CN110883001B - Analyzer magnetic bead cleaning device - Google Patents

Abstract

The present invention relates to an analyzer magnetic bead cleaning device, comprising a base assembly, a mixing assembly, a rotating assembly, a washing liquid filling assembly and a waste liquid suction assembly; the base assembly comprises a bottom plate, a bearing seat, a rotating base shaft, a rotating motor and a mixing lifting guide shaft; the mixing assembly comprises a mixing disk, a mixing position, a mixing screw motor and a mixing rotating motor; the rotating assembly comprises a rotating disk and an eccentric rotating cup holder; the washing liquid filling assembly comprises a filling fixed plate, a washing liquid needle, a washing liquid filling bracket, a substrate needle, a liquid injection needle rack, a pillar and a magnet rack; the waste liquid suction assembly comprises a linear guide rail, a supporting vertical plate, a guide rail adapter rack, a suction screw motor, a reinforcing rib, a waste liquid suction bracket and a plurality of waste liquid suction needle units. The analyzer magnetic bead cleaning device uses multiple needles to fill washing liquid, multiple needles to absorb waste liquid and multiple positions of eccentric rotation mixing, and has good cleaning effect, high instrument throughput, low cost and low failure rate.

Description

Analyzer magnetic bead cleaning device

Technical Field

The invention relates to a magnetic bead cleaning device for an analyzer, belonging to the technical field of medical equipment.

Background Art

Chemiluminescence immunoassay (CLIA) is a method for quantitative immunological analysis of patient body fluid samples. It is used to test the detection and analysis of various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. It has the advantages of high sensitivity, wide linear range, simple equipment, convenient operation, fast detection speed, process automation, etc., which can greatly improve the efficiency of immunoassay, so it is widely used in modern clinical immunoassay diagnosis and life optional research. Combine the highly sensitive chemiluminescence measurement technology with highly specific immune response. It is usually necessary to coat the specific biological raw materials on magnetic beads, and then add a medium that reacts with it to emit light after a series of immune reactions with antigens or antibodies and washing. The luminescence intensity is collected by a specific optical measuring instrument, and the detection object is quantitatively analyzed according to the strength of the luminescence signal. The magnetic bead cleaning device in the reaction cup mainly cleans the unreacted excess reagents in the reaction cup to ensure the accuracy of the results.

The magnetic bead cleaning device of the analyzer is a device invented to achieve continuous measurement without interrupting the process. The reaction cup is cleaned multiple times on a rotating disc to wash away excess reagents that have not participated in the reaction. However, ordinary cleaning devices generally cannot achieve continuous operation of reaction cup cleaning, precise filling of washing solution, eccentric mixing and rotation, magnetic bead adsorption, waste liquid suction, substrate filling and other functions, thereby reducing the cleaning effect and operating efficiency. The characteristic functions of good cleaning effect, high instrument throughput, less execution power source, low cost and low failure rate are achieved.

Summary of the invention

In view of the deficiencies in the prior art, the present invention provides a magnetic bead cleaning device for an analyzer. The magnetic bead cleaning device for an analyzer has good cleaning effect, high instrument throughput, low cost and low failure rate.

The technical solution of the present invention to solve the above technical problems is as follows: an analyzer magnetic bead cleaning device, the analyzer magnetic bead cleaning device comprises, from bottom to top, a base component, a mixing component, a rotating component, a washing liquid filling component and a waste liquid suction component;

The base assembly includes a base plate, a bearing seat, a rotating base shaft, a rotating motor and a mixing and lifting guide shaft, the upper end of the bearing seat is fixedly connected to the base plate, the lower end of the rotating base shaft passes through the base plate, the lower end of the rotating base shaft is connected to the bearing seat through a bearing, the rotating motor is fixedly connected to the lower end of the base plate, the shaft of the rotating motor passes through the base plate, a driving pulley is provided on the shaft of the rotating motor, a driven pulley is provided on the rotating base shaft, the driving wheel and the driven pulley are connected through a synchronous belt, and the lower end of the mixing and lifting guide shaft is fixedly connected to the base plate through a guide shaft support;

The mixing component includes a mixing disk, a mixing position, a mixing screw motor and a mixing rotary motor. The mixing disk is provided with a plurality of mixing positions. The mixing position is rotatably connected to the mixing disk through a bearing. The lower end of the mixing disk is fixedly connected to a motor bracket. The lower end of the motor bracket is fixedly connected to the mixing rotary motor. The shaft of the mixing motor passes through the upper end of the motor bracket. The upper end of the shaft of the mixing motor is provided with a driving pulley, and the driving pulley is connected to the mixing position through a synchronous belt. The lower end of the mixing disk is provided with a screw nut. The rod motor is fixedly connected to the lower end of the base plate, the shaft of the mixing screw motor passes through the base plate, and the screw of the mixing screw motor is connected to the screw nut by a thread; the lower end of the mixing disk is fixedly connected with a mixing lifting linear bearing, the upper end of the mixing lifting guide shaft is slidably connected with the mixing lifting linear bearing, and the lower end of the mixing disk is fixedly connected with a lifting sensor contact piece; the rotation of the mixing screw motor can drive the mixing disk to rise and fall, and the mixing lifting guide shaft and the mixing lifting linear bearing play a guiding role and a limiting role, so that the mixing disk can be lifted and lowered more smoothly;

The rotating assembly comprises a rotating disk and an eccentric rotating cup holder, the rotating base shaft passes through the mixing assembly, the rotating base shaft is fixedly connected to the rotating disk, a plurality of eccentric rotating cup holders are arranged at the upper end of the rotating disk, the eccentric rotating cup holders are slidably matched with the rotating disk, and a hole is arranged at the lower end of the eccentric rotating cup holder to match with the upper end of the mixing position;

The washing liquid filling assembly comprises a filling fixed plate, a washing liquid needle, a washing liquid filling bracket, a substrate needle, a liquid filling needle rack, a pillar and a magnet rack, the lower end of the washing liquid filling bracket is fixedly connected to the bottom plate, the upper end of the washing liquid filling bracket is fixedly connected to the filling fixed plate, the upper end of the filling fixed plate is fixedly connected to a plurality of liquid filling needle racks, the substrate needle is plugged into the liquid filling needle rack, and a plurality of washing liquid needles are plugged into the liquid filling needle rack, the upper end of the pillar is fixedly connected to the filling fixed plate, the lower end of the pillar is fixedly connected to the magnet rack, and a plurality of magnetic adsorption magnets are arranged on the side of the magnet rack; the washing liquid needle and the bottom The object needle is located above the rotating disk, the washing liquid needle and the substrate needle are at a certain angle to the vertical direction, the liquid outlet is aligned with each magnet adsorption position, and the washing liquid and substrate pumped out at a certain pressure and speed are used to disperse the concentrated magnetic beads, thereby improving the magnetic bead cleaning effect; the substrate needle is used to add the luminescent reaction substrate, and the substrate needle is directly set in the washing system, so that there is no need to set an independent supporting structure and mounting structure for the substrate needle, and the luminescent substrate can be added after the washing is completed, which simplifies the structure and improves the substrate effect. At the same time, the transfer process of the reaction cup holder from the washing system to the substrate filling system in the traditional equipment is omitted, thereby improving the efficiency;

The waste liquid suction component includes a linear guide rail, a support vertical plate, a guide rail adapter frame, a suction screw motor, a reinforcing rib, a suction waste liquid bracket and a suction waste liquid needle unit. The linear guide rail is fixedly connected to the side of the support vertical plate, the side of the guide rail adapter frame is slidably connected to the linear guide rail through a slider, the support vertical plate is fixedly connected to the upper end of the base plate through the reinforcing rib, the suction screw motor is fixedly connected to the lower end of the base plate, the screw of the suction screw motor passes through the base plate, the screw of the suction screw motor is threadedly connected to the lower part of the guide rail adapter frame, the upper end of the guide rail adapter frame is fixedly connected to the suction waste liquid bracket, the upper end of the suction waste liquid bracket is fixedly connected with a plurality of guide sleeves, and the suction waste liquid needle unit is plugged into the guide sleeves. The rotation of the suction screw motor drives the suction waste liquid bracket to rise and fall, and the linear guide rail plays a guiding and limiting role, making the suction waste liquid bracket rise and fall more smoothly, the suction waste liquid bracket has a uniform up and down amplitude, high controllable accuracy, and the suction waste liquid needle unit has better controllability.

The beneficial effects of the present invention are as follows: the system error generated by using multiple needles to add washing liquid, multiple needles to absorb waste liquid and multiple eccentric rotations for mixing is certain, and the system error can be controlled after debugging to ensure that the amount of washing liquid added to each reaction cup and the residual amount of waste liquid and the mixing effect are consistent, and the washing effect can be guaranteed after repeated washing. The eccentric rotating cup holder that needs to be washed is arranged on a rotating disk, and the rotating disk drives the reaction cup to move under the washing liquid needle and the waste liquid suction needle unit, and the washing liquid needle and the waste liquid suction needle unit point to the eccentric rotation of the reaction cup, respectively. After the different reaction cups in the rotating cup holder are in place, the mixing plate rises under the drive of the mixing screw motor, and the mixing plate lifts up the eccentric rotating cup holder with the reaction cup at the corresponding position, and the washing liquid needle fills the washing liquid into the reaction cup directly below it, and the mixing rotating motor starts to drive each mixing position to perform eccentric rotation and mixing, and the suction screw motor drives the suction waste liquid needle unit to descend, so that the suction waste liquid needle unit sinks into the reaction cup directly below it to absorb the waste liquid, and the washing liquid filling, mixing and waste liquid absorption are carried out simultaneously, which speeds up the washing progress;

The analyzer magnetic bead cleaning device described in the present invention uses multiple washing liquid needles and multiple waste liquid suction needle units to clean each reaction cup, thereby realizing rapid and accurate washing of the reaction cup; a mixing disk is used to enhance the cleaning effect; a rotating disk structure realizes rapid assembly line circulation of the reaction cup, and the control process is modeled to improve efficiency; at the same time, the filling of the luminescent substrate is combined with the washing system, and there is no need to set up an independent supporting structure and mounting structure for the substrate needle. The luminescent substrate can be filled after the washing is completed, thereby eliminating the transfer process of the reaction cup from the washing system to the substrate filling system, simplifying the overall structure and improving efficiency.

On the basis of the above technical solution, the present invention can also be improved as follows:

Furthermore, a reaction cup detection sensor unit is also provided on the base assembly, and the reaction cup detection sensor unit includes a reaction cup detection sensor bracket and a reaction cup detection sensor. The lower end of the reaction cup detection sensor bracket is fixedly connected to the upper end of the base plate, and the upper end of the reaction cup detection sensor bracket is fixedly connected to the reaction cup detection sensor. The reaction cup detection sensor corresponds to the zero point position of the eccentric rotating cup holder.

The beneficial effect of adopting the above further solution is that the reaction cup detection sensor can be used to determine whether there is a reaction cup on the eccentric rotating cup holder, thereby controlling the liquid circuit device and realizing the intelligent liquid filling function when using various reaction cups.

Furthermore, a support column is provided on the base assembly, and the upper end of the support column is fixedly connected to the lower end of the base plate.

The beneficial effect of adopting the above further solution is that the provision of the support column can increase the stability of the body assembly.

Furthermore, a plurality of tension wheels are installed at the lower end of the mixing disk, and the tension wheels, the driving pulley and the mixing position are connected by a synchronous belt.

The beneficial effect of adopting the above further solution is that the setting of the tensioner can adjust the tightness of the synchronous belt, ensuring that the synchronous belt maintains the most suitable tightness, thereby improving the working efficiency of the mixing disk and extending the service life of the synchronous belt.

Furthermore, the structure of the mixing position includes, from top to bottom, a mixing rotating shaft, a rotating bearing, a spacer and a synchronous wheel. The top of the mixing rotating shaft is provided with a convex shaft that matches the hole at the lower end of the eccentric rotating cup holder. The lower end of the mixing rotating shaft passes through the mixing disk. The lower end of the mixing rotating shaft is fixedly connected to the synchronous wheel. The rotating bearing is provided between the mixing rotating shaft and the mixing disk. The mixing rotating shaft is inserted into the rotating bearing. A spacer is provided between the synchronous wheel and the rotating bearing. The rotating bearing is a flange bearing. The inner surfaces of the two flanges are fitted with the mixing disk. The synchronous wheel and the mixing position are fixed to the mixing disk as a whole by a top screw. The synchronous wheel and the driving pulley are connected by a synchronous belt.

The beneficial effect of adopting the above further scheme is that the convex shaft at the upper end of the mixing rotation shaft can be slidably matched with the eccentric rotating cup holder, the eccentric rotating cup holder is used to place the reaction cup, and the mixing rotation motor drives the active pulley to rotate, and the active pulley rotates the synchronous wheel through the synchronous belt, so that the entire mixing position drives the eccentric rotating cup holder to rotate, thereby realizing the eccentric mixing function of the reaction cup. The setting of the spacer can avoid friction between the synchronous wheel and the mixing disk when the mixing position rotates, thereby making the eccentric mixing process smoother.

Furthermore, the eccentric rotating cup holder includes a holder body, a guide rod, a guide pin and a pulling iron sheet are provided at the lower end of the holder body, a clamping ring is provided in the middle of the guide rod, and the lower end of the guide pin is conical; a guide sleeve hole, a pin hole and a magnet hole are provided on the rotating disk, the lower end of the guide rod is slidably matched with the guide sleeve hole, the guide pin is slidably matched with the pin hole, and a pulling magnet is installed in the magnet hole.

The beneficial effect of adopting the above further scheme is that the mixing position rises under the drive of the mixing screw motor, the top of the mixing position is inserted into the two holes at the lower end of the eccentric rotating cup holder, the eccentric rotating cup holder is lifted up, the pulling magnet is disconnected from the pulling iron sheet, the guide pin is separated from the pin hole on the rotating disk, and after the washing liquid is injected into the reaction cup of the eccentric rotating cup holder, the multiple mixing positions are driven by the mixing rotating motor to rotate synchronously, driving the eccentric rotating cup holder to rotate eccentrically, the mixing rotating motor rotates forward at a certain speed, and then reverses at a certain speed, after repeating many times, returns to the original rotation position, the mixing screw motor drives the mixing position to descend, the top of the mixing position is separated from the eccentric rotating cup holder, the pulling magnet is attracted to the pulling iron sheet, the guide pin is inserted into the pin hole of the rotating disk, the position of the eccentric rotating cup holder is locked, the eccentric mixing function of the reaction cup is completed, and one cleaning is completed. The pulling magnet attracts the pulling iron sheet to lock the position of the eccentric rotating cup holder to prevent the eccentric rotating cup holder from accidentally moving when the rotating disk rotates, causing malfunctions; the lower end of the guide pin is tapered for easier insertion and removal

Furthermore, the magnetic attraction magnets on the side of the magnet frame are arranged in groups of three, and a mixing space is provided between each group of the magnetic attraction magnets.

The beneficial effect of adopting the above further solution is that the mixed yielding arrangement can yield when the eccentric rotating cup holder rotates, thereby making the eccentric rotating cup holder rotate more smoothly.

Furthermore, the waste liquid suction needle unit includes a suction needle, a compression spring and a spring nut. The suction needle is inserted into the guide sleeve, the lower end of the compression spring is installed on the upper end of the guide sleeve, the upper end of the compression spring is installed with the spring nut, the upper end of the suction needle is provided with an interface for connecting to a negative pressure source, and the lower end of the suction needle is provided with a straight groove.

The beneficial effects of adopting the above further scheme are: the setting of the compression spring and the spring nut enables the suction needle to completely contact the bottom of the cup when it descends to extract the waste liquid in the reaction cup; the groove prevents the suction needle from being blocked when it contacts the bottom of the cup, ensuring that the waste liquid is completely emptied; the upper part of the suction needle can be connected to a negative pressure source such as a vacuum pump or a peristaltic pump, thereby realizing automatic suction of the waste liquid in the reaction cup by the suction needle.

Furthermore, a sensor bracket is fixedly connected to the upper end of the waste liquid suction bracket, and a fault sensor is fixedly connected to the upper end of the sensor bracket.

The beneficial effect of adopting the above further scheme is that when the analyzer magnetic bead cleaning device fails, the induction effect of the fault sensor causes the aspiration needle to be lifted up, triggering an alarm to protect the safety of the aspiration needle and even the entire analyzer magnetic bead cleaning device.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic structural diagram of a magnetic bead cleaning device for an analyzer in an embodiment;

FIG2 is a schematic structural diagram of a base assembly in an embodiment;

FIG3 is a schematic diagram of the structure of a mixing assembly in an embodiment;

FIG4 is a schematic diagram of the structure of a mixing station in an embodiment;

FIG5 is a schematic structural diagram of a rotating assembly in an embodiment;

FIG6 is a schematic structural diagram of an eccentric rotating cup holder in an embodiment;

FIG7 is a schematic diagram of the structure of a rotating disk in an embodiment;

FIG8 is a partial enlarged schematic diagram of the rotating disk in the embodiment;

FIG9 is a schematic structural diagram of a lotion filling assembly in an embodiment;

FIG10 is a schematic structural diagram of a waste liquid suction assembly in an embodiment;

FIG11 is a partial enlarged view of the lower port of the aspiration needle in the embodiment;

In the figure, 1 is a base assembly, 2 is a mixing assembly, 3 is a rotating assembly, 4 is a washing liquid filling assembly, 5 is a waste liquid suction assembly, and 6 is a synchronous belt;

11 bottom plate, 12 bearing seat, 13 rotating base shaft, 14 rotating motor, 15 mixing lifting guide shaft, 16 driving pulley, 17 driven pulley, 18 reaction cup detection sensor bracket, 19 reaction cup detection sensor, 20 support column;

21 mixing plate, 22 mixing position, 23 mixing screw motor, 24 mixing rotary motor, 25 motor bracket, 26 driving pulley, 27 tensioning pulley, 28 screw nut, 29 mixing lifting linear bearing, 210 lifting sensor contact sheet;

221 mixing rotating shaft, 222 rotating bearing, 223 spacer ring, 224 synchronous wheel;

31 rotating plate, 32 eccentric rotating cup holder;

311 guide sleeve hole, 312 pin hole, 313 magnet hole, 314 pulling magnet; 321 support body, 322 guide rod, 323 guide pin, 324 pulling iron sheet, 325 clamping ring;

41 filling fixing plate, 42 washing solution needle, 43 washing solution filling support, 44 substrate needle, 45 injection needle support, 46 support, 47 magnet support, 48 magnetic attraction magnet, 49 mixing and giving way;

51 linear guide rail, 52 supporting plate, 53 guide rail adapter frame, 54 suction screw motor, 55 reinforcing rib, 56 suction waste liquid bracket; 571 suction needle, 572 compression spring, 573 spring nut; 58 guide sleeve, 59 sensor bracket, 510 fault sensor.

DETAILED DESCRIPTION

In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

As shown in FIG1 , a magnetic bead cleaning device for an analyzer includes, from bottom to top, a base component 1, a mixing component 2, a rotating component 3, a washing liquid filling component 4, and a waste liquid suction component 5;

As shown in Figure 2, the base assembly 1 includes a base plate 11, a bearing seat 12, a rotating base shaft 13, a rotating motor 14 and a mixing and lifting guide shaft 15. The upper end of the bearing seat 12 is fixedly connected to the base plate 11, the lower end of the rotating base shaft 13 passes through the base plate 11, the lower end of the rotating base shaft 13 is connected to the bearing seat 12 through a bearing, the rotating motor is fixedly connected to the lower end of the base plate 11, the shaft of the rotating motor 14 passes through the base plate 11, the shaft of the rotating motor 14 is provided with a driving pulley 16, the rotating base shaft 13 is provided with a driven pulley 17, the driving wheel and the driven pulley 17 are connected through a synchronous belt 6, and the lower end of the mixing and lifting guide shaft 15 is fixedly connected to the base plate 11 through a guide shaft support.

As shown in Figure 3, the mixing component 2 includes a mixing disk 21, a mixing position 22, a mixing screw motor 23 and a mixing rotary motor 24. The mixing disk 21 is provided with a plurality of mixing positions 22, and the mixing positions 22 are rotatably connected to the mixing disk 21 through bearings. The lower end of the mixing disk 21 is fixedly connected to a motor bracket 25, and the lower end of the motor bracket 25 is fixedly connected to the mixing rotary motor 24. The shaft of the mixing motor passes through the upper end of the motor bracket 25, and the upper end of the shaft of the mixing motor is provided with a driving pulley 26, and the driving pulley 26 is connected to the mixing position 22 through a synchronous belt 6. The lower end of the mixing disk 21 is installed with a plurality of tensioners 27, and the tensioner 27, the driving pulley 26 and the mixing position 22 are connected through a synchronous belt 6. The lower end of the mixing disk 21 is provided with a screw nut 28, the mixing screw motor 23 is fixedly connected to the lower end of the base plate 11, the shaft of the mixing screw motor 23 passes through the base plate 11, and the screw of the mixing screw motor 23 is connected to the screw nut 28 by threads; the lower end of the mixing disk 21 is fixedly connected to a mixing lifting linear bearing 29, the upper end of the mixing lifting guide shaft 15 is slidably connected to the mixing lifting linear bearing 29, and the lower end of the mixing disk 21 is fixedly connected to a lifting sensor contact piece 210;

As shown in FIG5 , the rotating assembly 3 comprises a rotating disk 31 and an eccentric rotating cup holder 32. The rotating base shaft 13 passes through the mixing assembly 2. The rotating base shaft 13 is fixedly connected to the rotating disk 31. A plurality of eccentric rotating cup holders 32 are provided at the upper end of the rotating disk 31. The eccentric rotating cup holders 32 are slidably matched with the rotating disk 31. The lower end of the eccentric rotating cup holder 32 is provided with a hole position matched with the upper end of the mixing position 22. The hole position at the lower end of the eccentric rotating cup holder 32 is slidably matched with the upper end of the mixing position 22 on the mixing disk 21. The rotating disk 31 is mounted on the rotating base shaft 13 and rotates under the action of the rotating motor 14. Only one corresponding hole position is rotated each time. The eccentric distance of the eccentric rotating cup holder 32 can be adjusted according to actual use.

As shown in Figure 9, the washing liquid filling component 4 includes a filling fixed plate 41, a washing liquid needle 42, a washing liquid filling bracket 43, a substrate needle 44, an injection needle rack 45, a pillar 46 and a magnet rack 47. The lower end of the washing liquid filling bracket 43 is fixedly connected to the bottom plate 11, and the upper end of the washing liquid filling bracket 43 is fixedly connected to the filling fixed plate 41. The upper end of the filling fixed plate 41 is fixedly connected with a plurality of injection needle racks 45. The substrate needle 44 is plugged into the injection needle rack 45, and a plurality of the washing liquid needles 42 are plugged into the injection needle rack 45. The injection needle rack 45 has a certain angle, so that the outlet water flow of the filling needle is just sprayed onto the adsorbed magnetic bead group to disperse it. The upper end of the support 46 is fixedly connected to the filling fixing plate 41, and the lower end of the support 46 is fixedly connected to the magnet frame 47. A plurality of magnetic attraction magnets 48 are provided on the side of the magnet frame 47. The magnetic attraction magnets 48 on the side of the magnet frame 47 are grouped in three, and a mixing space 49 is provided between each group of the magnetic attraction magnets 48, so as to make way for the eccentric rotating cup holder 32 when rotating. The front side of the magnetic attraction magnet 48 is the inner side of the reaction cup, and the magnetic beads in the reaction cup can be adsorbed. In this embodiment, each magnet adsorbs the reaction cup for 20 seconds.

As shown in Figure 10, the suction waste liquid assembly 5 includes a linear guide rail 51, a support vertical plate 52, a guide rail adapter frame 53, a suction screw motor 54, a reinforcing rib 55, a suction waste liquid bracket 56 and a suction waste liquid needle unit. The linear guide rail 51 is fixedly connected to the side of the support vertical plate 52, and the side of the guide rail adapter frame 53 is slidably connected to the linear guide rail 51 through a slider. The support vertical plate 52 is fixedly connected to the upper end of the base plate 11 through the reinforcing rib 55. The suction screw motor 54 is fixedly connected to the lower end of the base plate 11. The screw of the suction screw motor 54 passes through the base plate 11, and the screw of the suction screw motor 54 is threadedly connected to the lower part of the guide rail adapter frame 53. The upper end of the guide rail adapter frame 53 is fixedly connected to the suction waste liquid bracket 56. The upper end of the suction waste liquid bracket 56 is fixedly connected with a plurality of guide sleeves 58, and the suction waste liquid needle unit is inserted into the guide sleeves 58.

The base component 1 is also provided with a reaction cup detection sensor unit, which includes a reaction cup detection sensor bracket 18 and a reaction cup detection sensor 19. The lower end of the reaction cup detection sensor bracket 18 is fixedly connected to the upper end of the bottom plate 11, and the upper end of the reaction cup detection sensor bracket 18 is fixedly connected to the reaction cup detection sensor 19. The reaction cup detection sensor 19 corresponds to the zero point position of the eccentric rotating cup holder 32. The reaction cup detection sensor 19 is used to determine whether there is a reaction cup, control the liquid circuit device according to the situation, and realize the intelligent liquid filling function that adapts to the situation of various reaction cups. The base component 1 is also provided with a plurality of support columns 20, and the upper end of the support column 20 is fixedly connected to the lower end of the bottom plate 11. The setting of the support column 20 can increase the stability of the base component 1.

As shown in Figure 4, the structure of the mixing position 22 includes a mixing rotating shaft 221, a rotating bearing 222, a spacer 223 and a synchronous wheel 224 from top to bottom. The top of the mixing rotating shaft 221 is provided with a convex shaft that matches the hole at the lower end of the eccentric rotating cup holder 32. The lower end of the mixing rotating shaft 221 passes through the mixing disk 21. The lower end of the mixing rotating shaft 221 is fixedly connected to the synchronous wheel 224. The rotating bearing 222 is provided between the mixing rotating shaft 221 and the mixing disk 21. The mixing rotating shaft 221 is inserted into the rotating bearing 222. A spacer 223 is provided between the synchronous wheel 224 and the rotating bearing 222. The rotating bearing 222 is a flange bearing, and the inner surfaces of the two flanges are fitted with the mixing disk 21. The synchronous wheel 224 and the mixing position 22 are fixed to the mixing disk 21 as a whole by a top screw, and the synchronous wheel 224 is connected to the driving pulley 26 by a synchronous belt 6.

As shown in Figs. 6-8, the eccentric rotating cup holder 32 comprises a holder body 321, the lower end of which is provided with a guide plug rod 322, a guide pin 323 and a pulling iron sheet 324, the middle of the guide plug rod 322 is provided with a clamping ring 325, and the lower end of the guide pin 323 is conical; the rotating disk 31 is provided with a guide sleeve hole 311, a pin hole 312 and a magnet hole 313, the lower end of the guide plug rod 322 is slidably matched with the guide sleeve hole 311, the guide pin 323 is slidably matched with the pin hole 312, and a pulling magnet 314 is installed in the magnet hole 313. The three characteristic holes of the guide sleeve hole 311, the pin hole 312 and the magnet hole 313 are a group. In this embodiment, there are 22 groups on the rotating disk 31, and they can be increased or decreased according to the actual situation in actual use. Under normal circumstances, after the reaction cup is mixed, the guide pin 323 is inserted into the pin hole 312 opened on the rotating disk 31, and the guide plug rod 322 is inserted into the guide plug hole 311. At this time, the pulling iron sheet 324 is located directly above the pulling magnet 314, and the pulling magnet 314 attracts the pulling iron sheet 324 to lock the position of the eccentric rotating cup holder 32 to prevent the eccentric rotating cup holder 32 from accidentally moving when the rotating disk 31 rotates, causing a malfunction. The lower end of the guide pin 323 is tapered for easy insertion and removal.

As shown in Figures 10-11, the waste liquid suction needle unit includes a suction needle 571, a compression spring 572 and a spring nut 573. The suction needle 571 is plugged into the guide sleeve 58, the lower end of the compression spring 572 is installed on the upper end of the guide sleeve 58, and the spring nut 573 is installed on the upper end of the compression spring 572. The upper end of the suction needle 571 is provided with an interface for connecting a negative pressure source, and the lower end of the suction needle 571 is provided with a groove with a letter "1". The suction needle 571 is located at a position 1mm away from the center of the eccentric rotating cup holder 32. When extracting waste liquid, it can be away from the magnetic beads, so that the effect of the liquid extraction action on the magnetic beads is minimized. The compression spring 572 allows the suction needle 571 to fully contact the bottom of the cup when it descends to extract waste liquid, and the groove prevents it from being blocked when contacting the bottom of the cup, ensuring that the waste liquid is completely emptied. In this embodiment, a total of 5 groups of suction needles 571 are provided, which can be adjusted as needed during actual use. In this embodiment, the suction needle 571, the washing needle 42 and the substrate needle 44 are distributed at equal angles on their respective brackets, with one of four eccentric rotating cup holders 32 arranged, with the suction needle 571 in front and the washing needle 42 next to the eccentric rotating cup holder 32 arranged adjacently and staggered.

The upper end of the waste liquid suction bracket 56 is fixedly connected to a sensor bracket 59, and the upper end of the sensor bracket 59 is fixedly connected to a fault sensor 510. The fault sensor 510 enables the suction needle 571 to be lifted up and trigger an alarm when a fault occurs in the magnetic bead cleaning device of the analyzer, so as to protect the safety of the suction needle 571 and even the entire magnetic bead cleaning device of the analyzer.

Working principle: the reaction cup detection sensor 19 detects that there is a reaction cup on the eccentric rotating cup holder 32. At this time, the guide pin 323 of the eccentric rotating cup holder 32 is inserted into the pin hole 312 opened on the rotating disk 31, and the pulling magnet 314 attracts the pulling iron sheet 324. The magnetic attraction magnet 48 on the magnet frame 47 attracts the magnetic beads in the reaction cup for 20 seconds. The rotating disk 31 moves to the next position under the drive of the rotating motor 14. The magnetic attraction magnet 48 on the magnet frame 47 attracts the magnetic beads in the reaction cup for 20 seconds. The rotating disk 31 moves to the next position under the drive of the rotating motor 14. The magnetic attraction magnet 48 on the magnet frame 47 attracts the magnetic beads in the reaction cup for 20 seconds, and the total adsorption is 60 seconds. Under the drive of the suction screw motor 54, the suction needle 571 descends to evacuate the liquid in the reaction cup, and then the suction needle 571 rises and returns to the origin. The rotating disk 31 moves to the next position under the drive of the rotating motor 14. The mixing position The mixing screw motor 23 drives the mixing rotating shaft 22 to rise, and the top of the mixing rotating shaft 221 is inserted into the two holes at the lower end of the eccentric rotating cup holder 32 to lift the eccentric rotating cup holder 32, and the pulling magnet 314 is disconnected from the pulling iron sheet 324, and the guide pin 323 is separated from the pin hole 312 on the rotating disk 31, and the lotion filling assembly 4 injects lotion, and the multiple mixing rotating shafts 221 rotate synchronously under the drive of the mixing rotating motor 24, driving the eccentric rotating cup holder 32 to rotate eccentrically. The mixing rotating motor 24 rotates forward at a certain speed, and then reverses at a certain speed. After repeating for many times, it returns to the original rotation position. The mixing screw motor 23 drives the mixing position 22 to descend, and the mixing rotating shaft 221 eccentrically rotates the cup holder 32. The pulling magnet 314 and the pulling iron sheet 324 are attracted. The guide pin 323 is inserted into the pin hole 312 opened on the rotating disk 31 to lock the position of the eccentric rotating cup holder 32, and the eccentric mixing function of the reaction cup is completed. At this point, one cleaning is completed, and the above steps are repeated. A total of 4 times of washing liquid are added, 5 times of waste liquid are extracted, and finally after the substrate is added and mixed, the rotating disk 31 moves to the initial position under the drive of the rotating motor 14, and the cleaning is completed. The reaction cup is taken away for the next operation. It should be pointed out that each time the rotating disk 31 moves to the next position under the drive of the rotating motor 14, the cleaned reaction cup can be taken out at the initial position and a new reaction cup that needs to be cleaned can be placed. This cycle is fully automated and batch operations can be performed on multiple reaction cups, which is beneficial to saving operation time and improving work efficiency.

The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims (9)

1. A magnetic bead cleaning device of an analyzer is characterized in that,

The analyzer magnetic bead cleaning device comprises a base body assembly (1), a mixing assembly (2), a rotating assembly (3), a washing liquid filling assembly (4) and a waste liquid sucking assembly (5) from bottom to top in sequence;

The base assembly (1) comprises a bottom plate (11), a bearing seat (12), a rotary base shaft (13), a rotary motor (14) and a mixing lifting guide shaft (15), wherein the upper end of the bearing seat (12) is fixedly connected with the bottom plate (11), the lower end of the rotary base shaft (13) penetrates through the bottom plate (11), the lower end of the rotary base shaft (13) is connected with the bearing seat (12) through a bearing, the rotary motor (14) is fixedly connected with the lower end of the bottom plate (11), a shaft rod of the rotary motor (14) penetrates through the bottom plate (11), a driving belt wheel (16) is arranged on the shaft rod of the rotary motor (14), a driven belt wheel (17) is arranged on the rotary base shaft (13), the driving belt wheel (16) is connected with the driven belt wheel (17) through a synchronous belt (6), and the lower end of the mixing lifting guide shaft (15) is fixedly connected with the bottom plate (11) through a guide shaft support.

The mixing assembly (2) comprises a mixing disc (21), mixing positions (22), a mixing screw motor (23) and a mixing rotating motor (24), a plurality of mixing positions (22) are arranged on the mixing disc (21), the mixing positions (22) are rotationally connected with the mixing disc (21) through bearings, a motor support (25) is fixedly connected to the lower end of the mixing disc (21), the lower end of the motor support (25) is fixedly connected with the mixing rotating motor (24), a shaft rod of the mixing rotating motor (24) penetrates through the upper end of the motor support (25), a driving belt pulley (26) is arranged at the upper end of the shaft rod of the mixing rotating motor (24), and the driving belt pulley (26) is connected with the mixing positions (22) through a synchronous belt (6); the lower end of the mixing disc (21) is provided with a screw nut (28), the mixing screw motor (23) is fixedly connected to the lower end of the bottom plate (11), a shaft rod of the mixing screw motor (23) penetrates through the bottom plate (11), and a screw rod of the mixing screw motor (23) is connected with the screw nut (28) through threads; the lower end of the mixing disc (21) is fixedly connected with a mixing lifting linear bearing (29), the upper end of the mixing lifting guide shaft (15) is in sliding connection with the mixing lifting linear bearing (29), and the lower end of the mixing disc (21) is fixedly connected with a lifting sensing contact piece (210);

The rotary assembly (3) comprises a rotary disc (31) and eccentric rotary cup holders (32), the rotary base shaft (13) penetrates through the mixing assembly (2), the rotary base shaft (13) is fixedly connected with the rotary disc (31), a plurality of eccentric rotary cup holders (32) are arranged at the upper end of the rotary disc (31), the eccentric rotary cup holders (32) are in sliding fit with the rotary disc (31), and hole sites matched with the upper ends of the mixing positions (22) are arranged at the lower ends of the eccentric rotary cup holders (32);

The washing liquid filling assembly (4) comprises a filling fixing plate (41), a washing liquid needle (42), a washing liquid filling support (43), substrate needles (44), a liquid filling needle frame (45), a support column (46) and a magnet frame (47), wherein the lower end of the washing liquid filling support (43) is fixedly connected with the bottom plate (11), the upper end of the washing liquid filling support (43) is fixedly connected with the filling fixing plate (41), the upper end of the filling fixing plate (41) is fixedly connected with a plurality of liquid filling needle frames (45), the substrate needles (44) are inserted on the liquid filling needle frames (45), the washing liquid needles (42) are inserted on the liquid filling needle frames (45), the upper end of the support column (46) is fixedly connected with the filling fixing plate (41), the lower end of the support column (46) is fixedly connected with the magnet frame (47), and a plurality of magnetic adsorption magnets (48) are arranged on the side surfaces of the magnet frame (47).

The waste liquid sucking component (5) comprises a linear guide rail (51), a supporting vertical plate (52), a guide rail switching frame (53), a suction lead screw motor (54), a reinforcing rib (55), a suction lead screw motor (54) and a waste liquid sucking needle unit, wherein the linear guide rail (51) is fixedly connected to the side surface of the supporting vertical plate (52), the side surface of the guide rail switching frame (53) is in sliding connection with the linear guide rail (51) through a sliding block, the supporting vertical plate (52) is fixedly connected with the upper end of the bottom plate (11) through the reinforcing rib (55), the suction lead screw motor (54) is fixedly connected with the lower end of the bottom plate (11), the lead screw of the suction lead screw motor (54) penetrates through the bottom plate (11), the lead screw of the suction lead screw motor (54) is in threaded connection with the lower portion of the guide rail switching frame (53), the upper end of the guide rail switching frame (53) is fixedly connected with the suction lead screw bracket (56), and the upper end of the suction lead screw bracket (56) is fixedly connected with a plurality of guide sleeves (58), and the suction lead screw motor (54) is fixedly connected with the waste liquid sucking needle unit on the guide sleeve (58).

2. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

Still be equipped with reaction cup on base member subassembly (1) and detect sensing unit, reaction cup detects sensing unit and detects sensing support (18) and reaction cup and detect sensor (19) including reaction cup, reaction cup detects sensing support (18) lower extreme fixed connection in the upper end of bottom plate (11), reaction cup detects sensing support (18) upper end fixed connection reaction cup detects sensor (19), reaction cup detect sensor (19) with the zero point position of eccentric rotatory saucer (32) body is corresponding.

3. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

The base assembly (1) is further provided with a support column (20), and the upper end of the support column (20) is fixedly connected to the lower end of the bottom plate (11).

4. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

A plurality of tensioning wheels (27) are arranged at the lower end of the mixing disc (21), and the tensioning wheels (27), the driving belt wheels (26) and the mixing positions (22) are connected through a synchronous belt (6).

5. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

The structure of mixing position (22) from last to including mixing rotation axis (221), swivel bearing (222), spacer ring (223) and synchronizing wheel (224), the top of mixing rotation axis (221) be equipped with eccentric rotatory saucer (32) lower extreme hole phase complex protruding axle, mixing rotation axis (221) lower extreme passes mixing dish (21), mixing rotation axis (221) lower extreme with synchronizing wheel (224) fixed connection, mixing rotation axis (221) with be equipped with between mixing dish (21) swivel bearing (222), mixing rotation axis (221) are inserted swivel bearing (222), synchronizing wheel (224) with be equipped with spacer ring (223) between swivel bearing (222), swivel bearing (222) are flange bearing, two flange inner faces with mixing dish (21) laminating, will through the jackscrew synchronizing wheel (224) with mixing position (22) are whole to be fixed on mixing dish (21), synchronizing wheel (224) and synchronizing belt (26) pass through coupling 6.

6. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

The eccentric rotary cup stand (32) comprises a stand body (321), a guide inserted rod (322), a guide pin (323) and a pulling iron sheet (324) are arranged at the lower end of the stand body (321), a clamping ring (325) is arranged in the middle of the guide inserted rod (322), and the lower end of the guide pin (323) is conical; the rotary disk (31) is provided with a guide sleeve hole (311), a pin hole (312) and a magnet hole (313), the lower end of the guide sleeve rod (322) is in sliding fit with the guide sleeve hole (311), the guide pin (323) is in sliding fit with the pin hole (312), and a pulling magnet (314) is arranged in the magnet hole (313).

7. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

The magnetic attraction magnets (48) on the side face of the magnet frame (47) are arranged in groups of three, and a mixed abdication (49) is arranged between each group of the magnetic attraction magnets (48).

8. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

The waste liquid pumping needle unit comprises a pumping needle (571), a compression spring (572) and a spring pressing nut (573), the pumping needle (571) is inserted on the guide sleeve (58), the lower end of the compression spring (572) is installed at the upper end of the guide sleeve (58), the spring pressing nut (573) is installed at the upper end of the compression spring (572), an interface for connecting a negative pressure source is arranged at the upper end of the pumping needle (571), and a groove in a shape is formed in the lower port of the pumping needle (571).

9. The apparatus for washing magnetic beads of an analyzer according to claim 1, wherein,

The upper end of the waste liquid sucking bracket (56) is fixedly connected with a sensor bracket (59), and the upper end of the sensor bracket (59) is fixedly connected with a fault sensor (510).