CN113933089B - A device for extracting trace tissue from phloem of citrus huanglongbing tree - Google Patents
A device for extracting trace tissue from phloem of citrus huanglongbing tree Download PDFInfo
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- CN113933089B CN113933089B CN202111206321.3A CN202111206321A CN113933089B CN 113933089 B CN113933089 B CN 113933089B CN 202111206321 A CN202111206321 A CN 202111206321A CN 113933089 B CN113933089 B CN 113933089B
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- 241000207199 Citrus Species 0.000 title description 9
- 235000020971 citrus fruits Nutrition 0.000 title description 8
- 241001478315 Candidatus Liberibacter asiaticus Species 0.000 title description 6
- 238000005070 sampling Methods 0.000 claims abstract description 124
- 238000004321 preservation Methods 0.000 claims abstract description 4
- 238000007599 discharging Methods 0.000 claims abstract 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 3
- 210000001503 joint Anatomy 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 3
- LLLILZLFKGJCCV-UHFFFAOYSA-M n-methyl-n-[(1-methylpyridin-1-ium-4-yl)methylideneamino]aniline;methyl sulfate Chemical compound COS([O-])(=O)=O.C=1C=CC=CC=1N(C)\N=C\C1=CC=[N+](C)C=C1 LLLILZLFKGJCCV-UHFFFAOYSA-M 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 7
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000002826 coolant Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001478324 Liberibacter Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001674048 Phthiraptera Species 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- Sampling And Sample Adjustment (AREA)
Abstract
Description
技术领域technical field
本发明涉及生物技术领域，具体为一种生物技术领域的植物韧皮部微量组织提取装置。The invention relates to the field of biotechnology, in particular to a plant phloem microtissue extraction device in the field of biotechnology.
背景技术Background technique
柑橘黄龙病是柑橘产业的一种毁灭性病害，广泛分布于亚洲、非洲、美洲等柑橘主产区，由一种迄今尚不能人工培养的韧皮部限制性细菌（Candiatus Liberibacter）引起，田间通过柑橘木虱为唯一自然传播媒介进行传播。目前生产上无抗病品种可用，树体一旦染病无法治愈，只能砍除，因其给柑橘产业带来的毁灭性危害被喻为柑橘产业的“癌症”。Citrus huanglongbing is a devastating disease of the citrus industry. It is widely distributed in major citrus producing areas such as Asia, Africa, and the Americas. It is caused by a phloem-restricted bacterium (Candiatus Liberibacter) Lice are the only natural vector for transmission. At present, there are no disease-resistant varieties available in production. Once the tree is infected, it cannot be cured and can only be cut down. Because of the devastating damage it brings to the citrus industry, it is called the "cancer" of the citrus industry.
为加强生产中柑橘黄龙病的监测防控，常需对果园柑橘植株大量采集枝条后送至实验室进行DNA的抽取与黄龙病检测。目前人工采集的枝条偏大，易对树体造成伤害，且不方便快捷大量采样，同时采集的枝条在存运过程中易受保存温度或其他因素污染等影响，导致采集的枝条萎焉、腐烂损坏，难以保证后续黄龙病检测的准确性，因此有必要研发一种柑橘黄龙病树体韧皮部微量组织提取装置，实现连续对树体进行微量取样和采集样本的实时低温保存。In order to strengthen the monitoring and control of citrus Huanglongbing disease in production, it is often necessary to collect a large number of branches of citrus plants in the orchard and send them to the laboratory for DNA extraction and Huanglongbing detection. At present, the branches collected manually are too large, which is easy to cause damage to the tree body, and it is inconvenient to sample a large number of quickly and quickly. At the same time, the collected branches are easily affected by the storage temperature or other factors during storage and transportation, resulting in wilting and rot of the collected branches. Therefore, it is necessary to develop a device for extracting trace tissue from the phloem of citrus Huanglongbing trees, so as to realize continuous micro-sampling of trees and real-time low-temperature storage of collected samples.
发明内容Contents of the invention
本发明的目的在于提供一种树体韧皮部微量取样装置，具备连续取样能力并实时低温保存，且样本不交叉，可用于黄龙病树体韧皮部微量取样等领域。The purpose of the present invention is to provide a micro-sampling device for the phloem of trees, capable of continuous sampling and real-time low-temperature storage, and the samples do not cross, which can be used in the field of micro-sampling of phloem of Huanglongbing trees.
为达到上述目的，本发明所采用的技术方案为：本发明所涉及微量取样装置，包括框体、存储区和操作区，其特征在于：所述框体为长方体结构，所述存储区设置在所述框体的底部，所述操作区固定在所述框体上部空间；所述存储区采用抽屉式结构推拉设计，所述存储区前面板上设置有拉手；所述操作区分割为多组固定的操作隔间，每个所述操作隔间由左右两侧的两块挡板和所述框体前后两条边闭合形成操作空间，每个操作隔间可以单独实施操作，所述挡板嵌入并固定连接到所述框体的前后两个面上，所述操作隔间远离所述拉手一侧嵌入可更换取样管，所述操作隔间底部固定安装有底板用来支撑所述取样管以及用来隔离操作区和存储区，所述操作隔间两侧的挡板侧面安装一段导轨，所述取样管安装到所述导轨上，所述取样管可以在所述导轨上前后自由滑动，所述底板靠近所述拉手一侧设置有下样孔，所述下样孔正下方为所述存储区，所述下样孔设置有盖板，所述盖板安装在所述底板下面，所述盖板可以通过嵌入凹槽等方式嵌入到所述操作区的所述挡板上，所述盖板可在所述底板下面前后拨动，所述盖板拨动到所述下样孔下方时正好将所述下样孔封闭，要保存取样时用手或螺丝刀等工具拨动所述盖板将下样孔露出，所述取样管滑动到所述下样孔正上方时脱离导轨可以进入到所述存储区。In order to achieve the above object, the technical solution adopted in the present invention is: the micro-sampling device involved in the present invention includes a frame body, a storage area and an operation area, and is characterized in that: the frame body is a cuboid structure, and the storage area is arranged on The bottom of the frame body, the operation area is fixed in the upper space of the frame body; the storage area adopts a drawer structure push-pull design, and a handle is arranged on the front panel of the storage area; the operation area is divided into multiple groups Fixed operating compartments, each operating compartment is closed by two baffles on the left and right sides and the front and rear sides of the frame to form an operating space, each operating compartment can be operated independently, the baffles Embedded and fixedly connected to the front and rear surfaces of the frame body, the side of the operating compartment away from the handle is embedded with a replaceable sampling tube, and the bottom of the operating compartment is fixedly installed with a bottom plate to support the sampling tube And to isolate the operation area and the storage area, a section of guide rail is installed on the side of the baffle plate on both sides of the operation compartment, the sampling tube is installed on the guide rail, and the sampling tube can slide freely back and forth on the guide rail, A sample lowering hole is provided on the side of the bottom plate close to the handle, the storage area is directly below the sample lowering hole, and a cover plate is arranged on the sample lowering hole, and the cover plate is installed under the bottom plate. The cover plate can be embedded into the baffle plate of the operation area by embedding grooves, etc., the cover plate can be moved back and forth under the bottom plate, and the cover plate can be moved to the bottom of the sample hole When the sampling hole is to be closed, use a hand or a screwdriver or other tools to move the cover to expose the sample hole when sampling. When the sampling tube slides directly above the sample hole, it can be separated from the guide rail and can enter to the storage area.
树体韧皮部微量取样装置，其特征在于：所述存储区设计为推拉式抽屉安装在所述框体的底部，便于批量取出样本，所述抽屉分为前后两部分，前端设置有多组存储隔间，一组所述存储隔间对应正上方的一组所述操作隔间，能分别将不同操作隔间获得的样本存放在对应的存储隔间内，防止样本交叉接触，便于取样人员能连续取样，且能保存不同来源的样本；所述抽屉后部为冷却区，所述冷却区装入冰块或液氮即可实现对样本进行低温保存，可用于迅速冷冻生物组织，防止生物组织被破坏。The tree phloem micro-sampling device is characterized in that: the storage area is designed as a push-pull drawer installed at the bottom of the frame, which is convenient for taking out samples in batches. One set of storage compartments corresponds to a set of operation compartments directly above, and samples obtained from different operation compartments can be stored in corresponding storage compartments to prevent cross-contact of samples, so that sampling personnel can continuously Sampling, and can save samples from different sources; the back of the drawer is a cooling area, and the cooling area can be filled with ice or liquid nitrogen to realize low-temperature preservation of samples, which can be used to rapidly freeze biological tissues to prevent biological tissues from being destroy.
所述取样管一侧顶部设置螺纹孔，所述取样管另一侧设置为取样针头形状，所述取样管由操作杆进行控制取样或进入存储区，所述操作杆底部设置有外螺纹，所述操作杆通过所述外螺纹对接拧入所述螺纹孔而连接到所述取样管上，从而通过所述操作杆可以推拉所述取样管在导轨上前后移动，所述操作杆推拉到所述下样孔上方时连同所述取样管一起落入到对应的所述存储隔间，旋开所述外螺纹取出所述操作杆，则所述取样管保存到了所述存储隔间，拨动所述盖板到所述下样孔下方即可将所述存储隔间封闭起来。A threaded hole is set on the top of one side of the sampling tube, and the other side of the sampling tube is set in the shape of a sampling needle. The sampling tube is controlled by an operating rod to take samples or enter the storage area. The bottom of the operating rod is provided with external threads, so The operating rod is screwed into the threaded hole through the external thread and connected to the sampling tube, so that the sampling tube can be pushed and pulled by the operating rod to move back and forth on the guide rail, and the operating rod is pushed and pulled to the When above the sample hole, it falls into the corresponding storage compartment together with the sampling tube, unscrew the external thread to take out the operating rod, then the sampling tube is stored in the storage compartment, and turn the The storage compartment can be closed by placing the cover plate under the sample injection hole.
所述取样管的取样针头可以是锥形刀口、斜切刀口或弧切刀口等形状，便于针对不同硬度和厚度的树体选择合适的取样针头，所述取样针头上面设置刻度线，可实现对目标树体取样深度的定量。The sampling needle of the sampling tube can be in the shape of a tapered knife-edge, an oblique knife-edge or an arc-cut knife-edge, which is convenient for selecting a suitable sampling needle for trees with different hardness and thickness. Quantification of target tree sampling depth.
取样前，在所述抽屉的冷却区中装入冰块或液氮等冷却剂，将所述抽屉装入所述框体底部的存储区中，拨动所述盖板将所述下样孔封闭以免污染所述存储区，将所述取样管装入到所述操作隔间中，将所述操作杆通过所述外螺纹连接到所述取样管上。Before sampling, put ice cubes or liquid nitrogen and other coolants in the cooling area of the drawer, put the drawer into the storage area at the bottom of the frame, and turn the cover plate to close the sample hole. Closed so as not to contaminate the storage area, the sampling tube is loaded into the operating compartment, the operating rod is connected to the sampling tube via the external thread.
取样时，推拉所述操作杆使所述取样管沿所述导轨往前移动至所述取样针头露出所述框体，继续推动所述操作杆即可将所述取样针头扎入树体韧皮部进行取样，取样完成后往后拉所述操作杆使所述取样管离开所述树体。When sampling, push and pull the operating rod to move the sampling tube forward along the guide rail until the sampling needle exposes the frame, and continue to push the operating rod to pierce the sampling needle into the phloem of the tree body Sampling, after the sampling is completed, pull the operating rod back to make the sampling tube leave the tree body.
保持样本时，如图所示先拨动所述盖板，确保将所述下样孔露出来，将所述操作杆往后拉至所述下样孔上方，旋开所述外螺纹将所述操作杆取出，则携带样本的取样管落入对应的所述存储隔间进行保存，拨动所述盖板将所述下样孔盖上以封闭对应的所述存储隔间。在下一个操作隔间重复上述操作即可完成下一个取样并将样本保存到对应的存储隔间，由此实现现场连续取样。When holding the sample, first move the cover plate as shown in the figure to ensure that the sample lowering hole is exposed, pull the operating lever back above the sample lowering hole, unscrew the external thread to close the If the operating lever is taken out, the sampling tube carrying the sample falls into the corresponding storage compartment for storage, and the cover plate is moved to cover the sample injection hole to close the corresponding storage compartment. Repeat the above operation in the next operating compartment to complete the next sampling and save the sample to the corresponding storage compartment, thereby realizing continuous sampling on site.
待需要使用已取的样本时，只需将所述抽屉抽出来即可取出含有样本的取样管，样本用完后清洗取样管即可再次将取样管安装到所述操作隔间，也可以更换新的取样管到所述操作隔间，重复上述步骤即可进行下一批次取样。When you need to use the sample you have taken, you only need to pull out the drawer to take out the sampling tube containing the sample. After the sample is used up, you can clean the sampling tube and install the sampling tube in the operating compartment again, or you can replace it A new sampling tube is sent to the operating compartment, and the above steps are repeated to carry out the next batch of sampling.
该装置具有操作简单、效率高、样本保存完好、取样量少等优点，因此，本发明将提供一种高效率、适用性广、取样量小的韧皮部微量组织取样装置，并达到避免高温坏死的效果。The device has the advantages of simple operation, high efficiency, well-preserved samples, and less sampling volume. Therefore, the present invention will provide a phloem micro-tissue sampling device with high efficiency, wide applicability, and small sampling volume, and achieve the goal of avoiding high-temperature necrosis. Effect.
附图说明Description of drawings
图1为微量组织取样装置的结构示意图。Fig. 1 is a schematic structural diagram of a micro-tissue sampling device.
图2为微量组织取样装置中操作隔间结构示意图。Fig. 2 is a schematic diagram of the structure of the operating compartment in the micro-tissue sampling device.
图3为操作隔间两侧的挡板结构示意图。Figure 3 is a schematic diagram of the structure of the baffles on both sides of the operating compartment.
图4为操作隔间的底板结构示意图。Fig. 4 is a schematic diagram of the bottom plate structure of the operating compartment.
图5为存储区中的抽屉结构示意图。Fig. 5 is a schematic diagram of the drawer structure in the storage area.
图6为微量组织取样装置中取样管剖面图。Fig. 6 is a sectional view of the sampling tube in the micro-tissue sampling device.
图7为微量组织取样装置中操作杆剖面图。Fig. 7 is a sectional view of the operating rod in the micro-tissue sampling device.
图8为微量组织取样时的操作示意图。Fig. 8 is a schematic diagram of operation when sampling micro-tissues.
图9为微量组织取样后操作取样管存入存储区的的示意图。Fig. 9 is a schematic diagram of storing the sampling tube into the storage area after micro-tissue sampling.
具体实施方式Detailed ways
以下将结合附图，对本发明的优选实施例进行详细的描述：应当理解，优选实施例仅为了说明本发明，而不是为了限制本发明的保护范围。The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings: It should be understood that the preferred embodiments are only for illustrating the present invention, rather than limiting the protection scope of the present invention.
如图1所示，本发明涉及一种树体韧皮部微量组织取样装置，包括框体1、存储区2和操作区3，其特征在于：所述框体1为长方体结构，所述存储区2设置在所述框体1的底部，所述操作区3固定在所述框体1上部空间；所述存储区2采用抽屉式结构推拉设计，所述存储区2前面板上设置有拉手4；所述操作区3分割为多组如图2所示的固定的操作隔间5，每个所述操作隔间5由左右两侧的两块如图3所示的挡板5.1和所述框体1前后两条边闭合形成操作空间，操作隔间5远离拉手4的一侧是开放式的，以便于取样操作，每个操作隔间可以单独实施操作，所述挡板5.1嵌入并固定连接到所述框体1的前后两个面上，所述操作隔间5远离所述拉手4一侧嵌入可更换取样管6，所述操作隔间5底部固定安装有如图4所示的底板5.2用来支撑所述取样管6以及用来隔离操作区和存储区，所述操作隔间5两侧的挡板侧面安装一段导轨5.3，所述取样管6安装到所述导轨5.3上，所述取样管6可以在所述导轨5.3上前后自由滑动，所述底板5.2靠近所述拉手4一侧设置有下样孔5.4，所述下样孔5.4正下方为所述存储区2，所述下样孔5.4设置有盖板5.5，所述盖板5.5安装在所述底板5.2下面，所述盖板5.5可以通过嵌入凹槽等方式嵌入到所述操作区3的所述挡板5.1上，所述盖板5.5可在所述底板下面前后拨动，所述盖板5.5拨动到所述下样孔5.4下方时正好将所述下样孔5.4封闭，要保存取样时用手或螺丝刀等工具拨动所述盖板5.5将下样孔5.4露出，所述取样管6滑动到所述下样孔5.4正上方时脱离导轨可以进入到所述存储区2。As shown in Fig. 1, the present invention relates to a kind of microtissue sampling device of tree body phloem, comprising frame body 1, storage area 2 and operation area 3, it is characterized in that: described frame body 1 is cuboid structure, and described storage area 2 It is arranged at the bottom of the frame body 1, and the operation area 3 is fixed in the upper space of the frame body 1; the storage area 2 adopts a drawer-type push-pull design, and a handle 4 is arranged on the front panel of the storage area 2; The operating area 3 is divided into multiple groups of fixed operating compartments 5 as shown in FIG. The front and rear sides of the body 1 are closed to form an operating space. The side of the operating compartment 5 away from the handle 4 is open to facilitate sampling operations. Each operating compartment can be operated independently. The baffle 5.1 is embedded and fixedly connected On the front and rear sides of the frame body 1, the operating compartment 5 is embedded with a replaceable sampling tube 6 on the side away from the handle 4, and the bottom of the operating compartment 5 is fixedly equipped with a bottom plate 5.2 as shown in Figure 4 Used to support the sampling tube 6 and to isolate the operating area and storage area, a section of guide rail 5.3 is installed on the side of the baffle plate on both sides of the operating compartment 5, and the sampling tube 6 is installed on the guide rail 5.3. The sampling tube 6 can freely slide back and forth on the guide rail 5.3, the bottom plate 5.2 is provided with a sample lowering hole 5.4 on the side close to the handle 4, the storage area 2 is directly below the sample lowering hole 5.4, and the lower The sample hole 5.4 is provided with a cover plate 5.5, and the cover plate 5.5 is installed under the bottom plate 5.2, and the cover plate 5.5 can be embedded into the baffle plate 5.1 of the operation area 3 by embedding grooves, etc. The cover plate 5.5 can be moved back and forth under the bottom plate. When the cover plate 5.5 is moved to the bottom of the sample hole 5.4, it will just seal the sample hole 5.4. When sampling, use tools such as hands or a screwdriver to save the sample. The cover plate 5.5 is moved to expose the sample lowering hole 5.4, and when the sampling tube 6 slides directly above the sample lowering hole 5.4, it is detached from the guide rail and can enter the storage area 2 .
树体韧皮部微量取样装置，其特征在于：所述存储区2设计为如图5所示推拉式抽屉7安装在所述框体1的底部，便于批量取出样本，所述抽屉7分为前后两部分，前端设置有多组存储隔间7.1，一组所述存储隔间对应正上方的一组所述操作隔间5，能分别将不同操作隔间获得的样本存放在对应的存储隔间内，防止样本交叉接触，便于取样人员能连续取样，且能保存不同来源的样本；所述抽屉7后部为冷却区7.2，所述冷却区7.2装入冰块或液氮即可实现对样本进行低温保存，可用于迅速冷冻生物组织，防止生物组织被破坏。The tree body phloem micro-sampling device is characterized in that: the storage area 2 is designed as a push-pull drawer 7 installed on the bottom of the frame 1 as shown in Figure 5, which is convenient for taking out samples in batches, and the drawer 7 is divided into front and rear two. part, the front end is provided with multiple sets of storage compartments 7.1, one set of storage compartments corresponds to a set of operation compartments 5 directly above, and samples obtained from different operation compartments can be stored in corresponding storage compartments , to prevent cross-contact of samples, which is convenient for sampling personnel to take samples continuously, and can store samples from different sources; the rear part of the drawer 7 is a cooling area 7.2, and the cooling area 7.2 can be filled with ice cubes or liquid nitrogen to realize the samples. Low temperature preservation can be used to rapidly freeze biological tissues to prevent biological tissues from being destroyed.
所述取样管6一侧顶部设置如图6所示螺纹孔6.1，所述取样管6另一侧设置为取样针头形状，所述取样管6由如图7所示操作杆8进行控制取样或进入存储区，所述操作杆8底部设置有外螺纹8.1，所述操作杆8通过所述外螺纹8.1对接拧入所述螺纹孔6.1而连接到所述取样管6上，从而通过所述操作杆8可以推拉所述取样管6在导轨上前后移动，所述操作杆8推拉到所述下样孔5.4上方时连同所述取样管6一起落入到对应的所述存储隔间7.1，旋开所述外螺纹8.1取出所述操作杆8，则所述取样管6保存到了所述存储隔间7.1，拨动所述盖板5.5到所述下样孔5.4下方即可将所述存储隔间7.1封闭起来。The top of one side of the sampling tube 6 is provided with a threaded hole 6.1 as shown in Figure 6, and the other side of the sampling tube 6 is in the shape of a sampling needle. Entering the storage area, the bottom of the operating rod 8 is provided with an external thread 8.1, and the operating rod 8 is connected to the sampling tube 6 through the butt screwing of the external thread 8.1 into the threaded hole 6.1, so that through the operation The rod 8 can push and pull the sampling tube 6 to move back and forth on the guide rail. When the operating rod 8 is pushed and pulled above the sample lowering hole 5.4, it will drop into the corresponding storage compartment 7.1 together with the sampling tube 6. Open the external thread 8.1 and take out the operating rod 8, then the sampling tube 6 is stored in the storage compartment 7.1, and the storage compartment can be removed by moving the cover plate 5.5 to the bottom of the sampling hole 5.4. Room 7.1 is closed.
所述取样管6的取样针头可以是锥形刀口6.1、斜切刀口6.2或弧切刀口6.3等形状，便于针对不同硬度和厚度的树体选择合适的取样针头，所述取样针头上面设置刻度线，可实现对目标树体取样深度的定量。The sampling needle of described sampling pipe 6 can be the shape such as tapered knife-edge 6.1, beveled knife-edge 6.2 or arc-cut knife-edge 6.3, is convenient to select suitable sampling needle for the tree body of different hardness and thickness, and scale line is arranged on described sampling needle , which can realize the quantification of the sampling depth of the target tree body.
取样前，在所述抽屉7的冷却区7.2中装入冰块或液氮等冷却剂，将所述抽屉7装入所述框体1底部的存储区2中，拨动所述盖板5.5将所述下样孔5.4封闭以免污染所述存储区2，将所述取样管6装入到所述操作隔间5中，将所述操作杆8通过所述外螺纹8.1连接到所述取样管6上。Before sampling, put ice cubes or liquid nitrogen and other coolants in the cooling area 7.2 of the drawer 7, put the drawer 7 into the storage area 2 at the bottom of the frame body 1, and turn the cover plate 5.5 Seal the sample lowering hole 5.4 so as not to pollute the storage area 2, install the sampling tube 6 into the operating compartment 5, and connect the operating rod 8 to the sampling port through the external thread 8.1. on tube 6.
取样时，如图8所示，推拉所述操作杆8使所述取样管6沿所述导轨往前移动至所述取样针头露出所述框体1，继续推动所述操作杆8即可将所述取样针头扎入树体9韧皮部进行取样，取样完成后往后拉所述操作杆9使所述取样管6离开所述树体9。When sampling, as shown in Figure 8, push and pull the operating rod 8 to move the sampling tube 6 forward along the guide rail until the sampling needle exposes the frame body 1, and continue to push the operating rod 8 to The sampling needle is pierced into the phloem of the tree body 9 for sampling, and after the sampling is completed, the operating rod 9 is pulled back to make the sampling tube 6 leave the tree body 9 .
保持样本时，如图9所示先拨动所述盖板5.5，确保将所述下样孔5.4露出来，将所述操作杆8推拉到所述下样孔5.4上方时连同所述取样管6一起落入到对应的所述存储隔间7.1，旋开所述外螺纹8.1取出所述操作杆8，则所述取样管6保存到了所述存储隔间7.1，拨动所述盖板5.5将所述下样孔5.4盖上以封闭对应的所述存储隔间7.1。在下一个操作隔间重复上述操作即可完成下一个取样并将样本保存到对应的存储隔间，由此实现现场连续取样。When holding the sample, first move the cover plate 5.5 as shown in Figure 9 to ensure that the sample lowering hole 5.4 is exposed, and push and pull the operating rod 8 above the sample lowering hole 5.4 together with the sampling tube 6 fall into the corresponding storage compartment 7.1 together, unscrew the external thread 8.1 to take out the operating rod 8, then the sampling tube 6 is stored in the storage compartment 7.1, turn the cover plate 5.5 The sample injection hole 5.4 is covered to close the corresponding storage compartment 7.1. Repeat the above operation in the next operating compartment to complete the next sampling and save the sample to the corresponding storage compartment, thereby realizing continuous sampling on site.
待需要使用已取的样本时，只需将所述抽屉7抽出来即可取出含有样本的取样管，样本用完后清洗取样管即可再次将取样管安装到所述操作隔间5，也可以更换新的取样管到所述操作隔间5，重复上述步骤即可进行下一批次取样。When the sample that has been taken needs to be used, the sampling tube containing the sample can be taken out only by pulling out the drawer 7, and the sampling tube can be installed in the operating compartment 5 again after the sample is used up. A new sampling tube can be replaced to the operating compartment 5, and the next batch of sampling can be performed by repeating the above steps.
虽然结合附图描述了本发明的实施方式，但是本领域技术人员可以在不脱离本发明的精神和范围的情况下做出各种修改和变型，这样的修改和变型均落入由所附权利要求所限定的范围之内。Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art can make various modifications and variations without departing from the spirit and scope of the present invention. within the bounds of the requirements.
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