CN111534423A - A kind of group nucleic acid testing mixing device and method - Google Patents

Abstract

The invention discloses a group nucleic acid testing mixing device and method, comprising a sampling bottle positioning and placing plate, a mixing bottle placing plate, a disinfection device, an automatic suction device and a rotary console for positioning and rotating the automatic suction device, and positioning and placing the sampling bottle The plate, the mixing bottle placing plate and the sterilizing device are fixed on one side of the rotating console, and a plurality of sampling bottle placing slots are arranged on the sampling bottle positioning plate. The rapid positioning of the automatic suction device absorbs the samples to be detected stored in the sampling bottle to be detected, so as to obtain a mixture of multiple samples to be detected. By detecting the mixed sample liquid, the detection efficiency is improved. The device has a simple structure, high safety, and avoids work The misplacement and mishandling of samples caused by personnel errors improves the safety of testing samples, and at the same time avoids the number of manual contacts during the mixing process and improves the safety of use.

Description

A kind of group nucleic acid testing mixing device and method

technical field

The invention belongs to a medical detection device, and in particular relates to a group nucleic acid test mixing device and method.

Background technique

COVID-19 has swept the world, and WHO recommends using an evidence-based model to respond to the outbreak, emphasizing "testing" to achieve virus detection and confirmation through testing. However, due to the strong infectivity of the virus, in theory, simultaneous testing is required, that is, the confirmation of a large number of samples is completed within a short period of time, that is, to improve the efficiency of virus sample confirmation and prevent the rapid spread of the virus. In order to improve the efficiency of virus detection and confirmation, currently, single-body kits are mainly used for sample detection, which is inefficient and cannot effectively complete the detection of a large number of samples in a short time.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a population nucleic acid testing mixing device and method to overcome the deficiencies of the prior art.

To achieve the above object, the present invention adopts the following technical solutions:

A group nucleic acid testing mixing device, comprising a sampling bottle positioning and placing plate, a mixing bottle placing plate, a sterilizing device, an automatic suction device and a rotary console for positioning and rotating the automatic suction device, a sampling bottle positioning and placing plate, a mixing bottle placing plate and The disinfection device is fixed on one side of the rotating console, the sampling bottle positioning tray is provided with a plurality of sampling bottle placement slots, the sampling bottles to be tested are placed in the sampling bottle placement slots, and the samples to be tested are stored in the sampling bottles to be tested; the rotating console There is a mechanical arm that can drive the automatic suction device to rotate and move, and the sampling bottle positioning and placing plate, the mixing bottle placing plate and the sterilizing device are fixed within the moving range of the automatic suction device to pick up and place the liquid.

Further, the sampling bottle placement slots are arrayed on the sampling bottle positioning and placement tray, and each sampling bottle placement slot has a relative positioning position number with the rotating console.

Further, a positioning and placing sleeve is provided in the sampling bottle placing groove.

Further, the positioning and placing sleeve is a rubber sleeve, and the positioning and placing sleeve is adhesively connected with the groove wall of the sampling bottle placing groove.

Further, the disinfection device includes a clean water pool, a sewage pool and a disinfection pool, and the automatic suction device can be driven into the clean water pool, the sewage pool and the disinfection pool by a mechanical arm.

Further, the automatic suction device includes a needle tube, a needle tube clamp and an electric push rod, the needle tube clamp and the electric push rod are fixed on the end of the mechanical arm through the fixing plate, the end of the piston rod of the needle tube is fixed on the needle tube clamp, and the outer wall of the needle tube of the needle tube is fixed on the needle tube clamp. A clamp is fixedly clamped, and the clamp is fixed to the end of the electric push rod through a clamp rod.

Further, one side of the needle tube clamp is provided with an opening, and one side of the needle tube clamp is provided with an opening and a bottom part is provided with a clamping groove, the end of the piston rod is clamped in the clamping groove, and the end of the clamping groove is locked by a screw rod.

Further, the robotic arm on the rotating console adopts coordinate positioning or image recognition positioning.

A population nucleic acid testing method, comprising the following steps:

Step 1), put a plurality of sampling bottle numbers to be detected containing the samples to be detected into the sampling bottle placement groove of the sampling bottle positioning plate in turn;

Step 2), using the automatic suction device to extract the sampling liquid from each sampling bottle to be detected in turn, and then put it into the mixing bottle to obtain the mixed sampling liquid. The suction device is sterilized once;

Step 3), then test the mixed sampling liquid in the mixing bottle, if the test result is negative, then end this test, if the test result is positive, then according to the middle-point dyeing method to collect a plurality of samples to be tested this time. The bottle 6 is divided into two half-areas during the process, and steps 1) to 3) are repeated for each half-area to detect until the smallest half-area unit is a sampling bottle to be detected, that is, the detection of multiple detection samples is completed.

Further, PCR is used to perform amplification test on the sampled liquid in the mixing bottle.

Compared with the prior art, the present invention has the following beneficial technical effects:

The present invention is a group nucleic acid testing mixing device, comprising a sampling bottle positioning and placing plate, a mixing bottle placing plate, a disinfection device, an automatic suction device and a rotating console for positioning and rotating the automatic suction device. The sampling bottle is positioned and placed on the plate and the mixing bottle The placing plate and the sterilizing device are fixed on one side of the rotating console, and a plurality of sampling bottle placing slots are arranged on the sampling bottle positioning plate. Quickly locate and absorb the samples to be detected stored in the sampling bottle to be detected, so as to obtain a mixture of multiple samples to be detected. By detecting the mixed sample liquid, the detection efficiency is improved. The device has a simple structure and high safety. The sample is misplaced and taken by mistake, which improves the detection safety of the test sample, and at the same time avoids the number of manual contacts during the mixing process, and improves the safety of use.

Further, the positioning and placing sleeve adopts a rubber sleeve, and the positioning and placing sleeve is adhesively connected with the groove wall of the sampling bottle placing groove, so as to ensure the placement stability of the sampling bottle to be tested.

Further, the needle tube clamp and the electric push rod are used to realize the suction of the needle tube, the structure is simple, the common needle tube can be matched, the application scope is wide, and the emergency use efficiency of the device is improved.

Further, a group nucleic acid testing method, by sequentially placing a plurality of sampling bottle numbers to be tested containing samples to be tested into the sampling bottle placement groove of the sampling bottle positioning tray, and using an automatic suction device to sequentially and sequentially from each to-be-detected sample bottle. Extract the sampling liquid from the detection sampling bottle and put it into the mixing bottle to obtain the mixed sampling liquid, and then test the mixed sampling liquid in the mixing bottle. Multiple sampling bottles to be tested are divided into two half-zones during the process, and then the detection is carried out in sequence until the smallest half-zone unit is a sampling bottle to be tested. Using this method, the detection of multiple samples can be quickly checked, and all samples can be quickly checked. A community with zero infection and a small number of infections has geometrically improved the testing speed of the test samples with a small number of infections and improved the detection efficiency.

Description of drawings

FIG. 1 is a schematic structural diagram of an embodiment of the present invention.

FIG. 2 is a top view of the structure in the embodiment of the present invention.

FIG. 3 is a schematic structural diagram of an automatic suction device in an embodiment of the present invention.

FIG. 4 is a schematic diagram of the structure of the test by dividing the dyeing group in the embodiment of the present invention.

In the figure, 1. Sampling bottle positioning and placing plate; 2. Automatic suction device; 3. Rotating console; 4. Robot arm; 5. Sampling bottle placing slot; 6. Sampling bottle to be tested; 7. Mixing bottle placing plate; 8 , disinfection device; 9, mixing bottle; 10, clear water pool; 11, sewage pool; 12, disinfection pool; 13, camera; 14, light source; 15, needle tube clamp; 16, electric push rod; 17, needle tube; 18, screw ; 19, fixture; 20, fixed plate; 21, clamping rod; 22, piston rod; 23, support rod.

Detailed ways

Below in conjunction with accompanying drawing, the present invention is described in further detail:

As shown in Figures 1 and 2, a group nucleic acid testing mixing device includes a sampling bottle positioning and placing plate 1, a mixing bottle placing plate 7, a sterilizing device 8, an automatic suction device 2 and a rotation for the positioning and rotation of the automatic suction device 2 The console 3, the sampling bottle positioning tray 1, the mixing bottle positioning tray 7 and the disinfection device 8 are fixed on one side of the rotating console 3. The sampling bottle positioning tray 1 is provided with a plurality of sampling bottle placement slots 5, and the sampling bottles to be tested are 6 is placed in the sampling bottle placement slot 5, and the sampling bottle 6 to be tested stores the sample to be tested; the rotating console 3 is provided with a mechanical arm 4 that can drive the automatic suction device 2 to rotate and move, and the sampling bottle is positioned on the placement plate 1 and the mixing bottle. The placing plate 7 and the sterilizing device 8 are within the range of the automatic suction device 2 to move and pick up and place the liquid. The robotic arm 4 can drive the automatic suction device 2 to absorb the liquid in the sampling bottle 6 to be detected on the positioning and placing plate 1 of the sampling bottle, and move the liquid to place. The mixing bottle is placed in the mixing bottle 9 on the tray 7 , and the sterilizing device 8 is used for sterilizing the automatic suction device 2 . The sampling bottle 6 to be tested is provided with a rubber cap, which is convenient for the long needle of the syringe to be inserted and withdrawn, and the puncture hole is automatically closed to prevent the sampling liquid from volatilizing.

The sampling bottle placing slots 5 are arrayed on the sampling bottle positioning and placing plate 1, and each sampling bottle placing slot 5 has a positioning position number. The sampling bottle placing slot 5 is provided with a positioning placing sleeve for positioning and placing the sampling bottle 6 to be detected. To prevent the sampling bottle 6 to be tested from shaking during the liquid extraction process, the positioning and placing sleeve adopts a rubber sleeve, and the positioning and placing sleeve is glued to the wall of the sampling bottle placing groove 5 groove.

The disinfection device 8 includes a clean water tank 10 , a sewage tank 11 and a disinfection tank 12 . The automatic suction device 2 can be driven into the clean water tank 10 , the sewage tank 11 and the disinfection tank 12 by the mechanical arm 4 for disinfection of the automatic suction device 2 . The clean water pool 10, the sewage pool 11 and the disinfection pool 12 can be separately integrated into one, which is convenient for uniform disinfection after detection and improves the safety of the equipment; Integrated into one, after a batch of samples is detected, it is convenient for uniform disinfection of equipment.

As shown in FIG. 3 , the automatic suction device 2 includes a needle tube 17 , a needle tube clamp 15 and an electric push rod 16 . The needle tube clamp 15 and the electric push rod 16 are fixed to the end of the mechanical arm 4 through the fixing plate 20 , and the piston rod 22 of the needle tube 17 The end is fixed on the needle tube clamp 15, the outer wall of the needle tube 17 is fixedly clamped with a clamp 19, the clamp 19 is fixed to the end of the electric push rod 16 through the clamping rod 21, and the electric push rod 16 is used to drive the needle tube 17 through the clamp 19. The needle of the needle tube 17 moves, so that the needle at the end of the needle tube 17 sucks the liquid in the sampling bottle 6 to be detected. During use, the syringe of the needle tube 17 is driven by the mechanical arm 4 to make the needle pierce into the sampling bottle 6 to be detected, and then the electric push rod 16 pushes the needle tube of the needle tube 17 to move while the robot arm 4 is lifted, so that the needle tube 17 is moved. The syringe and the piston rod 22 produce relative movement to achieve liquid suction.

One side of the needle tube clamp 15 is provided with an opening, and one side of the needle tube clamp 15 is provided with a slot on the bottom of the opening.

The robotic arm 4 on the rotating console 3 adopts coordinate positioning or image recognition positioning; using coordinate positioning, that is, taking the rotation center of the rotating console 3 as the coordinate center, the sampling bottle is positioned and placed on the sampling bottle placement slot 5 and mixing bottle on the tray 1. The coordinates of the mixing bottle and the sterilizing device 8 placed on the tray 7 are fixed. Using image recognition and positioning, including camera 13 and light source 14, rubber bottle caps are set on the sampling bottle 6 to be detected, and the color of the rubber bottle cap is different from the color of other positions, which is used for the positioning and recognition of the camera 13, and the visual recognition is carried out according to the image captured by the camera, Position the sample bottle 6 to be detected, move the automatic suction device 2 to the top of the sample bottle 6 to be detected by the robotic arm 4, adjust the robot hand posture according to the captured image, and place the syringe of the automatic suction device 2 on the sample to be detected. Just above the bottle 6, then press down, insert the needle into the sampling bottle 6 to be detected, control the electric push rod of the automatic suction device 2, move the syringe piston of the automatic suction device 2, and absorb the liquid from the sampling bottle 6 to be detected; The volume of each sampling liquid meets the minimum liquid volume requirements for detection, that is, the liquid drawn from a single sampling bottle 6 to be detected each time can represent the sample detected in the sampling bottle 6 to be detected, and realize sample identification; the volume of the mixing bottle 9 is greater than that of all samples. The sum of the volume of the sampling liquid of the sampling bottle 6 to be detected can realize a liquid mixed detection to complete the detection of the sampling bottle 6 to be detected on the positioning and placing tray 1 of the entire sampling bottle. Assuming that the liquid volume of each sampling bottle 6 to be detected is N, the volume of liquid drawn from the sampling bottle 6 to be detected is 1/N each time, and the sampling liquid is injected into the mixing bottle. Complete the sampling of a sample bottle 6 to be tested, sterilize the automatic suction device 2 once, move the syringe of the automatic suction device 2 to the clean water pool 10, inhale clean water, transfer to the sewage pool 11 to extrude, repeat several times, and then Move to the disinfection tank 12 to inhale the disinfected water, transfer to the sewage tank to extrude, repeat several times, and finally move to the clear water tank 10, inhale clean water, transfer to the sewage tank to extrude, repeat several times, and complete the cleaning and disinfection of the automatic suction device 2.

A light source 14 is provided on one side of the camera 13 for the camera to identify and fill light; the camera 13 and the light source 14 are fixed on the rotating console 3 through a support rod 23 .

A population nucleic acid testing method based on the above-mentioned population nucleic acid testing mixing device, comprising the following steps:

Step 1), put a plurality of sampling bottles 6 numbers to be detected containing the samples to be detected into the sampling bottle placement groove 5 of the sampling bottle positioning plate 1 in turn;

Step 2), using the automatic suction device 2 to extract the sampling liquid from each sampling bottle 6 to be detected in turn, and then put it into the mixing bottle 9 to obtain the mixed sampling liquid, and put the sampling liquid in the sampling bottle 6 to be detected into the mixing bottle 9 after each completion. After inside, the automatic suction device 2 is sterilized once;

Step 3), then detect the mixed sampling liquid in the mixing bottle 9, if the test result is negative, then end this detection, if the test result is positive, then according to the middle division dyeing method to this collection of multiple to be detected. The sampling bottle 6 is divided into two half-areas during the process, and steps 1) to 3) are repeated for each half-area respectively, until the detection of the smallest half-area unit is a sampling bottle 6 to be detected, that is, the detection of multiple detection samples is completed. detection. For example, if the sampling liquid in the mixing bottle 9 is tested and the first test result is positive, then the multiple sampling bottles 6 to be tested collected this time are divided into an upper half area and a lower half area according to the middle division dyeing method. The upper half area and the lower half area are tested separately. If the upper half area is positive and the lower half area is negative, the possibility that the sample in the lower half area is positive is ruled out; the upper half area is tested by the mid-point staining method.

In this application, PCR is used to carry out the amplification test on the sampling liquid in the mixed bottle 9. Compared with the monomer test, K times of amplification are required. If the number of groups in the mixed bottle 9 is m, then K=log ₂ m, thus To make the sensitivity the same as the single-unit test, the value of the maximum number of groups m should be adjusted according to the actual achievable sensitivity, so that the test accuracy rate can meet the requirements.

As shown in Figure 4, the middle division dyeing method specifically includes the following steps:

There are TC communities or groups, the number of tests for each community is CS, and the total number of tests is:

where the total number of tests CS[i] for the i-th community is:

In formula 2, m[i] is the number of people in the i community, that is, the total number of samples. If 2 ^k-1 <m[i]<2 ^k , then m[i]=2 ^k . j represents the jth round of testing. After each round of testing, the areas without problems will be dyed with the ink mark, and these areas will not be tested in the next round. The figure shows the case of m=64, assuming that there are 5 infected samples, which are white in Figure 4, and other areas are dyed black one by one during the testing process.

As can be seen from Figure 4, in the best case, 0 cases of infection in the community only need to be tested once, if there is 1 case, it needs 2log ₂ m+1 times, while the traditional single test needs m times, with m= Taking 1024 as an example, the intermediate dyeing method requires 21 times, while the monomer test requires 1024 times. It can be seen that this method can quickly check the communities with a small number of infections, and is especially suitable for countries and regions with a small number of infections at the beginning of the infection. It can quickly check all communities with zero infections and a small number of infections. Community, and then conduct unit testing. The present invention increases the testing speed of groups with a small number of infections in a geometric progression, so that the whole city and even the whole world can be quickly checked. Compared with the development of vaccines and specific drugs, automatic control of visual recognition and amplification testing are mature technologies. The development time and accuracy are controllable, and they can be quickly controlled at the beginning of the epidemic.

Claims (10)

1. a colony nucleic acid testing mixing device, is characterized in that, comprises sampling bottle positioning and placing plate (1), mixing bottle placing plate (7), sterilizing device (8), automatic suction device (2) and for automatic suction device (2) The rotating console (3) is positioned and rotated, the sampling bottle positioning and placing plate (1), the mixing bottle placing plate (7) and the sterilizing device (8) are fixed on one side of the rotating console (3), and the sampling bottle is positioned and placed The tray (1) is provided with a plurality of sampling bottle placement grooves (5), the sampling bottles (6) to be tested are placed in the sampling bottle placement grooves (5), and the samples to be tested are stored in the sampling bottles (6) to be tested; The table (3) is provided with a mechanical arm (4) that can drive the automatic suction device (2) to rotate and move, and the sampling bottle positioning and placing plate (1), the mixing bottle placing plate (7) and the sterilizing device (8) are fixed on the automatic suction device. The device (2) moves within the range of picking and placing the liquid. 2. A kind of population nucleic acid testing mixing device according to claim 1, is characterized in that, sampling bottle placing groove (5) is arrayed on sampling bottle positioning placing plate (1), and each sampling bottle placing groove (5) There is a relative positioning position number with the rotating console (3). 3 . A population nucleic acid testing mixing device according to claim 1 , wherein a positioning and placing sleeve is provided in the sampling bottle placing groove ( 5 ). 4 . 4 . A population nucleic acid testing mixing device according to claim 3 , wherein the positioning and placing sleeve is a rubber sleeve, and the positioning and placing sleeve is adhesively connected to the groove wall of the sampling bottle placing groove ( 5 ). 5 . 5. a kind of group nucleic acid test mixing device according to claim 1 is characterized in that, disinfection device (8) comprises clear water pool (10), sewage pool (11) and disinfection pool (12), automatic suction device (2) ) can be driven into the clean water pool (10), the sewage pool (11) and the disinfection pool (12) by the mechanical arm (4). 6. a kind of colony nucleic acid testing mixing device according to claim 1, is characterized in that, automatic suction device (2) comprises needle tube (17), needle tube clamp (15) and electric push rod (16), needle tube clamp (15) ) and the electric push rod (16) are fixed on the end of the mechanical arm (4) through the fixing plate (20), the end of the piston rod (22) of the needle tube (17) is fixed on the needle tube clamp (15), the needle tube (17) A clamp (19) is fixedly clamped on the outer wall of the needle cylinder, and the clamp (19) is fixed to the end of the electric push rod (16) through a clamp rod (21). 7. A kind of colony nucleic acid testing mixing device according to claim 1, is characterized in that, the needle tube clamp (15) is provided with an opening on one side, the needle tube clamp (15) is provided with the opening and the bottom side is provided with a clamping groove, and the piston rod is provided with an opening. The end is clamped in the clamping slot, and the end of the clamping slot is locked by a screw rod (18). 8 . The population nucleic acid testing mixing device according to claim 1 , wherein the robotic arm ( 4 ) on the rotating console ( 3 ) adopts coordinate positioning or image recognition positioning. 9 . 9. A population nucleic acid testing method based on the described population nucleic acid testing mixing device of claim 1, characterized in that, comprising the following steps: Step 1), put a plurality of sampling bottle numbers to be detected containing the samples to be detected into the sampling bottle placement groove of the sampling bottle positioning plate in turn; Step 2), using the automatic suction device to extract the sampling liquid from each sampling bottle to be detected in turn, and then put it into the mixing bottle to obtain the mixed sampling liquid. The suction device is sterilized once; Step 3), then test the mixed sampling liquid in the mixing bottle, if the test result is negative, then end this test, if the test result is positive, then according to the middle-point dyeing method to collect a plurality of samples to be tested this time. The bottle 6 is divided into two half-areas during the process, and steps 1) to 3) are repeated for each half-area to detect until the smallest half-area unit is a sampling bottle to be detected, that is, the detection of multiple detection samples is completed. 10 . The population nucleic acid testing method according to claim 9 , wherein PCR is used to perform amplification testing on the sampling liquid in the mixing bottle. 11 .

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