CN110698551B

CN110698551B - Application of soybean auxin response gene or protein thereof

Info

Publication number: CN110698551B
Application number: CN201911113850.1A
Authority: CN
Inventors: 冯献忠; 杨素欣; 高金珊; 宋晓峰
Original assignee: Northeast Institute of Geography and Agroecology of CAS
Current assignee: Northeast Institute of Geography and Agroecology of CAS
Priority date: 2019-11-14
Filing date: 2019-11-14
Publication date: 2021-06-08
Anticipated expiration: 2039-11-14
Also published as: CN110698551A

Abstract

The invention relates to the technical field of genetic breeding, in particular to an application of a soybean auxin response gene or a protein thereof. The research of the invention shows that the auxin response gene or the protein thereof from the soybean can regulate and control the development of soybean pods or leaves, thereby being used for realizing the improvement of the soybean yield. By using GmSP1 as a marker, the high yield performance of plant germplasm, particularly soybean germplasm, can be identified.

Description

Application of soybean auxin response gene or protein thereof

Technical Field

The invention relates to the technical field of genetic breeding, in particular to an application of a soybean auxin response gene or a protein thereof.

Background

Soybean (Glycine max), also called soybean, is the most important bean in the world and is an important source of vegetable protein and oil. In addition, soybean is rich in carbohydrate, dietary fiber, B vitamins, minerals, soybean isoflavone, soybean lecithin, soybean sterol, etc. Since soybeans have important and special economic values, increasing the yield of soybeans is a common pursuit of breeding technicians.

The seed size is one of the most important factors influencing the crop yield, and the research on the seed size related gene is used for searching the functional gene related to high yield, so that the method has important significance for improving the crop yield. In crops, seeds are the most important organ for storing products. Starting from agriculture, seed size is one of the important criteria for crop domestication. Different plants vary widely in seed size, but the mechanism for determining seed size is not completely understood at present.

So far, there are many reports on QTL analysis of soybean yield and yield-constituting factors. Researches show that the yield traits of soybeans are comprehensive traits, the genetic basis is complex, and most yield traits of the soybeans belong to quantitative traits. For example: mian detected 7 and 9 QTLs affecting grain weight in 2 soybean populations, respectively. Mangham at F₂And

F

_2：33 and 5 markers associated with changes in grain weight were detected in the population, respectively. The Wangxian wisdom detects that the number of pods per plant, the number of grains per clamp and other 5 QTLs related to the yield traits are 43 in total. Previous studies have shown that yield-related QTLs are mainly focused on the C2 and M linkage groups, and that the hundred-grain weight-related QTLs are mainly focused on the a2, L, M, N and O linkage groups.

Soybean is one of the most widely consumed main crops, and increasing the size of soybean seeds is an important strategy for increasing the yield of soybean. However, the identification of genes that influence yield, such as soybean seed size and pod size, is not yet sufficiently studied.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide the use of a soybean auxin response gene or a protein thereof. Researches show that the corresponding gene of the soybean auxin or the protein thereof can regulate and control the sizes of pods, leaves and seeds of leguminous plants.

The invention provides an application of at least one of the following I) to V) in regulating and controlling the size of a plant pod, the size of a leaf and/or the size of a seed;

I) GmSP1 protein;

II), a protein which is obtained by substituting, deleting or adding one or more amino acids in the amino acid sequence of the GmSP1 protein and has the same or similar functions as GmSP 1;

III), nucleic acid molecules encoding the proteins of I) or II);

IV), a nucleic acid molecule which is substituted, deleted or added with one or more nucleotides in the nucleotide sequence of the nucleic acid molecule of III) and can encode the same or similar functional protein;

v), a substance capable of modulating the level or activity of at least one of I) to V).

In the invention, the amino acid sequence of the GmSP1 protein is shown as SEQ ID NO 1;

the sequence of the nucleic acid molecule for coding the protein shown in SEQ ID NO. 1 is shown in SEQ ID NO. 2.

In the present invention, the plant is a leguminous plant; the plant used for verifying the function of the GmSP1 in the invention is a leguminous plant soybean, in particular a lotus 12.

In the invention, the regulation and control of the size of the pod comprises the regulation and control of the length, the weight and the space of the pod skin of the pod;

the regulation of the size of the leaf comprises regulation of the number of cells in the leaf;

the regulation and control of the seed size comprises regulation and control of the seed shape and the seed weight.

In the invention, the regulation comprises two aspects of positive regulation or negative regulation. The glyma.02gd142500 gene of the Gmsp1 mutant phenotype, which is mutated at exon G to a, resulting in a substitution of amino acids G to E. The study on the pod and seed of the Gmsp1 mutant compared with the wild type shows that the study shows that the pod length of the Gmsp1 gene mutant is reduced compared with the pod length of the wild type lotus bean 12, and the hundred grain weight of the seed is reduced by 30.52 percent compared with the lotus bean 12. The Gmsp1 mutant and the lotus 12 had smaller pod weight, grain weight and pod weight than the wild type, but the ratio of grain weight to pod weight was larger than the wild type. Moreover, the leaf size of the Gmsp1 mutant was smaller than that of lotus 12. The above results indicate that overexpression of the GmSP1 gene, promotion of the transcription level of the GmSP1 gene, increase of the protein level of the GmSP1 protein or promotion of the activity of the GmSP1 protein are beneficial to increase of pod length, increase of pod weight, enlargement of pod space, increase of leaf cell number, improvement of seed shape and increase of seed weight. Knocking out or knocking down the GmSP1 gene, inhibiting the transcription level of the GmSP1 gene, reducing the level of the GmSP1 protein or inhibiting the activity of the GmSP1 protein can reduce the length of a pod, reduce the weight of the pod, reduce the space of pod skin, reduce the number of leaf cells, cause the flattening of seeds and reduce the weight of the seeds. In the embodiment of the invention, the functional deletion of the Glyma.02G142500 gene in the Gmsp1 mutant causes the length of a pod to be reduced, the weight of the pod to be reduced, the space of the pod skin to be reduced, the number of leaf cells to be reduced, the seeds to be flattened and the weight of the seeds to be reduced.

The invention also provides a preparation for increasing the size of a fruit pod, the size of a leaf and/or the size of a seed, which is characterized by comprising at least one of the following i) to v):

i) a GmSP1 protein or a nucleic acid molecule encoding a GmSP1 protein;

ii) an expression vector comprising a nucleic acid encoding a GmSP1 protein;

iii) a recombinant host comprising ii);

iv) a promoter or enhancer that enhances expression of the GmSP1 gene;

v), an inducer promoting expression of GmSP1 gene;

vi) agents that increase the activity of GmSP1 protein.

Increasing pod length, increasing pod weight, expanding pod skin space can avoid the seeds from abutting each other due to space limitations, thereby avoiding shape changes of the seeds due to extrusion, and improving seed size. The increase of the number of leaf cells and the enlargement of the leaf area are beneficial to the accumulation of nutrient substances generated by photosynthesis, thereby improving the seed weight. Thus, formulations that increase pod size, leaf size, and/or seed size are beneficial for increasing plant yield.

In the preparation, the amino acid sequence of the GmSP1 protein is shown as SEQ ID NO. 1. The nucleotide sequence of the nucleic acid molecule for coding the GmSP1 protein is shown as SEQ ID NO. 2.

The preparation of the invention is applied to improving the yield of crops.

The invention also provides a method for improving the yield of crops, which improves the level and/or activity of endogenous GmSP1 protein of the crops or enables the crops without GmSP1 or GmSP1 inactivation to express the GmSP1 protein by using the preparation disclosed by the invention.

In the invention, the improvement of the crop yield refers to the breeding improvement of the existing crops, so as to obtain a new variety with higher yield. Or applying an inducer capable of promoting the expression of GmSP1 gene or an agent capable of improving the activity of GmSP1 protein to crops so as to improve the yield of plants.

In some embodiments, a method of inactivating GmSP1 in a crop expressing GmSP1 protein comprises:

constructing a vector containing a nucleic acid encoding GmSP1 protein, and transforming into agrobacterium; infecting the seed or explant of the crop with said agrobacterium.

Moreover, GmSP1 can also be used as a marker for screening high-yield crops in plant breeding.

The present invention also provides a formulation for screening high-yielding crops, which comprises:

agents that detect transcription levels of the GmSP1 gene;

and/or detecting the expression level or active agent of GmSP1 protein.

The preparation provided by the invention is applied to screening of high-yield crops.

The invention also provides a method for screening high-yield crops, which detects the transcription level of the GmSP1 gene of the crops or detects the expression level or activity of the GmSP1 protein by using the preparation.

The assay of the present invention includes the measurement of the expression level or activity level. For example, Western blot is adopted for detecting the expression level of the GmSP1 protein; the transcription level of the GmSP1 gene is detected by adopting a real-time PCR mode. By using the identification method, the prediction of whether the germplasm is high in yield can be realized only by detecting the tender tissues of the crops, and the breeding period is shortened.

The research of the invention shows that the auxin response gene or the protein thereof from the soybean can regulate and control the development of soybean pods or leaves, thereby being used for realizing the improvement of the soybean yield. By using GmSP1 as a marker, the high yield performance of plant germplasm, particularly soybean germplasm, can be identified.

Drawings

FIG. 1 shows the genomic sequence of the soybean GmSP1 gene of the present invention, in which the exon sequences are shown in bold, the intron sequences are shown in italics,

indicating start-upIn the case of a hybrid vehicle,

represents a terminator;

FIG. 2 shows that soybean Gmsp1 mutant has significantly smaller pods and seeds and significantly reduced weight per hundred grains, wherein A is three pods at the R9 stage, B is the sizes of wild-type and Gmsp1 mutant seeds, C is the positive and negative cross results of wild-type lotus bean 12 and Gmsp1 mutant, D is the pod length analysis (NSPP, number of single pod; DAF, days after flowering) of wild-type lotus bean 12 and Gmsp1 mutant after flowering, and E is the weight per hundred grains of wild-type and Gmsp1 mutant;

fig. 3 shows that the reduction of pods from Gmsp1 mutant resulted in smaller seeds, where a is pod and seed at different developmental stages, dashed white line represents the inner boundary of the seed, single-, double-and triple-pod R6 projection areas from the mutants of B lotus bean 12 and Gmsp1, n is 20, C is the ratio of the skin to the weight of lotus bean 12 of Gmsp1 mutant to the skin to the weight of the seed, n is 20, D is the comparison of the weight of the Gmsp1 mutant to the pod, skin and seed of lotus bean 12 (stage R6), n is 20;

fig. 4 shows that the leaf size of the Gmsp1 mutant is significantly smaller than that of the wild type lotus 12, wherein a is the seventh leaf of the lotus 12 and Gmsp1 mutant V9, B is the epidermal cell of the seventh leaf of the lotus 12 and Gmsp1 mutant scanned by an electron microscope, C is the area statistic of the seventh leaf of the lotus 12 and Gmsp1 mutant, n is 10, D is the size statistic of the seventh leaf of the lotus 12, lotus 12 and Gmsp1 mutant, and n is greater than 50.

Detailed Description

The invention provides the application of the soybean auxin response gene or the protein thereof, and a person skilled in the art can use the content for reference and appropriately improve the process parameters to realize the purpose. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.

The test materials adopted by the invention are all common commercial products and can be purchased in the market.

The nucleic acid molecule encoding the GmSP1 protein comprises genomic DNA, cDNA, recombinant DNA or mRNA, hnRNA encoding the GmSP1 protein; or a nucleic acid molecule which is reverse complementary to the above DNA, cDNA, recombinant DNA or mRNA.

The nucleic acid molecule can be modified or optimized according to actual needs, so that the gene expression is more efficient; for example, the codon of the GmSP1 gene of the present invention may be changed to conform to the preference of a recipient plant while maintaining the amino acid sequence thereof, according to the preference of the recipient plant. ② or modifying the gene sequence adjacent to the initiating methionine to allow efficient initiation of translation; for example, the modification is carried out using a sequence known to be effective in plants. Connecting with various plant expression promoters to facilitate the expression of the promoter in plants; such promoters may include constitutive, inducible, time-regulated, developmentally regulated, chemically regulated, tissue-preferred, and tissue-specific promoters; the choice of promoter will vary with the time and space requirements of expression, and will also depend on the target species; (iv) introduction of enhancer sequences such as intron sequences (e.g.from Adhl and bronzel) and viral leader sequences (e.g.from TMV, MCMV and AMV).

In the present invention, the vector may be a plasmid, a cosmid, a phage, or a viral vector. The host may be a fungus, a bacterium, an alga or a cell. The bacterium is preferably an Agrobacterium.

For plants not containing GmSP1, the gene fragment of GmSP1 may be introduced into plant cells by chemical methods, shotgun methods, microinjection, electroporation, or the like, or the gene fragment of GmSP1 may be introduced into plant cells by homologous recombination, zinc finger nucleases, TALENs, CRISPRs, or the like.

The invention is further illustrated by the following examples:

example 1 isolation and structural analysis of the GmSP1 Gene in Soybean

(1) Isolation of genes

mRNA was isolated from young leaves of soybean variety Williams82, and first strand cDNA was synthesized using the mRNA as a template and oligo (T)17 as a primer. Then, PCR amplification was carried out using the first cDNA strand as a template and primers (5'-ATGGTGTTTGAGGAAACTGAGC-3') and (5'-GAAGTGGTAACCATTGATT-3') to obtain 1 cDNA fragment 513bp in length of the GmSP1 gene, which was cloned into a pGEM T Easy vector (TaKaRa Co.) and named pGEM T Easy-GmSP 1.

The GmEM T Easy-GmSP1 contains GmSP1 CDS sequence shown in SEQ ID NO:2, which is 513bp in total and encodes a 170 amino acid protein (SEQ ID NO: 1).

(2) Structural analysis of genes

The DNA of the young leaf of Williams82 is extracted as a material, and then the genome DNA is used as a template to amplify to obtain a fragment of GmSP1 genome, wherein the sequence of the GmSP1 DNA is shown as SEQ ID NO:3 and is 1741bp in total, and the GmSP1 DNA fragment contains 1 intron and 2 exons (shown as figure 1).

Example 2 the Soybean GmSP1 Gene regulates pod and seed size

10000 grains of lotus 12, a soybean cultivar widely planted in Huanghuaihai areas, are mutagenized by gamma rays of 60Co, 1298 mutants of 900 strains are identified by the M2 mutant, mutant materials with smaller pods are obtained by screening, and Gmsp1 mutants with stable phenotypes are obtained after 4 generations of planting.

The Gmsp1 mutant is hybridized with a distant variety Williams82 to construct a map-based clone population, and the Glyma.02G142500 gene which controls the phenotype of the Gmsp1 mutant and causes the substitution of amino acids G to E by the mutation of G to A at an exon is successfully identified by using map-based cloning.

The pods and seeds of the Gmsp1 mutant are smaller than those of the wild type at each stage of development, the length difference of the Gmsp1 mutant from the single-pod, the two-pod and the three-pod of the lotus 12 is not large at 6 days after flowering, the length difference of the pods is increased sharply at 16 days after flowering, the degree of the Gmsp1 mutant from the single-pod, the two-pod and the three-pod of the lotus 12 is not increased any more after 28 days of flowering, and the length of the pods of the Gmsp1 mutant is only 55.8% of the length of the lotus 12. Statistical analysis of the weight of the dried seeds of the Gmsp1 mutant revealed that the weight of the Gmsp1 mutant (17.57 +/-0.42 g) was reduced by 30.52% compared with the weight of lotus bean 12(25.29 +/-0.55 g) (see FIG. 2).

Example 3 the Soybean GmSP1 Gene regulates seed size by regulating pod size

At the pod development stage (R3-R4), the seeds of the Gmsp1 mutant are basically the same as the seeds of the wild type, but the pods are very different (as shown in figure 2); in the period of soybean development from R5 to R6, the seeds of the wild lotus 12 still have enough space in the fruit pod and do not contact with each other; the seeds of the Gmsp1 mutant are close to each other due to space limitation, so that the shape of the seeds is changed (the contact part between the seeds is flat, and the wild type is an ellipsoid). Statistical analysis revealed that the Gmsp1 mutant and lotus 12 all had smaller pod weight, grain weight and pod weight than the wild type, but the ratio of grain weight to pod weight was larger than the wild type (see fig. 3). This result suggests that the reduced size of the Gmsp1 mutant seeds may be due to insufficient pod space limiting the normal development of the seeds.

Example 4 the Soybean GmSP1 Gene regulates leaf size

In addition to the smaller pods and seeds, the Gmsp1 mutant also had altered leaf size. By statistically analyzing the leaf area of the third mature leaf at the seventh maturing stage V9, we found that the leaf area of the Gmsp1 mutant (57.29 + -5.08 cm2) was reduced by 52.56% compared with the lotus bean 12(27.17 + -2.75 cm 2). Scanning electron microscope observation of epithelial cells of the corresponding sites of the seventh mature triple leaves of the lotus bean 12 and the Gmsp1 mutant shows that the mutant has no obvious difference with the wild type in terms of cell shape and size (FIG. 4). The above results indicate that the reduced leaf size of the Gmsp1 mutant is caused by the change in cell number, not cell size.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that it is obvious to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.

Sequence listing

<110> institute of geography and agroecology of northeast China academy of sciences

<120> application of soybean auxin response gene or protein thereof

<130> MP1926533

<160> 3

<170> SIPOSequenceListing 1.0

<210> 1

<211> 170

<212> PRT

<213> Soybean (Glycine max)

<400> 1

Met Val Phe Glu Glu Thr Glu Leu Arg Leu Gly Leu Gly Leu Cys Leu

1 5 10 15

Pro Gly Asn Gly Thr Thr Ala Thr Thr Glu Ala Ala Ala Ala Glu Leu

20 25 30

Gly Val Arg Lys Arg Gly Phe Ser Glu Thr Glu Thr Asp Glu Thr Ala

35 40 45

Thr Val Asp Leu Met Leu Asn Leu Ser Pro Lys Glu Ala Ala Ala Ala

50 55 60

Asp Gly Ala Asp Pro Arg Glu Lys Pro Lys Thr Ser Pro Lys Glu Lys

65 70 75 80

Thr Leu Leu Leu Pro Asp Pro Ala Lys Pro Pro Ala Lys Ala Gln Val

85 90 95

Val Gly Trp Pro Pro Val Arg Ser Phe Arg Lys Asn Met Phe Ala Ala

100 105 110

Gln Lys Ser Ser Gly Gly Glu Glu Ser Glu Lys Ser Ser Pro Asn Ala

115 120 125

Ser Phe Val Lys Val Ser Met Asp Gly Ala Pro Tyr Leu Arg Lys Val

130 135 140

Asp Leu Lys Met Tyr Lys Ser Tyr Pro Glu Leu Ser Asp Ala Leu Gly

145 150 155 160

Lys Met Phe Ser Ser Phe Thr Ile Gly Asn

165 170

<210> 2

<211> 513

<212> DNA

<213> Soybean (Glycine max)

<400> 2

atggtgtttg aggaaactga gctgaggctg ggactgggac tatgtctccc tggaaatgga 60

accacggcaa caactgaagc tgctgctgcg gaattaggag tgaggaagag agggttctct 120

gagactgaaa ctgatgaaac agccaccgtt gatttgatgc ttaacctctc acccaaggaa 180

gctgctgctg ctgatggtgc agatccacgt gagaagccaa agacttcgcc gaaggagaag 240

acccttctgc ttcccgatcc cgccaagcct cctgccaagg cgcaagtggt gggatggcca 300

cccgtgaggt ctttccggaa gaacatgttc gcagcccaaa agagcagcgg cggagaggaa 360

agcgaaaaga gcagccctaa tgcaagcttt gtcaaagtta gcatggatgg agcaccttac 420

ctccgcaaag ttgacttgaa gatgtacaag agttacccag agctctctga tgccttgggc 480

aaaatgttta gctccttcac cattggtaac taa 513

<210> 3

<211> 1741

<212> DNA

<213> Soybean (Glycine max)

<400> 3

ggctccccac aacgtaataa cgtgcctgcc cctatacctc accccactta ctattattat 60

tgtacaacac aaccaggtcc ctgaatgctt tttagagaga gaaagagtaa taataataaa 120

gtggaagaaa aagaattagg agagtgaaac aagaagaaga agaagaagaa gaagaagaag 180

aagaattgag aagatggtgt ttgaggaaac tgagctgagg ctgggactgg gactatgtct 240

ccctggaaat ggaaccacgg caacaactga agctgctgct gcggaattag gagtgaggaa 300

gagagggttc tctgagactg aaactgatga aacagccacc gttgatttga tgcttaacct 360

ctcacccaag gaagctgctg ctgctgatgg tgcagatcca cgtgagaagc caaagacttc 420

gccgaaggag aagacccttc tgcttcccga tcccgccaag cctcctgcca agtaagcact 480

agtttaaaat aattgaatat actagcatac aaaaaatcaa tagaccaaac ttagtgacgg 540

aaattatgtt tttcgtcact aattctgaag ttacaaaatt tctagtcgca tttttttaaa 600

gaaaattatt acatataaat cattttttca ctaattttat tttttcttgc atatgcatgt 660

gtaaaattga aagaagggga cttgttctgg taatttttct atttgtaact tgatcttgca 720

tggaaataaa catatacata taatattgtg cttatattta tgatatggct aatttgaggt 780

gcccgaatgg gaaacagtta gaaacgaaag ccaaaatgac agatcatgaa aaataaagaa 840

gaaattaatg acactgactg aaaaatagat tcgtgagaga gagaagcaag aaaaattaaa 900

actctagatt ccttcagctc tcggatacct tttttgcaag ctagaaatac gatctgcatg 960

ccttcaaata gtttgggtag ctagttttgc atgtacgggt aataatacca atagccttac 1020

atatctactt gcgaattcta taagccatga tgatatgtat ctgctggtgt gaaatcttat 1080

ttattttggt gccaacagta tcatgtatgt atatcttctt ttgcagactg ttaattagca 1140

tgctgatgaa tgaaacaatt aattaagaaa atccatatat atatatatat atatgcttat 1200

ctatttgtat atgtatagaa aagagagaat tggattaaca tggccataaa cagaaaccaa 1260

attttccata ttctcagtct tgatgtgcag ccaagttaat tatgaatttt aaggctaatc 1320

aactagttct tcaaggaaac tgactatata tatatatata tatatatata tatatatata 1380

tatatatata tatgattaat tgatttaatt ttagtttcat tatatgcata attccatccc 1440

caacccctct aactatgaag tgattatacg aatattaatg aatcaaaaca aaatgaacac 1500

tttagggcgc aagtggtggg atggccaccc gtgaggtctt tccggaagaa catgttcgca 1560

gcccaaaaga gcagcggcgg agaggaaagc gaaaagagca gccctaatgc aagctttgtc 1620

aaagttagca tggatggagc accttacctc cgcaaagttg acttgaagat gtacaagagt 1680

tacccagagc tctctgatgc cttgggcaaa atgtttagct ccttcaccat tggtaactaa 1740

t 1741

Claims

Use of GmSP1 protein or a nucleic acid encoding the same for regulating soybean pod size, leaf size and/or seed size; the amino acid sequence of the GmSP1 protein is shown as SEQ ID NO. 1; the sequence of the nucleic acid molecule for coding the protein shown in SEQ ID NO. 1 is shown in SEQ ID NO. 2.
2. The use according to claim 1,

the regulation and control of the size of the fruit pod comprises regulation and control of the length of the fruit pod, the weight of the fruit pod and the space of the pod skin;

the regulation of the size of the leaf comprises regulation of the number of cells in the leaf;

the regulation and control of the seed size comprises regulation and control of the seed shape and the seed weight.
The use of GmSP1 protein or its encoding nucleic acid for increasing crop yield; the amino acid sequence of the GmSP1 protein is shown as SEQ ID NO. 1; the sequence of the nucleic acid molecule for coding the protein shown in SEQ ID NO. 1 is shown in SEQ ID NO. 2.
4. The method for improving the yield of the crops is characterized by improving the level and/or activity of endogenous GmSP1 protein of the crops, or enabling the crops without GmSP1 or GmSP1 inactivation to express the GmSP1 protein, wherein the amino acid sequence of the GmSP1 protein is shown as SEQ ID NO: 1.
The application of GmSP1 protein or coding nucleic acid thereof in screening high-yield crops; the amino acid sequence of the GmSP1 protein is shown as SEQ ID NO. 1; the sequence of the nucleic acid molecule for coding the protein shown in SEQ ID NO. 1 is shown in SEQ ID NO. 2.
6. A method for screening high-yield crops is characterized in that the crops are detectedGmSP1The gene transcription level or the detection of the expression level or the activity of GmSP1 protein, wherein the amino acid sequence of the GmSP1 protein is shown as SEQ ID NO:1GmSP1The nucleic acid sequence of the gene is shown as SEQ ID NO. 2.