CN112197994A - Full-automatic vibrations formula slicer - Google Patents
Full-automatic vibrations formula slicer Download PDFInfo
- Publication number
- CN112197994A CN112197994A CN202011113019.9A CN202011113019A CN112197994A CN 112197994 A CN112197994 A CN 112197994A CN 202011113019 A CN202011113019 A CN 202011113019A CN 112197994 A CN112197994 A CN 112197994A
- Authority
- CN
- China
- Prior art keywords
- axis
- shaft assembly
- guide rail
- connecting plate
- block
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B19/00—Programme-control systems
- G05B19/02—Programme-control systems electric
- G05B19/04—Programme control other than numerical control, i.e. in sequence controllers or logic controllers
- G05B19/042—Programme control other than numerical control, i.e. in sequence controllers or logic controllers using digital processors
- G05B19/0423—Input/output
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
- G01N2001/065—Drive details
- G01N2001/066—Drive details electric
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a full-automatic vibrating slicer, which comprises a base, a sample box, a Y shaft assembly, an X shaft assembly and a Z shaft assembly, wherein the X shaft assembly and the Z shaft assembly are arranged on the base; the sample cartridge is arranged on the X shaft assembly; the Y shaft assembly is arranged on the Z shaft assembly; the Y-axis assembly, the sample cartridge, and the X-axis assembly are arranged from top to bottom. The thickness of each piece of tissue cutting is automatically and accurately controlled through an X-axis stepping motor, a Z-axis stepping motor and a linear sliding module, and errors caused by manual operation are eliminated; the direct current servo motor of the Y shaft and the eccentric wheel form a mechanism for converting circular motion into linear reciprocating motion, so that the noise generated by the high-speed motion of the Y shaft and the possibility of increasing the clearance after friction generated by long-term reciprocating motion are effectively reduced; the operation of the whole machine is controlled by adopting the embedded microcontroller, so that the switching of functions such as full-automatic processing, manual processing and the like is realized.
Description
Technical Field
The invention relates to the technical field of life science experimental instruments, in particular to a full-automatic vibrating type slicing machine.
Background
In the field of biological and life science experiments, microtomes play an important role, mainly manifested by the following two applications: one is paraffin and ultra-thin section of frozen tissue for embedding, and the section is used for morphological study of the tissue; another section for fresh biological tissue is used for tissue cell culture. Tissue cell culture is an experimental method frequently applied to research in the fields of life science and medicine, and there are two types of cell culture and tissue mass culture. Wherein, the tissue slice of animal tissue culture is mainly applied to brain slice culture in neuroscience research.
Most of slicing machines on the market at present belong to a semi-automatic type, the movement in the X-axis direction is controlled by a direct-current servo motor to carry out processing and cutting, and the processing thickness of a tissue slice is adjusted in the Z-axis direction by a screw rod and dovetail groove mechanical structure. Cutting a slice at every turn and needing the thickness of artifical manual adjustment next cutting, both consuming time like this and also hard, also can cause simultaneously because the artifical various errors that manual adjustment thickness brought.
Disclosure of Invention
Based on the technical scheme, the invention provides a full-automatic vibrating slicer, and aims to solve the problem of precision error of manually adjusting the cutting thickness.
In order to achieve the purpose, the invention provides the following technical scheme:
a full-automatic vibrating slicer comprises a base, a sample box, a Y-axis assembly, an X-axis assembly and a Z-axis assembly, wherein the X-axis assembly and the Z-axis assembly are arranged on the base; the sample cartridge is arranged on the X shaft assembly; the Y shaft assembly is arranged on the Z shaft assembly; the Y shaft assembly, the sample box and the X shaft assembly are arranged from top to bottom; the Z-axis assembly comprises a Z-axis shell, a support, a Z-axis stepping motor, a Z-axis screw rod, a Z-axis guide rail, a first connecting plate, a second connecting plate, a Z-axis sliding block connected with the Z-axis guide rail in a sliding manner, and a limiting support arranged on the support; the support is arranged on the inner side wall of the Z-axis shell, and the Z-axis guide rail is arranged on the side surface of the support; the first connecting plate and the second connecting plate are respectively arranged at two ends of the Z-axis guide rail; the Z-axis stepping motor is arranged at one end, close to the base, of the second connecting plate; one end of the Z-axis screw rod is connected to the Z-axis stepping motor, and the other end of the Z-axis screw rod penetrates through the Z-axis sliding block and is connected to the first connecting plate; the Z-axis stepping motor accurately controls the Z-axis sliding block to slide on the Z-axis guide rail, so that the slicing machine can automatically adjust the cutting thickness, reduce errors and improve the slicing quality; compared with the prior art, the semi-automatic slicing machine overcomes the defects that the existing semi-automatic slicing machine consumes time and labor and is inconsistent in cutting precision.
Furthermore, the limiting bracket comprises a first limiting bracket and a second limiting bracket arranged opposite to the first limiting bracket; the Z-axis sliding block can reciprocate between the first limit bracket and the second limit bracket; when the Z-axis sliding block starts to slide at a set initial position close to one side of the first limiting bracket and reaches the position of the second limiting bracket, the Z-axis sliding block moves reversely immediately and automatically retreats to the initial position.
Furthermore, one end of the Z-axis sliding block is connected with the Y-axis assembly, and the Y-axis assembly is driven by the Z-axis sliding block to move in the Z-axis direction.
Furthermore, the Y-axis assembly comprises a bracket, a Y-axis direct-current servo motor, an eccentric wheel, an axle pin, a Y-axis guide rail, a Y-axis sliding block, a connecting block, a cutter arm, a blade seat and a cutter pressing block; the Y-axis direct current servo motor is arranged on the side face of the bracket; the Y-axis guide rail is arranged on the side surface of the bracket far away from the Y-axis direct current servo motor; one end of the bracket, which is close to the Y-axis direct current servo motor, is connected with the Z-axis sliding block; the Y-axis guide rail is connected with the Y-axis sliding block in a sliding manner; one side of the connecting block is connected with the sliding block, and the other side of the connecting block is connected with the cutter arm; one end of the eccentric wheel is connected with the Y-axis direct current servo motor, and the other end of the eccentric wheel is connected with the connecting block through the shaft pin; the blade seat is arranged on the cutter arm; the knife pressing block is arranged on the blade seat. The vibration of the Y-axis component uses the Y-axis direct-current servo motor and an eccentric wheel structure to convert circular motion into linear reciprocating motion; the noise generated by the Y-axis component during high-speed motion and the possibility of increasing the clearance after friction generated by long-term reciprocating motion are effectively reduced.
Furthermore, the Y-axis direct current servo motor is connected with the eccentric wheel, converts the circular motion of the Y-axis direct current servo motor into linear reciprocating motion, and drives the Y-axis sliding block to reciprocate along the Y-axis guide rail; one side, away from the direct current servo motor, of the eccentric wheel is provided with a first elastic check ring, and the first elastic check ring can effectively guarantee the stability of connection of the direct current servo motor and the eccentric wheel.
Furthermore, a gasket is arranged between the eccentric wheel and the connecting block, so that the eccentric wheel and the connecting block can be effectively prevented from being abraded by high pressure generated in high-speed motion; in addition, one end, close to the connecting block, of the shaft pin is provided with a second retaining ring, and the second retaining ring can effectively fasten the axial movement of the shaft pin, so that the stability of the structure is guaranteed.
Further, a first groove is formed in the blade seat, and the cutter pressing block is arranged in the first groove; when the blade is installed, the blade pressing block presses the blade, the blade is adjusted to a proper position, and the hand-screwed screw is screwed tightly to complete the fixation of the blade; the position and the angle of the blade can be adjusted by loosening the hand-screwed screw according to the needs in the use process.
Furthermore, the X-axis assembly comprises an X-axis shell, a front cover plate, a groove cover plate, an X-axis sliding block, an X-axis guide rail, a third connecting plate, a fourth connecting plate, a limiting block, an X-axis stepping motor, an X-axis lead screw and a sensor; the X guide rail and the sensor are respectively arranged on the base; one end of the X-axis guide rail is connected with the third connecting plate, and the other end of the X-axis guide rail is connected with the fourth connecting plate; the X-axis stepping motor is arranged on one side, far away from the X-axis guide rail, of the fourth connecting plate; one end of the X-axis lead screw is connected to the X-axis stepping motor, and the other end of the X-axis lead screw penetrates through the X-axis sliding block and is connected to the third connecting plate; the third connecting plate is arranged on the inner side wall of the X-axis shell; the X-axis sliding block is connected with the X-axis guide rail in a sliding manner; the groove cover plate is arranged on the X-axis sliding block;
furthermore, the limiting block comprises a forward limiting block and a backward limiting block which are arranged on the same side surface of the groove cover plate; through setting up advance stopper with back down the stopper, control effectively advance and back down of X axle subassembly accomplish the section action.
The fixed screw holes arranged on the advancing limiting block and the backing limiting block are provided with a movable distance of 4 cm; before slicing, fixing the advancing limiting block and the backing limiting block at the middle position of the fixed screw hole through hand-screwed screws; during slicing, moving the forward limiting block to one side of the backspace limiting block by 2cm, moving the backspace limiting block to one side of the forward limiting block by 2cm, and fixing the forward limiting block by screwing a screw by hand, so that a tissue slice with the maximum length of 4cm can be actually cut, and the distance is far greater than the normal tissue slice area; in the slicing process, the distance between the advancing limiting block and the retreating limiting block can be adjusted according to actual needs, and then the distance of the tissue slice area is adjusted.
Further, the sensor includes a first sensor and a second sensor; the distance between the first sensor and the second sensor is larger than the distance between the advancing limiting block and the backing limiting block.
When tissue slicing is started, the X-axis assembly is in an initial state, the forward limiting block corresponds to the first sensor, the backward limiting block is arranged between the first sensor and the second sensor, the X-axis sliding block starts to move from one side, close to the second sensor, of the first sensor to one side of the second sensor under the driving of the X-axis stepping motor, and then the tissue is sliced for the first time; when the backspacing limiting block reaches the second sensor, the X slide block immediately changes the motion direction and backs towards one side of the first sensor; when the advancing limiting block reaches the first sensor, the Z-axis stepping motor moves downwards for a set distance according to the set slice thickness, and meanwhile, the X-axis sliding block changes the movement direction again and moves towards one side of the second sensor to process the tissue slices, and the process is repeated until the slicing action is completed.
In the invention, the X-axis assembly and the Z-axis assembly are all changed into a linear sliding block controlled by a stepping motor to carry out slicing processing, so that a dovetail groove processing process of a semi-automatic slicer in the prior art is omitted. The microtome of the present invention is essentially cost-consistent with a semi-automatic microtome.
Furthermore, a sliding groove is formed in the groove cover plate, and the sliding groove is connected with the sample box in a sliding mode and can be fixed through hand-screwed screws.
Further, the sample box comprises a bottom box, a circular truncated cone and a U-shaped card arranged on the bottom box; the processing round table is arranged on the U-shaped clamp through a screw; a boss is arranged at one end of the bottom box, and a second groove is arranged at the other end of the bottom box; the boss is matched with the sliding groove in a sliding manner; the U-shaped card is arranged in the second groove.
Specifically, when slicing, firstly, adding a tissue culture solution into the second groove, then fixing the tissue to be sliced on the circular truncated cone by using glue, secondly, adjusting the U-shaped clamp to a proper position, then, screwing down a hand screw, and finally, adjusting the position of the bottom box on the groove cover plate to complete slicing action by matching with the X-axis assembly and the Y-axis assembly.
Fig. 8 shows a schematic diagram of a control circuit of a full-automatic vibrating slicer according to the present invention, in this embodiment, the control system of the full-automatic vibrating slicer includes an embedded microcontroller 1, external key input interface circuits 2 connected to the embedded microcontroller 1, an X-axis and Z-axis driving and direction control circuit 3, a Y-axis vibration control motor driving circuit 4, and an X-axis, Y-axis and Z-axis display driving circuit 5; and an X-axis, Y-axis, Z-axis speed adjusting circuit 6; the slicer of the invention adopts an embedded microcontroller 1 to control the operation of the whole slicer; the Y-axis direct current servo motor 302 utilizes the PWM function of the embedded microcontroller 1, and then the Y-axis vibration control motor driving circuit 4 and the Y-axis speed adjusting circuit function to regulate the speed of the vibration motor; under the regulation of the speed regulating circuits of the X axis and the Z axis, the control motors of the X axis and the Z axis are controlled by adopting stepping motors, and the stepping motors drive the screw rods to rotate; the main control board controls the power amplifier of the stepping motor through the pulse and I/O output of the embedded microcontroller 1, under the action of the X-axis and Z-axis drive and direction control circuit 3, the power amplifier of the stepping motor drives the stepping motor to rotate forwards and backwards to process slices, and the processing speed and thickness can be adjusted through the microcontroller. The screw rod advances by 1mm every time the stepping motor rotates for one circle; each pulse goes 1.8 °; therefore, 200 pulses need to be sent when the X axis and the Z axis advance for 1 mm; the invention divides the drivers of the X-axis stepping motor 409 and the Z-axis stepping motor 53 into four parts, and 800 pulses are required to be sent every 1mm of forward movement. In the technical scheme provided by the invention, the control resolution of the slicer is 1 mm/800-0.00125 mm (namely 1.25um), namely the thickness of the slice which can be controlled by the slicer is 1.25um at the minimum. The processing speed and the cutting thickness can be displayed by driving the LED display screen through the X-axis, Y-axis and Z-axis display driving circuits 5, and the speed and the thickness can be adjusted by the microcontroller through the adjusting buttons of the panel.
The embedded microcontroller technology is adopted for controlling the machine, so that the slicing machine can be flexibly switched between the original semi-automatic processing and the original full-automatic processing. The full-automatic machine overcomes the defects of large volume, complex structure, complex operation and high price of the existing full-automatic machine in the market.
According to the technical scheme provided by the invention, the thickness of each piece of tissue cutting is automatically and accurately controlled through the X-axis stepping motor, the Z-axis stepping motor and the linear sliding module, and errors caused by manual operation are eliminated; the direct current servo motor of the Y shaft and the eccentric wheel form a mechanism for converting circular motion into linear reciprocating motion, so that the noise generated by the high-speed motion of the Y shaft and the possibility of increasing the clearance after friction generated by long-term reciprocating motion are effectively reduced; the operation of the whole machine is controlled by adopting the embedded microcontroller, so that the switching of functions such as full-automatic processing, manual processing and the like is realized; the operation of the whole machine is controlled by adopting the embedded microcontroller, so that the switching of functions such as full-automatic processing, manual processing and the like is realized; the thickness of the controllable slice is thinnest to be 1.25um through four subdivision settings of the drivers of the X-axis stepping motor and the Z-axis stepping motor.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a schematic view of an overall structure of a full-automatic vibrating microtome according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a Z-axis assembly according to an embodiment of the present invention;
FIG. 3 is a schematic perspective view of a Z-axis assembly according to an embodiment of the present invention;
FIG. 4 is a schematic perspective view of a Y-axis assembly according to an embodiment of the present invention;
FIG. 5 is a cross-sectional view of an X-axis assembly according to an embodiment of the present invention;
FIG. 6 is a schematic perspective view of an X-axis assembly according to an embodiment of the present invention;
fig. 7 is a schematic perspective view of a sample cartridge according to an embodiment of the present invention;
fig. 8 is a schematic diagram of a control circuit of the full-automatic vibrating microtome according to an embodiment of the present invention.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, if directional indications (such as up, down, left, right, front, back, top and bottom … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.
In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Most of slicing machines on the market at present belong to semi-automatic type, and the thickness of cutting next time that needs artifical manual adjustment is cut one slice at every turn, and both time consuming and also hard like this also can cause because artifical manual adjustment thickness brings various errors simultaneously. In order to solve the technical problem, the invention provides a full-automatic vibrating slicer, which comprises the following steps:
as shown in fig. 1 to 2, a fully automatic vibrating microtome includes a base 10, a sample box 20, a Y-axis assembly 30, and an X-axis assembly 40 and a Z-axis assembly 50 disposed on the base 10; the sample cartridge 20 is disposed on the X-axis assembly 40; the Y-axis assembly 30 is disposed on the Z-axis assembly 50; the Y-axis assembly 30, the sample cartridge 20, and the X-axis assembly 40 are arranged from top to bottom; the Z-axis assembly 50 comprises a Z-axis shell 51, a support 52, a Z-axis stepping motor 53, a Z-axis lead screw 54, a Z-axis guide rail 55, a first connecting plate 56, a second connecting plate 57, a Z-axis sliding block 58 connected with the Z-axis guide rail 55 in a sliding manner, and a limiting bracket 59 arranged on the support 52; the support 52 is arranged on the inner side wall of the Z-axis shell 51, and the Z-axis guide rail 55 is arranged on the side surface of the support 52; the first connecting plate 56 and the second connecting plate 57 are respectively disposed at both ends of the Z-axis guide 55; the Z-axis stepping motor 53 is arranged at one end of the second connecting plate 57 close to the base 10; one end of the Z-axis screw rod 54 is connected to the Z-axis stepping motor 53, and the other end of the Z-axis screw rod 54 passes through the Z-axis slider 58 and is connected to the first connecting plate 56; through Z axle step motor 53 accurate control Z axle slider 58 slides on Z axle guide rail 55, realize slicer automatically regulated cutting thickness, reduce the error, improve the section quality. Compared with the prior art, the semi-automatic slicing machine overcomes the defects that the existing semi-automatic slicing machine consumes time and labor and is inconsistent in cutting precision.
Further, as shown in fig. 2, in the present embodiment, the limit bracket 59 includes a first limit bracket 591 and a second limit bracket 592 arranged opposite to the first limit bracket 591; the Z-axis slider 58 may reciprocate between the first limit bracket 591 and the second limit bracket 592; when the Z-axis slider 58 starts to slide at a predetermined initial position on the side close to the first limit bracket 592 and reaches the position of the second limit bracket 592, the Z-axis slider 58 moves in the reverse direction immediately and automatically returns to the initial position.
Further, as shown in fig. 1 to 2, in this embodiment, one end of the Z-axis slider 58 is connected to the Y-axis assembly 30, and the movement of the Y-axis assembly 30 in the Z-axis direction is realized by the driving of the Z-axis slider 58.
Further, as shown in fig. 3 to 4, in the present embodiment, the Y-axis assembly 30 includes a bracket 301, a Y-axis dc servo motor 302, an eccentric wheel 303, an axis pin 304, a Y-axis guide rail 305, a Y-axis slider 306, a connecting block 307, a knife arm 308, a knife holder 309, and a presser block 310; the Y-axis direct current servo motor 302 is arranged on the side surface of the bracket 301; the Y-axis guide rail 305 is arranged on the side surface of the bracket 301 far away from the Y-axis direct current servo motor 302; one end of the bracket 301, which is close to the Y-axis direct current servo motor 302, is connected with the Z-axis sliding block 58; the Y-axis guide rail 305 is connected with the Y-axis slide block 306 in a sliding manner; one side of the connecting block 307 is connected with the sliding block 306, and the other side of the connecting block 307 is connected with the knife arm 308; one end of the eccentric wheel 303 is connected with the Y-axis dc servo motor 302, and the other end of the eccentric wheel 303 is connected with the connecting block 307 through the shaft pin 304; the blade seat 309 is provided on the cutter arm 308; the blade holder 309 is provided with the blade holder 310; the vibration of the Y-axis component 30 uses the structure of the Y-axis direct current servo motor 302 and the eccentric wheel 303 to convert the circular motion into linear reciprocating motion; the noise generated by the Y-axis assembly 30 during high-speed movement and the possibility of increasing the clearance after friction generated by long-term reciprocating movement are effectively reduced.
One end of the bracket 301, which is close to the Y-axis direct current servo motor 302, passes through a Z-axis through hole 511 formed in the Z-axis housing 51 to be connected with the Z-axis slider 58, so that the danger caused by the movement of the Y-axis assembly 30 can be well prevented.
Further, as shown in fig. 4, in this embodiment, the Y-axis dc servo motor 302 is connected to the eccentric wheel 303, and converts the circular motion of the Y-axis dc servo motor 302 into a linear reciprocating motion to drive the Y-axis slider 306 to reciprocate along the Y-axis guide rail 305; one side of the eccentric wheel 303, which is far away from the Y-axis direct-current servo motor 302, is provided with a first elastic retainer ring 311, and the first elastic retainer ring 311 can effectively ensure the stability of the connection between the Y-axis direct-current servo motor 302 and the eccentric wheel 303.
Further, as shown in fig. 4, in the present embodiment, a washer 312 is disposed between the eccentric wheel 303 and the connecting block 307, so that abrasion of the eccentric wheel and the connecting block due to high pressure generated during high-speed movement can be effectively prevented; besides, a second retaining ring 313 is arranged at one end of the shaft pin 304 close to the connecting block 307, and the second retaining ring 313 can effectively fasten the axial movement of the shaft pin 304, so that the stability of the structure is ensured.
Referring again to fig. 4, in the present embodiment, a first groove 314 is formed on the blade seat 309, and the blade holder 310 is disposed in the first groove 314; when the blade is installed, the blade pressing block 310 presses the blade, the blade is adjusted to a proper position, and the hand-screwed screw is screwed tightly to complete the fixation of the blade; the position and the angle of the blade can be adjusted by loosening the hand-screwed screw according to the needs in the use process.
Further, as shown in fig. 1, 5 and 6, in this embodiment, the X-axis assembly 40 includes an X-axis housing 401, a front cover plate 402, a slot cover plate 403, an X-axis slider 404, an X-axis guide rail 405, a third connecting plate 406, a fourth connecting plate 407, a limiting block 408, an X-axis stepping motor 409, an X-axis lead screw 410 and a sensor 411; the X guide 405 and the sensor 411 are respectively disposed on the base 10; one end of the X-axis guide rail 405 is connected to the third connecting plate 406, and the other end of the X-axis guide rail 405 is connected to the fourth connecting plate 407; the X-axis stepping motor 409 is disposed on one side of the fourth connecting plate 407 away from the X-axis guide rail 405; one end of the X-axis lead screw 410 is connected to the X-axis stepping motor 409, and the other end of the X-axis lead screw 410 passes through the X-axis slider 404 and is connected to the third connecting plate 406; the third connecting plate 406 is arranged on the inner side wall of the X-axis housing 401; the X-axis slide block 404 is connected with the X-axis guide rail 405 in a sliding manner; the slot cover 403 is provided on the X-axis slider 404.
Further, as shown in fig. 6, in this embodiment, the limiting block 408 includes an advancing limiting block 4081 and a retracting limiting block 4082 which are disposed on the same side of the slot cover plate 403; by arranging the advancing limiting block 4081 and the backing limiting block 4082, the advancing and backing of the X-axis assembly are effectively controlled, and slicing is completed.
A movable distance of 4cm is reserved between the fixed screw holes (not marked) arranged on the advancing limiting block 4081 and the backspacing limiting block 4082; before slicing, the advancing limiting block 4081 and the retreating limiting block 4082 are fixed at the middle position of the fixed screw hole (not marked) through a hand-screwed screw; during slicing, moving the forward limiting block 4081 to the side of the backward limiting block 4082 by 2cm, moving the backward limiting block 4082 to the side of the forward limiting block 4081 by 2cm, and fixing by screwing a screw by hand, so that a tissue slice of 4cm can be actually cut to the maximum extent, wherein the distance is far greater than the normal tissue slice area; in the slicing process, the distance between the forward limiting block 4081 and the backward limiting block 4082 can be adjusted according to actual needs, and further the distance of the tissue slice area is adjusted.
Referring again to fig. 1, 5 and 6, in the present embodiment, further, the sensor 411 includes a first sensor 4111 and a second sensor 4112; the distance between the first sensor 4111 and the second sensor 4112 is greater than the distance between the forward limit block 4081 and the backward limit block 4082.
When tissue slicing starts, the X-axis assembly 40 is in an initial state, at this time, the forward limit block 4081 corresponds to the first sensor 4111, the backward limit block 4082 is between the first sensor 4111 and the second sensor 4112, and under the driving of the X-axis stepping motor 409, the X-axis slider 404 starts to move from a side of the first sensor 4111 close to the second sensor 4112 to a side of the second sensor 4112, and at this time, tissue is sliced for the first time; when the retraction limit block 4082 reaches the second sensor 4112, the X slider 404 immediately changes the movement direction to retract toward the first sensor 4111; when the advance limit block 4081 reaches the first sensor 4111, the Z-axis stepping motor 53 moves downward by a set distance according to a set slice thickness, and the X-axis slider 404 changes the movement direction again and moves toward the second sensor 4112 to process a tissue slice, so that the process is repeated until the slicing operation is completed.
In the invention, the X-axis assembly 40 and the Z-axis assembly 50 are all changed into linear sliders controlled by stepping motors for slicing, so that the dovetail groove processing technology of a semi-automatic slicer in the prior art is omitted. The microtome of the present invention is essentially cost-consistent with a semi-automatic microtome.
Further, as shown in fig. 6, in the present embodiment, a sliding groove 4031 is disposed on the slot cover plate 403, and the sliding groove 4031 is slidably connected to the sample box 220 and can be fixed by a hand screw.
Further, as shown in fig. 7, in the present embodiment, the sample cartridge 20 includes a bottom case 21, a circular table 22, and a U-shaped card 23 disposed on the bottom case 21; the processing round table 22 is arranged on the U-shaped clamp 23 through a screw; a boss 211 is arranged at one end of the bottom box 21, and a second groove 212 is arranged at the other end of the bottom box 21; the boss 211 is matched with the sliding groove in a sliding manner; the U-shaped card 23 is disposed in the second recess 212.
Specifically, when slicing, firstly, adding a tissue culture solution into the second groove 212, then fixing the tissue to be sliced on the circular truncated cone 22 by using glue, secondly, adjusting the U-shaped clamp 23 to a proper position, then, screwing down a hand screw, and finally, adjusting the position of the bottom box 21 on the slot cover plate 43, and matching the X-axis assembly 40 and the Y-axis assembly 30 to complete the slicing action.
Fig. 8 shows a schematic diagram of a control circuit of a full-automatic vibrating slicer according to the present invention, in this embodiment, the control system of the full-automatic vibrating slicer includes an embedded microcontroller 1, external key input interface circuits 2 connected to the embedded microcontroller 1, an X-axis and Z-axis driving and direction control circuit 3, a Y-axis vibration control motor driving circuit 4, and an X-axis, Y-axis and Z-axis display driving circuit 5; and an X-axis, Y-axis, Z-axis speed adjusting circuit 6; the slicer of the invention adopts an embedded microcontroller 1 to control the operation of the whole slicer; the Y-axis direct current servo motor 302 utilizes the PWM function of the embedded microcontroller 1, and then the Y-axis vibration control motor driving circuit 4 and the Y-axis speed adjusting circuit function to regulate the speed of the vibration motor; under the regulation of the speed regulating circuits of the X axis and the Z axis, the control motors of the X axis and the Z axis are controlled by adopting stepping motors, and the stepping motors drive the screw rods to rotate; the main control board controls the power amplifier of the stepping motor through the pulse and I/O output of the embedded microcontroller 1, under the action of the X-axis and Z-axis drive and direction control circuit 3, the power amplifier of the stepping motor drives the stepping motor to rotate forwards and backwards to process slices, and the processing speed and thickness can be adjusted through the microcontroller. The screw rod advances by 1mm every time the stepping motor rotates for one circle; each pulse goes 1.8 °; therefore, 200 pulses need to be sent when the X axis and the Z axis advance for 1 mm; the invention divides the drivers of the X-axis stepping motor 409 and the Z-axis stepping motor 53 into four parts, and 800 pulses are required to be sent every 1mm of forward movement. In the technical scheme provided by the invention, the control resolution of the slicer is 1 mm/800-0.00125 mm (namely 1.25um), namely the thickness of the slice which can be controlled by the slicer is 1.25um at the minimum. The processing speed and the cutting thickness can be displayed by driving the LED display screen through the X-axis, Y-axis and Z-axis display driving circuits 5, and the speed and the thickness can be adjusted by the microcontroller through the adjusting buttons of the panel.
The embedded microcontroller technology is adopted for controlling the machine, so that the slicing machine can be flexibly switched between the original semi-automatic processing and the original full-automatic processing. The full-automatic machine overcomes the defects of large volume, complex structure, complex operation and high price of the existing full-automatic machine in the market.
According to the technical scheme provided by the invention, the thickness of each piece of tissue cutting is automatically and accurately controlled through the X-axis stepping motor, the Z-axis stepping motor and the linear sliding module, and errors caused by manual operation are eliminated; the direct current servo motor of the Y shaft and the eccentric wheel form a circular motion to be converted into a linear reciprocating motion mechanism, so that the noise generated by the high-speed motion of the Y shaft and the possibility of increasing the clearance after friction generated by long-term reciprocating motion are effectively reduced; the operation of the whole machine is controlled by adopting the embedded microcontroller, so that the switching of functions such as full-automatic processing, manual processing and the like is realized; the thickness of the controllable slice is thinnest to be 1.25um through four subdivision settings of the drivers of the X-axis stepping motor and the Z-axis stepping motor.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. The full-automatic vibrating slicer is characterized by comprising a base, a sample box, a Y shaft assembly, an X shaft assembly and a Z shaft assembly, wherein the X shaft assembly and the Z shaft assembly are arranged on the base; the sample cartridge is arranged on the X shaft assembly; the Y shaft assembly is arranged on the Z shaft assembly; the Y shaft assembly, the sample box and the X shaft assembly are arranged from top to bottom; the Z-axis assembly comprises a Z-axis shell, a support, a Z-axis stepping motor, a Z-axis screw rod, a Z-axis guide rail, a first connecting plate, a second connecting plate, a Z-axis sliding block connected with the Z-axis guide rail in a sliding manner, and a limiting support arranged on the support; the support is arranged on the inner side wall of the Z-axis shell, and the Z-axis guide rail is arranged on the side surface of the support; the first connecting plate and the second connecting plate are respectively arranged at two ends of the Z-axis guide rail; the Z-axis stepping motor is arranged at one end, close to the base, of the second connecting plate; one end of the Z-axis screw rod is connected to the Z-axis stepping motor, and the other end of the Z-axis screw rod penetrates through the Z-axis sliding block to be connected to the first connecting plate.
2. The full-automatic vibratory microtome according to claim 1, wherein said stop bracket comprises a first stop bracket and a second stop bracket disposed opposite said first stop bracket.
3. The fully automatic vibratory microtome according to claim 1 wherein one end of said Z-axis slide is connected to said Y-axis assembly.
4. The full-automatic vibratory slicer of claim 3 wherein the Y-axis assembly includes a bracket, a DC servo motor, an eccentric, an axle pin, a Y-axis guide, a Y-axis slide, a connecting block, a knife arm, a blade holder and a knife block; the Y-axis direct current servo motor is arranged on the side face of the bracket; the Y-axis guide rail is arranged on the side surface of the bracket far away from the Y-axis direct current servo motor; one end of the bracket, which is close to the Y-axis direct current servo motor, is connected with the Z-axis sliding block; the Y-axis guide rail is connected with the Y-axis sliding block in a sliding manner; one side of the connecting block is connected with the sliding block, and the other side of the connecting block is connected with the cutter arm; one end of the eccentric wheel is connected with the direct current servo motor, and the other end of the eccentric wheel is connected with the connecting block through the shaft pin; the blade seat is arranged on the cutter arm; the knife pressing block is arranged on the blade seat.
5. The full-automatic vibrating microtome according to claim 4, wherein a washer is disposed between the eccentric and the connecting block, and a second retaining ring is disposed on an end of the shaft pin adjacent to the connecting block.
6. The full-automatic vibratory slicer of claim 1 wherein the X-axis assembly comprises an X-axis housing, a front cover plate, a slot cover plate, an X-axis slider, an X-axis guide rail, a third connecting plate, a fourth connecting plate, a stop block, an X-axis stepper motor, an X-axis lead screw and a sensor; the X guide rail and the sensor are respectively arranged on the base; one end of the X-axis guide rail is connected with the third connecting plate, and the other end of the X-axis guide rail is connected with the fourth connecting plate; the X-axis stepping motor is arranged on one side, far away from the X-axis guide rail, of the fourth connecting plate; one end of the X-axis lead screw is connected to the X-axis stepping motor, and the other end of the X-axis lead screw penetrates through the X-axis sliding block and is connected to the third connecting plate; the third connecting plate is arranged on the inner side wall of the X-axis shell; the X-axis sliding block is connected with the X-axis guide rail in a sliding manner; the slot cover plate is arranged on the X-axis sliding block.
7. The full-automatic vibrating microtome according to claim 6, wherein the stop comprises an advancing stop and a retracting stop disposed on the same side of the pocket cover.
8. The fully automatic vibratory microtome according to claim 6 wherein said sensor comprises a first sensor and a second sensor; the distance between the first sensor and the second sensor is larger than the distance between the advancing limiting block and the backing limiting block.
9. The fully automatic vibratory microtome according to claim 1, wherein the sample box comprises a base box, a circular truncated cone, and a U-shaped card disposed on the base box; the processing round table is arranged on the U-shaped clamp through a screw; a boss is arranged at one end of the bottom box, and a second groove is arranged at the other end of the bottom box; the boss is matched with the sliding groove in a sliding manner; the U-shaped card is arranged in the second groove.
10. The full-automatic vibratory microtome according to claim 1, further comprising a control system, said control system including an embedded microcontroller, and external individual key input interface circuits connected to the embedded microcontroller, an X-axis, Z-axis drive and direction control circuit, a Y-axis vibration control motor drive circuit, an X-axis, Y-axis, Z-axis display drive circuit; and X-axis, Y-axis and Z-axis speed adjusting circuits.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011113019.9A CN112197994A (en) | 2020-10-16 | 2020-10-16 | Full-automatic vibrations formula slicer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011113019.9A CN112197994A (en) | 2020-10-16 | 2020-10-16 | Full-automatic vibrations formula slicer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112197994A true CN112197994A (en) | 2021-01-08 |
Family
ID=74009238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011113019.9A Pending CN112197994A (en) | 2020-10-16 | 2020-10-16 | Full-automatic vibrations formula slicer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112197994A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113319899A (en) * | 2021-06-24 | 2021-08-31 | 上海旺泰客科学仪器有限责任公司 | Full-automatic slicer that embedded section processing was used |
| CN114689485A (en) * | 2022-03-02 | 2022-07-01 | 上海旺泰客科学仪器有限责任公司 | Instrument for detecting plant cell turgor pressure and membrane potential |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005020266A1 (en) * | 2005-04-30 | 2006-11-02 | Hess Consult Gmbh | Rotating microtome has eccentric cams allocated to appropriate microtome components at certain angles, and tension bolts or pressure plates arranged between respective eccentric cams and associated microtome components |
| CN101500767A (en) * | 2006-09-06 | 2009-08-05 | 孔健强 | Method and apparatus for cutting fresh tissue slices |
| CN102414549A (en) * | 2009-09-15 | 2012-04-11 | 孔健强 | Slicer |
| CN107116590A (en) * | 2016-02-24 | 2017-09-01 | 百慕立新（天津）生物科技有限公司 | A kind of automatic clinical microtome for biological medicine experimental study |
| CN207027655U (en) * | 2017-08-07 | 2018-02-23 | 浙江省金华市科迪仪器设备有限公司 | Biological tissue section machine |
| CN108318310A (en) * | 2018-05-14 | 2018-07-24 | 上海交通大学医学院附属第九人民医院 | A device for parallel cutting of irregular biological tissue under the guidance of laser scanning |
| CN213516388U (en) * | 2020-10-16 | 2021-06-22 | 上海旺泰客科学仪器有限责任公司 | Full-automatic vibrations formula slicer |
-
2020
- 2020-10-16 CN CN202011113019.9A patent/CN112197994A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005020266A1 (en) * | 2005-04-30 | 2006-11-02 | Hess Consult Gmbh | Rotating microtome has eccentric cams allocated to appropriate microtome components at certain angles, and tension bolts or pressure plates arranged between respective eccentric cams and associated microtome components |
| CN101500767A (en) * | 2006-09-06 | 2009-08-05 | 孔健强 | Method and apparatus for cutting fresh tissue slices |
| CN102414549A (en) * | 2009-09-15 | 2012-04-11 | 孔健强 | Slicer |
| CN107116590A (en) * | 2016-02-24 | 2017-09-01 | 百慕立新（天津）生物科技有限公司 | A kind of automatic clinical microtome for biological medicine experimental study |
| CN207027655U (en) * | 2017-08-07 | 2018-02-23 | 浙江省金华市科迪仪器设备有限公司 | Biological tissue section machine |
| CN108318310A (en) * | 2018-05-14 | 2018-07-24 | 上海交通大学医学院附属第九人民医院 | A device for parallel cutting of irregular biological tissue under the guidance of laser scanning |
| CN213516388U (en) * | 2020-10-16 | 2021-06-22 | 上海旺泰客科学仪器有限责任公司 | Full-automatic vibrations formula slicer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113319899A (en) * | 2021-06-24 | 2021-08-31 | 上海旺泰客科学仪器有限责任公司 | Full-automatic slicer that embedded section processing was used |
| CN114689485A (en) * | 2022-03-02 | 2022-07-01 | 上海旺泰客科学仪器有限责任公司 | Instrument for detecting plant cell turgor pressure and membrane potential |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN213516388U (en) | Full-automatic vibrations formula slicer | |
| CN112197994A (en) | Full-automatic vibrations formula slicer | |
| US8109184B2 (en) | Method and device for cutting fresh tissue slices | |
| JP2014025933A (en) | Microtome having piezoelectric linear actuator | |
| CN211967625U (en) | Battery side cut device | |
| CN208989032U (en) | Fixed costal cartilage cutters and ear and nose surgical instruments | |
| CN211317923U (en) | Pathological microtome | |
| CN218897437U (en) | Probe positioning and mounting device | |
| CN216870350U (en) | An automatic image acquisition device and its X-axis assembly structure | |
| CN211966092U (en) | Cutting device | |
| CN211640003U (en) | Cutter head fine adjustment mechanism of hair scissors | |
| CN208788102U (en) | A kind of stepping feeding mechanism | |
| CN112211857B (en) | Multichannel pipettes | |
| CN212121940U (en) | Cutting processing device of electromechanical main control board | |
| CN208853821U (en) | Cutter Gap adjustment mechanism | |
| JP2589394B2 (en) | Microtome | |
| CN212355950U (en) | Double faced adhesive tape cutting processing equipment | |
| CN220846806U (en) | Template machine integrating mechanical cutters | |
| CN223407035U (en) | A paper cutter cutting blade limiting guide mechanism | |
| CN113319899A (en) | Full-automatic slicer that embedded section processing was used | |
| CN211806355U (en) | High-precision adhesive tape processing device | |
| CN219550076U (en) | Precise lifting device and detection system | |
| CN221232631U (en) | Cutting machine for membrane cloth | |
| CN217802024U (en) | Carbon fiber cutting device | |
| CN214474161U (en) | High-precision closed-loop control slit adjusting device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20250517 Address after: 215300 Jiangsu Province Suzhou City Kunshan City Huajiao Economic Development Zone Jinxing Road 18 Huajiao International Innovation Park Building A12 Room 5-0003 Applicant after: Suzhou Unipuri Precision Instrument Co.,Ltd. Country or region after: China Address before: 202150 building 3 and 4, No.89, sanshahong Road, Chengqiao Town, Chongming District, Shanghai (Shanghai Chongming supply and marketing economic development zone) Applicant before: Shanghai wangtaike Scientific Instrument Co.,Ltd. Country or region before: China |