CN112067409A - An improved H.E staining method for mouse bone marrow tissue sections - Google Patents
An improved H.E staining method for mouse bone marrow tissue sections Download PDFInfo
- Publication number
- CN112067409A CN112067409A CN202010782679.XA CN202010782679A CN112067409A CN 112067409 A CN112067409 A CN 112067409A CN 202010782679 A CN202010782679 A CN 202010782679A CN 112067409 A CN112067409 A CN 112067409A
- Authority
- CN
- China
- Prior art keywords
- staining
- tissue
- bone marrow
- mouse bone
- improved
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000001519 tissue Anatomy 0.000 title claims abstract description 50
- 210000001185 bone marrow Anatomy 0.000 title claims abstract description 27
- 238000007447 staining method Methods 0.000 title claims description 15
- 238000010186 staining Methods 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 11
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 10
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 5
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 abstract description 11
- 239000012188 paraffin wax Substances 0.000 abstract description 6
- 238000002791 soaking Methods 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 238000004043 dyeing Methods 0.000 abstract description 4
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 abstract description 4
- 239000000243 solution Substances 0.000 abstract description 4
- 239000012192 staining solution Substances 0.000 abstract description 4
- 238000005406 washing Methods 0.000 abstract description 4
- 210000000988 bone and bone Anatomy 0.000 abstract description 3
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 abstract description 2
- 238000004040 coloring Methods 0.000 abstract description 2
- 230000018044 dehydration Effects 0.000 abstract description 2
- 238000006297 dehydration reaction Methods 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract description 2
- 239000012153 distilled water Substances 0.000 abstract description 2
- 238000007654 immersion Methods 0.000 abstract description 2
- 239000008096 xylene Substances 0.000 abstract description 2
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 abstract 2
- 239000012670 alkaline solution Substances 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 12
- 239000011521 glass Substances 0.000 description 12
- 230000007935 neutral effect Effects 0.000 description 12
- 239000006059 cover glass Substances 0.000 description 8
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 2
- 210000002798 bone marrow cell Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- DBZJJPROPLPMSN-UHFFFAOYSA-N bromoeosin Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C(O)C(Br)=C1OC1=C(Br)C(O)=C(Br)C=C21 DBZJJPROPLPMSN-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
本发明检测方法技术领域，公开了一种改良的小鼠骨髓组织切片H.E染色方法，通过适当增加前期二甲苯脱蜡透明步骤的时长，充分将石蜡溶解，从而使得染色液在染色时充分进入组织；同时减少了酒精的浓度梯度和高浓度酒精浸泡脱水的时长，可以效防止软化后的骨组织收缩变硬而影响切片质量；在利用弱碱性溶液2％碳酸氢钠对切片进行返蓝，提高了返蓝效率和效果，且降低了成本；并且在返蓝后使用蒸馏水洗涤，有利于提高伊红溶液的染色效果。本发明经改良后的小鼠骨髓组织切片H.E染色方法合理控制组织切片在染色时的时间，使各步骤的浸泡时间更为合理，提高了骨髓组织切片着色效果，达到快速且高效的组织切片染色标准，应用前景广泛。
The technical field of the detection method of the present invention discloses an improved HE staining method for mouse bone marrow tissue sections. By appropriately increasing the duration of the pre-xylene dewaxing and clearing step, the paraffin is fully dissolved, so that the staining solution can fully enter the tissue during staining. ; At the same time, the concentration gradient of alcohol and the duration of immersion and dehydration in high-concentration alcohol are reduced, which can effectively prevent the softened bone tissue from shrinking and hardening and affecting the quality of the slices; using a weak alkaline solution of 2% sodium bicarbonate to restore the slices to blue, The blue-returning efficiency and effect are improved, and the cost is reduced; and after the blue-returning, distilled water is used for washing, which is beneficial to improve the dyeing effect of the eosin solution. The improved HE staining method for mouse bone marrow tissue slices of the present invention reasonably controls the time of tissue slice staining, makes the soaking time of each step more reasonable, improves the coloring effect of bone marrow tissue slices, and achieves fast and efficient tissue slice staining standard, with wide application prospects.
Description
技术领域technical field
本发明属于检测方法技术领域，具体涉及一种改良的小鼠骨髓组织切片H.E染色方法。The invention belongs to the technical field of detection methods, and in particular relates to an improved H.E staining method for mouse bone marrow tissue sections.
背景技术Background technique
苏木精—伊红染色法(H.Ematoxylin-eosin staining)，简称H.E染色法，是石蜡切片技术里常用的染色法之一。苏木精染液为碱性，主要使细胞核内的染色质与胞质内的核酸着紫蓝色；伊红为酸性染料，主要使细胞质和细胞外基质中的成分着红色。Hematoxylin-eosin staining (H.Ematoxylin-eosin staining), referred to as H.E staining, is one of the commonly used staining methods in paraffin section technology. The hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm violet blue; eosin is an acidic dye, which mainly makes the components in the cytoplasm and the extracellular matrix red.
在病理组织学观察中，常常需要对小鼠的骨髓进行病理切片，以便于观察其疾病或药物对骨髓组织结构、骨髓细胞形态与数量等的影响；H.E染色法在组织切片中使用最广泛，利用碱性苏木素和酸性伊红两种染液对石蜡切片进行染色，染色的步骤与染液浸泡的时间决定了染色的质量，对后期切片观察中的清晰程度有很大的影响，决定了组织切片的质量。In histopathological observation, it is often necessary to perform pathological sections on the bone marrow of mice, so as to observe the effects of diseases or drugs on bone marrow tissue structure, bone marrow cell morphology and number, etc. H.E staining is the most widely used in tissue sections. The paraffin sections are stained with alkaline hematoxylin and acid eosin. The staining steps and the soaking time in the staining solution determine the quality of the staining, which has a great influence on the clarity of the later section observation and determines the tissue. The quality of the slices.
在进行H.E染色时，操作人员应当密切关注试剂浓度改变和浸泡时间，并依据实际操作需求，合理控制温度、湿度环境，确保各项操作的合理、规范、科学。切片组织结构较为复杂，试验员在进行染色的过程中，应当控制好染色时间，同时保证染色剂能够和组织充分结合，确保各组织均可实现彻底染色。When carrying out H.E staining, operators should pay close attention to the change of reagent concentration and soaking time, and reasonably control the temperature and humidity environment according to the actual operation requirements, so as to ensure the reasonable, standardized and scientific operation of each operation. The tissue structure of the slice is relatively complex. During the staining process, the experimenter should control the staining time, and at the same time ensure that the dye can be fully combined with the tissue to ensure that each tissue can be completely stained.
标准的H.E染色包括以下步骤：Standard H.E staining involves the following steps:
(20)中性树胶封片：用玻璃棒蘸中性树胶，滴在载玻片上组织边缘，镊子夹起载玻片边缘，将盖玻片轻轻盖住组织，使中性树胶在盖玻片下自由扩散，覆盖组织即可。(20) Neutral gum sealing: dip a glass rod in neutral gum, drop it on the edge of the tissue on the glass slide, pick up the edge of the glass slide with tweezers, and gently cover the tissue with the cover glass so that the neutral gum is on the cover glass. Spread freely under the slice and cover the tissue.
但由于苏木素浸泡染色时间与染色后的流水冲洗时长、伊红浸泡染色时间与染色后乙醇清洗时长都难以把握，过长过短都会对细胞着色造成很大的影响，细胞着色效果差不利于组织切片的观察，并且高浓度酒精会使脱钙软化的骨组织收缩变硬。However, it is difficult to grasp the time of hematoxylin soaking and staining, the length of running water washing after staining, the time of eosin soaking and staining and the length of ethanol washing after staining. Too long or too short will have a great impact on cell staining, and poor cell staining effect is not conducive to tissue. The observation of slices, and the high concentration of alcohol will make the decalcified softened bone tissue shrink and harden.
发明内容SUMMARY OF THE INVENTION
本发明提出一种改良的小鼠骨髓组织切片H.E染色方法，以解决现有技术中存在的一个或多个技术问题，至少提供一种有益的选择或创造条件。The present invention proposes an improved H.E staining method for mouse bone marrow tissue sections, so as to solve one or more technical problems existing in the prior art, and at least provide a beneficial choice or create conditions.
为了克服上述技术问题，本发明采用的技术方案如下：In order to overcome the above-mentioned technical problems, the technical scheme adopted in the present invention is as follows:
一种改良的小鼠骨髓组织切片H.E染色方法，所述小鼠骨髓组织切片H.E染色方法中使用了碳酸氢钠溶液进行处理。An improved H.E staining method for mouse bone marrow tissue sections, wherein sodium bicarbonate solution is used for processing in the H.E staining method for mouse bone marrow tissue sections.
作为上述方案的进一步改进，所述小鼠骨髓组织切片H.E染色方法中先用2％的碳酸氢钠溶液进行浸泡，然后用流水冲洗。As a further improvement of the above-mentioned scheme, in the H.E staining method of the mouse bone marrow tissue section, first soak in 2% sodium bicarbonate solution, and then rinse with running water.
作为上述方案的进一步改进，所述小鼠骨髓组织切片H.E染色方法中还用到了50％-100％的酒精。As a further improvement of the above scheme, 50%-100% alcohol is also used in the H.E staining method of the mouse bone marrow tissue section.
作为上述方案的进一步改进，所述小鼠骨髓组织切片H.E染色方法中还用到了70％-100％的酒精。As a further improvement of the above scheme, 70%-100% alcohol is also used in the H.E staining method of the mouse bone marrow tissue section.
作为上述方案的进一步改进，所述小鼠骨髓组织切片H.E染色方法包括以下步骤：As a further improvement of the above scheme, the H.E staining method of the mouse bone marrow tissue section comprises the following steps:
(19)中性树胶封片：用玻璃棒蘸中性树胶，滴在载玻片上组织边缘，镊子夹起载玻片边缘，将盖玻片轻轻盖住组织，使中性树胶在盖玻片下自由扩散，覆盖组织即可。(19) Sealing with neutral gum: dip a glass rod in neutral gum, drop it on the edge of the tissue on the glass slide, pick up the edge of the glass slide with tweezers, and gently cover the tissue with the cover glass so that the neutral gum is on the cover glass. Spread freely under the slice and cover the tissue.
本发明的有益效果是：本发明提供了一种改良的小鼠骨髓组织切片H.E染色方法，通过适当增加前期二甲苯脱蜡透明步骤的时长，充分将石蜡溶解，从而使得染色液在染色时充分进入组织；同时减少了酒精的浓度梯度和高浓度酒精浸泡脱水的时长，可以效防止软化后的骨组织收缩变硬而影响切片质量；在利用弱碱性溶液2％碳酸氢钠对切片进行返蓝，提高了返蓝效率和效果，且降低了成本；并且在返蓝后使用蒸馏水洗涤，有利于提高伊红溶液的染色效果。本发明经改良后的小鼠骨髓组织切片H.E染色方法合理控制组织切片在染色时的时间，使各步骤的浸泡时间更为合理，提高了骨髓组织切片着色效果，达到快速且高效的组织切片染色标准，有利于观察骨髓细胞的变化和后纪对疾病发展的分析。该染色方法成本更低、耗材更少、染色效果增强、也更快捷合理更有效率，应用前景广泛。The beneficial effects of the present invention are as follows: the present invention provides an improved H.E staining method for mouse bone marrow tissue slices. By appropriately increasing the duration of the pre-xylene dewaxing and clearing step, the paraffin can be fully dissolved, so that the staining solution can be fully dissolved during staining. Enter the tissue; at the same time, the concentration gradient of alcohol and the duration of immersion and dehydration in high-concentration alcohol are reduced, which can effectively prevent the softened bone tissue from shrinking and hardening and affecting the quality of the slice; Blue, improve the efficiency and effect of returning to blue, and reduce the cost; and washing with distilled water after returning to blue is beneficial to improve the dyeing effect of the eosin solution. The improved H.E staining method for mouse bone marrow tissue slices of the present invention reasonably controls the time of tissue slice staining, makes the soaking time of each step more reasonable, improves the coloring effect of bone marrow tissue slices, and achieves fast and efficient tissue slice staining Standard, it is beneficial to observe the changes of bone marrow cells and the analysis of disease development in the post-mortem. The dyeing method has lower cost, fewer consumables, enhanced dyeing effect, quicker, more reasonable and more efficient, and has wide application prospects.
附图说明Description of drawings
图1是实施例1所得的在经改良H.E染色方法进行染色的小鼠骨髓切片染色图，该图的放大比例为100倍；Fig. 1 is the staining diagram of the mouse bone marrow section obtained by the modified H.E staining method obtained in Example 1, and the magnification ratio of this diagram is 100 times;
图2是对比例1所得的在经常规H.E染色方法进行染色的小鼠骨髓切片染色图，该图的放大比例为100倍。FIG. 2 is a staining diagram of the mouse bone marrow section obtained by the conventional H.E staining method obtained in Comparative Example 1, and the magnification ratio of the diagram is 100 times.
具体实施方式Detailed ways
下面结合实施例对本发明进行具体描述，以便于所属技术领域的人员对本发明的理解。有必要在此特别指出的是，实施例只是用于对本发明做进一步说明，不能理解为对本发明保护范围的限制，所属领域技术熟练人员，根据上述发明内容对本发明所作出的非本质性的改进和调整，应仍属于本发明的保护范围。同时，下述所提及的原料未详细说明的，均为市售产品；未详细提及的工艺步骤或提取方法为均为本领域技术人员所知晓的工艺步骤或提取方法。The present invention will be specifically described below with reference to the embodiments, so as to facilitate the understanding of the present invention by those skilled in the art. It is necessary to point out that the embodiments are only used to further illustrate the present invention, and should not be construed as limiting the scope of protection of the present invention. Those skilled in the art make non-essential improvements to the present invention according to the above-mentioned content of the invention. and adjustment, should still belong to the protection scope of the present invention. Meanwhile, the raw materials mentioned below are all commercially available products that are not described in detail; the process steps or extraction methods not mentioned in detail are the process steps or extraction methods known to those skilled in the art.
实施例1Example 1
采用改良H.E染色法对鸡法氏囊石蜡样本进行染色。将样本切片处理后按以下步骤进行染色：Chicken bursal paraffin samples were stained using modified H.E staining. After processing the sample sections, stain as follows:
(19)中性树胶封片：用玻璃棒蘸中性树胶，滴在载玻片上组织边缘，镊子夹起载玻片边缘，将盖玻片轻轻盖住组织，使中性树胶在盖玻片下自由扩散，覆盖组织即可。(19) Sealing with neutral gum: dip a glass rod in neutral gum, drop it on the edge of the tissue on the glass slide, pick up the edge of the glass slide with tweezers, and gently cover the tissue with the cover glass so that the neutral gum is on the cover glass. Spread freely under the slice and cover the tissue.
制得如图1所示的染色片，从图1可以看出，组织的染色程度深，对比度明显，染色效果好。The stained sheet shown in Fig. 1 was obtained, and it can be seen from Fig. 1 that the staining degree of the tissue was deep, the contrast was obvious, and the staining effect was good.
对比例1Comparative Example 1
取与实施例1同一鸡法氏囊石蜡样本进行标准H.E染色：具体包括以下步骤：Take the same chicken bursal paraffin sample as in Example 1 for standard H.E staining: specifically include the following steps:
(20)中性树胶封片：用玻璃棒蘸中性树胶，滴在载玻片上组织边缘，镊子夹起载玻片边缘，将盖玻片轻轻盖住组织，使中性树胶在盖玻片下自由扩散，覆盖组织即可。(20) Neutral gum sealing: dip a glass rod in neutral gum, drop it on the edge of the tissue on the glass slide, pick up the edge of the glass slide with tweezers, and gently cover the tissue with the cover glass so that the neutral gum is on the cover glass. Spread freely under the slice and cover the tissue.
制得如图2所示的染色片。从图2可以看出，组织的染色浅，对比度较差，染色效果比不理想。The stained sheet shown in Figure 2 was produced. It can be seen from Figure 2 that the staining of the tissue is light, the contrast is poor, and the staining effect is not ideal.
对于本发明所属技术领域的普通技术人员来说，在不脱离本发明构思的前提下还可以做出若干简单推演或替换，而不必经过创造性的劳动。因此，本领域技术人员根据本发明的揭示，对本发明做出的简单改进都应该在本发明的保护范围之内。上述实施例为本发明的优选实施例，凡与本发明类似的工艺及所作的等效变化，均应属于本发明的保护范畴。For those of ordinary skill in the technical field to which the present invention pertains, some simple deductions or substitutions can be made without departing from the concept of the present invention without creative work. Therefore, simple improvements made to the present invention by those skilled in the art according to the disclosure of the present invention should all fall within the protection scope of the present invention. The above-mentioned embodiments are preferred embodiments of the present invention, and all processes similar to those of the present invention and equivalent changes made shall belong to the protection scope of the present invention.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010782679.XA CN112067409A (en) | 2020-08-06 | 2020-08-06 | An improved H.E staining method for mouse bone marrow tissue sections |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010782679.XA CN112067409A (en) | 2020-08-06 | 2020-08-06 | An improved H.E staining method for mouse bone marrow tissue sections |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112067409A true CN112067409A (en) | 2020-12-11 |
Family
ID=73657153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010782679.XA Pending CN112067409A (en) | 2020-08-06 | 2020-08-06 | An improved H.E staining method for mouse bone marrow tissue sections |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112067409A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090246824A1 (en) * | 2008-03-27 | 2009-10-01 | Richard-Allan Scientific Company | Methods for Integrated Tissue Processing and Staining |
| CN105067412A (en) * | 2015-08-10 | 2015-11-18 | 长春瑞克医疗科技有限公司 | Vaginal secretion staining fluid, and preparation method and staining method thereof |
| CN110686957A (en) * | 2019-10-11 | 2020-01-14 | 佛山科学技术学院 | Improved HE dyeing method |
-
2020
- 2020-08-06 CN CN202010782679.XA patent/CN112067409A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090246824A1 (en) * | 2008-03-27 | 2009-10-01 | Richard-Allan Scientific Company | Methods for Integrated Tissue Processing and Staining |
| CN105067412A (en) * | 2015-08-10 | 2015-11-18 | 长春瑞克医疗科技有限公司 | Vaginal secretion staining fluid, and preparation method and staining method thereof |
| CN110686957A (en) * | 2019-10-11 | 2020-01-14 | 佛山科学技术学院 | Improved HE dyeing method |
Non-Patent Citations (1)
| Title |
|---|
| 方福德: "现代医学实验技巧全书 上", 北京医科大学 中国协和医科大学联合出版社国协和医科大学联合出版社国协和医科大学联合出版社, pages: 28 - 29 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107490511B (en) | A kind of haematoxylin dyeing liquid and HE colouring method | |
| JP2022009735A5 (en) | ||
| CN103940648B (en) | The preparation method of fish gill tissue paraffin section | |
| CN102876081B (en) | Hematoxylin staining solution and papanicolaou staining kit containing same | |
| CN104374601B (en) | A kind of resin slicer preparation method of kernel maturity seed | |
| CN105651580A (en) | Hematoxylin-eosin mixed staining solution | |
| CN107917832A (en) | The production method of fish ovary tissue paraffin section | |
| CN110672395A (en) | HE staining method for tissue section | |
| CN107702955B (en) | Preparation method of paraffin section | |
| CN112014192A (en) | HE staining method for paraffin section of northern Guizhou goat ovary tissue | |
| CN110686957A (en) | Improved HE dyeing method | |
| CN107271233A (en) | A kind of processing method of histological tissue specimen | |
| CN107576552B (en) | Paraffin section dyeing method for observing infection process of China rose black spot | |
| CN112067409A (en) | An improved H.E staining method for mouse bone marrow tissue sections | |
| CN119935694A (en) | A bone tissue multiple special staining analysis method and its application | |
| CN111896353A (en) | A kind of dehydration method of small tissue block | |
| CN115266271A (en) | Demelanization solution and application, method of demelanization and application and dyeing method | |
| CN107271241B (en) | A kind of frozen section method of Gorky's silver staining nerve fiber | |
| CN110132673A (en) | A kind of method for preparing slices of fruit body tissue of Flammulina velutipes | |
| CN1975367A (en) | Tissue paraffin section de-waxing method | |
| CN111829859B (en) | Efficient poplar seed transparent dyeing and three-dimensional imaging method thereof | |
| CN109900537A (en) | A kind of processing method of histological tissue specimen | |
| CN117286136A (en) | Dewaxing liquid for extracting paraffin tissue from nucleic acid | |
| CN107941587A (en) | A kind of method that improved Golgi Cox dyeing prepares brain tissue paraffin section | |
| CN105259001B (en) | A kind of paraffin section pre-treating method for tissue biopsy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Country or region after: China Address after: No.33 Guangyun Road, Shishan town, Nanhai District, Foshan City, Guangdong Province Applicant after: Foshan University Address before: No.33 Guangyun Road, Shishan town, Nanhai District, Foshan City, Guangdong Province Applicant before: FOSHAN University Country or region before: China |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201211 |