CN113396922B - Composition of pigment synthesis inhibitor and safener for controlling gramineous weeds in winter wheat field and application thereof - Google Patents

Abstract

The invention discloses two kinds of pigment synthesis inhibitors (difenpyr and isoxaflutole) and three kinds of safeners (difenoxen, mefenoxen and mefenpyrid) in combination respectively, and bisazole The oxalicil-pyrafenpyr (trifenpyr, isoxadifen) composition at a dose of 210+6.57～105g a.i./ha can significantly alleviate the phytotoxicity of the pesticide on Zhoumai 27 seedlings, isoxafen The ketone-bisoxadifen (hexafen, mefenpyr) composition at a dose of 300+9.38～75ga.i./ha can significantly alleviate the phytotoxicity of the medicament on Zhoumai 27 seedlings without affecting The resistance to the virulence of Fenoxaprop-Pen and Mesosulfuron-resistant Aurantia can solve the dilemma that no stem and leaf treatment agents are available for drug-resistant grass weeds in wheat fields. It is a feasible synergistic herbicide safener combination for the control and control of fenoxaprop-prop and mesosulfuron-methyl fenugreek in winter wheat fields.

Description

A pigment synthesis inhibitor and safener combination for controlling gramineous weeds in winter wheat field compound and its application

technical field

The invention belongs to the technical field of plant protection, and relates to the synergistic application of two pigment synthesis inhibitors and three safeners respectively to prevent and control drug-resistant gramineous weeds in winter wheat fields.

Background technique

Wheat is one of the food crops with the widest distribution range, the largest planting area and the highest yield in the world. The safe production of wheat is crucial to the development of the world economy and even human beings. Among the food crops headed by wheat, rice and corn, the The proportion of production has been rising. my country is the world's largest wheat producer, consumer and importer. According to statistics, the total sown area of wheat in my country in 2020 will be 22,711 thousand hectares (340.66 million mu), a decrease of 273.5 thousand hectares (4.102 million mu) compared with 2019, a decrease of 1.2%. It was 23.9875 million hectares, and the total wheat output was 131.68 million tons (263.4 billion catties), an increase of 756,000 tons (1.51 billion catties) over 2019, an increase of 0.6%.

Cucumber is an annual or transannual grass herb, mostly born in waterside and humid places, and widely distributed all over the world; it is also widely distributed in waterside wetlands in Northeast my country, North China, Northwest China, East China, Southwest China and other provinces; my country In the rice stubble rapeseed fields and wheat fields in the middle and lower reaches of the Yangtze River, the occurrence of weeds showed an increasing trend, and gradually increased from the original secondary weeds to the main weeds in wheat fields, which brought new hazards to wheat production. Kanmai Niang is mainly distributed in China, Turkey, Japan and other Asian countries, which can easily cause a large reduction in crop production. It is widely distributed in the eastern, central and southwestern parts of my country and parts of the Yellow River Basin, especially in rice fields, wheat fields and rape fields south of the Huaihe River Basin in the Qinling Mountains. Chemical weeding plays an important role in weed control because of its time-saving, labor-saving, high-efficiency and quick-acting effects on weeds, and is currently the most important way to control weeds in wheat fields. ACCase (acetyl coenzyme A carboxylase) inhibitors and ALS (acetolactate synthase) inhibitor herbicides are the most commonly used stem and leaf treatment agents for controlling annual grass weeds in wheat fields. and Kanmai Niang have been reported to be resistant to these two types of agents, among which the anti-fine oxaprop-op-p-p-p-p-p. Drugs such as pyridinium and pinoxaden have produced different degrees of cross-resistance and multi-resistance, and Metsulfuron-methyl-resistant Amyniang is also resistant to other ALS inhibitors such as nicosulfuron-methyl, metsulfuron-methyl, and sulfone Cross-resistance occurred among rimsulfuron-methyl, fluasulfuron-methyl, acesulfame and penoxsulam. Therefore, the prevention and control of Cucumis chinensis and Alopecia areata is faced with the dilemma that there is no herbicide available for stem and leaf treatment.

Pigment synthesis inhibitors target the enzymes in the biosynthesis of photosynthetic pigments, and are an important direction and hotspot in the development of herbicides. Among them, bisacenil and isoxaflutole have attracted more attention. Bisacenil, a protoporphyrinogen oxidase (PPO) inhibitor, is a contact herbicide that exerts its medicinal effect through the accumulation of porphyrinogen oxidase in plant nerves. The weeds of the family Scyperaceae Yinglin, cattle felt, broad-leaved weeds Psyllium yujiuhua, Moshangcai, and perennial wild arrowhead mushrooms and water chestnuts all have high activity. Isoxaflutole is an organic heterocyclic selective systemic pre-emergence herbicide, which inhibits weed photosynthesis by inhibiting weed p-hydroxypyruvate dioxygenase (HPPD), hindering chlorophyll biosynthesis, and can control many Grass weeds (such as crabgrass, goosegrass, green foxtail, barnyardgrass, and daughter of a daughter, etc.) At present, these two drugs are registered for use in rice fields and corn fields in China, but have not yet been registered for use in wheat fields. The mechanism of action of bisacenil and isoxaflutole is different from that of ACCase and ALS inhibitors, and they have the potential to control weeds resistant to ACCase and ALS inhibitor herbicides, but their safety to wheat is unknown. and ALS inhibitor activity in grass weeds is unknown.

Herbicide safeners are a class of chemical substances that can be used simultaneously with herbicides to protect crops from herbicide damage. The present invention studies the safety of different safeners and pigment synthesis inhibitors to wheat, and finds that the combination of clofenac, isoxadifen, mefenpyrid and two kinds of pigment synthesis inhibitors has a higher effect on wheat. It has good safety, and it has good indoor activity against Fenoxaprop-Pen and Mesosulfuron-resistant Amygonia at the same time, and has the potential to control resistant weeds in wheat fields.

Contents of the invention

The object of the present invention is to provide a combination of a pigment synthesis inhibitor and a safener that can be safely used in wheat fields to control grass weeds resistant to ACCase and ALS inhibitors in wheat fields.

The beneficial effect of the present invention is that after combining the pigment synthesis inhibitor which is unsafe for wheat growth and the safener, it is safe for wheat growth, and at the same time, it has a good indoor inhibitory effect on grass weeds resistant to ACCase and ALS inhibitors in wheat fields, and has Potential for controlling resistant grass weeds in wheat fields.

The technical scheme adopted in the present invention is:

Combination of herbicides and safeners, the main components of which are the PPO inhibitor fenfenpyr (HPPD inhibitor isoxaflutole) and one of the safeners chlorpyrifos, isoxadifen, and pymeclofen-ethyl. To control resistant grass weeds in winter wheat fields.

Combination of pigment synthesis inhibitors and safeners:

After wheat sowing and before emergence, when weeds are not unearthed, the soil is sealed.

detailed description

(1) Safety of mixing pigment synthesis inhibitors and safeners on wheat - laboratory test

The indoor experiment was conducted from April to May 2020 in the solar greenhouse of the Herbicide Toxicology and Resistance Laboratory of Nanjing Agricultural University.

Herbicides tested: 20% dipacenil SC (Jiangsu Zhongqi), 75% isoxaflutole WDG (Hangzhou Yingtai Biotechnology Co., Ltd.)

Safeners for testing: mefenpyr-methyl (Shanghai Yuanye Biological Co., Ltd.), isoxadifen (Shanghai Beide Pharmaceutical Technology Co., Ltd.), fenpyrid (Hangzhou Qingfeng Agrochemical Co., Ltd.)

Wheat for testing: Zhoumai 27, purchased in Henan in October 2019; Anke 1401, purchased in Anhui Province in October 2019

Indoor experiment process

1. Test agent and safener dose setting

Dosage settings of dipacenil and safener are as follows:

Difenpyr dose: 0, 210g a.i./ha.

Safener dosage: 0, 6.57, 13.13, 26.25, 52.5, 105, 210g a.i./ha.

Dosage settings for isoxaflutole and safener are as follows:

Dose of isoxaflutole: 0, 300g a.i./ha.

Safener dosage: 0, 9.38, 18.75, 37.5, 75, 150, 300g a.i./ha.

Pigment synthesis inhibitors and safeners are mixed in pairs.

2. Test method

Prepare nutrient soil according to field soil: nutrient matrix 2: 1. The pH of nutrient soil is 6.1, and the organic matter content is 1.4%. Put nutrient soil into a small plastic box with a size of 7 × 7 × 7 cm and holes at the bottom, add water to the bottom to make the soil Absorb water to saturation. Choose plump wheat seeds and sow them according to the standard of 15 grains per small plastic box. Grown in a solar greenhouse. The greenhouse temperature was set at 20°C for light and 15°C for darkness, with a light cycle of 12:12h (D:L). The spray adopts the 3WP-2000 walking biometric spray tower produced by the Nanjing Agricultural Mechanization Research Institute of the Ministry of Agriculture. Liquid volume 20mL. After treatment, they were grown in a solar greenhouse. 30 days after the treatment, cut off the aerial part of each treated wheat, weigh the fresh weight, and calculate the fresh weight inhibition rate. Processed with DPS software, carried out herbicide to the fresh weight inhibition rate of wheat and Duncan's new multiple range method to carry out significant analysis (p=0.05)

Inhibition rate % of fresh weight = (fresh weight of control - fresh weight of treatment) / fresh weight of control × 100

It can be seen from Table 1 that the inhibitory rate on the fresh weight of wheat seedlings by dipacenil and isoxaflutole decreased after adding different safeners, but with the increase of safener dosage, the inhibitory rate on the fresh weight of wheat seedlings decreased. The effect also increases. At the dose of 210g a.i./ha, the inhibitory rate of bifenpyr on the fresh weight of Zhoumai 27 seedlings can reach 60.97%. It can significantly reduce the inhibitory rate of dipacenil to the fresh weight of Zhoumai 27 seedlings. When the dipacenil-pyrexamin composition is at the dose of 210+6.57g a.i./ha, its effect on the fresh weight of Zhoumai 27 seedlings The inhibition rate was only 0.30%. When used in combination with isoxadifen, isoxadifen could significantly reduce the inhibitory rate of dipenclonil on the fresh weight of Zhoumai 27 seedlings at a dose of 6.57g a.i./ha. At the dose of 210+6.57ga.i./ha of the mefenpyr-difenoxen-acid composition, its inhibition rate on the fresh weight of Zhoumai 27 seedlings was -3.64%; In the range of .i./ha dose, it can significantly reduce the inhibitory rate of pyrenil to the fresh weight of Zhoumai 27 seedlings. When it was lowered, its inhibitory rate to the fresh weight of Zhoumai 27 seedlings was -6.06%. In contrast, mefenpyr-ethyl had the best detoxification effect on dipacenil. Based on these results, it is recommended to use 6.57-105g a.i./ha of fenpyrid or mefenpyr-ethyl (6.57g a.i./ha of isoxadifen) mixed with 210g a.i./ha of fenfenpyr for soil Closed treatment is relatively safe for the growth of Zhoumai 27 seedlings.

At a dose of 300g a.i./ha, isoxaflutole can inhibit the fresh weight of Anke 1401 seedlings by 29.07%. Both can significantly reduce the fresh weight inhibition rate of isoxaflutole on Anke 1401 seedlings. The fresh weight inhibition rate of isoxaflutole was 7.44%. When used in combination with isoxadifen, isoxadifen could significantly reduce the inhibitory effect of isoxaflutole on Anke 1401 seedlings within the dose range of 6.57-300g a.i./ha. Fresh weight inhibition rate, when the isoxaflutole-isoxadifen composition is at a dose of 300+9.38g a.i./ha, its fresh weight inhibition rate to Anke 1401 seedlings is -5.71%; In the dose range of 9.38～300g a.i./ha, the inhibitory rate of isoxaflutole on the fresh weight of Anke 1401 seedlings could be significantly reduced. At the dose of ga.i./ha, the fresh weight inhibition rate of Anke 1401 seedlings was -1.90%. In contrast, isoxadifen had the best detoxification effect on isoxaflutole. Therefore, it is recommended to use 9.38g a.i./ha of fenpyrid or mefenpyr-ethyl (9.38-75g a.i./ha isoxadifen) mixed with 300g a.i./ha of isoxaflutole for soil sealing It is relatively safe for the growth of Anke 1401 seedlings.

Table 1 Effects of mixed use of pigment synthesis inhibitors and different safeners on the fresh weight of wheat seedlings

*: Lowercase letters are significant differences at the 0.05 level using the new multiple range method of DPS software Duncan

(2) Experiment of the combination of pigment synthesis inhibitor and safener against the virulence of the drug-resistant Smilax chinensis and Alopecia radiata-indoor test:

The indoor experiment was conducted from April to May 2020 in the solar greenhouse of the Herbicide Toxicology and Resistance Laboratory of Nanjing Agricultural University.

Herbicides to be tested: 20% dipacenil SC (produced by Jiangsu Zhongqi), 75% isoxaflutole WDG (Hangzhou Yingtai Biotechnology Co., Ltd.)

Safeners for testing: mefenpyr-methyl (Shanghai Yuanye Biological Co., Ltd.), isoxadifen (Shanghai Beide Pharmaceutical Technology Co., Ltd.), fenpyrid (Hangzhou Qingfeng Agrochemical Co., Ltd.)

The weeds tested: the resistant fenugreek population JYGC, the anti-fenoxaprop-ethyl, the ED ₅₀ value is 1334g ai/ha, the relative resistance multiple is 30.32, collected from the wheat field of Jiangdu District, Yangzhou City, Jiangsu Province in May 2012 ;Resistance depends on the Mai Niang population JTJY-1, which is resistant to methylsulfuron-methyl, with an ED ₅₀ value of 10.45 and a relative resistance multiple of 5.4. It was collected from a wheat field in Jiangyan District, Taizhou City, Jiangsu Province in 2012.

Indoor experiment process

1. Test agent and safener dose setting

Dosage settings of dipacenil and safener are as follows:

Difenpyr dose: 0, 210g a.i./ha.

Safener dosage: 0, 6.57, 13.13, 26.25, 52.5, 105, 210g a.i./ha.

Dosage settings for isoxaflutole and safener are as follows:

Dose of isoxaflutole: 0, 300g a.i./ha.

Safener dosage: 0, 9.38, 18.75, 37.5, 75, 150, 300g a.i./ha.

Pigment synthesis inhibitors and safeners are mixed in pairs.

2. Test method

Prepare nutrient soil according to field soil: nutrient matrix 2: 1. The pH of nutrient soil is 6.1, and the organic matter content is 1.4%. Put nutrient soil into a small plastic box with a size of 7 × 7 × 7 cm and holes at the bottom, add water to the bottom to make the soil Absorb water to saturation. Select the seeds of drug-resistant scorpion grass and kannada that have been released from dormancy, and sow them according to the standard of 30 seeds in each small plastic box. After sowing, cover with a layer of fine soil, wait until the bottom absorbs water to saturation, and spray before the seedlings after sowing. After the medicinal solution is absorbed, it is then placed in a solar greenhouse for growth. The greenhouse temperature was set at 20°C for light and 15°C for darkness, with a light cycle of 12:12h (D:L). The spray adopts the 3WP-2000 walking biometric spray tower produced by the Nanjing Agricultural Mechanization Research Institute of the Ministry of Agriculture. Liquid volume 20mL. After treatment, continue to grow in the solar greenhouse. 30 days after application, the aboveground parts of the treated weeds were cut off, the fresh weight was weighed, and the fresh weight inhibition rate was calculated. Process with DPS software, carry out the significant analysis (p=0.05) of herbicide to the fresh weight inhibition rate of weeds and Duncan's new multiple range method

Inhibition rate % of fresh weight = (fresh weight of control - fresh weight of treatment) / fresh weight of control × 100

It can be seen from Table 2 that the addition of different doses of safener will not reduce the inhibitory effect of dipacenil and isoxaflutole on resistant grass weeds in wheat fields. At the dose of 210g a.i./ha, fenfenpyr, the inhibition rate of the fresh weight of the fenoxaprop-prop-sweet population JYGC was 92.84%. Combination of various doses, the fresh weight inhibition rate of the anti-pyrafenazole population JYGC reached 100%, which was significantly higher than that of fenfenpyr without safener. Considering the above results and cost considerations, it is recommended to use 6.57g a.i./ha of fenpyridine, isoxadifen, and mefenpyr-ethyl in combination with 210g a.i./ha of fenfenpyr for soil sealing treatment. The inhibitory rate against fenoxaprop-albumin JYGC can reach 100%, and it is relatively safe for the growth of Zhoumai 27 seedlings. Isoxaflutole at a dose of 300g a.i./ha inhibited the fresh weight of the mesosulfuron-methyl Amiria population JTJY-1 by 92.12%. The combined use of different doses of clofadrox-methyl, the inhibition rate of the fresh weight of anti-methylsulfuron-methyl Amiria population JTJY-1 was above 90%, and there was no significant difference with isoxaflutole without safener. Considering the above results and cost considerations, it is recommended to use 9.38g a.i./ha of isoxadifen mixed with 300g a.i./ha of isoxaflutole to resist the fresh weight of mesosulfuron kanmai Niang JTJY-1 The inhibition rate is above 90%, and it is relatively safe for the growth of Anke 1401 seedlings.

Table 2 Effects of pigment synthesis inhibitors mixed with different safeners on the fresh weight of resistant weeds

*: Lowercase letters are significant differences at the 0.05 level using the new multiple range method of DPS software Duncan

(3) Herbicidal effect test of mixed use of pigment synthesis inhibitor and safener on weeds in wheat field - indoor test:

The indoor experiment was conducted from April to May 2020 in the solar greenhouse of the Herbicide Toxicology and Resistance Laboratory of Nanjing Agricultural University.

Herbicides tested: 20% dipacenil SC (Jiangsu Zhongqi), 75% isoxaflutole WDG (Hangzhou Yingtai Biotechnology Co., Ltd.)

Safeners for testing: pyraclofen (Shanghai Yuanye Biological Company), isoxadifen (Shanghai Beide Pharmaceutical Technology Co., Ltd.)

The weeds tested: resistant Japanese Kanmai Niang population JCJT-2: resistant to fenoxaprop-ethyl, ED ₅₀ value is 524.23g ai/ha, relative resistance multiple is 34.56, collected from Changzhou, Jiangsu Province in May 2012 Wheat field in Jintan City.

The resistant fenugreek population JYGC: fenoxaprop-ethyl, ED ₅₀ was 1334g ai/ha, and the relative resistance multiple was 30.32. It was collected from the wheat field in Jiangdu District, Yangzhou City, Jiangsu Province in May 2012.

The resistance of Mai Niang population JTJY-1: resistant to methylsulfuron-methyl, ED ₅₀ value of 10.45g ai/ha, relative resistance multiple of 5.4, collected in May 2012 from the wheat field of Jiangyan City, Taizhou, Jiangsu Province.

Sensitive Japanese wheatgrass JNXW-2 group: collected from the wheat field of Nanjing Academy of Agricultural Sciences, Jiangsu Province in May 2011.

JNJP, a sensitive population of Lemongrass: collected from a wheat field in Nanjing City, Jiangsu Province in May 2015.

Sensitive Kan Mai Niang population JNXW-1: Collected in May 2012 from a wheat field in Jiangyan City, Taizhou, Jiangsu Province.

Sensitive multiflora ryegrass population JLGY-45: collected from the wheat field of Huanghai Farm Demonstration Garden in Lianyungang City, Jiangsu Province in May 2019.

Sensitive brome population JLGY-51: collected from the wheat field of Xidan Village, Yinzhuang, Lianyungang City, Jiangsu Province in July 2019.

Sensitive wheat population SDDZ-2.: Collected from the wheat field in Dezhou, Shandong in June 2019.

Sensitive clubhead grass population SLDK-2: collected from the wheat field base of Dongku Village, Lutang Town, Shanghai in June 2019.

Sensitive swine fever population YSXQ: collected from the wheat field of Xiaqiao Village, Yingshang County, Fuyang City, Anhui Province in 2019.

Sensitive vetch population JCWJ-8: collected from the wheat field of Nongba Village, Qianhuang Town, Wujin District, Changzhou City, Jiangsu Province in 2019.

Sensitive shepherd's purse population JJXN-28: collected from the wheat field of Xinmin Village, Xiannv Town, Jiangdu District, Jiangsu Province in May 2019.

Sensitive Popona population JYFN-3: Collected from the wheat field of Funing County, Yancheng City, Jiangsu Province in May 2019.

Sensitive wild geranium population JYFN-2: Collected from a wheat field in Funing County, Yancheng City, Jiangsu Province in May 2019.

Indoor experiment process

1. Test agent and safener dose setting

Dosage settings of dipacenil and safener are as follows:

Difenpyr dose: 0, 210g a.i./ha.

Dosage of mefenpyr-ethyl: 0, 6.57, 13.13, 26.25, 52.5, 105, 210g a.i./ha.

Dosage settings for isoxaflutole and safener are as follows:

Dose of isoxaflutole: 0, 300g a.i./ha.

Isoxadifen dosage: 0, 9.38, 18.75, 37.5, 75, 150, 300g a.i./ha.

Pigment synthesis inhibitors and safeners are mixed in pairs.

2. Toxicity test process of combination of pigment synthesis inhibitor and safener on common weeds in wheat field

Prepare nutrient soil according to field soil: nutrient matrix 2: 1. The pH of nutrient soil is 6.1, and the organic matter content is 1.4%. Put nutrient soil into a small plastic box with a size of 7 × 7 × 7 cm and holes at the bottom, add water to the bottom to make the soil Absorb water to saturation. . Select the weed seeds in the wheat field that have been released from dormancy, sow according to the standard of 30 grains per small plastic box, cover with a layer of fine soil after sowing, wait for the bottom to absorb water to saturation, spray before the seedlings after sowing, and wait for the liquid to absorb Then, they were grown in a solar greenhouse. The greenhouse temperature was set at 20°C for light and 15°C for darkness, with a light cycle of 12:12h (D:L). The spray adopts the 3WP-2000 walking biometric spray tower produced by the Nanjing Agricultural Mechanization Research Institute of the Ministry of Agriculture. Liquid volume 20mL. After treatment, continue to grow in the solar greenhouse. 30 days after application, the aboveground parts of the treated weeds were cut off, the fresh weight was weighed, and the fresh weight inhibition rate was calculated. Processed with DPS software, carried out herbicide to the fresh weight inhibition rate of weeds and Duncan's new multiple range method to carry out significant analysis (p=0.05)

Inhibition rate % of fresh weight = (fresh weight of control - fresh weight of treatment) / fresh weight of control × 100

It can be seen from Table 3 that there is no significant difference in the fresh weight inhibition rate of the weed populations in the 15 wheat fields compared with the combination of fenfenpyr-pyrafenpyr and the single agent of fenfenpyr. Combination of mefenpyr-pyrafen-pyr-methyl can resist the inhibition of fresh weight of fenoxaprop-ethyl in Japanese Kanmai Niang population JCJT-2 under the premise of ensuring relatively safe growth of Zhoumai 27 seedlings at the dose of 210+6.57g a.i./ha The rate was 93.38%. The fresh weight inhibition rate of JYGC against mesosulfuron-methyl kangrain JTJY-1 and oxaprop-alin populations could reach 100%. The fresh weight inhibition rate of the Nest vegetable population JCWJ-8 was above 90%, and the fresh weight inhibition rate of the sensitive population JLGY-45 of ryegrass multiflora, the population SDDZ-2 of S. Look at the freshness of the sensitive population JNXW-1 of Mai Niang, the sensitive population JNJP of Osmanthus chinensis, the YSXQ population of pig scorpion, the JYFN-3 population of Popona, the JYFN-2 population of wild geranium, the JJXN-28 population of shepherd's purse, and the SLDK-2 population of C. Reinhibition rate can reach 100%

It can be seen from Table 4 that the combination of isoxaflutole-bisoxazone and isoxaflutole alone has no significant difference in the fresh weight inhibition rate of common weed populations in 15 wheat fields, indicating that the addition of isoxaflutole is safe. There is no significant effect on the drug after the dose. Isoxaflutole-Bisoxadifen Combination Anti-Fresh Weight Inhibition of Fenoxaprop-Prim in Japanese Kanmai Niang Population JCJT-2 under the Precondition of Ensuring the Growth Safety of Anke 1401 Seedlings at 300+9.38g a.i./ha The rate was 92.23%, the inhibitory rate for the fresh weight of JYGC population against fenoxaprop-ethyl was 93.83%, the inhibitory rate for the fresh weight of JTJY-1 population against mesosulfuron-methyl was up to 100%, and the inhibitory rate for the fresh weight of A. The fresh weight inhibition rate of the sensitive population JNXW-1, the Popona population JYFN-3, the shepherd's purse population JJXN-28 and the clubhead grass population SLDK-2 can reach 100%. The fresh weight inhibition rates of the sensitive population JNJP, the scorpion population YSXQ, the vetch population JCWJ-8, the ryegrass multiflora population JLGY-45, and the barley wheat population SDDZ-2 were all above 80%. -51 and wild geranium population JYFN-2 had a fresh weight inhibition rate below 80%.

Claims (6)

1. A herbicide composition for controlling weeds in winter wheat fields, characterized in that: the composition comprises a herbicidal active ingredient and a safener, wherein the herbicidal active ingredient is dipacenil, and the safener is isoxadifen The weight ratio of the herbicidal active ingredient to the safener is 1:1-32:1, and the herbicide composition is used for controlling weeds in winter wheat fields. 2 . The herbicide composition according to claim 1 , characterized in that: the application rate of dipacenil and isoxadifen is 210+6.57˜105 g a.i./ha. 3. The herbicide composition according to claim 1, characterized in that the weight ratio of isoxadifen to dipenpyril is 1-16:32. 4. The herbicide composition according to claim 1 is used for preventing and controlling resistant grass weeds in winter wheat fields. 5. The use according to claim 4, characterized in that: the method of using the composition is to carry out soil sealing treatment on the weeds and wheat by spraying before the emergence of wheat weeds. 6 . The use according to claim 4 , characterized in that the application rate of dipacenil and isoxadifen is 210+6.57-105 g a.i./ha.