CN113180056B - Hypochlorous acid disinfectant - Google Patents

Abstract

A hypochlorous acid disinfectant able to be stored stably contains iodate, and one or more of sulfate, carbonate, dibasic phosphate, monobasic phosphate and bicarbonate.

Description

Hypochlorous acid disinfectant

technical field

The invention relates to a hypochlorous acid disinfectant that can be stably preserved.

Background technique

84 disinfectant is a product that is widely used to kill bacteria and viruses, prevent diseases and inhibit the spread of etc. Sanitary disinfection. 84 disinfectant has ideal disinfection effect, low price, convenient use, broad-spectrum and high-efficiency sterilization characteristics, but it is irritating and corrosive. Gloves are required when using because of irritation to the skin. 84 disinfectant is generally prepared by reacting chlorine (Cl ₂ ) with sodium hydroxide (NaOH),

Cl ₂ +2NaOH=NaClO+H ₂ O+NaCl

The sodium hypochlorite in the reaction product is the main component of the disinfectant. It can be obtained by or by the action of bleaching powder and sodium carbonate, as well as electrolysis of dilute NaCl solution and stirring and mixing the chlorine gas produced by the anode and the NaOH produced by the cathode. 84 disinfectant is a chlorine-containing disinfectant with sodium hypochlorite as the main component. It is a colorless or light yellow liquid with a pungent odor. Generally, the pH is 12-13, and the effective chlorine content is 5.5% to 6.5%.

Sodium hypochlorite is a strong electrolyte. It is first ionized in water to generate hypochlorite ions (ClO-), and hypochlorite ions can be hydrolyzed to generate hypochlorous acid, which is further decomposed to generate new ecological oxygen [O] (a substance directly composed of oxygen atoms) , its chemical properties are very active, and it has strong oxidizing power, which plays a great role in the disinfection process. The disinfection principle of 84 disinfectant is that hypochlorite radicals lyse the bacteria through the redox effect with the bacterial cell wall and virus shell; because of unstable decomposition, new ecological oxygen is generated, and the strong oxidizing property of the new ecological oxygen denatures the proteins of bacteria and viruses. , so that pathogenic microorganisms are lethal; chloride ions can significantly change the osmotic pressure of bacteria and virions, causing them to lose their activity and die; in addition, a small amount of hypochlorous acid produced due to instability also partially kills pathogenic microorganisms.

Now there are technical equipment, such as OSG company AQUACIDO NDX-250KMS, First Ocean (FirstOcean) Co., Ltd. Desktop Fineoxer FO-1000S2, LWas series or Was series-anode-HClO generator or Danish DCW hypochlorous acid generator, by electrolysis To produce hypochlorous acid oxidation potential water to prepare hypochlorous acid disinfectant.

According to the "Sanitary Requirements for Acidic Electrolyzed Water Generators" (GB28234-2020), acidic oxidation potential water, also known as strongly acidic electrolyzed water, is a process of adding softened water to a low-concentration sodium chloride solution and passing through a diaphragm electrolytic cell. The acidic aqueous solution with low concentration of available chlorine and high oxidation-reduction potential produced by electrolysis at the anode has a pH of 2-3 and an oxidation-reduction potential of more than 1100mV. In electrolysis, chlorine, oxygen and H ⁺ are generally generated at the anode, and H ⁺ is dissolved in water to show acidity, and OH ^- is generated at the cathode.

Slightly acidic electrolyzed water, also known as slightly acidic oxidation potential water, is significantly different from 84 disinfectant in preparation, composition and properties, and is also very different from acidic oxidation potential water. Slightly acidic oxidizing potential water is generally produced by adding demineralized water to hydrochloric acid and/or sodium chloride solution and electrolysis in a diaphragm or non-diaphragm electrolytic cell. Among them, chlorine and H ⁺ are generally generated at the anode, and H ⁺ is dissolved in water to show weak acidity. The chlorine reacts with water to generate hydrochloric acid and hypochlorous acid, and the cathode generates hydrogen. The pH of slightly acidic oxidation potential water is less than 7, generally 5-6.5, and its main bactericidal component is hypochlorous acid.

The components contained in the oxidation potential water are relatively complex and have a great influence on the pH value. In simple terms, between pH 4-6, the water-soluble chlorine (Cl ₂ ) is almost zero, and the hypochlorous acid content is the highest; below pH 3, the water-soluble Cl ₂ will increase sharply, while the hypochlorous acid content will sharply increase When the pH is about 8, the content of hypochlorous acid decreases sharply to about 20% and continues to decrease sharply with the increase of pH, while the hypochlorite radical (OCl ^- ) increases sharply. Therefore, the difference in pH results in a large difference in the composition of 84 disinfectant, acidic oxidation potential water, and slightly acidic oxidation potential water.

Both hypochlorite (ClO ^- ) and hypochlorous acid (HCIO) are available chlorine, but their antivirus principles are completely different. The surface of the cell membrane is negatively charged, so hypochlorite (ClO ^- , also negatively charged) cannot easily enter the interior of the cell, while hypochlorous acid (HClO) is a small neutral molecule that can penetrate the cell membrane and enter the interior of the cell, Oxidative reaction with bacterial (virus) body proteins, nucleic acids and enzymes or destroys its phosphate dehydrogenase, resulting in cell death due to imbalance of glucose metabolism, thereby killing pathogenic microorganisms. In biological systems, hypochlorous acid (HClO) is produced by the oxidation of chloride ions under the combined action of a peroxidase derived from neutrophils and hydrogen peroxide. Quantitative analysis showed that about 2×10 ^-7 mol of hypochlorous acid could be produced by 100,000 activated neutrophils within 2 h of incubation. Hypochlorous acid has a good inactivation effect on the virus. Studies have shown that the use of 250mg/L hypochlorous acid on the poliovirus prepared according to the "Technical Specification for Disinfection" for 10min can effectively inactivate the poliovirus. In addition, experiments have shown that hypochlorous acid can effectively kill viruses under the conditions of washing and gasification. In some experiments, human influenza A virus was selected as the test object, and 3.5mg/L hypochlorous acid was used at room temperature for 30s. The test results showed that the logarithm of killing of human influenza A virus was the inactivation effect of the virus (H7N1) of 3.2. The results showed that in the absence of organic matter, hypochlorous acid could reduce the virus titer regardless of the form of direct spray or indirect spray.

According to the existing literature, the bactericidal effect of hypochlorous acid (40ppm) is significantly better than that of sodium hypochlorite (1000ppm) for Gram-positive bacteria and fungi. On the premise of the same initial effective chlorine concentration, even if the dilution ratio is 2-4 times that of strongly acidic electrolyzed water, slightly acidic electrolyzed water has better disinfection and sterilization ability than strongly acidic electrolyzed water.

Hypochlorous acid slightly acidic oxidation potential water has high safety as a disinfectant. But in reality, hypochlorous acid slightly acidic electrolyzed water has complex composition and unstable properties. Although there are some reported methods to enhance stability, such as CN107439588B of Realm Therapeutics Co., Ltd., none of them can meet the requirements of stability experiments, or It is not simple and the cost is high. Considering the long-term demand and the current epidemic situation, there is an urgent need for a hypochlorous acid disinfectant that can be stably stored and can effectively inactivate bacteria and viruses.

SUMMARY OF THE INVENTION

According to one aspect of the present invention, there is provided a hypochlorous acid disinfectant solution that can be stably preserved, which is a slightly acidic oxidation potential water containing halide salt, and also contains disodium phosphate, dihydrogen phosphate, sulfate, carbonic acid One or more of salt and bicarbonate. .

According to yet another aspect of the present invention, there is provided a hypochlorous acid disinfectant solution that can be stored stably, wherein the halide salt is an iodate salt.

According to yet another aspect of the present invention, there is provided a stably preserved hypochlorous acid disinfectant, wherein the iodate is potassium iodate or sodium iodate.

According to yet another aspect of the present invention, there is provided a stably preserved hypochlorous acid disinfectant, wherein the sulfate, carbonate, dihydrogen phosphate, dihydrogen phosphate and bicarbonate are respective sodium or potassium salts .

According to yet another aspect of the present invention, there is provided a stably preserved hypochlorous acid disinfectant, wherein the content of the halide salt is 5-500 ppm.

According to yet another aspect of the present invention, there is provided a hypochlorous acid disinfectant solution that can be stably preserved, wherein the content of the halide salt is 50-200 ppm.

According to another aspect of the present invention, the hypochlorous acid disinfectant that can be stably preserved is provided, wherein the consumption of the disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium bicarbonate, sodium carbonate, and sodium sulfate is respectively 50-400ppm.

According to another aspect of the present invention, a stably preserved hypochlorous acid disinfectant is provided, wherein the available chlorine concentration of the hypochlorous acid disinfectant is 70-600 ppm.

According to another aspect of the present invention, a stably preserved hypochlorous acid disinfectant is provided, wherein the available chlorine concentration of the hypochlorous acid disinfectant is 250-550 ppm.

According to another aspect of the present invention, a stably preserved hypochlorous acid disinfectant is provided, wherein the pH of the slightly acidic oxidation potential water is 4.5-6.8, the oxidation-reduction potential is not higher than 900mV, and the effective chlorine concentration thereof is 70-600ppm between.

According to another aspect of the present invention, a stably preserved hypochlorous acid disinfectant is provided, which is that slightly acidic oxidation potential water contains iodate, as well as disodium hydrogen phosphate, dihydrogen phosphate, carbonate, sulfate One or more of bicarbonate and bicarbonate, and its available chlorine does not drop by more than 5% within 12 months of storage at room temperature.

According to another aspect of the present invention, a kind of method for producing hypochlorous acid disinfectant is provided, and it comprises:

(1) Add iodate to slightly acidic oxidation potential water;

(2) standing for 1 hour to 8 hours; and

(3) adding one or more of sulfate, carbonate, dihydrogen phosphate, dihydrogen phosphate and bicarbonate.

According to another aspect of the present invention, there is provided a method for producing hypochlorous acid disinfectant, wherein the oxidation-reduction potential of the slightly acidic oxidation potential water is not more than 900mV, the pH is between 4.5-6.8, and the available chlorine concentration is between 70-600ppm between.

According to another aspect of the present invention, the method for the production of hypochlorous acid disinfectant is provided, which comprises taking salt as a raw material, controlling the salt consumption of about 5-13 g/g (calculated by available chlorine), and the energy consumption of 5-25W h. /g (calculated as available chlorine) to produce slightly acidic oxidative potential water.

According to another aspect of the present invention, a method for producing hypochlorous acid disinfectant is provided, which includes controlling the output of oxidizing potential water to 500-1500L/hour, the current value is generally between 90-400A, and the salt consumption is about 1-15g/hour. gram (calculated as available chlorine), energy consumption is 1-30W·h/g (calculated as available chlorine) to produce slightly acidic oxidation potential water.

According to another aspect of the present invention, there is provided a hypochlorous acid disinfectant that can be stably preserved, which is that the slightly acidic oxidation potential water contains iodate, disodium hydrogen phosphate and/or dihydrogen phosphate, and carbonate , one or more of sulfate and bicarbonate.

These and other aspects of the invention will be apparent from and elucidated with reference to the embodiments described hereinafter.

Description of drawings

The invention will now be described, by way of example only, in conjunction with the accompanying drawings, in which:

Figure 1 is a schematic diagram of the inventive process flow. The hypochlorous acid solution in the figure is slightly acidic oxidation potential water.

Detailed ways

The hypochlorous acid disinfectant of the present invention is significantly different from the 84 disinfectant in preparation, components and properties, and is also very different from the acid oxidation potential water. The slightly acidic electrolyzed water is used as the raw material of the hypochlorous acid disinfectant of the present invention, and the production process of the present invention is designed as a rapid analysis electrolysis process. The production equipment can be a diaphragm electrolysis unit.

The electrolytic solution is an inorganic salt solution, a slightly acidic solution or a mixture of the two. For example, in an electrolytic cell with a diaphragm, generally sodium chloride, the content is about 1-15%, with concentrated brine, such as saturated salt water, mixed with softened water and pumped, the general consumption of sodium chloride is 1-20g /L (anode electrolyzed water). The anode of the electrolytic cell mainly undergoes chlorine evolution reaction, with very little oxygen evolution, producing slightly acidic oxidation potential water with hypochlorous acid as the main component; the cathode mainly undergoes hydrogen evolution reaction to generate alkaline reduction potential water, with a pH of about 12-13, and the cathode The sodium hydroxide produced is generally below 0.5%, preferably below 0.1%, more preferably below 0.08%. In addition, the slightly acidic oxidation potential water produced by the anode has a relatively low sodium ion content (preferably less than 0.9%), and its main active chlorine component is hypochlorous acid; generally it contains about 70-650 ppm of available chlorine, preferably 100-610 ppm of available chlorine Chlorine, more preferably 105-600 ppm, more preferably slightly acidic electrolyzed water containing 110-570 ppm of available chlorine, wherein the main component of available chlorine is hypochlorous acid. The redox potential of the effluent from the anode is not more than 1000mV, preferably not more than 900mV, preferably 600-900mV, more preferably 700-850mV; its pH is controlled to be 4-7, preferably 4.5-6.8, more preferably 5-6.5.

There are already technical equipment, such as OSG's AQUACIDO NDX-250KMS, Daiichi Ocean's Fineoxer FO-1000S2, LWas series or Was series-anode-HClO generator, Danish DCW hypochlorous acid generator, etc. Production of hypochlorous acid oxidation potential water.

It can control the oxidation potential water output 500-1500L/hour, the current value is generally between 90-400A, preferably not less than 100A, the salt consumption is about 2-15 g/g available chlorine, preferably 5-13 g/g available chlorine , the energy consumption is 1-30W·hr/g available chlorine, preferably the energy consumption is 5-25W·hr/g available chlorine, and about 50-80% of the chloride ions are converted.

The produced slightly acidic electrolyzed water can be left for a period of time, such as 0.5, 1, 2, 3, 6, 8, 10, 12, 20, 24, 36, 48, 60, 72 hours, preferably 0.5-24 hours, more preferably 1-8 hours.

Stabilizers are generally organic or inorganic salts such as formate or acetate or phosphate or silicate or halide or hypochlorite or chlorite or perchlorate One or more mixtures of alkali metal or alkaline earth metal water-soluble salts of bromate, carbonate or bicarbonate, acetate or sulfamate or citrate, preferably Inorganic salts, especially their potassium or sodium salts, more preferably halides, especially iodates, and one of sulfates, carbonates, hydrogen phosphates, dihydrogen phosphates and hydrogen carbonates A mixture of one or more of them, especially one or more of their potassium and sodium salts.

Halogenates refer to oxo acid salts whose valence of halogen elements is +5, such as chlorates, bromates, iodates, preferably their sodium or potassium salts, more preferably iodates, such as NaIO ₃ , KIO ₃ etc.

The total added amount of the stabilizer is generally not more than 0.5% by weight, preferably not more than 0.1%. The amount of each component (including halogen salts, organic salts and inorganic salts) added is generally 0.0005%-0.05% (ie 5-500ppm), preferably 0.005%-0.04% (ie 50-400ppm), more preferably 0.015% %-0.03%, such as 100, 120, 150, 170, 200, 220, 250, 270, 300, 350, 400, 500ppm.

The order of adding the stabilizer is generally to add a halide salt, such as an iodate, to the slightly acidic electrolyzed water produced, and then add other stabilizers after standing for a period of time.

Generally the final product contains about 70-600ppm of available chlorine, preferably 100-580ppm, more preferably 160-570ppm, more preferably slightly acidic electrolyzed water containing 250-550ppm of available chlorine, wherein the main component of available chlorine is hypochlorous acid, generally greater than 80%, preferably greater than 90%, such as 90-92%, more preferably greater than 95%.

Softened water can generally be tap water after softening treatment or purified water produced by pure water production equipment, and its total hardness is less than 25mg/L (calculated as CaCO ₃ ).

Fig. 1 is the process flow schematic diagram of the present invention. The raw water is purified by activated carbon adsorption filtration, ultrafiltration and reverse osmosis filtration. Purified water is pumped into the electrolyzer under the control of a constant pressure valve. At the same time, high-concentration chloride salt solution (concentration>15%), such as saturated salt water, is pumped into the water inlet pipe before the electrolyzer through a special pump. The pumping amount of chloride salt and the current applied to the electrolytic cell vary according to the available chlorine concentration of the hypochlorous acid solution at the outlet of the electrolytic cell. The production process of the invention is a rapid analysis electrolysis process, the current is controlled at 100-350A (according to different product specifications), and the redox potential is controlled below 900mV. The amount of softened water is controlled at 900-1800L/hour, and the chloride content in the electrolytic cell is estimated to be about 1-15%, preferably 2-10%. Purified water is pumped into the electrolyzer under the control of a constant pressure valve. Slightly acidic oxidation potential water is the effluent of the anode, and its amount is about 60-70% of the total effluent; the cathode mainly produces sodium hydroxide solution, and its output is about 30-40% of the total effluent.

The slightly acidic oxidizing potential water hypochlorous acid disinfectant of the present invention has good disinfection effect and high stability through the control of raw materials and production process flow. According to the accelerated stability test of "Technical Specifications for Disinfection" (Ministry of Health, 2002 edition), after 14 days of storage at 54°C, and the rate of decline of sterilization active ingredients is less than or equal to 10%, the storage validity period can be set as 1 year. It is determined that the effective chlorine of the disinfectant of the present invention is reduced by no more than 5%, which is far better than the 10% requirement in the "Disinfection Technical Specification". Therefore, the hypochlorous acid disinfectant of the slightly acidic oxidation potential water of the present invention can be stably stored for a long time, for example, at least 12 months, 18 months, or 24 months. Especially for the high-concentration hypochlorous acid electrolyzed water disinfectant that is more difficult to store for a long time, especially the hypochlorous acid electrolyzed water disinfectant with an available chlorine content of 250ppm or more, such as 250-550ppm hypochlorous acid electrolyzed water disinfectant, can achieve the above stable preservation effect.

Example 1

Saturated brine is pumped from the batching tank into the purified water inflow pipeline before the electrolytic cell, the purified water pressure is controlled at 1-1.5bar, the current is 106-120A, the anodic oxidation potential water outlet flow is maintained at 1200L/hour, and the pH is controlled at 5.5-6.7. The effective chlorine concentration is between 95 and 125ppm, and the redox potential is 800-900mV. To the obtained electrolyzed hypochlorous acid water, 50 ppm of potassium iodate was added, and it was allowed to stand for 8 hours, and then 200 ppm of sodium dihydrogen phosphate, sodium sulfate and sodium bicarbonate were added.

Detect pH and effective chlorine concentration. The effective chlorine test is in accordance with the "Technical Specification for Disinfection" (Ministry of Health, 2002 edition), and the effective chlorine content is 105ppm.

The pH meter YQC-034 is tested in accordance with the "Technical Specification for Disinfection" (Ministry of Health, 2002 edition), and the ambient temperature is 22 °C and the relative humidity is 47%. The pH (25 °C) two serial numbers are 6.31 and 6.31, and the average value is 6.31 .

Example 2

Saturated brine is pumped from the batching tank into the purified water inflow pipeline before the electrolytic cell, the purified water pressure is controlled at 1-2bar, the current is 160-220A, the anodic oxidation potential water outlet flow is maintained at 600-700L/hour, pH5.5-6.7, The effective chlorine concentration is between 250 and 280 ppm, and the redox potential is 750-900 mV. The obtained hypochlorous acid electrolyzed water was added with 160 ppm potassium iodate, left standing for 10 hours, and then 200 ppm each of sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium sulfate and sodium carbonate were added.

Detect pH and effective chlorine concentration. The effective chlorine detection is in accordance with the "Technical Specification for Disinfection" (Ministry of Health, 2002 edition), and the effective chlorine content is 262ppm.

The pH meter YQC-034 is tested in accordance with the "Technical Specification for Disinfection" (Ministry of Health, 2002 edition), and the ambient temperature is 22°C and the relative humidity is 47%. The pH (25°C) two serial numbers are 6.29 and 6.29, and the average is 6.29.

Example 3

Saturated salt water is pumped from the batching tank into the purified water inflow pipe before the electrolytic cell, the water pressure is controlled at 1.5-2bar, the current is 230A-276A, the effluent flow of the anode potential water is 1200L/hour, the pH is 4.5-6.0, and the effective chlorine concentration is 540～ Between 610ppm, the redox potential is 700-850mV, the salt consumption is about 5-7 grams per liter of anode electrolyzed water, and the energy consumption is about 12W·hr (watt-hour). The obtained hypochlorous acid electrolyzed water was added with 200 ppm of potassium iodate, allowed to stand for 8 hours, and then 150 ppm of sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium sulfate, sodium carbonate and sodium bicarbonate were added.

Detect pH and effective chlorine concentration. The effective chlorine detection is in accordance with the "Disinfection Technical Specification" (Ministry of Health, 2002 edition), and the effective chlorine content is 598ppm.

The pH meter YQC-034 is tested in accordance with the "Technical Specification for Disinfection" (Ministry of Health, 2002 edition), and the ambient temperature is 20°C and the relative humidity is 48%. The pH (25°C) two serial numbers are 4.75 and 4.75, and the average is 4.75.

Example 4

The hypochlorous acid disinfectant obtained in Example 1 was tested for stability. The inspection is based on the "Technical Specification for Disinfection" (2002 edition of the Ministry of Health), and the storage conditions are: placed in a 54 ℃ incubator for 14 days. Detection environment temperature: 22 ℃, relative humidity 47%.

Example 5

The hypochlorous acid disinfectant obtained in Example 2 was tested for stability. The inspection is based on the "Technical Specification for Disinfection" (2002 edition of the Ministry of Health), and the storage conditions are: placed in a 54 ℃ incubator for 14 days. Detection environment temperature: 22 ℃, relative humidity 47%.

Example 6

The hypochlorous acid disinfectant obtained in Example 3 was tested for stability. The inspection is based on the "Technical Specification for Disinfection" (2002 edition of the Ministry of Health), and the storage conditions are: placed in a 54 ℃ incubator for 14 days. Detection environment temperature: 20 ℃, relative humidity 48%.

Example 7

The hypochlorous acid disinfectant that embodiment 1 obtains carries out bacterial quantitative killing experiment

Test strain name:

Escherichia coli, strain number: 8099, strain generation: fifth generation;

Staphylococcus aureus, strain number: ATCC6538, strain generation: fifth generation;

Pseudomonas aeruginosa, strain number: ATCC 15442, strain generation: the third generation;

Candida albicans, strain number: ATCC10231, strain generation: fifth generation

Strain source: ATCC

Equipment : biological safety cabinet YQF-057, biochemical incubator YQF-058, biochemical incubator YQF-159.

Inspection basis :

1. Technical Specifications for Disinfection, Ministry of Health, 2002;

2. Quantitative Determination Test of Neutralizer Suspension According to Technical Specifications for Disinfection, Ministry of Health, 2002; and

3. Detection ambient temperature: 23°C, relative humidity 45%.

Test conclusion:

1. The neutralizer meets the test requirements.

2. The action time is 0.5 minutes, 1 minute and 1.5 minutes, and the test is repeated 3 times. The logarithmic killing values of Escherichia coli (8099), Common Coccus aureus (ATCC6538), and Pseudomonas aeruginosa (ATCC15442) are all >5.00 , the tested strains are qualified for disinfection.

3. The action time is 0.5 minutes, the test is repeated three times, the logarithm of killing against Candida albicans is less than 4.00, and the average logarithmic value is 3.81; The strains tested were qualified for disinfection.

Example 8

The hypochlorous acid disinfectant that embodiment 1 obtains carries out virus inactivation experiment

Virus name and host:

Poliovirus-I vaccine strain, Vero cells

Equipment : carbon dioxide incubator (QFM-B-B004), low temperature thermostat DKB-1915 (QFM-B-P014), biological safety cabinet (QFM-B-B031), inverted microscope (QFM-B-B012).

Inspection basis :

1. Technical Specifications for Disinfection, Ministry of Health, 2002;

2. Detection of ambient temperature: 23.2 ℃, relative humidity 51%.

Testing method

The action time is 0.5 minutes, divided into 5 groups, and the identification test of the physical removal method of residual disinfectant is carried out;

The action time is 0.5 minutes, 1 minute, 1.5 minutes, repeated 3 times, and the test temperature is 20 °C ± 1 °C.

Test results:

1. The test results show that the measured physical drug removal method is qualified.

2. In the 0.5-minute test, the average logarithm of inactivation was 3.07, and the virus inactivation rate was 99.91%;

In the 1-minute test, the average log inactivation value was 4.08, and the virus inactivation rate was 99.99%;

In the 1.5-minute test, the average log inactivation value was >4.10, and the virus inactivation rate was >99.99%.

Example 9

The hypochlorous acid disinfectant that embodiment 1 obtains carries out hard surface disinfection test

equipment

Neutralizer: 0.1% sodium thiosulfate PBS;

Diluent: 0.1% Tween 80 in PBS (0.03mol/L) solution

sterile cotton swab

Test sample: wooden table top

method

Test basis: "Disinfection Technical Specification" 2002 edition

Disinfection method: take an appropriate amount of sample, spray it on the wooden surface, and disinfect for 15 minutes

Detection environment: temperature 21 ℃, humidity 46%

The experiment was repeated 3 times, with 30 samples per test.

Test results : The average killing control value of natural bacteria on the wood surface for three times is: >1.73, >1.84, >1.77, so the average is >1.78, which is qualified for disinfection.

Example 10

The hypochlorous acid disinfectant that embodiment 2 obtains carries out bacterial quantitative killing experiment

Test strain name:

Escherichia coli, strain number: 8099, strain generation: sixth generation;

Staphylococcus aureus, strain number: ATCC6538, strain generation: sixth generation;

Pseudomonas aeruginosa, strain number: ATCC 15442, strain generation: the third generation;

Candida albicans, strain number: ATCC10231, strain generation: sixth generation

Strain source: ATCC

Equipment : biological safety cabinet YQF-057, biochemical incubator YQF-159.

Inspection basis :

1. Technical Specifications for Disinfection, Ministry of Health, 2002;

2. Quantitative determination test of neutralizer suspension; and

3. Detection of ambient temperature: 23 ℃, relative humidity 46%.

Test conclusion:

1. The neutralizer meets the test requirements.

2. The concentration of the sample is 50%, the action time is 0.5 minutes, 1 minute, 1.5 minutes, the test is repeated 3 times, the killing of Escherichia coli (8099), Common Coccus aureus (ATCC6538), Pseudomonas aeruginosa (ATCC15442) The logarithmic values of sterilization are all >5.00, and the tested strains are qualified for sterilization.

3. The concentration of the sample is 50%, the action time is 0.5 minutes, the test is repeated three times, the logarithm of killing Candida albicans is less than 4.00, the average logarithmic value is 3.87, and the average bactericidal rate is 99.98%; the action time is 1 minute, 1.5 minutes, the test is repeated 3 times, the logarithm of killing is >4.00, and the tested strains are qualified for disinfection.

Example 11

The hypochlorous acid disinfectant that embodiment 2 obtains carries out virus inactivation experiment

Virus name and host:

Poliovirus-I vaccine strain, Vero cells

Equipment : carbon dioxide incubator (QFM-B-B004), low temperature thermostat DKB-1915 (QFM-B-P014), biological safety cabinet (QFM-B-B031), inverted microscope (QFM-B-B012).

Inspection basis :

1. Technical Specifications for Disinfection, Ministry of Health, 2002;

2. Detection of ambient temperature: 23.2 ℃, relative humidity 51%.

Testing method

The sample action concentration is 1:1, the action time is 0.5 minutes, and it is divided into 5 groups to carry out the identification test of the physical removal method of the residual disinfectant;

The concentration of the sample is 1:1, the action time is 0.5 minutes, 1 minute, and 1.5 minutes, repeated 3 times, and the test temperature is 20 °C ± 1 °C.

Test results:

1. The physical drug removal method measured on the surface of the test results is qualified.

2. In the 0.5-minute test, the average logarithm of inactivation was 2.04, and the virus inactivation rate was 99.06%;

In the 1-minute test, the average log inactivation value was >4.37, and the virus inactivation rate was >99.99%;

In the 1.5-minute test, the average log inactivation value was >4.21, and the virus inactivation rate was >99.99%.

Example 12

The hypochlorous acid disinfectant that embodiment 3 obtains carries out bacterial quantitative killing experiment

Test strain name:

Escherichia coli, strain number: 8099, strain generation: fourth generation;

Staphylococcus aureus, strain number: ATCC6538, strain generation: fourth generation;

Pseudomonas aeruginosa, strain number: ATCC 15442, strain generation: fourth generation;

Candida albicans, strain number: ATCC10231, strain generation: fourth generation

Bacillus subtilis var. black spore, strain number: ATCC9372, strain generation: fifth generation

Strain source: ATCC

Equipment : biological safety cabinet YQF-057, biochemical incubator YQF-159.

Inspection method :

1. Technical Specifications for Disinfection, Ministry of Health, 2002;

2. Quantitative Determination Test of Neutralizer Suspension According to Technical Specifications for Disinfection, Ministry of Health, 2002; and

3. Detection ambient temperature: 20°C, relative humidity 42%.

4. Sample preparation: Measure 200ml of the sample, dilute to 1000ml with hard water, and set aside.

Test conclusion:

1. The neutralizer meets the test requirements.

2. The concentration of the sample is 20%, the action time is 0.5 minutes, 1 minute and 1.5 minutes, and the test is repeated 3 times. The logarithmic values of sterilization are all >5.00, and the tested strains are qualified for sterilization.

3. The concentration of the sample is 20%, the action time is 0.5 minutes, 1 minute and 1.5 minutes. The test is repeated three times. The logarithm of killing Candida albicans is >4.00, and the sterilization rate is >99.99%. The tested strains are qualified for disinfection.

4. The original concentration of the sample, the action time of 20 minutes, 40 minutes and 60 minutes, the test was repeated three times, the logarithm of killing of Bacillus subtilis var. black spores was >5.00, and the average sterilization rate was >99.999%. The tested strains are qualified for disinfection. .

Example 13

The hypochlorous acid disinfectant that embodiment 3 obtains carries out virus inactivation experiment

Virus name and host:

Poliovirus-I vaccine strain, Vero cells

Equipment : 37°C carbon dioxide incubator (QFM-B-B045), cryostat DKB-1915 (QFM-B-P014), biological safety cabinet (QFM-B-B031), inverted microscope (QFM-B-B012) .

Inspection basis :

1. Technical Specifications for Disinfection, Ministry of Health, 2002;

2. Detection of ambient temperature: 20.5 ℃, relative humidity 52%.

Testing method

The sample concentration of 20%, the action time of 1 minute, divided into 5 groups, the test temperature is 20 ℃ ± 1 ℃, and the identification test of the physical removal method of residual disinfectant is carried out;

The concentration of the sample was 20%, the action time was 1 minute, repeated 3 times, and the test temperature was 20 °C ± 1 °C.

Test results:

1. The test results show that the measured physical drug removal method is qualified.

In the 2.1-minute test, the average log inactivation value was 4.01, and the virus inactivation rate was 99.99%.

In addition to the above tests, other microbial index test, lead, arsenic, mercury index determination test, metal corrosion test, drinking water disinfection field test, hard surface disinfection test, air disinfection simulation field test, hand disinfection test, forearm skin test Disinfection test, artificial bacteria stained porcelain plate disinfection test, cucumber surface sterilization test are all qualified; inhalation toxicity test, damaged skin irritation test, acute oral toxicity test, acute eye irritation test, vaginal mucosa irritation test, mouse bone marrow erythrocyte micronucleus test, etc. , the results all prove that the product of the present invention is non-toxic, non-irritating, and has no damage to chromosomes in vivo.

In the present invention, "plurality" refers to at least two.

While the claims have been set forth in this application as specific combinations of features, and some embodiments have been shown and described, those skilled in the art will appreciate that these embodiments may be modified without departing from the principles of the invention Changes are made, and the scope of the invention is defined in the claims. Those skilled in the art will appreciate that modifications (additions and/or deletions) to the components, ingredients, systems described herein may be made without departing from the full scope and spirit of the invention, which encompasses such modifications and any and all equivalents thereof.

Claims (9)

1. A hypochlorous acid disinfectant which can be stably stored is subacid oxidation potential water and contains 500ppm of iodate, and also contains one or more of dibasic phosphate, monobasic phosphate, sulfate, carbonate and bicarbonate with the dosage of 50-400ppm respectively, wherein the effective chlorine concentration is 250-600 ppm.

2. The hypochlorous acid disinfectant of claim 1, wherein the iodate is potassium iodate or sodium iodate.

3. The hypochlorous acid disinfectant of claim 1, wherein the sulfate, carbonate, dibasic phosphate, monobasic phosphate, and bicarbonate salts are each sodium or potassium salts.

4. The hypochlorous acid disinfectant of claim 1, wherein the effective chlorine concentration of the hypochlorous acid disinfectant is 250-550 ppm.

5. The hypochlorous acid disinfectant of claim 1, wherein the slightly acidic electrolyzed oxidizing water has a pH of 4.5 to 6.8 and an oxidation-reduction potential of not more than 900 mV.

6. The hypochlorous acid disinfectant of claim 1, which is slightly acidic electrolyzed oxidizing water containing iodate and one or more of dibasic phosphate, monobasic phosphate, carbonate, sulfate, and bicarbonate, and which has an effective chlorine decrease of no more than 5% within 12 months of storage at ambient temperature.

7. A method of producing the hypochlorous acid disinfectant of claim 1, comprising:

(1) adding iodate into subacid oxidation potential water;

(2) standing for 1-8 hours; and

(3) adding one or more of sulfate, carbonate, dibasic phosphate, monobasic phosphate and bicarbonate.

8. The method of claim 7 wherein the slightly acidic oxidative potential water has an oxidation-reduction potential of no more than 900mV and a pH of between 4.5 and 6.8.

9. The method of claim 7, wherein common salt is used as a raw material in the production of the slightly acidic electrolyzed oxidizing water, and common salt consumption is controlled to be 5 to 13 g/g of available chlorine, and energy consumption is controlled to be 5 to 25W-h/g of available chlorine.

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