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CN111543434A - Method for preventing and treating taro rotten taro skin disease - Google Patents

Method for preventing and treating taro rotten taro skin disease Download PDF

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Publication number
CN111543434A
CN111543434A CN202010519522.8A CN202010519522A CN111543434A CN 111543434 A CN111543434 A CN 111543434A CN 202010519522 A CN202010519522 A CN 202010519522A CN 111543434 A CN111543434 A CN 111543434A
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taro
suspending agent
collembola
trioxystrobin
fluopyram
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谢昀烨
王健康
梁俊
武军
方丽
王汉荣
盛先俏
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Zhejiang Academy of Agricultural Sciences
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Zhejiang Academy of Agricultural Sciences
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Priority to CN202110500675.2A priority patent/CN113767918A/en
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Abstract

本发明提供了一种全新的防治芋艿烂芋皮病的方法,主要采用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,进行土壤消毒、种芋处理和药液灌根,减少芋艿上的粪棘跳虫的虫口密度,从而实现对芋艿烂芋皮病的防治。本发明所述方法能更高效、用量更低、更环保地防治芋艿上粪棘跳虫的危害,降低芋艿烂芋皮病的发生,保障了农民的利益,经济社会生态效益显著。另外,本发明还公开了42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂和除虫菊提取物的混合物用与制作芋艿等农作物的粪棘跳虫防治剂的用途。The present invention provides a brand-new method for preventing and treating taro rot taro skin disease, which mainly adopts 42.8% fluobacter-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, and adds pyrethrum extract to carry out soil disinfection and planting. Taro treatment and root irrigation with liquid medicine can reduce the population density of Collembola feces on taro, so as to realize the prevention and treatment of rotten taro skin disease. The method of the invention can prevent and control the harm of Collembola feces on taro with higher efficiency, lower dosage and environmental protection, reduce the occurrence of rotten taro skin disease, ensure the interests of farmers, and have significant economic, social and ecological benefits. In addition, the present invention also discloses the use of a mixture of 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent and pyrethrum extract to make a Collembola faecalis control agent for crops such as taro.

Description

一种防治芋艿烂芋皮病的方法A kind of method for preventing and treating taro rotten taro skin disease

技术领域technical field

本发明属于植物病虫害防治领域,具体而言,涉及一种防治芋艿上的粪棘跳虫的方法。The invention belongs to the field of plant disease and insect pest control, and in particular relates to a method for preventing and treating dung beetle on taro.

背景技术Background technique

芋艿(Colocasia esculenta(L.)Schoot)简称“芋”,俗称“芋头”。原产我国和印度、马来西亚等热带地区;单子叶植物,天南星科,多年生草本。叶卵形,叶柄长而肥大,花黄绿色;地下有肉质球茎,富含淀粉,可供食用,营养丰富。其块茎含蛋白质1.75~2.3%、淀粉69.6~73.7%、灰分1.17~1.68%、脂类0.47~0.68%、钙0.059~0.169%、磷0.113~0.274%、铁0.0042~0.0050%,富含维生素B、B2(即核黄素)。《滇南本草》记载芋主宽肠胃,吞之开胃,通肠闭,滑中;久服补肝肾,添精益髓的功效。Taro (Colocasia esculenta (L.) Schoot) is referred to as "taro", commonly known as "taro". Native to my country, India, Malaysia and other tropical regions; monocotyledonous, Araceae, perennial herb. The leaves are oval, the petioles are long and hypertrophic, and the flowers are yellow-green; there are fleshy bulbs underground, which are rich in starch, edible and rich in nutrients. Its tubers contain 1.75-2.3% protein, 69.6-73.7% starch, 1.17-1.68% ash, 0.47-0.68% lipids, 0.059-0.169% calcium, 0.113-0.274% phosphorus, 0.0042-0.0050% iron and rich in vitamin B , B2 (ie riboflavin). "Southern Yunnan Materia Medica" records that taro has the effect of widening the stomach and stomach, swallowing it for appetizers, opening the intestines and closing it, and smoothing the middle; taking it for a long time has the effect of nourishing the liver and kidney, and adding essence to the marrow.

跳虫为弹尾目(Collembola)六足动物,终生无翅,体深灰色,有弹尾,体表油质,不怕水,积水时可浮在水面。跳虫以腐败的动植物残骸、腐殖质、细菌和真菌为主要食物, 可参与土壤物质循环,提高土壤肥力、改善土壤理化特性、维护土壤生物群落。跳虫种类很多,据报道可危害经济作物的跳虫有绿圆跳(Sminthurus viridis)、黄星圆跳(Bourletiellapruinosa)、紫跳(Hypogastrura comunis)、和棘跳(Onychiurus sp.)等。粪棘跳虫(Onychiurus fimeitayius)属喜湿喜腐,凡阴暗潮湿、有腐殖质存在的地方都可发现,可危害百合、天麻、芋艿等经济作物地下块茎部分,过去仅在湖南绥宁发现粪棘跳虫危害芋艿。2017年8月,在浙江省永康市也发现该危害跳虫,对芋艿地下球茎有严重危害,经调查,该危害可达到 40%以上,储藏期仍持续危害球茎,严重影响芋头的产量及价值。粪棘跳虫危害的芋艿在生长初期无明显危害症状,待芋艿生长中期危害植株可出现植株偏黄、矮小的症状,高温高湿的环境下,芋艿受害状较明显,茎干萎蔫抗病性下降,使芋田中疫病和炭疽病的更易于侵染植株,并促使芋艿植株整株腐烂,地下球茎部分全部腐烂。粪棘跳虫主要危害芋艿的地下球茎部分,成虫啃食球茎表面,形成洞槽,严重时芋头腐烂,被啃食镂空,无食用部分,完全失去生长能力,芋农称这种严重的病害为“烂芋皮”,“烂芋皮”病扩展迅速,日益严重。“烂芋皮”不仅影响产量,而且严重影响芋的外观和品质。Collembola is a hexapod of the order Collembola. It has no wings throughout its life. It has a dark gray body with a spring tail. Its body surface is oily, and it is not afraid of water. Collembola takes corrupt animal and plant remains, humus, bacteria and fungi as its main food, and can participate in soil material cycle, improve soil fertility, improve soil physical and chemical properties, and maintain soil biological communities. There are many species of springtails, and it is reported that the springtails that can harm commercial crops include green round jump (Sminthurus viridis), yellow star round jump (Bourletiellapruinosa), purple jump (Hypogastrura comunis), and spiny jump (Onychiurus sp.). Onychiurus fimeitayius is wet and rot-loving, and can be found in dark and humid places with humus. It can harm the underground tubers of commercial crops such as lily, gastrodia elata, and taro. In the past, it was only found in Suining, Hunan. Springworms harm taro. In August 2017, this harmful springtail was also found in Yongkang City, Zhejiang Province, which has serious damage to the underground bulbs of taro. After investigation, the damage can reach more than 40%, and the bulbs will continue to be harmed during the storage period, seriously affecting the yield and value of taro. . The taro that is harmed by Collembola faecalis has no obvious damage symptoms in the early stage of growth. When the taro plants are damaged in the middle stage of growth, the symptoms of yellowishness and dwarfness of the plant may appear. Under the environment of high temperature and high humidity, the damage of the taro is more obvious, and the stem is wilted and resistant to disease. The decline makes it easier for blight and anthracnose in the taro field to infect the plants, and promotes the whole taro plant to rot and the underground bulb part to rot. Collembola dungeum mainly harms the underground bulb of taro. The adults eat the surface of the bulb to form a hole. In severe cases, the taro rots and is hollowed out by the gnawing, with no edible part, and completely loses its ability to grow. Taro farmers call this serious disease as The disease of "rotten taro skin" and "rotten taro skin" spreads rapidly and becomes more and more serious. "Rotten taro skin" not only affects the yield, but also seriously affects the appearance and quality of the taro.

《芋田棘跳虫的发生特点及防治技术》(中国植保导刊,2005(8):19-20页)提到可以采用辛硫磷和高效氯氰菊酯来防治芋艿的粪棘跳虫危害,辛硫磷和高效氯氰菊酯作为广谱杀虫剂,毒性较高,对环境危害大,且容易使作物害虫产生抗药性。因此迫切需要寻找一种更高效、用量更低、更环保的防治芋艿上的粪棘跳虫的方法。"The Occurrence Characteristics and Control Technology of Collembola taro" (China Plant Protection Guide, 2005(8): 19-20) mentioned that phoxim and beta-cypermethrin can be used to prevent and control the harm of Collembola faecalis in taro, phoxim and cypermethrin. Phosphorus and beta-cypermethrin, as broad-spectrum pesticides, have high toxicity, cause great harm to the environment, and easily make crop pests resistant to pesticides. Therefore, there is an urgent need to find a more efficient, lower dosage and more environmentally friendly method for preventing and controlling Collembola feces on taro.

42.8%氟菌·肟菌酯悬浮剂(露娜森)是一种高效杀菌剂,为氟吡菌酰胺和肟菌酯的复配剂,可抑制真菌孢子萌发、芽管伸长、菌丝生长和产孢,与其他杀菌剂甚至与同类作用机理的杀菌剂也没有交互抗性。42.8%氟菌·肟菌酯悬浮剂对黄瓜靶斑病、番茄叶霉病、西瓜蔓枯病、辣椒炭疽病等多种病害有突出的防治效果。42.8% Fluorobacteria·Trioxystrobin suspending agent (Lunasen) is a high-efficiency fungicide, which is a compound of fluopyram and trioxystrobin, which can inhibit fungal spore germination, germ tube elongation, and mycelial growth. and sporulation, no cross-resistance with other fungicides or even fungicides with the same mechanism of action. 42.8% Fluorobacteria·Tristrobin suspending agent has outstanding control effect on cucumber target spot, tomato leaf mold, watermelon blight, pepper anthracnose and other diseases.

41.7%氟吡菌酰胺悬浮剂(路富达)是一种杀线虫剂,具有高效、低毒、低用量的特征,能有效解决线虫给农户带来的困扰,帮助农户生产出更高品质、更高产量的果蔬产品。41.7% Fluopyram suspending agent (Lufuda) is a nematicide with high efficiency, low toxicity and low dosage. It can effectively solve the troubles caused by nematodes to farmers and help farmers produce higher quality, Higher yield fruit and vegetable products.

除虫菊为菊属多年生草本植物,花序含除虫菊素、箭色素(jaceeidin)、芹菜素、木犀草素、芪菜素-7-半乳粮醛酸甲茎醚、芹菜素-7-葡萄糖醛酸、倍半萜内酯:[塔里定A(tatridinA)、塔里定B(taridinB)、(llR)-ll,13二氢塔里定A(llR)-11,13-dihydrotatridin-A]等,除虫菊 (Chryaanthemumcinerariaefotiumvis)全草入药制粉,可用于人体外治疥癣,也用作切花、花境供观赏,花晒干磨粉,可作杀虫药。Pyrethrum is a perennial herb of the genus Chrysanthemum. The inflorescence contains pyrethrin, jaceeidin, apigenin, luteolin, stilbene-7-galacturonate methyl ether, apigenin-7-glucuronic acid, Sesquiterpene lactones: [Tatridin A (tatridinA), Taridin B (taridinB), (llR)-ll, 13 dihydrotatridin A (llR)-11, 13-dihydrotatridin-A], etc., Pyrethrum (Chryaanthemumcinerariaefotiumvis) whole plant is used as medicine powder, which can be used to treat mange in vitro, and also used as cut flowers and flower borders for viewing.

目前还没有关于使用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,用于驱赶芋艿上的粪棘跳虫和防治芋艿烂芋皮病的相关报道。At present, there is no relevant information on the use of 42.8% fluobacter-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, and the addition of pyrethrum extract, for driving off springtails on taro and preventing and controlling taro skin disease report.

发明内容SUMMARY OF THE INVENTION

为解决上述问题,本发明提供一种更高效、用量更低、更环保的防治芋艿上的粪棘跳虫的方法,降低芋艿烂芋皮病的发生,所述方法包括以下步骤:In order to solve the above-mentioned problems, the present invention provides a more efficient, lower dosage, and more environmentally friendly method for preventing and controlling Collembola fecalis on taro, to reduce the occurrence of rotten taro skin disease, and the method comprises the following steps:

1)种植前采用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,喷洒在地面进行土壤消毒;1) Before planting, use 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, add pyrethrum extract, and spray on the ground for soil disinfection;

2)采用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,浸种芋,沥干药水后播种;2) Using 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, and adding pyrethrum extract, soaking the seed taro, draining the potion and sowing;

3)芋艿出苗或生长期,用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,灌根。3) During the emergence or growth period of taro, use 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, add pyrethrum extract, and irrigate the roots.

关于种植土壤的选择,应选择无虫源田块栽培芋头,并采用腐熟的有机肥,控制虫源进入田间,并降低田间湿度,造成不利棘跳虫生活的环境,做好田间开沟排水。Regarding the choice of planting soil, taro should be cultivated in fields without insect sources, and decomposed organic fertilizers should be used to control the entry of insect sources into the field, and reduce the humidity in the field, which will create an unfavorable environment for the living of the collembola, and the field should be ditched and drained.

关于种芋的选择,应挑选未发生过粪棘跳虫地块的芋头球茎做种芋,尽量种植早芋品种,错过跳虫重发生期;冬季挖回的种用芋头球茎在太阳下晒,经霜冻和太阳晒使粪棘跳虫若虫死亡,然后再收藏保管,最好不让种用芋头球茎在地里过冬。Regarding the selection of seed taro, the taro bulbs that have not occurred in the field of dung beetle should be selected as seed taro, and early taro varieties should be planted as much as possible, so as to miss the re-occurrence period of collembola; After frost and sun exposure, the nymphs of Collembola dunge are dead, and then they are stored for storage. It is best not to let the taro bulbs for planting spend the winter in the ground.

所述方法还可采用芋艿和水稻进行轮作。合理轮作,可减少田间虫源基数,有效防治粪棘跳虫。The method can also use taro and rice for crop rotation. Reasonable crop rotation can reduce the number of insect sources in the field, and effectively prevent and control Collembola feces.

进一步地,所述的42.8%氟菌·肟菌酯悬浮剂稀释浓度为2000倍。Further, the dilution concentration of the 42.8% fluobacterial-trioxystrobin suspending agent is 2000 times.

进一步地,所述的41.7%氟吡菌酰胺悬浮剂稀释浓度为5000倍。Further, the dilution concentration of the 41.7% fluopyram suspension agent is 5000 times.

进一步地,所述的除虫菊提取物稀释倍数为1000倍。Further, the dilution factor of the pyrethrum extract is 1000 times.

进一步地,步骤3)所述的药液灌根,所用的药液量为每亩450公斤或每株200ml。Further, step 3) described medicinal liquid irrigation root, the medicinal liquid amount used is 450 kilograms per mu or 200ml per plant.

进一步地,步骤3)所述的药液灌根时间为每年两次,第一次为种芋出苗期,第二次为芋艿4-5叶期(芋艿封行前)。Further, the described medicinal liquid root irrigation time in step 3) is twice a year, the first time is the taro seedling stage, and the second time is the 4-5 leaf stage of taro (before the taro is sealed).

进一步地,所述的药液灌根时间第一次为4月中旬,第二次为6月初。7月中旬后不建议继续使用药剂。Further, described medicinal liquid root irrigation time is mid-April for the first time, and early June for the second time. Continued use of the drug is not recommended after mid-July.

另一方面,本发明还提供了一种混合物作为农作物上粪棘跳虫防治剂的用途,所述混合物包括42.8%氟菌·肟菌酯悬浮剂2000倍液和除虫菊提取物1000倍液。On the other hand, the present invention also provides the use of a mixture as a control agent for Collembola faecalis on crops, the mixture comprising 42.8% 2,000-fold solution of fluobacterial-trioxystrobin suspension agent and 1,000-fold solution of pyrethrum extract.

由于42.8%氟菌·肟菌酯悬浮剂和除虫菊提取物对粪棘跳虫的防治作用,42.8%氟菌·肟菌酯悬浮剂和除虫菊提取物也可用于多种农作物上的粪棘跳虫的防治,如百合、天麻、芋艿等具有地下块茎部分的经济作物。Due to the control effect of 42.8% Fluorobacillus trioxystrobin suspending agent and pyrethrum extract on Collembola faecalis, 42.8% Fluorobacillus teritrilobutanil suspending agent and pyrethrum extract can also be used for Collembola faecalis on various crops control, such as lily, gastrodia elata, taro and other cash crops with underground tubers.

进一步地,所述的农作物为芋艿。Further, the crop is taro.

再一方面,本发明还提供了一种混合物作为农作物上粪棘跳虫防治剂的用途,所述混合物包括41.7%氟吡菌酰胺悬浮剂5000倍液和除虫菊提取物1000倍液。In yet another aspect, the present invention also provides the use of a mixture as a control agent for Collembola dungae on crops, the mixture comprising a 41.7% fluopyram suspension agent 5000 times and a pyrethrum extract 1000 times.

由于41.7%氟吡菌酰胺悬浮剂对粪棘跳虫的防治作用,41.7%氟吡菌酰胺悬浮剂同样也可用于多种农作物上的粪棘跳虫的防治,如百合、天麻、芋艿等具有地下块茎部分的经济作物。Due to the control effect of 41.7% fluopyram suspending agent on Collembola faecalis, 41.7% fluopyram suspending agent can also be used for the control of Collembola faecalis on various crops, such as lily, gastrodia elata, taro, etc. Cash crops of the underground tuber part.

进一步地,所述的农作物为芋艿。Further, the crop is taro.

本发明提供了一种全新的防治芋艿上的粪棘跳虫的方法,主要采用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,进行土壤消毒、种芋处理和药液灌根,并可通过芋艿和水稻进行合理轮作,减少田间虫源基数,从而实现对芋艿烂芋皮病的防治。本发明所述方法能更高效、用量更低、更环保地防治芋艿上粪棘跳虫的危害,降低芋艿烂芋皮病的发生,保障了农民的利益,经济社会生态效益显著。另外,本发明还公开了42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂和除虫菊提取物的混合物用与制作芋艿等农作物的粪棘跳虫防治剂的用途。The present invention provides a brand-new method for preventing and controlling Collembola faecalis on taro, which mainly adopts 42.8% fluobacter-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, and adds pyrethrum extract to carry out soil disinfection , seed taro treatment and root irrigation with medicinal liquid, and reasonable rotation of taro and rice can be carried out to reduce the number of insect sources in the field, so as to realize the prevention and control of taro rotten taro skin disease. The method of the invention can prevent and control the harm of Collembola feces on taro with higher efficiency, lower dosage and environmental protection, reduce the occurrence of rotten taro skin disease, ensure the interests of farmers, and have significant economic, social and ecological benefits. In addition, the present invention also discloses the use of a mixture of 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent and pyrethrum extract to make a Collembola faecalis control agent for crops such as taro.

具体实施方式Detailed ways

下面结合实施例对本发明作进一步详细描述,需要指出的是,以下所述实施例旨在便于对本发明的理解,而对其不起任何限定作用。The present invention will be described in further detail below with reference to the examples. It should be noted that the following examples are intended to facilitate the understanding of the present invention, but do not have any limiting effect on it.

实施例1 42.8%氟菌·肟菌酯悬浮剂的稀释倍数的选择Example 1 Selection of the dilution ratio of 42.8% fluobacterial-trioxystrobin suspending agent

本实施例采用42.8%氟菌·肟菌酯悬浮剂(露娜森,拜耳作物科技有限公司)和除虫菊提取物1000倍液作为芋艿的粪棘跳虫防治剂,42.8%氟菌·肟菌酯悬浮剂的稀释倍数分别为 500倍、1000倍、1500倍、2000倍、2500倍、3000倍,并采用清水作为对照;试验场地为浙江永康市曾受粪棘跳虫危害的土壤。In this example, 42.8% fluobacterial·trioxystrobin suspending agent (Lunasen, Bayer Crop Technology Co., Ltd.) and 1000 times liquid of pyrethrum extract were used as the control agent of taro collembola, 42.8% fluobacterial·trioxystrobin The dilution ratios of the suspending agent were 500 times, 1000 times, 1500 times, 2000 times, 2500 times and 3000 times, respectively, and clear water was used as a control; the test site was the soil that had been harmed by Collembola faecalis in Yongkang City, Zhejiang Province.

除虫菊提取物的制备方法为:取除虫菊花瓣,粉碎,称取1g,加入6ml 100%正己烷- 乙醇溶液,于40℃减压回流提取,过滤,得提取液,其中压强为70kPa。The preparation method of pyrethrum extract is as follows: take pyrethrum petals, pulverize, weigh 1 g, add 6 ml of 100% n-hexane-ethanol solution, extract under reduced pressure at 40° C., and filter to obtain an extract, wherein the pressure is 70 kPa.

种植前分别采用不同稀释倍数的42.8%氟菌·肟菌酯悬浮剂和除虫菊提取物1000倍液喷洒在地面进行土壤消毒;分别采用不同稀释倍数的42.8%氟菌·肟菌酯悬浮剂和除虫菊提取物1000倍液浸种芋,沥干药水后播种;芋艿出苗或生长期,分别采用不同稀释倍数的42.8%氟菌·肟菌酯悬浮剂和除虫菊提取物1000倍液灌根,亩用水量约450公斤,每株用水量约 200ml,在4月中旬种芋出苗期第一次灌根,驱赶粪棘跳虫,在6月初4-5叶期(芋艿封行前) 进行第二次灌根,8月初对芋艿地下球茎进行病虫害调查。试验过程全部采用清水作为对照。试验结果如表1所示。Before planting, 42.8% Fluorobacterial·Tristrobin suspending agent and 1000 times solution of pyrethrum extract were sprayed on the ground for soil disinfection with different dilution ratios; Seed taro was soaked in 1000 times liquid of the extract, drained and sown. During the emergence or growth period of taro, the roots were irrigated with 42.8% fluobacterial-trioxystrobin suspending agent and 1000 times liquid of pyrethrum extract with different dilution times, and the water consumption per mu was about 450 kg, water consumption per plant is about 200ml, the first time to irrigate the roots in mid-April at the emergence stage of taro, to drive off the dung beetles, and the second time to irrigate the roots at the 4-5 leaf stage in early June (before the taro is sealed) , In early August, a disease and insect pest survey was conducted on the underground bulbs of taro. During the experiment, clean water was used as the control. The test results are shown in Table 1.

表1 42.8%氟菌·肟菌酯悬浮剂的不同稀释倍数的影响Table 1 Influence of different dilution ratios of 42.8% fluobacterial-trioxystrobin suspending agent

Figure DEST_PATH_IMAGE001
Figure DEST_PATH_IMAGE001

由表1可见,采用稀释倍数为2000倍以下的42.8%氟菌·肟菌酯悬浮剂,都可以100%防治粪棘跳虫对芋艿地下块茎的危害,从环保和成本方面考虑,优选42.8%氟菌·肟菌酯悬浮剂的稀释倍数为2000倍。As can be seen from Table 1, using the 42.8% fluobacterial-trioxystrobin suspending agent with a dilution factor of less than 2000 times, all can 100% prevent and control the harm of Collembola fecalis to the underground tubers of taro, from the aspects of environmental protection and cost, preferably 42.8% The dilution ratio of fluobacterial-trioxystrobin suspending agent is 2000 times.

实施例2 41.7%氟吡菌酰胺悬浮剂的稀释倍数的选择Example 2 Selection of dilution ratio of 41.7% fluopyram suspending agent

本实施例采用41.7%氟吡菌酰胺悬浮剂(路富达,拜耳作物科学公司)和除虫菊提取物1000倍作为芋艿的粪棘跳虫防治剂,41.7%氟吡菌酰胺悬浮剂的稀释倍数分别为3000倍、3500倍、4000倍、4500倍、5000倍、5500倍、6000倍,并采用清水作为对照;试验场地为浙江永康市曾受粪棘跳虫危害的土壤。In this example, 41.7% fluopyram suspending agent (Roofuda, Bayer Crop Science Company) and 1000 times of pyrethrum extract were used as the control agent for taro collembola, and the dilution times of 41.7% fluopyram suspending agent were respectively It is 3000 times, 3500 times, 4000 times, 4500 times, 5000 times, 5500 times, and 6000 times, and clear water is used as the control;

除虫菊提取物的制备方法为:取除虫菊花瓣,粉碎,称取1g,加入6ml 100%正己烷- 乙醇溶液,于40℃减压回流提取,过滤,得提取液,其中压强为70kPa。The preparation method of pyrethrum extract is as follows: take pyrethrum petals, pulverize, weigh 1 g, add 6 ml of 100% n-hexane-ethanol solution, extract under reduced pressure at 40° C., and filter to obtain an extract, wherein the pressure is 70 kPa.

种植前分别采用不同稀释倍数的41.7%氟吡菌酰胺悬浮剂和除虫菊提取物1000倍喷洒在地面进行土壤消毒;分别采用不同稀释倍数的41.7%氟吡菌酰胺悬浮剂和除虫菊提取物 1000倍浸种芋,沥干药水后播种;芋艿出苗或生长期,分别采用不同稀释倍数的41.7%氟吡菌酰胺悬浮剂和除虫菊提取物1000倍灌根,亩用水量约450公斤,每株用水量约200ml,在4月中旬种芋出苗期第一次灌根,驱赶粪棘跳虫,在6月初4-5叶期(芋艿封行前)进行第二次灌根,8月初对芋艿地下球茎进行病虫害调查。试验过程全部采用清水作为对照。试验结果如表2所示。Before planting, 41.7% fluopyram suspending agent and 1000 times pyrethrum extract were sprayed on the ground with different dilution ratios for soil disinfection; 41.7% fluopyram suspending agent and 1000 times pyrethrum extract were used for seed soaking respectively. Taro, drain the potion and sow; taro seedling or growth period, use 41.7% fluopyram suspending agent and pyrethrum extract 1000 times with different dilution ratios to irrigate the roots, the water consumption per mu is about 450 kg, and the water consumption per plant is about 200 ml , in mid-April, irrigate the roots for the first time at the seedling stage of the taro to drive away the dung beetle, perform the second root irrigation in the 4-5 leaf stage in early June (before the taro is sealed), and carry out pest and disease damage to the underground bulbs of taro in early August. investigation. During the experiment, clean water was used as the control. The test results are shown in Table 2.

表2. 41.7%氟吡菌酰胺悬浮剂的不同稀释倍数的影响Table 2. Effects of different dilution factors of 41.7% fluopyram suspending agent

Figure DEST_PATH_IMAGE002
Figure DEST_PATH_IMAGE002

由表1可见,采用稀释倍数为5000倍以下的41.7%氟吡菌酰胺悬浮剂,都可以100%防止粪棘跳虫对芋艿的危害,降低芋艿烂芋皮病的发生,从环保和成本方面考虑,优选41.7%氟吡菌酰胺悬浮剂的稀释倍数为5000倍。As can be seen from Table 1, the 41.7% fluopyram suspending agent with a dilution ratio of less than 5000 times can be used to prevent 100% of the harm of Collembola dungeensis to taro, reduce the occurrence of taro rot taro skin disease, from the aspects of environmental protection and cost. Considering that, the dilution ratio of the 41.7% fluopyram suspending agent is preferably 5000 times.

实施例3不同药剂防治效果的比较The comparison of the control effect of embodiment 3 different medicaments

本实施例分别按照表3所示配置芋艿的粪棘跳虫防治剂,并采用清水作为对照;试验场地为浙江永康市曾受粪棘跳虫危害的土壤。供试药剂主要有:41.7%氟吡菌酰胺悬浮剂 (路富达)、42.8%氟菌·肟菌酯悬浮剂、15%茚虫威悬浮剂(彪本)、25克/升联苯菊酯乳油 (天王星)、10%虫螨腈悬浮剂(卡马特)、3.2%阿维菌素乳油(野田清快)。The present embodiment is configured according to Table 3 respectively as shown in Table 3, and uses clear water as a control; the test site is the soil of Yongkang City, Zhejiang Province, which has been harmed by Collembola faecalis. The main reagents tested are: 41.7% fluopyram suspending agent (Lufuda), 42.8% fluobacterial trioxystrobin suspending agent, 15% indoxacarb suspending agent (Biaoben), 25 g/liter bifenthrin Ester EC (Uranus), 10% chlorfenapyr suspending agent (Kamat), 3.2% Abamectin EC (Noda Qingkuai).

试验方法与实施例1一致,试验结果如表3所示。The test method is the same as that in Example 1, and the test results are shown in Table 3.

表3不同药剂防治效果的比较Table 3 Comparison of control effects of different chemicals

Figure DEST_PATH_IMAGE003
Figure DEST_PATH_IMAGE003

Figure DEST_PATH_IMAGE004
Figure DEST_PATH_IMAGE004

根据表3可以看出,比较实验组1和2,没有除虫菊提取物时,发病率仍有8.4%,加入除虫菊提取物后,可以100%防治粪棘跳虫对芋艿的危害;比较实验组3和4同样可以看出,没有除虫菊提取物时,发病率仍有8.4%,加入除虫菊提取物后,可以100%防治粪棘跳虫对芋艿的危害,可见除虫菊提取物的加入,可以大大提高防治效果;实验组5、6、7、8的发病率仍较高,其防治效果明显不如1、2、3、4组。因此,采用42.8%氟菌·肟菌酯悬浮剂2000倍液或41.7%氟吡菌酰胺悬浮剂5000倍液,并添加除虫菊提取物1000倍液配制的试剂,为最高效的芋艿粪棘跳虫防治剂。As can be seen from Table 3, comparing experimental groups 1 and 2, when there is no pyrethrum extract, the incidence rate is still 8.4%. After adding pyrethrum extract, it can 100% prevent and control the harm of Collembola faecalis to taro; Comparative experimental group 3 It can be seen from the same as 4, when there is no pyrethrum extract, the incidence rate is still 8.4%. After adding pyrethrum extract, it can 100% prevent and control the harm of Collembola feces to taro. It can be seen that the addition of pyrethrum extract can greatly improve the prevention and control. Effect; the incidence rate of experimental group 5, 6, 7, 8 is still higher, and its control effect is obviously inferior to that of group 1, 2, 3, and 4. Therefore, using 2000 times of 42.8% fluobacterial-trioxystrobin suspending agent or 5000 times of 41.7% fluopyram suspending agent and adding 1000 times of pyrethrum extract are the most efficient reagents for Collembola faecalis. control agent.

实施例4本发明处理技术对芋艿农药残留量的影响Embodiment 4 The influence of the treatment technology of the present invention on the pesticide residues of taro

同实施例3芋艿的处理,待芋艿成熟后,选取芋形正常,大小基本均匀,无明显裂痕、腐烂的芋艿进行农药残留检测。氟吡菌酰胺、肟菌酯和阿维菌素农药残留量检测按 GB/T20769-2008规定的方法进行测定,联苯菊酯农药残留量检测按GB 23200.9-2016规定的方法进行测定。Similar to the treatment of the taro in Example 3, after the taro is mature, select the taro with normal shape, basically uniform size, and no obvious cracks and rot to carry out pesticide residue detection. The pesticide residues of fluopyram, trichostrobin and abamectin were determined according to the methods specified in GB/T20769-2008, and the pesticide residues of bifenthrin were determined according to the methods specified in GB 23200.9-2016.

表4不同杀菌剂对芋艿地下块茎农药残留量的影响(mg/kg)Table 4 Effects of different fungicides on pesticide residues in taro underground tubers (mg/kg)

Figure DEST_PATH_IMAGE005
Figure DEST_PATH_IMAGE005

由表4中的结果可以看出,本发明的芋艿处理技术不仅可以有效地降低芋艿田块中的虫源基数,降低芋艿烂芋皮病的发生,而且芋艿收获期的地下块茎中并未检测到所使用的氟吡菌酰胺、肟菌酯等农药,符合中华人民共和国国家标准-水果和蔬菜中450种农药及相关化学品残留量的测定GB/T20769-2008和中华人民共和国国家标准-食品安全国家标准粮谷中475种农药及相关化学品残留量的测定GB/T20769-2008。As can be seen from the results in Table 4, the taro treatment technology of the present invention can not only effectively reduce the number of insect sources in the taro field block, reduce the occurrence of taro rotten taro skin disease, and does not detect in the underground tubers of the taro harvest period. The pesticides used, such as fluopyram and tristrobin, conform to the National Standard of the People's Republic of China-Determination of 450 pesticides and related chemicals residues in fruits and vegetables GB/T20769-2008 and the National Standard of the People's Republic of China-Food Determination of 475 pesticides and related chemicals residues in grains of national safety standards GB/T20769-2008.

实施例5本发明处理技术对芋艿安全性的影响Embodiment 5 The influence of the treatment technology of the present invention on the safety of taro

采用本发明处理技术对芋艿进行处理,待芋艿成熟后,选取芋形正常,大小基本均匀,无明显裂痕、腐烂的芋艿进行安全性检测。检测方法:食品中铅的测定根据GB/T5009.12 规定的方法进行检测,食品中镉的测定根据GB/T 5009.15规定的方法进行检测,食品中有机磷农药残留量的测定根据GB/T 5009.20规定的方法进行检测,植物性食品中辛硫磷农药残留量的测定根据GB/T 5009.102规定的方法进行检测,植物性食品中氯氰菊酯、氰戊菊酯和溴氰菊酯残留量的测定根据GB/T 5009.110规定的方法进行检测,植物性食品中三唑酮残留量的测定根据GB/T 5009.162规定的方法进行检测,蔬菜、水果中甲基托布津、多菌灵的测定根据GB/T 5009.188规定的方法进行检测。The taro is treated with the treatment technology of the invention, and after the taro is mature, the taro with normal shape, basically uniform size, and no obvious cracks and rot is selected for safety detection. Testing methods: The determination of lead in food is performed according to the method specified in GB/T5009.12, the determination of cadmium in food is performed according to the method specified in GB/T 5009.15, and the determination of organophosphorus pesticide residues in food is performed according to GB/T 5009.20 The test is carried out according to the specified method, the determination of phoxim pesticide residues in plant food is carried out according to the method specified in GB/T 5009.102, and the determination of cypermethrin, fenvalerate and deltamethrin residues in plant food is carried out according to GB /T 5009.110 for testing, determination of triazolone residues in plant foods according to GB/T 5009.162 for testing, and determination of thiophanate-methyl and carbendazim in vegetables and fruits according to GB/T 5009.188 test according to the specified method.

表5本发明处理技术对芋艿安全性检测结果Table 5 processing technology of the present invention detects the results of the safety of taro

Figure DEST_PATH_IMAGE006
Figure DEST_PATH_IMAGE006

由表5中的结果可以看出,本发明的处理技术在芋艿田块实施中有较好的安全性,在处理的芋艿中均未检出铅和镉等重金属,也未检测到乐果、敌百虫、辛硫磷、氰戊菊酯、三唑酮和多菌灵等高毒农药,符合中华人民共和国农业行业标准无公害食品芋头NY 5251-2004,其安全性较高,可以直接食用。As can be seen from the results in Table 5, the treatment technology of the present invention has better safety in the implementation of taro field block, and heavy metals such as lead and cadmium are not detected in the taro treated, nor dimethoate, Highly toxic pesticides such as trichlorfon, phoxim, fenvalerate, triazolone and carbendazim, conform to the agricultural industry standard of the People's Republic of China, pollution-free food taro NY 5251-2004, which is highly safe and can be eaten directly .

由上所述,本申请公开了芋艿生长过程中的处理方法,该处理方法对芋艿烂芋皮病具有良好得抑制作用,通过对芋艿田块中粪棘跳虫虫源基数的抑制,能够显著减少芋艿烂芋皮病的发生,从源头上改善了芋艿品质,减少了由于害虫啃食而引起病害的机会,具有安全性高,无农药残留的优点,不仅保证了芋艿的食用安全性,而且为芋艿的无公害生产提供了技术支持。From the above, the present application discloses a treatment method during the growth of taro, the treatment method has a good inhibitory effect on the rotten taro skin disease of taro. It reduces the occurrence of rotten taro skin disease, improves the quality of taro from the source, reduces the chance of disease caused by pests eating, and has the advantages of high safety and no pesticide residues, which not only ensures the food safety of taro, but also Provided technical support for the pollution-free production of taro.

虽然本发明披露如上,但本发明并非限定于此。任何本领域技术人员,在不脱离本发明的精神和范围内,均可作各种更动与修改,因此本发明的保护范围应当以权利要求所限定的范围为准。Although the present invention is disclosed above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be based on the scope defined by the claims.

Claims (10)

1.一种防治芋艿烂芋皮病的方法,减少芋艿上的粪棘跳虫的虫口密度,其特征在于,所述方法包括以下步骤:1. a method for preventing and treating rotten taro skin disease of taro, reducing the population density of Collembola dungeensis on the taro, it is characterized in that, described method may further comprise the steps: 1)种植前采用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,喷洒在地面进行土壤消毒;1) Before planting, use 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, add pyrethrum extract, and spray on the ground for soil disinfection; 2)采用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,浸种芋,沥干药水后播种;2) Using 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, and adding pyrethrum extract, soaking the seed taro, draining the potion and sowing; 3)芋艿出苗或生长期,用42.8%氟菌·肟菌酯悬浮剂或41.7%氟吡菌酰胺悬浮剂,并添加除虫菊提取物,灌根。3) During the emergence or growth period of taro, use 42.8% fluobacterial-trioxystrobin suspending agent or 41.7% fluopyram suspending agent, add pyrethrum extract, and irrigate the roots. 2.根据权利要求1所述的方法,其特征在于,所述的42.8%氟菌·肟菌酯悬浮剂稀释倍数为2000倍。2 . The method according to claim 1 , wherein the dilution ratio of the 42.8% fluobacterial-trioxystrobin suspending agent is 2000 times. 3 . 3.根据权利要求1所述的方法,其特征在于,所述的41.7%氟吡菌酰胺悬浮剂稀释倍数为5000倍。3. The method according to claim 1, wherein the dilution ratio of the 41.7% fluopyram suspending agent is 5000 times. 4.根据权利要求1-3任一项所述的方法,其特征在于,所述的除虫菊提取物稀释倍数为1000倍。4. The method according to any one of claims 1-3, wherein the dilution ratio of the pyrethrum extract is 1000 times. 5.根据权利要求1-4任一项所述的方法,其特征在于,步骤3)所述的药液灌根,所用的药液量为每亩450公斤或每株200ml。5. according to the method described in any one of claim 1-4, it is characterized in that, step 3) described medicinal liquid irrigates root, and the medicinal liquid amount used is 450 kilograms per mu or 200ml per plant. 6.根据权利要求1-5任一项所述的方法,其特征在于,步骤3)所述的药液灌根时间为每年两次,第一次为种芋出苗期,第二次为芋艿4-5叶期。6. according to the method described in any one of claim 1-5, it is characterized in that, step 3) described medicinal liquid root irrigation time is twice a year, the first time is the seedling stage of taro, and the second time is taro 4-5 leaf stage. 7.根据权利要求6所述的方法,其特征在于,所述的药液灌根时间第一次为4月中旬,第二次为6月初。7. method according to claim 6, is characterized in that, described medicinal liquid root irrigation time is mid-April for the first time, and the second time is early June. 8.一种混合物作为农作物上粪棘跳虫防治剂的用途,其特征在于,所述混合物包括42.8%氟菌·肟菌酯悬浮剂2000倍液和除虫菊提取物1000倍液。8. Use of a mixture as a control agent for Collembola faecalis on crops, characterized in that the mixture comprises a 42.8% solution of 2000-fold solution of fluobacterial-trioxystrobin suspending agent and 1,000-fold solution of pyrethrum extract. 9.一种混合物作为农作物上粪棘跳虫防治剂的用途,其特征在于,所述混合物包括41.7%氟吡菌酰胺悬浮剂5000倍液和除虫菊提取物1000倍液。9. Use of a mixture as a control agent for Collembola faecalis on crops, characterized in that the mixture comprises 5000 times the 41.7% fluopyram suspending agent and 1000 times the pyrethrum extract. 10.根据权利要求8或9所述的用途,其特征在于,所述的农作物为芋艿。10. purposes according to claim 8 or 9, is characterized in that, described crop is taro.
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