CN120365757A - Molecular weight controllable silk fibroin profile and preparation method and application thereof - Google Patents
Molecular weight controllable silk fibroin profile and preparation method and application thereofInfo
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Abstract
The invention belongs to the field of implantable medical devices, and relates to a controllable molecular weight silk fibroin profile, a preparation method and application thereof, wherein the method comprises the steps of adding cellulose derivatives into silk fibroin salt solutions with different molecular weights to swell; the narrow-distribution controllable high molecular weight silk fibroin salt solution in the silk fibroin salt solutions with different molecular weights is prepared from 10 kDa-80 kDa, 80 kDa-100 kDa, 100 kDa-120 kDa, 120 kDa-160 kDa, 160 kDa-200 kDa and >200kDa. Loading the swelled silk fibroin salt solution into dialysis bags with specific shape and size, desalting in deionized water to form gel, and finally taking out the gel, and slowly drying and shaping under constant temperature and humidity environment to obtain silk fibroin hard section. The prepared silk fibroin profile has the characteristics of good strength, greenness, no toxicity, easy amplification and the like, can be further used in the field of implantable medical devices, has high efficiency and quick molding, and is more suitable for mass production and industrial production.
Description
Technical Field
The invention relates to the field of implantable medical devices, in particular to a controllable molecular weight silk fibroin profile, a preparation method and application thereof.
Background
Currently, the number of patients with bone defect repair and internal fixation of fractures is rapidly increasing, and patients are placing higher demands on safe and effective implants and implantable medical devices. Bone repair screws are commonly used internal fixation devices, playing an irreplaceable role in bone defect repair and functional reconstruction.
The silk fibroin is an extract of natural silk, has excellent mechanical property and physicochemical property after modification, has good biocompatibility and biodegradability of human body, and has no toxic or harmful effect on human body because of the degradation products of amino acid and polypeptide. And the material is widely applied to the field of biomedical materials because of rich sources and easy preparation. Biological research shows that the silk fibroin has good bone induction activity, can promote the biological functions of growth, proliferation, differentiation and the like of osteoblasts, and gradually becomes an excellent candidate material for preparing bone repair screws. At present, the research on manufacturing bone implant materials by using silk fibroin is mainly focused on optimizing and improving the mechanical and biological compatibility of the materials, optimizing the osteoinductive and bone integration properties of the materials, and mainly focusing on a novel preparation method and coating and modifying the surfaces of metal materials.
Silk fibroin material is mainly prepared from silk cocoons through degumming, dissolving, dialyzing and modifying operations. Soft materials such as suture lines, gel application and the like which are woven and manufactured by degummed silk have been widely studied and applied in clinic, but silk fibroin hard materials still have a plurality of difficulties in the process. On one hand, the preparation of the silk fibroin hard material needs to solve the key technical problems of bubbles, large-size preparation, fragile mechanical properties and the like in the traditional process, and on the other hand, because in the production process of the traditional silk fibroin hard material, toxic gas components such as hexafluoroisopropanol exist, and the hard material prepared by solution drying has smaller size and cannot be industrially popularized. Therefore, the production process of the silk fibroin hard material is improved, the realization performance is better, and the preparation of the green nontoxic silk fibroin material has wide market prospect.
Disclosure of Invention
Aiming at the problems that the existing silk fibroin hard material has poor strength, is slow to form, cannot realize large-size manufacturing and the like, the invention aims to provide a preparation method of a silk fibroin profile with controllable molecular weight.
In order to achieve the above purpose, the controllable molecular weight silk fibroin profile and the preparation method thereof provided by the invention comprise the following steps:
s1, preparing a silk fibroin salt solution, wherein the silk fibroin salt solution comprises silk fibroin and cellulose derivatives, S2, forming gel, S3, drying and shaping;
The preparation method does not comprise any physical crosslinking agent and chemical crosslinking agent, preferably does not adopt any crosslinking method for crosslinking, and also does not comprise any other auxiliary agent, and most preferably the profile consists of silk fibroin and cellulose derivatives.
The method comprises the following steps:
S1, adding cellulose derivatives into silk fibroin salt solutions with different molecular weights (preferably, wherein the concentration of silk fibroin is 30mg/ml-200 mg/ml) to swell to obtain a swelled solution, wherein the salt solution of the narrow-distribution controllable high-molecular-weight silk fibroin is prepared by adding cellulose derivatives into silk fibroin salt solutions with the index average molecular weights (preferably, measured by a rheological method) of 10 kDa-80 kDa, 80 kDa-100 kDa, 100 kDa-120 kDa, 120 kDa-160 kDa, 160 kDa-200 kDa and >200kDa, and preferably, the concentration of silk fibroin in the silk fibroin salt solution is 30mg/ml-200mg/ml, and the salt solution preferably further comprises lithium bromide;
S2, filling the swollen solution into a dialysis bag with a specific shape and size, and desalting in deionized water to form gel;
And S3, placing the formed gel in a constant temperature and humidity environment, slowly standing, drying and shaping to obtain the silk fibroin profile, wherein the profile is preferably a hard profile.
Further, in the step S1, according to the mass ratio, the silk fibroin is cellulose derivative=6-12:0.2-1.5, the swelling environment is 2-8 ℃, the swelling time is 24-72 hours, wherein the silk fibroin salt solution is one or more of cellulose derivative I without desalting purification, and specific examples are as follows:
Cellulose derivatives, preferably one or more selected from the group consisting of carboxy cellulose, alkyl cellulose or hydroxy cellulose, more preferably sodium carboxymethyl cellulose, methyl cellulose, hydroxyethyl cellulose.
Further, in step S2, after the swelling is completed, the dialysis bag is placed in deionized water for desalting for 48-96 hours, wherein the deionized water is replaced every 1 hour until the solution in the dialysis bag is dialyzed to a gel state, and the shape of the dialysis bag can be one or more of spherical, cylindrical and elliptic.
In step S3, the environment used for drying and shaping is a constant temperature and humidity box, the constant temperature is 20-60 ℃, and the humidity is 20-40% rh.
The silk fibroin profile with controllable molecular weight is prepared by the method.
Furthermore, the silk fibroin profile can resist internal stress generated in the gelation and drying process, the Young modulus is 800-1200MPa, the density is 0.9-1.1g/cm 3, the density is close to the density of human bones, and the Young modulus is 1000-1200MPa.
Preferably, the silk fibroin profile does not include any physical crosslinking agent or chemical crosslinking agent, nor does it employ any crosslinking method. Nor any other auxiliary agent.
Further preferably, the silk fibroin profile consists of silk fibroin and cellulose derivatives.
The cellulose derivative is selected from one or more of carboxyl cellulose, alkyl cellulose or hydroxy cellulose, more preferably one or more of sodium carboxymethyl cellulose, methyl cellulose and hydroxyethyl cellulose;
further, the Young's modulus of the profile is 800-1200MPa, preferably 1000-1200MPa, and the density thereof is 0.9-1.1g/cm 3.
The application of the silk fibroin profile is characterized by comprising the steps of preparing a material in the biomedical field, wherein the prepared material in the biomedical field is used for bone repair materials, surgical sutures, tissue engineering scaffolds and degradable packaging materials.
Preferably, in step S1, the molecular weight of the silk fibroin salt solution used is (10 kDa-80 kDa, 80 kDa-100 kDa, 100 kDa-120 kDa, 120 kDa-160 kDa, 160 kDa-200 kDa, >200 kDa), and the test method refers to appendix B of the industry standard tissue engineering medical instrument silk fibroin (YY/T1950-2024), and the rheological data, viscosity data and the like are measured and imported into Favorsun SmartMolFit "complex intelligent computation" biological high molecular weight commercial software to calculate the number average molecular weight and the weight average molecular weight.
Preferably, in step S1, the mass ratio of silk fibroin to cellulose derivative=6 to 12:0.2 to 1.5. The swelling environment is 2-8 ℃, and the swelling time is 24-72 h. More preferably, the silk fibroin is cellulose derivative=8 to 12:0.8 to 1.2. The swelling environment is 2-8 ℃, and the swelling time is 48-72 h.
Preferably, in step S1, the cellulose derivative substance includes, but is not limited to, sodium carboxymethyl cellulose, methyl cellulose, hydroxyethyl cellulose, and other cellulose derivatives.
Preferably, in step S2, after the swelling is completed, the dialysis bag is placed in deionized water for desalting for 48-96 hours (wherein the deionized water is replaced every 1 hour until the solution in the dialysis bag is dialyzed to a gel state). It is further preferred that the dialysis bag is placed in deionized water for desalting for 72-96 hours (wherein deionized water is replaced every 1 hour until the solution in the dialysis bag is dialyzed to gel state).
Preferably, in step S2, the dialysis bag may be spherical, cylindrical, or elliptical in shape. Further preferably cylindrical.
Preferably, in the step S3, the formed gel is placed in a constant temperature and humidity environment for slow standing, drying and shaping, and the silk fibroin hard profile is obtained.
The hard section has higher rigidity and mechanical property close to that of natural cancellous bone (the Young modulus of cancellous bone is 1000MPa-2000 MPa).
The setting environment is that the constant temperature value in the constant temperature and humidity box is 20-60 ℃ and the humidity is 20-40% RH. Further preferably, the temperature is 35 to 45 ℃ and the humidity is 25 to 35% RH.
The cellulose derivatives of the present invention may function as follows:
(1) The cellulose derivative can help the silk protein form a uniform gel network and the crystallization area enhance the mechanical properties of the silk protein profile, such as improving strength, toughness or elastic modulus. This enhancement results from interactions between the cellulose derivative and the silk fibroin, such as chemical bonding, physical entanglement, or hydrogen bonding.
(2) The degradation rate is regulated, and the cellulose derivative optimizes the application of the cellulose derivative in the biomedical field by influencing the degradation rate of the silk fibroin. By adjusting the type, content or structure of the cellulose derivative, the degradation rate of the silk fibroin can be accurately controlled, so as to meet the requirements of repairing and regenerating different tissues.
Compared with the prior art, the invention has the following advantages:
(1) The silk fibroin profile can be prepared into a required profile by only silk fibroin and cellulose derivatives, and the steps are simple, and the required profile is processed into a specified shape through subsequent processing. The existing section is usually formed by processing a metal product, and compared with the metal product, the silk fibroin type implant body extracted from natural materials has fewer rejection reactions and better affinity.
(2) The silk fibroin profile with specific molecular weight can be synthesized according to specific requirements. For example, in the biomedical field, if the interaction between the silk fibroin profile and the receptor of a specific cell is required, the molecular weight can be customized according to the cell characteristics, so that the molecular weight has better biocompatibility, and the molecular weight is suitable for different medical application scenes, such as preparation of scaffold materials in tissue engineering, and the like.
(3) The cellulose derivative can help the silk protein form a uniform gel network and the crystallization area enhance the mechanical properties of the silk protein profile, such as improving strength, toughness or elastic modulus.
(4) The silk fibroin profile is degradable, and the degradation rate of all the silk fibroin profiles in the simulated actual blood environment can reach 50% or more at 24 weeks.
Drawings
FIG. 1 SEM image after gellation of the silk fibroin profile of example 1
FIG. 2A photograph of a silk fibroin profile of example 1 after dehydration molding
FIG. 3A picture of a silk fibroin profile of example 1 after processing
FIG. 4 graph of density versus Young's modulus data for silk fibroin profiles of examples 1-4 and comparative example 1
FIG. 5 summary of the silk fibroin profiles to mass residual ratios of examples 1-4 and comparative example 1
Detailed Description
The technical solution of the present invention will be further described by means of specific examples and drawings, it being understood that the specific examples described herein are only for aiding in understanding the present invention and are not intended to be limiting. And the drawings described herein are for illustration purposes only and are not intended to limit the scope of the present disclosure. Unless otherwise indicated, all materials used in the examples of the present invention are those commonly used in the art, and all methods used in the examples are those commonly used in the art.
Example 1:
(1) Swelling of silk fibroin, namely placing a certain amount of hydroxypropyl methylcellulose (HPMC) powder into a silk fibroin solution salt solution (wherein the molecular weight of the silk fibroin is 160 kDa-200 kDa and the concentration is 50 mg/ml), wherein the mass ratio of the silk fibroin to the HPMC is 9:1, and swelling for 48 hours at a temperature of 2-8 ℃ in a refrigerator for later use.
(2) Silk fibroin gel is prepared by placing silk fibroin solution in a dialysis bag after swelling is completed, placing the dialysis bag in deionized water, replacing the deionized water every 1 hour, and dialyzing for 48 hours, wherein the solution in the dialysis bag is in gel state, and the gel state product SEM is shown in figure 1.
(3) And (3) dehydrating and forming the section bar, namely uniformly dehydrating the gel obtained by dialysis in a constant temperature and humidity box, and setting the temperature and humidity to be 35 ℃ and 25% RH for 72 hours respectively to obtain a rod-shaped section bar finished product. A picture of the bar-shaped profile is shown in fig. 2, and a product obtained after simple processing is shown in fig. 3.
The density of the prepared profile is 0.98g/cm 3, and the Young's modulus is 1200MPa (shown in figure 4).
Example 2:
(1) Swelling of silk fibroin, namely placing a certain amount of hydroxypropyl methylcellulose (HPMC) powder into a silk fibroin solution salt solution (wherein the molecular weight of the silk fibroin is 160 kDa-200 kDa and the concentration is 50 mg/ml), wherein the mass ratio of the silk fibroin to the HPMC is 12:1, and swelling for 48 hours at a temperature of 2-8 ℃ in a refrigerator for later use.
(2) The silk fibroin gel is prepared by placing silk fibroin solution into a dialysis bag after swelling is completed, placing the dialysis bag into deionized water, replacing the deionized water every 1 hour, and dialyzing for 48 hours, wherein the solution in the dialysis bag is in gel state.
(3) And (3) dehydrating and forming the section bar, namely uniformly dehydrating the gel obtained by dialysis in a constant temperature and humidity box, and setting the temperature and humidity to be 35 ℃ and 25% RH for 72 hours respectively to obtain a rod-shaped section bar finished product.
(4) The density of the prepared profile is 0.99g/cm 3, and the Young's modulus is 1159MPa (shown in figure 4).
Example 3:
(1) Swelling of silk fibroin, namely placing a certain amount of carboxymethyl cellulose (CMC) powder into a silk fibroin solution salt solution (wherein the molecular weight of the silk fibroin is 160 kDa-200 kDa and the concentration is 50 mg/ml), wherein the mass ratio of the silk fibroin to the CMC is 9:1, and swelling for 48 hours at a temperature of 2-8 ℃ in a refrigerator for later use.
(2) The silk fibroin gel is prepared by placing silk fibroin solution into a dialysis bag after swelling is completed, placing the dialysis bag into deionized water, replacing the deionized water every 1 hour, and dialyzing for 48 hours, wherein the solution in the dialysis bag is in gel state.
(3) And (3) dehydrating and forming the section bar, namely uniformly dehydrating the gel obtained by dialysis in a constant temperature and humidity box, and setting the temperature and humidity to be 35 ℃ and 25% RH for 72 hours respectively to obtain a rod-shaped section bar finished product.
(4) The density of the prepared profile is 1.07g/cm 3, and the Young's modulus is 1174MPa (shown in figure 4).
Example 4:
(1) And (3) silk fibroin swelling, namely placing a certain amount of hydroxyethyl cellulose powder into a silk fibroin solution salt solution (wherein the molecular weight of the silk fibroin is 160 kDa-200 kDa, and the concentration is 50 mg/ml), wherein the mass ratio of the silk fibroin to the hydroxyethyl cellulose is 9:1, and swelling for 48 hours at a temperature of 2-8 ℃ in a refrigerator for later use.
(2) The silk fibroin gel is prepared by placing silk fibroin solution into a dialysis bag after swelling is completed, placing the dialysis bag into deionized water, replacing the deionized water every 1 hour, and dialyzing for 48 hours, wherein the solution in the dialysis bag is in gel state.
(3) And (3) dehydrating and forming the section bar, namely uniformly dehydrating the gel obtained by dialysis in a constant temperature and humidity box, and setting the temperature and humidity to be 35 ℃ and 25% RH for 72 hours respectively to obtain a rod-shaped section bar finished product.
(4) The density of the prepared profile is 1.02g/cm 3, and the Young's modulus is 1065MPa (see FIG. 4).
Comparative example 1 adjustment of the amount ratio of silk fibroin to cellulose
(1) Swelling of silk fibroin, namely placing a certain amount of hydroxypropyl methylcellulose (HPMC) powder into a silk fibroin solution salt solution (wherein the molecular weight of the silk fibroin is 160 kDa-200 kDa and the concentration is 50 mg/ml), wherein the mass ratio of the silk fibroin to the HPMC is 13:0.2, and swelling for 48 hours at a temperature of 2-8 ℃ in a refrigerator for later use.
(2) The silk fibroin gel is prepared by placing silk fibroin solution into a dialysis bag after swelling is completed, placing the dialysis bag into deionized water, replacing the deionized water every 1 hour, and dialyzing for 48 hours, wherein the solution in the dialysis bag is in gel state.
(3) And (3) dehydrating and forming the section bar, namely uniformly dehydrating the gel obtained by dialysis in a constant temperature and humidity box, and setting the temperature and humidity to be 35 ℃ and 25% RH for 72 hours respectively to obtain a rod-shaped section bar finished product.
(4) The density of the prepared section bar is 0.79g/cm 3, and Young's modulus is 840MPa (as shown in figure 4)
Comparative example 2 without addition of cellulose or cellulose derivatives
(1) Silk fibroin swelling, namely taking silk fibroin solution salt solution (wherein the molecular weight of the silk fibroin is 160 kDa-200 kDa, and the concentration is 50 mg/ml), and swelling for 48 hours at a temperature of 2-8 ℃ in a refrigerator for later use.
(2) Silk fibroin gel is prepared by placing silk fibroin solution in dialysis bag after swelling completely, and placing dialysis bag in deionized water, and replacing deionized water every 1 hr, there is no way to gel.
Comparative example 3 adjustment of swelling time
(1) Swelling of silk fibroin, namely placing a certain amount of hydroxypropyl methylcellulose (HPMC) powder into a silk fibroin solution salt solution (wherein the molecular weight of the silk fibroin is 160 kDa-200 kDa, and the concentration is 50 mg/ml), and the mass ratio of the silk fibroin to the HPMC is 9:1. Swelling for 10 hours at the temperature of 2-8 ℃ in a refrigerator for later use.
(2) Silk fibroin gel is prepared by placing silk fibroin solution in dialysis bag after swelling completely, and placing dialysis bag in deionized water, and replacing deionized water every 1 hr. A uniform gel state, a part of gel, and a part of solution state cannot be formed.
In the present invention, samples obtained in examples 1, 2, 3, and 4 and comparative example 1 were put in 200mL of a PBS solution containing 1% Sodium Dodecyl Sulfate (SDS) at ph=7.4 to simulate the actual blood environment for degradation of the product, and the weight of the 0-point profile was recorded and weighed at 2 weeks, 4 weeks, 8 weeks, 16 weeks and 24 weeks, respectively, to calculate the mass loss rate (see fig. 5). The data are shown in the table:
TABLE 1 summary of weight data for different time of silk fibroin profiles
| Initial weight (g) | Quality of 2 weeks (g) | 8 Weeks mass (g) | 16 Weeks mass (g) | 24 Week mass (g) | |
| Example 1 | 10.23 | 9.97 | 8.01 | 6.87 | 4.23 |
| Example 2 | 10.45 | 10.22 | 8.12 | 5.56 | 3.33 |
| Example 3 | 10.97 | 10.01 | 7.65 | 5.42 | 3.21 |
| Example 4 | 9.98 | 9.54 | 8.34 | 4.04 | 3.25 |
| Comparative example 1 | 11.02 | 9.87 | 5.43 | 3.21 | 1.01 |
As can be seen from the comparative examples and examples, the addition of the cellulose derivative and the complete swelling thereof can achieve 1000MPa to 1200MPa in Young's modulus (example 1, example 2, example 3 and example 4). Wherein the content of cellulose determines the Young's modulus data of the silk fibroin profile, the higher the content of cellulose in the case where the cellulose is the same cellulose (example 1 and example 2), the higher the Young's modulus thereof. HPMC degrades at a slower rate (examples 1, 2 and 3) with near cellulose content and can be used for longer periods.
The content and the type of cellulose directly influence the Young modulus of the profile, the swelling time influences the uniformity of the profile, the uniform gel cannot be formed after the complete swelling time of the cellulose is not reached, and the hard profile cannot be formed after the subsequent dehydration molding.
The higher the cellulose content, the greater the Young's modulus of the profile generally, as cellulose provides structural support and rigidity. Too low a content may result in insufficient strength of the profile. Different kinds of cellulose (e.g., microcrystalline cellulose, nanocellulose, etc.) have different effects on young's modulus due to their different degree of polymerization, crystallinity, and molecular arrangement. High crystallinity cellulose generally provides higher stiffness.
Swelling is the process of swelling cellulose in solvent by absorbing water, so that molecular chains are loosened, and the subsequent forming is facilitated. The swelling time is insufficient, and cellulose cannot be fully swelled, so that gel is uneven, and the physical properties of the profile are affected. Too long a swelling time, while ensuring adequate swelling, may cause cellulose degradation or other side effects, with a balance being found between adequate swelling and avoiding degradation. As is clear from a comparison of example 1 and comparative example 3, comparative example 3 has too short a swelling time to form a uniform gel, and cannot form a hard profile in the following.
The uniform gel is the basis of the subsequent dehydration molding, and ensures the consistency of the density and the strength of the section bar at all positions. If the gel is uneven, the profile after dehydration molding can have the problems of stress concentration, deformation or cracking.
The dehydration process is to remove excess water so that the cellulose molecules are tightly combined to form a hard structure. After dehydration, the cellulose molecules are closely arranged to form a high-density and high-strength hard profile.
The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A preparation method of a silk fibroin profile with controllable molecular weight is characterized by comprising the following steps of S1, silk fibroin salt solution preparation, S2, gel formation and S3, drying and shaping.
2. The method of manufacturing according to claim 1, characterized in that:
S1, adding cellulose derivatives into silk fibroin salt solutions with different molecular weights (preferably, wherein the concentration of silk fibroin is 30mg/ml-200 mg/ml) to swell to obtain a swelled solution, wherein the salt solution of the narrow-distribution controllable high-molecular-weight silk fibroin is prepared by adding cellulose derivatives into silk fibroin salt solutions with the index average molecular weights (preferably, measured by a rheological method) of 10 kDa-80 kDa, 80 kDa-100 kDa, 100 kDa-120 kDa, 120 kDa-160 kDa, 160 kDa-200 kDa and >200kDa, and preferably, the concentration of silk fibroin in the silk fibroin salt solution is 30mg/ml-200mg/ml, and the salt solution preferably further comprises lithium bromide;
S2, filling the swollen solution into a dialysis bag with a specific shape and size, and desalting in deionized water to form gel;
And S3, placing the formed gel in a constant temperature and humidity environment, slowly standing, drying and shaping to obtain the silk fibroin profile, wherein the profile is preferably a hard profile.
3. The preparation method according to claim 1, wherein in the step S1, the silk fibroin is cellulose derivative=6-12:0.2-1.5, the swelling environment is 2-8 ℃, the swelling time is 24-72h, wherein the silk fibroin salt solution is one or more of cellulose derivative I without desalting purification, and the specific examples are as follows:
Cellulose derivatives, preferably one or more selected from the group consisting of carboxy cellulose, alkyl cellulose or hydroxy cellulose, more preferably sodium carboxymethyl cellulose, methyl cellulose, hydroxyethyl cellulose.
4. The method according to claim 1, wherein in step S2, after swelling is completed, the dialysis bag is placed in deionized water for desalting for 48-96 hours, and deionized water is replaced every 1 hour until the solution in the dialysis bag is dialyzed to a gel state, wherein the dialysis bag can be one or more of spherical, cylindrical and elliptical in shape.
5. The preparation method according to claim 1, wherein in the step S3, the environment used for drying and shaping is a constant temperature and humidity box with a constant temperature of 20-60 ℃ and a humidity of 20-40% rh.
6. A controllable molecular weight silk fibroin profile is characterized in that the profile is prepared by the preparation method according to one of claims 1-5.
7. A profile as defined in claim 6, characterized in that its Young's modulus is 800-1200MPa, preferably 1000-1200MPa, and its density is 0.9-1.1g/cm 3.
8. Profile according to one of claims 6-7, characterized in that it does not comprise any physical as well as chemical cross-linking agents, preferably does not use any cross-linking method for cross-linking, and still preferably does not comprise any other auxiliary agent, most preferably consists of silk fibroin and cellulose derivatives.
9. A controlled molecular weight silk fibroin profile, characterized by comprising silk fibroin and cellulose derivatives, preferably without any physical cross-linking agent and chemical cross-linking agent, further preferably without any cross-linking method, yet further preferably without any other auxiliary agent, most preferably consisting of silk fibroin and cellulose derivatives, said cellulose derivatives being selected from one or more of carboxy cellulose, alkyl cellulose or hydroxy cellulose, more preferably from one or more of sodium carboxymethyl cellulose, methyl cellulose, hydroxyethyl cellulose;
further, the Young's modulus of the profile is 800-1200MPa, preferably 1000-1200MPa, and the density thereof is 0.9-1.1g/cm 3.
10. Use of a controlled molecular weight silk fibroin profile prepared by the preparation method according to one of claims 1-5 or a controlled molecular weight silk fibroin profile according to one of claims 6-9, characterized in that the use comprises the preparation of biomedical field materials, preferably the prepared biomedical field materials for bone repair materials, surgical sutures, tissue engineering scaffolds and degradable packaging materials.
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