CN112639480B - Target substance extraction device that allows replacement of magnetic bar blocks - Google Patents
Target substance extraction device that allows replacement of magnetic bar blocks Download PDFInfo
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- CN112639480B CN112639480B CN201980055502.5A CN201980055502A CN112639480B CN 112639480 B CN112639480 B CN 112639480B CN 201980055502 A CN201980055502 A CN 201980055502A CN 112639480 B CN112639480 B CN 112639480B
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- bar magnet
- extraction device
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- 239000013076 target substance Substances 0.000 title claims abstract description 50
- 238000000605 extraction Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 claims description 27
- 102000039446 nucleic acids Human genes 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 6
- 150000007523 nucleic acids Chemical group 0.000 claims description 6
- 238000009434 installation Methods 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- 239000012472 biological sample Substances 0.000 abstract description 8
- 239000006249 magnetic particle Substances 0.000 description 34
- 239000007787 solid Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000005054 agglomeration Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- RQVGAIADHNPSME-UHFFFAOYSA-N azinphos-ethyl Chemical compound C1=CC=C2C(=O)N(CSP(=S)(OCC)OCC)N=NC2=C1 RQVGAIADHNPSME-UHFFFAOYSA-N 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241001631457 Cannula Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 235000012489 doughnuts Nutrition 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0418—Plate elements with several rows of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0418—Plate elements with several rows of samples
- G01N2035/0425—Stacks, magazines or elevators for plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0427—Sample carriers, cuvettes or reaction vessels nestable or stockable
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- Health & Medical Sciences (AREA)
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Abstract
本发明涉及一种能够同时从多个生物样品中提取目标物质的提取装置，更具体来说涉及一种可根据插入套管中的多孔板的种类更换磁棒块的目标物质提取装置。使用时，本发明的目标物质提取装置的操作方式为在具有不同孔数的各种多孔板中，将适合使用目的的多孔板装载在装置内的套管中，且选择并装载配备有适合此多孔板的磁棒的磁棒块。因此，所述装置具有根据待从中提取目标物质的样品的数量或根据待提取目标物质的量对一种设施选择性地应用各种多孔板的优势。
The present invention relates to an extraction device capable of extracting target substances from multiple biological samples at the same time, and more specifically to a target substance extraction device capable of replacing a magnetic bar block according to the type of a porous plate inserted into a sleeve. When in use, the target substance extraction device of the present invention operates by loading a porous plate suitable for the purpose of use into a sleeve in the device among various porous plates with different numbers of holes, and selecting and loading a magnetic bar block equipped with a magnetic bar suitable for this porous plate. Therefore, the device has the advantage of selectively applying various porous plates to a facility according to the number of samples from which the target substance is to be extracted or according to the amount of the target substance to be extracted.
Description
Technical Field
The present invention relates to an extraction device capable of extracting a target substance from a plurality of biological samples at the same time, and more particularly, to a target substance extraction device in which a magnetic stick block can be replaced according to the kind of a porous plate inserted into a cannula.
Background
Various methods for separating nucleic acids, proteins, etc. from biological samples have been developed, and precipitation, liquid phase extraction, electrophoresis, chromatography, etc. are commonly used. However, for easier operation, solid phase extraction has been developed.
The solid phase extraction method is a method using a solid having selectivity or using solid particles manufactured by attaching a ligand having high selectivity to a solid. The solid phase extraction method is based on the principle of first dissolving a biological sample in a solution to which target substances are selectively attached, attaching the target substances to solids, separating the solids from the solution, washing off residual substances on the solids to remove other impurities, and separating the desired target substances from the solids.
In the case of filling a column with solid particles or a column with a filter using a solid-phase extraction method, a solution slowly flows between micropores, and thus a lot of time is required to separate a target substance. To solve this problem, a centrifugal separation method may be used. However, this approach is difficult to automate. In the case of using a pressurizing or vacuum, there is a problem in that the result obtained from the samples is not uniform due to the difference in the flow rate of the solution between the samples when applied to a plurality of samples.
To solve this problem, a technique is employed in which fine magnetic particles having a large surface area are used to attach a target substance in a suspension solution, a magnetic field is applied to coagulate the magnetic particles to which the target substance is attached, and the solution is removed to separate the target substance (US 3,970,518 and US 3,985,649).
The method for separating a target substance using magnetic particles is largely divided into a target substance attaching step, a solution removing and washing step, and a target substance detaching step. The target substance attaching step is a method of uniformly suspending magnetic particles to agglomerate the magnetic particles, wherein the magnetic field is mainly used to agglomerate the magnetic particles. The magnetic field is generated by a permanent magnet or an electromagnet. Generally, unlike electromagnets, permanent magnets are capable of generating a strong magnetic field without thermal radiation. However, the magnetic flux of the permanent magnet cannot be switched, which is also different from the electromagnet. In this regard, the magnetic particle solution must be physically moved between the magnets to control the switching, and thus the permanent magnets are also inconvenient to automate.
The position where the magnetic particles agglomerate is changed by the position where the magnetic field is applied. The location of the magnetic particle agglomeration is critical for efficient removal of the solution and thus location-dependent techniques have been developed. Separation devices using magnetic particles have been developed for wide application in diagnostic test devices and nucleic acid extraction devices that mainly use antigen-antibody reactions. Pasteur Sanofi Diagnostics (U.S. Pat. No. 5,558,839) developed a method of agglomerating magnetic particles at the bottom of 96-well plates and then suspending the magnetic particles. However, this method has a problem in that the bottom magnetic particles are lost when the solution is completely removed. To solve this problem, a method of mounting a magnet, a rotary magnet or a container on the container side surface to suspend magnetic particles and then stopping the rotation of the magnet or container to coagulate the magnetic particles to the wall surface has been developed (WO 96/26011), and Hitachi developed a system capable of coagulation and suspension using a uniform magnetic field and an alternating magnetic field (US 5,770,461). This method is a method of agglomerating and cleaning magnetic particles by attaching the magnetic particles to the surface of a tube wall using a uniform magnetic field, and suspending the magnetic particles using an alternating magnetic field. Amersham International plc a system for shifting a donut magnet in a direction perpendicular to a container to switch magnetic fields has been developed (US 5,897,783). This is a method of annular agglomeration of magnetic particles on the inner wall of a tube. All of these methods are methods of agglomerating magnetic particles in a reaction vessel, removing the solution, pouring a new solution, and resuspending the magnetic particles.
In contrast to these methods, labsystems developed a method of moving magnetic particles in a reaction vessel containing a solution in which the magnetic particles were suspended and moving the magnetic particles again. The system includes a rod capable of moving up and down as a fishing rod and a rod box. A permanent magnet is mounted at the lower end of the rod and the rod box is made of plastic, which transmits magnetic force and seals the rod from contact with the solution (US 6,040,192). The operation is performed by introducing a rod box into a reaction solution in a state that a magnetic rod is pulled out of the rod box, suspending magnetic particles by the rod box moving up and down so as to react the magnetic particles, inserting the magnetic rod fully into the rod box, and agglomerating the magnetic particles on the surface of the magnetic rod box by the magnetic field action of the magnetic rod, thereby attaching a desired target substance to the magnetic particles. The magnetic particles with the target substance attached thereto may then be transferred to the next solution together with the magnetic rod and rod box. After the movement, the magnetic rod is pulled out of the rod box, the magnetic field is removed, and the rod box is moved up and down to suspend the agglomerated magnetic particles in the new solution. BIONEX an automatic nucleic acid extractor having the same operation scheme as above (korean registered patent No. 10-0483684) was developed. This is a method of attaching magnetic particles to a rod box in which various magnetic rods are inserted, and moving the magnetic particles into another solution so as to suspend the magnetic particles in the solution to extract nucleic acids in the same manner as described in the above-mentioned US'192 patent. However, the Labsystems and BIONEX techniques are used to process samples in a row, and therefore there are limitations to processing multiple samples. For this, core Bio System has developed a technology for processing a plurality of samples, such as samples contained in 96-well plates, using magnetic rods arranged in a two-dimensional array manner in an automatic extraction apparatus (korean patent No. 10-0720044).
However, the magnetic rods are made into a plate shape including 96 magnetic rods arranged in a two-dimensional array to accommodate the 96-well plate kit, and thus there is a problem in that the 96-well plate must always be used regardless of the number of biological samples and the amount of target substances to be extracted.
In the present invention, in order to solve the above-described problems, a device configured to selectively use various multi-well plates, i.e., a cannula for dispensing samples, according to the number of biological samples and the amount of target substances to be extracted in the case of extracting the target substances from a plurality of samples at the same time has been developed. For this purpose, a method of changing the bar magnet blocks to adjust the number of bar magnets to be suitable for the number of sleeve holes has been developed, so that it is possible to efficiently extract a target substance using a single apparatus according to the number of biological samples and the amount of the target substance to be extracted.
Disclosure of Invention
[ Problem ]
The object of the present invention is to provide a target substance extraction device configured to select a porous plate suitable for the purpose of use for replacement, and to install a plurality of porous plates having different numbers of holes to extract a target substance.
[ Technical solution ]
In order to achieve the above object, the present invention provides a target substance extraction device comprising a bar magnet block replacement unit (100), including:
A bar magnet block (110) having a bar magnet (111), the bar magnet (111) being configured to be introduced into each well of a multi-well plate to operate;
a bar magnet block holder (120) configured to allow the bar magnet block (110) to be placed thereon, and a bar magnet block holder (120)
A fixing member (130) configured to fix the bar magnet block (110) to the bar magnet block holder (120).
Drawings
Fig. 1 is a perspective view of a target substance extraction device according to an embodiment of the present invention.
Fig. 2 is an explanatory view of an installation method of the magnetic bar block replacement unit 100 according to an embodiment of the present invention.
Fig. 3 is a perspective view of a bar magnet block 110 according to an embodiment of the present invention.
Fig. 4 is a perspective view of a bar magnet cover block 140 according to an embodiment of the present invention.
Fig. 5 is an explanatory view of an installation method of the magnetic bar block exchanging unit 100 adapted to the 96-well plate (a) or the 24-well plate (B) according to another embodiment of the present invention.
Fig. 6 is a perspective view of a sleeve 210 according to an embodiment of the present invention.
Detailed Description
[ Best mode ] for carrying out the invention
The present invention proposes a method for extracting a target substance using a target substance extraction device that does not consider the kind of a multi-well plate to which a biological sample is dispensed or the kind of a cannula to which the multi-well plate is attached. As a means, the present invention proposes a target substance extraction device configured to facilitate selection of a porous plate suitable for the purpose of use for replacement, and to install a plurality of porous plates having different numbers of holes for extraction of a target substance, and to select a bar magnet block having a bar magnet introduced into each hole of the porous plate for operation for replacement and installation accordingly. The object substance extraction device of the present invention is characterized in that a plurality of porous plates and a plurality of bar magnet blocks having bar magnets suitable for the plurality of porous plates are provided together, whereby it is possible to appropriately select and apply one porous plate for one apparatus according to the number of samples to be extracted or the amount of object substance to be extracted.
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Fig. 1 is a perspective view of a target substance extraction device according to an embodiment of the present invention.
According to one embodiment of the present invention, the target substance extracting apparatus includes a bar magnet block replacing unit 100 and a sleeve mounting unit 200.
In the present invention, the bar magnet block replacement unit 100 includes a bar magnet block (110) having a bar magnet (111), the bar magnet (111) being configured to be introduced into each hole of a porous plate to be operated, a bar magnet block holder (120) configured to allow the bar magnet block (110) to be placed thereon, and a fixing member (130) configured to fix the bar magnet block (110) to the bar magnet block holder (120).
In the present invention, as shown in fig. 2, the bar magnet replacing unit 100 may be prepared by fixing the bar magnet 110 to the bar magnet holder 120 using the fixing member 130. However, as another embodiment, the bar magnet 110 may be fixed to the bar magnet holder 120 by a male-female coupling type.
In the present invention, as shown in fig. 3, the bar magnet block 110 is configured in a shape in which a plurality of bar magnets are connected to the lower surface of the plate. The present invention is characterized in that the number of magnetic strips is equal to the number of wells formed in a multi-well plate containing a sample to be extracted using the magnetic stick block 110.
The bar magnet block 110 may exist in various forms having different numbers of bars magnet so as to be exchangeable according to the kind of porous plate inserted in the sleeve installed in the single target substance extracting apparatus installed together. For example, three bar magnet blocks each having 32 bars connected thereto may be connected to each other so as to be suitable for a 96-well plate, two bar magnet blocks each having 32 bars connected thereto may be provided for a 64-well plate, one bar magnet block having the above configuration may be provided for a 32-well plate, three bar magnet blocks each having 8 bars connected thereto may be provided for a 24-well plate, two bar magnet blocks each having 8 bars connected thereto may be provided for a 16-well plate, or bar magnet blocks having 8 bars may be prepared so that the bar magnet blocks may be replaced and used according to the kind of plate used. However, the present invention is not limited to one way of connecting the bar magnets.
Meanwhile, as shown in fig. 4, the bar magnet block replacement unit 100 may further include a bar magnet cover block 140 fastened to the outside of the bar magnet 111 of the bar magnet block 110 to prevent the bar magnet 111 from being contaminated.
In the present invention, the bar magnet covering block 140 being fastened to the outside of the bar magnet 111 of the bar magnet block 110 means that the bar magnet covering block 140 is fixed around the entire or a part of the surface of each bar magnet 111 of the bar magnet block 110 to prevent the bar magnet 111 of the bar magnet block 110 from being contaminated by contacting the sample. In the present invention, the bar magnet cover block 140 is preferably manufactured to be disposable and may be made of various materials suitable for disposal, such as plastic.
In one aspect of the present invention, the present invention is characterized in that the bar magnet cover 140 is fixed to the bar magnet cover holder 150, wherein the bar magnet cover 140 and the bar magnet cover holder 150 are fixed to each other by male-female coupling with each other, or wherein the bar magnet cover 140 is fixed to the bar magnet cover holder 150 using a fixing member 130'. In another aspect of the present invention, the present invention is characterized in that the bar magnet covering block 140 is fixed to the bar magnet block holder 120 so as to be used for fixing the bar magnet block 110, wherein the bar magnet covering block 140 is fixed to the bar magnet block holder 120 by using the fixing member 130' instead of the fixing member 130 so as to be used for fixing the bar magnet block 110 to the bar magnet block holder 120 (fig. 5).
In addition, the present invention is characterized in that the bar magnet blocks 110 and the bar magnet cover blocks 140, which are mounted to the bar magnet block holder 120 or the bar magnet cover block holder 150, are detachable, and are replaced with the bar magnet blocks 110 to which different numbers of bar magnets are connected and the bar magnet cover blocks 140 to which different numbers of bar magnet covers are connected according to purposes.
Meanwhile, the present invention is characterized in that the cannula mounting unit 200 is configured such that a plurality of cannulas (210) each of which is insertable into a porous plate are stacked one on top of another, wherein each cannula (210) is mounted to the cannula transfer tray (220).
The multi-well plate may be a 96-well plate, a 64-well plate, a 32-well plate, a 24-well plate, a 16-well plate, or an 8-well plate, and may be appropriately selected and used according to the number of samples to be extracted or the amount of a target substance to be extracted. In another aspect of the present invention, each of the sleeves 210 may be made in the form of a perforated plate so as to stack the sleeves up and down in a manner mounted on the sleeve transfer tray 220 without inserting a separate perforated plate.
The present invention is characterized in that the bar magnet block 110 and the bar magnet cover block 140 each have the same number of bar magnets as the number of holes of the installed porous plate.
The present invention is characterized in that the fixing means 130 or 130' (means for fixing the bar magnet block 110 or the bar magnet cover block 140 to the bar magnet block holder 120 or the bar magnet cover block holder 150, respectively) is a fastening means such as a permanent magnet, an electromagnet, a vacuum suction means, a clamp, a bolt, a pin, a snap ring, a ball plunger, or a combination thereof. In the case of using the above-described members, the fixing means used in the present invention may be prepared so as to fix the bar magnet 110 or the bar magnet covering block 140 to the bar magnet block holder 120 or the bar magnet covering block holder 150, or to detach the bar magnet 110 or the bar magnet covering block 140 from the bar magnet block holder 120 or the bar magnet covering block holder 150 without using a separate tool or device. The present invention is characterized in that the coupling between the bar magnet 110 or the bar magnet cover 140 and the bar magnet support 120 or the bar magnet cover support 150 is performed using the fixing member 130 or 130' at the correct positions thereof so as to fix the bar magnet 110 or the bar magnet cover 140 to the bar magnet support 120 or the bar magnet cover support 150 at the correct positions thereof, respectively, without any change in the fixing positions thereof.
The invention is characterized in that the target substance is a nucleic acid. However, the present invention is not limited thereto. Any substance (including protein) that can be extracted using magnetic particles and magnetic rods is applicable, without limitation.
Description of the reference numerals
100 Magnetic bar block replacing unit
110 Magnetic bar block
111 Magnetic rod
120 Magnetic bar block support
130,130': Fixed parts
140 Magnetic bar covering block
150 Magnetic bar covering block support
200 Casing pipe mounting unit
210 Casing pipe
211 Perforated plate
220 Sleeve transfer tray
While specific configurations of the invention have been described in detail, those skilled in the art will appreciate that the preferred embodiments are presented in the specification for purposes of illustration and are not to be construed as limiting the scope of the invention. Accordingly, the true scope of the invention should be determined by the following claims and their equivalents.
[ Industrial availability ]
In the case of using the target substance extraction device of the present invention, a porous plate suitable for the purpose of use, particularly various porous plates having different numbers of holes, is installed in the sleeve of the device, and a bar magnet block having a bar magnet adapted thereto is selected and installed, whereby it is possible to selectively use various porous plates for one piece of equipment according to the number of samples of the target substance to be extracted or the amount of the target substance to be extracted.
Claims (6)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020180087415A KR102256776B1 (en) | 2018-07-26 | 2018-07-26 | Target Material Extraction Apparatus with exchangeable Magnetic-Rod-Block |
| KR10-2018-0087415 | 2018-07-26 | ||
| PCT/KR2019/009076 WO2020022742A1 (en) | 2018-07-26 | 2019-07-23 | Target material extraction apparatus with replacement of magnetic bar block allowed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112639480A CN112639480A (en) | 2021-04-09 |
| CN112639480B true CN112639480B (en) | 2025-01-10 |
Family
ID=69180951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980055502.5A Active CN112639480B (en) | 2018-07-26 | 2019-07-23 | Target substance extraction device that allows replacement of magnetic bar blocks |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US12012592B2 (en) |
| EP (1) | EP3828548A4 (en) |
| JP (1) | JP7159442B2 (en) |
| KR (1) | KR102256776B1 (en) |
| CN (1) | CN112639480B (en) |
| WO (1) | WO2020022742A1 (en) |
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| CN111570086B (en) * | 2020-05-20 | 2022-05-17 | 周雨威 | Magnet excellent dividing device |
| CN113943652B (en) * | 2020-07-15 | 2024-03-19 | 致茂电子(苏州)有限公司 | Multi-section magnetic rod module and nucleic acid extraction equipment equipped with the module |
| US12343720B2 (en) * | 2020-11-15 | 2025-07-01 | Life Technologies Corporation | System and method for polynucleotide purification |
| KR102512066B1 (en) * | 2021-04-29 | 2023-03-21 | 주식회사 제놀루션 | Necleic acid extraction device |
| CN113736647A (en) * | 2021-08-20 | 2021-12-03 | 苏州尚元医疗科技有限公司 | Magnetic rod assembly and nucleic acid extraction instrument |
| CN116008531B (en) * | 2021-10-21 | 2025-01-28 | 中检国研(北京)科技有限公司 | A magnetic bead purification instrument |
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Also Published As
| Publication number | Publication date |
|---|---|
| EP3828548A4 (en) | 2022-03-30 |
| KR20200012291A (en) | 2020-02-05 |
| JP2021531164A (en) | 2021-11-18 |
| CN112639480A (en) | 2021-04-09 |
| KR102256776B1 (en) | 2021-05-27 |
| WO2020022742A1 (en) | 2020-01-30 |
| JP7159442B2 (en) | 2022-10-24 |
| EP3828548A1 (en) | 2021-06-02 |
| US12012592B2 (en) | 2024-06-18 |
| US20210230579A1 (en) | 2021-07-29 |
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