CN111961585A - Device and method for treating biomass waste by coupling rumen microbial culture with anaerobic fermentation - Google Patents
Device and method for treating biomass waste by coupling rumen microbial culture with anaerobic fermentation Download PDFInfo
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Abstract
本发明涉及生物质类废弃物厌氧发酵处理技术领域，公开了一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的装置和方法，该装置包括瘤胃微生物培养系统、厌氧发酵系统和收集系统，瘤胃微生物培养系统包括磨碎搅拌装置和监测装置，厌氧发酵系统包括搅拌装置和监测装置，瘤胃微生物培养系统与厌氧发酵系统之间上部连通，中隔壁以半透膜代替。瘤胃微生物培养系统中瘤胃微生物降解生物质类废弃物产生的各种挥发性脂肪酸(VFA)以及厌氧发酵系统中产生的缓冲物质可自由通过半透膜进行交换。进入厌氧发酵系统的VFA被产甲烷细菌利用生成甲烷，甲烷收集后可供生产生活使用；进入瘤胃微生物培养系统的缓冲物质则维持其系统pH稳定。
The invention relates to the technical field of anaerobic fermentation treatment of biomass waste, and discloses a device and method for rumen microorganism culture coupled with anaerobic fermentation to treat biomass waste. The device includes a rumen microorganism culture system, an anaerobic fermentation system and a collection method. The rumen microorganism culture system includes a grinding and stirring device and a monitoring device, and the anaerobic fermentation system includes a stirring device and a monitoring device. Various volatile fatty acids (VFAs) produced by rumen microorganisms degrading biomass-like wastes in the rumen microorganism culture system and buffer substances produced in the anaerobic fermentation system can be freely exchanged through the semipermeable membrane. The VFA entering the anaerobic fermentation system is utilized by methanogenic bacteria to generate methane, and the methane can be collected for production and living; the buffer substance entering the rumen microorganism culture system maintains the pH stability of the system.
Description
技术领域technical field
本发明提供了一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的装置和方法，属于环保微生物处理领域。The invention provides a device and method for rumen microorganism culture coupled with anaerobic fermentation to treat biomass waste, belonging to the field of environmental protection microorganism treatment.
背景技术Background technique
我国每年将生物质废弃物包括秸秆、谷壳、菜叶、餐厨垃圾、畜禽粪便等，其共同特点是富含大量纤维类物质，这部分纤维类物质属于可再生生物质资源，在目前全球能源紧张的大环境下，如何有效利用每年产生的大量纤维素类物质进行能源的转化一直是研究的热点。瘤胃微生物是栖息在反刍类动物瘤胃中的微生物总称，是迄今为止已知自然界中对纤维类物质转化效率最高的微生物菌群。利用瘤胃微生物促进生物质废物厌氧发酵，将大大提高厌氧发酵效率。研究表明，瘤胃内产生的VFA中50％～85％被瘤胃上皮所吸收，瘤胃还可分泌缓冲物质中和部分pH，还能通过生成甲烷消化部分VFA，从而维持瘤胃处于近中性状态，为瘤胃微生物营造了一个非常良好地反应环境。目前的模拟反应器并未考虑微生物降解所生成的VFA的快速处理、缓冲物质的加入等问题，只是将瘤胃微生物简单接种至反应罐，与产甲烷微生物混合发酵，不能真正有效发挥瘤胃微生物的效果。Every year, biomass waste in my country includes straw, chaff, vegetable leaves, kitchen waste, livestock and poultry manure, etc. The common feature is that it is rich in a large amount of fibrous substances, which belong to renewable biomass resources. Under the environment of global energy shortage, how to effectively utilize the large amount of cellulose materials produced every year for energy conversion has always been a research hotspot. Rumen microorganisms are the general term for the microorganisms that inhabit the rumen of ruminants, and are the microbial flora with the highest conversion efficiency to fibrous substances known to date in nature. Using rumen microorganisms to promote anaerobic fermentation of biomass waste will greatly improve the efficiency of anaerobic fermentation. Studies have shown that 50% to 85% of the VFA produced in the rumen is absorbed by the rumen epithelium, the rumen can also secrete buffer substances to neutralize part of the pH, and can also digest part of the VFA by generating methane, thereby maintaining the rumen in a near-neutral state. Rumen microbes create a very favorable environment to respond. The current simulated reactor does not consider the rapid treatment of VFA generated by microbial degradation and the addition of buffer substances, but simply inoculates rumen microorganisms into the reaction tank and mixes them with methanogenic microorganisms for fermentation, which cannot really effectively exert the effect of rumen microorganisms. .
因此，针对上述问题，本发明提供一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的装置和方法，在处理过程中，通过设备以及控制水力停留时间，将瘤胃微生物和产甲烷微生物分别置于不同的反应器内，两者之间布置的半透膜可以使VFA和缓冲物质自由流通，较好的模拟的瘤胃环境，为瘤胃微生物营造了良好地反应条件，提高了其对生物质废弃物的处理效率。Therefore, in view of the above problems, the present invention provides a device and method for rumen microorganism culture coupled with anaerobic fermentation to treat biomass waste. In different reactors, the semi-permeable membrane arranged between the two can make VFA and buffer substances circulate freely, which can better simulate the rumen environment, create good reaction conditions for rumen microorganisms, and improve their resistance to biomass waste. processing efficiency.
发明内容SUMMARY OF THE INVENTION
针对背景技术中指出的问题，本发明提出一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的装置和方法。In view of the problems pointed out in the background art, the present invention proposes a device and method for rumen microorganism culture coupled with anaerobic fermentation to treat biomass waste.
本发明的技术方案是这样实现的：The technical scheme of the present invention is realized as follows:
一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的装置，其特征在于：包括进水调节池(1)、瘤胃微生物培养池(2)、厌氧发酵池(3)和沼气收集罐(4)和接受池(5)。瘤胃微生物培养池(2)与厌氧发酵池(3)为同心圆筒，瘤胃微生物培养池(2)为内筒，厌氧发酵池(3)为外筒，内筒液面高于外筒液面，料液从瘤胃微生物培养池(2)底部流入，从筒顶溢流至厌氧发酵池(3)，筒壁(6)上半部嵌有半透膜(7)；瘤胃微生物培养池(2)内设有磨碎搅拌器(8)；厌氧发酵池(3)内设有潜水搅拌系统(9)；瘤胃微生物培养池和厌氧发酵池均设有pH、DO、VFA在线监测(10)。A device for rumen microorganism culture coupled with anaerobic fermentation to treat biomass waste, characterized in that it comprises a water inflow regulation tank (1), a rumen microorganism culture tank (2), an anaerobic fermentation tank (3) and a biogas collection tank ( 4) and the accepting pool (5). The rumen microorganism culture tank (2) and the anaerobic fermentation tank (3) are concentric cylinders, the rumen microorganism culture tank (2) is an inner cylinder, the anaerobic fermentation tank (3) is an outer cylinder, and the liquid level of the inner cylinder is higher than that of the outer cylinder At the liquid level, the feed liquid flows into the bottom of the rumen microorganism culture tank (2), overflows from the top of the cylinder to the anaerobic fermentation tank (3), and the upper half of the cylinder wall (6) is embedded with a semi-permeable membrane (7); The tank (2) is provided with a grinding agitator (8); the anaerobic fermentation tank (3) is provided with a submersible stirring system (9); both the rumen microorganism culture tank and the anaerobic fermentation tank are equipped with pH, DO, VFA online Monitoring (10).
本发明进一步设置为，所述的半透膜(7)的孔径为0.2～0.5μm，半透膜面积为筒壁面积的50～60％。According to the present invention, the pore diameter of the semipermeable membrane (7) is 0.2-0.5 μm, and the area of the semipermeable membrane is 50-60% of the area of the cylinder wall.
一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的方法，(1)驯化阶段：通过调节池，将待处理生物质废弃物泵入瘤胃微生物培养池，和厌氧发酵池，同时在厌氧发酵池内接种成熟厌氧污泥，接种量为发酵池体积的1/10～1/5。开启潜水搅拌机，按照常规厌氧驯化方法对厌氧发酵池内厌氧产沼气微生物进行驯化；A method for rumen microorganism culture coupled with anaerobic fermentation to treat biomass waste, (1) acclimation stage: through the adjustment tank, the biomass waste to be treated is pumped into the rumen microorganism culture tank and the anaerobic fermentation tank, and at the same time in the anaerobic fermentation tank. Mature anaerobic sludge is inoculated in the aerobic fermentation tank, and the inoculation amount is 1/10~1/5 of the volume of the fermentation tank. Turn on the submersible mixer and domesticate the anaerobic biogas-producing microorganisms in the anaerobic fermentation tank according to the conventional anaerobic domestication method;
当厌氧发酵池驯化完成后，在瘤胃微生物培养池内接种瘤胃内容物，接种量为发酵池体积的1/10，按池内液体体积补充玉米粉，使其终浓度不小于12g/L；开启磨碎搅拌器，将物料磨碎的同时也促进料液在瘤胃微生物培养池内循环，池内DO在线监测为0mg/L，补充碳酸氢钠，维持pH在线监测(6)为7.0±0.2mg/L，VFA在线监测为40～70mmol/L。瘤胃微生物降解生物质废弃物产生VFA，VFA可通过半透膜进入厌氧发酵池，被厌氧池内厌氧产沼气微生物利用，生成甲烷；当瘤胃微生物培养池内的VFA在线监测为55±5mmol/L，干物质的去除率达到60％以上时，即认为系统驯化成功；When the acclimation of the anaerobic fermentation tank is completed, the rumen contents are inoculated in the rumen microorganism culture tank, and the inoculation amount is 1/10 of the volume of the fermentation tank. The crusher grinds the material and also promotes the circulation of the feed liquid in the rumen microorganism culture tank. The DO online monitoring in the tank is 0 mg/L, and sodium bicarbonate is supplemented to maintain the pH online monitoring (6) at 7.0 ± 0.2 mg/L. The online monitoring of VFA is 40～70mmol/L. Rumen microorganisms degrade biomass waste to produce VFA, which can enter the anaerobic fermentation tank through the semi-permeable membrane, and be utilized by the anaerobic biogas-producing microorganisms in the anaerobic tank to generate methane; when the VFA in the rumen microorganism culture tank is monitored online at 55±5mmol/ L, when the removal rate of dry matter reaches more than 60%, it is considered that the system has been domesticated successfully;
Ⅲ.运行阶段：系统驯化成功后，采取间歇式进料，每日生物质废弃物进料体积为瘤胃微生物培养池容积的1/2。进料前1h，停止厌氧发酵池内潜水搅拌机，瘤胃微生物培养池内磨碎搅拌器正常运行。进料完成后，开启厌氧发酵池内潜水搅拌机。Ⅲ. Operation stage: After the system is successfully acclimated, intermittent feeding is adopted, and the daily feed volume of biomass waste is 1/2 of the volume of the rumen microorganism culture tank. One hour before feeding, the submersible mixer in the anaerobic fermentation tank was stopped, and the grinding mixer in the rumen microorganism culture tank was running normally. After the feeding is completed, turn on the submersible mixer in the anaerobic fermentation tank.
本发明进一步设置为，物培养耦合厌氧发酵处理生物质废弃物的方法，其特征在于其中生物质废弃物料液中生物质废弃物干物质含量为20～40g/L，生物质废弃物料液在瘤胃微生物培养池内停留时间为2d，在厌氧发酵池内停留时间为8d。。The present invention is further provided as a method for treating biomass waste by coupling anaerobic fermentation with biological culture. The residence time in the rumen microorganism culture tank was 2d, and the residence time in the anaerobic fermentation tank was 8d. .
采用了上述技术方案，本发明的有益效果为：Having adopted the above-mentioned technical scheme, the beneficial effects of the present invention are:
1、通过设置独立的瘤胃微生物培养池和厌氧发酵池，将瘤胃微生物和产甲烷微生物分开；两者之间设置半透膜，可以让瘤胃微生物培养池内产生的VFA快速扩散至厌氧发酵池内，厌氧发酵池内的碳酸盐等缓冲物质扩散进入瘤胃微生物培养池内，较好的模拟了反刍动物瘤胃的VFA吸收和pH调节功能。1. Separate rumen microorganisms and methanogenic microorganisms by setting up an independent rumen microorganism culture tank and an anaerobic fermentation tank; a semi-permeable membrane is set between the two to allow the VFA produced in the rumen microorganism culture tank to quickly diffuse into the anaerobic fermentation tank , the carbonate and other buffer substances in the anaerobic fermentation tank diffuse into the rumen microbial culture tank, which better simulates the VFA absorption and pH adjustment functions of the rumen of ruminants.
2、在瘤胃微生物培养池内设置带磨碎功能的搅拌机，既能继续磨碎有机废弃物，提高反应速率，又能促进池内物料均匀，模拟了反刍动物的咀嚼功能。2. A mixer with grinding function is installed in the rumen microorganism culture tank, which can not only continue to grind organic waste, improve the reaction rate, but also promote the uniformity of materials in the tank, simulating the chewing function of ruminants.
3、瘤胃微生物培养池停留时间为2d，符合瘤胃微生物菌群的世代时间；厌氧发酵池停留时间为8d，符合产甲烷微生物菌群的世代时间。两种不同的微生物菌群互相独立，有利于各自发挥其功能。可使料液中生物质类干物质的去除率达到60％以上，甲烷产率达到305mL/g VS，具有较好的环境效益和社会效益。3. The residence time of the rumen microbial culture tank is 2d, which is in line with the generation time of the rumen microflora; the residence time of the anaerobic fermentation tank is 8d, which is in line with the generation time of the methanogenic microflora. The two different microbial flora are independent of each other, which is conducive to each other's functions. The removal rate of biomass dry matter in the feed liquid can reach more than 60%, and the methane yield can reach 305mL/g VS, which has good environmental and social benefits.
附图说明Description of drawings
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动性的前提下，还可以根据这些附图获得其他的附图。In order to explain the embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention, and for those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.
图1为本发明一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的装置结构示意图。FIG. 1 is a schematic structural diagram of a device for rumen microorganism culture coupled with anaerobic fermentation to treat biomass waste according to the present invention.
具体实施方式Detailed ways
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
如下参考图1对本发明进行说明：The present invention will be described below with reference to Figure 1:
图1中包括有：包括进水调节池(1)、瘤胃微生物培养池(2)、厌氧发酵池(3)和沼气收集罐(4)和接受池(5)。瘤胃微生物培养池(2)与厌氧发酵池(3)为同心圆筒，瘤胃微生物培养池(2)为内筒，厌氧发酵池(3)为外筒，内筒液面高于外筒液面，料液从瘤胃微生物培养池(2)底部流入，从筒顶溢流至厌氧发酵池(3)，筒壁(6)上半部嵌有半透膜(7)；瘤胃微生物培养池(2)内设有磨碎搅拌器(8)；厌氧发酵池(3)内设有潜水搅拌系统(9)；瘤胃微生物培养池和厌氧发酵池均设有pH、DO、VFA在线监测(10)。Fig. 1 includes: including an influent adjustment tank (1), a rumen microorganism culture tank (2), an anaerobic fermentation tank (3), a biogas collection tank (4) and a receiving tank (5). The rumen microorganism culture tank (2) and the anaerobic fermentation tank (3) are concentric cylinders, the rumen microorganism culture tank (2) is an inner cylinder, the anaerobic fermentation tank (3) is an outer cylinder, and the liquid level of the inner cylinder is higher than that of the outer cylinder At the liquid level, the feed liquid flows into the bottom of the rumen microorganism culture tank (2), overflows from the top of the cylinder to the anaerobic fermentation tank (3), and the upper half of the cylinder wall (6) is embedded with a semi-permeable membrane (7); The tank (2) is provided with a grinding agitator (8); the anaerobic fermentation tank (3) is provided with a submersible stirring system (9); both the rumen microorganism culture tank and the anaerobic fermentation tank are equipped with pH, DO, VFA online Monitoring (10).
一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的装置A device for rumen microbial culture coupled with anaerobic fermentation to treat biomass waste
含有生物质废弃物的料液进入调节池(1)进行均质均量，每日进料前1h，停止厌氧发酵池(3)内潜水搅拌机，然后开启进料泵将料液从瘤胃微生物培养池(2)底部泵入池内，池内设置的磨碎搅拌器(8)一直运转，对大颗粒的生物质废弃物进行磨碎，同时使料液处于混合状态；进料时，瘤胃微生物培养池(2)内原有料液从顶部溢流进入厌氧发酵池(3)，池内原有料液溢流进入接受池(5)。进料结束后，开启厌氧发酵池(3)内设有潜水搅拌系统(9)，保证池内料液处于完全混合状态，通过在线监测(10)获得运行参数，控制反应池内状态，从而达到生物质废弃物的降解和产甲烷目的。瘤胃微生物培养池(2)和厌氧发酵池(3)内产生的沼气进入沼气收集罐(4)内暂存，处理后进行利用。瘤胃微生物培养池(2)和厌氧发酵池(3)内剩余污泥定期排出。The feed liquid containing biomass waste enters the regulating tank (1) for homogenization and homogenization. One hour before feeding every day, the submersible mixer in the anaerobic fermentation tank (3) is stopped, and then the feed pump is turned on to remove the feed liquid from the rumen microorganisms. The bottom of the culture tank (2) is pumped into the tank, and the grinding agitator (8) set in the tank keeps running to grind the large-particle biomass waste while keeping the feed liquid in a mixed state; when feeding, the rumen microorganisms are cultivated The original material liquid in the pool (2) overflows from the top into the anaerobic fermentation tank (3), and the original material liquid in the tank overflows into the receiving tank (5). After the feeding is completed, the anaerobic fermentation tank (3) is opened and a submersible stirring system (9) is provided to ensure that the material and liquid in the tank are in a completely mixed state, and the operating parameters are obtained through online monitoring (10) to control the state in the reaction tank, so as to achieve the production of raw materials. Degradation and methanogenesis of physical waste. The biogas generated in the rumen microorganism culture tank (2) and the anaerobic fermentation tank (3) enters the biogas collection tank (4) for temporary storage, and is used after treatment. The residual sludge in the rumen microorganism culture tank (2) and the anaerobic fermentation tank (3) is regularly discharged.
一种瘤胃微生物培养耦合厌氧发酵处理生物质废弃物的方法A method for rumen microbial culture coupled with anaerobic fermentation to treat biomass waste
以粉碎的稻草秸秆和牛粪为生物质废弃物，进料各项指标如下：生物质干物质浓度25～40g/L，料液COD＝4000～6000mg/L，pH＝7.0～7.5。试验系统如图1所示，反应器采用有机玻璃制作，瘤胃微生物培养池有效体积100L，厌氧发酵池有效体积400L。The crushed straw and cow dung are used as biomass waste, and the feed indicators are as follows: biomass dry matter concentration 25-40g/L, feed liquid COD=4000-6000mg/L, pH=7.0-7.5. The test system is shown in Figure 1. The reactor is made of plexiglass, the effective volume of the rumen microorganism culture tank is 100L, and the effective volume of the anaerobic fermentation tank is 400L.
具体操作如下：The specific operations are as follows:
(1)驯化阶段：准备420L料液，其中粉碎的稻草秸秆5250g(干重)，牛粪5250g(干重)，通过调节池泵入瘤胃微生物培养池和厌氧发酵池，同时在厌氧发酵池内接种市政污水处理厂厌氧发酵池内成熟厌氧污泥，接种量80L。开启潜水搅拌机，按照常规厌氧驯化方法对厌氧发酵池内厌氧产沼气微生物进行驯化。当所产沼气中甲烷浓度超过45％时，厌氧驯化成功。(1) Domestication stage: prepare 420L feed liquid, wherein 5250g (dry weight) of pulverized straw straw and 5250g (dry weight) of cow dung are pumped into the rumen microorganism culture tank and anaerobic fermentation tank through the adjustment tank, and at the same time in the anaerobic fermentation tank Mature anaerobic sludge is inoculated in the anaerobic fermentation tank of the municipal sewage treatment plant, and the inoculation volume is 80L. Turn on the submersible mixer and domesticate the anaerobic biogas-producing microorganisms in the anaerobic fermentation tank according to the conventional anaerobic domestication method. When the methane concentration in the produced biogas exceeds 45%, the anaerobic domestication is successful.
从屠宰场的牛瘤胃中取瘤胃内容物，使用2层纱布过滤后，取10L滤液加入瘤胃微生物培养池内，同时加入玉米粉1200g，开启磨碎搅拌器，维持池内DO在线监测为0mg/L，通过逐步加入碳酸氢钠维持pH在线监测为7.0±0.2mg/L，VFA在线监测为40～70mmol/L。当瘤胃微生物培养池内的VFA在线监测稳定在55±5mmol/L，取样测定料液中干物质的去除率超过60％以上时，系统驯化成功。The rumen contents were taken from the bovine rumen of the slaughterhouse, filtered with 2 layers of gauze, and 10L of the filtrate was added to the rumen microorganism culture tank, and 1200g of corn meal was added at the same time, the grinding mixer was turned on, and the DO online monitoring in the tank was maintained at 0mg/L. By gradually adding sodium bicarbonate to maintain pH online monitoring at 7.0 ± 0.2mg/L, VFA online monitoring at 40 ~ 70mmol/L. When the online monitoring of VFA in the rumen microbial culture tank is stable at 55±5mmol/L, and the removal rate of dry matter in the feed liquid is more than 60% by sampling, the system is successfully acclimated.
Ⅲ.运行阶段：系统驯化成功后，每日上午定时进料，进料量为50L，料液中粉碎的稻草秸秆750g(干重)，牛粪750g(干重)。进料前1h，首先停止厌氧发酵池内潜水搅拌机，瘤胃微生物培养池内磨碎搅拌器正常运行。进料完成后，开启厌氧发酵池内潜水搅拌机。Ⅲ. Operation stage: After the system is successfully acclimated, feed regularly every morning, with a feed volume of 50L, 750g (dry weight) of crushed straw and 750g (dry weight) of cow dung in the feed solution. 1h before feeding, first stop the submersible mixer in the anaerobic fermentation tank, and the grinding mixer in the rumen microorganism culture tank runs normally. After the feeding is completed, turn on the submersible mixer in the anaerobic fermentation tank.
正常运行期间，每天取样测定料液测TS、VS和甲烷含量，读取气体流量计的沼气产量，读取在线VFA数据。TS、VS测定按照《水和废水监测分析方法(第四版)》中方法执行，甲烷产量采取气相色谱法。选取其中连续10天数据结果见表1：During normal operation, take samples every day to measure the content of TS, VS and methane in the material and liquid, read the biogas production of the gas flow meter, and read the online VFA data. The determination of TS and VS was carried out in accordance with the method in "Water and Wastewater Monitoring and Analysis Methods (Fourth Edition)", and gas chromatography was used for methane production. The results of selecting 10 consecutive days of data are shown in Table 1:
表1检测结果Table 1 Test results
虽然以上描述了本发明的具体实施方式，但是本领域的技术人员应当理解，这些仅是举例说明，本发明的保护范围是由所附权利要求书限定的。本领域的技术人员在不背离本发明的原理和实质的前提下，可以对这些实施方式作出多种变更或修改，但这些变更和修改均落入本发明的保护范围。Although specific embodiments of the present invention have been described above, those skilled in the art will understand that these are merely illustrative and the scope of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principle and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.
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| EP0172443A1 (en) * | 1984-07-28 | 1986-02-26 | Heinz Harrendorf | Process and device for anaerobically treating organic substrates in order to produce biogas |
| US6982035B1 (en) * | 2004-03-29 | 2006-01-03 | O'keefe David M | Biphase orbicular biodigester |
| WO2010046913A2 (en) * | 2008-10-06 | 2010-04-29 | Kirloskar Integrated Technologies Ltd. | Selective filtration process for biogas production |
| WO2012099603A1 (en) * | 2011-01-21 | 2012-07-26 | The United States Of America, As Represented By The Secretary Of Agriculture | Biological/electrolytic conversion of biomass to hydrocarbons |
| CN103484502A (en) * | 2013-09-22 | 2014-01-01 | 大连理工大学 | Method for producing methane by using anerobic fermentation of rumen microorganism |
| CN105505995A (en) * | 2015-12-21 | 2016-04-20 | 湖南大学 | Method for increasing methane yield by preprocessing rice straw through rumen microorganisms |
| CN110791533A (en) * | 2019-11-14 | 2020-02-14 | 北京科技大学 | Method for improving acid production of kitchen waste saccharified residue through fermentation by using rumen microorganisms |
| CN213416888U (en) * | 2020-09-11 | 2021-06-11 | 创盛量子科技(温州)有限公司 | Device for treating biomass waste by rumen microbial culture coupling anaerobic fermentation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114273407A (en) * | 2021-12-17 | 2022-04-05 | 西安建筑科技大学 | Method for enhancing efficiency of anaerobic fermentation system by using cow dung additive |
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