CN110095456B - A kind of tobacco virus detection and imaging device and using method - Google Patents
A kind of tobacco virus detection and imaging device and using method Download PDFInfo
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- CN110095456B CN110095456B CN201910292423.8A CN201910292423A CN110095456B CN 110095456 B CN110095456 B CN 110095456B CN 201910292423 A CN201910292423 A CN 201910292423A CN 110095456 B CN110095456 B CN 110095456B
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Abstract
本发明公开了一种烟草病毒检测显像装置及使用方法，包括：壳体，以及所述壳体容纳腔内设置若干个提取针；所述壳体横截面为圆环形结构，圆环形结构的所述壳体为至少两个半圆形结构连接，两个半圆形结构的所述壳体由转轴连接，所述提取针的尖端开口处设有密封膜，所述提取针的尖端贯穿所述壳体的内环壁，所述尖端朝向所述壳体的中心点，所述提取针为双层管体，所述提取针的内管一端连接所述提取针的外管一端，所述内管自由端连接活塞，所述外管容纳腔内装有检测病毒的检测剂；在使用检测显像装置时，将所述壳体套入固定在烟草茎秆上；所述提取针的尖端插入烟草茎秆外皮内部；烟草汁液进入所述内管，由所述内管的自由端进入所述外管容纳腔内，与检测病毒的检测剂混合，透过所述壳体观察检测剂的变化。
The invention discloses a tobacco virus detection and imaging device and a method for using it, comprising: a casing, and a plurality of extraction needles are arranged in the casing accommodating cavity; The casing of the structure is connected by at least two semicircular structures, the casings of the two semicircular structures are connected by a rotating shaft, the opening of the tip of the extraction needle is provided with a sealing film, and the tip of the extraction needle is Passing through the inner ring wall of the casing, the tip is facing the center point of the casing, the extraction needle is a double-layered tube body, and one end of the inner tube of the extraction needle is connected to one end of the outer tube of the extraction needle, The free end of the inner tube is connected to the piston, and the outer tube accommodating cavity is equipped with a detection agent for detecting viruses; when the detection and imaging device is used, the casing is sheathed and fixed on the tobacco stem; The tip is inserted into the outer skin of the tobacco stem; the tobacco juice enters the inner tube, and the free end of the inner tube enters the outer tube accommodating cavity, where it is mixed with the detection agent for virus detection, and the detection agent is observed through the shell The change.
Description
Technical Field
The invention relates to a plant germ prevention and control technology, in particular to a tobacco virus detection and development device and a use method thereof.
Background
The tobacco virus disease is a large disease which is most widely distributed and commonly generated in the tobacco production at present. At present, 16 tobacco virus diseases are found in China, and the viruses causing tobacco mosaic disease mainly comprise 3 viruses such as TMV, CMV, PVY and the like. In most areas, the virus diseases are mixed and occur by tobacco Cucumber Mosaic Virus (CMV), tobacco common mosaic virus (TMV) and the like, and are repeatedly infected. The field disease rate is generally 20-40%, and the weight is 40-80% (some local plots are as high as 100%). After tobacco is infected with virus, chlorophyll is damaged, photosynthesis is weakened, leaf growth is inhibited, leaves are small and deformed, and yield reduction amplitude can reach 20% -80%. After viral diseases occur, the quality of tobacco leaves is also seriously influenced, so that the quality is deteriorated.
The tobacco virus can cause large-area tobacco infection due to untimely discovery, so that the yield of tobacco is reduced, the tobacco quality is reduced, the detection result of the tobacco virus in the initial stage of the virus cannot be realized at present, the optimal prevention and treatment time is missed, the detection cannot be carried out at any time in the plant growth process, and the monitoring cannot be carried out at any time.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides a tobacco virus detection and development device and a use method thereof.
In order to achieve the purpose, the first technical scheme adopted by the invention is as follows: a tobacco virus detection and visualization device comprises: the casing, and the casing holds the intracavity and sets up a plurality of and draws needle, its characterized in that, the casing cross section is ring shape structure, ring shape structure the casing is two at least semi-circular structural connection, two semi-circular structure the casing is connected by the pivot, the most advanced opening part of drawing the needle is equipped with the seal membrane, the most advanced of drawing the needle runs through the interior rampart of casing, most advanced orientation the central point of casing, it is double-deck body to draw the needle, the inner tube one end of drawing the needle is connected the outer tube one end of drawing the needle, the piston is connected to the inner tube free end, the outer tube holds the intracavity and is equipped with the detection agent that detects the virus.
In a preferred embodiment of the present invention, the detection reagents for detecting viruses contained in the outer tube accommodating cavities of the plurality of extraction needles are different.
In a preferred embodiment of the invention, the piston is connected to a traction member.
In a preferred embodiment of the present invention, the traction member penetrates through a blunt end of the extraction needle, the cross section of the blunt end is a circular structure, the cross section of the blunt end is larger than that of the tip end, and the cross section of the tip end is a dot-shaped structure.
In a preferred embodiment of the invention, the piston is slidable within the inner tube receiving chamber.
In a preferred embodiment of the invention, the piston is in a trapezoidal structure, one end of the piston with a large cross section is connected with one end of a traction component, and a sealing ring is arranged at the blunt end of the traction component penetrating through the extraction needle.
In a preferred embodiment of the invention, at least one handle is attached to the housing.
In a preferred embodiment of the present invention, the housing is internally provided with a fixing member capable of fixing the extraction needle so as to make the extraction needle inserted into the plant more stable, and the fixing member is movably connected with the extraction needle so as to facilitate replacement of the extraction needle inside the housing.
The second technical scheme adopted by the invention is as follows: a method for using a tobacco virus detection and visualization device comprises the following steps:
(1) the shell is sleeved and fixed on the tobacco stalks;
(2) the tip of the extraction needle is inserted into the inner part of the outer skin of the tobacco stalk;
(3) tobacco juice enters the inner tube, enters the outer tube accommodating cavity from the free end of the inner tube, is mixed with a detection agent for detecting viruses, and changes of the detection agent are observed through the shell.
In a preferred embodiment of the invention, the free ends of the two housings with the semicircular structures can be sleeved to adjust the area and the perimeter of the inner ring, one end of the handle is rotatably connected with one housing with the semicircular structure, the free end of the handle can be inserted into the other housing with the semicircular structure, and the handle is connected with the two housings with the semicircular structures.
In a preferred embodiment of the present invention, the housing is made of a flexible material, can be deformed and bent, and can be attached and wound around the shape of the tobacco stalk plant to form a spiral structure wound around plants with different thicknesses.
In a preferred embodiment of the invention, the sealing membrane at the opening of the tip of the extraction needle is a slow-release membrane, the sealing membrane can be melted in tobacco stalk juice, and the melted juice in contact with the sealing membrane flows into the inner tube of the extraction needle.
In a preferred embodiment of the invention, the casing is a transparent casing, the wall of the extraction needle is a transparent wall, the traction component is pulled to separate from the inner tube, the juice of the tobacco stalks is extracted, and the transparent casing and the transparent extraction needle can observe the phenomenon that the tobacco juice extracted from the inner tube is mixed with the detection agent in the accommodating cavity of the outer tube.
In a preferred embodiment of the invention, the piston is separated from the free end of the inner tube, the air pressure in the accommodating cavity of the inner tube is smaller than the air pressure outside the extracting needle, juice in the tobacco stalks is sucked into the inner tube and enters the accommodating cavity of the outer tube from the free end of the inner tube, and the juice and the detection liquid are mixed for detection.
In a preferred embodiment of the invention, the tobacco juice is mixed with the virus detection agent to obtain healthy plants, and the plants with discoloration or precipitation are plants with diseases.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) the tobacco virus detection device can effectively detect tobacco viruses and clearly observe detection results, and has the advantages of simple structure, convenience in carrying, low production cost, simple use method, high detection speed, improvement of the prevention and treatment speed of tobacco virus diseases and prevention of large-area infection of the virus diseases.
(2) The extraction needle realizes self-absorption of plant juice by using pressure difference, the device is environment-friendly and pollution-free, and detection can be performed according to different growth time periods of tobacco.
(3) The casing can adjust the fit degree of the tobacco plants and can be made of flexible materials, scratch damage of the casing to the plant epidermis is reduced, multiple positions of the plant body can be detected (particularly along the axial direction or the length direction of the plant) in a spiral winding mode, and the plant stalks can grow vertically to play a role in fixing.
(4) The handle of the invention is convenient for carrying the detection and development device, and the shell can be fixed on the plant body when detecting the virus diseases.
(5) The shell and the extraction needle are made of transparent materials, so that the change of the mixed liquid of the tobacco juice and the virus detection agent can be observed conveniently, whether plants are infected with virus diseases can be judged quickly, and the observation is more visual.
(6) The extraction needle in the shell can be replaced, the fixing component is opened to take out the extraction needle after detection use, the extraction needle is replaced by a new extraction needle and fixed in the shell, and the extraction needle can be flexibly selected
Drawings
The invention is further explained below with reference to the figures and examples;
FIG. 1 is a perspective block diagram of a preferred embodiment of the present invention;
FIG. 2 is a perspective structural view of a preferred embodiment of the present invention;
FIG. 3 is a perspective block diagram of a preferred embodiment of the present invention;
FIG. 4 is a perspective structural view of a preferred embodiment of the present invention;
FIG. 5 is a perspective view of a preferred embodiment of the present invention;
FIG. 6 is a perspective structural view of a preferred embodiment of the present invention;
in the figure: a housing 1; extracting needle 2; a handle 3; a rotating shaft 4; an inner tube 5; a piston 6; a traction means 7.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "upper", "lower", "inner", "outer", "left", "right" and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
As shown in fig. 1-6, a tobacco virus detecting and displaying device comprises: casing 1, and casing 1 holds the intracavity and sets up a plurality of and draw needle 2, a serial communication port, 1 cross section of casing is ring annular structure, ring annular structure's casing 1 is two at least semi-circular structural connection, two semi-circular structural casing 1 is connected by pivot 4, the most advanced opening part of drawing needle 2 is equipped with the seal membrane, the most advanced interior rampart that runs through casing 1 that draws needle 2, most advanced towards the central point of casing 1, it is double-deck body to draw needle 2, 5 one end connection of inner tube that draw needle 2's outer tube one end, piston 6 is connected to 5 free ends of inner tube, the outer tube holds the intracavity and is equipped with the detection agent that detects the virus.
Specifically, the detection agents for detecting viruses in the outer tube accommodating cavities of the plurality of extraction needles 2 are different, and the detection agents can be used for detecting tobacco mosaic virus diseases, tobacco cucumber mosaic virus diseases and tobacco potato virus Y diseases.
Specifically, piston 6 connects traction element 7, and traction element 7 runs through the blunt end of drawing needle 2, and the cross section of blunt end is circular structure, and the cross section of blunt end is greater than most advanced cross section, and most advanced cross section is dot type structure, and piston 6 can hold the intracavity at inner tube 5 and slide, and piston 6 is the trapezium structure, and the one end of traction element 7 is connected to the big one end of piston 6 cross section, and traction element 7 runs through and draws the blunt end department of needle 2 and set up the sealing washer.
Specifically, connect at least one handle 3 on the casing 1, the inside fixed part that sets up of casing 1 can fix and draw needle 2, makes to draw needle 2 more firm inserting the plant, and fixed part and draw needle 2 be swing joint, can conveniently replace the inside needle 2 that draws of casing 1.
The embodiment provides a use method of a tobacco virus detection and visualization device, which comprises the following steps:
(1) the shell 1 is sleeved and fixed on the tobacco stalks;
(2) the tip of the extraction needle 2 is inserted into the inner part of the outer skin of the tobacco stalk;
(3) the tobacco juice enters the inner tube 5, enters the outer tube accommodating cavity from the free end of the inner tube 5, is mixed with the detection agent for detecting the virus, and the change of the detection agent is observed through the shell 1.
Specifically, the free ends of the two semicircular shells 1 can be sleeved, the area and the perimeter of the inner ring can be adjusted, one end of the handle 3 is rotatably connected with one semicircular shell 1, the free end of the handle 3 can be inserted into the other semicircular shell 1, and the handle 3 is connected with the two semicircular shells 1.
Specifically, casing 1 possesses flexible material composition, can produce deformation and take place the bending, can laminate the winding around the shape of tobacco stem stalk plant, and the structure winding that forms the helix is on the plant of different thicknesses.
Specifically, the seal membrane of 2 most advanced openings of extraction needle is the slowly-releasing membrane, and the seal membrane can melt in tobacco stalk juice, and the juice flows into 2 inner tubes 5 of extraction needle after the seal membrane contacts the juice and melts.
Specifically, the shell 1 is a transparent shell 1, the wall of the extraction needle 2 is a transparent wall, the traction part 7 is pulled to separate the traction part 7 from the inner tube 5, the juice of the tobacco stalks is extracted, and the transparent shell 1 and the transparent extraction needle 2 can observe the phenomenon that the tobacco juice extracted from the inner tube 5 is mixed with the detection agent in the outer tube accommodating cavity.
Specifically, the piston 6 is separated from the free end of the inner tube 5, the air pressure in the containing cavity of the inner tube 5 is smaller than the air pressure outside the extracting needle 2, the juice in the tobacco stalks is sucked into the inner tube 5 and enters the containing cavity of the outer tube from the free end of the inner tube 5, and the juice and the detection liquid are mixed for detection.
Specifically, the tobacco juice and the virus detection agent are mixed to form a healthy plant, the plant with the color change or the precipitate is a diseased plant, different virus detection agents are filled in different extraction needles 2, and the viruses such as tobacco common mosaic virus, tobacco cucumber mosaic virus, tobacco potato Y virus and the like are detected.
Specifically, the detection and development device can detect virus diseases of suspected virus plants in a laboratory, or the detection and development device is directly arranged on tobacco plant bodies, the change of the detection agent is observed during daily field management, the tips of the extraction needles 2 basically have no harm to the plant bodies, and the plants can grow normally.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (5)
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Citations (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030528A (en) * | 1987-07-13 | 1989-01-25 | 丹尼斯·W·库姆斯 | Multi-lumen epidural spinal needle |
| CN1076370A (en) * | 1992-03-19 | 1993-09-22 | 哈伯利医疗技术有限公司 | Telescoping, pharmaceutical mixing container |
| CN1134750A (en) * | 1993-11-12 | 1996-10-30 | 尤尼帕思有限公司 | Reading equipment for test strips |
| CN2247502Y (en) * | 1995-12-25 | 1997-02-19 | 王金秀 | Negative pressure type blood sampler |
| WO2001086299A3 (en) * | 2000-05-05 | 2002-05-23 | Bayer Ag | Doped nanoparticles as biolabels |
| CN1517696A (en) * | 2003-01-21 | 2004-08-04 | 拜尔健康护理有限责任公司 | A small-capacity molded waveguide optical structure |
| CN101233407A (en) * | 2005-10-28 | 2008-07-30 | 松下电器产业株式会社 | Measuring instrument, measuring device and measuring method |
| CN201184869Y (en) * | 2008-03-21 | 2009-01-21 | 林立业 | Determinator for fruit and vegetables pesticide residue |
| WO2010009735A2 (en) * | 2008-07-23 | 2010-01-28 | Dako Denmark A/S | Combinatorial analysis and repair |
| CN103115810A (en) * | 2013-02-25 | 2013-05-22 | 中国科学院植物研究所 | Method for separating apoplast juice from plant stem tissues |
| CN203310667U (en) * | 2013-09-09 | 2013-11-27 | 郑州大学 | Microneedle for detecting liquid by quickly extracting liquid in sample |
| CN203324042U (en) * | 2013-04-25 | 2013-12-04 | 安徽理工大学 | Portable confined water well type water sample collector |
| CN205071752U (en) * | 2015-09-16 | 2016-03-09 | 烟台康锐生物医药科技有限公司 | Plant transfusion device |
| CN205958303U (en) * | 2016-08-23 | 2017-02-15 | 刘一涵 | Plant specimen sampling device |
| CN107796808A (en) * | 2017-10-13 | 2018-03-13 | 窦晓鸣 | A kind of portable detector |
| CN108276215A (en) * | 2018-02-13 | 2018-07-13 | 广西中烟工业有限责任公司 | A kind of tobacco seedlings dedicated fertilizer and preparation method thereof with rooting promoting |
| CN108458897A (en) * | 2018-04-12 | 2018-08-28 | 胡琴 | A kind of petroleum transportation pipeline anticorrosive paint production technology |
| CN207987249U (en) * | 2017-12-26 | 2018-10-19 | 东莞赛尔生物科技有限公司 | Sampler for extracting sample from FFPE histotomies |
| CN208013089U (en) * | 2018-03-06 | 2018-10-26 | 大连诚泽检测有限公司 | It is a kind of from hybrid quick display device |
| CN109152892A (en) * | 2016-05-19 | 2019-01-04 | 特鲁德尔医学国际公司 | Intelligent valve holding chamber |
| CN109562373A (en) * | 2016-04-29 | 2019-04-02 | 贝比思公司 | Birth dot system and instrument, biochemical magazine and method for neonatal screening |
| CN110799137A (en) * | 2017-06-29 | 2020-02-14 | 爱惜康有限责任公司 | Trocar obturator with detachable rotating tissue fastener |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9428795B2 (en) * | 2012-02-23 | 2016-08-30 | Hitachi, Ltd. | Plant tissue sampling method and plant gene analysis method |
| US9709987B2 (en) * | 2014-07-31 | 2017-07-18 | Elwha Llc | Systems and methods for deactivating plant material outside of a growing region |
| CN109646991B (en) * | 2019-01-15 | 2021-08-03 | 山西药科职业学院 | Medicinal plant juice extractor |
| CN211061254U (en) * | 2019-11-13 | 2020-07-21 | 重庆兴宇工程建设监理有限公司 | Cement sample collection device |
-
2019
- 2019-04-12 CN CN201910292423.8A patent/CN110095456B/en active Active
Patent Citations (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030528A (en) * | 1987-07-13 | 1989-01-25 | 丹尼斯·W·库姆斯 | Multi-lumen epidural spinal needle |
| CN1076370A (en) * | 1992-03-19 | 1993-09-22 | 哈伯利医疗技术有限公司 | Telescoping, pharmaceutical mixing container |
| CN1134750A (en) * | 1993-11-12 | 1996-10-30 | 尤尼帕思有限公司 | Reading equipment for test strips |
| CN2247502Y (en) * | 1995-12-25 | 1997-02-19 | 王金秀 | Negative pressure type blood sampler |
| WO2001086299A3 (en) * | 2000-05-05 | 2002-05-23 | Bayer Ag | Doped nanoparticles as biolabels |
| CN1517696A (en) * | 2003-01-21 | 2004-08-04 | 拜尔健康护理有限责任公司 | A small-capacity molded waveguide optical structure |
| CN101233407A (en) * | 2005-10-28 | 2008-07-30 | 松下电器产业株式会社 | Measuring instrument, measuring device and measuring method |
| CN201184869Y (en) * | 2008-03-21 | 2009-01-21 | 林立业 | Determinator for fruit and vegetables pesticide residue |
| WO2010009735A2 (en) * | 2008-07-23 | 2010-01-28 | Dako Denmark A/S | Combinatorial analysis and repair |
| CN103115810A (en) * | 2013-02-25 | 2013-05-22 | 中国科学院植物研究所 | Method for separating apoplast juice from plant stem tissues |
| CN203324042U (en) * | 2013-04-25 | 2013-12-04 | 安徽理工大学 | Portable confined water well type water sample collector |
| CN203310667U (en) * | 2013-09-09 | 2013-11-27 | 郑州大学 | Microneedle for detecting liquid by quickly extracting liquid in sample |
| CN205071752U (en) * | 2015-09-16 | 2016-03-09 | 烟台康锐生物医药科技有限公司 | Plant transfusion device |
| CN109562373A (en) * | 2016-04-29 | 2019-04-02 | 贝比思公司 | Birth dot system and instrument, biochemical magazine and method for neonatal screening |
| CN109152892A (en) * | 2016-05-19 | 2019-01-04 | 特鲁德尔医学国际公司 | Intelligent valve holding chamber |
| CN205958303U (en) * | 2016-08-23 | 2017-02-15 | 刘一涵 | Plant specimen sampling device |
| CN110799137A (en) * | 2017-06-29 | 2020-02-14 | 爱惜康有限责任公司 | Trocar obturator with detachable rotating tissue fastener |
| CN107796808A (en) * | 2017-10-13 | 2018-03-13 | 窦晓鸣 | A kind of portable detector |
| CN207987249U (en) * | 2017-12-26 | 2018-10-19 | 东莞赛尔生物科技有限公司 | Sampler for extracting sample from FFPE histotomies |
| CN108276215A (en) * | 2018-02-13 | 2018-07-13 | 广西中烟工业有限责任公司 | A kind of tobacco seedlings dedicated fertilizer and preparation method thereof with rooting promoting |
| CN208013089U (en) * | 2018-03-06 | 2018-10-26 | 大连诚泽检测有限公司 | It is a kind of from hybrid quick display device |
| CN108458897A (en) * | 2018-04-12 | 2018-08-28 | 胡琴 | A kind of petroleum transportation pipeline anticorrosive paint production technology |
Non-Patent Citations (4)
| Title |
|---|
| Tobacco Mosaic Virus Infection Results in an Increase in Recombination Frequency and Resistance to Viral, Bacterial, and Fungal Pathogens in the Progeny of Infected Tobacco Plants;Palak Kathiria 等;《Plant Physiology》;20100524;第153卷(第4期);第1859-1870页 * |
| Using image analysis for quantitative assessment of needle bladder rust disease of Norway spruce;A.Ganthaler 等;《Plant Pathology》;20180211;第1122-1130页 * |
| 烟草漂浮秒烟草花叶病毒带毒率检测;刘勇;《湖南农业大学学报（自然科学版）》;20081231(第01期);第33-38页 * |
| 烟草花柱引导组织细胞程序性死亡动态研究;陈妍;《中国优秀硕士学位论文全文数据库 基础科学辑》;20170615(第6期);第1-10页 * |
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