (21), a fixing seat (23), a transmission detection sensor (24) and a scattering detection sensor (25); the reaction cup mounting position (21) is arranged in the fixing seat (23); the transmission detection sensor (24) is arranged on an end surface of the fixing seat (23) which is perpendicular to the light emitted by the first light source (11) and away from the light source unit (1); and the scattering detection sensor (25) is arranged on an end surface of the fixing seat (23) which is parallel to the light emitted by the first light source (11). 2. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1, characterized in that the first light source (11) is a semiconductor laser emitter or a light emitting diode, and the second light source (12) is a semiconductor laser emitter or a light emitting diode. 3. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1 is characterized in that the light source part (1) includes a dichroic mirror mounting seat (17), the dichroic mirror mounting seat (17) includes a mirror portion (171), the mirror portion (171) includes an inclined surface (1711) inclined relative to the light emitted by the first light source (11), the first dichroic mirror (14) abuts against the inclined surface (1711), or the inclined surface (1711) has a groove, and the first dichroic mirror (14) is arranged in the groove. 4. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1, characterized in that a first light source filter (111) is arranged between the first light source (11) and the first dichroic mirror (14). 5. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1, characterized in that a second light source filter (121) is arranged between the second light source (12) and the first dichroic mirror (14). 6. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1, characterized in that the detection unit (2) further comprises an incubation module (22), the incubation module (22) is arranged in the fixing seat (23), and a recessed portion is provided in the incubation module (22), and the recessed portion is the reaction cup installation position (21), The incubation module (22) includes a heating plate so that the temperature of the reaction cup installed in the reaction cup installation position (21) can be controlled. 7. The multi-wavelength transmission and scattering integrated optical detection device according to claim 6, characterized in that the incubation module (22) further comprises a frame structure and a heat insulation sleeve wrapped around the outside of the frame structure, The heating plate is arranged between the frame structure and the heat insulation sleeve, the heat insulation sleeve abuts against the fixing seat (23), and the frame structure has the recessed portion. 8. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1, characterized in that a focusing mirror (26) is provided between the reaction cup mounting position (21) and the scattering detection sensor (25). 9. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1, characterized in that the scattering detection sensor (25) is a side scattering detection sensor (251) arranged on the side of the fixing seat (23), or The scattering detection sensor (25) is a bottom surface scattering detection sensor (252) arranged on the bottom surface of the fixing seat (23). 10. The multi-wavelength transmission and scattering integrated optical detection device according to claim 1 is characterized in that the light source part (1) also includes a third light source (13) and a second dichroic mirror (15), and the light emitted by the third light source (13) can be merged into the light emitted by the first light source (11) and the light emitted by the second light source (12) after being reflected by the second dichroic mirror (15).