CN119701476B - Extraction method and extraction equipment for plant exosomes - Google Patents
Extraction method and extraction equipment for plant exosomes Download PDFInfo
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- CN119701476B CN119701476B CN202510196559.4A CN202510196559A CN119701476B CN 119701476 B CN119701476 B CN 119701476B CN 202510196559 A CN202510196559 A CN 202510196559A CN 119701476 B CN119701476 B CN 119701476B
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Abstract
The invention discloses a method and equipment for extracting plant exosomes, which relate to the technical field of exosomes extraction and comprise two filter screens, wherein the two filter screens are symmetrically arranged up and down, the filter screens are arranged in a wave way, one side of the filter screen close to a fixed ring is provided with a filter hole, and the filter screen is located in the concave part of the filter screen, the top of the filter screen is provided with a through hole, the diameter of the upper hole of the filter screen is gradually increased from outside to inside, the design that the hole gradually becomes bigger can remove very tiny impurities in the initial stage of filtration, the through hole is arranged at the convex part of the filter screen, and the filter screen is oval. According to the plant exosome extraction method and the plant exosome extraction equipment, the wavy filter screen is arranged, so that a sample can have more contact area and more reasonable flow paths on the filter screen, and the filtering process is faster. Further, more liquid parts can be filtered in a shorter time, the filtering time is reduced, and the efficiency of the whole exosome extraction process is improved.
Description
Technical Field
The invention relates to the technical field of exosome extraction, in particular to a plant exosome extraction method and extraction equipment.
Background
Plant exosomes refer to extracellular vesicles of a specified size range secreted by plant cells in a narrow sense, but in recent years, researchers in various countries refer to a separation method of animal cell exosomes, a series of plant-derived nano vesicles or exosome-like nano particles prepared from plant samples are also included in the concept of exosomes, mainly derived from multi vesicles formed by invagination of intracellular lysosome particles, are released into extracellular matrixes after being fused with cell membranes through multi vesicle outer membranes, and various cells can secrete exosomes under normal and pathological conditions. The exosomes contain complex RNAs and proteins in vivo, all cultured cell types can secrete exosomes, and exosomes naturally occur in body fluids.
The existing plant exosome composition extraction device is capable of improving the extraction difficulty and reducing the extraction efficiency because plants are not completely crushed in the centrifugal extraction process of the plants, and meanwhile, the existing plant exosome composition extraction device is capable of cleaning the inside of the existing plant exosome composition after the use is finished, so that the cleaning quality cannot be guaranteed, and the working pressure of workers is greatly improved.
Disclosure of Invention
In order to achieve the above purpose, the invention is realized by the following technical scheme:
a method for extracting plant exosomes comprises the following steps:
s1, crushing plants, cleaning plant materials, and crushing plant tissues by using crushing equipment;
S2, screening and filtering, namely performing preliminary filtration on the crushed sample by using extraction equipment for removing larger impurities;
S3, centrifugal separation, namely, low-speed centrifugation, medium-speed centrifugation and high-speed centrifugation are carried out, and the supernatant fluid is enriched with exosomes;
s4, ultracentrifugation, namely transferring the supernatant after the ultracentrifugation into an ultracentrifugation tube, and precipitating exosomes at the bottom of the ultracentrifuge tube;
S5, washing and resuspension, namely washing the precipitate by using PBS buffer solution, and resuspension in a proper amount of PBS buffer solution to obtain exosome suspension.
The extraction equipment in the S2 comprises a frame body, wherein the top of the frame body is fixedly connected with a shell, the top of the shell is fixedly connected with a feed inlet, the top of the shell is fixedly connected with a motor, the output end of the motor penetrates through the shell, and the inside of the shell is fixedly connected with a cooling pipe;
the screening component is fixedly arranged in the shell;
The filter assembly is fixedly arranged in the shell and is positioned below the screening assembly;
The filter assembly comprises a rotating shaft, the rotating shaft is fixedly connected with the output end of the motor, the rotating shaft is rotationally connected with the shell, the outer side of the rotating shaft is rotationally connected with a fixed ring, and the outer side of the fixed ring is fixedly connected with an intermediate assembly;
preferably, the middle assembly comprises two filter screens, the two filter screens are arranged symmetrically up and down, a sample after preliminary filtration flows to the edge of the filter screen through the guide plate, then the sample flows towards the middle along the filter screen under the action of the gravity of the sample, and the wavy filter screens are arranged, so that the sample can have more contact areas and more reasonable flow paths on the filter screen, and the filtration process is faster. And then can filter more liquid parts in shorter time, reduce filtration time, the efficiency of whole exosome extraction flow has been improved, the filter screen is the wave setting, the filter screen has been seted up near one side of solid fixed ring, and the filter screen hole is located the concave part of filter screen, the through-hole has been seted up at the top of filter screen, the diameter in filter screen upper hole is from outside to interior increase gradually, the design that the hole grows gradually can remove very tiny impurity at the filtration initial stage, guarantee the purity degree of filtrate, then, in subsequent filtration process, can not hinder the passing through of exosome because the filter screen is too little again, the rate of recovery of exosome has been improved, both can intercept not unidimensional impurity, can let the exosome pass through smoothly again, have better filter effect to complicated plant sample, simultaneously the sample is in down flow the in-process, because the hole grows gradually, the velocity of flow also can suitably accelerate, the risk of jam has further been reduced, the through-hole sets up in the boss of filter screen, the filter screen hole is oval setting.
The inner chamber of casing is provided with the fixed block, the inclined plane that outside to middle part slope is established to the surface of fixed block, the tip fixedly connected with spacing ring of fixed block, the inside fixed connection of spacing ring and casing, the outside sliding connection of fixed block has the sliding ring, the cooling tube is located the interval department of sliding ring and casing, the top fixedly connected with guide plate of sliding ring, the guide plate is hopper form down, the fixed block is close to the surface of casing and has been seted up the round hole, the round hole runs through the fixed block, the spout has been seted up at the outside middle part of fixed block, the spout is trapezoidal setting, the inside sliding connection of spout has the stopper, the stopper runs through the casing, and stopper and casing sliding connection, the one end fixedly connected with locating component of casing is kept away from to the stopper, locating component runs through the fixed block, locating component and fixed block sliding connection.
Preferably, the one end fixedly connected with fixed frame of solid fixed ring is kept away from to the filter screen, the bottom fixedly connected with round bar of fixed frame, the round bar is located the inside of round hole, round bar passes through round hole and fixed block sliding connection, one side fixedly connected with spring that the fixed frame is close to the round bar, the sample after the preliminary filtration falls to the top of deflector through straining the jar, fall the top of filter screen along the deflector, under the gravity effect of sample self, the deflector atress drives the sliding ring and moves downwards, the top contact and the production extrusion of sliding ring and stopper this moment, make the stopper promote the round bar to keep away from the direction removal of stopper, the inclination of filter screen grow this moment, can accelerate filtration rate, avoid taking place to block up, the one end and the fixed block fixed connection of fixed frame are kept away from to the spring, the top fixedly connected with baffle that the fixed frame is close to the filter screen, the baffle is kept away from the baffle, the baffle and baffle are located same perpendicular.
Preferably, the shell comprises a shell, the outer side of the shell is fixedly connected with the frame body, the shell is close to the inner wall fixedly connected with the sloping plate of the screening assembly, the sloping plate is fixedly connected with the screening assembly, and a discharging hole is formed in the middle of the bottom of the shell.
Preferably, the locating component includes the extension rod, the one end fixed connection of shell is kept away from to extension rod and stopper, the sample after the preliminary filtration falls to the top of guide plate through straining a section of thick bamboo, fall to the top of filter screen along the guide plate, under the gravity effect of sample self, the guide plate atress drives the sliding ring and moves downwards, the sliding ring contacts and produces the extrusion with the top of stopper this moment, the stopper promotes the outside contact of the pivot at trapezoidal piece and middle part through the extension rod, frictional force between trapezoidal piece and the pivot grow for the rotational speed of pivot diminishes, and then make screening assembly filtration slowing down, make the sample that gets into the filter screen reduce, avoid sample pan feeding many, influence the purity of sample after the filtration, the one end fixedly connected with trapezoidal piece of stopper is kept away from to the extension rod, the logical groove has been seted up to the one end that the extension rod was kept away from to the trapezoidal piece.
Preferably, the screening subassembly includes the section of thick bamboo, the bottom fixed connection of section of thick bamboo and swash plate is strained to the inside of straining the section of thick bamboo, the inside of adding the shell through the feed inlet with the sample after smashing is strained to the inside that the sample got into along the swash plate, filter the section of thick bamboo through setting up, can carry out prefilter to the sample, and then can separate the exosome with great cell debris and impurity, motor external power supply work simultaneously, the motor drives the pivot and rotates, the pivot drives the revolving plate and the scraper blade rotates, make the inside sample of straining the section of thick bamboo rotate, the sample on the inner wall of straining the section of thick bamboo is cleaned to the revolving plate afterwards, prevent to strain the section of thick bamboo and take place to block up, can also improve the filter effect, the sample that the scraper blade drove the bottom upwards turns over, avoid the sample to pile up and influence and filter, revolving plate and pivot fixed connection are close to the inboard bottom fixedly connected with scraper blade of straining the section of thick bamboo, the scraper blade is the slope setting.
The invention provides a method and equipment for extracting plant exosomes. The beneficial effects are as follows:
1. According to the plant exosome extraction method and the plant exosome extraction equipment, the wavy filter screen is arranged, so that a sample can have more contact area and more reasonable flow paths on the filter screen, and the filtering process is faster. Further, more liquid parts can be filtered in a shorter time, the filtering time is reduced, and the efficiency of the whole exosome extraction process is improved.
2. According to the plant exosome extraction method and the plant exosome extraction equipment, through the design that the holes become larger gradually, impurities with different sizes can be intercepted, exosome can pass through smoothly, a good filtering effect is achieved on complex plant samples, meanwhile, the flow speed is accelerated properly due to the fact that the holes become larger gradually, and the risk of blockage is further reduced.
3. According to the plant exosome extraction method and the plant exosome extraction equipment, the trapezoidal blocks are pushed to be contacted with the outer side of the rotating shaft in the middle through the limiting blocks, friction force between the trapezoidal blocks and the rotating shaft is increased, the rotating speed of the rotating shaft is reduced, the screening assembly is further enabled to be filtered slowly, samples entering the filter screen are reduced, the phenomenon that the sample feeding amount is large, and the purity of the filtered samples is influenced is avoided.
4. According to the plant exosome extraction method and the plant exosome extraction equipment, the filter cartridge is arranged, the sample can be subjected to preliminary filtration, the exosome can be separated from larger cell fragments and impurities, meanwhile, the motor is externally connected with a power supply to work, the motor drives the rotating shaft to rotate, the rotating shaft drives the rotating plate and the scraping plate to rotate, so that the sample inside the filter cartridge is driven to rotate, and then the rotating plate cleans the sample on the inner wall of the filter cartridge, so that the filter cartridge is prevented from being blocked.
Drawings
FIG. 1 is a schematic flow chart of a method for extracting plant exosomes according to the present invention;
FIG. 2 is a schematic view showing the external structure of a plant exosome extraction apparatus according to the present invention;
FIG. 3 is a schematic cross-sectional view of the present invention;
FIG. 4 is a schematic top view of the present invention in semi-section;
FIG. 5 is a schematic view of a filter assembly according to the present invention;
FIG. 6 is a schematic view of a partial structure of a filter assembly according to the present invention;
FIG. 7 is a schematic cross-sectional view of an intermediate assembly of the present invention;
FIG. 8 is a schematic view of the housing structure of the present invention;
FIG. 9 is an enlarged schematic view of the positioning assembly of the present invention;
fig. 10 is a schematic view of the screen assembly of the present invention.
In the figure, 1, a frame body, 2, a shell, 21, a shell, 22, a sloping plate, 23, a discharging hole, 3, a feeding hole, 4, a motor, 5, a screening component, 51, a filter cartridge, 52, a rotating plate, 53, a scraping plate, 6, a filtering component, 61, a rotating shaft, 62, a fixed ring, 63, a middle component, 631, a filter screen, 632, a filtering hole, 633, a round rod, 634, a spring, 635, a through hole, 636, a baffle ring, 637, a baffle, 638, a fixed frame, 64, a deflector, 65, a fixed block, 66, a limiting block, 67, a positioning component, 671, an extension rod, 672, a trapezoid block, 673, a through groove, 68, a sliding groove, 69, a round hole, 610, a sliding ring, 611, a limiting ring, 7 and a cooling pipe are shown.
Detailed Description
The invention will be described in further detail with reference to the drawings and the detailed description. The embodiments of the invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
In a first embodiment, as shown in fig. 1 to 7, the present invention provides a technical solution:
a method for extracting plant exosomes comprises the following steps:
s1, crushing plants, cleaning plant materials, and crushing plant tissues by using crushing equipment;
S2, screening and filtering, namely performing preliminary filtration on the crushed sample by using extraction equipment for removing larger impurities;
S3, centrifugal separation, namely, low-speed centrifugation, medium-speed centrifugation and high-speed centrifugation are carried out, and the supernatant fluid is enriched with exosomes;
s4, ultracentrifugation, namely transferring the supernatant after the ultracentrifugation into an ultracentrifugation tube, and precipitating exosomes at the bottom of the ultracentrifuge tube;
S5, washing and resuspension, namely washing the precipitate by using PBS buffer solution, and resuspension in a proper amount of PBS buffer solution to obtain exosome suspension.
The extraction equipment in the S2 comprises a frame body 1, wherein the top of the frame body 1 is fixedly connected with a shell 2, the top of the shell 2 is fixedly connected with a feed inlet 3, the top of the shell 2 is fixedly connected with a motor 4, the output end of the motor 4 penetrates through the shell 2, and the inside of the shell 2 is fixedly connected with a cooling pipe 7;
screen assemblies 5, screen assemblies 5 being fixedly mounted inside housing 2;
the filter assembly 6 is fixedly arranged in the shell 2, and the filter assembly 6 is positioned below the screening assembly 5;
the filtering component 6 comprises a rotating shaft 61, the rotating shaft 61 is fixedly connected with the output end of the motor 4, the rotating shaft 61 is rotationally connected with the shell 2, the outer side of the rotating shaft 61 is rotationally connected with a fixed ring 62, and the outer side of the fixed ring 62 is fixedly connected with an intermediate component 63;
The middle component 63 comprises two filter screens 631, the two filter screens 631 are arranged symmetrically up and down, the preliminarily filtered sample flows to the edge of the filter screen 631 through the guide plate 64, then the sample flows towards the middle along the filter screen 631 under the action of the gravity of the sample, and the wavy filter screen 631 is arranged, so that the sample can have more contact area and more reasonable flow path on the filter screen 631, and the filtering process is faster. Further, more liquid parts can be filtered in a short time, filtering time is reduced, the efficiency of the whole exosome extraction process is improved, the filter screen 631 is in wave arrangement, the filter screen 631 is close to one side of the fixed ring 62, the filter screen 632 is arranged in a concave part of the filter screen 631, the through hole 635 is formed in the top of the filter screen 631, the diameter of the hole on the filter screen 631 is gradually increased from outside to inside, very fine impurities can be removed at the initial stage of filtering through the design that the hole is gradually increased, the purity of filtrate is ensured, then, in the subsequent filtering process, the passing of exosome is not blocked due to the fact that the filter hole is too small, the recovery rate of the exosome is improved, impurities with different sizes can be intercepted, the exosome can pass through smoothly, a better filtering effect is achieved on complex plant samples, meanwhile, the samples flow downwards in the process, the hole is gradually increased, the blocking risk is further reduced, the through hole 635 is arranged at the convex part of the filter screen 631, and the filter screen 632 is in elliptical.
The inner chamber of casing 2 is provided with fixed block 65, the inclined plane that outside to middle part slope is established to the surface of fixed block 65, the tip fixedly connected with spacing ring 611 of fixed block 65, spacing ring 611 and the inside fixedly connected with of casing 2, the outside sliding connection of fixed block 65 has sliding ring 610, cooling tube 7 is located the interval department of sliding ring 610 and casing 2, the top fixedly connected with guide plate 64 of sliding ring 610, guide plate 64 is down hopper-shaped, round hole 69 has been seted up to the surface that fixed block 65 is close to casing 2, round hole 69 runs through fixed block 65, spout 68 has been seted up at the outside middle part of fixed block 65, spout 68 is trapezoidal setting, the inside sliding connection of spout 68 has stopper 66, stopper 66 runs through casing 2, and stopper 66 and casing 2 sliding connection, the one end fixedly connected with locating component 67 of casing 2 is kept away from to stopper 66, locating component 67 runs through fixed block 65, locating component 67 and fixed block 65 sliding connection.
In the second embodiment, as shown in fig. 5 to 7, on the basis of the first embodiment, a fixed frame 638 is fixedly connected to one end of the filter screen 631 away from the fixed ring 62, a round rod 633 is fixedly connected to the bottom of the fixed frame 638, the round rod 633 is located inside the round hole 69, the round rod 633 is slidably connected to the fixed block 65 through the round hole 69, a spring 634 is fixedly connected to one side of the fixed frame 638 close to the round rod 633, the preliminarily filtered sample falls onto the guide plate 64 through the filter cartridge 51, falls onto the filter screen 631 along the guide plate 64, the guide plate 64 is forced to drive the slip ring 610 to move downwards under the action of gravity of the sample itself, at this time, the slip ring 610 contacts with the top of the limiting block 66 and presses the top of the limiting block 66, so that the limiting block 66 pushes the round rod 633 to move away from the limiting block 66, at this time, the inclination angle of the filter screen 631 becomes larger, the filtering speed can be increased, blocking is avoided, one end of the fixed frame 638 away from the fixed block 638 is fixedly connected with the fixed block 65, one end of the fixed frame 638 close to the filter screen 631 is fixedly connected to the baffle 636, one end of the baffle 636 away from the baffle 637 is fixedly connected to the baffle 637, and the baffle 636 is located on the same vertical plane as the baffle 637.
In the third embodiment, as shown in fig. 8 to 10, based on the first and second embodiments, the housing 2 includes a casing 21, the outer side of the casing 21 is fixedly connected with the frame 1, the inner wall of the casing 21, which is close to the screen assembly 5, is fixedly connected with a sloping plate 22, the sloping plate 22 is fixedly connected with the screen assembly 5, and a discharge hole 23 is formed in the middle of the bottom of the casing 21.
The positioning assembly 67 comprises an extension rod 671, the extension rod 671 is fixedly connected with one end, far away from the shell 21, of the limiting block 66, a preliminarily filtered sample falls above the guide plate 64 through the filter cartridge 51, falls above the filter screen 631 along the guide plate 64, the guide plate 64 is stressed to drive the sliding ring 610 to move downwards under the action of gravity of the sample, at the moment, the sliding ring 610 contacts with the top of the limiting block 66 and extrudes, the limiting block 66 pushes the trapezoid block 672 to contact with the outer side of the rotating shaft 61 in the middle through the extension rod 671, friction force between the trapezoid block 672 and the rotating shaft 61 is increased, the rotating speed of the rotating shaft 61 is reduced, the filtering assembly 5 is further slowed down, the sample entering the filter screen 631 is reduced, the sample feeding is avoided, the purity of the filtered sample is influenced, the extension rod 671 is slidingly connected with the fixing block 65, one end, far away from the limiting block 66, of the trapezoid block 672 is fixedly connected with the trapezoid block 672, and a through groove 673 is formed in one end, far away from the extension rod 671.
The screening component 5 comprises a filter cylinder 51, the filter cylinder 51 is fixedly connected with the bottom of the inclined plate 22, a rotating plate 52 is arranged in the filter cylinder 51, a crushed sample is added into the shell 21 through the feed inlet 3, the sample enters the filter cylinder 51 along the inclined plate 22, the sample can be subjected to preliminary filtration by arranging the filter cylinder 51, further, the exosome can be separated from larger cell fragments and impurities, meanwhile, the motor 4 is externally connected with a power supply, the motor 4 drives a rotating shaft 61 to rotate, the rotating shaft 61 drives the rotating plate 52 and the scraping plate 53 to rotate, so that the sample in the filter cylinder 51 is driven to rotate, then the rotating plate 52 cleans the sample on the inner wall of the filter cylinder 51, the filter cylinder 51 is prevented from being blocked, the filtering effect can be improved, meanwhile, the scraping plate 53 drives the sample at the bottom to turn upwards, the sample is prevented from being accumulated and influencing filtration, the rotating plate 52 is fixedly connected with the scraping plate 53 near the inner bottom of the filter cylinder 51, and the scraping plate 53 is obliquely arranged.
During the use, the inside of shell 21 is added through feed inlet 3 with the sample after smashing, the sample gets into the inside of straining a section of thick bamboo 51 along swash plate 22, through setting up straining a section of thick bamboo 51, and then can separate the exosome with great cell debris and impurity, motor 4 external power supply work simultaneously, motor 4 drives pivot 61 and rotates, pivot 61 drives swivel plate 52 and scraper blade 53 rotation for drive straining a section of thick bamboo 51 inside sample rotation, later swivel plate 52 clean straining a section of thick bamboo 51 sample on the inner wall, prevent straining a section of thick bamboo 51 and take place the jam.
The preliminarily filtered sample flows to the edge of the filter screen 631 through the guide plate 64, then flows towards the middle along the filter screen 631 under the action of the gravity of the sample, and the wave-shaped filter screen 631 can enable the sample to have more contact area and more reasonable flow path on the filter screen 631, so that the filtering process is faster.
The sample after preliminary filtration falls to the top of guide plate 64 through straining a section of thick bamboo 51, falls to the top of filter screen 631 along guide plate 64, under the gravity effect of sample self, guide plate 64 atress drives sliding ring 610 and moves downwards, the top contact and the extrusion of sliding ring 610 and stopper 66 produce this moment, stopper 66 promotes trapezoidal piece 672 and the outside contact of pivot 61 in middle part through extension rod 671, frictional force between trapezoidal piece 672 and the pivot 61 grow for the rotational speed of pivot 61 diminishes, and then makes screening subassembly 5 filtration slowing, makes the sample that gets into filter screen 631 reduce, avoid the sample pan feeding to be many, influence the purity of sample after filtering.
It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art and which are included in the embodiments of the present invention without the inventive step, are intended to be within the scope of the present invention. Structures, devices and methods of operation not specifically described and illustrated herein, unless otherwise indicated and limited, are implemented according to conventional means in the art.
Claims (3)
1. The extraction method of the plant exosomes is characterized by comprising the following steps:
s1, crushing plants, cleaning plant materials, and crushing plant tissues by using crushing equipment;
S2, screening and filtering, namely performing preliminary filtration on the crushed sample by using extraction equipment for removing larger impurities;
S3, centrifugal separation, namely, low-speed centrifugation, medium-speed centrifugation and high-speed centrifugation are carried out, and the supernatant fluid is enriched with exosomes;
s4, ultracentrifugation, namely transferring the supernatant after the ultracentrifugation into an ultracentrifugation tube, and precipitating exosomes at the bottom of the ultracentrifuge tube;
S5, washing and resuspension, namely washing the precipitate with PBS buffer solution, and resuspension in a proper amount of PBS buffer solution to obtain exosome suspension;
the extraction apparatus according to S2, characterized by comprising:
The cooling device comprises a frame body (1), wherein the top of the frame body (1) is fixedly connected with a shell (2), the top of the shell (2) is fixedly connected with a feed inlet (3), the top of the shell (2) is fixedly connected with a motor (4), the output end of the motor (4) penetrates through the shell (2), and the inside of the shell (2) is fixedly connected with a cooling pipe (7);
A screen assembly (5), the screen assembly (5) being fixedly mounted inside the housing (2);
A filter assembly (6), wherein the filter assembly (6) is fixedly arranged inside the shell (2), and the filter assembly (6) is positioned below the screening assembly (5);
The filtering assembly (6) comprises a rotating shaft (61), the rotating shaft (61) is fixedly connected with the output end of the motor (4), the rotating shaft (61) is rotationally connected with the shell (2), a fixed ring (62) is rotationally connected to the outer side of the rotating shaft (61), and an intermediate assembly (63) is fixedly connected to the outer side of the fixed ring (62);
The inner cavity of the shell (2) is provided with a fixed block (65), the outer surface of the fixed block (65) is provided with an inclined surface inclined towards the middle part from the outer side, the end part of the fixed block (65) is fixedly connected with a limiting ring (611), the limiting ring (611) is fixedly connected with the inside of the shell (2), the outer side of the fixed block (65) is slidably connected with a sliding ring (610), the outer surface of the fixed block (65) close to the shell (2) is provided with a round hole (69), the round hole (69) penetrates through the fixed block (65), the middle part of the outer side of the fixed block (65) is provided with a sliding groove (68), and the sliding groove (68) is in a trapezoid shape;
The inside sliding connection of spout (68) has stopper (66), stopper (66) run through casing (2), just stopper (66) and casing (2) sliding connection, the one end that keeps away from casing (2) of stopper (66) is fixedly connected with locating component (67), locating component (67) run through fixed block (65), locating component (67) and fixed block (65) sliding connection;
The middle assembly (63) comprises two filter screens (631), the two filter screens (631) are arranged symmetrically up and down, the filter screens (631) are arranged in a wave mode, a filter hole (632) is formed in one side, close to the fixed ring (62), of each filter screen (631), the filter hole (632) is located in a concave portion of the filter screen (631), a through hole (635) is formed in the top of each filter screen (631), the through holes (635) are formed in the protruding portion of each filter screen (631), and the filter holes (632) are oval;
One end of the filter screen (631) far away from the fixed ring (62) is fixedly connected with a fixed frame (638), the bottom of the fixed frame (638) is fixedly connected with a round rod (633), the round rod (633) is positioned in a round hole (69), the round rod (633) is in sliding connection with the fixed block (65) through the round hole (69), and one side of the fixed frame (638) close to the round rod (633) is fixedly connected with a spring (634);
One end of the spring (634) far away from the fixed frame (638) is fixedly connected with the fixed block (65), the top of the fixed frame (638) close to the filter screen (631) is fixedly connected with the baffle ring (636), one end of the fixed frame (638) far away from the baffle ring (636) is fixedly connected with the baffle plate (637), the baffle plate (637) and the baffle ring (636) are positioned on the same vertical plane, and the baffle plate (637) is in arc-shaped arrangement;
The screening component (5) comprises a filter cylinder (51), the filter cylinder (51) is fixedly connected with the bottom of the inclined plate (22), a rotating plate (52) is arranged in the filter cylinder (51), the rotating plate (52) is fixedly connected with a rotating shaft (61), a scraping plate (53) is fixedly connected with the bottom of the inner side of the rotating shaft (61) close to the filter cylinder (51), and the scraping plate (53) is obliquely arranged;
The positioning assembly (67) comprises an extension rod (671), the extension rod (671) is fixedly connected with one end, far away from the shell (21), of the limiting block (66), the extension rod (671) is slidably connected with the fixed block (65), one end, far away from the limiting block (66), of the extension rod (671) is fixedly connected with a trapezoid block (672), and a through groove (673) is formed in one end, far away from the extension rod (671), of the trapezoid block (672).
2. The method for extracting plant exosomes according to claim 1, wherein the cooling pipe (7) is located at a distance between the slip ring (610) and the casing (2), a guide plate (64) is fixedly connected to the top of the slip ring (610), and the guide plate (64) is in a blanking hopper shape.
3. The plant exosome extraction method according to claim 2, wherein the housing (2) comprises a shell (21), the outer side of the shell (21) is fixedly connected with the frame (1), the inner wall of the shell (21) close to the screening component (5) is fixedly connected with an inclined plate (22), the inclined plate (22) is fixedly connected with the screening component (5), and a discharging hole (23) is formed in the middle of the bottom of the shell (21).
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| CN202510196559.4A CN119701476B (en) | 2025-02-21 | 2025-02-21 | Extraction method and extraction equipment for plant exosomes |
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| CN202510196559.4A CN119701476B (en) | 2025-02-21 | 2025-02-21 | Extraction method and extraction equipment for plant exosomes |
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| CN119701476B true CN119701476B (en) | 2025-06-17 |
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| JP5366317B2 (en) * | 2009-09-09 | 2013-12-11 | 暁英 中村 | Oil strainer and oil container with oil strainer |
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| CN115197827B (en) * | 2022-07-29 | 2023-03-21 | 深圳技术大学 | A cell exosome filtration extraction device and extraction method |
| CN220812419U (en) * | 2023-06-20 | 2024-04-19 | 重庆医科大学 | Efficient exosome extraction element |
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